AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33141.t1 MTR_1g014420 93.246 533 15 4 1 530 1 515 0.0 991
MS.gene33141.t1 MTR_3g106140 63.089 531 159 8 1 530 1 495 0.0 583
MS.gene33141.t1 MTR_2g015870 32.683 410 217 12 125 530 86 440 1.25e-39 149
MS.gene33141.t1 MTR_8g080280 28.793 323 194 7 113 427 415 709 1.71e-29 123
MS.gene33141.t1 MTR_4g063800 31.773 299 166 9 141 433 368 634 9.49e-26 112
MS.gene33141.t1 MTR_3g085610 26.099 364 228 8 77 427 372 707 1.33e-22 102
MS.gene33141.t1 MTR_1g018180 26.110 383 238 12 56 435 3 343 4.43e-22 97.8
MS.gene33141.t1 MTR_4g485520 29.514 288 172 7 142 427 389 647 7.46e-22 99.8
MS.gene33141.t1 MTR_1g069845 30.263 304 181 8 125 427 323 596 1.06e-21 99.4
MS.gene33141.t1 MTR_4g485520 29.514 288 172 7 142 427 221 479 1.45e-21 98.2
MS.gene33141.t1 MTR_1g069845 30.263 304 181 8 125 427 444 717 1.95e-21 98.6
MS.gene33141.t1 MTR_1g069845 30.263 304 181 8 125 427 440 713 2.01e-21 98.6
MS.gene33141.t1 MTR_5g079550 27.586 377 214 10 136 504 30 355 5.61e-21 95.1
MS.gene33141.t1 MTR_3g466220 29.508 305 184 7 124 427 531 805 8.39e-20 93.6
MS.gene33141.t1 MTR_3g466220 29.508 305 184 7 124 427 552 826 1.11e-19 93.2
MS.gene33141.t1 MTR_7g059405 29.514 288 174 5 141 428 376 634 1.94e-19 92.4
MS.gene33141.t1 MTR_7g097020 27.815 302 187 7 127 427 484 755 1.94e-19 92.4
MS.gene33141.t1 MTR_7g095010 68.966 58 18 0 106 163 1 58 9.56e-18 78.2
MS.gene33141.t1 MTR_1g094215 29.766 299 179 6 136 432 172 441 1.58e-17 85.5
MS.gene33141.t1 MTR_7g078330 28.720 289 175 7 141 427 382 641 3.97e-17 85.1
MS.gene33141.t1 MTR_1g094215 29.766 299 179 6 136 432 355 624 5.13e-17 84.7
MS.gene33141.t1 MTR_3g067685 27.569 399 224 13 121 514 44 382 1.75e-16 82.0
MS.gene33141.t1 MTR_5g069340 31.034 290 168 5 143 427 51 313 2.68e-16 80.9
MS.gene33141.t1 MTR_5g083030 30.000 230 132 5 141 367 420 623 5.14e-16 81.6
MS.gene33141.t1 MTR_3g103050 26.398 322 179 10 123 427 49 329 1.48e-15 78.6
MS.gene33141.t1 MTR_2g026130 26.774 310 196 7 120 427 169 449 1.47e-14 76.3
MS.gene33141.t1 MTR_6g004790 25.081 307 197 6 126 427 3 281 3.47e-12 68.2
MS.gene33141.t1 MTR_4g126290 65.625 64 22 0 106 169 1 64 6.87e-12 61.2
MS.gene33141.t1 MTR_2g018010 26.144 306 188 7 144 443 414 687 3.14e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33141.t1 AT4G31890 57.838 555 172 14 1 530 1 518 0.0 543
MS.gene33141.t1 AT4G31890 57.838 555 172 14 1 530 1 518 0.0 543
MS.gene33141.t1 AT4G31890 57.838 555 172 14 1 530 1 518 0.0 543
MS.gene33141.t1 AT2G25130 55.744 531 160 17 1 520 1 467 1.35e-167 483
MS.gene33141.t1 AT2G27430 30.501 459 260 12 78 530 33 438 2.17e-39 149
MS.gene33141.t1 AT1G67530 28.840 319 192 6 122 433 422 712 3.19e-28 119
MS.gene33141.t1 AT1G67530 28.840 319 192 6 122 433 422 712 3.19e-28 119
MS.gene33141.t1 AT1G67530 28.840 319 192 6 122 433 422 712 3.19e-28 119
MS.gene33141.t1 AT1G24330 28.134 359 213 8 109 450 399 729 4.60e-27 116
MS.gene33141.t1 AT1G24330 28.134 359 213 8 109 450 399 729 4.60e-27 116
MS.gene33141.t1 AT2G23140 29.508 366 217 12 65 427 300 627 1.54e-23 105
MS.gene33141.t1 AT2G23140 29.508 366 217 12 65 427 300 627 1.54e-23 105
MS.gene33141.t1 AT3G46510 31.271 291 163 8 141 426 369 627 2.12e-23 104
MS.gene33141.t1 AT2G23140 29.722 360 212 12 71 427 495 816 3.30e-23 104
MS.gene33141.t1 AT2G23140 29.722 360 212 12 71 427 495 816 3.30e-23 104
