Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061061.t1 | XP_013466698.1 | 98.8 | 408 | 4 | 1 | 10 | 416 | 52 | 459 | 5.60E-219 | 770 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061061.t1 | Q8BNU0 | 23.7 | 338 | 234 | 10 | 3 | 330 | 47 | 370 | 6.9e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061061.t1 | A0A072VFC8 | 98.8 | 408 | 4 | 1 | 10 | 416 | 52 | 459 | 4.0e-219 | 770.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061061 | MS.gene75473 | 0.817481 | 3.23E-52 | -1.69E-46 |
| MS.gene061061 | MS.gene79219 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene061061 | MS.gene79892 | 0.80145 | 8.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061061.t1 | MTR_1g034000 | 98.775 | 408 | 4 | 1 | 10 | 416 | 52 | 459 | 0.0 | 808 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061061.t1 | AT4G33945 | 59.712 | 417 | 157 | 2 | 10 | 415 | 47 | 463 | 2.23e-165 | 473 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCATGCATCACTTTAA+AGG | 0.196359 | 1.4:-18831373 | MS.gene061061:CDS |
| TCTCGAGTTGTTCTTGTTTC+GGG | 0.198609 | 1.4:-18833300 | MS.gene061061:CDS |
| TACTGATGCCTTGAGGGATT+TGG | 0.206714 | 1.4:-18823940 | MS.gene061061:CDS |
| GATTGTCAAAGACTCATTTA+TGG | 0.229944 | 1.4:-18831818 | MS.gene061061:CDS |
| ACTGATGCCTTGAGGGATTT+GGG | 0.237834 | 1.4:-18823939 | MS.gene061061:CDS |
| ATCTCGAGTTGTTCTTGTTT+CGG | 0.256717 | 1.4:-18833301 | MS.gene061061:CDS |
| TGTAAATCCATTGCTGAAAA+AGG | 0.271503 | 1.4:-18830607 | MS.gene061061:CDS |
| TCCTTGGTCCATTACTGTTC+TGG | 0.297839 | 1.4:+18831932 | None:intergenic |
| AGTAGGATCGTCAGCATATC+TGG | 0.316368 | 1.4:+18826381 | None:intergenic |
| ATCGCACGCGCCGTCGTCTT+TGG | 0.318375 | 1.4:+18833796 | None:intergenic |
| GACAGGAGTTTCTTTGCATT+TGG | 0.340475 | 1.4:+18824375 | None:intergenic |
| AATGGACCAAGGATTATAAT+TGG | 0.343523 | 1.4:-18831922 | MS.gene061061:CDS |
| TTATGAACTTGCCTGTTCTC+TGG | 0.343779 | 1.4:+18824323 | None:intergenic |
| GCCTTGGCGTTGTTGCTTCA+TGG | 0.344647 | 1.4:-18833270 | MS.gene061061:intron |
| GGTATCGTAACGTCGCTTCC+TGG | 0.371668 | 1.4:-18833528 | MS.gene061061:CDS |
| AGAACTATTCTGCTTAATAA+TGG | 0.380349 | 1.4:-18824020 | MS.gene061061:CDS |
| CTTTGCATTTGGTGCGCTGC+AGG | 0.382774 | 1.4:+18824386 | None:intergenic |
| TGTCTTAAATAGTAGCTTCC+GGG | 0.389360 | 1.4:-18831869 | MS.gene061061:CDS |
| GGCATCTGTAATTCCAATCT+TGG | 0.398403 | 1.4:+18831440 | None:intergenic |
| GCACGAAAATTCGCCAAGAT+TGG | 0.408368 | 1.4:-18831453 | MS.gene061061:CDS |
| ATGTCTTAAATAGTAGCTTC+CGG | 0.408648 | 1.4:-18831870 | MS.gene061061:CDS |
| AAATAGTAGCTTCCGGGTTG+TGG | 0.409052 | 1.4:-18831863 | MS.gene061061:CDS |
| ATGATACCAATTATAATCCT+TGG | 0.413217 | 1.4:+18831916 | None:intergenic |
| GGAGGTGTTGAGTTGGTTTG+TGG | 0.430247 | 1.4:-18833354 | MS.gene061061:CDS |
| CACTGTCATCTATACATTTG+AGG | 0.430334 | 1.4:+18830569 | None:intergenic |
| GTCACCAGCACCAGCTTCAA+TGG | 0.448466 | 1.4:+18824436 | None:intergenic |
| TAGCGAGAAACCCAGAGAAC+AGG | 0.452111 | 1.4:-18824334 | MS.gene061061:intron |
| TCATATAAACTTTGAATGCC+AGG | 0.453747 | 1.4:+18831745 | None:intergenic |
| TATGAACTTGCCTGTTCTCT+GGG | 0.459958 | 1.4:+18824324 | None:intergenic |
