AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060581.t1 MTR_2g076210 95.095 367 18 0 1 367 1 367 0.0 737
MS.gene060581.t1 MTR_2g076200 74.731 372 85 5 1 367 4 371 0.0 563
MS.gene060581.t1 MTR_2g020280 48.056 360 153 8 37 367 39 393 2.69e-106 318
MS.gene060581.t1 MTR_5g020580 47.541 366 153 10 37 367 55 416 1.45e-103 311
MS.gene060581.t1 MTR_8g077195 46.277 376 165 7 25 367 41 412 1.66e-101 306
MS.gene060581.t1 MTR_1g088960 46.814 361 154 9 37 367 50 402 2.15e-97 295
MS.gene060581.t1 MTR_1g098790 44.648 383 156 10 37 367 53 431 2.11e-96 293
MS.gene060581.t1 MTR_1g022370 47.472 356 159 12 37 367 70 422 7.75e-95 289
MS.gene060581.t1 MTR_2g007930 43.158 380 161 11 37 367 54 427 1.15e-93 286
MS.gene060581.t1 MTR_2g020280 47.826 299 122 8 98 367 1 294 6.35e-80 247
MS.gene060581.t1 MTR_8g077620 43.258 356 173 10 25 360 40 386 9.75e-75 236
MS.gene060581.t1 MTR_8g077675 37.568 370 187 9 25 360 36 395 8.59e-67 216
MS.gene060581.t1 MTR_8g077640 39.067 343 139 11 34 357 92 383 5.63e-48 167
MS.gene060581.t1 MTR_8g077650 35.359 362 179 13 19 359 1 328 1.13e-44 157
MS.gene060581.t1 MTR_1g023800 50.000 98 49 0 35 132 50 147 1.54e-26 103
MS.gene060581.t1 MTR_0210s0010 37.209 86 48 1 80 165 9 88 7.72e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060581.t1 AT1G53320 62.240 384 123 7 1 367 1 379 5.68e-158 448
MS.gene060581.t1 AT2G47900 44.444 360 166 6 37 367 52 406 5.88e-102 307
MS.gene060581.t1 AT2G47900 44.444 360 166 6 37 367 52 406 5.88e-102 307
MS.gene060581.t1 AT2G47900 44.321 361 166 6 37 367 52 407 1.15e-101 306
MS.gene060581.t1 AT2G18280 46.307 352 168 5 34 367 45 393 2.34e-100 302
MS.gene060581.t1 AT2G18280 46.307 352 168 5 34 367 45 393 2.34e-100 302
MS.gene060581.t1 AT1G76900 40.371 431 173 9 18 367 28 455 1.08e-96 295
MS.gene060581.t1 AT1G76900 40.371 431 173 9 18 367 28 455 1.08e-96 295
MS.gene060581.t1 AT1G76900 40.371 431 173 9 18 367 28 455 1.08e-96 295
MS.gene060581.t1 AT3G06380 45.304 362 165 9 31 367 27 380 4.29e-95 288
MS.gene060581.t1 AT1G25280 43.734 391 156 11 37 367 59 445 3.31e-94 288
MS.gene060581.t1 AT5G18680 43.700 373 169 10 25 367 28 389 1.04e-88 273
MS.gene060581.t1 AT1G47270 43.978 357 170 10 33 367 40 388 2.09e-88 271
MS.gene060581.t1 AT1G47270 43.978 357 170 10 33 367 65 413 3.00e-88 272
MS.gene060581.t1 AT1G47270 43.978 357 170 10 33 367 64 412 3.39e-88 272
MS.gene060581.t1 AT1G43640 41.253 383 169 10 35 367 53 429 3.48e-87 270
MS.gene060581.t1 AT1G43640 41.463 369 160 10 49 367 1 363 1.56e-83 258
MS.gene060581.t1 AT3G06380 43.836 292 132 8 100 367 5 288 3.13e-68 216
MS.gene060581.t1 AT5G18680 43.144 299 131 8 98 367 9 297 1.42e-65 210
MS.gene060581.t1 AT1G25280 40.590 271 98 10 156 367 1 267 4.32e-50 169
MS.gene060581.t1 AT1G25280 40.590 271 98 10 156 367 1 267 4.32e-50 169
MS.gene060581.t1 AT1G61940 48.276 87 45 0 40 126 1 87 1.15e-22 96.3
MS.gene060581.t1 AT1G61940 49.180 61 31 0 40 100 1 61 9.80e-15 73.6

Find 91 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCACCACTGTGTTGTAAAT+TGG 0.184549 2.2:-17647858 MS.gene060581:CDS
ATCATTCGCCTTGGACGTTT+TGG 0.253223 2.2:+17647094 None:intergenic
