AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059759

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059759.t1 MTR_2g078620 98.447 322 5 0 1 322 1 322 0.0 651
MS.gene059759.t1 MTR_2g083910 47.059 102 42 5 225 319 134 230 2.54e-18 82.8
MS.gene059759.t1 MTR_2g083910 47.059 102 42 5 225 319 134 230 4.11e-18 82.8
MS.gene059759.t1 MTR_2g016760 39.053 169 83 5 167 320 66 229 3.61e-17 81.6
MS.gene059759.t1 MTR_8g086820 39.669 121 54 4 216 320 123 240 1.20e-16 78.6
MS.gene059759.t1 MTR_4g080340 40.367 109 47 3 229 319 128 236 5.91e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059759.t1 AT3G15115 29.714 350 170 16 18 319 12 333 5.22e-19 86.7

Find 56 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCTTATTTCCCCAATT+TGG 0.135591 2.4:-19942164 None:intergenic
TCTTCTTCTTTCACTCTAAT+TGG 0.176697 2.4:-19940878 None:intergenic
CAACTCCTCCTGTTCCAAAT+TGG 0.187842 2.4:+19942150 MS.gene059759:CDS
CTTAAACAAAGAGGGTATTT+TGG 0.192411 2.4:+19941893 MS.gene059759:CDS
TGGAGGCAAGTTGTCATTAT+TGG 0.201109 2.4:-19941140 None:intergenic
AGTTCAAGGGTTCAAGGATT+TGG 0.219405 2.4:+19941983 MS.gene059759:CDS
GGTGCTCTTTGCAGCTTCTT+AGG 0.254314 2.4:-19940845 None:intergenic
GAATGACACCTTAACATCTT+TGG 0.264564 2.4:-19940734 None:intergenic
CAAGAAACTGATTCAAGAAT+TGG 0.268407 2.4:+19941088 MS.gene059759:CDS
AACTCCTCCTGTTCCAAATT+GGG 0.290556 2.4:+19942151 MS.gene059759:CDS
GTTCAAGGGTTCAAGGATTT+GGG 0.318307 2.4:+19941984 MS.gene059759:CDS
GGAGGCAAGTTGTCATTATT+GGG 0.324183 2.4:-19941139 None:intergenic
CAAGAGCTATTATTATCATT+TGG 0.336403 2.4:-19940623 None:intergenic
ACTCTAATTGGTGGCATAGA+TGG 0.345119 2.4:-19940866 None:intergenic
CCATCTTTGCCTCCAACAAT+AGG 0.347224 2.4:+19941049 MS.gene059759:CDS
AGCTTAGCTAACATAATTCC+TGG 0.370469 2.4:+19942038 MS.gene059759:CDS
GCCTCCAAGGTTAACCTCTA+AGG 0.388438 2.4:+19941156 MS.gene059759:CDS
TCCTCTCTCCCTATTGTTGG+AGG 0.400398 2.4:-19941058 None:intergenic
AGGATGAACAAAATCACTAT+TGG 0.403416 2.4:-19940825 None:intergenic
TCCTAAATCAGAAAACAATA+AGG 0.414045 2.4:+19941931 MS.gene059759:CDS
TTATTTCCCCAATTTGGAAC+AGG 0.427610 2.4:-19942158 None:intergenic
ATATAAAACCAAAGATGTTA+AGG 0.433545 2.4:+19940726 MS.gene059759:CDS
GGAATTATGTTAGCTAAGCT+TGG 0.440726 2.4:-19942035 None:intergenic
TCTTCTTTCACTCTAATTGG+TGG 0.448745 2.4:-19940875 None:intergenic
CCTATTGTTGGAGGCAAAGA+TGG 0.449300 2.4:-19941049 None:intergenic
CTTGAGTATGAAGAAGTTCA+AGG 0.460785 2.4:+19941969 MS.gene059759:CDS
