Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059498.t1 | XP_003607897.1 | 98.3 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 5.80E-60 | 240 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059498.t1 | Q8GY89 | 76.7 | 120 | 28 | 0 | 1 | 120 | 1 | 120 | 7.5e-48 | 191.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059498.t1 | Q2MGR0 | 98.3 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 4.2e-60 | 240.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059498 | MS.gene059499 | 0.822224 | 2.66E-53 | -1.69E-46 |
| MS.gene059498 | MS.gene059501 | 0.989072 | 1.42E-176 | -1.69E-46 |
| MS.gene059498 | MS.gene059502 | 0.8208 | 5.66E-53 | -1.69E-46 |
| MS.gene059498 | MS.gene43489 | 0.800419 | 1.45E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059498.t1 | MTR_4g084180 | 98.333 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 4.67e-85 | 243 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059498.t1 | AT1G31170 | 76.667 | 120 | 28 | 0 | 1 | 120 | 1 | 120 | 1.24e-54 | 167 |
| MS.gene059498.t1 | AT1G31170 | 76.667 | 120 | 28 | 0 | 1 | 120 | 1 | 120 | 1.95e-54 | 166 |
| MS.gene059498.t1 | AT1G31170 | 76.667 | 120 | 28 | 0 | 1 | 120 | 1 | 120 | 1.95e-54 | 166 |
| MS.gene059498.t1 | AT1G31170 | 76.667 | 120 | 28 | 0 | 1 | 120 | 1 | 120 | 1.20e-53 | 167 |
| MS.gene059498.t1 | AT1G31170 | 75.000 | 120 | 28 | 1 | 1 | 120 | 1 | 118 | 9.44e-51 | 157 |
Find 32 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAAATTTAGGGGCTCTTTC+TGG | 0.208234 | 4.3:+27667739 | MS.gene059498:intron |
| GTGAAACTCCTCAAAGTATT+TGG | 0.243580 | 4.3:-27667593 | None:intergenic |
| GGGGCTCTTTCTGGTGGTTC+TGG | 0.256526 | 4.3:+27667748 | MS.gene059498:intron |
| ATGGATAGTATTAGTGAAAT+TGG | 0.282662 | 4.3:+27667874 | MS.gene059498:CDS |
| AAACAAAGTTCAAGAACTTA+TGG | 0.307753 | 4.3:+27667855 | MS.gene059498:CDS |
| CTTGTGGTCGTAGGTTTCTC+TGG | 0.347484 | 4.3:+27669156 | MS.gene059498:intron |
| CTTTCTGGTGGTTCTGGAAG+TGG | 0.365172 | 4.3:+27667754 | MS.gene059498:CDS |
| TGGTGTGGGTCCAGTGATAC+TGG | 0.431666 | 4.3:+27667777 | MS.gene059498:CDS |
| GTGGCACAAAAGAGACTCTA+AGG | 0.431947 | 4.3:+27669241 | MS.gene059498:CDS |
| AAATGAGTGATGCACCATTA+GGG | 0.432228 | 4.3:-27667634 | None:intergenic |
| ATTGATGTGCTCGAGGTTGA+TGG | 0.440259 | 4.3:+27668924 | MS.gene059498:CDS |
| GAGGTTGATGGAAACTATTA+TGG | 0.443314 | 4.3:+27668936 | MS.gene059498:CDS |
| GGTTCTGGAAGTGGTGGTGT+GGG | 0.445849 | 4.3:+27667763 | MS.gene059498:CDS |
| CCACCATACGCTGCAAAATA+CGG | 0.452823 | 4.3:+27669217 | MS.gene059498:CDS |
| TGCAGGTTCCAAATACTTTG+AGG | 0.453361 | 4.3:+27667585 | MS.gene059498:CDS |
| TGGTTCTGGAAGTGGTGGTG+TGG | 0.464993 | 4.3:+27667762 | MS.gene059498:CDS |
| TTTGATCTAGTCCTCATCAT+AGG | 0.468493 | 4.3:-27667826 | None:intergenic |
| TCTGCTTCATCCAACCCTAA+TGG | 0.481086 | 4.3:+27667620 | MS.gene059498:CDS |
| TTCCTCTTGATAAGATAAGA+AGG | 0.501249 | 4.3:+27667803 | MS.gene059498:CDS |
