AlfalfaGEDB | MS.gene061499

M. sativa cultivar XinJiangDaYe / MS.gene061499

KEGG: K02357
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0005488 GO:0005515 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:1901363 GO:1901576
PFAM: PF00889.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061499.t1	XP_003620654.1	94.2	208	12	0	5	212	844	1051	1.10E-98	369.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061499.t1	A3PCG2	61.2	206	79	1	11	215	4	209	1.3e-66	254.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061499.t1	G7KPU0	94.2	208	12	0	5	212	844	1051	8.2e-99	369.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050226	MS.gene061499	0.813607	2.35E-51	-1.69E-46
MS.gene050227	MS.gene061499	0.824233	9.02E-54	-1.69E-46
MS.gene050228	MS.gene061499	0.82276	1.99E-53	-1.69E-46
MS.gene050442	MS.gene061499	0.825006	5.93E-54	-1.69E-46
MS.gene050719	MS.gene061499	0.822496	2.30E-53	-1.69E-46
MS.gene050721	MS.gene061499	0.823815	1.13E-53	-1.69E-46
MS.gene050724	MS.gene061499	0.807518	4.86E-50	-1.69E-46
MS.gene050780	MS.gene061499	0.80038	1.48E-48	-1.69E-46
MS.gene050959	MS.gene061499	0.800856	1.18E-48	-1.69E-46
MS.gene052020	MS.gene061499	0.825573	4.35E-54	-1.69E-46
MS.gene052896	MS.gene061499	0.811812	5.81E-51	-1.69E-46
MS.gene053221	MS.gene061499	0.804334	2.27E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061499.t1	MTR_6g088270	94.231	208	12	0	5	212	844	1051	3.46e-132	397
MS.gene061499.t1	MTR_6g088270	66.507	209	70	0	7	215	605	813	1.18e-93	295
MS.gene061499.t1	MTR_2g039780	74.251	167	27	3	47	212	62	213	3.75e-75	226
MS.gene061499.t1	MTR_6g087300	66.310	187	34	1	1	187	200	357	1.38e-71	222
MS.gene061499.t1	MTR_6g087300	38.312	154	49	1	52	205	50	157	1.96e-26	104
MS.gene061499.t1	MTR_6g084360	72.892	166	16	1	42	207	973	1109	1.84e-69	229
MS.gene061499.t1	MTR_0428s0020	57.576	198	29	3	1	198	34	176	8.57e-58	182
MS.gene061499.t1	MTR_2g039790	77.391	115	13	1	1	115	11	112	9.92e-55	172
MS.gene061499.t1	MTR_6g088240	53.723	188	70	3	28	203	1	183	6.60e-54	171
MS.gene061499.t1	MTR_6g488290	56.216	185	26	3	1	185	34	163	4.09e-52	166
MS.gene061499.t1	MTR_6g027070	54.902	153	50	2	8	160	10	143	3.02e-47	153
MS.gene061499.t1	MTR_6g088250	78.261	92	19	1	2	92	1251	1342	1.47e-38	141
MS.gene061499.t1	MTR_6g066260	48.421	95	49	0	39	133	3	97	4.00e-24	92.0
MS.gene061499.t1	MTR_6g087200	50.000	82	31	1	71	152	1033	1104	5.27e-20	88.2
MS.gene061499.t1	MTR_6g003970	46.237	93	49	1	15	107	31	122	4.56e-16	75.9
MS.gene061499.t1	MTR_6g087190	76.923	39	9	0	59	97	15	53	2.30e-15	68.6
MS.gene061499.t1	MTR_6g081090	79.070	43	9	0	1	43	1	43	3.14e-15	69.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061499.t1	AT4G29060	82.741	197	34	0	9	205	755	951	2.85e-108	332
MS.gene061499.t1	AT4G29060	71.078	204	59	0	11	214	519	722	1.95e-97	303
MS.gene061499.t1	AT4G29060	74.872	195	43	1	11	205	519	707	1.19e-97	299
MS.gene061499.t1	AT4G11120	47.312	93	46	2	14	106	76	165	1.95e-15	74.3