ATAGAAGTGATAGCAATAGA+AGG 0.463972 2.4:+19941005 MS.gene059759:CDS
ATTAGTCTTGTTGAAGGGTA+AGG 0.475567 2.4:-19940761 None:intergenic
CATCTTTGCCTCCAACAATA+GGG 0.480386 2.4:+19941050 MS.gene059759:CDS
ACCTTAGAGGTTAACCTTGG+AGG 0.482914 2.4:-19941157 None:intergenic
GAGCTGATTAGTCTTGTTGA+AGG 0.486253 2.4:-19940767 None:intergenic
ATCGGATTCTGCTATGCATA+AGG 0.492617 2.4:+19940940 MS.gene059759:CDS
TGAAGAAGTTCAAGGGTTCA+AGG 0.522733 2.4:+19941977 MS.gene059759:CDS
AGTACCTTAGAGGTTAACCT+TGG 0.525551 2.4:-19941160 None:intergenic
AACCATGCCTCTGATAAGTA+AGG 0.533103 2.4:-19942113 None:intergenic
TCATTATTGGGAGGTGTCGA+TGG 0.539813 2.4:-19941127 None:intergenic
ATAATGACAACTTGCCTCCA+AGG 0.544515 2.4:+19941143 MS.gene059759:CDS
TGAATCTACTTGAAGAGTGT+TGG 0.552194 2.4:+19940684 MS.gene059759:CDS
TTACTTATCAGAGGCATGGT+TGG 0.560586 2.4:+19942115 MS.gene059759:CDS
TGAACAAAATCACTATTGGA+TGG 0.561406 2.4:-19940821 None:intergenic
CATCATTGTGCAAAGATGAA+AGG 0.561895 2.4:+19940974 MS.gene059759:CDS
ACTCCTCCTGTTCCAAATTG+GGG 0.566945 2.4:+19942152 MS.gene059759:CDS
CTTCATACTCAAGATCACTT+AGG 0.571003 2.4:-19941959 None:intergenic
GATTCTGCTATGCATAAGGC+AGG 0.572587 2.4:+19940944 MS.gene059759:CDS
CAGCATTCCAAGAAGTAGAC+CGG 0.572646 2.4:+19941848 MS.gene059759:CDS
GACCTTACTTATCAGAGGCA+TGG 0.573969 2.4:+19942111 MS.gene059759:CDS
GTTGAAAGCTTAAACAAAGA+GGG 0.576048 2.4:+19941885 MS.gene059759:CDS
AGCTGATTAGTCTTGTTGAA+GGG 0.577448 2.4:-19940766 None:intergenic
TTTCCCCAATTTGGAACAGG+AGG 0.577483 2.4:-19942155 None:intergenic
GCCTCCAACAATAGGGAGAG+AGG 0.599344 2.4:+19941057 MS.gene059759:CDS
AGTTGAAAGCTTAAACAAAG+AGG 0.608171 2.4:+19941884 MS.gene059759:CDS
TTGAGTATGAAGAAGTTCAA+GGG 0.610506 2.4:+19941970 MS.gene059759:CDS
GAGGAAGATAAAGCAGCAAG+AGG 0.618815 2.4:+19942089 MS.gene059759:CDS
CAAATTGGTGCATCAAACAT+CGG 0.624498 2.4:+19940922 MS.gene059759:CDS
GGCAAGTTGTCATTATTGGG+AGG 0.626871 2.4:-19941136 None:intergenic
AAGAGGACCTTACTTATCAG+AGG 0.645280 2.4:+19942106 MS.gene059759:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAAATGCTAAAATTATT+TGG - chr2.4:19941490-19941509 None:intergenic 15.0%
!! AACACCAATTAATACAAATA+TGG + chr2.4:19941456-19941475 MS.gene059759:intron 20.0%
!! ATATAAAACCAAAGATGTTA+AGG + chr2.4:19940726-19940745 MS.gene059759:CDS 20.0%
!! ATGATCAATCAAAATATGAT+TGG - chr2.4:19941660-19941679 None:intergenic 20.0%
!! GAAGAAAAAGATGATAAAAA+AGG + chr2.4:19940896-19940915 MS.gene059759:CDS 20.0%
!! GACATAATGATTTAAAAAGA+GGG - chr2.4:19941389-19941408 None:intergenic 20.0%