MS.gene33141.t1 AT2G23140 29.722 360 212 12 71 427 498 819 3.71e-23 104
MS.gene33141.t1 AT2G28830 30.931 333 183 12 112 436 279 572 1.73e-22 101
MS.gene33141.t1 AT1G27910 27.859 341 211 8 94 427 391 703 3.46e-22 101
MS.gene33141.t1 AT1G71020 30.241 291 170 7 141 427 210 471 6.35e-21 96.3
MS.gene33141.t1 AT1G71020 30.241 291 170 7 141 427 358 619 6.66e-21 97.1
MS.gene33141.t1 AT1G23030 30.000 290 171 7 141 427 348 608 9.10e-21 96.3
MS.gene33141.t1 AT3G54790 30.363 303 179 9 127 427 440 712 2.43e-19 92.0
MS.gene33141.t1 AT3G54790 30.363 303 179 9 127 427 476 748 3.64e-19 91.7
MS.gene33141.t1 AT3G54790 30.363 303 179 9 127 427 476 748 3.64e-19 91.7
MS.gene33141.t1 AT5G67340 24.173 393 256 8 36 427 346 697 2.84e-18 89.0
MS.gene33141.t1 AT5G67340 24.173 393 256 8 36 427 346 697 2.84e-18 89.0
MS.gene33141.t1 AT3G01400 26.552 290 182 7 140 427 79 339 3.90e-18 86.7
MS.gene33141.t1 AT3G54850 28.188 298 183 6 140 435 361 629 8.92e-18 87.0
MS.gene33141.t1 AT3G03440 26.174 298 180 8 216 505 138 403 8.03e-16 80.1
MS.gene33141.t1 AT5G58680 27.273 308 188 9 125 427 62 338 2.28e-14 75.1
MS.gene33141.t1 AT5G42340 28.956 297 178 7 140 434 395 660 7.95e-14 74.7
MS.gene33141.t1 AT4G12710 29.153 295 175 6 141 427 62 330 1.84e-12 69.7
MS.gene33141.t1 AT5G62560 26.977 215 145 4 216 427 308 513 7.18e-11 65.1
MS.gene33141.t1 AT4G16490 25.074 339 221 8 93 427 152 461 7.55e-11 64.7

Find 122 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCGAATAAACCGATAAT+TGG 0.146060 1.3:-8501037 MS.gene33141:CDS
AGCCAATGTTCAGTTTATTT+TGG 0.151005 1.3:-8500894 MS.gene33141:CDS
CTTCTTGAGTTGTCGCTTAT+TGG 0.172434 1.3:-8500602 MS.gene33141:CDS
CTGAAGAGTTCTAACTAAAA+AGG 0.175660 1.3:+8500997 None:intergenic
CCTTTCAATCCTTAGCAATT+TGG 0.178700 1.3:-8500810 MS.gene33141:CDS
GACGATGAAGTTCCGCAAAT+TGG 0.211163 1.3:+8500467 None:intergenic
CCCGGATGTGTTTCCTATCT+TGG 0.249308 1.3:-8500750 MS.gene33141:CDS
GGGAACTACAACGGAAATTT+AGG 0.256409 1.3:-8500506 MS.gene33141:CDS
GATAAAGGGAAACAGGTTTC+AGG 0.257229 1.3:-8500527 MS.gene33141:CDS
GTGGGAGAATGTTGGTGTTT+TGG 0.267693 1.3:+8502208 None:intergenic
AATGCTCGCTAAGGAGAATT+TGG 0.287918 1.3:-8501826 MS.gene33141:CDS
TGAGGTTTCCGACGTTGTTC+CGG 0.317043 1.3:+8502258 None:intergenic
GAGAATAGATAAAGGGAAAC+AGG 0.320824 1.3:-8500534 MS.gene33141:CDS
GAAACGGGCGTCGAGGCTAT+TGG 0.335825 1.3:-8500564 MS.gene33141:CDS
GAAAGTTTGAGAATAGATAA+AGG 0.351248 1.3:-8500542 MS.gene33141:CDS
ACGATGAAGTTCCGCAAATT+GGG 0.353654 1.3:+8500468 None:intergenic
GCAGCTGAAGACCCAATTAT+CGG 0.368315 1.3:+8501025 None:intergenic
CTCTACGCATTGCTCAATCT+TGG 0.375202 1.3:-8501714 MS.gene33141:CDS
TCTTGGTCGATGTATTGAAT+TGG 0.380089 1.3:-8500733 MS.gene33141:CDS
TCTCCCACCACTTCCGTCTC+TGG 0.384503 1.3:-8502193 MS.gene33141:CDS
GTTATGTCGCATAAATCTTA+TGG 0.386596 1.3:-8500665 MS.gene33141:CDS
AGTTTCTCCGATTTCACATT+CGG 0.396316 1.3:+8501996 None:intergenic
AAAATAAACTGAACATTGGC+TGG 0.400264 1.3:+8500896 None:intergenic
TGGTGGAACGGTGGTGGTGG+TGG 0.401730 1.3:+8502228 None:intergenic
TTCAAAACCGAATGTGAAAT+CGG 0.408736 1.3:-8502003 MS.gene33141:CDS
AAAGAAGCCATTGTTAAAGT+TGG 0.415455 1.3:-8501160 MS.gene33141:CDS
TTCAATACATCGACCAAGAT+AGG 0.418350 1.3:+8500737 None:intergenic
ATCTTCAGATGGTATTGATT+CGG 0.420177 1.3:-8501107 MS.gene33141:CDS
AAAGTTTGAGAATAGATAAA+GGG 0.425462 1.3:-8500541 MS.gene33141:CDS