ATAATATCCGCAAGAAGTTC+TGG 0.306087 2.2:+17648872 None:intergenic
GCCATTTGCTTAACTAGCTT+TGG 0.307225 2.2:-17646722 MS.gene060581:CDS
TGGGGAAGGTCGCGTGGCCC+TGG 0.326133 2.2:+17647877 None:intergenic
CGGCTTGTTCTGTCACTTGT+TGG 0.336212 2.2:+17647208 None:intergenic
TGTTCTGTCACTTGTTGGTT+TGG 0.343539 2.2:+17647213 None:intergenic
TTTCCCTTCTTGTCTTAAAC+AGG 0.347974 2.2:-17648717 MS.gene060581:intron
GATATCTTCTAGCTGCTAAT+AGG 0.354984 2.2:+17647608 None:intergenic
TCTTCATCTCCTCTCCCTCC+TGG 0.374907 2.2:+17646830 None:intergenic
GCTATCTGAACTTTCGCATA+TGG 0.381471 2.2:+17647045 None:intergenic
ATCTTACTGACAAGCCAGTT+TGG 0.393584 2.2:+17646697 None:intergenic
TCGCCAGCATTAACAGATAC+AGG 0.396379 2.2:-17647637 MS.gene060581:intron
AACTAGCTTTGGCACCAAAC+TGG 0.411927 2.2:-17646711 MS.gene060581:CDS
GTACTTACTTACATGGTGTA+AGG 0.424832 2.2:+17647800 None:intergenic
CAAAGCCAACCAGGAGGGAG+AGG 0.425267 2.2:-17646839 MS.gene060581:CDS
CTGTCATATCCTTTCTCTCA+TGG 0.452722 2.2:+17646903 None:intergenic
GTAGGGTCACCTTCTCTTAA+CGG 0.460650 2.2:-17648749 MS.gene060581:CDS
ACAATTTATGACGCCTCGGC+TGG 0.460766 2.2:-17647238 MS.gene060581:CDS
CTTGAACTTCCATGAGAGAA+AGG 0.465557 2.2:-17646912 MS.gene060581:CDS
TCCGATGTGGAACGAGCAAT+TGG 0.466061 2.2:-17646945 MS.gene060581:CDS
CCTGCTGGTAACGTCGAGGC+AGG 0.472577 2.2:-17647151 MS.gene060581:CDS
CAAGGAAGTAATGCTTATGT+TGG 0.475540 2.2:-17647532 MS.gene060581:CDS
AAACGCATGGAAGGCAGATA+AGG 0.478629 2.2:+17646745 None:intergenic
CATCTCCTCTCCCTCCTGGT+TGG 0.480773 2.2:+17646834 None:intergenic
TATTAGCAGCTAGAAGATAT+CGG 0.489255 2.2:-17647606 MS.gene060581:CDS
CTTGTGCCTGCGTTTGCAAA+CGG 0.489375 2.2:-17648796 MS.gene060581:CDS
CGTTACCAGCAGGAACTTGA+GGG 0.494917 2.2:+17647161 None:intergenic
CTTTCAGCTGGTTGCTACTG+TGG 0.494990 2.2:-17646864 MS.gene060581:CDS
TTTGTCCTCCGATGCACTAC+CGG 0.499562 2.2:+17648916 None:intergenic
AACGCATGGAAGGCAGATAA+GGG 0.500964 2.2:+17646746 None:intergenic
CCAATTTACAACACAGTGGT+GGG 0.506056 2.2:+17647858 None:intergenic
GCCAAAGCTAGTTAAGCAAA+TGG 0.506693 2.2:+17646721 None:intergenic
TGTTGGTTTGGCACCAGCCG+AGG 0.517892 2.2:+17647225 None:intergenic
AGAGAGCAAATCATTCGCCT+TGG 0.519881 2.2:+17647085 None:intergenic
CAGATACTTCAACAATGCTC+AGG 0.525873 2.2:-17648966 MS.gene060581:CDS
ATCAGTGAAGAACTTTCAGC+TGG 0.527624 2.2:-17646876 MS.gene060581:CDS
ATTAAACGAGTTGATGTCAC+CGG 0.529161 2.2:-17648851 MS.gene060581:CDS
ATGCTTATGTTGGCAAGCTA+AGG 0.535171 2.2:-17647522 MS.gene060581:intron
TCCAATTTACAACACAGTGG+TGG 0.539488 2.2:+17647857 None:intergenic
CATCGGAGGACAAAACTGAT+TGG 0.540142 2.2:-17648907 MS.gene060581:CDS
ACGTTACCAGCAGGAACTTG+AGG 0.541472 2.2:+17647160 None:intergenic
ACGATGCGGCCATTGTTCCC+CGG 0.542118 2.2:+17648832 None:intergenic
AGGACCTGTTTAAGACAAGA+AGG 0.544045 2.2:+17648713 None:intergenic
CGCAAGAAGTTCTGGAAGCA+TGG 0.545732 2.2:+17648880 None:intergenic
ATAAGCCCTCAAGTTCCTGC+TGG 0.547760 2.2:-17647166 MS.gene060581:CDS
TGAACTTTCGCATATGGATG+AGG 0.550314 2.2:+17647051 None:intergenic
ATATCACACGTGAGGTCGTA+GGG 0.551715 2.2:-17648766 MS.gene060581:CDS
CTTTCGCATATGGATGAGGC+AGG 0.555383 2.2:+17647055 None:intergenic