Find 40 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAGCATACCTCAGGAAAA+AGG	0.285723	6.1:-76997589	MS.gene061499:CDS
ACACTGTCATCCTTAATAAA+AGG	0.287447	6.1:+76997216	None:intergenic
CTAGCAGTTGAAGGAAGAAT+TGG	0.407868	6.1:-76997534	MS.gene061499:CDS
ACTTGTAAGCTATGCTAGTT+CGG	0.428433	6.1:+76997086	None:intergenic
TCATCCGCTGATAAGAAATC+TGG	0.434261	6.1:-76997561	MS.gene061499:CDS
ACTTTAATGTTCTCTCCAAT+AGG	0.442489	6.1:+76997159	None:intergenic
TACATTCACGATGCACGTAT+TGG	0.457289	6.1:-76997507	MS.gene061499:CDS
GGAAGAATCTCAAAGAGGTT+AGG	0.459157	6.1:-76997261	MS.gene061499:CDS
AGAAATCGGTGCAGGGATGA+TGG	0.461673	6.1:-76997658	MS.gene061499:CDS
AGGCAAAGTATAGCTCCTAT+TGG	0.461869	6.1:-76997174	MS.gene061499:CDS
AGTAGTGGTCTCAGCCGCTT+TGG	0.466995	6.1:-76997697	MS.gene061499:CDS
ATTGTTCAATGTTGTGGTGC+AGG	0.476127	6.1:-76997728	None:intergenic
GCAAGTTGCTGCTTGTCCAC+AGG	0.499547	6.1:-76997403	MS.gene061499:CDS
TCTGGAAGACTAGCAGTTGA+AGG	0.500080	6.1:-76997543	MS.gene061499:CDS
AACTGAAGGTGATCTTGAAA+AGG	0.502779	6.1:-76997613	MS.gene061499:CDS
CTCACTTCTGCCAACAAAGT+CGG	0.506846	6.1:+76997458	None:intergenic
AAGCAACTACGAGAAGAAAT+CGG	0.523154	6.1:-76997672	MS.gene061499:CDS
TGGCAGAAGTGAGAAATTCA+AGG	0.527151	6.1:-76997448	MS.gene061499:CDS
AAGTGAGAAATTCAAGGAGT+TGG	0.528224	6.1:-76997442	MS.gene061499:CDS
CTACGAGAAGAAATCGGTGC+AGG	0.531943	6.1:-76997666	MS.gene061499:CDS
TGTTCTCGGGTTTCGAAGCA+AGG	0.537039	6.1:+76997304	None:intergenic
TATTTAATTGTTCAATGTTG+TGG	0.541844	6.1:-76997734	None:intergenic
AACTGTGAAACCGACTTTGT+TGG	0.542867	6.1:-76997468	MS.gene061499:CDS
AAAAGGCACAAGCATACCTC+AGG	0.555573	6.1:-76997596	MS.gene061499:CDS
ATCACCTTCAGTTTCAGCAA+GGG	0.556148	6.1:+76997623	None:intergenic
ATTTCTTATCAGCGGATGAG+AGG	0.576762	6.1:+76997565	None:intergenic
TCTTCCAGATTTCTTATCAG+CGG	0.576941	6.1:+76997557	None:intergenic
GTGCAGGAAATCAGCAGTAG+TGG	0.596207	6.1:-76997712	MS.gene061499:CDS
TACGAGAAGAAATCGGTGCA+GGG	0.608538	6.1:-76997665	MS.gene061499:CDS
AAAGCCCTTGCTGAAACTGA+AGG	0.615327	6.1:-76997627	MS.gene061499:CDS
TATCGAAACTATTGTGACGA+AGG	0.617946	6.1:-76997355	MS.gene061499:CDS
GATCACCTTCAGTTTCAGCA+AGG	0.621026	6.1:+76997622	None:intergenic
CATTAGAGAGAAAATTGTCG+AGG	0.623126	6.1:-76997283	MS.gene061499:CDS
ATAGATATAAACTGAACCTG+TGG	0.628108	6.1:+76997387	None:intergenic
ATTAGAGAGAAAATTGTCGA+GGG	0.630225	6.1:-76997282	MS.gene061499:CDS
TCGTAGTTGCTTAACCAAAG+CGG	0.653913	6.1:+76997683	None:intergenic
CAGTGTTGTAGTGAAAGACT+TGG	0.668834	6.1:-76997199	MS.gene061499:CDS
TCGAGGGAAGAATCTCAAAG+AGG	0.687349	6.1:-76997266	MS.gene061499:CDS
TTGTAGTGAAAGACTTGGTG+AGG	0.687704	6.1:-76997194	MS.gene061499:CDS
TTGGAGAGAACATTAAAGTG+CGG	0.701032	6.1:-76997155	MS.gene061499:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTTCTCTCTAATGTTCT+CGG	+	chr6.1:76997528-76997547	None:intergenic	25.0%
	ACACTGTCATCCTTAATAAA+AGG	+	chr6.1:76997602-76997621	None:intergenic	30.0%
	ACTTTAATGTTCTCTCCAAT+AGG	+	chr6.1:76997659-76997678	None:intergenic	30.0%
	ATAGATATAAACTGAACCTG+TGG	+	chr6.1:76997431-76997450	None:intergenic	30.0%
	ATTAGAGAGAAAATTGTCGA+GGG	-	chr6.1:76997533-76997552	MS.gene061499:CDS	30.0%
!	ATTTTCTCTCTAATGTTCTC+GGG	+	chr6.1:76997527-76997546	None:intergenic	30.0%
!	TCTAGAGATGCCTTTTATTA+AGG	-	chr6.1:76997589-76997608	MS.gene061499:CDS	30.0%
