AlfalfaGEDB | MS.gene06255

M. sativa cultivar XinJiangDaYe / MS.gene06255

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06255.t1	XP_013461663.1	96	303	10	2	1	302	1	302	2.60E-125	458.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06255.t1	Q93W88	56.2	162	55	1	138	299	127	272	1.4e-39	164.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06255.t1	A0A072V2W2	96.0	303	10	2	1	302	1	302	1.9e-125	458.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene06255.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054955	MS.gene06255	0.826327	2.88E-54	-1.69E-46
MS.gene055533	MS.gene06255	0.981223	4.54E-152	-1.69E-46
MS.gene057146	MS.gene06255	0.81522	1.03E-51	-1.69E-46
MS.gene057927	MS.gene06255	0.8107	1.01E-50	-1.69E-46
MS.gene058660	MS.gene06255	0.806438	8.23E-50	-1.69E-46
MS.gene060804	MS.gene06255	0.806419	8.30E-50	-1.69E-46
MS.gene060818	MS.gene06255	0.800661	1.30E-48	-1.69E-46
MS.gene061041	MS.gene06255	0.837578	4.79E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06255.t1	MTR_3g498825	98.350	303	3	2	1	302	1	302	0.0	607
MS.gene06255.t1	MTR_8g099880	59.032	310	94	11	1	295	1	292	2.26e-95	285
MS.gene06255.t1	MTR_1g017350	60.000	125	41	2	121	239	228	349	2.19e-43	154
MS.gene06255.t1	MTR_1g017350	60.000	125	41	2	121	239	228	349	3.10e-43	153
MS.gene06255.t1	MTR_3g116770	63.200	125	30	1	111	219	139	263	7.05e-43	152
MS.gene06255.t1	MTR_3g116770	63.200	125	30	1	111	219	139	263	7.51e-43	152
MS.gene06255.t1	MTR_7g053410	57.516	153	45	3	86	218	236	388	1.27e-42	153
MS.gene06255.t1	MTR_1g017350	61.667	120	37	2	121	234	228	344	1.55e-42	151
MS.gene06255.t1	MTR_8g065740	69.091	110	31	1	113	219	268	377	1.87e-42	152
MS.gene06255.t1	MTR_8g012290	62.500	136	40	4	109	236	245	377	2.18e-42	152
MS.gene06255.t1	MTR_8g012290	62.500	136	40	4	109	236	245	377	2.26e-42	152
MS.gene06255.t1	MTR_7g053410	57.516	153	45	3	86	218	236	388	2.29e-42	153
MS.gene06255.t1	MTR_1g017350	61.667	120	37	2	121	234	228	344	2.36e-42	151
MS.gene06255.t1	MTR_5g037250	61.475	122	45	2	98	218	63	183	3.16e-42	145
MS.gene06255.t1	MTR_5g048860	56.738	141	49	2	91	219	297	437	4.21e-41	150
MS.gene06255.t1	MTR_4g070320	70.642	109	28	3	113	219	109	215	2.32e-40	144
MS.gene06255.t1	MTR_8g062240	86.667	75	10	0	144	218	148	222	8.05e-40	140
MS.gene06255.t1	MTR_1g059270	55.652	115	42	2	144	252	157	268	2.23e-36	133
MS.gene06255.t1	MTR_1g059270	65.217	92	29	1	144	235	157	245	2.84e-36	130
MS.gene06255.t1	MTR_7g092510	68.889	90	25	1	144	233	170	256	2.15e-35	130
MS.gene06255.t1	MTR_6g084120	50.000	124	33	5	112	231	134	232	6.10e-27	106
MS.gene06255.t1	MTR_4g131160	41.722	151	71	4	147	293	196	333	1.86e-23	99.4
MS.gene06255.t1	MTR_4g131160	41.722	151	71	4	147	293	199	336	2.31e-23	99.4
MS.gene06255.t1	MTR_1g080890	61.039	77	29	1	136	212	182	257	1.56e-21	93.6
MS.gene06255.t1	MTR_4g033295	60.000	65	26	0	147	211	118	182	6.35e-18	80.5
MS.gene06255.t1	MTR_4g033295	58.209	67	27	1	147	212	118	184	9.08e-18	79.7
MS.gene06255.t1	MTR_4g065870	61.538	65	25	0	147	211	355	419	5.56e-17	81.3
MS.gene06255.t1	MTR_4g065870	61.290	62	24	0	147	208	355	416	2.79e-16	79.0
MS.gene06255.t1	MTR_1g069155	40.952	105	60	2	118	221	287	390	4.22e-16	79.0
MS.gene06255.t1	MTR_1g052470	50.633	79	39	0	147	225	298	376	6.61e-16	77.8
MS.gene06255.t1	MTR_2g098310	56.923	65	28	0	147	211	332	396	1.41e-15	77.0
MS.gene06255.t1	MTR_1g070870	40.909	132	72	2	110	236	78	208	2.06e-15	75.1
MS.gene06255.t1	MTR_7g092540	53.731	67	31	0	147	213	243	309	4.53e-15	74.7