AGGAGGAGGAAAAGAGTGTT+CGG 0.438802 1.3:-8500420 MS.gene33141:CDS
TACTACTTTGACTCAGAAAC+GGG 0.441375 1.3:-8500579 MS.gene33141:CDS
ATTCGAATAAACCGATAATT+GGG 0.445804 1.3:-8501036 MS.gene33141:CDS
TCTTCTGAACATGGCGGAGA+TGG 0.446454 1.3:-8501967 MS.gene33141:CDS
GCGGTGATTGTAACGGGAGG+TGG 0.458786 1.3:+8502109 None:intergenic
CCAAATTGCTAAGGATTGAA+AGG 0.463067 1.3:+8500810 None:intergenic
AAGCAGGCCATGATCGAGGC+AGG 0.466247 1.3:-8500638 MS.gene33141:CDS
CCAGATGGAGGAGGAGGAGG+AGG 0.469128 1.3:-8500434 MS.gene33141:CDS
TTCAGTGTCATATTCGTTGA+AGG 0.469707 1.3:+8502076 None:intergenic
TTCACCTGAATGCCAGGAAA+AGG 0.471606 1.3:-8500705 MS.gene33141:CDS
ATGGTTTATTCTCTTCAGAA+TGG 0.471856 1.3:+8500227 None:intergenic
ACGGCGGCGGTGATTGTAAC+GGG 0.473080 1.3:+8502103 None:intergenic
CACAATCCCTGCCTCGATCA+TGG 0.473174 1.3:+8500631 None:intergenic
AAACCAGATGGAGGAGGAGG+AGG 0.478442 1.3:-8500437 MS.gene33141:CDS
GTACTACTTTGACTCAGAAA+CGG 0.478774 1.3:-8500580 MS.gene33141:CDS
TTTGCATGAGGAAGATGATT+CGG 0.481809 1.3:-8501880 MS.gene33141:CDS
ACCAAGATAGGAAACACATC+CGG 0.487266 1.3:+8500749 None:intergenic
ACTGTCTCTGCCCCAATTTG+CGG 0.487673 1.3:-8500479 MS.gene33141:CDS
GAATGATTCACCTGAATGCC+AGG 0.488606 1.3:-8500711 MS.gene33141:CDS
AAGGAGAATTTGGAAGTAAG+AGG 0.491585 1.3:-8501816 MS.gene33141:CDS
ATAAAGGGAAACAGGTTTCA+GGG 0.497964 1.3:-8500526 MS.gene33141:CDS
GGTGGTGGTGGCCGAGAATG+AGG 0.498770 1.3:+8502240 None:intergenic
AGAAGAGGAAGCGAAGAAGA+AGG 0.499264 1.3:-8501940 MS.gene33141:CDS
GCATTGCTCAATCTTGGAAT+CGG 0.503671 1.3:-8501708 MS.gene33141:CDS
CTCCGCCATGTTCAGAAGAT+CGG 0.507404 1.3:+8501971 None:intergenic
AACGTCGGAAACCTCATTCT+CGG 0.508681 1.3:-8502251 MS.gene33141:CDS
AGTTCCAGAGACGGAAGTGG+TGG 0.509557 1.3:+8502189 None:intergenic
ATCTTCCACGCCGTCAGCTG+CGG 0.516615 1.3:-8502137 MS.gene33141:CDS
AAAGGCAATAAGTGCTGTCC+CGG 0.518431 1.3:-8500768 MS.gene33141:CDS
TACAGAACCAACTTTAACAA+TGG 0.525029 1.3:+8501153 None:intergenic
AAGCTTATTGAATCTTCAGA+TGG 0.525195 1.3:-8501118 MS.gene33141:CDS
TTCCACGCCGTCAGCTGCGG+TGG 0.526745 1.3:-8502134 MS.gene33141:CDS
ATCAACTAGAGCAGGTAGAA+AGG 0.527044 1.3:-8500786 MS.gene33141:CDS
GATAAACTCGATTGGCGACA+TGG 0.527711 1.3:-8500849 MS.gene33141:CDS
CGTTGAAGGTGCTACGGCGG+CGG 0.537750 1.3:+8502090 None:intergenic
CAGCTTCCTCCTTCGACAAA+CGG 0.537814 1.3:-8502040 MS.gene33141:CDS
ATTCTGTTGCCGTTTGTCGA+AGG 0.541771 1.3:+8502031 None:intergenic
GAGAGTTCCAGAGACGGAAG+TGG 0.541905 1.3:+8502186 None:intergenic
CGGAAGTGGTGGGAGAATGT+TGG 0.542844 1.3:+8502200 None:intergenic
TTCCAAAATAAACTGAACAT+TGG 0.543173 1.3:+8500892 None:intergenic
CCAAGATAGGAAACACATCC+GGG 0.543957 1.3:+8500750 None:intergenic
GAGACGACGGAGGGAGGCGA+TGG 0.543987 1.3:+8502160 None:intergenic
TAAATCTTATGGTGACAAGC+AGG 0.546845 1.3:-8500654 MS.gene33141:CDS
AATTTGGTATCAACTAGAGC+AGG 0.548849 1.3:-8500794 MS.gene33141:CDS
GAATCGAGCATTGCGACTAA+CGG 0.551485 1.3:+8501765 None:intergenic
TACGGCGGCGGTGATTGTAA+CGG 0.552541 1.3:+8502102 None:intergenic
AGAGGAACACTTTCAATGCT+CGG 0.553240 1.3:-8501798 MS.gene33141:CDS
GGTGGCGCCACCGCAGCTGA+CGG 0.558285 1.3:+8502127 None:intergenic
AGTGACTTAAAATGATCTGA+TGG 0.562549 1.3:+8500278 None:intergenic
CACCGTGAGAATGCTCGCTA+AGG 0.563128 1.3:-8501835 MS.gene33141:CDS
GCAAAACCAGATGGAGGAGG+AGG 0.565741 1.3:-8500440 MS.gene33141:CDS
CTCCTTAGCGAGCATTCTCA+CGG 0.567241 1.3:+8501833 None:intergenic
