AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062729

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062729.t1 MTR_1g021740 95.688 487 21 0 1 487 1 487 0.0 932
MS.gene062729.t1 MTR_8g069470 55.654 451 200 0 36 486 51 501 0.0 522
MS.gene062729.t1 MTR_5g033100 52.708 480 227 0 2 481 23 502 1.42e-177 508
MS.gene062729.t1 MTR_1g100180 49.388 490 239 3 1 482 1 489 5.68e-169 486
MS.gene062729.t1 MTR_2g088400 48.485 495 239 4 2 482 8 500 6.63e-156 453
MS.gene062729.t1 MTR_3g099700 50.976 461 225 1 20 479 31 491 3.65e-155 451
MS.gene062729.t1 MTR_5g032720 48.246 456 235 1 28 482 29 484 1.49e-154 449
MS.gene062729.t1 MTR_2g022980 47.639 487 246 3 2 479 7 493 7.35e-152 442
MS.gene062729.t1 MTR_4g048000 46.918 503 245 5 2 482 10 512 1.14e-150 440
MS.gene062729.t1 MTR_7g087410 47.020 453 240 0 27 479 23 475 1.09e-147 431
MS.gene062729.t1 MTR_7g087400 45.299 468 250 3 13 480 13 474 1.03e-145 426
MS.gene062729.t1 MTR_5g032750 47.689 476 239 3 6 480 16 482 1.85e-145 426
MS.gene062729.t1 MTR_3g099720 44.325 467 260 0 12 478 18 484 2.76e-142 417
MS.gene062729.t1 MTR_2g088400 48.230 452 218 4 2 439 8 457 1.62e-139 409
MS.gene062729.t1 MTR_6g027200 45.217 460 248 3 22 480 25 481 2.50e-136 402
MS.gene062729.t1 MTR_6g027190 46.781 466 246 2 16 480 5 469 3.76e-136 402
MS.gene062729.t1 MTR_6g047610 41.875 480 275 3 3 482 1 476 1.09e-131 390
MS.gene062729.t1 MTR_7g087410 48.705 386 198 0 94 479 1 386 9.45e-131 385
MS.gene062729.t1 MTR_7g087370 44.009 459 225 3 21 479 18 444 1.05e-128 382
MS.gene062729.t1 MTR_6g047640 41.667 480 277 2 3 482 1 477 1.32e-128 382
MS.gene062729.t1 MTR_6g047590 42.469 478 269 4 3 479 1 473 1.84e-125 374
MS.gene062729.t1 MTR_5g090290 45.322 481 253 3 8 478 10 490 1.92e-125 377
MS.gene062729.t1 MTR_7g087430 46.373 386 207 0 94 479 1 386 2.50e-124 369
MS.gene062729.t1 MTR_6g047640 39.583 480 268 3 3 482 1 458 3.91e-121 362
MS.gene062729.t1 MTR_8g063590 43.584 452 253 2 36 485 54 505 2.77e-114 347
MS.gene062729.t1 MTR_1g108810 38.655 476 278 6 3 470 1 470 1.25e-103 318
MS.gene062729.t1 MTR_6g081780 39.286 476 271 7 11 476 2 469 6.78e-100 308
MS.gene062729.t1 MTR_6g081410 37.553 474 286 5 11 478 2 471 9.46e-100 308
MS.gene062729.t1 MTR_6g066380 38.429 471 284 4 11 476 2 471 5.51e-97 301
MS.gene062729.t1 MTR_5g087930 43.500 400 204 4 61 459 52 430 1.30e-96 300
MS.gene062729.t1 MTR_6g081300 37.969 453 274 3 36 484 11 460 8.09e-92 287
MS.gene062729.t1 MTR_7g082810 36.878 442 278 1 36 476 21 462 1.49e-90 284
MS.gene062729.t1 MTR_6g081390 38.702 447 265 5 37 478 15 457 1.95e-89 281
MS.gene062729.t1 MTR_6g081810 35.924 476 272 5 1 476 1 443 2.84e-89 280
MS.gene062729.t1 MTR_1g108840 36.895 496 291 10 3 487 8 492 1.04e-87 278
MS.gene062729.t1 MTR_8g106670 37.104 442 276 2 38 478 16 456 9.59e-86 272
MS.gene062729.t1 MTR_6g081240 38.085 449 266 8 27 468 1 444 2.68e-85 270
MS.gene062729.t1 MTR_6g081400 36.689 447 274 5 37 478 18 460 4.29e-84 267
MS.gene062729.t1 MTR_6g004220 35.033 451 291 2 36 484 24 474 8.44e-82 262
MS.gene062729.t1 MTR_6g081310 37.500 448 261 6 53 484 14 458 1.67e-81 261
MS.gene062729.t1 MTR_1g108990 36.712 444 276 3 36 476 26 467 2.43e-78 253
MS.gene062729.t1 MTR_6g004220 35.172 435 280 2 36 468 31 465 4.54e-78 253
MS.gene062729.t1 MTR_2g078930 32.762 467 310 3 4 467 11 476 7.67e-76 246
MS.gene062729.t1 MTR_1g109060 34.842 442 287 1 36 476 25 466 1.44e-74 243
MS.gene062729.t1 MTR_2g078960 33.405 467 308 3 3 467 1 466 2.10e-74 243
MS.gene062729.t1 MTR_5g090280 37.046 413 225 4 94 478 1 406 7.80e-73 236
MS.gene062729.t1 MTR_7g082800 34.715 386 251 1 94 478 1 386 6.21e-72 233
MS.gene062729.t1 MTR_1g109060 34.793 434 282 1 36 468 25 458 6.30e-72 236
MS.gene062729.t1 MTR_2g078970 34.411 433 283 1 36 467 32 464 1.95e-71 235
MS.gene062729.t1 MTR_2g079020 32.719 434 289 2 36 467 38 470 2.04e-71 235
MS.gene062729.t1 MTR_5g010830 30.717 446 296 5 36 474 66 505 6.88e-62 211
MS.gene062729.t1 MTR_0039s0080 28.630 489 333 5 1 481 1 481 2.64e-59 204
MS.gene062729.t1 MTR_1g101500 30.365 438 296 3 42 474 71 504 3.10e-58 201
MS.gene062729.t1 MTR_3g111020 27.754 472 332 4 12 477 26 494 1.50e-57 200
MS.gene062729.t1 MTR_5g067460 27.902 448 312 4 36 477 56 498 6.60e-57 198
MS.gene062729.t1 MTR_6g004220 32.153 367 247 2 36 400 31 397 1.42e-50 179
MS.gene062729.t1 MTR_6g088805 26.452 465 333 3 10 473 7 463 1.49e-49 177
MS.gene062729.t1 MTR_6g043810 27.938 451 311 5 36 474 24 472 1.43e-47 172
MS.gene062729.t1 MTR_6g043810 27.938 451 311 5 36 474 34 482 1.73e-47 172
MS.gene062729.t1 MTR_2g078990 34.364 291 190 1 178 467 3 293 2.43e-46 164
MS.gene062729.t1 MTR_5g011540 28.702 439 301 6 42 473 50 483 5.64e-44 162
MS.gene062729.t1 MTR_8g102430 26.424 439 302 3 36 473 24 442 2.24e-43 159
MS.gene062729.t1 MTR_4g027920 26.096 456 322 5 26 472 147 596 2.19e-41 156
MS.gene062729.t1 MTR_6g004220 32.374 278 186 2 36 311 31 308 1.92e-37 140
MS.gene062729.t1 MTR_2g435930 22.899 476 350 3 18 476 2 477 9.48e-34 133
MS.gene062729.t1 MTR_2g079030 36.410 195 124 0 273 467 319 513 5.54e-32 129
MS.gene062729.t1 MTR_4g131290 52.459 61 29 0 107 167 13 73 2.39e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062729.t1 AT1G47530 66.807 476 155 2 6 481 8 480 0.0 647
MS.gene062729.t1 AT1G47530 70.619 388 114 0 94 481 1 388 0.0 565
MS.gene062729.t1 AT3G26590 53.626 455 211 0 30 484 39 493 7.48e-180 513
MS.gene062729.t1 AT1G23300 54.487 468 207 1 15 482 29 490 1.26e-178 511