CTTCACAATTTATGACGCCT+CGG 0.560452 2.2:-17647242 MS.gene060581:CDS
TTGATGTCACCGGGGAACAA+TGG 0.560459 2.2:-17648841 MS.gene060581:CDS
GGCAAGATAGAGGTAAAATG+TGG 0.562187 2.2:+17647822 None:intergenic
ACTTCCTTGTGAAAAGTCAT+CGG 0.574248 2.2:+17647546 None:intergenic
TTTATTCTTCAAGATTGTGT+AGG 0.575970 2.2:+17646970 None:intergenic
GGAACGAGCAATTGGAGTGC+TGG 0.578985 2.2:-17646937 MS.gene060581:CDS
GCAAATGGCAAACGCATGGA+AGG 0.579450 2.2:+17646736 None:intergenic
CAGTGGTGGGGAAGGTCGCG+TGG 0.579862 2.2:+17647871 None:intergenic
TTAAACGAGTTGATGTCACC+GGG 0.582848 2.2:-17648850 MS.gene060581:CDS
CCTGCCTCGACGTTACCAGC+AGG 0.584116 2.2:+17647151 None:intergenic
CGCCAGCATTAACAGATACA+GGG 0.585383 2.2:-17647636 MS.gene060581:intron
TACTTACTTACATGGTGTAA+GGG 0.588591 2.2:+17647801 None:intergenic
GATATCACACGTGAGGTCGT+AGG 0.591293 2.2:-17648767 MS.gene060581:CDS
TTAAGCAAATGGCAAACGCA+TGG 0.595995 2.2:+17646732 None:intergenic
ACTGTGGATCAAAGCCAACC+AGG 0.600528 2.2:-17646848 MS.gene060581:CDS
TCTGTTTGCTTGCAAAACGC+CGG 0.603910 2.2:+17647188 None:intergenic
TAAGGGCTGTCTATAGTACA+TGG 0.607232 2.2:+17646763 None:intergenic
CACCACCGGTAGTGCATCGG+AGG 0.607308 2.2:-17648921 MS.gene060581:CDS
TGACACCACCGGTAGTGCAT+CGG 0.608180 2.2:-17648924 MS.gene060581:CDS
GTGCCTGCGTTTGCAAACGG+TGG 0.610120 2.2:-17648793 MS.gene060581:CDS
TTACAACACAGTGGTGGGGA+AGG 0.615695 2.2:+17647863 None:intergenic
ATGGTGTAAGGGCAAGATAG+AGG 0.623484 2.2:+17647812 None:intergenic
CATGCTTCCAGAACTTCTTG+CGG 0.624235 2.2:-17648879 MS.gene060581:CDS
CAATTTACAACACAGTGGTG+GGG 0.625248 2.2:+17647859 None:intergenic
TCCAATTGCTCGTTCCACAT+CGG 0.629514 2.2:+17646944 None:intergenic
GTCCTCCGATGCACTACCGG+TGG 0.634176 2.2:+17648919 None:intergenic
TGAAGAATAAAGCTCCGATG+TGG 0.636117 2.2:-17646958 MS.gene060581:CDS
TCTCCACCGTTTGCAAACGC+AGG 0.641600 2.2:+17648790 None:intergenic
GCTCAGGCTCTCAATGCTAC+CGG 0.643110 2.2:-17648950 MS.gene060581:CDS
GGACCTGTTTAAGACAAGAA+GGG 0.643301 2.2:+17648714 None:intergenic
GCTACCGGCGATGACACCAC+CGG 0.645253 2.2:-17648935 MS.gene060581:CDS
ACTACCGGTGGTGTCATCGC+CGG 0.646189 2.2:+17648931 None:intergenic
CTGCTGGTAACGTCGAGGCA+GGG 0.646476 2.2:-17647150 MS.gene060581:CDS
TGGATCAAAGCCAACCAGGA+GGG 0.647654 2.2:-17646844 MS.gene060581:CDS
CAACAAGTGACAGAACAAGC+CGG 0.658598 2.2:-17647207 MS.gene060581:CDS
AAGATAGAGGTAAAATGTGG+AGG 0.660216 2.2:+17647825 None:intergenic
GCGACGACATTTCGACGATG+CGG 0.691215 2.2:+17648818 None:intergenic
TATTCCAATTTACAACACAG+TGG 0.695058 2.2:+17647854 None:intergenic
GTGGAGAGATATCACACGTG+AGG 0.702332 2.2:-17648774 MS.gene060581:CDS
AGTTCCTGCTGGTAACGTCG+AGG 0.707570 2.2:-17647155 MS.gene060581:CDS
GTGGATCAAAGCCAACCAGG+AGG 0.711734 2.2:-17646845 MS.gene060581:CDS
TAAACGAGTTGATGTCACCG+GGG 0.773553 2.2:-17648849 MS.gene060581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATTAAAATTTCAAA+TGG - chr2.2:17647953-17647972 MS.gene060581:intron 10.0%
!! TTGAGAATAAATTCAATAAA+TGG + chr2.2:17648356-17648375 None:intergenic 15.0%
!! ATTTATTGAAGAAAAAGAGT+AGG - chr2.2:17648250-17648269 MS.gene060581:intron 20.0%
!! GATGCATTTATTAAGTATAT+AGG - chr2.2:17647677-17647696 MS.gene060581:intron 20.0%
