Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061920.t1 | XP_003625536.2 | 93.3 | 163 | 11 | 0 | 1 | 163 | 1 | 163 | 9.30E-77 | 296.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061920.t1 | F4J4N3 | 51.8 | 114 | 44 | 2 | 9 | 120 | 8 | 112 | 3.8e-26 | 119.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061920.t1 | G7L0N9 | 93.3 | 163 | 11 | 0 | 1 | 163 | 1 | 163 | 6.7e-77 | 296.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene061920 | -0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene061920 | 0.822857 | 1.89E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene061920 | 0.802059 | 6.71E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene061920 | 0.801443 | 8.98E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene061920 | 0.800675 | 1.29E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061920.t1 | MTR_7g100230 | 92.763 | 152 | 11 | 0 | 12 | 163 | 12 | 163 | 3.09e-92 | 265 |
| MS.gene061920.t1 | MTR_7g100200 | 60.248 | 161 | 61 | 2 | 3 | 163 | 2 | 159 | 5.14e-54 | 168 |
| MS.gene061920.t1 | MTR_7g100170 | 49.057 | 53 | 26 | 1 | 65 | 116 | 75 | 127 | 1.26e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061920.t1 | AT3G58090 | 51.754 | 114 | 44 | 2 | 9 | 120 | 8 | 112 | 2.49e-33 | 119 |
Find 53 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCGAACCACATAGGAATT+TGG | 0.220618 | 7.2:-11818261 | MS.gene061920:CDS |
| AAGAAATCTATTCCTAAATC+TGG | 0.252000 | 7.2:-11818014 | MS.gene061920:CDS |
| GCACTACTCTTCCCAGATTT+AGG | 0.253924 | 7.2:+11818002 | None:intergenic |
| CTGCTACCGGATACTATTTC+AGG | 0.274069 | 7.2:+11818323 | None:intergenic |
| TGGGAGAATGGTTTGTGGGG+AGG | 0.323895 | 7.2:-11818235 | MS.gene061920:CDS |
| AGAGGGCAAAGAAGAAAATA+AGG | 0.333305 | 7.2:-11818417 | MS.gene061920:CDS |
| GGGAGAATGGTTTGTGGGGA+GGG | 0.334434 | 7.2:-11818234 | MS.gene061920:CDS |
| ATCGAACCACATAGGAATTT+GGG | 0.364456 | 7.2:-11818260 | MS.gene061920:CDS |
| GGCTTGGGAGAATGGTTTGT+GGG | 0.370784 | 7.2:-11818239 | MS.gene061920:CDS |
| ACCGAGTATGACAAAGAATC+TGG | 0.379264 | 7.2:-11818167 | MS.gene061920:CDS |
| GGAATTTGGGCTTGGGAGAA+TGG | 0.402637 | 7.2:-11818247 | MS.gene061920:CDS |
| ACCACATAGGAATTTGGGCT+TGG | 0.411883 | 7.2:-11818255 | MS.gene061920:CDS |
| GGGCTTGGGAGAATGGTTTG+TGG | 0.416018 | 7.2:-11818240 | MS.gene061920:CDS |
| CATTGGCGCAGAGGGTTGTT+CGG | 0.423791 | 7.2:-11818043 | MS.gene061920:CDS |
| ATCAAAACAAAGAGAACAAA+AGG | 0.438164 | 7.2:-11817960 | MS.gene061920:CDS |
| GTGATTCTGAAGATCTTGAT+TGG | 0.441563 | 7.2:-11818118 | MS.gene061920:CDS |
| GACGGCGCTGCTGCTGCTAC+CGG | 0.450572 | 7.2:+11818310 | None:intergenic |
| TCTTGATTGGCCGGAGTTGA+AGG | 0.457279 | 7.2:-11818105 | MS.gene061920:CDS |