CGCCACCGCAGCTGACGGCG+TGG 0.567727 1.3:+8502132 None:intergenic
CTGTTGCCGTTTGTCGAAGG+AGG 0.569127 1.3:+8502034 None:intergenic
TCGTCGTGTGCAAAACCAGA+TGG 0.569844 1.3:-8500449 MS.gene33141:CDS
CATGAGGAAAATTGTGAAGA+GGG 0.573352 1.3:-8500324 MS.gene33141:CDS
GAAACTTAAGAAGCTTGTGA+AGG 0.574556 1.3:-8501904 MS.gene33141:CDS
AAATAAACTGAACATTGGCT+GGG 0.578121 1.3:+8500897 None:intergenic
CCAATCGAGTTTATCAGAAA+CGG 0.579904 1.3:+8500857 None:intergenic
TGAGTTTGCAAAACAACATG+AGG 0.582736 1.3:-8500340 MS.gene33141:CDS
CATATTCGTTGAAGGTGCTA+CGG 0.583082 1.3:+8502084 None:intergenic
TAGTTGATACCAAATTGCTA+AGG 0.585434 1.3:+8500801 None:intergenic
GAAGATGAGACGACGGAGGG+AGG 0.594413 1.3:+8502154 None:intergenic
ATAAACTCGATTGGCGACAT+GGG 0.594657 1.3:-8500848 MS.gene33141:CDS
TCGAGCATTGCGACTAACGG+TGG 0.599056 1.3:+8501768 None:intergenic
GGAGAATGGCAAACTATCAC+CGG 0.599573 1.3:-8502277 None:intergenic
CCGTTTCTGATAAACTCGAT+TGG 0.600541 1.3:-8500857 MS.gene33141:CDS
AACTATCACCGGAACAACGT+CGG 0.600978 1.3:-8502266 MS.gene33141:CDS
TTCAGATGGTATTGATTCGG+CGG 0.602952 1.3:-8501104 MS.gene33141:CDS
CGATGAAGTTCCGCAAATTG+GGG 0.609312 1.3:+8500469 None:intergenic
GTTCCAGAGACGGAAGTGGT+GGG 0.614760 1.3:+8502190 None:intergenic
AGGAAGATGATTCGGTGAAG+CGG 0.615812 1.3:-8501872 MS.gene33141:CDS
ACATGAGGAAAATTGTGAAG+AGG 0.616000 1.3:-8500325 MS.gene33141:CDS
GTGACTTAAAATGATCTGAT+GGG 0.620285 1.3:+8500279 None:intergenic
GGCGGAGATGGAGACAGAAG+AGG 0.621626 1.3:-8501955 MS.gene33141:CDS
ACTCTCCGATCTTCTGAACA+TGG 0.624622 1.3:-8501976 MS.gene33141:CDS
TGTGCAAAACCAGATGGAGG+AGG 0.627739 1.3:-8500443 MS.gene33141:CDS
TGGAGAGAGAGTTCCAGAGA+CGG 0.628731 1.3:+8502180 None:intergenic
GTGGAAGATGAGACGACGGA+GGG 0.638181 1.3:+8502151 None:intergenic
GCTTGTGAAGGATTTGCATG+AGG 0.641834 1.3:-8501892 MS.gene33141:CDS
ATTCGTTGAAGGTGCTACGG+CGG 0.652805 1.3:+8502087 None:intergenic
TGACTCAGAAACGGGCGTCG+AGG 0.654081 1.3:-8500571 MS.gene33141:CDS
TTTGTCGAAGGAGGAAGCTG+AGG 0.654659 1.3:+8502043 None:intergenic
AGCAGGCCATGATCGAGGCA+GGG 0.660279 1.3:-8500637 MS.gene33141:CDS
TGACAAGCAGGCCATGATCG+AGG 0.670822 1.3:-8500642 MS.gene33141:CDS
TCTGATGGGACTATATCTTG+AGG 0.670824 1.3:+8500293 None:intergenic
TCGTGTGCAAAACCAGATGG+AGG 0.672294 1.3:-8500446 MS.gene33141:CDS
CGTGGAAGATGAGACGACGG+AGG 0.676052 1.3:+8502150 None:intergenic
CAGGTTTCAGGGAACTACAA+CGG 0.687032 1.3:-8500515 MS.gene33141:CDS
CGGCGTGGAAGATGAGACGA+CGG 0.695582 1.3:+8502147 None:intergenic
GCGGCGGTGATTGTAACGGG+AGG 0.698798 1.3:+8502106 None:intergenic
CTTAGCGAGCATTCTCACGG+TGG 0.717190 1.3:+8501836 None:intergenic
CTCCGATCTTCTGAACATGG+CGG 0.718028 1.3:-8501973 MS.gene33141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTCATTTTTTTTATT+TGG - chr1.3:8501303-8501322 MS.gene33141:intron 10.0%
!!! TTATTAACTTTCTTTTTTTG+TGG - chr1.3:8501004-8501023 MS.gene33141:CDS 15.0%
!! AAAGTTTGAGAATAGATAAA+GGG - chr1.3:8501972-8501991 MS.gene33141:CDS 20.0%
! ATATGTATGATTGTGATGTT+AGG - chr1.3:8501268-8501287 MS.gene33141:intron 25.0%
! ATTCGAATAAACCGATAATT+GGG - chr1.3:8501477-8501496 MS.gene33141:intron 25.0%
! GAAAGTTTGAGAATAGATAA+AGG - chr1.3:8501971-8501990 MS.gene33141:CDS 25.0%
! TTCCAAAATAAACTGAACAT+TGG + chr1.3:8501624-8501643 None:intergenic 25.0%
!! TGAAGAGTTCTAACTAAAAA+GGG + chr1.3:8501518-8501537 None:intergenic 25.0%