MS.gene06255.t1	MTR_2g098310	56.061	66	29	0	145	210	330	395	4.64e-15	75.5
MS.gene06255.t1	MTR_1g070870	48.980	98	49	1	139	236	115	211	6.34e-15	73.9
MS.gene06255.t1	MTR_1g084980	37.273	110	64	3	107	212	418	526	8.13e-15	75.1
MS.gene06255.t1	MTR_7g092540	54.688	64	29	0	147	210	243	306	8.62e-15	73.9
MS.gene06255.t1	MTR_3g028520	53.846	65	30	0	147	211	322	386	4.01e-14	72.8
MS.gene06255.t1	MTR_3g027650	53.846	65	30	0	147	211	322	386	4.01e-14	72.8
MS.gene06255.t1	MTR_3g027650	54.839	62	28	0	147	208	322	383	1.73e-13	70.9
MS.gene06255.t1	MTR_7g099540	48.611	72	36	1	150	221	255	325	2.77e-13	70.1
MS.gene06255.t1	MTR_5g017040	37.391	115	67	3	112	222	67	180	1.48e-12	67.8
MS.gene06255.t1	MTR_3g449770	47.945	73	36	2	149	221	311	381	2.34e-12	67.4
MS.gene06255.t1	MTR_8g036235	49.231	65	33	0	147	211	316	380	2.52e-12	67.0
MS.gene06255.t1	MTR_1g019240	39.130	92	54	2	149	239	87	177	6.18e-12	65.1
MS.gene06255.t1	MTR_1g018090	53.125	64	29	1	148	211	275	337	8.60e-12	65.5
MS.gene06255.t1	MTR_4g088710	96.552	29	1	0	275	303	9	37	1.32e-11	59.7
MS.gene06255.t1	MTR_1g116790	50.000	64	32	0	147	210	265	328	1.69e-11	64.3
MS.gene06255.t1	MTR_2g098310	43.750	96	46	3	147	235	332	426	3.58e-11	63.9
MS.gene06255.t1	MTR_7g099540	44.872	78	36	2	150	221	255	331	4.56e-11	63.5
MS.gene06255.t1	MTR_3g103030	48.052	77	38	2	148	223	230	305	5.12e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06255.t1	AT5G50915	48.069	233	97	6	74	299	57	272	3.32e-59	191
MS.gene06255.t1	AT5G50915	48.069	233	97	6	74	299	57	272	3.32e-59	191
MS.gene06255.t1	AT5G50915	50.228	219	82	6	74	286	57	254	4.17e-59	190
MS.gene06255.t1	AT5G50915	50.228	219	82	6	74	286	57	254	4.17e-59	190
MS.gene06255.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.26e-46	164
MS.gene06255.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.26e-46	164
MS.gene06255.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.26e-46	164
MS.gene06255.t1	AT1G68920	56.209	153	54	2	92	234	237	386	1.36e-46	163
MS.gene06255.t1	AT1G59640	68.966	116	31	2	108	218	92	207	9.32e-45	153
MS.gene06255.t1	AT1G10120	60.976	123	45	1	112	234	171	290	1.01e-44	156
MS.gene06255.t1	AT1G10120	60.976	123	45	1	112	234	171	290	1.01e-44	156
MS.gene06255.t1	AT3G07340	65.079	126	43	1	93	218	205	329	2.62e-44	156
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	90	256	3.47e-44	154
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	119	285	4.44e-44	154
MS.gene06255.t1	AT3G07340	65.079	126	43	1	93	218	205	329	4.84e-44	156
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	141	307	6.70e-44	154
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	141	307	6.70e-44	154
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	141	307	6.70e-44	154
MS.gene06255.t1	AT1G26260	52.353	170	49	4	94	234	141	307	6.70e-44	154
MS.gene06255.t1	AT1G59640	68.966	116	31	2	108	218	92	207	1.62e-43	152
MS.gene06255.t1	AT5G48560	83.146	89	15	0	139	227	293	381	2.00e-43	155
MS.gene06255.t1	AT5G48560	83.146	89	15	0	139	227	293	381	3.68e-43	154
MS.gene06255.t1	AT3G23690	60.000	135	41	4	112	234	146	279	5.80e-42	149
MS.gene06255.t1	AT4G34530	87.179	78	10	0	142	219	167	244	3.29e-41	146
MS.gene06255.t1	AT5G62610	71.910	89	25	0	130	218	136	224	7.48e-39	138
MS.gene06255.t1	AT5G62610	71.910	89	25	0	130	218	136	224	7.48e-39	138
MS.gene06255.t1	AT4G36540	57.143	133	46	3	111	235	97	226	2.61e-38	137
MS.gene06255.t1	AT4G36540	57.143	133	46	3	111	235	97	226	2.82e-38	137
MS.gene06255.t1	AT2G18300	49.057	159	70	4	85	243	131	278	7.29e-38	137
MS.gene06255.t1	AT2G18300	55.556	135	52	3	85	219	131	257	1.50e-37	136