MS.gene062729.t1 AT5G38030 51.760 483 227 2 8 484 11 493 4.63e-177 506
MS.gene062729.t1 AT1G12950 52.220 473 220 1 9 481 46 512 2.81e-174 500
MS.gene062729.t1 AT4G00350 54.933 446 200 1 35 480 87 531 1.09e-170 492
MS.gene062729.t1 AT4G25640 50.924 487 226 4 1 482 1 479 1.72e-157 456
MS.gene062729.t1 AT4G25640 50.924 487 226 4 1 482 1 479 7.58e-157 456
MS.gene062729.t1 AT3G26590 53.453 391 182 0 94 484 1 391 1.42e-150 435
MS.gene062729.t1 AT3G21690 49.567 462 230 2 24 482 38 499 2.40e-150 439
MS.gene062729.t1 AT5G65380 48.069 466 241 1 20 484 21 486 5.84e-147 429
MS.gene062729.t1 AT1G61890 47.289 461 240 2 22 479 31 491 2.99e-145 426
MS.gene062729.t1 AT4G25640 51.131 442 203 4 1 437 1 434 5.18e-143 419
MS.gene062729.t1 AT1G11670 47.758 446 232 1 35 479 49 494 1.06e-142 419
MS.gene062729.t1 AT1G33100 45.969 459 248 0 21 479 18 476 1.30e-141 416
MS.gene062729.t1 AT5G44050 48.366 459 234 3 23 479 27 484 1.83e-141 416
MS.gene062729.t1 AT1G33110 45.852 458 248 0 22 479 22 479 3.48e-141 415
MS.gene062729.t1 AT1G61890 47.345 452 235 2 22 470 31 482 6.18e-141 414
MS.gene062729.t1 AT4G21910 44.399 491 262 5 2 482 12 501 5.48e-137 405
MS.gene062729.t1 AT4G21910 44.399 491 262 5 2 482 12 501 5.48e-137 405
MS.gene062729.t1 AT5G10420 47.845 464 235 5 20 479 22 482 6.50e-137 404
MS.gene062729.t1 AT1G33090 45.415 458 250 0 22 479 22 479 3.41e-136 402
MS.gene062729.t1 AT1G33080 44.760 458 253 0 22 479 22 479 1.70e-135 400
MS.gene062729.t1 AT4G21910 44.813 482 255 5 11 482 23 503 1.78e-135 401
MS.gene062729.t1 AT4G21910 44.813 482 255 5 11 482 23 503 1.78e-135 401
MS.gene062729.t1 AT3G59030 47.178 443 233 1 36 477 51 493 9.41e-134 396
MS.gene062729.t1 AT1G33080 44.912 452 249 0 22 473 22 473 9.69e-133 393
MS.gene062729.t1 AT4G21903 43.117 494 273 4 1 487 12 504 1.50e-131 390
MS.gene062729.t1 AT4G21903 43.299 485 267 4 1 478 12 495 2.11e-127 380
MS.gene062729.t1 AT1G61890 46.556 421 222 2 22 439 31 451 2.24e-126 376
MS.gene062729.t1 AT4G21903 46.076 395 212 1 94 487 1 395 1.60e-124 369
MS.gene062729.t1 AT3G03620 39.232 469 285 0 19 487 20 488 1.73e-115 349
MS.gene062729.t1 AT3G03620 39.232 469 285 0 19 487 20 488 1.73e-115 349
MS.gene062729.t1 AT5G17700 39.121 478 284 1 2 479 7 477 2.66e-115 349
MS.gene062729.t1 AT5G17700 39.121 478 284 1 2 479 7 477 2.66e-115 349
MS.gene062729.t1 AT1G33110 46.073 382 206 0 22 403 22 403 1.46e-111 336
MS.gene062729.t1 AT3G59030 45.902 366 197 1 36 400 51 416 5.79e-101 309
MS.gene062729.t1 AT5G52450 41.403 442 257 2 38 478 29 469 2.50e-98 305
MS.gene062729.t1 AT1G73700 40.043 467 269 6 14 476 6 465 1.96e-94 294
MS.gene062729.t1 AT2G34360 39.394 462 262 8 22 478 16 464 2.66e-90 284
MS.gene062729.t1 AT1G71140 36.996 446 272 3 36 476 26 467 2.13e-86 274
MS.gene062729.t1 AT5G17700 38.120 383 237 0 21 403 19 401 2.86e-85 268
MS.gene062729.t1 AT1G66760 32.292 480 306 6 2 476 4 469 2.89e-82 263
MS.gene062729.t1 AT3G23560 33.974 468 302 5 4 467 7 471 7.48e-82 262
MS.gene062729.t1 AT1G15160 36.807 451 266 8 36 476 31 472 3.70e-80 258
MS.gene062729.t1 AT1G15150 35.506 445 280 4 36 476 31 472 4.67e-80 257
MS.gene062729.t1 AT1G66760 32.203 472 301 6 2 468 4 461 7.50e-80 256
MS.gene062729.t1 AT1G64820 34.004 447 280 5 38 476 31 470 3.71e-79 255
MS.gene062729.t1 AT2G04040 32.444 487 313 5 3 487 1 473 5.16e-79 254
MS.gene062729.t1 AT1G66780 34.660 427 274 2 53 476 52 476 6.83e-79 254
MS.gene062729.t1 AT2G34360 36.304 460 235 6 22 477 16 421 1.71e-78 252
MS.gene062729.t1 AT2G04080 31.729 457 305 2 32 487 23 473 2.66e-78 253
MS.gene062729.t1 AT1G15170 35.714 448 277 7 35 476 33 475 1.67e-76 248
MS.gene062729.t1 AT3G23550 33.909 463 295 6 14 467 3 463 1.51e-75 245
MS.gene062729.t1 AT2G04080 31.507 438 299 1 32 468 23 460 2.68e-74 242
MS.gene062729.t1 AT2G04070 32.143 476 313 3 16 487 4 473 4.66e-74 241
MS.gene062729.t1 AT2G04050 32.043 465 312 2 16 476 4 468 1.23e-72 238
MS.gene062729.t1 AT1G15180 35.556 450 273 7 36 476 35 476 1.21e-70 233
MS.gene062729.t1 AT2G04100 32.127 442 299 1 36 476 30 471 3.10e-70 231
MS.gene062729.t1 AT2G04090 31.222 442 303 1 36 476 30 471 4.99e-69 228
MS.gene062729.t1 AT2G04100 31.881 436 296 1 36 470 30 465 8.71e-68 225
MS.gene062729.t1 AT1G15150 36.499 337 211 2 142 476 21 356 2.20e-66 218
MS.gene062729.t1 AT1G15170 36.982 338 208 4 142 476 21 356 7.73e-65 214
MS.gene062729.t1 AT1G58340 29.018 448 317 1 36 482 58 505 1.26e-60 207
MS.gene062729.t1 AT3G23560 32.170 401 265 5 4 400 7 404 6.20e-59 200
MS.gene062729.t1 AT1G66760 32.628 331 216 3 142 468 6 333 1.59e-58 197
MS.gene062729.t1 AT5G19700 27.632 456 326 2 22 473 19 474 5.03e-56 195
MS.gene062729.t1 AT1G66760 29.455 404 266 6 2 400 4 393 8.71e-53 183
MS.gene062729.t1 AT4G29140 26.754 456 333 1 20 474 40 495 9.02e-52 184
MS.gene062729.t1 AT2G38510 25.702 463 332 5 32 487 2 459 2.07e-46 168
MS.gene062729.t1 AT1G71870 25.103 486 336 6 8 472 1 479 4.26e-46 168
MS.gene062729.t1 AT4G23030 29.764 467 315 6 20 478 15 476 3.33e-45 165
MS.gene062729.t1 AT5G52050 26.608 451 312 2 36 473 35 479 6.70e-44 162
MS.gene062729.t1 AT1G15180 35.094 265 161 4 142 400 21 280 2.85e-39 147
MS.gene062729.t1 AT5G49130 24.066 482 340 5 21 478 4 483 3.63e-39 149
MS.gene062729.t1 AT4G22790 25.828 453 319 8 29 473 27 470 3.36e-36 140
MS.gene062729.t1 AT2G04066 38.843 121 74 0 356 476 43 163 1.77e-23 97.4

Find 119 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAAATTGTTCACAAAAT+TGG 0.100214 1.4:+2666437 MS.gene062729:CDS