!! TTTTTGTGGTTAGGTAATTT+TGG - chr1.3:8501018-8501037 MS.gene33141:CDS 25.0%
!!! AACTTTCTTTTTTTGTGGTT+AGG - chr1.3:8501009-8501028 MS.gene33141:CDS 25.0%
!!! CAAGCATGTGATTTTAATTT+TGG - chr1.3:8501124-8501143 MS.gene33141:CDS 25.0%
!!! GTTTATTTTGGAAACTGATT+TGG - chr1.3:8501631-8501650 MS.gene33141:intron 25.0%
AAAATAAACTGAACATTGGC+TGG + chr1.3:8501620-8501639 None:intergenic 30.0%
AAAGAAGCCATTGTTAAAGT+TGG - chr1.3:8501353-8501372 MS.gene33141:intron 30.0%
AAGCTTATTGAATCTTCAGA+TGG - chr1.3:8501395-8501414 MS.gene33141:intron 30.0%
AGTGACTTAAAATGATCTGA+TGG + chr1.3:8502238-8502257 None:intergenic 30.0%
GATTCGAATAAACCGATAAT+TGG - chr1.3:8501476-8501495 MS.gene33141:intron 30.0%
GTGACTTAAAATGATCTGAT+GGG + chr1.3:8502237-8502256 None:intergenic 30.0%
GTTATGTCGCATAAATCTTA+TGG - chr1.3:8501848-8501867 MS.gene33141:CDS 30.0%
TACAGAACCAACTTTAACAA+TGG + chr1.3:8501363-8501382 None:intergenic 30.0%
TACATACAACTGTAAGATCA+CGG - chr1.3:8500927-8500946 MS.gene33141:CDS 30.0%
TTCAAAACCGAATGTGAAAT+CGG - chr1.3:8500510-8500529 MS.gene33141:CDS 30.0%
! AAATAAACTGAACATTGGCT+GGG + chr1.3:8501619-8501638 None:intergenic 30.0%
! AGCCAATGTTCAGTTTATTT+TGG - chr1.3:8501619-8501638 MS.gene33141:intron 30.0%
! CTGAAGAGTTCTAACTAAAA+AGG + chr1.3:8501519-8501538 None:intergenic 30.0%
! TAGTTGATACCAAATTGCTA+AGG + chr1.3:8501715-8501734 None:intergenic 30.0%
! TTTTCTTGGATTAAGTGCTT+TGG - chr1.3:8501454-8501473 MS.gene33141:intron 30.0%
!! AAAGGTTTCGTATGTTTTGA+TGG - chr1.3:8501826-8501845 MS.gene33141:CDS 30.0%
!! ATCTTCAGATGGTATTGATT+CGG - chr1.3:8501406-8501425 MS.gene33141:intron 30.0%
!!! TGTTGTTTTGCAAACTCAAT+TGG + chr1.3:8502171-8502190 None:intergenic 30.0%
AAGGAGAATTTGGAAGTAAG+AGG - chr1.3:8500697-8500716 MS.gene33141:CDS 35.0%
AATTTGGTATCAACTAGAGC+AGG - chr1.3:8501719-8501738 MS.gene33141:CDS 35.0%
ACATACAACTGTAAGATCAC+GGG - chr1.3:8500928-8500947 MS.gene33141:CDS 35.0%
ACATGAGGAAAATTGTGAAG+AGG - chr1.3:8502188-8502207 MS.gene33141:CDS 35.0%
AGTTTCTCCGATTTCACATT+CGG + chr1.3:8500520-8500539 None:intergenic 35.0%
ATAAAGGGAAACAGGTTTCA+GGG - chr1.3:8501987-8502006 MS.gene33141:CDS 35.0%
CATGAGGAAAATTGTGAAGA+GGG - chr1.3:8502189-8502208 MS.gene33141:CDS 35.0%
CCAAATTGCTAAGGATTGAA+AGG + chr1.3:8501706-8501725 None:intergenic 35.0%
CCAATCGAGTTTATCAGAAA+CGG + chr1.3:8501659-8501678 None:intergenic 35.0%
CCTTTCAATCCTTAGCAATT+TGG - chr1.3:8501703-8501722 MS.gene33141:CDS 35.0%
GAAACTTAAGAAGCTTGTGA+AGG - chr1.3:8500609-8500628 MS.gene33141:CDS 35.0%
GAGAATAGATAAAGGGAAAC+AGG - chr1.3:8501979-8501998 MS.gene33141:CDS 35.0%
GTACTACTTTGACTCAGAAA+CGG - chr1.3:8501933-8501952 MS.gene33141:CDS 35.0%
TAAATCTTATGGTGACAAGC+AGG - chr1.3:8501859-8501878 MS.gene33141:CDS 35.0%
TACTACTTTGACTCAGAAAC+GGG - chr1.3:8501934-8501953 MS.gene33141:CDS 35.0%
TCTTGGTCGATGTATTGAAT+TGG - chr1.3:8501780-8501799 MS.gene33141:CDS 35.0%
TGAGTTTGCAAAACAACATG+AGG - chr1.3:8502173-8502192 MS.gene33141:CDS 35.0%
TTCAATACATCGACCAAGAT+AGG + chr1.3:8501779-8501798 None:intergenic 35.0%
TTCAGTGTCATATTCGTTGA+AGG + chr1.3:8500440-8500459 None:intergenic 35.0%
TTGTGATGTTAGGAGAATAG+TGG - chr1.3:8501278-8501297 MS.gene33141:intron 35.0%
TTTGCATGAGGAAGATGATT+CGG - chr1.3:8500633-8500652 MS.gene33141:CDS 35.0%
! AAACATACGAAACCTTTTCC+TGG + chr1.3:8501823-8501842 None:intergenic 35.0%
