AlfalfaGEDB | MS.gene063207

M. sativa cultivar XinJiangDaYe / MS.gene063207

KEGG: -
GO: -
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063207.t1	XP_013467094.1	90.8	174	16	0	1	174	16	189	1.00E-61	246.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063207.t1	Q9LW68	89.5	124	13	0	51	174	49	172	1.2e-61	237.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063207.t1	A0A072VGF5	90.8	174	16	0	1	174	16	189	7.4e-62	246.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050563	MS.gene063207	0.801257	9.81E-49	-1.69E-46
MS.gene053383	MS.gene063207	0.871929	4.70E-67	-1.69E-46
MS.gene05456	MS.gene063207	0.816662	4.93E-52	-1.69E-46
MS.gene055359	MS.gene063207	0.809289	2.04E-50	-1.69E-46
MS.gene059006	MS.gene063207	0.813052	3.11E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063207.t1	MTR_1g442860	97.990	199	4	0	1	199	16	214	5.00e-145	402
MS.gene063207.t1	MTR_1g075990	79.787	188	32	2	4	191	13	194	1.25e-91	266
MS.gene063207.t1	MTR_5g072510	80.282	142	28	0	40	181	27	168	1.34e-87	257
MS.gene063207.t1	MTR_4g094428	83.704	135	22	0	49	183	45	179	6.29e-87	256
MS.gene063207.t1	MTR_7g097030	94.776	134	7	0	47	180	61	194	8.84e-85	249
MS.gene063207.t1	MTR_1g069825	96.183	131	5	0	50	180	52	182	3.64e-84	248
MS.gene063207.t1	MTR_3g031830	70.760	171	39	2	22	181	3	173	3.14e-76	228
MS.gene063207.t1	MTR_2g092950	71.724	145	41	0	29	173	2	146	4.50e-75	223
MS.gene063207.t1	MTR_4g079930	74.436	133	34	0	41	173	20	152	1.36e-74	223
MS.gene063207.t1	MTR_2g076400	65.644	163	55	1	19	180	1	163	1.16e-73	220
MS.gene063207.t1	MTR_2g016110	72.727	132	36	0	42	173	27	158	9.53e-72	216
MS.gene063207.t1	MTR_1g080210	78.378	148	32	0	27	174	17	164	4.36e-71	214
MS.gene063207.t1	MTR_7g115700	63.924	158	55	2	25	181	2	158	4.65e-71	213
MS.gene063207.t1	MTR_8g086350	72.800	125	34	0	49	173	30	154	5.26e-58	181
MS.gene063207.t1	MTR_1g114020	78.431	51	11	0	43	93	64	114	5.64e-23	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063207.t1	AT2G31160	84.277	159	25	0	23	181	27	185	6.23e-88	258
MS.gene063207.t1	AT1G07090	77.551	147	28	1	36	182	22	163	1.45e-86	253
MS.gene063207.t1	AT3G23290	95.238	126	6	0	49	174	47	172	5.58e-82	242
MS.gene063207.t1	AT5G58500	80.741	135	25	1	48	181	17	151	8.12e-82	241
MS.gene063207.t1	AT5G28490	80.405	148	23	1	32	179	13	154	1.49e-75	225
MS.gene063207.t1	AT2G42610	77.344	128	29	0	46	173	21	148	1.39e-72	218
MS.gene063207.t1	AT2G42610	77.344	128	29	0	46	173	21	148	1.39e-72	218
MS.gene063207.t1	AT3G04510	75.168	149	37	0	31	179	14	162	4.34e-72	217
MS.gene063207.t1	AT1G78815	62.585	147	55	0	49	195	39	185	6.80e-67	204
MS.gene063207.t1	AT4G18610	64.138	145	51	1	30	173	17	161	3.48e-64	197
MS.gene063207.t1	AT1G16910	57.419	155	61	1	24	178	2	151	3.67e-62	191