MS.gene06255.t1	AT2G18300	55.556	135	52	3	85	219	131	257	2.49e-37	136
MS.gene06255.t1	AT2G42300	64.583	96	31	1	144	239	182	274	6.84e-37	133
MS.gene06255.t1	AT1G18400	71.264	87	24	1	145	231	143	228	7.68e-37	132
MS.gene06255.t1	AT3G57800	71.111	90	23	1	144	233	201	287	9.49e-37	135
MS.gene06255.t1	AT1G18400	71.264	87	24	1	145	231	143	228	1.12e-36	132
MS.gene06255.t1	AT1G73830	68.478	92	28	1	146	237	146	236	4.43e-36	130
MS.gene06255.t1	AT1G73830	70.930	86	24	1	146	231	146	230	9.22e-36	129
MS.gene06255.t1	AT2G42300	66.304	92	28	1	144	235	182	270	1.26e-35	131
MS.gene06255.t1	AT1G25330	52.518	139	54	4	99	231	51	183	9.85e-34	123
MS.gene06255.t1	AT3G57800	46.763	139	24	2	144	235	201	336	5.90e-28	112
MS.gene06255.t1	AT2G24260	65.753	73	24	1	140	212	28	99	1.41e-23	97.1
MS.gene06255.t1	AT2G24260	65.753	73	24	1	140	212	131	202	1.17e-22	96.7
MS.gene06255.t1	AT5G58010	52.941	102	37	3	147	244	99	193	2.34e-22	95.1
MS.gene06255.t1	AT4G30980	71.212	66	18	1	147	212	130	194	3.32e-22	94.7
MS.gene06255.t1	AT4G30980	71.212	66	18	1	147	212	178	242	4.67e-22	95.1
MS.gene06255.t1	AT2G42300	83.721	43	7	0	144	186	182	224	6.18e-19	84.0
MS.gene06255.t1	AT2G42300	83.721	43	7	0	144	186	182	224	6.18e-19	84.0
MS.gene06255.t1	AT2G42300	83.721	43	7	0	144	186	182	224	7.63e-19	84.0
MS.gene06255.t1	AT4G09180	53.731	67	31	0	145	211	182	248	3.41e-15	74.3
MS.gene06255.t1	AT1G35460	53.846	65	30	0	147	211	181	245	3.48e-15	74.3
MS.gene06255.t1	AT2G42280	54.688	64	29	0	147	210	279	342	5.59e-15	75.1
MS.gene06255.t1	AT2G42280	56.061	66	27	1	147	210	279	344	8.45e-15	74.3
MS.gene06255.t1	AT1G51140	57.812	64	27	0	147	210	304	367	8.82e-15	74.3
MS.gene06255.t1	AT4G28790	47.297	74	38	1	149	222	273	345	1.71e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene06255.t1	AT4G02590	40.310	129	63	4	109	224	36	163	4.54e-14	70.9
MS.gene06255.t1	AT4G28800	48.611	72	36	1	150	221	252	322	4.83e-14	72.4
MS.gene06255.t1	AT4G02590	40.310	129	63	4	109	224	99	226	5.06e-14	71.6
MS.gene06255.t1	AT4G02590	40.310	129	63	4	109	224	99	226	5.06e-14	71.6
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.65e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.65e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.65e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	281	342	6.95e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	281	342	6.95e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	281	342	6.95e-14	72.0
MS.gene06255.t1	AT2G20180	52.381	63	29	1	150	212	281	342	6.95e-14	72.0
MS.gene06255.t1	AT4G28815	50.000	70	33	2	150	218	71	139	7.34e-14	70.1
MS.gene06255.t1	AT4G28815	50.000	70	33	2	150	218	147	215	2.45e-13	69.7
MS.gene06255.t1	AT1G03040	53.659	82	33	2	147	224	139	219	3.96e-13	68.9
MS.gene06255.t1	AT1G03040	53.659	82	33	2	147	224	144	224	4.37e-13	68.9
MS.gene06255.t1	AT2G43140	53.125	64	30	0	147	210	134	197	6.08e-13	67.0
MS.gene06255.t1	AT2G43140	53.125	64	30	0	147	210	231	294	7.13e-13	68.2
MS.gene06255.t1	AT1G03040	53.659	82	33	2	147	224	144	224	7.76e-13	68.6
MS.gene06255.t1	AT4G28800	52.459	61	28	1	150	210	264	323	1.37e-12	68.2
MS.gene06255.t1	AT2G20180	51.667	60	28	1	150	209	281	339	1.86e-12	67.4
MS.gene06255.t1	AT1G27740	36.170	141	72	5	86	223	118	243	3.17e-12	65.9
MS.gene06255.t1	AT4G00050	49.275	69	34	1	143	211	101	168	1.26e-11	64.7
MS.gene06255.t1	AT4G00050	49.275	69	34	1	143	211	203	270	2.27e-11	64.3
MS.gene06255.t1	AT1G05805	48.438	64	33	0	147	210	283	346	2.28e-11	64.3