| AGAGGAAGTTCCAGTATCAC+TGG | 0.512026 | 4.3:-27667787 | None:intergenic |
| TAGGTTTCTCTGGCTGTCAC+CGG | 0.529110 | 4.3:+27669166 | MS.gene059498:intron |
| TACGAGGCTCACCAGCGACT+TGG | 0.534931 | 4.3:+27669189 | MS.gene059498:CDS |
| AATTTAGGGGCTCTTTCTGG+TGG | 0.550422 | 4.3:+27667742 | MS.gene059498:intron |
| GGCCTTCTTATCTTATCAAG+AGG | 0.554502 | 4.3:-27667805 | None:intergenic |
| GTCGCTGGTGAGCCTCGTAC+CGG | 0.559622 | 4.3:-27669185 | None:intergenic |
| TCTGGTGGTTCTGGAAGTGG+TGG | 0.571088 | 4.3:+27667757 | MS.gene059498:CDS |
| CTCTGGCTGTCACCGGTACG+AGG | 0.572203 | 4.3:+27669173 | MS.gene059498:CDS |
| TGGTGGGGAGTCCAAGTCGC+TGG | 0.572299 | 4.3:-27669200 | None:intergenic |
| AGATAAGAAGGCCTATGATG+AGG | 0.627844 | 4.3:+27667815 | MS.gene059498:CDS |
| GAGTGATGCACCATTAGGGT+TGG | 0.637639 | 4.3:-27667630 | None:intergenic |
| ATACGCTGCAAAATACGGCG+TGG | 0.718853 | 4.3:+27669222 | MS.gene059498:CDS |
| GATGCAGATTGATGTGCTCG+AGG | 0.724443 | 4.3:+27668917 | MS.gene059498:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAATTATTTTTAAAATTT+AGG | + | chr4.3:27667727-27667746 | MS.gene059498:intron | 0.0% |
| !!! | ATAATTATTTTTAAAATTTA+GGG | + | chr4.3:27667728-27667747 | MS.gene059498:intron | 0.0% |
| !!! | TGATTTCTTATTCTTTTTTT+TGG | - | chr4.3:27668496-27668515 | None:intergenic | 15.0% |
| !!! | TTACTTTTCTAAATTTTGTA+TGG | + | chr4.3:27668962-27668981 | MS.gene059498:intron | 15.0% |
| !!! | TTATTTTAACAAAAAAGAGT+AGG | - | chr4.3:27668841-27668860 | None:intergenic | 15.0% |
| !!! | TTTTGATCTAAATATTTCAT+TGG | - | chr4.3:27668320-27668339 | None:intergenic | 15.0% |
| !! | AAATGAATATTAGAACCTTA+AGG | - | chr4.3:27668027-27668046 | None:intergenic | 20.0% |
| !! | AATGAATATTAGAACCTTAA+GGG | - | chr4.3:27668026-27668045 | None:intergenic | 20.0% |
| !! | AGAAATATATAAACTCCAAA+AGG | - | chr4.3:27667963-27667982 | None:intergenic | 20.0% |
| !! | CTATATAATTATTCTGTGAT+GGG | + | chr4.3:27668660-27668679 | MS.gene059498:intron | 20.0% |
| !! | TCTATATAATTATTCTGTGA+TGG | + | chr4.3:27668659-27668678 | MS.gene059498:intron | 20.0% |
| !! | TTAATATCAAAGAAAATGAC+AGG | - | chr4.3:27668265-27668284 | None:intergenic | 20.0% |
| !!! | CAAAATCTATTTATACCTTT+TGG | + | chr4.3:27667945-27667964 | MS.gene059498:intron | 20.0% |
| !!! | CTTTGTTAGACTTTTTTTTT+AGG | + | chr4.3:27668056-27668075 | MS.gene059498:intron | 20.0% |
| ! | AAACAAAGTTCAAGAACTTA+TGG | + | chr4.3:27667855-27667874 | MS.gene059498:CDS | 25.0% |
| ! | AGTCATATACAATACAGTTA+TGG | + | chr4.3:27668112-27668131 | MS.gene059498:intron | 25.0% |
| ! | ATATTAATCTTTGTAGCTGA+GGG | + | chr4.3:27668278-27668297 | MS.gene059498:intron | 25.0% |
| ! | ATGAATATTAGAACCTTAAG+GGG | - | chr4.3:27668025-27668044 | None:intergenic | 25.0% |
| ! | ATGGATAGTATTAGTGAAAT+TGG | + | chr4.3:27667874-27667893 | MS.gene059498:CDS | 25.0% |
| ! | ATTGTTTATAGTGATAAGCA+CGG | - | chr4.3:27669029-27669048 | None:intergenic | 25.0% |
