AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062717.t1 MTR_1g021925 97.391 345 9 0 1 345 39 383 0.0 701
MS.gene062717.t1 MTR_4g069140 62.691 327 121 1 3 329 41 366 9.84e-154 436
MS.gene062717.t1 MTR_1g052685 35.931 231 147 1 60 289 84 314 6.01e-46 161
MS.gene062717.t1 MTR_7g092280 32.584 267 166 3 59 311 83 349 4.46e-42 150
MS.gene062717.t1 MTR_2g097980 36.199 221 125 3 59 279 83 287 9.99e-39 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062717.t1 AT1G75020 65.549 328 113 0 1 328 38 365 8.88e-168 473
MS.gene062717.t1 AT1G75020 65.549 328 113 0 1 328 38 365 8.88e-168 473
MS.gene062717.t1 AT3G18850 62.952 332 122 1 1 331 33 364 1.10e-159 452
MS.gene062717.t1 AT3G18850 62.952 332 122 1 1 331 33 364 1.10e-159 452
MS.gene062717.t1 AT3G18850 62.952 332 122 1 1 331 33 364 1.10e-159 452
MS.gene062717.t1 AT3G18850 62.952 332 122 1 1 331 33 364 1.10e-159 452
MS.gene062717.t1 AT3G18850 64.198 324 115 1 9 331 18 341 3.68e-159 449
MS.gene062717.t1 AT3G18850 57.798 218 91 1 115 331 16 233 2.18e-92 276
MS.gene062717.t1 AT3G57650 33.969 262 172 1 59 319 82 343 8.05e-47 163
MS.gene062717.t1 AT1G51260 30.667 300 190 6 32 327 53 338 7.35e-39 142

Find 58 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTTAGTTTCAGAAAT+TGG 0.158348 1.4:+2539619 None:intergenic
ACAAAATCTTTCAACATTTC+AGG 0.190346 1.4:-2540935 MS.gene062717:CDS
AGGATCCGAAAGATCCTTTA+TGG 0.230304 1.4:-2540915 MS.gene062717:CDS
CAGAAGTCCACTTGCATATT+CGG 0.261346 1.4:-2539663 MS.gene062717:CDS
AAAGCAATTGGTCCTTGATT+TGG 0.267935 1.4:+2539575 None:intergenic
GCATGTACATAATCTGTTAA+AGG 0.268484 1.4:+2539343 None:intergenic
CTGAAACTAAAGCTGCTTCT+TGG 0.278746 1.4:-2539612 MS.gene062717:CDS
AAGGCAGTATCATTCTTCTT+TGG 0.293370 1.4:-2541249 MS.gene062717:CDS
TGGTTGGGGATTTCAGATTC+TGG 0.302482 1.4:-2541007 MS.gene062717:CDS
CTTTCGCCGCTTGCAGTTGT+AGG 0.315648 1.4:-2541303 MS.gene062717:CDS
CCAATTGCTTTCTGATTTCA+AGG 0.330496 1.4:-2539563 MS.gene062717:CDS
TAAAGCTGCTTCTTGGCTAA+TGG 0.348528 1.4:-2539605 MS.gene062717:CDS
TCATTGCAGTGGAGAGGAAA+TGG 0.367397 1.4:-2540981 MS.gene062717:CDS
GTTTCATTAAGCTGCTCTTA+AGG 0.367606 1.4:+2541040 None:intergenic
CTTTCTGATTTCAAGGTTCA+AGG 0.386029 1.4:-2539556 MS.gene062717:CDS
TATCATTCTTCTTTGGACTT+TGG 0.386228 1.4:-2541242 MS.gene062717:CDS
TACGTATAATCGGTTCCTTC+GGG 0.405431 1.4:+2540880 None:intergenic