MS.gene06255.t1	AT4G00050	49.275	69	34	1	143	211	203	270	2.81e-11	63.9
MS.gene06255.t1	AT4G28790	45.455	66	35	1	149	214	273	337	4.54e-11	63.2
MS.gene06255.t1	AT2G14760	45.349	86	42	3	147	228	237	321	4.97e-11	63.2
MS.gene06255.t1	AT4G33880	50.769	65	31	1	147	211	266	329	9.71e-11	62.4

Find 63 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAGGGTTAGTATTGTTTC+TGG	0.191984	3.1:-78634035	None:intergenic
TCCACAGTCTTCCATGGTTC+TGG	0.296713	3.1:+78633946	MS.gene06255:CDS
GCAAAAGTTGTGCCTTGTTT+AGG	0.309108	3.1:-78635291	None:intergenic
CTCAGTTATGGTAGTAACTT+TGG	0.320242	3.1:-78633891	None:intergenic
AGCTGCTAATGACTACCTTT+TGG	0.330118	3.1:+78635350	MS.gene06255:CDS
ATGGATTGTAACCAGGTAAC+TGG	0.332974	3.1:+78634874	MS.gene06255:intron
AAAAGATCAGAGAAAGTGTT+TGG	0.372452	3.1:+78634446	MS.gene06255:CDS
GGACGTGGATATAGCCAGTT+GGG	0.373564	3.1:-78634476	None:intergenic
AACTGTGAAGGTGGAGGAGG+AGG	0.387298	3.1:-78633821	None:intergenic
AACAGACCTAGACACTCTTT+TGG	0.397586	3.1:+78635105	MS.gene06255:CDS
GTTCTGGACAAGCTTGAAAA+TGG	0.416319	3.1:+78633962	MS.gene06255:CDS
TGAAACTGTGAAGGTGGAGG+AGG	0.419275	3.1:-78633824	None:intergenic
AGAAGGTTCATTCTCAGTTA+TGG	0.434337	3.1:-78633903	None:intergenic
TTATCAGAAATTAAATAACT+TGG	0.449559	3.1:+78635227	MS.gene06255:intron
TGGCCTGACCCCTTCTTGCT+CGG	0.454614	3.1:-78634497	None:intergenic
TAGCCACAGCCTTGCTGAAA+GGG	0.456249	3.1:+78634524	MS.gene06255:CDS
GACCCCTTCTTGCTCGGACG+TGG	0.465774	3.1:-78634491	None:intergenic
CGTCCGAGCAAGAAGGGGTC+AGG	0.472206	3.1:+78634494	MS.gene06255:CDS
GAAGATGTTGGTTGGAGTCA+TGG	0.483883	3.1:-78635325	None:intergenic
GAAGAGAGAAAATAAGTGAA+AGG	0.496748	3.1:+78634687	MS.gene06255:CDS
GACACAGATGATAATGTTGA+TGG	0.508396	3.1:-78635255	None:intergenic
ATTAGCAGCTTGGAAGATGT+TGG	0.513901	3.1:-78635337	None:intergenic
AATATTATTACATGAGAGTG+AGG	0.517794	3.1:-78634052	None:intergenic
TGACAGGATAATGCAGCAGA+AGG	0.520312	3.1:+78634369	MS.gene06255:intron
AAGTTGCAGCAACTTGTGCC+AGG	0.526665	3.1:+78634716	MS.gene06255:CDS
GGGTGAAACTGTGAAGGTGG+AGG	0.526667	3.1:-78633827	None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG	0.530497	3.1:+78634523	MS.gene06255:CDS
ACTTGTGCCAGGCTGTGATA+AGG	0.531492	3.1:+78634727	MS.gene06255:CDS
AAAGGTAGTCATTAGCAGCT+TGG	0.531755	3.1:-78635347	None:intergenic
CCATGGAAGACTGTGGACTA+AGG	0.533292	3.1:-78633940	None:intergenic
CCTTAGTCCACAGTCTTCCA+TGG	0.535388	3.1:+78633940	MS.gene06255:CDS
GCAGCTTGGAAGATGTTGGT+TGG	0.538644	3.1:-78635333	None:intergenic
TTAATAATATCTTGATGAAG+AGG	0.545994	3.1:-78633848	None:intergenic
AGCAAGGCTGTGGCTATCAG+TGG	0.554257	3.1:-78634517	None:intergenic
TATCCACGTCCGAGCAAGAA+GGG	0.556525	3.1:+78634488	MS.gene06255:CDS
TTGAAGCTGAAGTATCCAAA+AGG	0.556829	3.1:-78635365	None:intergenic
GTTCTCCTCTTCTTCTCCGT+AGG	0.559768	3.1:-78634013	None:intergenic
CGGACGTGGATATAGCCAGT+TGG	0.559837	3.1:-78634477	None:intergenic
ATAGTCCTACGGAGAAGAAG+AGG	0.562653	3.1:+78634008	MS.gene06255:CDS
AATGATGAGTCAACAAGGAA+AGG	0.563177	3.1:-78633773	None:intergenic
ATGAGTCAACAAGGAAAGGA+TGG	0.563812	3.1:-78633769	None:intergenic
ATACCCTTTCAGCAAGGCTG+TGG	0.567250	3.1:-78634527	None:intergenic
TGACCTACCTTATCACAGCC+TGG	0.577941	3.1:-78634734	None:intergenic
GTCTAACCAAAAGAGTGTCT+AGG	0.588703	3.1:-78635111	None:intergenic
TTCAAGCTTGTCCAGAACCA+TGG	0.592122	3.1:-78633957	None:intergenic
GTTGCCATGTCATAGAACAT+AGG	0.595363	3.1:-78635085	None:intergenic
ATATCCACGTCCGAGCAAGA+AGG	0.602822	3.1:+78634487	MS.gene06255:CDS
AGAGGGTGAAACTGTGAAGG+TGG	0.604355	3.1:-78633830	None:intergenic
