AlfalfaGEDB | MS.gene060885

M. sativa cultivar XinJiangDaYe / MS.gene060885

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060885.t1	XP_003588895.2	75.1	201	47	2	1	198	1	201	3.30E-68	268.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060885.t1	G7I8B4	75.1	201	47	2	1	198	1	201	2.4e-68	268.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060885	MS.gene060887	0.96721	5.78E-127	-1.69E-46
MS.gene060885	MS.gene060888	0.801201	1.01E-48	-1.69E-46
MS.gene060885	MS.gene33141	0.807336	5.31E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060885.t1	MTR_1g014420	91.542	201	14	2	1	198	1	201	2.11e-103	307
MS.gene060885.t1	MTR_3g106140	50.251	199	83	5	1	199	1	183	5.24e-48	163
MS.gene060885.t1	MTR_7g095010	68.966	58	18	0	106	163	1	58	1.00e-18	77.0
MS.gene060885.t1	MTR_4g126290	65.625	64	22	0	106	169	1	64	8.59e-12	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060885.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060885.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060885.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060885.t1	AT2G25130	50.000	206	76	8	1	199	1	186	1.42e-41	146

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGGAGAATGTTGGTGTTT+TGG	0.267693	1.2:-8268565	None:intergenic
AATGCTCGCTAAGGAGAATT+TGG	0.287918	1.2:+8268947	MS.gene060885:CDS
TGAGGTTTCCGACGTTGTTC+CGG	0.317043	1.2:-8268515	None:intergenic
CTCTACGCATTGCTCAATCT+TGG	0.375202	1.2:+8269059	MS.gene060885:CDS
TCTCCCACCACTTCCGTCTC+TGG	0.384503	1.2:+8268580	MS.gene060885:CDS
AGTTTCTCCGATTTCACATT+CGG	0.396316	1.2:-8268777	None:intergenic
TGGTGGAACGGTGGTGGTGG+TGG	0.401730	1.2:-8268545	None:intergenic
TTCAAAACCGAATGTGAAAT+CGG	0.408736	1.2:+8268770	MS.gene060885:CDS
TCTTCTGAACATGGCGGAGA+TGG	0.446454	1.2:+8268806	MS.gene060885:CDS
GCGGTGATTGTAACGGGAGG+TGG	0.458786	1.2:-8268664	None:intergenic
TTCAGTGTCATATTCGTTGA+AGG	0.469707	1.2:-8268697	None:intergenic
ACGGCGGCGGTGATTGTAAC+GGG	0.473080	1.2:-8268670	None:intergenic
TTTGCATGAGGAAGATGATT+CGG	0.481809	1.2:+8268893	MS.gene060885:CDS
AAGGAGAATTTGGAAGTAAG+AGG	0.491585	1.2:+8268957	MS.gene060885:CDS
GGTGGTGGTGGCCGAGAATG+AGG	0.498770	1.2:-8268533	None:intergenic
AGAAGAGGAAGCGAAGAAGA+AGG	0.499264	1.2:+8268833	MS.gene060885:CDS
GCATTGCTCAATCTTGGAAT+CGG	0.503671	1.2:+8269065	MS.gene060885:CDS
CTCCGCCATGTTCAGAAGAT+CGG	0.507404	1.2:-8268802	None:intergenic
AACGTCGGAAACCTCATTCT+CGG	0.508681	1.2:+8268522	MS.gene060885:CDS
AGTTCCAGAGACGGAAGTGG+TGG	0.509557	1.2:-8268584	None:intergenic
ATCTTCCACGCCGTCAGCTG+CGG	0.516615	1.2:+8268636	MS.gene060885:CDS
TTCCACGCCGTCAGCTGCGG+TGG	0.526745	1.2:+8268639	MS.gene060885:CDS
CGTTGAAGGTGCTACGGCGG+CGG	0.537750	1.2:-8268683	None:intergenic
CAGCTTCCTCCTTCGACAAA+CGG	0.537814	1.2:+8268733	MS.gene060885:CDS
ATTCTGTTGCCGTTTGTCGA+AGG	0.541771	1.2:-8268742	None:intergenic
GAGAGTTCCAGAGACGGAAG+TGG	0.541905	1.2:-8268587	None:intergenic
CGGAAGTGGTGGGAGAATGT+TGG	0.542844	1.2:-8268573	None:intergenic
GAGACGACGGAGGGAGGCGA+TGG	0.543987	1.2:-8268613	None:intergenic
GAATCGAGCATTGCGACTAA+CGG	0.551485	1.2:-8269008	None:intergenic
TACGGCGGCGGTGATTGTAA+CGG	0.552541	1.2:-8268671	None:intergenic
AGAGGAACACTTTCAATGCT+CGG	0.553240	1.2:+8268975	MS.gene060885:CDS
GGTGGCGCCACCGCAGCTGA+CGG	0.558285	1.2:-8268646	None:intergenic
