AlfalfaGEDB | MS.gene060887

M. sativa cultivar XinJiangDaYe / MS.gene060887

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060887.t1	XP_003588895.2	75.1	201	47	2	1	198	1	201	3.30E-68	268.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060887.t1	G7I8B4	75.1	201	47	2	1	198	1	201	2.4e-68	268.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051087	MS.gene060887	0.801938	7.11E-49	-1.69E-46
MS.gene060885	MS.gene060887	0.96721	5.78E-127	-1.69E-46
MS.gene060887	MS.gene060888	0.816866	4.43E-52	-1.69E-46
MS.gene060887	MS.gene33141	0.809309	2.02E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060887.t1	MTR_1g014420	91.542	201	14	2	1	198	1	201	2.11e-103	307
MS.gene060887.t1	MTR_3g106140	50.251	199	83	5	1	199	1	183	5.24e-48	163
MS.gene060887.t1	MTR_7g095010	68.966	58	18	0	106	163	1	58	1.00e-18	77.0
MS.gene060887.t1	MTR_4g126290	65.625	64	22	0	106	169	1	64	8.59e-12	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060887.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060887.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060887.t1	AT4G31890	49.554	224	83	8	1	199	1	219	2.90e-51	172
MS.gene060887.t1	AT2G25130	50.000	206	76	8	1	199	1	186	1.42e-41	146