!! TAATATCAATCATAAACTCT+TGG - chr2.4:19941416-19941435 None:intergenic 20.0%
!! TGACATAATGATTTAAAAAG+AGG - chr2.4:19941390-19941409 None:intergenic 20.0%
!! TTAATACAAATATGGCTAAA+AGG + chr2.4:19941464-19941483 MS.gene059759:intron 20.0%
!! TTAGCCATATTTGTATTAAT+TGG - chr2.4:19941463-19941482 None:intergenic 20.0%
!!! GAAAAAACATATCAAGTTTT+GGG + chr2.4:19942199-19942218 MS.gene059759:CDS 20.0%
!!! TGAAAAAACATATCAAGTTT+TGG + chr2.4:19942198-19942217 MS.gene059759:CDS 20.0%
! ATAGCATCAGAAATAGAAAA+AGG + chr2.4:19941516-19941535 MS.gene059759:intron 25.0%
! ATATTTGAACATAGCATGAT+AGG - chr2.4:19941567-19941586 None:intergenic 25.0%
! CAAGAGCTATTATTATCATT+TGG - chr2.4:19940626-19940645 None:intergenic 25.0%
! CGAAGAATTGTGATTTAATA+TGG + chr2.4:19941292-19941311 MS.gene059759:intron 25.0%
! TAATGATTTAAAAAGAGGGT+AGG - chr2.4:19941385-19941404 None:intergenic 25.0%
! TCCTAAATCAGAAAACAATA+AGG + chr2.4:19941931-19941950 MS.gene059759:CDS 25.0%
! TGATAAAAAAGGAAGCAAAT+TGG + chr2.4:19940907-19940926 MS.gene059759:CDS 25.0%
!! TCCTTATTGTTTTCTGATTT+AGG - chr2.4:19941935-19941954 None:intergenic 25.0%
!! TGATTTTTATAGTACCTTAG+AGG - chr2.4:19941173-19941192 None:intergenic 25.0%
!! TTTTTGCAAAGATGAAAAAG+TGG + chr2.4:19940655-19940674 MS.gene059759:CDS 25.0%
!!! TATTAATTGGTGTTACTTCT+TGG - chr2.4:19941450-19941469 None:intergenic 25.0%
AATATCGCTTATTGTTACGA+AGG - chr2.4:19941736-19941755 None:intergenic 30.0%
ACAAAAAAAGAGAAGAATGC+AGG + chr2.4:19941618-19941637 MS.gene059759:intron 30.0%
AGGATGAACAAAATCACTAT+TGG - chr2.4:19940828-19940847 None:intergenic 30.0%
AGTTGAAAGCTTAAACAAAG+AGG + chr2.4:19941884-19941903 MS.gene059759:CDS 30.0%
ATAGAAGTGATAGCAATAGA+AGG + chr2.4:19941005-19941024 MS.gene059759:CDS 30.0%
ATGCAACAATAATACGTGAA+AGG - chr2.4:19941702-19941721 None:intergenic 30.0%
ATTTAATATGGCAAGTAGTC+TGG + chr2.4:19941304-19941323 MS.gene059759:intron 30.0%
GAAAAATAGAGATGAAAGTG+AGG + chr2.4:19942070-19942089 MS.gene059759:CDS 30.0%
GTTGAAAGCTTAAACAAAGA+GGG + chr2.4:19941885-19941904 MS.gene059759:CDS 30.0%
TCTTCTTCTTTCACTCTAAT+TGG - chr2.4:19940881-19940900 None:intergenic 30.0%
TGAACAAAATCACTATTGGA+TGG - chr2.4:19940824-19940843 None:intergenic 30.0%
TTGAGTATGAAGAAGTTCAA+GGG + chr2.4:19941970-19941989 MS.gene059759:CDS 30.0%
! CAAGAAACTGATTCAAGAAT+TGG + chr2.4:19941088-19941107 MS.gene059759:CDS 30.0%
!! CTATTTTTCTCTTGTAAACC+AGG - chr2.4:19942059-19942078 None:intergenic 30.0%
!!! CTTAAACAAAGAGGGTATTT+TGG + chr2.4:19941893-19941912 MS.gene059759:CDS 30.0%