CACCGTGAGAATGCTCGCTA+AGG	0.563128	1.2:+8268938	MS.gene060885:CDS
CTCCTTAGCGAGCATTCTCA+CGG	0.567241	1.2:-8268940	None:intergenic
CGCCACCGCAGCTGACGGCG+TGG	0.567727	1.2:-8268641	None:intergenic
CTGTTGCCGTTTGTCGAAGG+AGG	0.569127	1.2:-8268739	None:intergenic
GAAACTTAAGAAGCTTGTGA+AGG	0.574556	1.2:+8268869	MS.gene060885:CDS
CATATTCGTTGAAGGTGCTA+CGG	0.583082	1.2:-8268689	None:intergenic
GAAGATGAGACGACGGAGGG+AGG	0.594413	1.2:-8268619	None:intergenic
TCGAGCATTGCGACTAACGG+TGG	0.599056	1.2:-8269005	None:intergenic
GGAGAATGGCAAACTATCAC+CGG	0.599573	1.2:+8268496	None:intergenic
AACTATCACCGGAACAACGT+CGG	0.600978	1.2:+8268507	MS.gene060885:CDS
GTTCCAGAGACGGAAGTGGT+GGG	0.614760	1.2:-8268583	None:intergenic
AGGAAGATGATTCGGTGAAG+CGG	0.615812	1.2:+8268901	MS.gene060885:CDS
GGCGGAGATGGAGACAGAAG+AGG	0.621626	1.2:+8268818	MS.gene060885:CDS
ACTCTCCGATCTTCTGAACA+TGG	0.624622	1.2:+8268797	MS.gene060885:CDS
TGGAGAGAGAGTTCCAGAGA+CGG	0.628731	1.2:-8268593	None:intergenic
GTGGAAGATGAGACGACGGA+GGG	0.638181	1.2:-8268622	None:intergenic
GCTTGTGAAGGATTTGCATG+AGG	0.641834	1.2:+8268881	MS.gene060885:CDS
ATTCGTTGAAGGTGCTACGG+CGG	0.652805	1.2:-8268686	None:intergenic
TTTGTCGAAGGAGGAAGCTG+AGG	0.654659	1.2:-8268730	None:intergenic
CGTGGAAGATGAGACGACGG+AGG	0.676052	1.2:-8268623	None:intergenic
CGGCGTGGAAGATGAGACGA+CGG	0.695582	1.2:-8268626	None:intergenic
GCGGCGGTGATTGTAACGGG+AGG	0.698798	1.2:-8268667	None:intergenic
CTTAGCGAGCATTCTCACGG+TGG	0.717190	1.2:-8268937	None:intergenic
CTCCGATCTTCTGAACATGG+CGG	0.718028	1.2:+8268800	MS.gene060885:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTCAAAACCGAATGTGAAAT+CGG	+	chr1.2:8268770-8268789	MS.gene060885:CDS	30.0%
	AAGGAGAATTTGGAAGTAAG+AGG	+	chr1.2:8268957-8268976	MS.gene060885:CDS	35.0%
	AGTTTCTCCGATTTCACATT+CGG	-	chr1.2:8268780-8268799	None:intergenic	35.0%
	GAAACTTAAGAAGCTTGTGA+AGG	+	chr1.2:8268869-8268888	MS.gene060885:CDS	35.0%
	TTCAGTGTCATATTCGTTGA+AGG	-	chr1.2:8268700-8268719	None:intergenic	35.0%
	TTTGCATGAGGAAGATGATT+CGG	+	chr1.2:8268893-8268912	MS.gene060885:CDS	35.0%
	AATGCTCGCTAAGGAGAATT+TGG	+	chr1.2:8268947-8268966	MS.gene060885:CDS	40.0%
	AGAGGAACACTTTCAATGCT+CGG	+	chr1.2:8268975-8268994	MS.gene060885:CDS	40.0%
	GCATTGCTCAATCTTGGAAT+CGG	+	chr1.2:8269065-8269084	MS.gene060885:CDS	40.0%
!!	CATATTCGTTGAAGGTGCTA+CGG	-	chr1.2:8268692-8268711	None:intergenic	40.0%
!!!	ATGTTGGTGTTTTGGTGGAA+CGG	-	chr1.2:8268560-8268579	None:intergenic	40.0%
	AACGTCGGAAACCTCATTCT+CGG	+	chr1.2:8268522-8268541	MS.gene060885:CDS	45.0%
	AACTATCACCGGAACAACGT+CGG	+	chr1.2:8268507-8268526	MS.gene060885:CDS	45.0%
	ACTCTCCGATCTTCTGAACA+TGG	+	chr1.2:8268797-8268816	MS.gene060885:CDS	45.0%
	AGAAGAGGAAGCGAAGAAGA+AGG	+	chr1.2:8268833-8268852	MS.gene060885:CDS	45.0%
	CTCTACGCATTGCTCAATCT+TGG	+	chr1.2:8269059-8269078	MS.gene060885:CDS	45.0%
	GCTTGTGAAGGATTTGCATG+AGG	+	chr1.2:8268881-8268900	MS.gene060885:CDS	45.0%
!	ATTCTGTTGCCGTTTGTCGA+AGG	-	chr1.2:8268745-8268764	None:intergenic	45.0%
!	GAATCGAGCATTGCGACTAA+CGG	-	chr1.2:8269011-8269030	None:intergenic	45.0%
!!	AGGAAGATGATTCGGTGAAG+CGG	+	chr1.2:8268901-8268920	MS.gene060885:CDS	45.0%
!!	GGAGAATGTTGGTGTTTTGG+TGG	-	chr1.2:8268565-8268584	None:intergenic	45.0%