TATTGTTACCTTTCTATATT+TGG 0.101958 1.4:+2667016 MS.gene062729:CDS
TTCTAATATTGAAACTTAAT+TGG 0.102187 1.4:+2667340 MS.gene062729:CDS
GATTTGTTATTCAGGGTATT+TGG 0.245268 1.4:+2670328 MS.gene062729:intron
TTGGGCTTCACATTTGGTTT+TGG 0.259862 1.4:+2670096 MS.gene062729:CDS
TATAGAAAGGTAACAATAAA+AGG 0.269573 1.4:-2667011 None:intergenic
TCTAATATTGAAACTTAATT+GGG 0.293407 1.4:+2667341 MS.gene062729:CDS
CATTGGATGCAGTATCTATT+TGG 0.295074 1.4:+2667693 MS.gene062729:CDS
TTAATGAAGTCACAATTATA+AGG 0.311749 1.4:-2670389 None:intergenic
ATTGAAAACTCTGTTATTGC+TGG 0.315535 1.4:+2666590 MS.gene062729:CDS
GTTTGCAGTATGAACATAAA+TGG 0.317949 1.4:+2668237 MS.gene062729:intron
ATGATATTGGTGGTGATAAC+TGG 0.319302 1.4:+2667647 MS.gene062729:CDS
ATTGTTACCTTTCTATATTT+GGG 0.330112 1.4:+2667017 MS.gene062729:CDS
CTTGTAGCTTACATAAATCT+TGG 0.340357 1.4:+2670036 MS.gene062729:CDS
ACTCCAAGCACCGTCTGATT+TGG 0.342128 1.4:-2667442 None:intergenic
GTGATGGCAGCTTGGAGTTT+TGG 0.345028 1.4:+2667618 MS.gene062729:intron
CAGAAAGCCGTGTGAGGAAG+TGG 0.348907 1.4:+2670578 MS.gene062729:CDS
ATATTGTTGGGCTTCACATT+TGG 0.357452 1.4:+2670090 MS.gene062729:CDS
CTTGGATCCTACTATGTTGT+TGG 0.361711 1.4:+2670054 MS.gene062729:CDS
TTACCAGCTGGGATATTGTT+GGG 0.365810 1.4:+2670078 MS.gene062729:CDS
GATAAAGCAGTTAAGATTGT+TGG 0.366572 1.4:-2666494 None:intergenic
TAGCTTCAGCTGTTATGTTA+TGG 0.378213 1.4:+2667517 MS.gene062729:CDS
TAGGGTTTAATGTAGCAATA+AGG 0.379904 1.4:+2668280 MS.gene062729:CDS
AAACTTAATTGGGGCTTAGT+TGG 0.382505 1.4:+2667351 MS.gene062729:CDS
GGAAAGTCCTAGTGATGTTA+TGG 0.383348 1.4:+2667274 MS.gene062729:CDS
TGGTTGGCAATAGTTGGGTA+TGG 0.389724 1.4:+2666885 MS.gene062729:intron
TTGTGACTTCATTAACCAAC+TGG 0.390272 1.4:+2670397 MS.gene062729:CDS
TGGATTATCTTGGTGGCTAC+TGG 0.392460 1.4:+2666988 MS.gene062729:CDS
GTTATTCAGGGTATTTGGTC+AGG 0.396483 1.4:+2670333 MS.gene062729:intron
GGTATTTGGTCAGGTCTGAT+TGG 0.397825 1.4:+2670342 MS.gene062729:CDS
ATTACCAGCTGGGATATTGT+TGG 0.407188 1.4:+2670077 MS.gene062729:CDS
TGGGATATGATGATCGCGTT+AGG 0.410431 1.4:+2668261 MS.gene062729:CDS
GCATTCACTTCTACGTTTGT+TGG 0.412634 1.4:+2666533 MS.gene062729:CDS
ACTCCCCTCTTGAAGCTTGT+TGG 0.415152 1.4:+2667042 MS.gene062729:CDS
AAAGCATATGCAAATATTTG+TGG 0.422937 1.4:-2667216 None:intergenic
CATTTGGTGCTGGACATATA+AGG 0.431874 1.4:+2666938 MS.gene062729:CDS
ACACTATGTGGGCAAGCATT+TGG 0.433648 1.4:+2666922 MS.gene062729:CDS
GGTGGTGATAACTGGGCATT+TGG 0.437810 1.4:+2667655 MS.gene062729:CDS
AGCCTTCAACCAGTCTTGTC+TGG 0.438348 1.4:+2669884 MS.gene062729:CDS
TTAGGTGTTGCTATTGGAGC+TGG 0.443556 1.4:+2670003 MS.gene062729:intron
TGTTACATGTACCTCTTAGT+TGG 0.444285 1.4:+2667316 MS.gene062729:CDS
GAATTTACATGCAAAGATCA+TGG 0.447765 1.4:+2666968 MS.gene062729:CDS
TTGTAGGCACGTTTGCTTTG+TGG 0.451174 1.4:+2667187 MS.gene062729:intron
TATGTTGTTGGATTACCAGC+TGG 0.453772 1.4:+2670066 MS.gene062729:CDS
TGCAAAGTTAGCAGTGATAG+TGG 0.456476 1.4:+2669709 MS.gene062729:CDS
GTGATGTTATGGATATCAAT+AGG 0.468621 1.4:+2667285 MS.gene062729:CDS
TAACCAAATCAGACGGTGCT+TGG 0.470170 1.4:+2667439 MS.gene062729:CDS
GCAAAGATCATGGATTATCT+TGG 0.476538 1.4:+2666978 MS.gene062729:CDS
GGCAAACCCTCTAGTGCCAT+TGG 0.477677 1.4:+2667676 MS.gene062729:CDS
TCATGGTGGTTGATAGTCAT+TGG 0.482653 1.4:+2667396 MS.gene062729:CDS
GTTGGGTATGGGAAGTGCAT+TGG 0.484807 1.4:+2666897 MS.gene062729:intron
TTCCTCCCCACTTCCTCACA+CGG 0.485849 1.4:-2670585 None:intergenic
TGGTAATCCAACAACATAGT+AGG 0.486946 1.4:-2670061 None:intergenic
AATATTAGAAGCCAACTAAG+AGG 0.488197 1.4:-2667327 None:intergenic
GGTTGGCAATAGTTGGGTAT+GGG 0.498380 1.4:+2666886 MS.gene062729:intron
CAATTATTCTCAACACATCA+TGG 0.498659 1.4:+2667379 MS.gene062729:CDS
GCTTTATCTCAGTTCTCACT+TGG 0.504778 1.4:+2666509 MS.gene062729:CDS
ATTTGGTCAGGTCTGATTGG+AGG 0.508178 1.4:+2670345 MS.gene062729:CDS
GTGAGAGTATCAAATGAACT+TGG 0.508990 1.4:+2669668 MS.gene062729:CDS
GCAGTATGAACATAAATGGA+TGG 0.518351 1.4:+2668241 MS.gene062729:intron
GTCCACTCTCCTCCTAGTGT+TGG 0.518398 1.4:+2666392 MS.gene062729:CDS
GGGATATGATGATCGCGTTA+GGG 0.520025 1.4:+2668262 MS.gene062729:CDS
AGTTGTTAGTACTATGATCA+TGG 0.530879 1.4:-2669765 None:intergenic
GATGAGGTCGCAGAAGCTGC+TGG 0.531826 1.4:+2667072 MS.gene062729:CDS
TGCTGGACATATAAGGATGA+TGG 0.534475 1.4:+2666945 MS.gene062729:CDS
AACCAGACAAGACTGGTTGA+AGG 0.537386 1.4:-2669886 None:intergenic
ACCAATGGATATTGATGTGA+GGG 0.538819 1.4:-2669735 None:intergenic
TAGCTTGAAGTGAACAATTG+AGG 0.540499 1.4:-2669800 None:intergenic
GAGTGGAGTCGAAGAAGCTA+TGG 0.541517 1.4:+2666459 MS.gene062729:CDS
TGAGAGTATCAAATGAACTT+GGG 0.545113 1.4:+2669669 MS.gene062729:CDS
TCCCTCACATCAATATCCAT+TGG 0.545841 1.4:+2669734 MS.gene062729:CDS
ATTGTTCACTTCAAGCTATG+AGG 0.547129 1.4:+2669805 MS.gene062729:CDS
CAAGATCGAGCTCGTTAACA+TGG 0.548496 1.4:-2666556 None:intergenic
TGTGACTAACCAGACAAGAC+TGG 0.551439 1.4:-2669893 None:intergenic
AAGAAGCTATGGAAAATAGC+TGG 0.552120 1.4:+2666470 MS.gene062729:CDS
GAAAGCCGTGTGAGGAAGTG+GGG 0.552917 1.4:+2670580 MS.gene062729:CDS
AGAAAGCCGTGTGAGGAAGT+GGG 0.557799 1.4:+2670579 MS.gene062729:CDS
AAGATCATGGATTATCTTGG+TGG 0.557828 1.4:+2666981 MS.gene062729:CDS
GGAGTTGCCCAAATATAGAA+AGG 0.559849 1.4:-2667024 None:intergenic
AAGCCCAACAATATCCCAGC+TGG 0.562621 1.4:-2670081 None:intergenic
AATTGTTCACAAAATTGGAG+TGG 0.563939 1.4:+2666442 MS.gene062729:CDS
ATGTTGTTGGATTACCAGCT+GGG 0.564636 1.4:+2670067 MS.gene062729:CDS
CAGTATGAACATAAATGGAT+GGG 0.567017 1.4:+2668242 MS.gene062729:intron