AGAGAAGAATGCAGGAATAT+AGG + chr2.4:19941626-19941645 MS.gene059759:intron 35.0%
AGCTTAGCTAACATAATTCC+TGG + chr2.4:19942038-19942057 MS.gene059759:CDS 35.0%
CAAATTGGTGCATCAAACAT+CGG + chr2.4:19940922-19940941 MS.gene059759:CDS 35.0%
CATCATTGTGCAAAGATGAA+AGG + chr2.4:19940974-19940993 MS.gene059759:CDS 35.0%
CTTCATACTCAAGATCACTT+AGG - chr2.4:19941962-19941981 None:intergenic 35.0%
CTTGAGTATGAAGAAGTTCA+AGG + chr2.4:19941969-19941988 MS.gene059759:CDS 35.0%
GAATGACACCTTAACATCTT+TGG - chr2.4:19940737-19940756 None:intergenic 35.0%
GGAATTATGTTAGCTAAGCT+TGG - chr2.4:19942038-19942057 None:intergenic 35.0%
TCTTCTTTCACTCTAATTGG+TGG - chr2.4:19940878-19940897 None:intergenic 35.0%
! AATCAGCTCAGAACTTTTGA+AGG + chr2.4:19940781-19940800 MS.gene059759:CDS 35.0%
! GACATCTTATTTCCCCAATT+TGG - chr2.4:19942167-19942186 None:intergenic 35.0%
! TGAATCTACTTGAAGAGTGT+TGG + chr2.4:19940684-19940703 MS.gene059759:CDS 35.0%
! TTATTTCCCCAATTTGGAAC+AGG - chr2.4:19942161-19942180 None:intergenic 35.0%
!! AGCTGATTAGTCTTGTTGAA+GGG - chr2.4:19940769-19940788 None:intergenic 35.0%
!! ATTAGTCTTGTTGAAGGGTA+AGG - chr2.4:19940764-19940783 None:intergenic 35.0%
!! TATTTTGGAGATGAGGAGAA+AGG + chr2.4:19941908-19941927 MS.gene059759:CDS 35.0%
!!! AACATATCAAGTTTTGGGCT+AGG + chr2.4:19942204-19942223 MS.gene059759:CDS 35.0%
!!! ACATATCAAGTTTTGGGCTA+GGG + chr2.4:19942205-19942224 MS.gene059759:CDS 35.0%
!!! TCAACTTCGATGTTTTTAGC+CGG - chr2.4:19941870-19941889 None:intergenic 35.0%
AACCATGCCTCTGATAAGTA+AGG - chr2.4:19942116-19942135 None:intergenic 40.0%
AACTCCTCCTGTTCCAAATT+GGG + chr2.4:19942151-19942170 MS.gene059759:CDS 40.0%
AAGAGGACCTTACTTATCAG+AGG + chr2.4:19942106-19942125 MS.gene059759:CDS 40.0%
AGTACCTTAGAGGTTAACCT+TGG - chr2.4:19941163-19941182 None:intergenic 40.0%
AGTTCAAGGGTTCAAGGATT+TGG + chr2.4:19941983-19942002 MS.gene059759:CDS 40.0%
ATAATGACAACTTGCCTCCA+AGG + chr2.4:19941143-19941162 MS.gene059759:CDS 40.0%
ATATGGCAAGTAGTCTGGTA+CGG + chr2.4:19941309-19941328 MS.gene059759:intron 40.0%
ATCGGATTCTGCTATGCATA+AGG + chr2.4:19940940-19940959 MS.gene059759:CDS 40.0%
CATCTTTGCCTCCAACAATA+GGG + chr2.4:19941050-19941069 MS.gene059759:CDS 40.0%
GGAGGCAAGTTGTCATTATT+GGG - chr2.4:19941142-19941161 None:intergenic 40.0%
GTTCAAGGGTTCAAGGATTT+GGG + chr2.4:19941984-19942003 MS.gene059759:CDS 40.0%
TCGTTTATGATTAAGCGCGT+AGG + chr2.4:19941236-19941255 MS.gene059759:intron 40.0%