ATCACAGAACTGAGGTTGAT+TGG 0.411546 1.4:-2541119 MS.gene062717:CDS
GATAGCCATAAAGGATCTTT+CGG 0.432489 1.4:+2540910 None:intergenic
GTACGTATAATCGGTTCCTT+CGG 0.443486 1.4:+2540879 None:intergenic
CTATCACGGTAAAAGTGAAT+AGG 0.446000 1.4:+2539485 None:intergenic
CCTTGAAATCAGAAAGCAAT+TGG 0.447220 1.4:+2539563 None:intergenic
ATCAACCTCAGTTCTGTGAT+TGG 0.449343 1.4:+2541122 None:intergenic
GTGGGATCTTGCTCTTAGAA+AGG 0.458545 1.4:-2541088 MS.gene062717:CDS
AAAGGGGTTTCACACTTGCT+TGG 0.459653 1.4:-2540533 MS.gene062717:CDS
TTGGACTTTGGTTATCTATG+TGG 0.460047 1.4:-2541230 MS.gene062717:CDS
TTCGAGGTAGTACGTATAAT+CGG 0.463252 1.4:+2540870 None:intergenic
GAAATTGGAATCTCCTCTAC+AGG 0.473130 1.4:+2539634 None:intergenic
TGGGATCTTGCTCTTAGAAA+GGG 0.490923 1.4:-2541087 MS.gene062717:CDS
TTATCAGTATTCGTTATAGT+AGG 0.491260 1.4:-2541272 MS.gene062717:CDS
GGAAACTCTACAAGGCTCAT+TGG 0.496811 1.4:-2540512 MS.gene062717:CDS
CGCTTGCAGTTGTAGGATTG+CGG 0.503380 1.4:-2541296 MS.gene062717:CDS
GATTCTGGAATTCATTGCAG+TGG 0.510533 1.4:-2540992 MS.gene062717:CDS
CTACAAGGCTCATTGGATGC+AGG 0.515831 1.4:-2540505 MS.gene062717:intron
CATTGCAGTGGAGAGGAAAT+GGG 0.521317 1.4:-2540980 MS.gene062717:CDS
GGTTGATTGGATGTACTTGT+GGG 0.523971 1.4:-2541106 MS.gene062717:CDS
CGCAATCCTACAACTGCAAG+CGG 0.528807 1.4:+2541297 None:intergenic
AATGGATGCATTCCAAATCA+AGG 0.541765 1.4:-2539587 MS.gene062717:CDS
GCTCTTAGAAAGGGAAGACT+AGG 0.543581 1.4:-2541078 MS.gene062717:CDS
CCCTAACCAACAAAATGAAG+TGG 0.545633 1.4:-2539527 MS.gene062717:CDS
ATATGCAAGTGGACTTCTGA+AGG 0.550458 1.4:+2539667 None:intergenic
AGTAATACATTTGTCAGCAC+AGG 0.552689 1.4:+2540565 None:intergenic
CAGTATTCGTTATAGTAGGA+AGG 0.557462 1.4:-2541268 MS.gene062717:CDS
TGGGGATAGTATTCCGATGA+GGG 0.559784 1.4:-2541163 MS.gene062717:CDS
AAAACATTGTCTAGAAAGGA+AGG 0.565471 1.4:+2539700 None:intergenic
AGGTTGATTGGATGTACTTG+TGG 0.569937 1.4:-2541107 MS.gene062717:CDS
AGTAACACACGTTCCCTCAT+CGG 0.578657 1.4:+2541150 None:intergenic
CAAGGTAAAATAAGTAAACA+AGG 0.586845 1.4:+2539450 None:intergenic
ATCGGGTTCAAGTTATATGT+AGG 0.586979 1.4:-2539418 MS.gene062717:CDS
TGGAATTCATTGCAGTGGAG+AGG 0.600003 1.4:-2540987 MS.gene062717:CDS
CTGGGGATAGTATTCCGATG+AGG 0.613140 1.4:-2541164 MS.gene062717:CDS
ACTTGCTTGGAAACTCTACA+AGG 0.626873 1.4:-2540520 MS.gene062717:CDS
GAACCCGATGTGAGAAAACA+AGG 0.629551 1.4:+2539432 None:intergenic