AAATCAGACGGTGCTTGGAG+TGG 0.570545 1.4:+2667444 MS.gene062729:CDS
ACTGCATCCAATGGCACTAG+AGG 0.573790 1.4:-2667683 None:intergenic
CTAATATTGAAACTTAATTG+GGG 0.574308 1.4:+2667342 MS.gene062729:CDS
TGTGGGCAAGCATTTGGTGC+TGG 0.575301 1.4:+2666928 MS.gene062729:CDS
GTATCAAATGAACTTGGGGC+AGG 0.578570 1.4:+2669674 MS.gene062729:CDS
TTGGTACTTGATGATATTGG+TGG 0.583882 1.4:+2667637 MS.gene062729:CDS
AAAACTAAACAAATCTGCAA+AGG 0.586352 1.4:-2667481 None:intergenic
GATCATGGCAACAACACCAA+TGG 0.586370 1.4:-2669750 None:intergenic
GTGCTTGGAGTGGATTCTCA+TGG 0.586711 1.4:+2667454 MS.gene062729:CDS
AGTGCATTGGAGACACTATG+TGG 0.586848 1.4:+2666910 MS.gene062729:CDS
AGTCACAATTATAAGGATTG+TGG 0.587725 1.4:-2670382 None:intergenic
CTGTCCAACAAGCTTCAAGA+GGG 0.591989 1.4:-2667046 None:intergenic
CAAATAGATACTGCATCCAA+TGG 0.601547 1.4:-2667692 None:intergenic
TTCAAGCTATGAGGTTGCGA+AGG 0.603076 1.4:+2669814 MS.gene062729:CDS
TTTGCATACCAAGAAACCAT+AGG 0.608851 1.4:-2666618 None:intergenic
GCTGGACATATAAGGATGAT+GGG 0.608934 1.4:+2666946 MS.gene062729:CDS
TGATATTGGTGGTGATAACT+GGG 0.615582 1.4:+2667648 MS.gene062729:CDS
TTGATATCCATAACATCACT+AGG 0.617645 1.4:-2667281 None:intergenic
TGGACACTGTTATCTAGTAG+AGG 0.620462 1.4:-2666374 None:intergenic
TCCTAGTGTTGGTCATCATG+AGG 0.627707 1.4:+2666403 MS.gene062729:CDS
GAGAGTATCAAATGAACTTG+GGG 0.635268 1.4:+2669670 MS.gene062729:CDS
CTGCATCCAATGGCACTAGA+GGG 0.635362 1.4:-2667682 None:intergenic
TCTGTCCAACAAGCTTCAAG+AGG 0.635759 1.4:-2667047 None:intergenic
CACCAATGGATATTGATGTG+AGG 0.636253 1.4:-2669736 None:intergenic
AAAAGTTCTTACAAGCACAA+AGG 0.640238 1.4:+2667253 MS.gene062729:CDS
GACCAACACTAGGAGGAGAG+TGG 0.645873 1.4:-2666394 None:intergenic
TAGAGGACTGTCCATAGACA+TGG 0.646430 1.4:-2666357 None:intergenic
ATGAAGCAGAAAGCCGTGTG+AGG 0.654673 1.4:+2670572 MS.gene062729:CDS
GTGTTGCTATTGGAGCTGGT+TGG 0.658329 1.4:+2670007 MS.gene062729:CDS
TCATGATGACCAACACTAGG+AGG 0.658415 1.4:-2666401 None:intergenic
GTGCATTGGAGACACTATGT+GGG 0.665609 1.4:+2666911 MS.gene062729:CDS
AGCCGTGTGAGGAAGTGGGG+AGG 0.689480 1.4:+2670583 MS.gene062729:CDS
TCCTCATGATGACCAACACT+AGG 0.692583 1.4:-2666404 None:intergenic
TGTCCAACAAGCTTCAAGAG+GGG 0.717689 1.4:-2667045 None:intergenic
GCTTGTTGGACAGACTGATG+AGG 0.739829 1.4:+2667056 MS.gene062729:CDS
TTATTCTCAACACATCATGG+TGG 0.744685 1.4:+2667382 MS.gene062729:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAATTTCATATCTT+TGG + chr1.4:2670536-2670555 MS.gene062729:intron 10.0%
!! AATATAGTATAATTATTAGA+AGG + chr1.4:2670174-2670193 MS.gene062729:intron 10.0%
!! ATTACAATTAAATAACTTAA+AGG + chr1.4:2669130-2669149 MS.gene062729:intron 10.0%
!! ATTTAACTATAACAATTTAA+GGG + chr1.4:2668949-2668968 MS.gene062729:intron 10.0%
!! GTTATTTAATTGTAATAAAT+TGG - chr1.4:2669126-2669145 None:intergenic 10.0%
!! GTTTATAAATCATAAAATTT+AGG - chr1.4:2666852-2666871 None:intergenic 10.0%
!! TATTTAACTATAACAATTTA+AGG + chr1.4:2668948-2668967 MS.gene062729:intron 10.0%
!!! TATAAACTTGTTTTTATTTT+TGG + chr1.4:2666865-2666884 MS.gene062729:intron 10.0%
!! ATTAAATTCAAGAGAAATAA+GGG - chr1.4:2670141-2670160 None:intergenic 15.0%
!! CTATTGATATAAGTATATTT+GGG + chr1.4:2668583-2668602 MS.gene062729:intron 15.0%
!! GTTACAATTAAATAACTTAA+AGG - chr1.4:2669030-2669011 None:intergenic 15.0%
!! TAGTTTATACGAAAATAATT+TGG + chr1.4:2669256-2669275 MS.gene062729:intron 15.0%
!! TATTAAATTCAAGAGAAATA+AGG - chr1.4:2670142-2670161 None:intergenic 15.0%
!! TCTAATATTGAAACTTAATT+GGG + chr1.4:2667341-2667360 MS.gene062729:CDS 15.0%
!! TCTATTGATATAAGTATATT+TGG + chr1.4:2668582-2668601 MS.gene062729:intron 15.0%
!! TTATTTATTTGAAGAACAAA+AGG - chr1.4:2670503-2670522 None:intergenic 15.0%
!! TTCTAATATTGAAACTTAAT+TGG + chr1.4:2667340-2667359 MS.gene062729:CDS 15.0%
!! TTCTTCAAATAAATAACTTA+TGG + chr1.4:2670507-2670526 MS.gene062729:intron 15.0%
!!! AAACTATTTTCAATTTAACT+TGG + chr1.4:2667551-2667570 MS.gene062729:intron 15.0%
!!! AAGCTATCAAATTTTTATAT+AGG + chr1.4:2667800-2667819 MS.gene062729:intron 15.0%
!!! ATAGAATATTTTGTCATAAT+TGG - chr1.4:2669566-2669585 None:intergenic 15.0%
!!! ATAGTTGATCTTTTTTTTTT+TGG - chr1.4:2668195-2668214 None:intergenic 15.0%
!!! ATTTCAATTTATCTTTTTGT+AGG + chr1.4:2667171-2667190 MS.gene062729:intron 15.0%
!!! TAAGAAAAGATTCTTTTTTT+AGG + chr1.4:2669362-2669381 MS.gene062729:intron 15.0%
!!! TGTAATAAATTGGTTTTTTA+AGG - chr1.4:2669116-2669135 None:intergenic 15.0%
!! AAATCAGTTTATAAACTTAG+AGG + chr1.4:2667839-2667858 MS.gene062729:intron 20.0%
!! AGTGTAATGTACTAATAAAA+TGG - chr1.4:2666656-2666675 None:intergenic 20.0%
!! ATTGTTACCTTTCTATATTT+GGG + chr1.4:2667017-2667036 MS.gene062729:CDS 20.0%
!! CTAATATTGAAACTTAATTG+GGG + chr1.4:2667342-2667361 MS.gene062729:CDS 20.0%
!! CTTAAAATTGTTCACAAAAT+TGG + chr1.4:2666437-2666456 MS.gene062729:CDS 20.0%
!! GTGTTAAGAAAAATACTTAA+AGG + chr1.4:2669039-2669058 MS.gene062729:intron 20.0%
!! TACAGTTATTGTTTAACATT+TGG + chr1.4:2669492-2669511 MS.gene062729:intron 20.0%
!! TATAGAAAGGTAACAATAAA+AGG - chr1.4:2667014-2667033 None:intergenic 20.0%
!! TATTGTTACCTTTCTATATT+TGG + chr1.4:2667016-2667035 MS.gene062729:CDS 20.0%
!! TGACATAATATTCAATTTAG+TGG + chr1.4:2667769-2667788 MS.gene062729:intron 20.0%
!! TTAAATTCAAGAGAAATAAG+GGG - chr1.4:2670140-2670159 None:intergenic 20.0%
!! TTAATGAAGTCACAATTATA+AGG - chr1.4:2670392-2670411 None:intergenic 20.0%
!! TTTCTACTAACTAGAATTAT+AGG + chr1.4:2667127-2667146 MS.gene062729:intron 20.0%
!!! AAAATTTGATAGCTTTAACA+TGG - chr1.4:2667795-2667814 None:intergenic 20.0%