GTCTTTACAGAATCAAGTAG+AGG	0.604540	3.1:+78634936	MS.gene06255:CDS
GTCATCAAATGTTGTCTCTG+AGG	0.606377	3.1:+78635407	MS.gene06255:CDS
TTGTAACCAGGTAACTGGAA+AGG	0.609989	3.1:+78634879	MS.gene06255:intron
ATATTATTACATGAGAGTGA+GGG	0.611838	3.1:-78634051	None:intergenic
TAATAATATCTTGATGAAGA+GGG	0.621927	3.1:-78633847	None:intergenic
GACGTGGATATAGCCAGTTG+GGG	0.624086	3.1:-78634475	None:intergenic
GACAGGATAATGCAGCAGAA+GGG	0.624145	3.1:+78634370	MS.gene06255:intron
ATCCACGTCCGAGCAAGAAG+GGG	0.635705	3.1:+78634489	MS.gene06255:CDS
TCCAGAACCATGGAAGACTG+TGG	0.648687	3.1:-78633947	None:intergenic
GCATGAATGATGAGTCAACA+AGG	0.650211	3.1:-78633778	None:intergenic
CATTGCAATAGTCCTAAACA+AGG	0.657273	3.1:+78635279	MS.gene06255:CDS
TGTACTATACCCTTTCAGCA+AGG	0.661128	3.1:-78634533	None:intergenic
TGAAGAGGGTGAAACTGTGA+AGG	0.663457	3.1:-78633833	None:intergenic
GAATCCTATGTTCTATGACA+TGG	0.666100	3.1:+78635081	MS.gene06255:CDS
ACGTGGATATAGCCAGTTGG+GGG	0.714907	3.1:-78634474	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAATTTACTTAATAATACT+TGG	+	chr3.1:78635681-78635700	MS.gene06255:intron	10.0%
!!	AAAATGATTTAATCCTAAAA+TGG	+	chr3.1:78635452-78635471	MS.gene06255:intron	15.0%
!!	AATTATATAATATAAGAGAC+AGG	-	chr3.1:78635662-78635681	None:intergenic	15.0%
!!	TTATCAGAAATTAAATAACT+TGG	+	chr3.1:78635227-78635246	MS.gene06255:intron	15.0%
!!!	CTTATAATTTTTTTTTCTTC+AGG	+	chr3.1:78635719-78635738	MS.gene06255:intron	15.0%
!!!	GTGAATTTTAAATTAATGAA+AGG	-	chr3.1:78635590-78635609	None:intergenic	15.0%
!!!	TTAGGATTAAATCATTTTTT+TGG	-	chr3.1:78635450-78635469	None:intergenic	15.0%
!!	AAATTGGATGAATTATATTC+AGG	+	chr3.1:78634661-78634680	MS.gene06255:intron	20.0%
!!	TAATAATATCTTGATGAAGA+GGG	-	chr3.1:78633850-78633869	None:intergenic	20.0%
!!	TGTTAGTAATTAAATATGAC+AGG	+	chr3.1:78634353-78634372	MS.gene06255:intron	20.0%
!!	TGTTGCAAATTGAATTAAAT+TGG	+	chr3.1:78634645-78634664	MS.gene06255:intron	20.0%
!!	TTAATAATATCTTGATGAAG+AGG	-	chr3.1:78633851-78633870	None:intergenic	20.0%
!!!	AAAAAATAACATTTTCAGGA+CGG	+	chr3.1:78635502-78635521	MS.gene06255:intron	20.0%
!!!	AAACTTGTGGATAATTTTTT+TGG	+	chr3.1:78634214-78634233	MS.gene06255:intron	20.0%
!!!	AGAATAATTACATCCATTTT+AGG	-	chr3.1:78635468-78635487	None:intergenic	20.0%
!!!	TGACAAAAAATAACATTTTC+AGG	+	chr3.1:78635498-78635517	MS.gene06255:intron	20.0%
!!!	TTAGCACTTTTTTTATACTA+TGG	+	chr3.1:78634247-78634266	MS.gene06255:intron	20.0%
!	AATATTATTACATGAGAGTG+AGG	-	chr3.1:78634055-78634074	None:intergenic	25.0%
!	AGACATGAAGATAACTAAAA+GGG	-	chr3.1:78634616-78634635	None:intergenic	25.0%
!	ATATTATTACATGAGAGTGA+GGG	-	chr3.1:78634054-78634073	None:intergenic	25.0%
!	TAATCAACTCAATTCATAAC+AGG	-	chr3.1:78634319-78634338	None:intergenic	25.0%
!	TATATGATGATTCACATGTT+AGG	+	chr3.1:78635160-78635179	MS.gene06255:intron	25.0%
!!	ACTAGTATTTTCATTTCATG+TGG	+	chr3.1:78634558-78634577	MS.gene06255:intron	25.0%
!!	ATAACTAAAAGGGACATTTT+TGG	-	chr3.1:78634606-78634625	None:intergenic	25.0%
!!	ATTATTTATGGATTGTAACC+AGG	+	chr3.1:78634867-78634886	MS.gene06255:intron	25.0%
!!	CCACAAGTTTTCTTAAAATA+AGG	-	chr3.1:78634204-78634223	None:intergenic	25.0%
!!	CTAGTATTTTCATTTCATGT+GGG	+	chr3.1:78634559-78634578	MS.gene06255:intron	25.0%
!!	TAGTATTTTCATTTCATGTG+GGG	+	chr3.1:78634560-78634579	MS.gene06255:intron	25.0%
!!!	CCTTATTTTAAGAAAACTTG+TGG	+	chr3.1:78634201-78634220	MS.gene06255:intron	25.0%
!!!	TTTGAGACTTTTAATTGCAT+GGG	-	chr3.1:78634789-78634808	None:intergenic	25.0%
!!!	TTTTGAGACTTTTAATTGCA+TGG	-	chr3.1:78634790-78634809	None:intergenic	25.0%