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGGAGAATGTTGGTGTTT+TGG	0.267693	1.2:-8253289	None:intergenic
AATGCTCGCTAAGGAGAATT+TGG	0.287918	1.2:+8253671	MS.gene060887:CDS
TGAGGTTTCCGACGTTGTTC+CGG	0.317043	1.2:-8253239	None:intergenic
CTCTACGCATTGCTCAATCT+TGG	0.375202	1.2:+8253783	MS.gene060887:CDS
TCTCCCACCACTTCCGTCTC+TGG	0.384503	1.2:+8253304	MS.gene060887:CDS
AGTTTCTCCGATTTCACATT+CGG	0.396316	1.2:-8253501	None:intergenic
TGGTGGAACGGTGGTGGTGG+TGG	0.401730	1.2:-8253269	None:intergenic
TTCAAAACCGAATGTGAAAT+CGG	0.408736	1.2:+8253494	MS.gene060887:CDS
TCTTCTGAACATGGCGGAGA+TGG	0.446454	1.2:+8253530	MS.gene060887:CDS
GCGGTGATTGTAACGGGAGG+TGG	0.458786	1.2:-8253388	None:intergenic
TTCAGTGTCATATTCGTTGA+AGG	0.469707	1.2:-8253421	None:intergenic
ACGGCGGCGGTGATTGTAAC+GGG	0.473080	1.2:-8253394	None:intergenic
TTTGCATGAGGAAGATGATT+CGG	0.481809	1.2:+8253617	MS.gene060887:CDS
AAGGAGAATTTGGAAGTAAG+AGG	0.491585	1.2:+8253681	MS.gene060887:CDS
GGTGGTGGTGGCCGAGAATG+AGG	0.498770	1.2:-8253257	None:intergenic
AGAAGAGGAAGCGAAGAAGA+AGG	0.499264	1.2:+8253557	MS.gene060887:CDS
GCATTGCTCAATCTTGGAAT+CGG	0.503671	1.2:+8253789	MS.gene060887:CDS
CTCCGCCATGTTCAGAAGAT+CGG	0.507404	1.2:-8253526	None:intergenic
AACGTCGGAAACCTCATTCT+CGG	0.508681	1.2:+8253246	MS.gene060887:CDS
AGTTCCAGAGACGGAAGTGG+TGG	0.509557	1.2:-8253308	None:intergenic
ATCTTCCACGCCGTCAGCTG+CGG	0.516615	1.2:+8253360	MS.gene060887:CDS
TTCCACGCCGTCAGCTGCGG+TGG	0.526745	1.2:+8253363	MS.gene060887:CDS
CGTTGAAGGTGCTACGGCGG+CGG	0.537750	1.2:-8253407	None:intergenic
CAGCTTCCTCCTTCGACAAA+CGG	0.537814	1.2:+8253457	MS.gene060887:CDS
ATTCTGTTGCCGTTTGTCGA+AGG	0.541771	1.2:-8253466	None:intergenic
GAGAGTTCCAGAGACGGAAG+TGG	0.541905	1.2:-8253311	None:intergenic
CGGAAGTGGTGGGAGAATGT+TGG	0.542844	1.2:-8253297	None:intergenic
GAGACGACGGAGGGAGGCGA+TGG	0.543987	1.2:-8253337	None:intergenic
GAATCGAGCATTGCGACTAA+CGG	0.551485	1.2:-8253732	None:intergenic
TACGGCGGCGGTGATTGTAA+CGG	0.552541	1.2:-8253395	None:intergenic
AGAGGAACACTTTCAATGCT+CGG	0.553240	1.2:+8253699	MS.gene060887:CDS
GGTGGCGCCACCGCAGCTGA+CGG	0.558285	1.2:-8253370	None:intergenic
CACCGTGAGAATGCTCGCTA+AGG	0.563128	1.2:+8253662	MS.gene060887:CDS
CTCCTTAGCGAGCATTCTCA+CGG	0.567241	1.2:-8253664	None:intergenic
CGCCACCGCAGCTGACGGCG+TGG	0.567727	1.2:-8253365	None:intergenic
CTGTTGCCGTTTGTCGAAGG+AGG	0.569127	1.2:-8253463	None:intergenic
GAAACTTAAGAAGCTTGTGA+AGG	0.574556	1.2:+8253593	MS.gene060887:CDS
CATATTCGTTGAAGGTGCTA+CGG	0.583082	1.2:-8253413	None:intergenic
GAAGATGAGACGACGGAGGG+AGG	0.594413	1.2:-8253343	None:intergenic
TCGAGCATTGCGACTAACGG+TGG	0.599056	1.2:-8253729	None:intergenic
GGAGAATGGCAAACTATCAC+CGG	0.599573	1.2:+8253220	None:intergenic
AACTATCACCGGAACAACGT+CGG	0.600978	1.2:+8253231	MS.gene060887:CDS
GTTCCAGAGACGGAAGTGGT+GGG	0.614760	1.2:-8253307	None:intergenic
AGGAAGATGATTCGGTGAAG+CGG	0.615812	1.2:+8253625	MS.gene060887:CDS
GGCGGAGATGGAGACAGAAG+AGG	0.621626	1.2:+8253542	MS.gene060887:CDS
ACTCTCCGATCTTCTGAACA+TGG	0.624622	1.2:+8253521	MS.gene060887:CDS
TGGAGAGAGAGTTCCAGAGA+CGG	0.628731	1.2:-8253317	None:intergenic
GTGGAAGATGAGACGACGGA+GGG	0.638181	1.2:-8253346	None:intergenic
GCTTGTGAAGGATTTGCATG+AGG	0.641834	1.2:+8253605	MS.gene060887:CDS
ATTCGTTGAAGGTGCTACGG+CGG	0.652805	1.2:-8253410	None:intergenic
TTTGTCGAAGGAGGAAGCTG+AGG	0.654659	1.2:-8253454	None:intergenic
CGTGGAAGATGAGACGACGG+AGG	0.676052	1.2:-8253347	None:intergenic
CGGCGTGGAAGATGAGACGA+CGG	0.695582	1.2:-8253350	None:intergenic
GCGGCGGTGATTGTAACGGG+AGG	0.698798	1.2:-8253391	None:intergenic
CTTAGCGAGCATTCTCACGG+TGG	0.717190	1.2:-8253661	None:intergenic
CTCCGATCTTCTGAACATGG+CGG	0.718028	1.2:+8253524	MS.gene060887:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTCAAAACCGAATGTGAAAT+CGG	+	chr1.2:8253494-8253513	MS.gene060887:CDS	30.0%
	AAGGAGAATTTGGAAGTAAG+AGG	+	chr1.2:8253681-8253700	MS.gene060887:CDS	35.0%
	AGTTTCTCCGATTTCACATT+CGG	-	chr1.2:8253504-8253523	None:intergenic	35.0%
	GAAACTTAAGAAGCTTGTGA+AGG	+	chr1.2:8253593-8253612	MS.gene060887:CDS	35.0%
	TTCAGTGTCATATTCGTTGA+AGG	-	chr1.2:8253424-8253443	None:intergenic	35.0%
	TTTGCATGAGGAAGATGATT+CGG	+	chr1.2:8253617-8253636	MS.gene060887:CDS	35.0%
	AATGCTCGCTAAGGAGAATT+TGG	+	chr1.2:8253671-8253690	MS.gene060887:CDS	40.0%