!!! AAACTATTTTTGAGAACTTT+TGG - chr1.4:2668662-2668681 None:intergenic 20.0%
!!! AACTTGTTTTTATTTTTGGT+TGG + chr1.4:2666869-2666888 MS.gene062729:intron 20.0%
!!! ACTAGTTTTATAAATGAGTT+AGG - chr1.4:2667963-2667982 None:intergenic 20.0%
!!! ATATTGATTGTTTGTTAATG+AGG + chr1.4:2670214-2670233 MS.gene062729:intron 20.0%
!!! ATTTTGTGAACAATTTTAAG+TGG - chr1.4:2666437-2666456 None:intergenic 20.0%
!!! CTAGTTTTATAAATGAGTTA+GGG - chr1.4:2667962-2667981 None:intergenic 20.0%
!!! GTACATTTGTTTTTATCATA+AGG + chr1.4:2666818-2666837 MS.gene062729:intron 20.0%
!!! TAAGAAAAGATTCCTTTTTT+AGG - chr1.4:2669451-2669470 None:intergenic 20.0%
!!! TTAACTTGGTTAATATTTGA+TGG + chr1.4:2667565-2667584 MS.gene062729:intron 20.0%
!!! TTTAAAATTGAGCATATGTT+AGG + chr1.4:2668113-2668132 MS.gene062729:intron 20.0%
! AAAACTAAACAAATCTGCAA+AGG - chr1.4:2667484-2667503 None:intergenic 25.0%
! AAAGCATATGCAAATATTTG+TGG - chr1.4:2667219-2667238 None:intergenic 25.0%
! AAGACCTAAACATAATAAAG+AGG - chr1.4:2667996-2668015 None:intergenic 25.0%
! AATAACTTAAAAGATCCCAT+TGG - chr1.4:2668932-2668951 None:intergenic 25.0%
! ATTATACTCCTACCAAATAA+AGG - chr1.4:2668760-2668779 None:intergenic 25.0%
! GTTAAAAATGTTGAATTGTG+TGG + chr1.4:2666697-2666716 MS.gene062729:intron 25.0%
! GTTACAACTAAATGACTTAA+AGG + chr1.4:2669070-2669089 MS.gene062729:intron 25.0%
! TAATTGCTCATAACATATCA+AGG + chr1.4:2668530-2668549 MS.gene062729:intron 25.0%
! TTAACAAATGTTAGTGAGTT+AGG + chr1.4:2670449-2670468 MS.gene062729:intron 25.0%
! TTTATTACCATGCAAGATAA+TGG + chr1.4:2669284-2669303 MS.gene062729:intron 25.0%
!! AAGCAAATAGTTCATTTTGT+AGG - chr1.4:2668407-2668426 None:intergenic 25.0%
!! ATTTTCATAACCAAATCAGA+CGG + chr1.4:2667432-2667451 MS.gene062729:CDS 25.0%
!! TATTTTCAAATGTAGCACTA+CGG + chr1.4:2668019-2668038 MS.gene062729:intron 25.0%
!!! AGATTTTGATATAGCATAAG+TGG - chr1.4:2669596-2669615 None:intergenic 25.0%
!!! AGTGAATTTTGAAAATGTGA+TGG + chr1.4:2667602-2667621 MS.gene062729:intron 25.0%
!!! ATACCTATGATTTGTTATTC+AGG + chr1.4:2670320-2670339 MS.gene062729:intron 25.0%
!!! GAGCAATTAGAAACTTTTTT+AGG - chr1.4:2668519-2668538 None:intergenic 25.0%
!!! GCATTTGTTGTTACTATTTT+AGG + chr1.4:2669985-2670004 MS.gene062729:intron 25.0%
!!! TACCTATGATTTGTTATTCA+GGG + chr1.4:2670321-2670340 MS.gene062729:intron 25.0%
!!! TCTTTTCTTATATTTGAGAC+CGG + chr1.4:2669461-2669480 MS.gene062729:intron 25.0%
!!! TCTTTTCTTATATTTGAGAC+CGG - chr1.4:2669480-2669461 None:intergenic 25.0%
!!! TTAAGTTTTTTCGTACCAAT+GGG + chr1.4:2668914-2668933 MS.gene062729:intron 25.0%
!!! TTTAAGTTTTTTCGTACCAA+TGG + chr1.4:2668913-2668932 MS.gene062729:intron 25.0%
AAAAGTTCTTACAAGCACAA+AGG + chr1.4:2667253-2667272 MS.gene062729:CDS 30.0%
AAACATTCCATTATCTTGCA+TGG - chr1.4:2669294-2669313 None:intergenic 30.0%
AATAACTTAAAGGACCTAAG+TGG - chr1.4:2668874-2668893 None:intergenic 30.0%
AATATTAGAAGCCAACTAAG+AGG - chr1.4:2667330-2667349 None:intergenic 30.0%
AATTGTTCACAAAATTGGAG+TGG + chr1.4:2666442-2666461 MS.gene062729:CDS 30.0%
ACAATGAGAATTTGGAGAAA+GGG - chr1.4:2667736-2667755 None:intergenic 30.0%
AGTCACAATTATAAGGATTG+TGG - chr1.4:2670385-2670404 None:intergenic 30.0%
AGTTGTTAGTACTATGATCA+TGG - chr1.4:2669768-2669787 None:intergenic 30.0%
ATTAACCAACTGGAAAAAAG+AGG + chr1.4:2670407-2670426 MS.gene062729:CDS 30.0%
ATTGAAAACTCTGTTATTGC+TGG + chr1.4:2666590-2666609 MS.gene062729:CDS 30.0%
CAATTATTCTCAACACATCA+TGG + chr1.4:2667379-2667398 MS.gene062729:CDS 30.0%
CAGTATGAACATAAATGGAT+GGG + chr1.4:2668242-2668261 MS.gene062729:intron 30.0%
CTACTCAATGAACCTAAAAA+AGG + chr1.4:2669436-2669455 MS.gene062729:intron 30.0%
CTTGTAGCTTACATAAATCT+TGG + chr1.4:2670036-2670055 MS.gene062729:CDS 30.0%
GAAGCCTCTTTATTATGTTT+AGG + chr1.4:2667989-2668008 MS.gene062729:intron 30.0%
GAATTTACATGCAAAGATCA+TGG + chr1.4:2666968-2666987 MS.gene062729:CDS 30.0%
GATAAAGCAGTTAAGATTGT+TGG - chr1.4:2666497-2666516 None:intergenic 30.0%
GTAGTGCTACATTTGAAAAT+AGG - chr1.4:2668020-2668039 None:intergenic 30.0%
GTGATGTTATGGATATCAAT+AGG + chr1.4:2667285-2667304 MS.gene062729:CDS 30.0%
GTTTGCAGTATGAACATAAA+TGG + chr1.4:2668237-2668256 MS.gene062729:intron 30.0%
TACCCTGAATAACAAATCAT+AGG - chr1.4:2670326-2670345 None:intergenic 30.0%
TAGGGTTTAATGTAGCAATA+AGG + chr1.4:2668280-2668299 MS.gene062729:CDS 30.0%
TATCACAGAACCTCAAAATT+TGG + chr1.4:2668709-2668728 MS.gene062729:intron 30.0%
TCTATGTCACAATGAGAATT+TGG - chr1.4:2667744-2667763 None:intergenic 30.0%
TTATGTTCATACTGCAAACA+TGG - chr1.4:2668236-2668255 None:intergenic 30.0%
TTCATATCTTTGGATTTCAC+AGG + chr1.4:2670546-2670565 MS.gene062729:intron 30.0%
TTGATATCCATAACATCACT+AGG - chr1.4:2667284-2667303 None:intergenic 30.0%
! ACGGGTTTTTCATATAAAAG+TGG + chr1.4:2668038-2668057 MS.gene062729:intron 30.0%
! ATTTTCAAATGTAGCACTAC+GGG + chr1.4:2668020-2668039 MS.gene062729:intron 30.0%
! GCTTGTAAGAACTTTTGAAT+TGG - chr1.4:2667249-2667268 None:intergenic 30.0%
! TGAGTTTTTGCTCCTTTATT+TGG + chr1.4:2668745-2668764 MS.gene062729:intron 30.0%
!! GATTTGTTATTCAGGGTATT+TGG + chr1.4:2670328-2670347 MS.gene062729:intron 30.0%
!! GTTTTGGTACTTGATGATAT+TGG + chr1.4:2667634-2667653 MS.gene062729:CDS 30.0%
!! TGAGAGTATCAAATGAACTT+GGG + chr1.4:2669669-2669688 MS.gene062729:CDS 30.0%
!! TTTTTGCTCCTTTATTTGGT+AGG + chr1.4:2668749-2668768 MS.gene062729:intron 30.0%
!!! ACTATTTTAGGTGTTGCTAT+TGG + chr1.4:2669997-2670016 MS.gene062729:intron 30.0%
!!! ATTTTTGGTTGGCAATAGTT+GGG + chr1.4:2666880-2666899 MS.gene062729:intron 30.0%