	ATCTCTAATTCTTCTGTCAT+AGG	-	chr3.1:78634167-78634186	None:intergenic	30.0%
	CAAAAGTTTCTTCATTCACA+TGG	+	chr3.1:78635544-78635563	MS.gene06255:intron	30.0%
	GAAAAACAAAGAAGATGAGA+AGG	+	chr3.1:78634410-78634429	MS.gene06255:CDS	30.0%
	GAAGAGAGAAAATAAGTGAA+AGG	+	chr3.1:78634687-78634706	MS.gene06255:CDS	30.0%
	GAGACATGAAGATAACTAAA+AGG	-	chr3.1:78634617-78634636	None:intergenic	30.0%
	TACTCAGAAAAATAGTCCTA+CGG	+	chr3.1:78633997-78634016	MS.gene06255:CDS	30.0%
	TCTAGCAGTGATATATATGA+TGG	-	chr3.1:78634098-78634117	None:intergenic	30.0%
	TTGGAATTGTATCATACAAG+TGG	-	chr3.1:78634587-78634606	None:intergenic	30.0%
!	AGTGATATATATGATGGAGA+TGG	-	chr3.1:78634092-78634111	None:intergenic	30.0%
!	CAGCTTCAATTTTTCTACAA+GGG	+	chr3.1:78635379-78635398	MS.gene06255:CDS	30.0%
!	TCAGCTTCAATTTTTCTACA+AGG	+	chr3.1:78635378-78635397	MS.gene06255:CDS	30.0%
!!	AAAAGATCAGAGAAAGTGTT+TGG	+	chr3.1:78634446-78634465	MS.gene06255:CDS	30.0%
!!	AATATTCTCAAGCTGTTTTG+TGG	+	chr3.1:78634966-78634985	MS.gene06255:intron	30.0%
!!!	AAACATGTTGGTAGTTTTGT+AGG	-	chr3.1:78635625-78635644	None:intergenic	30.0%
!!!	AACATGTTGGTAGTTTTGTA+GGG	-	chr3.1:78635624-78635643	None:intergenic	30.0%
	AATGATGAGTCAACAAGGAA+AGG	-	chr3.1:78633776-78633795	None:intergenic	35.0%
	AGAAGGTTCATTCTCAGTTA+TGG	-	chr3.1:78633906-78633925	None:intergenic	35.0%
	CATTGCAATAGTCCTAAACA+AGG	+	chr3.1:78635279-78635298	MS.gene06255:CDS	35.0%
	CTCAGTTATGGTAGTAACTT+TGG	-	chr3.1:78633894-78633913	None:intergenic	35.0%
	GAATCCTATGTTCTATGACA+TGG	+	chr3.1:78635081-78635100	MS.gene06255:CDS	35.0%
	GTCTTTACAGAATCAAGTAG+AGG	+	chr3.1:78634936-78634955	MS.gene06255:CDS	35.0%
	TTGAGAGCTTACAAACATGT+TGG	-	chr3.1:78635637-78635656	None:intergenic	35.0%
!	CAATTTTTCTACAAGGGCAA+AGG	+	chr3.1:78635385-78635404	MS.gene06255:CDS	35.0%
!	GACACAGATGATAATGTTGA+TGG	-	chr3.1:78635258-78635277	None:intergenic	35.0%
!	TTGAAGCTGAAGTATCCAAA+AGG	-	chr3.1:78635368-78635387	None:intergenic	35.0%
!!!	AGGTTTTTGGTGAAAGAAGA+AGG	-	chr3.1:78633923-78633942	None:intergenic	35.0%
	AAAGGTAGTCATTAGCAGCT+TGG	-	chr3.1:78635350-78635369	None:intergenic	40.0%
	ACCTTCATGACAGCACATAT+AGG	+	chr3.1:78634763-78634782	MS.gene06255:intron	40.0%
	ATGAGTCAACAAGGAAAGGA+TGG	-	chr3.1:78633772-78633791	None:intergenic	40.0%
	ATTAGCAGCTTGGAAGATGT+TGG	-	chr3.1:78635340-78635359	None:intergenic	40.0%
	GCATGAATGATGAGTCAACA+AGG	-	chr3.1:78633781-78633800	None:intergenic	40.0%
	GTCATCAAATGTTGTCTCTG+AGG	+	chr3.1:78635407-78635426	MS.gene06255:CDS	40.0%
	GTTGCCATGTCATAGAACAT+AGG	-	chr3.1:78635088-78635107	None:intergenic	40.0%
	TGTACTATACCCTTTCAGCA+AGG	-	chr3.1:78634536-78634555	None:intergenic	40.0%
	TTGTAACCAGGTAACTGGAA+AGG	+	chr3.1:78634879-78634898	MS.gene06255:intron	40.0%
!	AACAGACCTAGACACTCTTT+TGG	+	chr3.1:78635105-78635124	MS.gene06255:CDS	40.0%
!	AGCTGCTAATGACTACCTTT+TGG	+	chr3.1:78635350-78635369	MS.gene06255:CDS	40.0%
!	ATGGATTGTAACCAGGTAAC+TGG	+	chr3.1:78634874-78634893	MS.gene06255:intron	40.0%
!	GTGAGGGTTAGTATTGTTTC+TGG	-	chr3.1:78634038-78634057	None:intergenic	40.0%
!	GTTCTGGACAAGCTTGAAAA+TGG	+	chr3.1:78633962-78633981	MS.gene06255:CDS	40.0%
!!	AGACTGTGGACTAAGGTTTT+TGG	-	chr3.1:78633936-78633955	None:intergenic	40.0%
!!	CACTCTTTTGGTTAGACAAG+AGG	+	chr3.1:78635117-78635136	MS.gene06255:CDS	40.0%
!!	GCAAAAGTTGTGCCTTGTTT+AGG	-	chr3.1:78635294-78635313	None:intergenic	40.0%
!!	GTCTAACCAAAAGAGTGTCT+AGG	-	chr3.1:78635114-78635133	None:intergenic	40.0%
!!	TTCAGGACGGTAGTCATTTT+TGG	+	chr3.1:78635515-78635534	MS.gene06255:intron	40.0%