!! TCAAACTATTAAAGAAGAAA+AGG + chr2.2:17647605-17647624 None:intergenic 20.0%
!! TTAAAATTTCAAATGGAGAT+TGG - chr2.2:17647960-17647979 MS.gene060581:intron 20.0%
!! TTTATGGATCATGAAAAATT+TGG - chr2.2:17647045-17647064 MS.gene060581:CDS 20.0%
!! TTTCAAATTATTAGTTGTGT+CGG + chr2.2:17647180-17647199 None:intergenic 20.0%
!! TTTCTCTCTTGAATAATTTA+TGG - chr2.2:17647029-17647048 MS.gene060581:CDS 20.0%
!!! ATCTTGTTATGTTTTGATAA+AGG - chr2.2:17647369-17647388 MS.gene060581:intron 20.0%
!!! TACTCTTTTTCTTCAATAAA+TGG + chr2.2:17648251-17648270 None:intergenic 20.0%
! AAATAAAATGATCCTCATTC+AGG - chr2.2:17648216-17648235 MS.gene060581:intron 25.0%
! ACAAACTAACTAATAACATC+TGG - chr2.2:17648318-17648337 MS.gene060581:intron 25.0%
! ATCAATTGAATTAAGAGAGT+TGG - chr2.2:17647337-17647356 MS.gene060581:intron 25.0%
! TTTATTCTTCAAGATTGTGT+AGG + chr2.2:17648728-17648747 None:intergenic 25.0%
!! AAATGAATTCATTTTGTTGC+AGG + chr2.2:17647005-17647024 None:intergenic 25.0%
!! CTAACATTTTGTTCAATTAG+TGG - chr2.2:17647103-17647122 MS.gene060581:CDS 25.0%
CAAAACAGTACTTACTTACA+TGG + chr2.2:17647905-17647924 None:intergenic 30.0%
GAAAATGAAACCAAGTTACT+CGG - chr2.2:17647481-17647500 MS.gene060581:intron 30.0%
TACTTACTTACATGGTGTAA+GGG + chr2.2:17647897-17647916 None:intergenic 30.0%
TATTAGCAGCTAGAAGATAT+CGG - chr2.2:17648089-17648108 MS.gene060581:intron 30.0%
TATTCCAATTTACAACACAG+TGG + chr2.2:17647844-17647863 None:intergenic 30.0%
TATTGAAGAAAAAGAGTAGG+AGG - chr2.2:17648253-17648272 MS.gene060581:intron 30.0%
TCTACGAGTTAATTTCATGT+AGG - chr2.2:17647738-17647757 MS.gene060581:intron 30.0%
!! CATGTAAGTAAGTACTGTTT+TGG - chr2.2:17647903-17647922 MS.gene060581:intron 30.0%
!! GTTTTGGCAAAAGGATTATT+TGG - chr2.2:17647919-17647938 MS.gene060581:intron 30.0%
!! TTCTCCAGTTTTGTAAAGTA+GGG - chr2.2:17648896-17648915 MS.gene060581:CDS 30.0%
!!! CAAAAGGCTGAATTTTTTGT+AGG + chr2.2:17648578-17648597 None:intergenic 30.0%
AAAATCACGAGTTAACTCGT+AGG - chr2.2:17647397-17647416 MS.gene060581:intron 35.0%
AAGATAGAGGTAAAATGTGG+AGG + chr2.2:17647873-17647892 None:intergenic 35.0%
ACTCGTAGGATCATATGTTT+AGG - chr2.2:17647411-17647430 MS.gene060581:intron 35.0%
ACTTCCTTGTGAAAAGTCAT+CGG + chr2.2:17648152-17648171 None:intergenic 35.0%
ATAATATCCGCAAGAAGTTC+TGG + chr2.2:17646826-17646845 None:intergenic 35.0%
CAAGGAAGTAATGCTTATGT+TGG - chr2.2:17648163-17648182 MS.gene060581:intron 35.0%
CTATCTATGAAGCATGGATA+CGG - chr2.2:17647126-17647145 MS.gene060581:CDS 35.0%
GTACTTACTTACATGGTGTA+AGG + chr2.2:17647898-17647917 None:intergenic 35.0%
TTAATATTCACCCCTTTGTC+CGG + chr2.2:17648294-17648313 None:intergenic 35.0%
TTTCCCTTCTTGTCTTAAAC+AGG - chr2.2:17646978-17646997 MS.gene060581:CDS 35.0%
! CAACAATCTTTTGCTTTGTC+TGG - chr2.2:17648373-17648392 MS.gene060581:intron 35.0%
! GATATCTTCTAGCTGCTAAT+AGG + chr2.2:17648090-17648109 None:intergenic 35.0%
! GTGATTTTTCCGTTAAGAGA+AGG + chr2.2:17646958-17646977 None:intergenic 35.0%
! TGGTTTTTCTTTCCTGAATG+AGG + chr2.2:17648231-17648250 None:intergenic 35.0%
!! ATTAAACGAGTTGATGTCAC+CGG - chr2.2:17646844-17646863 MS.gene060581:CDS 35.0%