!!! TATTTTTGGTTGGCAATAGT+TGG + chr1.4:2666879-2666898 MS.gene062729:intron 30.0%
AAACTTAATTGGGGCTTAGT+TGG + chr1.4:2667351-2667370 MS.gene062729:CDS 35.0%
AAGAAGCTATGGAAAATAGC+TGG + chr1.4:2666470-2666489 MS.gene062729:CDS 35.0%
ACAGAACCTCAAAATTTGGA+GGG + chr1.4:2668713-2668732 MS.gene062729:intron 35.0%
ACCAATGGATATTGATGTGA+GGG - chr1.4:2669738-2669757 None:intergenic 35.0%
AGTTAGGTTCATTAGGAAGT+TGG + chr1.4:2670465-2670484 MS.gene062729:intron 35.0%
ATATTGTTGGGCTTCACATT+TGG + chr1.4:2670090-2670109 MS.gene062729:CDS 35.0%
ATTGTTCACTTCAAGCTATG+AGG + chr1.4:2669805-2669824 MS.gene062729:CDS 35.0%
CAAATAGATACTGCATCCAA+TGG - chr1.4:2667695-2667714 None:intergenic 35.0%
CACAATGAGAATTTGGAGAA+AGG - chr1.4:2667737-2667756 None:intergenic 35.0%
CACGTTAGCATATCAAATGT+CGG + chr1.4:2666737-2666756 MS.gene062729:intron 35.0%
CATTGAGTAGCTAATGTATG+TGG - chr1.4:2669426-2669445 None:intergenic 35.0%
CATTGGATGCAGTATCTATT+TGG + chr1.4:2667693-2667712 MS.gene062729:CDS 35.0%
GCAGTATGAACATAAATGGA+TGG + chr1.4:2668241-2668260 MS.gene062729:intron 35.0%
GTGGTACGAAAACAAACTAA+AGG - chr1.4:2668855-2668874 None:intergenic 35.0%
GTTAGTGAGTTAGGTTCATT+AGG + chr1.4:2670458-2670477 MS.gene062729:intron 35.0%
TAGCTTCAGCTGTTATGTTA+TGG + chr1.4:2667517-2667536 MS.gene062729:CDS 35.0%
TAGCTTGAAGTGAACAATTG+AGG - chr1.4:2669803-2669822 None:intergenic 35.0%
TCATGCAAGTGCATACTATA+AGG + chr1.4:2668160-2668179 MS.gene062729:intron 35.0%
TGAATAGAATATACTCCCTC+CGG + chr1.4:2669330-2669349 MS.gene062729:intron 35.0%
TGGTAATCCAACAACATAGT+AGG - chr1.4:2670064-2670083 None:intergenic 35.0%
TGTTACATGTACCTCTTAGT+TGG + chr1.4:2667316-2667335 MS.gene062729:CDS 35.0%
TTAAGAACAGAGCCGAATAT+GGG + chr1.4:2668680-2668699 MS.gene062729:intron 35.0%
TTATTCTCAACACATCATGG+TGG + chr1.4:2667382-2667401 MS.gene062729:CDS 35.0%
TTGTGACTTCATTAACCAAC+TGG + chr1.4:2670397-2670416 MS.gene062729:CDS 35.0%
TTTAAGAACAGAGCCGAATA+TGG + chr1.4:2668679-2668698 MS.gene062729:intron 35.0%
TTTGCATACCAAGAAACCAT+AGG - chr1.4:2666621-2666640 None:intergenic 35.0%
! AAGATCATGGATTATCTTGG+TGG + chr1.4:2666981-2667000 MS.gene062729:CDS 35.0%
! GCAAAGATCATGGATTATCT+TGG + chr1.4:2666978-2666997 MS.gene062729:CDS 35.0%
! TACATTTTACGTGCGAATTC+AGG + chr1.4:2668356-2668375 MS.gene062729:intron 35.0%
! TTCTTATATTTGAGACCGGA+GGG + chr1.4:2669465-2669484 MS.gene062729:intron 35.0%
! TTCTTATATTTGAGACCGGA+GGG - chr1.4:2669484-2669465 None:intergenic 35.0%
! TTGGTACTTGATGATATTGG+TGG + chr1.4:2667637-2667656 MS.gene062729:CDS 35.0%
! TTTCTTATATTTGAGACCGG+AGG + chr1.4:2669464-2669483 MS.gene062729:intron 35.0%
! TTTCTTATATTTGAGACCGG+AGG - chr1.4:2669483-2669464 None:intergenic 35.0%
! TTTGAACCCTCCAAATTTTG+AGG - chr1.4:2668722-2668741 None:intergenic 35.0%
! TTTGAGGTTCTGTGATATGT+TGG - chr1.4:2668706-2668725 None:intergenic 35.0%
!! AGTTTGTTTTCGTACCACTT+AGG + chr1.4:2668857-2668876 MS.gene062729:intron 35.0%
!! ATGATATTGGTGGTGATAAC+TGG + chr1.4:2667647-2667666 MS.gene062729:CDS 35.0%
!! GAGAGTATCAAATGAACTTG+GGG + chr1.4:2669670-2669689 MS.gene062729:CDS 35.0%
!! GTGAGAGTATCAAATGAACT+TGG + chr1.4:2669668-2669687 MS.gene062729:CDS 35.0%
!! TGATATTGGTGGTGATAACT+GGG + chr1.4:2667648-2667667 MS.gene062729:CDS 35.0%
!!! ACTAACCTCTTTTTTCCAGT+TGG - chr1.4:2670415-2670434 None:intergenic 35.0%
ATGTTGTTGGATTACCAGCT+GGG + chr1.4:2670067-2670086 MS.gene062729:CDS 40.0%
ATTACCAGCTGGGATATTGT+TGG + chr1.4:2670077-2670096 MS.gene062729:CDS 40.0%
CAATAACTGTATACTCCCTC+CGG - chr1.4:2669483-2669502 None:intergenic 40.0%
CACAGAACCTCAAAATTTGG+AGG + chr1.4:2668712-2668731 MS.gene062729:intron 40.0%
CACCAATGGATATTGATGTG+AGG - chr1.4:2669739-2669758 None:intergenic 40.0%
CTTGGATCCTACTATGTTGT+TGG + chr1.4:2670054-2670073 MS.gene062729:CDS 40.0%
GAGTAGTTCGTGTAAGAGAA+GGG + chr1.4:2668484-2668503 MS.gene062729:intron 40.0%
GCATTCACTTCTACGTTTGT+TGG + chr1.4:2666533-2666552 MS.gene062729:CDS 40.0%
GCTGGACATATAAGGATGAT+GGG + chr1.4:2666946-2666965 MS.gene062729:CDS 40.0%
GCTTTATCTCAGTTCTCACT+TGG + chr1.4:2666509-2666528 MS.gene062729:CDS 40.0%
GGAAAGTCCTAGTGATGTTA+TGG + chr1.4:2667274-2667293 MS.gene062729:CDS 40.0%
GGAGTTGCCCAAATATAGAA+AGG - chr1.4:2667027-2667046 None:intergenic 40.0%
GTGATATGTTGGCCCATATT+CGG - chr1.4:2668695-2668714 None:intergenic 40.0%
TATGTTGTTGGATTACCAGC+TGG + chr1.4:2670066-2670085 MS.gene062729:CDS 40.0%
TCCCTCACATCAATATCCAT+TGG + chr1.4:2669734-2669753 MS.gene062729:CDS 40.0%
TGAGTAGTTCGTGTAAGAGA+AGG + chr1.4:2668483-2668502 MS.gene062729:intron 40.0%
TGCAAAGTTAGCAGTGATAG+TGG + chr1.4:2669709-2669728 MS.gene062729:CDS 40.0%
TGCTGGACATATAAGGATGA+TGG + chr1.4:2666945-2666964 MS.gene062729:CDS 40.0%
TGGACACTGTTATCTAGTAG+AGG - chr1.4:2666377-2666396 None:intergenic 40.0%
TTACCAGCTGGGATATTGTT+GGG + chr1.4:2670078-2670097 MS.gene062729:CDS 40.0%
TTGAAAATGTGATGGCAGCT+TGG + chr1.4:2667610-2667629 MS.gene062729:intron 40.0%
TTGAGCATATGTTAGGCTTG+TGG + chr1.4:2668120-2668139 MS.gene062729:intron 40.0%
! GTTATTCAGGGTATTTGGTC+AGG + chr1.4:2670333-2670352 MS.gene062729:intron 40.0%
!! CATTTGGTGCTGGACATATA+AGG + chr1.4:2666938-2666957 MS.gene062729:CDS 40.0%
!! TCATGGTGGTTGATAGTCAT+TGG + chr1.4:2667396-2667415 MS.gene062729:CDS 40.0%
!! TGGTTTTGCCTATGGTTTCT+TGG + chr1.4:2666610-2666629 MS.gene062729:CDS 40.0%
!! TTGGGCTTCACATTTGGTTT+TGG + chr1.4:2670096-2670115 MS.gene062729:CDS 40.0%
!!! GTTATTGCTGGTTTTGCCTA+TGG + chr1.4:2666602-2666621 MS.gene062729:CDS 40.0%