!!	GTGGGAGAATGTTGGTGTTT+TGG	-	chr1.2:8268568-8268587	None:intergenic	45.0%
	CAGCTTCCTCCTTCGACAAA+CGG	+	chr1.2:8268733-8268752	MS.gene060885:CDS	50.0%
	CTCCGATCTTCTGAACATGG+CGG	+	chr1.2:8268800-8268819	MS.gene060885:CDS	50.0%
	CTCCGCCATGTTCAGAAGAT+CGG	-	chr1.2:8268805-8268824	None:intergenic	50.0%
	CTCCTTAGCGAGCATTCTCA+CGG	-	chr1.2:8268943-8268962	None:intergenic	50.0%
	TGAGGTTTCCGACGTTGTTC+CGG	-	chr1.2:8268518-8268537	None:intergenic	50.0%
	TGGAGAGAGAGTTCCAGAGA+CGG	-	chr1.2:8268596-8268615	None:intergenic	50.0%
	TTTGTCGAAGGAGGAAGCTG+AGG	-	chr1.2:8268733-8268752	None:intergenic	50.0%
!	TCTTCTGAACATGGCGGAGA+TGG	+	chr1.2:8268806-8268825	MS.gene060885:CDS	50.0%
!!	ATTCGTTGAAGGTGCTACGG+CGG	-	chr1.2:8268689-8268708	None:intergenic	50.0%
!!!	TTGGTGTTTTGGTGGAACGG+TGG	-	chr1.2:8268557-8268576	None:intergenic	50.0%
	AGTTCCAGAGACGGAAGTGG+TGG	-	chr1.2:8268587-8268606	None:intergenic	55.0%
	CACCGTGAGAATGCTCGCTA+AGG	+	chr1.2:8268938-8268957	MS.gene060885:CDS	55.0%
	CTTAGCGAGCATTCTCACGG+TGG	-	chr1.2:8268940-8268959	None:intergenic	55.0%
	GAGAGTTCCAGAGACGGAAG+TGG	-	chr1.2:8268590-8268609	None:intergenic	55.0%
	GTGGAAGATGAGACGACGGA+GGG	-	chr1.2:8268625-8268644	None:intergenic	55.0%
	GTTCCAGAGACGGAAGTGGT+GGG	-	chr1.2:8268586-8268605	None:intergenic	55.0%
	TACGGCGGCGGTGATTGTAA+CGG	-	chr1.2:8268674-8268693	None:intergenic	55.0%
!	CGGAAGTGGTGGGAGAATGT+TGG	-	chr1.2:8268576-8268595	None:intergenic	55.0%
!	CTGTTGCCGTTTGTCGAAGG+AGG	-	chr1.2:8268742-8268761	None:intergenic	55.0%
!	TCGAGCATTGCGACTAACGG+TGG	-	chr1.2:8269008-8269027	None:intergenic	55.0%
!!	TTTTGGTGGAACGGTGGTGG+TGG	-	chr1.2:8268551-8268570	None:intergenic	55.0%
!!!	GTGTTTTGGTGGAACGGTGG+TGG	-	chr1.2:8268554-8268573	None:intergenic	55.0%
	ACGGCGGCGGTGATTGTAAC+GGG	-	chr1.2:8268673-8268692	None:intergenic	60.0%
	ATCTTCCACGCCGTCAGCTG+CGG	+	chr1.2:8268636-8268655	MS.gene060885:CDS	60.0%
	CGGCGTGGAAGATGAGACGA+CGG	-	chr1.2:8268629-8268648	None:intergenic	60.0%
	CGTGGAAGATGAGACGACGG+AGG	-	chr1.2:8268626-8268645	None:intergenic	60.0%
	GAAGATGAGACGACGGAGGG+AGG	-	chr1.2:8268622-8268641	None:intergenic	60.0%
	GGCGGAGATGGAGACAGAAG+AGG	+	chr1.2:8268818-8268837	MS.gene060885:CDS	60.0%
	TCTCCCACCACTTCCGTCTC+TGG	+	chr1.2:8268580-8268599	MS.gene060885:CDS	60.0%
!	GCGGTGATTGTAACGGGAGG+TGG	-	chr1.2:8268667-8268686	None:intergenic	60.0%
!	GCGGCGGTGATTGTAACGGG+AGG	-	chr1.2:8268670-8268689	None:intergenic	65.0%
!!	CGTTGAAGGTGCTACGGCGG+CGG	-	chr1.2:8268686-8268705	None:intergenic	65.0%
!!	GGTGGTGGTGGCCGAGAATG+AGG	-	chr1.2:8268536-8268555	None:intergenic	65.0%
!!	TGGTGGAACGGTGGTGGTGG+TGG	-	chr1.2:8268548-8268567	None:intergenic	65.0%
	GAGACGACGGAGGGAGGCGA+TGG	-	chr1.2:8268616-8268635	None:intergenic	70.0%
	TTCCACGCCGTCAGCTGCGG+TGG	+	chr1.2:8268639-8268658	MS.gene060885:CDS	70.0%
	GGTGGCGCCACCGCAGCTGA+CGG	-	chr1.2:8268649-8268668	None:intergenic	75.0%
!	CGCCACCGCAGCTGACGGCG+TGG	-	chr1.2:8268644-8268663	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	8268501	8269100	8268501	ID=MS.gene060885
chr1.2	mRNA	8268501	8269100	8268501	ID=MS.gene060885.t1;Parent=MS.gene060885
chr1.2	exon	8268501	8269100	8268501	ID=MS.gene060885.t1.exon1;Parent=MS.gene060885.t1
chr1.2	CDS	8268501	8269100	8268501	ID=cds.MS.gene060885.t1;Parent=MS.gene060885.t1