!! CTTCTCCAGTTTTGTAAAGT+AGG - chr2.2:17648895-17648914 MS.gene060581:CDS 35.0%
!! TCTCCAGTTTTGTAAAGTAG+GGG - chr2.2:17648897-17648916 MS.gene060581:CDS 35.0%
!!! GTAAGTACTGTTTTGGCAAA+AGG - chr2.2:17647910-17647929 MS.gene060581:intron 35.0%
!!! TTGGACGTTTTGGTTTCAAA+AGG + chr2.2:17648594-17648613 None:intergenic 35.0%
!!! TTTTGAAACCAAAACGTCCA+AGG - chr2.2:17648593-17648612 MS.gene060581:intron 35.0%
ATCAGTGAAGAACTTTCAGC+TGG - chr2.2:17648819-17648838 MS.gene060581:CDS 40.0%
ATGCTTATGTTGGCAAGCTA+AGG - chr2.2:17648173-17648192 MS.gene060581:intron 40.0%
CAATTTACAACACAGTGGTG+GGG + chr2.2:17647839-17647858 None:intergenic 40.0%
CAGATACTTCAACAATGCTC+AGG - chr2.2:17646729-17646748 MS.gene060581:CDS 40.0%
CCAATTTACAACACAGTGGT+GGG + chr2.2:17647840-17647859 None:intergenic 40.0%
CTGTCATATCCTTTCTCTCA+TGG + chr2.2:17648795-17648814 None:intergenic 40.0%
CTTCACAATTTATGACGCCT+CGG - chr2.2:17648453-17648472 MS.gene060581:intron 40.0%
CTTGAACTTCCATGAGAGAA+AGG - chr2.2:17648783-17648802 MS.gene060581:CDS 40.0%
GCCAAAGCTAGTTAAGCAAA+TGG + chr2.2:17648977-17648996 None:intergenic 40.0%
GCTATCTGAACTTTCGCATA+TGG + chr2.2:17648653-17648672 None:intergenic 40.0%
GGCAAGATAGAGGTAAAATG+TGG + chr2.2:17647876-17647895 None:intergenic 40.0%
TAAGGGCTGTCTATAGTACA+TGG + chr2.2:17648935-17648954 None:intergenic 40.0%
TAATATTCACCCCTTTGTCC+GGG + chr2.2:17648293-17648312 None:intergenic 40.0%
TAGTGGCTATCTATGAAGCA+TGG - chr2.2:17647120-17647139 MS.gene060581:CDS 40.0%
TATGAAGCACAAACACACCT+CGG + chr2.2:17647288-17647307 None:intergenic 40.0%
TCCAATTTACAACACAGTGG+TGG + chr2.2:17647841-17647860 None:intergenic 40.0%
TGAACTTTCGCATATGGATG+AGG + chr2.2:17648647-17648666 None:intergenic 40.0%
TGAAGAATAAAGCTCCGATG+TGG - chr2.2:17648737-17648756 MS.gene060581:CDS 40.0%
TGAGTTTAAACCCGGACAAA+GGG - chr2.2:17648280-17648299 MS.gene060581:intron 40.0%
TTAAGCAAATGGCAAACGCA+TGG + chr2.2:17648966-17648985 None:intergenic 40.0%
TTCCCTGTATCTGTTAATGC+TGG + chr2.2:17648064-17648083 None:intergenic 40.0%
TTGAGTTTAAACCCGGACAA+AGG - chr2.2:17648279-17648298 MS.gene060581:intron 40.0%
TTTGTCCGGGTTTAAACTCA+AGG + chr2.2:17648280-17648299 None:intergenic 40.0%
! AGGACCTGTTTAAGACAAGA+AGG + chr2.2:17646985-17647004 None:intergenic 40.0%
! GCCATTTGCTTAACTAGCTT+TGG - chr2.2:17648973-17648992 MS.gene060581:CDS 40.0%
! GGACCTGTTTAAGACAAGAA+GGG + chr2.2:17646984-17647003 None:intergenic 40.0%
! TGTTCTGTCACTTGTTGGTT+TGG + chr2.2:17648485-17648504 None:intergenic 40.0%
!! CTCCAGTTTTGTAAAGTAGG+GGG - chr2.2:17648898-17648917 MS.gene060581:CDS 40.0%
!! TTAAACGAGTTGATGTCACC+GGG - chr2.2:17646845-17646864 MS.gene060581:CDS 40.0%
!!! ACTCTCGATTTTGACTACCT+TGG - chr2.2:17647779-17647798 MS.gene060581:intron 40.0%
AAACGCATGGAAGGCAGATA+AGG + chr2.2:17648953-17648972 None:intergenic 45.0%
AACGCATGGAAGGCAGATAA+GGG + chr2.2:17648952-17648971 None:intergenic 45.0%
AGAGAGCAAATCATTCGCCT+TGG + chr2.2:17648613-17648632 None:intergenic 45.0%
ATATCACACGTGAGGTCGTA+GGG - chr2.2:17646929-17646948 MS.gene060581:CDS 45.0%
ATGAAACCAAGTTACTCGGC+TGG - chr2.2:17647485-17647504 MS.gene060581:intron 45.0%