| ! | TAATTACATGTGTGAGTATT+AGG | + | chr4.3:27668173-27668192 | MS.gene059498:intron | 25.0% |
| ! | TACAATACAGTTATGGAAAA+TGG | + | chr4.3:27668119-27668138 | MS.gene059498:intron | 25.0% |
| ! | TTCATCAAAATTATCTTGCT+CGG | + | chr4.3:27668869-27668888 | MS.gene059498:intron | 25.0% |
| !! | ATGTCGATTATATTATCTTG+TGG | + | chr4.3:27669140-27669159 | MS.gene059498:intron | 25.0% |
| !!! | AGTTCTATTGTTTTGAAAGT+GGG | + | chr4.3:27668533-27668552 | MS.gene059498:intron | 25.0% |
| !!! | TAGTTCTATTGTTTTGAAAG+TGG | + | chr4.3:27668532-27668551 | MS.gene059498:intron | 25.0% |
| AGACCTAGAGAAAATGATAA+AGG | - | chr4.3:27668345-27668364 | None:intergenic | 30.0% | |
| ATAGAAATTGTTGACACACA+GGG | - | chr4.3:27668380-27668399 | None:intergenic | 30.0% | |
| ATCTCAATAGTATTCAACAG+TGG | - | chr4.3:27668700-27668719 | None:intergenic | 30.0% | |
| ATTTAAAGAGAATCCCCTTA+AGG | + | chr4.3:27668009-27668028 | MS.gene059498:intron | 30.0% | |
| GATATTAATCTTTGTAGCTG+AGG | + | chr4.3:27668277-27668296 | MS.gene059498:intron | 30.0% | |
| TACAGTGAAAATAGCTATAG+TGG | - | chr4.3:27668629-27668648 | None:intergenic | 30.0% | |
| TCTCAATAGTATTCAACAGT+GGG | - | chr4.3:27668699-27668718 | None:intergenic | 30.0% | |
| TGTGTCAACAATTTCTATGT+TGG | + | chr4.3:27668382-27668401 | MS.gene059498:intron | 30.0% | |
| TTATATTATCTTGTGGTCGT+AGG | + | chr4.3:27669147-27669166 | MS.gene059498:intron | 30.0% | |
| TTCCTCTTGATAAGATAAGA+AGG | + | chr4.3:27667803-27667822 | MS.gene059498:CDS | 30.0% | |
| ! | AGACCTTTATCATTTTCTCT+AGG | + | chr4.3:27668339-27668358 | MS.gene059498:intron | 30.0% |
| ! | TATCATTTTCTCTAGGTCTT+AGG | + | chr4.3:27668346-27668365 | MS.gene059498:intron | 30.0% |
| !!! | GTTCTATTGTTTTGAAAGTG+GGG | + | chr4.3:27668534-27668553 | MS.gene059498:intron | 30.0% |
| AAAATGAGTGATGCACCATT+AGG | - | chr4.3:27667638-27667657 | None:intergenic | 35.0% | |
| AAATGAGTGATGCACCATTA+GGG | - | chr4.3:27667637-27667656 | None:intergenic | 35.0% | |
| AAGAGAGAGTAAGTGATTAC+AGG | - | chr4.3:27667910-27667929 | None:intergenic | 35.0% | |
| AATTGGTCAGTAGTTAGAGA+TGG | + | chr4.3:27668581-27668600 | MS.gene059498:intron | 35.0% | |
| ACAGTATCCCTTAACAAACA+GGG | - | chr4.3:27668743-27668762 | None:intergenic | 35.0% | |
| CATAGAAATTGTTGACACAC+AGG | - | chr4.3:27668381-27668400 | None:intergenic | 35.0% | |
| GGGGATTCTCTTTAAATTCA+TGG | - | chr4.3:27668006-27668025 | None:intergenic | 35.0% | |
| TAAAATTTAGGGGCTCTTTC+TGG | + | chr4.3:27667739-27667758 | MS.gene059498:intron | 35.0% | |
| TGTGTGCATGCAATCAAAAT+TGG | + | chr4.3:27668564-27668583 | MS.gene059498:intron | 35.0% | |
| TTAGACACACCTTTAATCTC+AGG | + | chr4.3:27668202-27668221 | MS.gene059498:intron | 35.0% | |
| TTTGATCTAGTCCTCATCAT+AGG | - | chr4.3:27667829-27667848 | None:intergenic | 35.0% | |
| ! | ACTTATGACCCTGTTTGTTA+AGG | + | chr4.3:27668732-27668751 | MS.gene059498:intron | 35.0% |