| GTTGTGGCTTCAGCTGCGAC+TGG | 0.474835 | 1.4:-18831847 | MS.gene061061:CDS |
| GTGGTGTTTGTGAAAAGATA+AGG | 0.480094 | 1.4:-18833335 | MS.gene061061:CDS |
| TTGAGTTGTGTGGTGAAGAT+GGG | 0.480693 | 1.4:-18833407 | MS.gene061061:CDS |
| GTTGAGTTGTGTGGTGAAGA+TGG | 0.483302 | 1.4:-18833408 | MS.gene061061:CDS |
| TGTTTCGGGTTTGAATGCCT+TGG | 0.483439 | 1.4:-18833286 | MS.gene061061:CDS |
| GATTACATTGGATGAGATTG+TGG | 0.486167 | 1.4:-18833448 | MS.gene061061:CDS |
| GCAAACAAAAGTGCAATTGT+TGG | 0.487710 | 1.4:-18826439 | MS.gene061061:CDS |
| AAGTAATGAGCAACAAACTT+TGG | 0.489076 | 1.4:+18830522 | None:intergenic |
| AAAGTGCAATTGTTGGGAAA+GGG | 0.490046 | 1.4:-18826432 | MS.gene061061:CDS |
| TGTTTAATTGATCAGCTAGC+AGG | 0.494220 | 1.4:-18826469 | MS.gene061061:intron |
| AGCTGCTACTGATGCCTTGA+GGG | 0.495243 | 1.4:-18823946 | MS.gene061061:CDS |
| TGAGAATGAAGAGATTACAT+TGG | 0.495980 | 1.4:-18833460 | MS.gene061061:CDS |
| GCAGCCCGTGCCATTGAAGC+TGG | 0.499725 | 1.4:-18824446 | MS.gene061061:CDS |
| TTGTTGCTCATTACTTTCAA+AGG | 0.500260 | 1.4:-18830515 | MS.gene061061:intron |
| CTGATGCCTTGAGGGATTTG+GGG | 0.501997 | 1.4:-18823938 | MS.gene061061:CDS |
| CAAACAAAAGTGCAATTGTT+GGG | 0.511346 | 1.4:-18826438 | MS.gene061061:CDS |
| AAGTGATGCATGCTGAAACT+AGG | 0.513315 | 1.4:+18831379 | None:intergenic |
| GCTAGAACACGATTGTCATC+AGG | 0.517264 | 1.4:+18831700 | None:intergenic |
| TTCACTTACATTTACTGCAG+TGG | 0.518196 | 1.4:+18831350 | None:intergenic |
| CGTGCCATTGAAGCTGGTGC+TGG | 0.518350 | 1.4:-18824440 | MS.gene061061:CDS |
| CGCTGCAGGAAATTTCTGCA+TGG | 0.522525 | 1.4:+18824400 | None:intergenic |
| AATGCCAGGGTTCTTATGTA+CGG | 0.525210 | 1.4:+18831759 | None:intergenic |
| TAGATTTCTGATCATAAGAC+AGG | 0.528394 | 1.4:+18824358 | None:intergenic |
| GCTGCAGATGTTCAAAGTAC+AGG | 0.530167 | 1.4:-18831961 | MS.gene061061:intron |
| AGTGTTTAGAGAAGCTGAAA+CGG | 0.535318 | 1.4:-18833485 | MS.gene061061:CDS |
| AGCACCAGCTTCAATGGCAC+GGG | 0.537795 | 1.4:+18824442 | None:intergenic |
| TTCTCTAAACACTCTATCAC+CGG | 0.538634 | 1.4:+18833495 | None:intergenic |
| ATGGAATTGAGAAACACATA+AGG | 0.538760 | 1.4:-18824001 | MS.gene061061:CDS |
| TTGAGGGATTTGGGGCTCGA+TGG | 0.539940 | 1.4:-18823930 | MS.gene061061:CDS |
| AGTAGCAGCTTCCTTGCAGT+TGG | 0.544576 | 1.4:+18823959 | None:intergenic |
| AAAAGTGCAATTGTTGGGAA+AGG | 0.546574 | 1.4:-18826433 | MS.gene061061:CDS |
| GAAAGTTGATGAGCTAATTG+TGG | 0.552556 | 1.4:-18831791 | MS.gene061061:CDS |
| CTTACCTCTTGCAAAACAGT+AGG | 0.555591 | 1.4:+18826364 | None:intergenic |
| GGAACATTCCAGAACAGTAA+TGG | 0.556108 | 1.4:-18831940 | MS.gene061061:CDS |
| CATATAAACTTTGAATGCCA+GGG | 0.556778 | 1.4:+18831746 | None:intergenic |
| TGGAATTACAGATGCCCTTG+TGG | 0.558440 | 1.4:-18831433 | MS.gene061061:CDS |
| CAGCACCAGCTTCAATGGCA+CGG | 0.560837 | 1.4:+18824441 | None:intergenic |
| ATCACCGGATTACTCTCTCC+AGG | 0.561725 | 1.4:+18833510 | None:intergenic |
| GAAAAGATAAGGAGTAGTGA+TGG | 0.563483 | 1.4:-18833324 | MS.gene061061:CDS |
| GATAAACTTGTTGAGTTGTG+TGG | 0.564969 | 1.4:-18833417 | MS.gene061061:CDS |
| AAGTGCAATTGTTGGGAAAG+GGG | 0.570180 | 1.4:-18826431 | MS.gene061061:CDS |
| AAGCTGCTACTGATGCCTTG+AGG | 0.606218 | 1.4:-18823947 | MS.gene061061:CDS |