ATGAAGCACAAACACACCTC+GGG + chr2.2:17647287-17647306 None:intergenic 45.0%
ATGAAGCATGGATACGGACA+CGG - chr2.2:17647132-17647151 MS.gene060581:CDS 45.0%
ATGGTGTAAGGGCAAGATAG+AGG + chr2.2:17647886-17647905 None:intergenic 45.0%
CAACAAGTGACAGAACAAGC+CGG - chr2.2:17648488-17648507 MS.gene060581:intron 45.0%
CACCCCCTACTTTACAAAAC+TGG + chr2.2:17648903-17648922 None:intergenic 45.0%
CATCGGAGGACAAAACTGAT+TGG - chr2.2:17646788-17646807 MS.gene060581:CDS 45.0%
CATGCTTCCAGAACTTCTTG+CGG - chr2.2:17646816-17646835 MS.gene060581:CDS 45.0%
CCCACCACTGTGTTGTAAAT+TGG - chr2.2:17647837-17647856 MS.gene060581:CDS 45.0%
CGCCAGCATTAACAGATACA+GGG - chr2.2:17648059-17648078 MS.gene060581:intron 45.0%
GAGGACCTTGAGTTTAAACC+CGG - chr2.2:17648272-17648291 MS.gene060581:intron 45.0%
GAGGTGTGTTTGTGCTTCAT+AGG - chr2.2:17647287-17647306 MS.gene060581:intron 45.0%
GAGTTTAAACCCGGACAAAG+GGG - chr2.2:17648281-17648300 MS.gene060581:intron 45.0%
GCTGGAAGTCATCAAAACCA+AGG + chr2.2:17647799-17647818 None:intergenic 45.0%
GTAGGGTCACCTTCTCTTAA+CGG - chr2.2:17646946-17646965 MS.gene060581:CDS 45.0%
GTGTGTTTGTGCTTCATAGG+TGG - chr2.2:17647290-17647309 MS.gene060581:intron 45.0%
TACAAGTGTCAGTGTCGTGT+CGG - chr2.2:17647221-17647240 MS.gene060581:CDS 45.0%
TCCAATTGCTCGTTCCACAT+CGG + chr2.2:17648754-17648773 None:intergenic 45.0%
TCGCCAGCATTAACAGATAC+AGG - chr2.2:17648058-17648077 MS.gene060581:intron 45.0%
TCTGTTTGCTTGCAAAACGC+CGG + chr2.2:17648510-17648529 None:intergenic 45.0%
! ATCATTCGCCTTGGACGTTT+TGG + chr2.2:17648604-17648623 None:intergenic 45.0%
! GATGCCGATGACTTTTCACA+AGG - chr2.2:17648145-17648164 MS.gene060581:intron 45.0%
!! AACTAGCTTTGGCACCAAAC+TGG - chr2.2:17648984-17649003 MS.gene060581:CDS 45.0%
!! GTTTTGATGACTTCCAGCCA+GGG - chr2.2:17647801-17647820 MS.gene060581:intron 45.0%
!! TAAACGAGTTGATGTCACCG+GGG - chr2.2:17646846-17646865 MS.gene060581:CDS 45.0%
!! CATATATATTATTTAATATT+AGG - chr2.2:17647701-17647720 MS.gene060581:intron 5.0%
ACAATTTATGACGCCTCGGC+TGG - chr2.2:17648457-17648476 MS.gene060581:intron 50.0%
ACGTTACCAGCAGGAACTTG+AGG + chr2.2:17648538-17648557 None:intergenic 50.0%
ACTGTGGATCAAAGCCAACC+AGG - chr2.2:17648847-17648866 MS.gene060581:CDS 50.0%
ATAAGCCCTCAAGTTCCTGC+TGG - chr2.2:17648529-17648548 MS.gene060581:intron 50.0%
ATACGGACACGGATACGACA+CGG - chr2.2:17647143-17647162 MS.gene060581:CDS 50.0%
CGTTACCAGCAGGAACTTGA+GGG + chr2.2:17648537-17648556 None:intergenic 50.0%
CTTGTGCCTGCGTTTGCAAA+CGG - chr2.2:17646899-17646918 MS.gene060581:CDS 50.0%
CTTTCAGCTGGTTGCTACTG+TGG - chr2.2:17648831-17648850 MS.gene060581:CDS 50.0%
CTTTCGCATATGGATGAGGC+AGG + chr2.2:17648643-17648662 None:intergenic 50.0%
GATATCACACGTGAGGTCGT+AGG - chr2.2:17646928-17646947 MS.gene060581:CDS 50.0%
GCAAATGGCAAACGCATGGA+AGG + chr2.2:17648962-17648981 None:intergenic 50.0%
GTGGAGAGATATCACACGTG+AGG - chr2.2:17646921-17646940 MS.gene060581:CDS 50.0%
TCCGATGTGGAACGAGCAAT+TGG - chr2.2:17648750-17648769 MS.gene060581:CDS 50.0%
TGGATCAAAGCCAACCAGGA+GGG - chr2.2:17648851-17648870 MS.gene060581:CDS 50.0%
TTGATGTCACCGGGGAACAA+TGG - chr2.2:17646854-17646873 MS.gene060581:CDS 50.0%