	AACTGAAGGTGATCTTGAAA+AGG	-	chr6.1:76997202-76997221	MS.gene061499:CDS	35.0%
	AAGCAACTACGAGAAGAAAT+CGG	-	chr6.1:76997143-76997162	MS.gene061499:CDS	35.0%
	AAGTGAGAAATTCAAGGAGT+TGG	-	chr6.1:76997373-76997392	MS.gene061499:CDS	35.0%
	CATTAGAGAGAAAATTGTCG+AGG	-	chr6.1:76997532-76997551	MS.gene061499:CDS	35.0%
	TATCGAAACTATTGTGACGA+AGG	-	chr6.1:76997460-76997479	MS.gene061499:CDS	35.0%
	TCTTCCAGATTTCTTATCAG+CGG	+	chr6.1:76997261-76997280	None:intergenic	35.0%
	TTGGAGAGAACATTAAAGTG+CGG	-	chr6.1:76997660-76997679	MS.gene061499:CDS	35.0%
	ACAAGCATACCTCAGGAAAA+AGG	-	chr6.1:76997226-76997245	MS.gene061499:CDS	40.0%
	AGGCAAAGTATAGCTCCTAT+TGG	-	chr6.1:76997641-76997660	MS.gene061499:CDS	40.0%
	ATCACCTTCAGTTTCAGCAA+GGG	+	chr6.1:76997195-76997214	None:intergenic	40.0%
	ATTTCTTATCAGCGGATGAG+AGG	+	chr6.1:76997253-76997272	None:intergenic	40.0%
	CAAGCATACCTCAGGAAAAA+GGG	-	chr6.1:76997227-76997246	MS.gene061499:CDS	40.0%
	CAGTGTTGTAGTGAAAGACT+TGG	-	chr6.1:76997616-76997635	MS.gene061499:CDS	40.0%
	CTAGCAGTTGAAGGAAGAAT+TGG	-	chr6.1:76997281-76997300	MS.gene061499:CDS	40.0%
	GGAAGAATCTCAAAGAGGTT+AGG	-	chr6.1:76997554-76997573	MS.gene061499:CDS	40.0%
	TACATTCACGATGCACGTAT+TGG	-	chr6.1:76997308-76997327	MS.gene061499:CDS	40.0%
	TCATCCGCTGATAAGAAATC+TGG	-	chr6.1:76997254-76997273	MS.gene061499:CDS	40.0%
	TCGTAGTTGCTTAACCAAAG+CGG	+	chr6.1:76997135-76997154	None:intergenic	40.0%
	TGGCAGAAGTGAGAAATTCA+AGG	-	chr6.1:76997367-76997386	MS.gene061499:CDS	40.0%
!	AACTGTGAAACCGACTTTGT+TGG	-	chr6.1:76997347-76997366	MS.gene061499:CDS	40.0%
!	TTAAAGTGCGGCGTTTTCTT+CGG	-	chr6.1:76997672-76997691	MS.gene061499:CDS	40.0%
!!	TTGTAGTGAAAGACTTGGTG+AGG	-	chr6.1:76997621-76997640	MS.gene061499:CDS	40.0%
	AAAGCCCTTGCTGAAACTGA+AGG	-	chr6.1:76997188-76997207	MS.gene061499:CDS	45.0%
	CTCACTTCTGCCAACAAAGT+CGG	+	chr6.1:76997360-76997379	None:intergenic	45.0%
	GATCACCTTCAGTTTCAGCA+AGG	+	chr6.1:76997196-76997215	None:intergenic	45.0%
	TACGAGAAGAAATCGGTGCA+GGG	-	chr6.1:76997150-76997169	MS.gene061499:CDS	45.0%
	TCGAGGGAAGAATCTCAAAG+AGG	-	chr6.1:76997549-76997568	MS.gene061499:CDS	45.0%
	TCTGGAAGACTAGCAGTTGA+AGG	-	chr6.1:76997272-76997291	MS.gene061499:CDS	45.0%
!	AAAAGGCACAAGCATACCTC+AGG	-	chr6.1:76997219-76997238	MS.gene061499:CDS	45.0%
!	CGTTTTCTTCGGTTCACTCT+CGG	-	chr6.1:76997683-76997702	MS.gene061499:CDS	45.0%
	AGAAATCGGTGCAGGGATGA+TGG	-	chr6.1:76997157-76997176	MS.gene061499:CDS	50.0%
	CTACGAGAAGAAATCGGTGC+AGG	-	chr6.1:76997149-76997168	MS.gene061499:CDS	50.0%
	GTGCAGGAAATCAGCAGTAG+TGG	-	chr6.1:76997103-76997122	MS.gene061499:CDS	50.0%
	TGTTCTCGGGTTTCGAAGCA+AGG	+	chr6.1:76997514-76997533	None:intergenic	50.0%
!	ATGAGAGGCCCTTTTTCCTG+AGG	+	chr6.1:76997238-76997257	None:intergenic	50.0%
	AGTAGTGGTCTCAGCCGCTT+TGG	-	chr6.1:76997118-76997137	MS.gene061499:CDS	55.0%
!	GCAAGTTGCTGCTTGTCCAC+AGG	-	chr6.1:76997412-76997431	MS.gene061499:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	76997095	76997742	76997095	ID=MS.gene061499
chr6.1	mRNA	76997095	76997742	76997095	ID=MS.gene061499.t1;Parent=MS.gene061499
chr6.1	exon	76997095	76997742	76997095	ID=MS.gene061499.t1.exon1;Parent=MS.gene061499.t1
chr6.1	CDS	76997095	76997742	76997095	ID=cds.MS.gene061499.t1;Parent=MS.gene061499.t1