| GAAGTTCTCAGTCCTTTAGA+TGG | 0.458489 | 7.2:-11818083 | MS.gene061920:CDS |
| ATTACAATCGCAGGCTCTCC+AGG | 0.465049 | 7.2:+11818356 | None:intergenic |
| ATCTAATTCTTCTGCGGCAA+GGG | 0.467917 | 7.2:-11818390 | MS.gene061920:CDS |
| CCCAGATTCTTTGTCATACT+CGG | 0.473382 | 7.2:+11818166 | None:intergenic |
| AGCATTGCTGCTGGAAGGGA+CGG | 0.473470 | 7.2:+11818292 | None:intergenic |
| TTCTGAAGATCTTGATTGGC+CGG | 0.482353 | 7.2:-11818114 | MS.gene061920:CDS |
| GCTTGGGAGAATGGTTTGTG+GGG | 0.486598 | 7.2:-11818238 | MS.gene061920:CDS |
| CCACATAGGAATTTGGGCTT+GGG | 0.489077 | 7.2:-11818254 | MS.gene061920:CDS |
| AGTGAAATTCCACCATCTAA+AGG | 0.490197 | 7.2:+11818071 | None:intergenic |
| AGAAATCTATTCCTAAATCT+GGG | 0.513142 | 7.2:-11818013 | MS.gene061920:CDS |
| GATACTCAAAGCATTGCTGC+TGG | 0.527679 | 7.2:+11818283 | None:intergenic |
| CTCAAAGCATTGCTGCTGGA+AGG | 0.529613 | 7.2:+11818287 | None:intergenic |
| GTTTATGGGACGTTATTACT+CGG | 0.537141 | 7.2:-11818198 | MS.gene061920:CDS |
| ACTCAAGACATTGGCGCAGA+GGG | 0.544285 | 7.2:-11818051 | MS.gene061920:CDS |
| CTGAGAACTTCCTTCAACTC+CGG | 0.550027 | 7.2:+11818095 | None:intergenic |
| TCAAAGCATTGCTGCTGGAA+GGG | 0.554064 | 7.2:+11818288 | None:intergenic |
| GTTTCTGTTTGAAATGGAAG+AGG | 0.555062 | 7.2:-11818435 | None:intergenic |
| TGGAATTTCACTCAAGACAT+TGG | 0.557096 | 7.2:-11818060 | MS.gene061920:CDS |
| GAGCCTGCGATTGTAATTGA+CGG | 0.559057 | 7.2:-11818350 | MS.gene061920:CDS |
| TTTCTGTTTGAAATGGAAGA+GGG | 0.564578 | 7.2:-11818434 | None:intergenic |
| CCCAAGCCCAAATTCCTATG+TGG | 0.565828 | 7.2:+11818254 | None:intergenic |
| GGGCAAAGAAGAAAATAAGG+AGG | 0.566148 | 7.2:-11818414 | MS.gene061920:CDS |
| CACCGTCTTCATACTCAACA+TGG | 0.568627 | 7.2:+11818138 | None:intergenic |
| CACTCAAGACATTGGCGCAG+AGG | 0.573004 | 7.2:-11818052 | MS.gene061920:CDS |
| TTTATGGGACGTTATTACTC+GGG | 0.573677 | 7.2:-11818197 | MS.gene061920:CDS |
| GTTCTCAGTCCTTTAGATGG+TGG | 0.581755 | 7.2:-11818080 | MS.gene061920:CDS |
| GATCTAATTCTTCTGCGGCA+AGG | 0.585721 | 7.2:-11818391 | MS.gene061920:CDS |
| ACTCCGTCAATTACAATCGC+AGG | 0.591670 | 7.2:+11818347 | None:intergenic |
| GGAGTGCCTGAAATAGTATC+CGG | 0.596939 | 7.2:-11818329 | MS.gene061920:CDS |
| CCGAGTATGACAAAGAATCT+GGG | 0.597649 | 7.2:-11818166 | MS.gene061920:CDS |
| TGAGTATCATCGAACCACAT+AGG | 0.617574 | 7.2:-11818268 | MS.gene061920:CDS |
| GGCAAAGAAGAAAATAAGGA+GGG | 0.632888 | 7.2:-11818413 | MS.gene061920:CDS |
| GGAGGGATCTAATTCTTCTG+CGG | 0.660755 | 7.2:-11818396 | MS.gene061920:CDS |
| TACCATGTTGAGTATGAAGA+CGG | 0.707224 | 7.2:-11818140 | MS.gene061920:CDS |
| AGTATGACAAAGAATCTGGG+TGG | 0.768571 | 7.2:-11818163 | MS.gene061920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATGTTCAAAAATTGTTTAT+GGG | - | chr7.2:11818163-11818182 | MS.gene061920:CDS | 20.0% |