TTTGTCCTCCGATGCACTAC+CGG + chr2.2:17646782-17646801 None:intergenic 50.0%
! CGGCTTGTTCTGTCACTTGT+TGG + chr2.2:17648490-17648509 None:intergenic 50.0%
! TTACAACACAGTGGTGGGGA+AGG + chr2.2:17647835-17647854 None:intergenic 50.0%
!! CGCAAGAAGTTCTGGAAGCA+TGG + chr2.2:17646818-17646837 None:intergenic 50.0%
!! GGTTTTGATGACTTCCAGCC+AGG - chr2.2:17647800-17647819 MS.gene060581:intron 50.0%
ACGAGTCCAGCCGAGTAACT+TGG + chr2.2:17647494-17647513 None:intergenic 55.0%
GCGACGACATTTCGACGATG+CGG + chr2.2:17646880-17646899 None:intergenic 55.0%
GCTCAGGCTCTCAATGCTAC+CGG - chr2.2:17646745-17646764 MS.gene060581:CDS 55.0%
GGAACGAGCAATTGGAGTGC+TGG - chr2.2:17648758-17648777 MS.gene060581:CDS 55.0%
GTGGATCAAAGCCAACCAGG+AGG - chr2.2:17648850-17648869 MS.gene060581:CDS 55.0%
TCTCCACCGTTTGCAAACGC+AGG + chr2.2:17646908-17646927 None:intergenic 55.0%
TCTTCATCTCCTCTCCCTCC+TGG + chr2.2:17648868-17648887 None:intergenic 55.0%
TGACACCACCGGTAGTGCAT+CGG - chr2.2:17646771-17646790 MS.gene060581:CDS 55.0%
! AGTTCCTGCTGGTAACGTCG+AGG - chr2.2:17648540-17648559 MS.gene060581:intron 55.0%
! TGTCAGTGTCGTGTCGGTGT+CGG - chr2.2:17647227-17647246 MS.gene060581:CDS 55.0%
ACGATGCGGCCATTGTTCCC+CGG + chr2.2:17646866-17646885 None:intergenic 60.0%
CAAAGCCAACCAGGAGGGAG+AGG - chr2.2:17648856-17648875 MS.gene060581:CDS 60.0%
CATCTCCTCTCCCTCCTGGT+TGG + chr2.2:17648864-17648883 None:intergenic 60.0%
GGTTAGACGTGTCGCGGTGT+CGG + chr2.2:17647266-17647285 None:intergenic 60.0%
GTGCCTGCGTTTGCAAACGG+TGG - chr2.2:17646902-17646921 MS.gene060581:CDS 60.0%
! ACCTCGGGTTAGACGTGTCG+CGG + chr2.2:17647272-17647291 None:intergenic 60.0%
! CTGCTGGTAACGTCGAGGCA+GGG - chr2.2:17648545-17648564 MS.gene060581:intron 60.0%
!! ACTACCGGTGGTGTCATCGC+CGG + chr2.2:17646767-17646786 None:intergenic 60.0%
!! TGTTGGTTTGGCACCAGCCG+AGG + chr2.2:17648473-17648492 None:intergenic 60.0%
ACCGCGACACGTCTAACCCG+AGG - chr2.2:17647268-17647287 MS.gene060581:intron 65.0%
CACCACCGGTAGTGCATCGG+AGG - chr2.2:17646774-17646793 MS.gene060581:CDS 65.0%
CCTGCCTCGACGTTACCAGC+AGG + chr2.2:17648547-17648566 None:intergenic 65.0%
GCTACCGGCGATGACACCAC+CGG - chr2.2:17646760-17646779 MS.gene060581:CDS 65.0%
GTCCTCCGATGCACTACCGG+TGG + chr2.2:17646779-17646798 None:intergenic 65.0%
! CCTGCTGGTAACGTCGAGGC+AGG - chr2.2:17648544-17648563 MS.gene060581:intron 65.0%
! CAGTGGTGGGGAAGGTCGCG+TGG + chr2.2:17647827-17647846 None:intergenic 70.0%
GAAGGTCGCGTGGCCCTGGC+TGG + chr2.2:17647817-17647836 None:intergenic 75.0%
TGGGGAAGGTCGCGTGGCCC+TGG + chr2.2:17647821-17647840 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 17646700 17649017 17646700 ID=MS.gene060581
chr2.2 mRNA 17646700 17649017 17646700 ID=MS.gene060581.t1;Parent=MS.gene060581
chr2.2 exon 17648718 17649017 17648718 ID=MS.gene060581.t1.exon1;Parent=MS.gene060581.t1
chr2.2 CDS 17648718 17649017 17648718 ID=cds.MS.gene060581.t1;Parent=MS.gene060581.t1
chr2.2 exon 17647812 17647899 17647812 ID=MS.gene060581.t1.exon2;Parent=MS.gene060581.t1
chr2.2 CDS 17647812 17647899 17647812 ID=cds.MS.gene060581.t1;Parent=MS.gene060581.t1