TGAAGAAGTTCAAGGGTTCA+AGG + chr2.4:19941977-19941996 MS.gene059759:CDS 40.0%
TGGAGGCAAGTTGTCATTAT+TGG - chr2.4:19941143-19941162 None:intergenic 40.0%
! ACTCTAATTGGTGGCATAGA+TGG - chr2.4:19940869-19940888 None:intergenic 40.0%
! TTACTTATCAGAGGCATGGT+TGG + chr2.4:19942115-19942134 MS.gene059759:CDS 40.0%
! TTTTCCTCTCTCCCTATTGT+TGG - chr2.4:19941064-19941083 None:intergenic 40.0%
!! GAGCTGATTAGTCTTGTTGA+AGG - chr2.4:19940770-19940789 None:intergenic 40.0%
!! TTTTTAGCCGGTCTACTTCT+TGG - chr2.4:19941858-19941877 None:intergenic 40.0%
!!! AAGAGGGTATTTTGGAGATG+AGG + chr2.4:19941901-19941920 MS.gene059759:CDS 40.0%
ACCTTAGAGGTTAACCTTGG+AGG - chr2.4:19941160-19941179 None:intergenic 45.0%
ACTCCTCCTGTTCCAAATTG+GGG + chr2.4:19942152-19942171 MS.gene059759:CDS 45.0%
CAACTCCTCCTGTTCCAAAT+TGG + chr2.4:19942150-19942169 MS.gene059759:CDS 45.0%
CAGCATTCCAAGAAGTAGAC+CGG + chr2.4:19941848-19941867 MS.gene059759:CDS 45.0%
CCATCTTTGCCTCCAACAAT+AGG + chr2.4:19941049-19941068 MS.gene059759:CDS 45.0%
CCTATTGTTGGAGGCAAAGA+TGG - chr2.4:19941052-19941071 None:intergenic 45.0%
GACCTTACTTATCAGAGGCA+TGG + chr2.4:19942111-19942130 MS.gene059759:CDS 45.0%
GAGGAAGATAAAGCAGCAAG+AGG + chr2.4:19942089-19942108 MS.gene059759:CDS 45.0%
GATTCTGCTATGCATAAGGC+AGG + chr2.4:19940944-19940963 MS.gene059759:CDS 45.0%
GGCAAGTTGTCATTATTGGG+AGG - chr2.4:19941139-19941158 None:intergenic 45.0%
! TTTCCCCAATTTGGAACAGG+AGG - chr2.4:19942158-19942177 None:intergenic 45.0%
!! TCATTATTGGGAGGTGTCGA+TGG - chr2.4:19941130-19941149 None:intergenic 45.0%
!! TGGTGTTACTTCTTGGTGCT+AGG - chr2.4:19941443-19941462 None:intergenic 45.0%
!!! AGGTGTCGATGGTTGTTTTC+GGG - chr2.4:19941119-19941138 None:intergenic 45.0%
!!! GAGGTGTCGATGGTTGTTTT+CGG - chr2.4:19941120-19941139 None:intergenic 45.0%
AACTGCAACGTGACCGTGAT+TGG - chr2.4:19941337-19941356 None:intergenic 50.0%
GCCTCCAAGGTTAACCTCTA+AGG + chr2.4:19941156-19941175 MS.gene059759:CDS 50.0%
GGTGCTCTTTGCAGCTTCTT+AGG - chr2.4:19940848-19940867 None:intergenic 50.0%
GTCTGGTACGGTTCCAATCA+CGG + chr2.4:19941321-19941340 MS.gene059759:intron 50.0%
TCCTCTCTCCCTATTGTTGG+AGG - chr2.4:19941061-19941080 None:intergenic 50.0%
CACGGTCACGTTGCAGTTAC+AGG + chr2.4:19941339-19941358 MS.gene059759:intron 55.0%
GCCTCCAACAATAGGGAGAG+AGG + chr2.4:19941057-19941076 MS.gene059759:CDS 55.0%
!!! CAAGTTTTGGGCTAGGGCTG+TGG + chr2.4:19942211-19942230 MS.gene059759:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 19940617 19942250 19940617 ID=MS.gene059759