	AGAGGAACACTTTCAATGCT+CGG	+	chr1.2:8253699-8253718	MS.gene060887:CDS	40.0%
	GCATTGCTCAATCTTGGAAT+CGG	+	chr1.2:8253789-8253808	MS.gene060887:CDS	40.0%
!!	CATATTCGTTGAAGGTGCTA+CGG	-	chr1.2:8253416-8253435	None:intergenic	40.0%
!!!	ATGTTGGTGTTTTGGTGGAA+CGG	-	chr1.2:8253284-8253303	None:intergenic	40.0%
	AACGTCGGAAACCTCATTCT+CGG	+	chr1.2:8253246-8253265	MS.gene060887:CDS	45.0%
	AACTATCACCGGAACAACGT+CGG	+	chr1.2:8253231-8253250	MS.gene060887:CDS	45.0%
	ACTCTCCGATCTTCTGAACA+TGG	+	chr1.2:8253521-8253540	MS.gene060887:CDS	45.0%
	AGAAGAGGAAGCGAAGAAGA+AGG	+	chr1.2:8253557-8253576	MS.gene060887:CDS	45.0%
	CTCTACGCATTGCTCAATCT+TGG	+	chr1.2:8253783-8253802	MS.gene060887:CDS	45.0%
	GCTTGTGAAGGATTTGCATG+AGG	+	chr1.2:8253605-8253624	MS.gene060887:CDS	45.0%
!	ATTCTGTTGCCGTTTGTCGA+AGG	-	chr1.2:8253469-8253488	None:intergenic	45.0%
!	GAATCGAGCATTGCGACTAA+CGG	-	chr1.2:8253735-8253754	None:intergenic	45.0%
!!	AGGAAGATGATTCGGTGAAG+CGG	+	chr1.2:8253625-8253644	MS.gene060887:CDS	45.0%
!!	GGAGAATGTTGGTGTTTTGG+TGG	-	chr1.2:8253289-8253308	None:intergenic	45.0%
!!	GTGGGAGAATGTTGGTGTTT+TGG	-	chr1.2:8253292-8253311	None:intergenic	45.0%
	CAGCTTCCTCCTTCGACAAA+CGG	+	chr1.2:8253457-8253476	MS.gene060887:CDS	50.0%
	CTCCGATCTTCTGAACATGG+CGG	+	chr1.2:8253524-8253543	MS.gene060887:CDS	50.0%
	CTCCGCCATGTTCAGAAGAT+CGG	-	chr1.2:8253529-8253548	None:intergenic	50.0%
	CTCCTTAGCGAGCATTCTCA+CGG	-	chr1.2:8253667-8253686	None:intergenic	50.0%
	TGAGGTTTCCGACGTTGTTC+CGG	-	chr1.2:8253242-8253261	None:intergenic	50.0%
	TGGAGAGAGAGTTCCAGAGA+CGG	-	chr1.2:8253320-8253339	None:intergenic	50.0%
	TTTGTCGAAGGAGGAAGCTG+AGG	-	chr1.2:8253457-8253476	None:intergenic	50.0%
!	TCTTCTGAACATGGCGGAGA+TGG	+	chr1.2:8253530-8253549	MS.gene060887:CDS	50.0%
!!	ATTCGTTGAAGGTGCTACGG+CGG	-	chr1.2:8253413-8253432	None:intergenic	50.0%
!!!	TTGGTGTTTTGGTGGAACGG+TGG	-	chr1.2:8253281-8253300	None:intergenic	50.0%
	AGTTCCAGAGACGGAAGTGG+TGG	-	chr1.2:8253311-8253330	None:intergenic	55.0%
	CACCGTGAGAATGCTCGCTA+AGG	+	chr1.2:8253662-8253681	MS.gene060887:CDS	55.0%
	CTTAGCGAGCATTCTCACGG+TGG	-	chr1.2:8253664-8253683	None:intergenic	55.0%
	GAGAGTTCCAGAGACGGAAG+TGG	-	chr1.2:8253314-8253333	None:intergenic	55.0%
	GTGGAAGATGAGACGACGGA+GGG	-	chr1.2:8253349-8253368	None:intergenic	55.0%
	GTTCCAGAGACGGAAGTGGT+GGG	-	chr1.2:8253310-8253329	None:intergenic	55.0%
	TACGGCGGCGGTGATTGTAA+CGG	-	chr1.2:8253398-8253417	None:intergenic	55.0%
!	CGGAAGTGGTGGGAGAATGT+TGG	-	chr1.2:8253300-8253319	None:intergenic	55.0%
!	CTGTTGCCGTTTGTCGAAGG+AGG	-	chr1.2:8253466-8253485	None:intergenic	55.0%
!	TCGAGCATTGCGACTAACGG+TGG	-	chr1.2:8253732-8253751	None:intergenic	55.0%
!!	TTTTGGTGGAACGGTGGTGG+TGG	-	chr1.2:8253275-8253294	None:intergenic	55.0%
!!!	GTGTTTTGGTGGAACGGTGG+TGG	-	chr1.2:8253278-8253297	None:intergenic	55.0%
	ACGGCGGCGGTGATTGTAAC+GGG	-	chr1.2:8253397-8253416	None:intergenic	60.0%
	ATCTTCCACGCCGTCAGCTG+CGG	+	chr1.2:8253360-8253379	MS.gene060887:CDS	60.0%
	CGGCGTGGAAGATGAGACGA+CGG	-	chr1.2:8253353-8253372	None:intergenic	60.0%
	CGTGGAAGATGAGACGACGG+AGG	-	chr1.2:8253350-8253369	None:intergenic	60.0%
	GAAGATGAGACGACGGAGGG+AGG	-	chr1.2:8253346-8253365	None:intergenic	60.0%
	GGCGGAGATGGAGACAGAAG+AGG	+	chr1.2:8253542-8253561	MS.gene060887:CDS	60.0%
	TCTCCCACCACTTCCGTCTC+TGG	+	chr1.2:8253304-8253323	MS.gene060887:CDS	60.0%
!	GCGGTGATTGTAACGGGAGG+TGG	-	chr1.2:8253391-8253410	None:intergenic	60.0%
!	GCGGCGGTGATTGTAACGGG+AGG	-	chr1.2:8253394-8253413	None:intergenic	65.0%
!!	CGTTGAAGGTGCTACGGCGG+CGG	-	chr1.2:8253410-8253429	None:intergenic	65.0%
!!	GGTGGTGGTGGCCGAGAATG+AGG	-	chr1.2:8253260-8253279	None:intergenic	65.0%
!!	TGGTGGAACGGTGGTGGTGG+TGG	-	chr1.2:8253272-8253291	None:intergenic	65.0%
	GAGACGACGGAGGGAGGCGA+TGG	-	chr1.2:8253340-8253359	None:intergenic	70.0%
	TTCCACGCCGTCAGCTGCGG+TGG	+	chr1.2:8253363-8253382	MS.gene060887:CDS	70.0%
	GGTGGCGCCACCGCAGCTGA+CGG	-	chr1.2:8253373-8253392	None:intergenic	75.0%
!	CGCCACCGCAGCTGACGGCG+TGG	-	chr1.2:8253368-8253387	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	8253225	8253824	8253225	ID=MS.gene060887
chr1.2	mRNA	8253225	8253824	8253225	ID=MS.gene060887.t1;Parent=MS.gene060887
chr1.2	exon	8253225	8253824	8253225	ID=MS.gene060887.t1.exon1;Parent=MS.gene060887.t1
chr1.2	CDS	8253225	8253824	8253225	ID=cds.MS.gene060887.t1;Parent=MS.gene060887.t1