!!!	TCAGGACGGTAGTCATTTTT+GGG	+	chr3.1:78635516-78635535	MS.gene06255:intron	40.0%
	ATAGTCCTACGGAGAAGAAG+AGG	+	chr3.1:78634008-78634027	MS.gene06255:CDS	45.0%
	GACAGGATAATGCAGCAGAA+GGG	+	chr3.1:78634370-78634389	MS.gene06255:intron	45.0%
	GCCTATATGTGCTGTCATGA+AGG	-	chr3.1:78634767-78634786	None:intergenic	45.0%
	TAAGAGCCTTTCCAGTTACC+TGG	-	chr3.1:78634888-78634907	None:intergenic	45.0%
	TGAAGAGGGTGAAACTGTGA+AGG	-	chr3.1:78633836-78633855	None:intergenic	45.0%
	TGACAGGATAATGCAGCAGA+AGG	+	chr3.1:78634369-78634388	MS.gene06255:intron	45.0%
	TTCAAGCTTGTCCAGAACCA+TGG	-	chr3.1:78633960-78633979	None:intergenic	45.0%
!	GAAGATGTTGGTTGGAGTCA+TGG	-	chr3.1:78635328-78635347	None:intergenic	45.0%
!!!	GACGGTAGTCATTTTTGGGA+GGG	+	chr3.1:78635520-78635539	MS.gene06255:intron	45.0%
!!	TCATAAAATATAATTATTTA+TGG	+	chr3.1:78634855-78634874	MS.gene06255:intron	5.0%
	AAGTTGCAGCAACTTGTGCC+AGG	+	chr3.1:78634716-78634735	MS.gene06255:CDS	50.0%
	ACGTGGATATAGCCAGTTGG+GGG	-	chr3.1:78634477-78634496	None:intergenic	50.0%
	ACTTGTGCCAGGCTGTGATA+AGG	+	chr3.1:78634727-78634746	MS.gene06255:CDS	50.0%
	AGAGGGTGAAACTGTGAAGG+TGG	-	chr3.1:78633833-78633852	None:intergenic	50.0%
	ATACCCTTTCAGCAAGGCTG+TGG	-	chr3.1:78634530-78634549	None:intergenic	50.0%
	ATAGCCACAGCCTTGCTGAA+AGG	+	chr3.1:78634523-78634542	MS.gene06255:CDS	50.0%
	ATATCCACGTCCGAGCAAGA+AGG	+	chr3.1:78634487-78634506	MS.gene06255:CDS	50.0%
	CATGTGTGTGTGTGTGTGCA+TGG	-	chr3.1:78634287-78634306	None:intergenic	50.0%
	CCTTAGTCCACAGTCTTCCA+TGG	+	chr3.1:78633940-78633959	MS.gene06255:CDS	50.0%
	GACGTGGATATAGCCAGTTG+GGG	-	chr3.1:78634478-78634497	None:intergenic	50.0%
	GCAGCTTGGAAGATGTTGGT+TGG	-	chr3.1:78635336-78635355	None:intergenic	50.0%
	GGACGTGGATATAGCCAGTT+GGG	-	chr3.1:78634479-78634498	None:intergenic	50.0%
	GTTCTCCTCTTCTTCTCCGT+AGG	-	chr3.1:78634016-78634035	None:intergenic	50.0%
	TAGCCACAGCCTTGCTGAAA+GGG	+	chr3.1:78634524-78634543	MS.gene06255:CDS	50.0%
	TATCCACGTCCGAGCAAGAA+GGG	+	chr3.1:78634488-78634507	MS.gene06255:CDS	50.0%
	TCCACAGTCTTCCATGGTTC+TGG	+	chr3.1:78633946-78633965	MS.gene06255:CDS	50.0%
	TCCAGAACCATGGAAGACTG+TGG	-	chr3.1:78633950-78633969	None:intergenic	50.0%
	TGAAACTGTGAAGGTGGAGG+AGG	-	chr3.1:78633827-78633846	None:intergenic	50.0%
	TGACCTACCTTATCACAGCC+TGG	-	chr3.1:78634737-78634756	None:intergenic	50.0%
!	CCATGGAAGACTGTGGACTA+AGG	-	chr3.1:78633943-78633962	None:intergenic	50.0%
!!!	GGACGGTAGTCATTTTTGGG+AGG	+	chr3.1:78635519-78635538	MS.gene06255:intron	50.0%
	AACTGTGAAGGTGGAGGAGG+AGG	-	chr3.1:78633824-78633843	None:intergenic	55.0%
	AGCAAGGCTGTGGCTATCAG+TGG	-	chr3.1:78634520-78634539	None:intergenic	55.0%
	ATCCACGTCCGAGCAAGAAG+GGG	+	chr3.1:78634489-78634508	MS.gene06255:CDS	55.0%
	CGGACGTGGATATAGCCAGT+TGG	-	chr3.1:78634480-78634499	None:intergenic	55.0%
	GGGTGAAACTGTGAAGGTGG+AGG	-	chr3.1:78633830-78633849	None:intergenic	55.0%
	GTGCCAGGCTGTGATAAGGT+AGG	+	chr3.1:78634731-78634750	MS.gene06255:intron	55.0%
	TGTTTGGATGAGCCCCCAAC+TGG	+	chr3.1:78634462-78634481	MS.gene06255:CDS	55.0%
	TGGCCTGACCCCTTCTTGCT+CGG	-	chr3.1:78634500-78634519	None:intergenic	60.0%
	CGTCCGAGCAAGAAGGGGTC+AGG	+	chr3.1:78634494-78634513	MS.gene06255:CDS	65.0%
	GACCCCTTCTTGCTCGGACG+TGG	-	chr3.1:78634494-78634513	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	78633746	78635749	78633746	ID=MS.gene06255
chr3.1	mRNA	78633746	78635749	78633746	ID=MS.gene06255.t1;Parent=MS.gene06255
chr3.1	exon	78633746	78634063	78633746	ID=MS.gene06255.t1.exon1;Parent=MS.gene06255.t1