TATTCGGCGTATTCCTGTAG+AGG 0.633768 1.4:-2539647 MS.gene062717:CDS
GGCGGTAAAACAAACTATCA+CGG 0.635114 1.4:+2539471 None:intergenic
GAATACGCCGAATATGCAAG+TGG 0.679908 1.4:+2539656 None:intergenic
TATCGCCAATCACAGAACTG+AGG 0.748204 1.4:-2541127 MS.gene062717:CDS
GGTAAAATAAGTAAACAAGG+CGG 0.784862 1.4:+2539453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAATATTTGATACT+CGG - chr1.4:2540167-2540186 MS.gene062717:intron 10.0%
!! ATAATATATTGTCTATTTGT+CGG + chr1.4:2539949-2539968 None:intergenic 15.0%
!! ATGTCAAATAAAATAATTTG+TGG + chr1.4:2539862-2539881 None:intergenic 15.0%
!! ATATGGAATATACACAAATT+CGG + chr1.4:2540695-2540714 None:intergenic 20.0%
!!! TTTATCTTTTCGAATAGAAA+GGG + chr1.4:2539423-2539442 None:intergenic 20.0%
! ACAAAATCTTTCAACATTTC+AGG - chr1.4:2539692-2539711 MS.gene062717:CDS 25.0%
! AGAAACTGCAAAGATATATT+TGG + chr1.4:2540341-2540360 None:intergenic 25.0%
! AGTCAAAATATTGCTCATAA+TGG + chr1.4:2539896-2539915 None:intergenic 25.0%
! CAAGGTAAAATAAGTAAACA+AGG + chr1.4:2541180-2541199 None:intergenic 25.0%
! CAATATATTATTCACTTCAG+TGG - chr1.4:2539958-2539977 MS.gene062717:intron 25.0%
! GTATTACTTCCAAAAACAAA+GGG - chr1.4:2540077-2540096 MS.gene062717:intron 25.0%
! GTCAATTCATAATGACTTTA+TGG - chr1.4:2540189-2540208 MS.gene062717:intron 25.0%
! TATTACTTCCAAAAACAAAG+GGG - chr1.4:2540078-2540097 MS.gene062717:intron 25.0%
! TGTATTACTTCCAAAAACAA+AGG - chr1.4:2540076-2540095 MS.gene062717:intron 25.0%
! TTACATTATGTGATCGAAAT+CGG - chr1.4:2540475-2540494 MS.gene062717:intron 25.0%
! TTAGAAAAGAAAAGAGACAA+GGG + chr1.4:2540821-2540840 None:intergenic 25.0%
! TTATCAGTATTCGTTATAGT+AGG - chr1.4:2539355-2539374 MS.gene062717:CDS 25.0%
! TTTAGAAAAGAAAAGAGACA+AGG + chr1.4:2540822-2540841 None:intergenic 25.0%
!! AAAACTAAAGTTGTCTTTTC+TGG - chr1.4:2539444-2539463 MS.gene062717:CDS 25.0%
!! AAACTAAAGTTGTCTTTTCT+GGG - chr1.4:2539445-2539464 MS.gene062717:CDS 25.0%
!! GTTTATCTTTTCGAATAGAA+AGG + chr1.4:2539424-2539443 None:intergenic 25.0%
!!! TGTTCAACAATATTTTGACA+TGG - chr1.4:2540770-2540789 MS.gene062717:intron 25.0%
!!! TTAATGAAACTACCGATTTT+TGG - chr1.4:2539600-2539619 MS.gene062717:CDS 25.0%
AAAACATTGTCTAGAAAGGA+AGG + chr1.4:2540930-2540949 None:intergenic 30.0%
ACCAAAAACATTGTCTAGAA+AGG + chr1.4:2540934-2540953 None:intergenic 30.0%
ATATTCCATATCATTCCCTA+GGG - chr1.4:2540704-2540723 MS.gene062717:intron 30.0%