| ! | AAGAAATCTATTCCTAAATC+TGG | - | chr7.2:11818361-11818380 | MS.gene061920:CDS | 25.0% |
| ! | AGAAATCTATTCCTAAATCT+GGG | - | chr7.2:11818362-11818381 | MS.gene061920:CDS | 25.0% |
| ! | ATCAAAACAAAGAGAACAAA+AGG | - | chr7.2:11818415-11818434 | MS.gene061920:CDS | 25.0% |
| ! | GGATGTTCAAAAATTGTTTA+TGG | - | chr7.2:11818162-11818181 | MS.gene061920:CDS | 25.0% |
| !!! | GTTCTCTTTGTTTTGATTTG+AGG | + | chr7.2:11818412-11818431 | None:intergenic | 30.0% |
| AGAGGGCAAAGAAGAAAATA+AGG | - | chr7.2:11817958-11817977 | MS.gene061920:CDS | 35.0% | |
| AGTGAAATTCCACCATCTAA+AGG | + | chr7.2:11818307-11818326 | None:intergenic | 35.0% | |
| ATCGAACCACATAGGAATTT+GGG | - | chr7.2:11818115-11818134 | MS.gene061920:CDS | 35.0% | |
| GGCAAAGAAGAAAATAAGGA+GGG | - | chr7.2:11817962-11817981 | MS.gene061920:CDS | 35.0% | |
| GTTTATGGGACGTTATTACT+CGG | - | chr7.2:11818177-11818196 | MS.gene061920:CDS | 35.0% | |
| TACCATGTTGAGTATGAAGA+CGG | - | chr7.2:11818235-11818254 | MS.gene061920:CDS | 35.0% | |
| TGGAATTTCACTCAAGACAT+TGG | - | chr7.2:11818315-11818334 | MS.gene061920:CDS | 35.0% | |
| TTTATGGGACGTTATTACTC+GGG | - | chr7.2:11818178-11818197 | MS.gene061920:CDS | 35.0% | |
| ! | GTGATTCTGAAGATCTTGAT+TGG | - | chr7.2:11818257-11818276 | MS.gene061920:CDS | 35.0% |
| ACCGAGTATGACAAAGAATC+TGG | - | chr7.2:11818208-11818227 | MS.gene061920:CDS | 40.0% | |
| AGTATGACAAAGAATCTGGG+TGG | - | chr7.2:11818212-11818231 | MS.gene061920:CDS | 40.0% | |
| ATCTAATTCTTCTGCGGCAA+GGG | - | chr7.2:11817985-11818004 | MS.gene061920:CDS | 40.0% | |
| CATCGAACCACATAGGAATT+TGG | - | chr7.2:11818114-11818133 | MS.gene061920:CDS | 40.0% | |
| CCCAGATTCTTTGTCATACT+CGG | + | chr7.2:11818212-11818231 | None:intergenic | 40.0% | |
| CCGAGTATGACAAAGAATCT+GGG | - | chr7.2:11818209-11818228 | MS.gene061920:CDS | 40.0% | |
| GAAGTTCTCAGTCCTTTAGA+TGG | - | chr7.2:11818292-11818311 | MS.gene061920:CDS | 40.0% | |
| GGGCAAAGAAGAAAATAAGG+AGG | - | chr7.2:11817961-11817980 | MS.gene061920:CDS | 40.0% | |
| TGAGTATCATCGAACCACAT+AGG | - | chr7.2:11818107-11818126 | MS.gene061920:CDS | 40.0% | |
| ! | GCAAGGGTTTATGATTTTCC+TGG | - | chr7.2:11818001-11818020 | MS.gene061920:CDS | 40.0% |
| ! | TTCTGAAGATCTTGATTGGC+CGG | - | chr7.2:11818261-11818280 | MS.gene061920:CDS | 40.0% |
| ACCACATAGGAATTTGGGCT+TGG | - | chr7.2:11818120-11818139 | MS.gene061920:CDS | 45.0% | |
| ACTCCGTCAATTACAATCGC+AGG | + | chr7.2:11818031-11818050 | None:intergenic | 45.0% | |
| CACCGTCTTCATACTCAACA+TGG | + | chr7.2:11818240-11818259 | None:intergenic | 45.0% | |
| CCACATAGGAATTTGGGCTT+GGG | - | chr7.2:11818121-11818140 | MS.gene061920:CDS | 45.0% | |