ACACTATGTGGGCAAGCATT+TGG + chr1.4:2666922-2666941 MS.gene062729:CDS 45.0%
AGTGCATTGGAGACACTATG+TGG + chr1.4:2666910-2666929 MS.gene062729:CDS 45.0%
CAAGATCGAGCTCGTTAACA+TGG - chr1.4:2666559-2666578 None:intergenic 45.0%
CGAAGCTTATCCAATCGAGT+GGG - chr1.4:2667905-2667924 None:intergenic 45.0%
CTGTCCAACAAGCTTCAAGA+GGG - chr1.4:2667049-2667068 None:intergenic 45.0%
GATCATGGCAACAACACCAA+TGG - chr1.4:2669753-2669772 None:intergenic 45.0%
GGGATATGATGATCGCGTTA+GGG + chr1.4:2668262-2668281 MS.gene062729:CDS 45.0%
GGTTGGCAATAGTTGGGTAT+GGG + chr1.4:2666886-2666905 MS.gene062729:intron 45.0%
GTATCAAATGAACTTGGGGC+AGG + chr1.4:2669674-2669693 MS.gene062729:CDS 45.0%
GTGCATTGGAGACACTATGT+GGG + chr1.4:2666911-2666930 MS.gene062729:CDS 45.0%
TAACCAAATCAGACGGTGCT+TGG + chr1.4:2667439-2667458 MS.gene062729:CDS 45.0%
TCATGATGACCAACACTAGG+AGG - chr1.4:2666404-2666423 None:intergenic 45.0%
TCCTCATGATGACCAACACT+AGG - chr1.4:2666407-2666426 None:intergenic 45.0%
TCGAAGCTTATCCAATCGAG+TGG - chr1.4:2667906-2667925 None:intergenic 45.0%
TCTGTCCAACAAGCTTCAAG+AGG - chr1.4:2667050-2667069 None:intergenic 45.0%
TGGGATATGATGATCGCGTT+AGG + chr1.4:2668261-2668280 MS.gene062729:CDS 45.0%
TGGTTGGCAATAGTTGGGTA+TGG + chr1.4:2666885-2666904 MS.gene062729:intron 45.0%
TGTCCAACAAGCTTCAAGAG+GGG - chr1.4:2667048-2667067 None:intergenic 45.0%
TGTGACTAACCAGACAAGAC+TGG - chr1.4:2669896-2669915 None:intergenic 45.0%
TTCAAGCTATGAGGTTGCGA+AGG + chr1.4:2669814-2669833 MS.gene062729:CDS 45.0%
TTGTAGGCACGTTTGCTTTG+TGG + chr1.4:2667187-2667206 MS.gene062729:intron 45.0%
TTTCGATACACCCACTCGAT+TGG + chr1.4:2667892-2667911 MS.gene062729:intron 45.0%
! AACCAGACAAGACTGGTTGA+AGG - chr1.4:2669889-2669908 None:intergenic 45.0%
! TGGATTATCTTGGTGGCTAC+TGG + chr1.4:2666988-2667007 MS.gene062729:CDS 45.0%
!! CACAACAATGCAAGAGTGCT+AGG - chr1.4:2668084-2668103 None:intergenic 45.0%
!! TCCTAGTGTTGGTCATCATG+AGG + chr1.4:2666403-2666422 MS.gene062729:CDS 45.0%
!! TTAGGTGTTGCTATTGGAGC+TGG + chr1.4:2670003-2670022 MS.gene062729:intron 45.0%
!!! CACATTTGGTTTTGGAGCAG+AGG + chr1.4:2670104-2670123 MS.gene062729:CDS 45.0%
!! ATTACAATTAAATAATTTAA+AGG - chr1.4:2668824-2668843 None:intergenic 5.0%
!! ATTATTTAATTGTAATAATT+TGG + chr1.4:2668828-2668847 MS.gene062729:intron 5.0%
!!! ATGATATTAAATTTTTATAT+TGG + chr1.4:2667936-2667955 MS.gene062729:intron 5.0%
!!! ATTATGAAATAATTTTTTTA+AGG + chr1.4:2668780-2668799 MS.gene062729:intron 5.0%
AAATCAGACGGTGCTTGGAG+TGG + chr1.4:2667444-2667463 MS.gene062729:CDS 50.0%
AAGCCCAACAATATCCCAGC+TGG - chr1.4:2670084-2670103 None:intergenic 50.0%
ACTCCCCTCTTGAAGCTTGT+TGG + chr1.4:2667042-2667061 MS.gene062729:CDS 50.0%
ACTGCATCCAATGGCACTAG+AGG - chr1.4:2667686-2667705 None:intergenic 50.0%
AGAAAGCCGTGTGAGGAAGT+GGG + chr1.4:2670579-2670598 MS.gene062729:CDS 50.0%
ATGAAGCAGAAAGCCGTGTG+AGG + chr1.4:2670572-2670591 MS.gene062729:CDS 50.0%
CTGCATCCAATGGCACTAGA+GGG - chr1.4:2667685-2667704 None:intergenic 50.0%
GAGTGGAGTCGAAGAAGCTA+TGG + chr1.4:2666459-2666478 MS.gene062729:CDS 50.0%
GGTGGTGATAACTGGGCATT+TGG + chr1.4:2667655-2667674 MS.gene062729:CDS 50.0%
! ACTCCAAGCACCGTCTGATT+TGG - chr1.4:2667445-2667464 None:intergenic 50.0%
! AGCCTTCAACCAGTCTTGTC+TGG + chr1.4:2669884-2669903 MS.gene062729:CDS 50.0%
! GCTTGTTGGACAGACTGATG+AGG + chr1.4:2667056-2667075 MS.gene062729:CDS 50.0%
! GTGATGGCAGCTTGGAGTTT+TGG + chr1.4:2667618-2667637 MS.gene062729:intron 50.0%
! GTGCTTGGAGTGGATTCTCA+TGG + chr1.4:2667454-2667473 MS.gene062729:CDS 50.0%
! GTGTTGCTATTGGAGCTGGT+TGG + chr1.4:2670007-2670026 MS.gene062729:CDS 50.0%
! GTTGGGTATGGGAAGTGCAT+TGG + chr1.4:2666897-2666916 MS.gene062729:intron 50.0%
CAGAAAGCCGTGTGAGGAAG+TGG + chr1.4:2670578-2670597 MS.gene062729:CDS 55.0%
GAAAGCCGTGTGAGGAAGTG+GGG + chr1.4:2670580-2670599 MS.gene062729:CDS 55.0%
GACCAACACTAGGAGGAGAG+TGG - chr1.4:2666397-2666416 None:intergenic 55.0%
GGCAAACCCTCTAGTGCCAT+TGG + chr1.4:2667676-2667695 MS.gene062729:CDS 55.0%
GTCCACTCTCCTCCTAGTGT+TGG + chr1.4:2666392-2666411 MS.gene062729:CDS 55.0%
TTCCTCCCCACTTCCTCACA+CGG - chr1.4:2670588-2670607 None:intergenic 55.0%
!! TGTGGGCAAGCATTTGGTGC+TGG + chr1.4:2666928-2666947 MS.gene062729:CDS 55.0%
GATGAGGTCGCAGAAGCTGC+TGG + chr1.4:2667072-2667091 MS.gene062729:CDS 60.0%
AGCCGTGTGAGGAAGTGGGG+AGG + chr1.4:2670583-2670602 MS.gene062729:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 2666359 2670627 2666359 ID=MS.gene062729
chr1.4 mRNA 2666359 2670627 2666359 ID=MS.gene062729.t1;Parent=MS.gene062729
chr1.4 exon 2666359 2666631 2666359 ID=MS.gene062729.t1.exon1;Parent=MS.gene062729.t1
chr1.4 CDS 2666359 2666631 2666359 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2666898 2667093 2666898 ID=MS.gene062729.t1.exon2;Parent=MS.gene062729.t1
chr1.4 CDS 2666898 2667093 2666898 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2667193 2667538 2667193 ID=MS.gene062729.t1.exon3;Parent=MS.gene062729.t1
chr1.4 CDS 2667193 2667538 2667193 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2667628 2667714 2667628 ID=MS.gene062729.t1.exon4;Parent=MS.gene062729.t1
chr1.4 CDS 2667628 2667714 2667628 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2668245 2668301 2668245 ID=MS.gene062729.t1.exon5;Parent=MS.gene062729.t1
chr1.4 CDS 2668245 2668301 2668245 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2669667 2669905 2669667 ID=MS.gene062729.t1.exon6;Parent=MS.gene062729.t1