GCATGTACATAATCTGTTAA+AGG + chr1.4:2541287-2541306 None:intergenic 30.0%
GGTAAAATAAGTAAACAAGG+CGG + chr1.4:2541177-2541196 None:intergenic 30.0%
TAGAAAAGAAAAGAGACAAG+GGG + chr1.4:2540820-2540839 None:intergenic 30.0%
TATATTCCATATCATTCCCT+AGG - chr1.4:2540703-2540722 MS.gene062717:intron 30.0%
TATCATTCTTCTTTGGACTT+TGG - chr1.4:2539385-2539404 MS.gene062717:CDS 30.0%
! AACTAAAGTTGTCTTTTCTG+GGG - chr1.4:2539446-2539465 MS.gene062717:CDS 30.0%
! AGAATTCCACTTCATTTTGT+TGG + chr1.4:2541109-2541128 None:intergenic 30.0%
! TCCTTTCTAGACAATGTTTT+TGG - chr1.4:2540930-2540949 MS.gene062717:CDS 30.0%
!!! TTTACCTTGTTTTCTCACAT+CGG - chr1.4:2541191-2541210 MS.gene062717:CDS 30.0%
AAAGCAATTGGTCCTTGATT+TGG + chr1.4:2541055-2541074 None:intergenic 35.0%
AAGGCAGTATCATTCTTCTT+TGG - chr1.4:2539378-2539397 MS.gene062717:CDS 35.0%
AATGGATGCATTCCAAATCA+AGG - chr1.4:2541040-2541059 MS.gene062717:CDS 35.0%
AGTAATACATTTGTCAGCAC+AGG + chr1.4:2540065-2540084 None:intergenic 35.0%
CAGTATTCGTTATAGTAGGA+AGG - chr1.4:2539359-2539378 MS.gene062717:CDS 35.0%
CCAATTGCTTTCTGATTTCA+AGG - chr1.4:2541064-2541083 MS.gene062717:CDS 35.0%
CCTTGAAATCAGAAAGCAAT+TGG + chr1.4:2541067-2541086 None:intergenic 35.0%
CTATCACGGTAAAAGTGAAT+AGG + chr1.4:2541145-2541164 None:intergenic 35.0%
CTTTCTGATTTCAAGGTTCA+AGG - chr1.4:2541071-2541090 MS.gene062717:CDS 35.0%
GAAGGTTCAAATTTCAAGCT+CGG - chr1.4:2540550-2540569 MS.gene062717:CDS 35.0%
GATAGCCATAAAGGATCTTT+CGG + chr1.4:2539720-2539739 None:intergenic 35.0%
GGAAAAAGAGATAGCCATAA+AGG + chr1.4:2539729-2539748 None:intergenic 35.0%
GTTTCATTAAGCTGCTCTTA+AGG + chr1.4:2539590-2539609 None:intergenic 35.0%
TACAGGGTAAATTCAAAGCA+AGG + chr1.4:2540401-2540420 None:intergenic 35.0%
TCAAAATCTGCATTCAAACC+TGG - chr1.4:2540599-2540618 MS.gene062717:CDS 35.0%
TCTCTGTTACCAAAATGCAA+TGG - chr1.4:2540235-2540254 MS.gene062717:intron 35.0%
TGAAATCCCCAACCAAAAAT+CGG + chr1.4:2539615-2539634 None:intergenic 35.0%
TTCAGTTCCAAACGTATGTT+CGG - chr1.4:2540295-2540314 MS.gene062717:intron 35.0%
TTCGAGGTAGTACGTATAAT+CGG + chr1.4:2539760-2539779 None:intergenic 35.0%
TTGGACTTTGGTTATCTATG+TGG - chr1.4:2539397-2539416 MS.gene062717:CDS 35.0%
TTTGGATCCAAACATACGTT+TGG + chr1.4:2540323-2540342 None:intergenic 35.0%
! AAACCAAGTTTGCTTTCACT+AGG - chr1.4:2540747-2540766 MS.gene062717:intron 35.0%