| CTGAGAACTTCCTTCAACTC+CGG | + | chr7.2:11818283-11818302 | None:intergenic | 45.0% | |
| CTGCTACCGGATACTATTTC+AGG | + | chr7.2:11818055-11818074 | None:intergenic | 45.0% | |
| GAGCCTGCGATTGTAATTGA+CGG | - | chr7.2:11818025-11818044 | MS.gene061920:CDS | 45.0% | |
| GATCTAATTCTTCTGCGGCA+AGG | - | chr7.2:11817984-11818003 | MS.gene061920:CDS | 45.0% | |
| GCACTACTCTTCCCAGATTT+AGG | + | chr7.2:11818376-11818395 | None:intergenic | 45.0% | |
| GGAGGGATCTAATTCTTCTG+CGG | - | chr7.2:11817979-11817998 | MS.gene061920:CDS | 45.0% | |
| GTTCTCAGTCCTTTAGATGG+TGG | - | chr7.2:11818295-11818314 | MS.gene061920:CDS | 45.0% | |
| ! | GGAGTGCCTGAAATAGTATC+CGG | - | chr7.2:11818046-11818065 | MS.gene061920:CDS | 45.0% |
| !! | GATACTCAAAGCATTGCTGC+TGG | + | chr7.2:11818095-11818114 | None:intergenic | 45.0% |
| !! | TCAAAGCATTGCTGCTGGAA+GGG | + | chr7.2:11818090-11818109 | None:intergenic | 45.0% |
| ACTCAAGACATTGGCGCAGA+GGG | - | chr7.2:11818324-11818343 | MS.gene061920:CDS | 50.0% | |
| ATTACAATCGCAGGCTCTCC+AGG | + | chr7.2:11818022-11818041 | None:intergenic | 50.0% | |
| CCCAAGCCCAAATTCCTATG+TGG | + | chr7.2:11818124-11818143 | None:intergenic | 50.0% | |
| GGAATTTGGGCTTGGGAGAA+TGG | - | chr7.2:11818128-11818147 | MS.gene061920:CDS | 50.0% | |
| ! | GCTTGGGAGAATGGTTTGTG+GGG | - | chr7.2:11818137-11818156 | MS.gene061920:CDS | 50.0% |
| ! | GGCTTGGGAGAATGGTTTGT+GGG | - | chr7.2:11818136-11818155 | MS.gene061920:CDS | 50.0% |
| ! | TCTTGATTGGCCGGAGTTGA+AGG | - | chr7.2:11818270-11818289 | MS.gene061920:CDS | 50.0% |
| !! | CTCAAAGCATTGCTGCTGGA+AGG | + | chr7.2:11818091-11818110 | None:intergenic | 50.0% |
| AGCATTGCTGCTGGAAGGGA+CGG | + | chr7.2:11818086-11818105 | None:intergenic | 55.0% | |
| CACTCAAGACATTGGCGCAG+AGG | - | chr7.2:11818323-11818342 | MS.gene061920:CDS | 55.0% | |
| CATTGGCGCAGAGGGTTGTT+CGG | - | chr7.2:11818332-11818351 | MS.gene061920:CDS | 55.0% | |
| GGGCTTGGGAGAATGGTTTG+TGG | - | chr7.2:11818135-11818154 | MS.gene061920:CDS | 55.0% | |
| ! | GGGAGAATGGTTTGTGGGGA+GGG | - | chr7.2:11818141-11818160 | MS.gene061920:CDS | 55.0% |
| ! | TGGGAGAATGGTTTGTGGGG+AGG | - | chr7.2:11818140-11818159 | MS.gene061920:CDS | 55.0% |
| GACGGCGCTGCTGCTGCTAC+CGG | + | chr7.2:11818068-11818087 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 11817953 | 11818444 | 11817953 | ID=MS.gene061920 |
| chr7.2 | mRNA | 11817953 | 11818444 | 11817953 | ID=MS.gene061920.t1;Parent=MS.gene061920 |
| chr7.2 | exon | 11817953 | 11818444 | 11817953 | ID=MS.gene061920.t1.exon1;Parent=MS.gene061920.t1 |
| chr7.2 | CDS | 11817953 | 11818444 | 11817953 | ID=cds.MS.gene061920.t1;Parent=MS.gene061920.t1 |
| Gene Sequence |
| Protein sequence |