| ! | CTTATGACCCTGTTTGTTAA+GGG | + | chr4.3:27668733-27668752 | MS.gene059498:intron | 35.0% |
| ! | GTGAAACTCCTCAAAGTATT+TGG | - | chr4.3:27667596-27667615 | None:intergenic | 35.0% |
| !! | GAGGTTGATGGAAACTATTA+TGG | + | chr4.3:27668936-27668955 | MS.gene059498:CDS | 35.0% |
| AGATAAGAAGGCCTATGATG+AGG | + | chr4.3:27667815-27667834 | MS.gene059498:CDS | 40.0% | |
| AGTGGTAGTTTAATCAGCAG+AGG | - | chr4.3:27668611-27668630 | None:intergenic | 40.0% | |
| ATCGACATAGTGAGATTCCA+CGG | - | chr4.3:27669128-27669147 | None:intergenic | 40.0% | |
| CACAGTATCCCTTAACAAAC+AGG | - | chr4.3:27668744-27668763 | None:intergenic | 40.0% | |
| GCTGAATTGAAGCTAGAGTA+TGG | - | chr4.3:27668413-27668432 | None:intergenic | 40.0% | |
| GGCCTTCTTATCTTATCAAG+AGG | - | chr4.3:27667808-27667827 | None:intergenic | 40.0% | |
| GTGTATCCAACTATGTAGCA+CGG | - | chr4.3:27668231-27668250 | None:intergenic | 40.0% | |
| TAATCTTTGTAGCTGAGGGT+TGG | + | chr4.3:27668282-27668301 | MS.gene059498:intron | 40.0% | |
| TGCAGGTTCCAAATACTTTG+AGG | + | chr4.3:27667585-27667604 | MS.gene059498:CDS | 40.0% | |
| TTTCAAGATTCTGCTAACCG+TGG | + | chr4.3:27669108-27669127 | MS.gene059498:intron | 40.0% | |
| AATTTAGGGGCTCTTTCTGG+TGG | + | chr4.3:27667742-27667761 | MS.gene059498:intron | 45.0% | |
| AGAGGAAGTTCCAGTATCAC+TGG | - | chr4.3:27667790-27667809 | None:intergenic | 45.0% | |
| ATTGATGTGCTCGAGGTTGA+TGG | + | chr4.3:27668924-27668943 | MS.gene059498:CDS | 45.0% | |
| CCACCATACGCTGCAAAATA+CGG | + | chr4.3:27669217-27669236 | MS.gene059498:CDS | 45.0% | |
| TCTGCTTCATCCAACCCTAA+TGG | + | chr4.3:27667620-27667639 | MS.gene059498:CDS | 45.0% | |
| ! | ATTCAAAGACCGACTAGTCC+AGG | - | chr4.3:27668464-27668483 | None:intergenic | 45.0% |
| ! | CGTATTTTGCAGCGTATGGT+GGG | - | chr4.3:27669219-27669238 | None:intergenic | 45.0% |
| ! | GTATTTTGCAGCGTATGGTG+GGG | - | chr4.3:27669218-27669237 | None:intergenic | 45.0% |
| ! | TTCAAAGACCGACTAGTCCA+GGG | - | chr4.3:27668463-27668482 | None:intergenic | 45.0% |
| !!! | TAATTATTTTTAAAATTTAG+GGG | + | chr4.3:27667729-27667748 | MS.gene059498:intron | 5.0% |
| ACCAATCCACCGTGCACTAA+CGG | - | chr4.3:27668439-27668458 | None:intergenic | 50.0% | |
| AGGTGTCCGTGCTACATAGT+TGG | + | chr4.3:27668222-27668241 | MS.gene059498:intron | 50.0% | |
| ATACGCTGCAAAATACGGCG+TGG | + | chr4.3:27669222-27669241 | MS.gene059498:CDS | 50.0% | |
| CTTGTGGTCGTAGGTTTCTC+TGG | + | chr4.3:27669156-27669175 | MS.gene059498:intron | 50.0% | |
| GAGTGATGCACCATTAGGGT+TGG | - | chr4.3:27667633-27667652 | None:intergenic | 50.0% | |
| GATGCAGATTGATGTGCTCG+AGG | + | chr4.3:27668917-27668936 | MS.gene059498:intron | 50.0% | |
| GCACGGACACCTGAGATTAA+AGG | - | chr4.3:27668214-27668233 | None:intergenic | 50.0% | |
| TAGGTTTCTCTGGCTGTCAC+CGG | + | chr4.3:27669166-27669185 | MS.gene059498:intron | 50.0% | |
| ! | ACGCCGTATTTTGCAGCGTA+TGG | - | chr4.3:27669223-27669242 | None:intergenic | 50.0% |