! ATCGGGTTCAAGTTATATGT+AGG - chr1.4:2541209-2541228 MS.gene062717:CDS 35.0%
! GCAGCTTTAGTTTCAGAAAT+TGG + chr1.4:2541011-2541030 None:intergenic 35.0%
! TTACCAAAATGCAATGGACT+TGG - chr1.4:2540241-2540260 MS.gene062717:intron 35.0%
! TTGCTTTGAATTTACCCTGT+AGG - chr1.4:2540400-2540419 MS.gene062717:intron 35.0%
!! CAAAAATTTGCTGCTGAAGT+TGG - chr1.4:2540035-2540054 MS.gene062717:intron 35.0%
!! CATTTTGTTGGTTAGGGAAA+TGG + chr1.4:2541097-2541116 None:intergenic 35.0%
!! CCACTTCATTTTGTTGGTTA+GGG + chr1.4:2541103-2541122 None:intergenic 35.0%
!! TCCACTTCATTTTGTTGGTT+AGG + chr1.4:2541104-2541123 None:intergenic 35.0%
!!! AAACTACCGATTTTTGGTTG+GGG - chr1.4:2539606-2539625 MS.gene062717:CDS 35.0%
!!! CTCTGTGTTTTTTGCTTTAG+GGG - chr1.4:2540426-2540445 MS.gene062717:intron 35.0%
!!! GAAACTACCGATTTTTGGTT+GGG - chr1.4:2539605-2539624 MS.gene062717:CDS 35.0%
!!! GCTCTGTGTTTTTTGCTTTA+GGG - chr1.4:2540425-2540444 MS.gene062717:intron 35.0%
!!! TGAAACTACCGATTTTTGGT+TGG - chr1.4:2539604-2539623 MS.gene062717:CDS 35.0%
!!! TGCTCTGTGTTTTTTGCTTT+AGG - chr1.4:2540424-2540443 MS.gene062717:intron 35.0%
!!! TTACCTTGTTTTCTCACATC+GGG - chr1.4:2541192-2541211 MS.gene062717:CDS 35.0%
AAAAGAGACAAGGGGAAAGT+TGG + chr1.4:2540812-2540831 None:intergenic 40.0%
ACACCTAGTGAAAGCAAACT+TGG + chr1.4:2540753-2540772 None:intergenic 40.0%
ACTTGCTTGGAAACTCTACA+AGG - chr1.4:2540107-2540126 MS.gene062717:intron 40.0%
AGGATCCGAAAGATCCTTTA+TGG - chr1.4:2539712-2539731 MS.gene062717:CDS 40.0%
ATCAACCTCAGTTCTGTGAT+TGG + chr1.4:2539508-2539527 None:intergenic 40.0%
CAGAAGTCCACTTGCATATT+CGG - chr1.4:2540964-2540983 MS.gene062717:CDS 40.0%
CATGCATCCAGTTAGCATAT+TGG + chr1.4:2540651-2540670 None:intergenic 40.0%
CCCTAACCAACAAAATGAAG+TGG - chr1.4:2541100-2541119 MS.gene062717:CDS 40.0%
CTGAAACTAAAGCTGCTTCT+TGG - chr1.4:2541015-2541034 MS.gene062717:CDS 40.0%
GAAATTGGAATCTCCTCTAC+AGG + chr1.4:2540996-2541015 None:intergenic 40.0%
GGCGGTAAAACAAACTATCA+CGG + chr1.4:2541159-2541178 None:intergenic 40.0%
GGTCGTTGTTCGATCAAAAT+CGG - chr1.4:2540528-2540547 MS.gene062717:CDS 40.0%
GGTTGATTGGATGTACTTGT+GGG - chr1.4:2539521-2539540 MS.gene062717:CDS 40.0%
GTTGTTCGATCAAAATCGGA+AGG - chr1.4:2540532-2540551 MS.gene062717:CDS 40.0%
TAAAGCTGCTTCTTGGCTAA+TGG - chr1.4:2541022-2541041 MS.gene062717:CDS 40.0%