Gene Sequence

>MS.gene061499

ATGTTGTGGTGCAGGAAATCAGCAGTAGTGGTCTCAGCCGCTTTGGTTAAGCAACTACGAGAAGAAATCGGTGCAGGGATGATGGAATGTAAGAAAGCCCTTGCTGAAACTGAAGGTGATCTTGAAAAGGCACAAGCATACCTCAGGAAAAAGGGCCTCTCATCCGCTGATAAGAAATCTGGAAGACTAGCAGTTGAAGGAAGAATTGGTACATACATTCACGATGCACGTATTGGTGTTCTAATCGAAGTGAACTGTGAAACCGACTTTGTTGGCAGAAGTGAGAAATTCAAGGAGTTGGTTGATGATCTTGCAATGCAAGTTGCTGCTTGTCCACAGGTTCAGTTTATATCTATTGAAGATATTATCGAAACTATTGTGACGAAGGAAAAAGAACTTGAGATTCAAAGAGAAGACCTTGCTTCGAAACCCGAGAACATTAGAGAGAAAATTGTCGAGGGAAGAATCTCAAAGAGGTTAGGAGAGCTTGCTCTTCTAGAGATGCCTTTTATTAAGGATGACAGTGTTGTAGTGAAAGACTTGGTGAGGCAAAGTATAGCTCCTATTGGAGAGAACATTAAAGTGCGGCGTTTTCTTCGGTTCACTCTCGGAGAAACAGTTCAGAAAGAAACAGCCGAACTAGCATAG

Protein sequence

>MS.gene061499.t1

MLWCRKSAVVVSAALVKQLREEIGAGMMECKKALAETEGDLEKAQAYLRKKGLSSADKKSGRLAVEGRIGTYIHDARIGVLIEVNCETDFVGRSEKFKELVDDLAMQVAACPQVQFISIEDIIETIVTKEKELEIQREDLASKPENIREKIVEGRISKRLGELALLEMPFIKDDSVVVKDLVRQSIAPIGENIKVRRFLRFTLGETVQKETAELA