Gene Sequence

>MS.gene060885

ATGGCAAACTATCACCGGAACAACGTCGGAAACCTCATTCTCGGCCACCACCACCACCGTTCCACCAAAACACCAACATTCTCCCACCACTTCCGTCTCTGGAACTCTCTCTCCATCGCCTCCCTCCGTCGTCTCATCTTCCACGCCGTCAGCTGCGGTGGCGCCACCTCCCGTTACAATCACCGCCGCCGTAGCACCTTCAACGAATATGACACTGAAGACTTCTCTTCCTCAGCTTCCTCCTTCGACAAACGGCAACAGAATCAAACTTCAAAACCGAATGTGAAATCGGAGAAACTCTCCGATCTTCTGAACATGGCGGAGATGGAGACAGAAGAGGAAGCGAAGAAGAAGGAAGAAGCTCTAGAGAAACTTAAGAAGCTTGTGAAGGATTTGCATGAGGAAGATGATTCGGTGAAGCGGCGTGAAGCTGCGACCACCGTGAGAATGCTCGCTAAGGAGAATTTGGAAGTAAGAGGAACACTTTCAATGCTCGGAGCGATTCCACCGTTAGTCGCAATGCTCGATTCAAAAGACGTTGATTCTCAGATCGCTTCTCTCTACGCATTGCTCAATCTTGGAATCGGAAACGATGCGTGA

Protein sequence

>MS.gene060885.t1

MANYHRNNVGNLILGHHHHRSTKTPTFSHHFRLWNSLSIASLRRLIFHAVSCGGATSRYNHRRRSTFNEYDTEDFSSSASSFDKRQQNQTSKPNVKSEKLSDLLNMAEMETEEEAKKKEEALEKLKKLVKDLHEEDDSVKRREAATTVRMLAKENLEVRGTLSMLGAIPPLVAMLDSKDVDSQIASLYALLNLGIGNDA