TGGGATCTTGCTCTTAGAAA+GGG - chr1.4:2539540-2539559 MS.gene062717:CDS 40.0%
TTCGGATCCAAACGTATGTT+TGG - chr1.4:2540313-2540332 MS.gene062717:intron 40.0%
TTTGGATCCGAACATACGTT+TGG + chr1.4:2540305-2540324 None:intergenic 40.0%
! AAAAACACAGAGCATCCTAC+AGG + chr1.4:2540418-2540437 None:intergenic 40.0%
! AAAACACAGAGCATCCTACA+GGG + chr1.4:2540417-2540436 None:intergenic 40.0%
! AAGCCAAGTCCATTGCATTT+TGG + chr1.4:2540247-2540266 None:intergenic 40.0%
! AGGTTGATTGGATGTACTTG+TGG - chr1.4:2539520-2539539 MS.gene062717:CDS 40.0%
! ATAACACGATGCGCGTATTT+TGG + chr1.4:2539925-2539944 None:intergenic 40.0%
! ATATGCAAGTGGACTTCTGA+AGG + chr1.4:2540963-2540982 None:intergenic 40.0%
! ATCACAGAACTGAGGTTGAT+TGG - chr1.4:2539508-2539527 MS.gene062717:CDS 40.0%
! CTTTAGGGGTGGATTTCTTT+AGG - chr1.4:2540440-2540459 MS.gene062717:intron 40.0%
! GTACGTATAATCGGTTCCTT+CGG + chr1.4:2539751-2539770 None:intergenic 40.0%
! TAACACGATGCGCGTATTTT+GGG + chr1.4:2539924-2539943 None:intergenic 40.0%
! TACGTATAATCGGTTCCTTC+GGG + chr1.4:2539750-2539769 None:intergenic 40.0%
!! GATTCTGGAATTCATTGCAG+TGG - chr1.4:2539635-2539654 MS.gene062717:CDS 40.0%
!! GATTTTGATCGAACAACGAC+CGG + chr1.4:2540529-2540548 None:intergenic 40.0%
!!! GTGTGAAACCCCTTTGTTTT+TGG + chr1.4:2540089-2540108 None:intergenic 40.0%
!!! TGTGTTTTTTGCTTTAGGGG+TGG - chr1.4:2540429-2540448 MS.gene062717:intron 40.0%
AAAGGGGTTTCACACTTGCT+TGG - chr1.4:2540094-2540113 MS.gene062717:intron 45.0%
AACTAGCATTCTGCTTCCCT+AGG + chr1.4:2540723-2540742 None:intergenic 45.0%
ACGACCACCAATATGCTAAC+TGG - chr1.4:2540641-2540660 MS.gene062717:intron 45.0%
ACTAGCATTCTGCTTCCCTA+GGG + chr1.4:2540722-2540741 None:intergenic 45.0%
AGTAACACACGTTCCCTCAT+CGG + chr1.4:2539480-2539499 None:intergenic 45.0%
CATTGCAGTGGAGAGGAAAT+GGG - chr1.4:2539647-2539666 MS.gene062717:CDS 45.0%
GAACCCGATGTGAGAAAACA+AGG + chr1.4:2541198-2541217 None:intergenic 45.0%
GAATACGCCGAATATGCAAG+TGG + chr1.4:2540974-2540993 None:intergenic 45.0%
GCATCCAGTTAGCATATTGG+TGG + chr1.4:2540648-2540667 None:intergenic 45.0%
GCTCTTAGAAAGGGAAGACT+AGG - chr1.4:2539549-2539568 MS.gene062717:CDS 45.0%
GGAAACTCTACAAGGCTCAT+TGG - chr1.4:2540115-2540134 MS.gene062717:intron 45.0%
GTCGTTGGATCGAGATCAAA+CGG + chr1.4:2540626-2540645 None:intergenic 45.0%
GTGGGATCTTGCTCTTAGAA+AGG - chr1.4:2539539-2539558 MS.gene062717:CDS 45.0%