! TTGTGGTTAGGTAATTTTGG+TGG - chr1.3:8501021-8501040 MS.gene33141:CDS 35.0%
AATGCTCGCTAAGGAGAATT+TGG - chr1.3:8500687-8500706 MS.gene33141:CDS 40.0%
ACCAAGATAGGAAACACATC+CGG + chr1.3:8501767-8501786 None:intergenic 40.0%
ACGATGAAGTTCCGCAAATT+GGG + chr1.3:8502048-8502067 None:intergenic 40.0%
AGAGGAACACTTTCAATGCT+CGG - chr1.3:8500715-8500734 MS.gene33141:CDS 40.0%
ATAAACTCGATTGGCGACAT+GGG - chr1.3:8501665-8501684 MS.gene33141:intron 40.0%
ATCAACTAGAGCAGGTAGAA+AGG - chr1.3:8501727-8501746 MS.gene33141:CDS 40.0%
CCGTTTCTGATAAACTCGAT+TGG - chr1.3:8501656-8501675 MS.gene33141:intron 40.0%
GATAAAGGGAAACAGGTTTC+AGG - chr1.3:8501986-8502005 MS.gene33141:CDS 40.0%
GCATTGCTCAATCTTGGAAT+CGG - chr1.3:8500805-8500824 MS.gene33141:CDS 40.0%
GGGAACTACAACGGAAATTT+AGG - chr1.3:8502007-8502026 MS.gene33141:CDS 40.0%
TCTGATGGGACTATATCTTG+AGG + chr1.3:8502223-8502242 None:intergenic 40.0%
! CTTCTTGAGTTGTCGCTTAT+TGG - chr1.3:8501911-8501930 MS.gene33141:CDS 40.0%
! GCGATTGTTGCGAATTTTCT+TGG - chr1.3:8501440-8501459 MS.gene33141:intron 40.0%
!! CATATTCGTTGAAGGTGCTA+CGG + chr1.3:8500432-8500451 None:intergenic 40.0%
!! TTCAGATGGTATTGATTCGG+CGG - chr1.3:8501409-8501428 MS.gene33141:intron 40.0%
!!! ATGTTGGTGTTTTGGTGGAA+CGG + chr1.3:8500300-8500319 None:intergenic 40.0%
AACGTCGGAAACCTCATTCT+CGG - chr1.3:8500262-8500281 MS.gene33141:CDS 45.0%
AACTATCACCGGAACAACGT+CGG - chr1.3:8500247-8500266 MS.gene33141:CDS 45.0%
ACTCTCCGATCTTCTGAACA+TGG - chr1.3:8500537-8500556 MS.gene33141:CDS 45.0%
AGAAGAGGAAGCGAAGAAGA+AGG - chr1.3:8500573-8500592 MS.gene33141:CDS 45.0%
CAGGTTTCAGGGAACTACAA+CGG - chr1.3:8501998-8502017 MS.gene33141:CDS 45.0%
CCAAGATAGGAAACACATCC+GGG + chr1.3:8501766-8501785 None:intergenic 45.0%
CGATGAAGTTCCGCAAATTG+GGG + chr1.3:8502047-8502066 None:intergenic 45.0%
CTCTACGCATTGCTCAATCT+TGG - chr1.3:8500799-8500818 MS.gene33141:CDS 45.0%
GAATGATTCACCTGAATGCC+AGG - chr1.3:8501802-8501821 MS.gene33141:CDS 45.0%
GACGATGAAGTTCCGCAAAT+TGG + chr1.3:8502049-8502068 None:intergenic 45.0%
GATAAACTCGATTGGCGACA+TGG - chr1.3:8501664-8501683 MS.gene33141:intron 45.0%
GCAGCTGAAGACCCAATTAT+CGG + chr1.3:8501491-8501510 None:intergenic 45.0%
GCTTGTGAAGGATTTGCATG+AGG - chr1.3:8500621-8500640 MS.gene33141:CDS 45.0%
TTCACCTGAATGCCAGGAAA+AGG - chr1.3:8501808-8501827 MS.gene33141:CDS 45.0%
! AAAGGCAATAAGTGCTGTCC+CGG - chr1.3:8501745-8501764 MS.gene33141:CDS 45.0%
! ATTCTGTTGCCGTTTGTCGA+AGG + chr1.3:8500485-8500504 None:intergenic 45.0%
! GAAACCTTTTCCTGGCATTC+AGG + chr1.3:8501815-8501834 None:intergenic 45.0%
! GAATCGAGCATTGCGACTAA+CGG + chr1.3:8500751-8500770 None:intergenic 45.0%
!! AGGAAGATGATTCGGTGAAG+CGG - chr1.3:8500641-8500660 MS.gene33141:CDS 45.0%
!! AGGAGGAGGAAAAGAGTGTT+CGG - chr1.3:8502093-8502112 MS.gene33141:CDS 45.0%
!! GGAGAATGTTGGTGTTTTGG+TGG + chr1.3:8500305-8500324 None:intergenic 45.0%
!! GTGGGAGAATGTTGGTGTTT+TGG + chr1.3:8500308-8500327 None:intergenic 45.0%
ACTGTCTCTGCCCCAATTTG+CGG - chr1.3:8502034-8502053 MS.gene33141:CDS 50.0%
CAGCTTCCTCCTTCGACAAA+CGG - chr1.3:8500473-8500492 MS.gene33141:CDS 50.0%
CCCGGATGTGTTTCCTATCT+TGG - chr1.3:8501763-8501782 MS.gene33141:CDS 50.0%
CTCCGATCTTCTGAACATGG+CGG - chr1.3:8500540-8500559 MS.gene33141:CDS 50.0%
CTCCGCCATGTTCAGAAGAT+CGG + chr1.3:8500545-8500564 None:intergenic 50.0%
CTCCTTAGCGAGCATTCTCA+CGG + chr1.3:8500683-8500702 None:intergenic 50.0%