chr1.4 CDS 2669667 2669905 2669667 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2670007 2670125 2670007 ID=MS.gene062729.t1.exon7;Parent=MS.gene062729.t1
chr1.4 CDS 2670007 2670125 2670007 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2670342 2670428 2670342 ID=MS.gene062729.t1.exon8;Parent=MS.gene062729.t1
chr1.4 CDS 2670342 2670428 2670342 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
chr1.4 exon 2670568 2670627 2670568 ID=MS.gene062729.t1.exon9;Parent=MS.gene062729.t1
chr1.4 CDS 2670568 2670627 2670568 ID=cds.MS.gene062729.t1;Parent=MS.gene062729.t1
Gene Sequence

>MS.gene062729

ATGTCTATGGACAGTCCTCTACTAGATAACAGTGTCCACTCTCCTCCTAGTGTTGGTCATCATGAGGAAGAGAAGCCACTTAAAATTGTTCACAAAATTGGAGTGGAGTCGAAGAAGCTATGGAAAATAGCTGGTCCAACAATCTTAACTGCTTTATCTCAGTTCTCACTTGGTGCATTCACTTCTACGTTTGTTGGCCATGTTAACGAGCTCGATCTTGCAGCTTTTTCTATTGAAAACTCTGTTATTGCTGGTTTTGCCTATGGTTTCTTGGTATGCAAACTTTTTACGTCTCCATTTTATTAGTACATTACACTTGTTCGACAAAATTCACGCACGTTAAAAATGTTGAATTGTGTGGAATTGCGTGTAAGAACACACGTTAGCATATCAAATGTCGGTGCAGTCTTTTATTATCTATGTTGTACTTACACAACGCATTATACTTATCTTTAACTAGTACATTTGTTTTTATCATAAGGAATTGTATCCTAAATTTTATGATTTATAAACTTGTTTTTATTTTTGGTTGGCAATAGTTGGGTATGGGAAGTGCATTGGAGACACTATGTGGGCAAGCATTTGGTGCTGGACATATAAGGATGATGGGAATTTACATGCAAAGATCATGGATTATCTTGGTGGCTACTGGCCTTTTATTGTTACCTTTCTATATTTGGGCAACTCCCCTCTTGAAGCTTGTTGGACAGACTGATGAGGTCGCAGAAGCTGCTGGTTAGTTACTTGTATATGTTTGTTTAATTAAATTTTCTACTAACTAGAATTATAGGTACATGTTGTATGTATTTATTATTTCAATTTATCTTTTTGTAGGCACGTTTGCTTTGTGGATGATACCACAAATATTTGCATATGCTTTGAACTTTCCAATTCAAAAGTTCTTACAAGCACAAAGGAAAGTCCTAGTGATGTTATGGATATCAATAGGTGTTTTAGTGTTACATGTACCTCTTAGTTGGCTTCTAATATTGAAACTTAATTGGGGCTTAGTTGGAGCTGCAATTATTCTCAACACATCATGGTGGTTGATAGTCATTGGTCAACTTCTGTACATTTTCATAACCAAATCAGACGGTGCTTGGAGTGGATTCTCATGGCTAGCCTTTGCAGATTTGTTTAGTTTTTTGAAGCTTTCTATAGCTTCAGCTGTTATGTTATGGTATATTTTCTCAAACTATTTTCAATTTAACTTGGTTAATATTTGATGGAAATTTGATGTTAAAGTGAATTTTGAAAATGTGATGGCAGCTTGGAGTTTTGGTACTTGATGATATTGGTGGTGATAACTGGGCATTTGGCAAACCCTCTAGTGCCATTGGATGCAGTATCTATTTGGTAAATGTTATTTAGCATCCCTTTCTCCAAATTCTCATTGTGACATAGAAGTATTGACATAATATTCAATTTAGTGGCCATGTTAAAGCTATCAAATTTTTATATAGGAGATGACTCATTTATAAAATCAGTTTATAAACTTAGAGGCATCACTCTTTATAAAAAGAGTTCTGAATTTTTCGATACACCCACTCGATTGGATAAGCTTCGATATCAATTATATGATATTAAATTTTTATATTGGCCCTAACTCATTTATAAAACTAGTTTATAAGAAGCCTCTTTATTATGTTTAGGTCTTGCCTATTTTCAAATGTAGCACTACGGGTTTTTCATATAAAAGTGGTAGACATTTTCTCTTATGTTCCTAGCACTCTTGCATTGTTGTGTGCTTGCAATTTAAAATTGAGCATATGTTAGGCTTGTGGTAGTCATTATTAGCATATCATGCAAGTGCATACTATAAGGTGAGCATGACCAAAAAAAAAAAGATCAACTATAGCTAATGATTTGTATTTCCATGTTTGCAGTATGAACATAAATGGATGGGATATGATGATCGCGTTAGGGTTTAATGTAGCAATAAGGTGATTATCTTTTCTTCTATTTCTTTTCTTAATAAATTTAGCGTGTTTAAAGAGTACATTTTACGTGCGAATTCAGGTTGTGTTTGTCACTTCGACTATGTTCCTACAAAATGAACTATTTGCTTTTGAGAGTAAACTACTTAACTTCAATAGTTGATAGTAAGTGTTAAAAACTGTAAAGTGAGTAGTTCGTGTAAGAGAAGGGAAATATTTTCCTAAAAAAGTTTCTAATTGCTCATAACATATCAAGGATCTATTTAACTTGATTTATTTGAGCTTATCTATTGATATAAGTATATTTGGGAAAATATATGAAAATAATTTATAACATGTTCATAAGTTGTTTTTAACTTATTTCCAAAAGTTCTCAAAAATAGTTTAAGAACAGAGCCGAATATGGGCCAACATATCACAGAACCTCAAAATTTGGAGGGTTCAAATTTTGAGTTTTTGCTCCTTTATTTGGTAGGAGTATAATATTATGAAATAATTTTTTTAAGGTTAAATTACATCATAAATCCTTTAAATTATTTAATTGTAATAATTTGGTCCTTTAGTTTGTTTTCGTACCACTTAGGTCCTTTAAGTTATTTAATTGTAACAAACAATCATTTAAGTTTTTTCGTACCAATGGGATCTTTTAAGTTATTTAACTATAACAATTTAAGGGATTTCAGATTTAAAAATGTAAGTTATTCTGAGCTTAAAAGTTACAATTAAATAACTTAAAGAACTAAGTGTTAAGAAAAATACTTAAAGGACTAACTTGTTACAACTAAATGACTTAAAGGACGTAAGTGATACGAAAAAACCTTAAAAAACCAATTTATTACAATTAAATAACTTAAAGGATGTTCAATGTAATTTAGCATTTTCTTAATTACTAAAGTTACACTCTCTTTTTGAAAAAAATAATATAAGACGATCATATGTATCGAAAGTCGAAACAATTTATAGTTTATACGAAAATAATTTGGCTTTATTTATTACCATGCAAGATAATGGAATGTTTGTGTTTCTCATTCATCTGAATAGAATATACTCCCTCCGGTCTCAAATATAAGAAAAGATTCTTTTTTTAGGTTCATTGAATAATTAATGTATCTGCTTTACAATGTAGACCACATACATTAGCTACTCAATGAACCTAAAAAAGGAATCTTTTCTTATATTTGAGACCGGAGGGAGTATACAGTTATTGTTTAACATTTGGTCAACTATCTAATTATGATACAACATAATTACTGAAAAATAATTATCGCCAATTATGACAAAATATTCTATTCTATCACCACTTATGCTATATCAAAATCTATATCAATTGAAAGCATAAGTTTGTACTGATTATGTTTAATTTATGAACAGTGTGAGAGTATCAAATGAACTTGGGGCAGGTGATTTTCGAGCTGCAAAGTTAGCAGTGATAGTGGTTTCCCTCACATCAATATCCATTGGTGTTGTTGCCATGATCATAGTACTAACAACTAGAGATTACTTTCCTCAATTGTTCACTTCAAGCTATGAGGTTGCGAAGGAAACAACTAAACTTGCTGCTTTGCTTAGTATCACAGTACTTCTAAACAGCCTTCAACCAGTCTTGTCTGGTTAGTCACAAAAAGATTGAAACATTGTTACTATAAATGTTTTTATGCATTGAAACATTGTTACTATAAATTAATTTATGCATTTGTTGTTACTATTTTAGGTGTTGCTATTGGAGCTGGTTGGCAATCTCTTGTAGCTTACATAAATCTTGGATCCTACTATGTTGTTGGATTACCAGCTGGGATATTGTTGGGCTTCACATTTGGTTTTGGAGCAGAGGTGATTTACTACCCCTTATTTCTCTTGAATTTAATACTAAAATACTTGAATATAGTATAATTATTAGAAGGATTCGTTTAGTAATTTAATATTGATTGTTTGTTAATGAGGACTATTTTGTAATTTTCTATTGTATTATCAACATAATTTTTTTTAAAAGACATAAATTTATTTGTTAATTCTTACTTTTTTTTATACCTATGATTTGTTATTCAGGGTATTTGGTCAGGTCTGATTGGAGGCATAGTTGTGCAGACCACAATCCTTATAATTGTGACTTCATTAACCAACTGGAAAAAAGAGGTTAGTGCAATTTTTTTTTATTAACAAATGTTAGTGAGTTAGGTTCATTAGGAAGTTGGACATGACATTTTCCTTTTGTTCTTCAAATAAATAACTTATGGTATGTTAAAAATTAATTTCATATCTTTGGATTTCACAGGCTGATGAAGCAGAAAGCCGTGTGAGGAAGTGGGGAGGAAAAATTTCAATTGAGAATTAA
Protein sequence

>MS.gene062729.t1

MSMDSPLLDNSVHSPPSVGHHEEEKPLKIVHKIGVESKKLWKIAGPTILTALSQFSLGAFTSTFVGHVNELDLAAFSIENSVIAGFAYGFLLGMGSALETLCGQAFGAGHIRMMGIYMQRSWIILVATGLLLLPFYIWATPLLKLVGQTDEVAEAAGTFALWMIPQIFAYALNFPIQKFLQAQRKVLVMLWISIGVLVLHVPLSWLLILKLNWGLVGAAIILNTSWWLIVIGQLLYIFITKSDGAWSGFSWLAFADLFSFLKLSIASAVMLCLEFWYLMILVVITGHLANPLVPLDAVSICMNINGWDMMIALGFNVAISVRVSNELGAGDFRAAKLAVIVVSLTSISIGVVAMIIVLTTRDYFPQLFTSSYEVAKETTKLAALLSITVLLNSLQPVLSGVAIGAGWQSLVAYINLGSYYVVGLPAGILLGFTFGFGAEGIWSGLIGGIVVQTTILIIVTSLTNWKKEADEAESRVRKWGGKISIEN