TATCGCCAATCACAGAACTG+AGG - chr1.4:2539500-2539519 MS.gene062717:CDS 45.0%
TATTCGGCGTATTCCTGTAG+AGG - chr1.4:2540980-2540999 MS.gene062717:CDS 45.0%
TCATTGCAGTGGAGAGGAAA+TGG - chr1.4:2539646-2539665 MS.gene062717:CDS 45.0%
TGCTTCCCTAGGGAATGATA+TGG + chr1.4:2540712-2540731 None:intergenic 45.0%
TGGAATTCATTGCAGTGGAG+AGG - chr1.4:2539640-2539659 MS.gene062717:CDS 45.0%
TGGGGATAGTATTCCGATGA+GGG - chr1.4:2539464-2539483 MS.gene062717:CDS 45.0%
TGGTTGGGGATTTCAGATTC+TGG - chr1.4:2539620-2539639 MS.gene062717:CDS 45.0%
! GTTAGCATATTGGTGGTCGT+TGG + chr1.4:2540641-2540660 None:intergenic 45.0%
! TGGCTATCTCTTTTTCCCGA+AGG - chr1.4:2539732-2539751 MS.gene062717:CDS 45.0%
!! CAAATATTAAAAAAATTAAA+AGG + chr1.4:2540162-2540181 None:intergenic 5.0%
ACCTCGAATCCTTCTCAGCT+CGG - chr1.4:2539772-2539791 MS.gene062717:intron 50.0%
AGATGAGATCCGAGCTGAGA+AGG + chr1.4:2539784-2539803 None:intergenic 50.0%
CGCAATCCTACAACTGCAAG+CGG + chr1.4:2539333-2539352 None:intergenic 50.0%
CGCTTGCAGTTGTAGGATTG+CGG - chr1.4:2539331-2539350 MS.gene062717:CDS 50.0%
CTACAAGGCTCATTGGATGC+AGG - chr1.4:2540122-2540141 MS.gene062717:intron 50.0%
CTGGGGATAGTATTCCGATG+AGG - chr1.4:2539463-2539482 MS.gene062717:CDS 50.0%
ATTCCACGCATTCAGCACGC+CGG - chr1.4:2540507-2540526 MS.gene062717:CDS 55.0%
CTTTCGCCGCTTGCAGTTGT+AGG - chr1.4:2539324-2539343 MS.gene062717:CDS 55.0%
GGATCGAGATCAAACGGTCC+AGG + chr1.4:2540620-2540639 None:intergenic 55.0%
TCCGAGCTGAGAAGGATTCG+AGG + chr1.4:2539776-2539795 None:intergenic 55.0%
CGACCGGCGTGCTGAATGCG+TGG + chr1.4:2540513-2540532 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 2539309 2541340 2539309 ID=MS.gene062717
chr1.4 mRNA 2539309 2541340 2539309 ID=MS.gene062717.t1;Parent=MS.gene062717
chr1.4 exon 2540883 2541340 2540883 ID=MS.gene062717.t1.exon1;Parent=MS.gene062717.t1
chr1.4 CDS 2540883 2541340 2540883 ID=cds.MS.gene062717.t1;Parent=MS.gene062717.t1
chr1.4 exon 2540506 2540636 2540506 ID=MS.gene062717.t1.exon2;Parent=MS.gene062717.t1
chr1.4 CDS 2540506 2540636 2540506 ID=cds.MS.gene062717.t1;Parent=MS.gene062717.t1
chr1.4 exon 2539309 2539757 2539309 ID=MS.gene062717.t1.exon3;Parent=MS.gene062717.t1
chr1.4 CDS 2539309 2539757 2539309 ID=cds.MS.gene062717.t1;Parent=MS.gene062717.t1
Gene Sequence

>MS.gene062717