| ! | CCGTATTTTGCAGCGTATGG+TGG | - | chr4.3:27669220-27669239 | None:intergenic | 50.0% |
| !! | CTTTCTGGTGGTTCTGGAAG+TGG | + | chr4.3:27667754-27667773 | MS.gene059498:CDS | 50.0% |
| !! | GATTGGTACCCTGGACTAGT+CGG | + | chr4.3:27668452-27668471 | MS.gene059498:intron | 50.0% |
| AATCCACCGTGCACTAACGG+GGG | - | chr4.3:27668436-27668455 | None:intergenic | 55.0% | |
| CAATCCACCGTGCACTAACG+GGG | - | chr4.3:27668437-27668456 | None:intergenic | 55.0% | |
| CCAATCCACCGTGCACTAAC+GGG | - | chr4.3:27668438-27668457 | None:intergenic | 55.0% | |
| TGGTGTGGGTCCAGTGATAC+TGG | + | chr4.3:27667777-27667796 | MS.gene059498:CDS | 55.0% | |
| !! | GGTTCTGGAAGTGGTGGTGT+GGG | + | chr4.3:27667763-27667782 | MS.gene059498:CDS | 55.0% |
| !! | TCTGGTGGTTCTGGAAGTGG+TGG | + | chr4.3:27667757-27667776 | MS.gene059498:CDS | 55.0% |
| !! | TGGTTCTGGAAGTGGTGGTG+TGG | + | chr4.3:27667762-27667781 | MS.gene059498:CDS | 55.0% |
| ATCCACCGTGCACTAACGGG+GGG | - | chr4.3:27668435-27668454 | None:intergenic | 60.0% | |
| CCCGTTAGTGCACGGTGGAT+TGG | + | chr4.3:27668435-27668454 | MS.gene059498:intron | 60.0% | |
| TACGAGGCTCACCAGCGACT+TGG | + | chr4.3:27669189-27669208 | MS.gene059498:CDS | 60.0% | |
| TTCAGCCCCCCGTTAGTGCA+CGG | + | chr4.3:27668427-27668446 | MS.gene059498:intron | 60.0% | |
| ! | GGGGCTCTTTCTGGTGGTTC+TGG | + | chr4.3:27667748-27667767 | MS.gene059498:intron | 60.0% |
| !! | TGCACGGTGGATTGGTACCC+TGG | + | chr4.3:27668443-27668462 | MS.gene059498:intron | 60.0% |
| CTCTGGCTGTCACCGGTACG+AGG | + | chr4.3:27669173-27669192 | MS.gene059498:CDS | 65.0% | |
| GTCGCTGGTGAGCCTCGTAC+CGG | - | chr4.3:27669188-27669207 | None:intergenic | 65.0% | |
| TGGTGGGGAGTCCAAGTCGC+TGG | - | chr4.3:27669203-27669222 | None:intergenic | 65.0% | |
| AGCCCCCCGTTAGTGCACGG+TGG | + | chr4.3:27668430-27668449 | MS.gene059498:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 27667569 | 27669262 | 27667569 | ID=MS.gene059498 |
| chr4.3 | mRNA | 27667569 | 27669262 | 27667569 | ID=MS.gene059498.t1;Parent=MS.gene059498 |
| chr4.3 | exon | 27667569 | 27667641 | 27667569 | ID=MS.gene059498.t1.exon1;Parent=MS.gene059498.t1 |
| chr4.3 | CDS | 27667569 | 27667641 | 27667569 | ID=cds.MS.gene059498.t1;Parent=MS.gene059498.t1 |
| chr4.3 | exon | 27667749 | 27667909 | 27667749 | ID=MS.gene059498.t1.exon2;Parent=MS.gene059498.t1 |
| chr4.3 | CDS | 27667749 | 27667909 | 27667749 | ID=cds.MS.gene059498.t1;Parent=MS.gene059498.t1 |
| chr4.3 | exon | 27668924 | 27668957 | 27668924 | ID=MS.gene059498.t1.exon3;Parent=MS.gene059498.t1 |
| chr4.3 | CDS | 27668924 | 27668957 | 27668924 | ID=cds.MS.gene059498.t1;Parent=MS.gene059498.t1 |
| chr4.3 | exon | 27669169 | 27669262 | 27669169 | ID=MS.gene059498.t1.exon4;Parent=MS.gene059498.t1 |
| chr4.3 | CDS | 27669169 | 27669262 | 27669169 | ID=cds.MS.gene059498.t1;Parent=MS.gene059498.t1 |
| Gene Sequence |
| Protein sequence |