TCGTCGTGTGCAAAACCAGA+TGG - chr1.3:8502064-8502083 MS.gene33141:CDS 50.0%
TCGTGTGCAAAACCAGATGG+AGG - chr1.3:8502067-8502086 MS.gene33141:CDS 50.0%
TGAGGTTTCCGACGTTGTTC+CGG + chr1.3:8500258-8500277 None:intergenic 50.0%
TGGAGAGAGAGTTCCAGAGA+CGG + chr1.3:8500336-8500355 None:intergenic 50.0%
TGTGCAAAACCAGATGGAGG+AGG - chr1.3:8502070-8502089 MS.gene33141:CDS 50.0%
TTTGTCGAAGGAGGAAGCTG+AGG + chr1.3:8500473-8500492 None:intergenic 50.0%
! TCTTCTGAACATGGCGGAGA+TGG - chr1.3:8500546-8500565 MS.gene33141:CDS 50.0%
!! ATTCGTTGAAGGTGCTACGG+CGG + chr1.3:8500429-8500448 None:intergenic 50.0%
!!! TTGGTGTTTTGGTGGAACGG+TGG + chr1.3:8500297-8500316 None:intergenic 50.0%
AAACCAGATGGAGGAGGAGG+AGG - chr1.3:8502076-8502095 MS.gene33141:CDS 55.0%
AGTTCCAGAGACGGAAGTGG+TGG + chr1.3:8500327-8500346 None:intergenic 55.0%
CACAATCCCTGCCTCGATCA+TGG + chr1.3:8501885-8501904 None:intergenic 55.0%
CACCGTGAGAATGCTCGCTA+AGG - chr1.3:8500678-8500697 MS.gene33141:CDS 55.0%
CTTAGCGAGCATTCTCACGG+TGG + chr1.3:8500680-8500699 None:intergenic 55.0%
GAGAGTTCCAGAGACGGAAG+TGG + chr1.3:8500330-8500349 None:intergenic 55.0%
GCAAAACCAGATGGAGGAGG+AGG - chr1.3:8502073-8502092 MS.gene33141:CDS 55.0%
GTGGAAGATGAGACGACGGA+GGG + chr1.3:8500365-8500384 None:intergenic 55.0%
GTTCCAGAGACGGAAGTGGT+GGG + chr1.3:8500326-8500345 None:intergenic 55.0%
TACGGCGGCGGTGATTGTAA+CGG + chr1.3:8500414-8500433 None:intergenic 55.0%
TGACAAGCAGGCCATGATCG+AGG - chr1.3:8501871-8501890 MS.gene33141:CDS 55.0%
! CGGAAGTGGTGGGAGAATGT+TGG + chr1.3:8500316-8500335 None:intergenic 55.0%
! CTGTTGCCGTTTGTCGAAGG+AGG + chr1.3:8500482-8500501 None:intergenic 55.0%
! TCGAGCATTGCGACTAACGG+TGG + chr1.3:8500748-8500767 None:intergenic 55.0%
!! TTTTGGTGGAACGGTGGTGG+TGG + chr1.3:8500291-8500310 None:intergenic 55.0%
!!! GTGTTTTGGTGGAACGGTGG+TGG + chr1.3:8500294-8500313 None:intergenic 55.0%
AAGCAGGCCATGATCGAGGC+AGG - chr1.3:8501875-8501894 MS.gene33141:CDS 60.0%
ACGGCGGCGGTGATTGTAAC+GGG + chr1.3:8500413-8500432 None:intergenic 60.0%
AGCAGGCCATGATCGAGGCA+GGG - chr1.3:8501876-8501895 MS.gene33141:CDS 60.0%
ATCTTCCACGCCGTCAGCTG+CGG - chr1.3:8500376-8500395 MS.gene33141:CDS 60.0%
CGGCGTGGAAGATGAGACGA+CGG + chr1.3:8500369-8500388 None:intergenic 60.0%
CGTGGAAGATGAGACGACGG+AGG + chr1.3:8500366-8500385 None:intergenic 60.0%
GAAGATGAGACGACGGAGGG+AGG + chr1.3:8500362-8500381 None:intergenic 60.0%
GGCGGAGATGGAGACAGAAG+AGG - chr1.3:8500558-8500577 MS.gene33141:CDS 60.0%
TCTCCCACCACTTCCGTCTC+TGG - chr1.3:8500320-8500339 MS.gene33141:CDS 60.0%
! GCGGTGATTGTAACGGGAGG+TGG + chr1.3:8500407-8500426 None:intergenic 60.0%
! TGACTCAGAAACGGGCGTCG+AGG - chr1.3:8501942-8501961 MS.gene33141:CDS 60.0%
!! GAAACGGGCGTCGAGGCTAT+TGG - chr1.3:8501949-8501968 MS.gene33141:CDS 60.0%
CCAGATGGAGGAGGAGGAGG+AGG - chr1.3:8502079-8502098 MS.gene33141:CDS 65.0%
CCTCCTCCTCCTCCTCCATC+TGG + chr1.3:8502082-8502101 None:intergenic 65.0%
! GCGGCGGTGATTGTAACGGG+AGG + chr1.3:8500410-8500429 None:intergenic 65.0%
!! CGTTGAAGGTGCTACGGCGG+CGG + chr1.3:8500426-8500445 None:intergenic 65.0%
!! GGTGGTGGTGGCCGAGAATG+AGG + chr1.3:8500276-8500295 None:intergenic 65.0%
!! TGGTGGAACGGTGGTGGTGG+TGG + chr1.3:8500288-8500307 None:intergenic 65.0%
GAGACGACGGAGGGAGGCGA+TGG + chr1.3:8500356-8500375 None:intergenic 70.0%
TTCCACGCCGTCAGCTGCGG+TGG - chr1.3:8500379-8500398 MS.gene33141:CDS 70.0%