chr3.1	CDS	78633746	78634063	78633746	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78634375	78634545	78634375	ID=MS.gene06255.t1.exon2;Parent=MS.gene06255.t1
chr3.1	CDS	78634375	78634545	78634375	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78634683	78634748	78634683	ID=MS.gene06255.t1.exon3;Parent=MS.gene06255.t1
chr3.1	CDS	78634683	78634748	78634683	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78634889	78634957	78634889	ID=MS.gene06255.t1.exon4;Parent=MS.gene06255.t1
chr3.1	CDS	78634889	78634957	78634889	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78635055	78635138	78635055	ID=MS.gene06255.t1.exon5;Parent=MS.gene06255.t1
chr3.1	CDS	78635055	78635138	78635055	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78635234	78635428	78635234	ID=MS.gene06255.t1.exon6;Parent=MS.gene06255.t1
chr3.1	CDS	78635234	78635428	78635234	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1
chr3.1	exon	78635741	78635749	78635741	ID=MS.gene06255.t1.exon7;Parent=MS.gene06255.t1
chr3.1	CDS	78635741	78635749	78635741	ID=cds.MS.gene06255.t1;Parent=MS.gene06255.t1

Gene Sequence

>MS.gene06255

ATGGCAGCTTTTTCATATCAATACCATCCTTTCCTTGTTGACTCATCATTCATGCTCAACATGAACACTGCTTCTCCTCCTCCTCCACCTTCACAGTTTCACCCTCTTCATCAAGATATTATTAACTGTGTTGATCAACAAAGCTCCAAAGTTACTACCATAACTGAGAATGAACCTTCTTCTTTCACCAAAAACCTTAGTCCACAGTCTTCCATGGTTCTGGACAAGCTTGAAAATGGTGATGAGCAAGTTACTCAGAAAAATAGTCCTACGGAGAAGAAGAGGAGAACCAGAAACAATACTAACCCTCACTCTCATGTAATAATATTAATTGTTTCTGTTTCCATCTCCATCATATATATCACTGCTAGAAAAAACTAAAATTATCAATCAAAATTATAGACAAAAATATAAAATTCCTATGACAGAAGAATTAGAGATATGTTTAATGTTTTCCTTATTTTAAGAAAACTTGTGGATAATTTTTTTGGTAATGTATTTTTAGCACTTTTTTTATACTATGGTTTCATTAATTAAGCCATGCACACACACACACACATGTGTTTTGTTCCTGTTATGAATTGAGTTGATTATTTTGTACTGAATTTGTTAGTAATTAAATATGACAGGATAATGCAGCAGAAGGGAAAAACAAGAAACTGAAGAAAAACAAAGAAGATGAGAAGGCAAAAGCAGAGAAAAAAGATCAGAGAAAGTGTTTGGATGAGCCCCCAACTGGCTATATCCACGTCCGAGCAAGAAGGGGTCAGGCCACTGATAGCCACAGCCTTGCTGAAAGGGTATAGTACATTACTAGTATTTTCATTTCATGTGGGGTCCACTTGTATGATACAATTCCAAAAATGTCCCTTTTAGTTATCTTCATGTCTCATTCTGAATGTTGCAAATTGAATTAAATTGGATGAATTATATTCAGGTAAGAAGAGAGAAAATAAGTGAAAGGATGAAGAAGTTGCAGCAACTTGTGCCAGGCTGTGATAAGGTAGGTCAAGTAAAACCTTCATGACAGCACATATAGGCCCATGCAATTAAAAGTCTCAAAACTATACTGTTGAAAATAATATCTTTAATAATGAACATTCTATTTTTCATAAAATATAATTATTTATGGATTGTAACCAGGTAACTGGAAAGGCTCTTATGTTAGATGAAATAATCAATTATGTTCAGTCTTTACAGAATCAAGTAGAGGTAACATCAATATTCTCAAGCTGTTTTGTGGTTATTTATTTACTTAAAATGTATAAATATACTAATTAACAAGCTATATGCTCTCTATTTTTTTCAGTTCTTGTCAATGAAACTTACTTCTGTGAATCCTATGTTCTATGACATGGCAACAGACCTAGACACTCTTTTGGTTAGACAAGAGGTGAGTAAGAAAATTCAGTGATATATGATGATTCACATGTTAGGTCTAATAATGAAATTAACTAACTAACTTAGTATTTTTGAATGAATTATCAGAAATTAAATAACTTGGCATCACCATCAACATTATCATCTGTGTCACATTGCAATAGTCCTAAACAAGGCACAACTTTTGCTGATACAACAACCATGACTCCAACCAACATCTTCCAAGCTGCTAATGACTACCTTTTGGATACTTCAGCTTCAATTTTTCTACAAGGGCAAAGGTCATCAAATGTTGTCTCTGAGGTATAAAAACACAAATAACCAAAAAAATGATTTAATCCTAAAATGGATGTAATTATTCTTTTCTTATTCTGACAAAAAATAACATTTTCAGGACGGTAGTCATTTTTGGGAGGGACAAAAGTTTCTTCATTCACATGGATTCAACAGCAACTTATGTTCCTTTCATTAATTTAAAATTCACAGATGTGCATGCCCTACAAAACTACCAACATGTTTGTAAGCTCTCAAATCCTGTCTCTTATATTATATAATTTAATTTACTTAATAATACTTGGTTTCTAATTCTAATACTTATAATTTTTTTTTCTTCAGGATTTTTAG

Protein sequence

>MS.gene06255.t1

MAAFSYQYHPFLVDSSFMLNMNTASPPPPPSQFHPLHQDIINCVDQQSSKVTTITENEPSSFTKNLSPQSSMVLDKLENGDEQVTQKNSPTEKKRRTRNNTNPHSHDNAAEGKNKKLKKNKEDEKAKAEKKDQRKCLDEPPTGYIHVRARRGQATDSHSLAERVRREKISERMKKLQQLVPGCDKVTGKALMLDEIINYVQSLQNQVEFLSMKLTSVNPMFYDMATDLDTLLVRQEKLNNLASPSTLSSVSHCNSPKQGTTFADTTTMTPTNIFQAANDYLLDTSASIFLQGQRSSNVVSEDF