Gene Sequence

>MS.gene060887

ATGGCAAACTATCACCGGAACAACGTCGGAAACCTCATTCTCGGCCACCACCACCACCGTTCCACCAAAACACCAACATTCTCCCACCACTTCCGTCTCTGGAACTCTCTCTCCATCGCCTCCCTCCGTCGTCTCATCTTCCACGCCGTCAGCTGCGGTGGCGCCACCTCCCGTTACAATCACCGCCGCCGTAGCACCTTCAACGAATATGACACTGAAGACTTCTCTTCCTCAGCTTCCTCCTTCGACAAACGGCAACAGAATCAAACTTCAAAACCGAATGTGAAATCGGAGAAACTCTCCGATCTTCTGAACATGGCGGAGATGGAGACAGAAGAGGAAGCGAAGAAGAAGGAAGAAGCTCTAGAGAAACTTAAGAAGCTTGTGAAGGATTTGCATGAGGAAGATGATTCGGTGAAGCGGCGTGAAGCTGCGACCACCGTGAGAATGCTCGCTAAGGAGAATTTGGAAGTAAGAGGAACACTTTCAATGCTCGGAGCGATTCCACCGTTAGTCGCAATGCTCGATTCAAAAGACGTTGATTCTCAGATCGCTTCTCTCTACGCATTGCTCAATCTTGGAATCGGAAACGATGCGTGA

Protein sequence

>MS.gene060887.t1

MANYHRNNVGNLILGHHHHRSTKTPTFSHHFRLWNSLSIASLRRLIFHAVSCGGATSRYNHRRRSTFNEYDTEDFSSSASSFDKRQQNQTSKPNVKSEKLSDLLNMAEMETEEEAKKKEEALEKLKKLVKDLHEEDDSVKRREAATTVRMLAKENLEVRGTLSMLGAIPPLVAMLDSKDVDSQIASLYALLNLGIGNDA