chr2.2 exon 17647523 17647652 17647523 ID=MS.gene060581.t1.exon3;Parent=MS.gene060581.t1
chr2.2 CDS 17647523 17647652 17647523 ID=cds.MS.gene060581.t1;Parent=MS.gene060581.t1
chr2.2 exon 17646700 17647285 17646700 ID=MS.gene060581.t1.exon4;Parent=MS.gene060581.t1
chr2.2 CDS 17646700 17647285 17646700 ID=cds.MS.gene060581.t1;Parent=MS.gene060581.t1
Gene Sequence

>MS.gene060581

ATGAAGCTCTTTCGATCTCTTAAATTCTTCAGATACTTCAACAATGCTCAGGCTCTCAATGCTACCGGCGATGACACCACCGGTAGTGCATCGGAGGACAAAACTGATTGGTCTACCATGCTTCCAGAACTTCTTGCGGATATTATTAAACGAGTTGATGTCACCGGGGAACAATGGCCGCATCGTCGAAATGTCGTCGCTTGTGCCTGCGTTTGCAAACGGTGGAGAGATATCACACGTGAGGTCGTAGGGTCACCTTCTCTTAACGGAAAAATCACTTTCCCTTCTTGTCTTAAACAGGTCCTGCAACAAAATGAATTCATTTTATTTTTCTCTCTTGAATAATTTATGGATCATGAAAAATTTGGATGTAGTAATACATTATTTTTCTTCATAATGAAGACTAACATTTTGTTCAATTAGTGGCTATCTATGAAGCATGGATACGGACACGGATACGACACGGACACTGACACACCGACACAACTAATAATTTGAAAATTCACATAATTCAGTGTAATTACAAGTGTCAGTGTCGTGTCGGTGTCGGACACGACACGTGTCCGACACCGCGACACGTCTAACCCGAGGTGTGTTTGTGCTTCATAGGTGGCTATTAAGTGTCTGATAATGAGACATCAATTGAATTAAGAGAGTTGGAGTTTGACAATCTTGTTATGTTTTGATAAAGGTTGTTAAAATCACGAGTTAACTCGTAGGATCATATGTTTAGGTATCAATTTTGAGTTGACTCGACTATAAACTACAATTAAGTTAACTCGAAAATGAAACCAAGTTACTCGGCTGGACTCGTAGTGAATTTGTTAACTCTTAAACTTTCAGAAATTTTTTGAAGAACATTTTTATCTCTTCTTTTTTCATCTCAATTTTCAAACTCAATTCCTTTTCTTCTTTAATAGTTTGATTTTGTTCATATCAATTTTTTCTTTTGAATTGAGTTAAAATTTTGAATTATTGATGCATTTATTAAGTATATAGGTCATATATATTATTTAATATTAGGCAAACTCGTACAAGTCTACGAGTTAATTTCATGTAGGCAAATTAAGTTTACTTAAACTCTCGATTTTGACTACCTTGGTTTTGATGACTTCCAGCCAGGGCCACGCGACCTTCCCCACCACTGTGTTGTAAATTGGAATAAGAAGACCTCCACATTTTACCTCTATCTTGCCCTTACACCATGTAAGTAAGTACTGTTTTGGCAAAAGGATTATTTGGTTATAACTATAATAATGATTAAAATTTCAAATGGAGATTGGACAATGCTTATTTTTCAAAATATTTCAACAAATATAACTTGATGATGTTGCACAACTTATGATTTCTTTTTTCAATCGCCAGCATTAACAGATACAGGGAAATTCCTATTAGCAGCTAGAAGATATCGGTGTGTTACGCATACTGAGTATGTTATATCGCTCGATGCCGATGACTTTTCACAAGGAAGTAATGCTTATGTTGGCAAGCTAAGGTAAATATTTCTTGTAACAGAAAATAAAATGATCCTCATTCAGGAAAGAAAAACCATTTATTGAAGAAAAAGAGTAGGAGGACCTTGAGTTTAAACCCGGACAAAGGGGTGAATATTAATCTAACAAACTAACTAATAACATCTGGCATTAAAAAAAACCATTTATTGAATTTATTCTCAACAATCTTTTGCTTTGTCTGGAGTACTGATCATTTAGTTGCTGAAATGTCTTCACAGTTCAGATTTTCTTGAAGCAAGCTTCACAATTTATGACGCCTCGGCTGGTGCCAAACCAACAAGTGACAGAACAAGCCGGCGTTTTGCAAGCAAACAGATAAGCCCTCAAGTTCCTGCTGGTAACGTCGAGGCAGGGAACATCTCCTACAAAAAATTCAGCCTTTTGAAACCAAAACGTCCAAGGCGAATGATTTGCTCTCTGAAGTCTCCTGCCTCATCCATATGCGAAAGTTCAGATAGCAAGTCTTTCGACAATCATAAGATGCACTGTAAAGAACAAGCTACTTCTTCTGCCTACACAATCTTGAAGAATAAAGCTCCGATGTGGAACGAGCAATTGGAGTGCTGGTTCTTGAACTTCCATGAGAGAAAGGATATGACAGTAGCATCAGTGAAGAACTTTCAGCTGGTTGCTACTGTGGATCAAAGCCAACCAGGAGGGAGAGGAGATGAAGAAACAGTTCTTCTCCAGTTTTGTAAAGTAGGGGGTGATACTTTCACCATGTACTATAGACAGCCCTTATCTGCCTTCCATGCGTTTGCCATTTGCTTAACTAGCTTTGGCACCAAACTGGCTTGTCAGTAA
Protein sequence

>MS.gene060581.t1

MKLFRSLKFFRYFNNAQALNATGDDTTGSASEDKTDWSTMLPELLADIIKRVDVTGEQWPHRRNVVACACVCKRWRDITREVVGSPSLNGKITFPSCLKQPGPRDLPHHCVVNWNKKTSTFYLYLALTPSLTDTGKFLLAARRYRCVTHTEYVISLDADDFSQGSNAYVGKLSSDFLEASFTIYDASAGAKPTSDRTSRRFASKQISPQVPAGNVEAGNISYKKFSLLKPKRPRRMICSLKSPASSICESSDSKSFDNHKMHCKEQATSSAYTILKNKAPMWNEQLECWFLNFHERKDMTVASVKNFQLVATVDQSQPGGRGDEETVLLQFCKVGGDTFTMYYRQPLSAFHAFAICLTSFGTKLACQ