Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062416.t1 | RHN44803.1 | 95.6 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 4.80E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062416.t1 | A0A072TYJ6 | 95.6 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.4e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052606 | MS.gene062416 | 0.808439 | 3.10E-50 | -1.69E-46 |
| MS.gene053097 | MS.gene062416 | 0.831005 | 2.13E-55 | -1.69E-46 |
| MS.gene055158 | MS.gene062416 | 0.804089 | 2.55E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene062416 | 0.805756 | 1.14E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene062416 | 0.824579 | 7.48E-54 | -1.69E-46 |
| MS.gene058173 | MS.gene062416 | 0.82613 | 3.21E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene062416 | 0.827034 | 1.96E-54 | -1.69E-46 |
| MS.gene059545 | MS.gene062416 | 0.828872 | 7.06E-55 | -1.69E-46 |
| MS.gene05960 | MS.gene062416 | 0.810124 | 1.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062416.t1 | MTR_7g024873 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 4.35e-94 | 268 |
| MS.gene062416.t1 | MTR_7g024880 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.76e-92 | 263 |
| MS.gene062416.t1 | MTR_7g024890 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 2.42e-92 | 263 |
| MS.gene062416.t1 | MTR_8g446810 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 1.10e-40 | 132 |
| MS.gene062416.t1 | MTR_7g024900 | 96.078 | 51 | 2 | 0 | 1 | 51 | 1 | 51 | 8.90e-29 | 100 |
| MS.gene062416.t1 | MTR_1g081270 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 6.89e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062416.t1 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.27e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7.3:+75145823 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7.3:+75146078 | MS.gene062416:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7.3:-75146025 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7.3:-75145868 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7.3:+75146159 | MS.gene062416:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7.3:-75146163 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7.3:+75145881 | MS.gene062416:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7.3:+75145888 | MS.gene062416:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7.3:-75145862 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7.3:+75146153 | MS.gene062416:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7.3:-75146024 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7.3:+75145964 | MS.gene062416:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7.3:+75146064 | MS.gene062416:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7.3:+75145946 | MS.gene062416:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7.3:+75146132 | MS.gene062416:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7.3:+75145963 | MS.gene062416:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7.3:+75145954 | MS.gene062416:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7.3:+75146210 | MS.gene062416:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7.3:+75146133 | MS.gene062416:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7.3:+75146127 | MS.gene062416:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7.3:+75145994 | MS.gene062416:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7.3:-75145852 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7.3:+75145917 | MS.gene062416:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7.3:+75146120 | MS.gene062416:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7.3:-75145919 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7.3:+75146128 | MS.gene062416:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7.3:75145871-75145890 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | + | chr7.3:75145888-75145907 | MS.gene062416:CDS | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7.3:75145827-75145846 | MS.gene062416:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7.3:75146210-75146229 | MS.gene062416:CDS | 30.0% |
| CTCTTATGATGAGATGTTGA+AGG | + | chr7.3:75145946-75145965 | MS.gene062416:CDS | 35.0% | |
| CTTGAATGGCACAATTGATT+TGG | + | chr7.3:75146078-75146097 | MS.gene062416:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7.3:75146166-75146185 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | + | chr7.3:75145881-75145900 | MS.gene062416:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7.3:75145994-75146013 | MS.gene062416:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7.3:75146198-75146217 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7.3:75145983-75146002 | MS.gene062416:CDS | 35.0% |
| AAAGCAGATGATGCTTGCAA+TGG | + | chr7.3:75146120-75146139 | MS.gene062416:CDS | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7.3:75146064-75146083 | MS.gene062416:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7.3:75146159-75146178 | MS.gene062416:CDS | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7.3:75145865-75145884 | None:intergenic | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7.3:75146153-75146172 | MS.gene062416:CDS | 40.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7.3:75145855-75145874 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | - | chr7.3:75145976-75145995 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7.3:75146027-75146046 | None:intergenic | 45.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | + | chr7.3:75145954-75145973 | MS.gene062416:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7.3:75146127-75146146 | MS.gene062416:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7.3:75146028-75146047 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7.3:75146128-75146147 | MS.gene062416:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7.3:75146133-75146152 | MS.gene062416:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | - | chr7.3:75145922-75145941 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7.3:75146132-75146151 | MS.gene062416:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | + | chr7.3:75145917-75145936 | MS.gene062416:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7.3:75145964-75145983 | MS.gene062416:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7.3:75145963-75145982 | MS.gene062416:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75145827 | 75146240 | 75145827 | ID=MS.gene062416 |
| chr7.3 | mRNA | 75145827 | 75146240 | 75145827 | ID=MS.gene062416.t1;Parent=MS.gene062416 |
| chr7.3 | exon | 75145827 | 75146240 | 75145827 | ID=MS.gene062416.t1.exon1;Parent=MS.gene062416.t1 |
| chr7.3 | CDS | 75145827 | 75146240 | 75145827 | ID=cds.MS.gene062416.t1;Parent=MS.gene062416.t1 |
| Gene Sequence |
| Protein sequence |