GGTGGCGCCACCGCAGCTGA+CGG + chr1.3:8500389-8500408 None:intergenic 75.0%
! CGCCACCGCAGCTGACGGCG+TGG + chr1.3:8500384-8500403 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8500241 8502294 8500241 ID=MS.gene33141
chr1.3 mRNA 8500241 8502294 8500241 ID=MS.gene33141.t1;Parent=MS.gene33141
chr1.3 exon 8501699 8502294 8501699 ID=MS.gene33141.t1.exon1;Parent=MS.gene33141.t1
chr1.3 CDS 8501699 8502294 8501699 ID=cds.MS.gene33141.t1;Parent=MS.gene33141.t1
chr1.3 exon 8500241 8501237 8500241 ID=MS.gene33141.t1.exon2;Parent=MS.gene33141.t1
chr1.3 CDS 8500241 8501237 8500241 ID=cds.MS.gene33141.t1;Parent=MS.gene33141.t1
Gene Sequence

>MS.gene33141

ATGGCAAACTATCACCGGAACAACGTCGGAAACCTCATTCTCGGCCACCACCACCACCGTTCCACCAAAACACCAACATTCTCCCACCACTTCCGTCTCTGGAACTCTCTCTCCATCGCCTCCCTCCGTCGTCTCATCTTCCACGCCGTCAGCTGCGGTGGCGCCACCTCCCGTTACAATCACCGCCGCCGTAGCACCTTCAACGAATATGACACTGAAGACTTCTCTTCCTCAGCTTCCTCCTTCGACAAACGGCAACAGAATCAAACTTCAAAACCGAATGTGAAATCGGAGAAACTCTCCGATCTTCTGAACATGGCGGAGATGGAGACAGAAGAGGAAGCGAAGAAGAAGGAAGAAGCTCTAGAGAAACTTAAGAAGCTTGTGAAGGATTTGCATGAGGAAGATGATTCGGTGAAGCGGCGTGAAGCTGCGACCACCGTGAGAATGCTCGCTAAGGAGAATTTGGAAGTAAGAGGAACACTTTCAATGCTCGGAGCGATTCCACCGTTAGTCGCAATGCTCGATTCAAAAGACGTTGATTCTCAGATCGCTTCTCTCTACGCATTGCTCAATCTTGGAATCGGAAACGATGCGTGAGTTTCTTCATCTTCTTCGCTTTATTTTTATTTTTTTTTTGTTTTTATATGTAGACAAAGTGATAAACATCGCATGAAACTCATATATACATACAACTGTAAGATCACGGGTTCGAAGTCGAGTTTAGCAATATAGACATTGTCAGTTGAATTTGTGTGTTATATTATTAACTTTCTTTTTTTGTGGTTAGGTAATTTTGGTGGTAATTTTGATTGATTATGTCATTTTCATTTAATAATTATTTGATTATGATTATTCTCTTTGTATTAAATTGTTAAAATGACAAGCATGTGATTTTAATTTTGGTAGTTATATATCATTTTTGTTTTTATTATTATTAATAAAGTTGTTCTGTTTTGTAATTTTTTTTAAATGTTAATGTCTATTTCTACACATGATGATGATGTGTGTTTTCATTGTTTCTTGCATATGTATGATTGTGATGTTAGGAGAATAGTGGTAAAAAGTTCATTTTTTTTATTTGGTTTTTTTTTTTTTGTTATTACAGAAATAAAGAAGCCATTGTTAAAGTTGGTTCTGTACACAAGATGCTTAAGCTTATTGAATCTTCAGATGGTATTGATTCGGCGGTTTCTGAAGCGATTGTTGCGAATTTTCTTGGATTAAGTGCTTTGGATTCGAATAAACCGATAATTGGGTCTTCAGCTGCAATACCCTTTTTAGTTAGAACTCTTCAGAATAAGAATTTAGATAAGAAAAGTAGCAATCAAGTTAAGCAAGATGCTCTTAGAGCACTTTACAATCTTTCAATCTTCCCAGCCAATGTTCAGTTTATTTTGGAAACTGATTTGGTACCGTTTCTGATAAACTCGATTGGCGACATGGGAGTAACTGAAAGAAACCTTTCAATCCTTAGCAATTTGGTATCAACTAGAGCAGGTAGAAAGGCAATAAGTGCTGTCCCGGATGTGTTTCCTATCTTGGTCGATGTATTGAATTGGAATGATTCACCTGAATGCCAGGAAAAGGTTTCGTATGTTTTGATGGTTATGTCGCATAAATCTTATGGTGACAAGCAGGCCATGATCGAGGCAGGGATTGTGTCGTCGCTTCTTGAGTTGTCGCTTATTGGTACTACTTTGACTCAGAAACGGGCGTCGAGGCTATTGGAAAGTTTGAGAATAGATAAAGGGAAACAGGTTTCAGGGAACTACAACGGAAATTTAGGTGCAACTGTCTCTGCCCCAATTTGCGGAACTTCATCGTCGTGTGCAAAACCAGATGGAGGAGGAGGAGGAGGAAAAGAGTGTTCGGAAGAAGATGAAGATATGATGAGCGAAGAGAAGAAAGCAGTGAAGCAATTAGTCCAATTGAGTTTGCAAAACAACATGAGGAAAATTGTGAAGAGGGCGAACTTGCCTCAAGATATAGTCCCATCAGATCATTTTAAGTCACTCACTTCAAGTTCAACTTCAAAGAGTTTACCATTCTGA
Protein sequence

>MS.gene33141.t1

MANYHRNNVGNLILGHHHHRSTKTPTFSHHFRLWNSLSIASLRRLIFHAVSCGGATSRYNHRRRSTFNEYDTEDFSSSASSFDKRQQNQTSKPNVKSEKLSDLLNMAEMETEEEAKKKEEALEKLKKLVKDLHEEDDSVKRREAATTVRMLAKENLEVRGTLSMLGAIPPLVAMLDSKDVDSQIASLYALLNLGIGNDAGKKFIFFIWFFFFCYYRNKEAIVKVGSVHKMLKLIESSDGIDSAVSEAIVANFLGLSALDSNKPIIGSSAAIPFLVRTLQNKNLDKKSSNQVKQDALRALYNLSIFPANVQFILETDLVPFLINSIGDMGVTERNLSILSNLVSTRAGRKAISAVPDVFPILVDVLNWNDSPECQEKVSYVLMVMSHKSYGDKQAMIEAGIVSSLLELSLIGTTLTQKRASRLLESLRIDKGKQVSGNYNGNLGATVSAPICGTSSSCAKPDGGGGGGKECSEEDEDMMSEEKKAVKQLVQLSLQNNMRKIVKRANLPQDIVPSDHFKSLTSSSTSKSLPF