chr2.4 mRNA 19940617 19942250 19940617 ID=MS.gene059759.t1;Parent=MS.gene059759
chr2.4 exon 19940617 19941177 19940617 ID=MS.gene059759.t1.exon1;Parent=MS.gene059759.t1
chr2.4 CDS 19940617 19941177 19940617 ID=cds.MS.gene059759.t1;Parent=MS.gene059759.t1
chr2.4 exon 19941843 19942250 19941843 ID=MS.gene059759.t1.exon2;Parent=MS.gene059759.t1
chr2.4 CDS 19941843 19942250 19941843 ID=cds.MS.gene059759.t1;Parent=MS.gene059759.t1
Gene Sequence

>MS.gene059759

ATGGATCCAAATGATAATAATAGCTCTTGTGAATCTTCTTTTTGCAAAGATGAAAAAGTGGAAGCAATGAATCTACTTGAAGAGTGTTGGTATTTTGATAACTTGTTAAATATAAAACCAAAGATGTTAAGGTGTCATTCTGATCCTTACCCTTCAACAAGACTAATCAGCTCAGAACTTTTGAAGGATTCATCAGCAAGTAAACCATCCAATAGTGATTTTGTTCATCCTAAGAAGCTGCAAAGAGCACCATCTATGCCACCAATTAGAGTGAAAGAAGAAGAAAAAGATGATAAAAAAGGAAGCAAATTGGTGCATCAAACATCGGATTCTGCTATGCATAAGGCAGGCAGTAAACATCATTGTGCAAAGATGAAAGGACTTGATCATAGAAGTGATAGCAATAGAAGGAAAAGCAAATTGCTAAGAACACCATCTTTGCCTCCAACAATAGGGAGAGAGGAAAAGTTTCAAGAAACTGATTCAAGAATTGGAAGATCCCGAAAACAACCATCGACACCTCCCAATAATGACAACTTGCCTCCAAGGTTAACCTCTAAGGTACTATAAAAATCATCTTGCTTTTATAAAGATCTAAGTAATGTATAGACTTTTTTTTTCGTTTATGATTAAGCGCGTAGGTTTTAAATTGTGATTTGTCGTGCTTCAATATTGCGAAGAATTGTGATTTAATATGGCAAGTAGTCTGGTACGGTTCCAATCACGGTCACGTTGCAGTTACAGGAAATGAAATACATTATGTCGCCTACCCTCTTTTTAAATCATTATGTCACATCCAAGAGTTTATGATTGATATTATGTGCCTAGCACCAAGAAGTAACACCAATTAATACAAATATGGCTAAAAGGCCAAATAATTTTAGCATTTAAGTAAAATCATAGCATCAGAAATAGAAAAAGGCAACATTTTAAGAAAAGTCATTAGTCCTATCATGCTATGTTCAAATATAGAGCAATGTTATTTCTTACAAATGATTTTTACAAAAAAAGAGAAGAATGCAGGAATATAGGATATTTAACCAATCATATTTTGATTGATCATACGCGAGAAAGCGTCACTTCCTTTCACGTATTATTGTTGCATTTCTTGATACTCCTTCGTAACAATAAGCGATATTCTTGAATATAAGTACTTCAAATTTCAAATTGATTTGAATGTTTATGAAGCGAACTCATTGTATATGTTTTGCAATGCTTGATGACAGAGTTGCAGCATTCCAAGAAGTAGACCGGCTAAAAACATCGAAGTTGAAAGCTTAAACAAAGAGGGTATTTTGGAGATGAGGAGAAAGGTCCTAAATCAGAAAACAATAAGGAGAAGCCTAAGTGATCTTGAGTATGAAGAAGTTCAAGGGTTCAAGGATTTGGGATTCTCATTTGAGAAAGAAGCTTTAAGTCCAAGCTTAGCTAACATAATTCCTGGTTTACAAGAGAAAAATAGAGATGAAAGTGAGGAAGATAAAGCAGCAAGAGGACCTTACTTATCAGAGGCATGGTTGGTGCAAAGTTGTGCAACTCCTCCTGTTCCAAATTGGGGAAATAAGATGTCTGCAGATGACATGAAAAAACATATCAAGTTTTGGGCTAGGGCTGTGGCTTCAACTAAATGTTGA
Protein sequence

>MS.gene059759.t1

MDPNDNNSSCESSFCKDEKVEAMNLLEECWYFDNLLNIKPKMLRCHSDPYPSTRLISSELLKDSSASKPSNSDFVHPKKLQRAPSMPPIRVKEEEKDDKKGSKLVHQTSDSAMHKAGSKHHCAKMKGLDHRSDSNRRKSKLLRTPSLPPTIGREEKFQETDSRIGRSRKQPSTPPNNDNLPPRLTSKSCSIPRSRPAKNIEVESLNKEGILEMRRKVLNQKTIRRSLSDLEYEEVQGFKDLGFSFEKEALSPSLANIIPGLQEKNRDESEEDKAARGPYLSEAWLVQSCATPPVPNWGNKMSADDMKKHIKFWARAVASTKC