| GCTTCCTGGAGAGAGTAATC+CGG | 0.606746 | 1.4:-18833514 | MS.gene061061:CDS |
| TCGCAGCTGAAGCCACAACC+CGG | 0.607749 | 1.4:+18831851 | None:intergenic |
| AGTGCAATTGTTGGGAAAGG+GGG | 0.613873 | 1.4:-18826430 | MS.gene061061:CDS |
| ATGCATGCTGAAACTAGGTC+AGG | 0.618044 | 1.4:+18831384 | None:intergenic |
| ATAGATGACAGTGGTGAACA+AGG | 0.624512 | 1.4:-18830559 | MS.gene061061:CDS |
| TCTGCATGGCTTGAATAGCA+AGG | 0.626175 | 1.4:+18824414 | None:intergenic |
| ATCGAGCCCCAAATCCCTCA+AGG | 0.626177 | 1.4:+18823932 | None:intergenic |
| ACCATGAAGCAACAACGCCA+AGG | 0.633556 | 1.4:+18833269 | None:intergenic |
| GCAAACACATGCCAACTGCA+AGG | 0.634812 | 1.4:-18823970 | MS.gene061061:CDS |
| CTGTGTGAAGAGAATCCACA+AGG | 0.637406 | 1.4:+18831418 | None:intergenic |
| CTTGTGGATTCTCTTCACAC+AGG | 0.643028 | 1.4:-18831417 | MS.gene061061:CDS |
| TTGTGTGGTGAAGATGGGAA+TGG | 0.657286 | 1.4:-18833402 | MS.gene061061:CDS |
| ATGTCCGTACATAAGAACCC+TGG | 0.658108 | 1.4:-18831763 | MS.gene061061:CDS |
| TGAACAAGGCAACACAGATG+TGG | 0.665026 | 1.4:-18830545 | MS.gene061061:CDS |
| TCCAGAACAGTAATGGACCA+AGG | 0.679782 | 1.4:-18831933 | MS.gene061061:CDS |
| CTCAAATGTATAGATGACAG+TGG | 0.716567 | 1.4:-18830568 | MS.gene061061:CDS |
| AATGGGTGTACCAAAGACGA+CGG | 0.730349 | 1.4:-18833806 | MS.gene061061:CDS |
| TGTGTGAAGAGAATCCACAA+GGG | 0.777922 | 1.4:+18831419 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 18823917 | 18833827 | 18823917 | ID=MS.gene061061 |
| chr1.4 | mRNA | 18823917 | 18833827 | 18823917 | ID=MS.gene061061.t1;Parent=MS.gene061061 |
| chr1.4 | exon | 18833803 | 18833827 | 18833803 | ID=MS.gene061061.t1.exon1;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18833803 | 18833827 | 18833803 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18833271 | 18833549 | 18833271 | ID=MS.gene061061.t1.exon2;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18833271 | 18833549 | 18833271 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18831693 | 18831976 | 18831693 | ID=MS.gene061061.t1.exon3;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18831693 | 18831976 | 18831693 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18831359 | 18831487 | 18831359 | ID=MS.gene061061.t1.exon4;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18831359 | 18831487 | 18831359 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18830516 | 18830638 | 18830516 | ID=MS.gene061061.t1.exon5;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18830516 | 18830638 | 18830516 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18826369 | 18826476 | 18826369 | ID=MS.gene061061.t1.exon6;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18826369 | 18826476 | 18826369 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18824335 | 18824513 | 18824335 | ID=MS.gene061061.t1.exon7;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18824335 | 18824513 | 18824335 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| chr1.4 | exon | 18823917 | 18824040 | 18823917 | ID=MS.gene061061.t1.exon8;Parent=MS.gene061061.t1 |
| chr1.4 | CDS | 18823917 | 18824040 | 18823917 | ID=cds.MS.gene061061.t1;Parent=MS.gene061061.t1 |
| Gene Sequence |
| Protein sequence |