ATGTGTATAGTATATCTTTCGCCGCTTGCAGTTGTAGGATTGCGGCTTATCAGTATTCGTTATAGTAGGAAGGCAGTATCATTCTTCTTTGGACTTTGGTTATCTATGTGGCCCTTTCTATTCGAAAAGATAAACAAAACTAAAGTTGTCTTTTCTGGGGATAGTATTCCGATGAGGGAACGTGTGTTACTTATCGCCAATCACAGAACTGAGGTTGATTGGATGTACTTGTGGGATCTTGCTCTTAGAAAGGGAAGACTAGGATTTATAAAATATATCCTTAAGAGCAGCTTAATGAAACTACCGATTTTTGGTTGGGGATTTCAGATTCTGGAATTCATTGCAGTGGAGAGGAAATGGGAGATAGATGAGCAAATATTGCAACAAAATCTTTCAACATTTCAGGATCCGAAAGATCCTTTATGGCTATCTCTTTTTCCCGAAGGAACCGATTATACGTACTACCTCGAATCCTTCTCAGCTCGGATCTCATCTTTTCATTTCTAAGTGTTTTTATTATCATTGATAATGTATTAAATTCATTTTAATTCCACAAATTATTTTATTTGACATTTTGTTTGTATCCATTATGAGCAATATTTTGACTAGCTTCCCAAAATACGCGCATCGTGTTATACCGACAAATAGACAATATATTATTCACTTCAGTGGCACATTTGACATATTATTTGCTTTTTATGCAGTGAACAGAAGTGTAAAAGTAGTCAAAAATTTGCTGCTGAAGTTGGACTTCCTGTGCTGACAAATGTATTACTTCCAAAAACAAAGGGGTTTCACACTTGCTTGGAAACTCTACAAGGCTCATTGGATGCAGGTTTGTTTTATAATTCCTTTTAATTTTTTTAATATTTGATACTCGGTCAATTCATAATGACTTTATGGTAGATATTTATGAAGTTATGCAATCTCTGTTACCAAAATGCAATGGACTTGGCTTTTGAAAATTATGATAAATATGATTGAATTTCAGTTCCAAACGTATGTTCGGATCCAAACGTATGTTTGGATCCAAATATATCTTTGCAGTTTCTCAATTGTAAACAAGCATACAAGTACTTCACTTTTTCTCCTTGCTTTGAATTTACCCTGTAGGATGCTCTGTGTTTTTTGCTTTAGGGGTGGATTTCTTTAGGTGTACTTATAGCTTACATTATGTGATCGAAATCGGCAGTAAGAAATTCCACGCATTCAGCACGCCGGTCGTTGTTCGATCAAAATCGGAAGGTTCAAATTTCAAGCTCGGCATTTTAATTTGAAAATAAAAAAAAATCAAAATCTGCATTCAAACCTGGACCGTTTGATCTCGATCCAACGACCACCAATATGCTAACTGGATGCATGTAATTACTTTCTGCAGTCAATCCGAATTTGTGTATATTCCATATCATTCCCTAGGGAAGCAGAATGCTAGTTCACAAAACCAAGTTTGCTTTCACTAGGTGTTCAACAATATTTTGACATGGCAGTTTACATAATGTTCCAACTTTCCCCTTGTCTCTTTTCTTTTCTAAATTTGTTCAACGTTTAAAGTATGTTAAACTTTATCTCTCAATCACGTGCAGTATATGACGTGACAATTGCATACAAGAATCAATGTCCTTCCTTTCTAGACAATGTTTTTGGTCTTGATCCTTCAGAAGTCCACTTGCATATTCGGCGTATTCCTGTAGAGGAGATTCCAATTTCTGAAACTAAAGCTGCTTCTTGGCTAATGGATGCATTCCAAATCAAGGACCAATTGCTTTCTGATTTCAAGGTTCAAGGCCATTTCCCTAACCAACAAAATGAAGTGGAATTCTCGACATTCAAGTGCCTATTCACTTTTACCGTGATAGTTTGTTTTACCGCCTTGTTTACTTATTTTACCTTGTTTTCTCACATCGGGTTCAAGTTATATGTAGGTCTATCTTGTGCATATCTTTCTATTGCAACGCGCTACAAAATTCAATTGATGCCTTTAACAGATTATGTACATGCATTCTATAACAGCAAGAAACAAAAGAATGCATAG
Protein sequence

>MS.gene062717.t1

MCIVYLSPLAVVGLRLISIRYSRKAVSFFFGLWLSMWPFLFEKINKTKVVFSGDSIPMRERVLLIANHRTEVDWMYLWDLALRKGRLGFIKYILKSSLMKLPIFGWGFQILEFIAVERKWEIDEQILQQNLSTFQDPKDPLWLSLFPEGTDYTEQKCKSSQKFAAEVGLPVLTNVLLPKTKGFHTCLETLQGSLDAVYDVTIAYKNQCPSFLDNVFGLDPSEVHLHIRRIPVEEIPISETKAASWLMDAFQIKDQLLSDFKVQGHFPNQQNEVEFSTFKCLFTFTVIVCFTALFTYFTLFSHIGFKLYVGLSCAYLSIATRYKIQLMPLTDYVHAFYNSKKQKNA