Find 47 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCGTTACCTCGACCAATT+TGG	0.269006	1.2:+26827273	MS.gene063207:CDS
GGATTTGGATGTCCATAAAA+TGG	0.296810	1.2:-26827324	None:intergenic
GTTGTTGTTGATGTTGTTGA+TGG	0.314316	1.2:-26827132	None:intergenic
GGACATGGTGCAGGTGGATT+TGG	0.320133	1.2:-26827339	None:intergenic
GACTTTACCTTCGCGAAGTT+AGG	0.323181	1.2:+26827478	MS.gene063207:CDS
GGGTTTGTTATGTTGATCAT+AGG	0.347871	1.2:-26827030	None:intergenic
AAGCCAGAAGCTAATCCTTT+TGG	0.361490	1.2:+26827441	MS.gene063207:CDS
GTGGTGGTCTATGGTTTCTT+AGG	0.368488	1.2:-26827209	None:intergenic
GGTGGTGGTGGTGGCGGCTG+AGG	0.370057	1.2:-26827558	None:intergenic
AGACGAGATTGGAACACATT+TGG	0.384522	1.2:+26827180	MS.gene063207:CDS
ACTTTACCTTCGCGAAGTTA+GGG	0.398859	1.2:+26827479	MS.gene063207:CDS
GCACCAAAAGGATTAGCTTC+TGG	0.418214	1.2:-26827444	None:intergenic
GTTGTAGTTGCATTATTTGA+TGG	0.419514	1.2:-26827579	None:intergenic
GGTTTGTTATGTTGATCATA+GGG	0.430093	1.2:-26827029	None:intergenic
GGTAGTCTTGATGCACTCAT+TGG	0.434097	1.2:+26827384	MS.gene063207:CDS
CTTGTCCTCTTCGCCAAGCG+TGG	0.459831	1.2:+26827361	MS.gene063207:CDS
GGTGGTGGTGGCGGCTGAGG+CGG	0.465046	1.2:-26827555	None:intergenic
AGAATCAAAAGAGACGAGAT+TGG	0.470010	1.2:+26827169	MS.gene063207:CDS
GCATTATTTGATGGTGGTGG+TGG	0.471088	1.2:-26827570	None:intergenic
TTATTTGATGGTGGTGGTGG+TGG	0.487231	1.2:-26827567	None:intergenic
TTGTCCTCTTCGCCAAGCGT+GGG	0.488923	1.2:+26827362	MS.gene063207:CDS
CGAGGTAACGCAAGAATTCA+AGG	0.492274	1.2:-26827263	None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG	0.493036	1.2:-26827218	None:intergenic
GTCATCGTCATCGATGATGA+TGG	0.495677	1.2:-26827051	None:intergenic
GTAGTCTTGATGCACTCATT+GGG	0.507513	1.2:+26827385	MS.gene063207:CDS
TGATGTTGTTGTTGTGGACG+AGG	0.511739	1.2:-26827106	None:intergenic
GTTGCATTATTTGATGGTGG+TGG	0.513644	1.2:-26827573	None:intergenic
CGAACAGCACGAGCACCAAA+AGG	0.514487	1.2:-26827456	None:intergenic
TGGAGGTGATGTTGTTGTTG+TGG	0.519070	1.2:-26827112	None:intergenic
CACGCTTGGCGAAGAGGACA+AGG	0.527749	1.2:-26827360	None:intergenic
GTTGTTGATGTTGTTGATGG+AGG	0.529776	1.2:-26827129	None:intergenic
CTTTCACTCTCACGTTGTAG+TGG	0.531680	1.2:+26827231	MS.gene063207:CDS
CATCAAGACTACCCCACGCT+TGG	0.543485	1.2:-26827374	None:intergenic
GTAGTTGCATTATTTGATGG+TGG	0.544780	1.2:-26827576	None:intergenic
AGAGGACAAGGACATGGTGC+AGG	0.565916	1.2:-26827348	None:intergenic
CTACCCCACGCTTGGCGAAG+AGG	0.567363	1.2:-26827366	None:intergenic
CTACAACGTGAGAGTGAAAG+TGG	0.580478	1.2:-26827228	None:intergenic
GGACAAGGACATGGTGCAGG+TGG	0.588264	1.2:-26827345	None:intergenic
TCATCGTCATCGATGATGAT+GGG	0.604263	1.2:-26827050	None:intergenic
TGTCCTCTTCGCCAAGCGTG+GGG	0.612011	1.2:+26827363	MS.gene063207:CDS
TGGCGAAGAGGACAAGGACA+TGG	0.629659	1.2:-26827354	None:intergenic
CTGATGTTGTAGATGACGAT+GGG	0.633933	1.2:-26827077	None:intergenic
GATTCTCAAGCTAAAGCTAG+AGG	0.638819	1.2:+26827501	MS.gene063207:CDS
GAGAATCCCTAACTTCGCGA+AGG	0.649287	1.2:-26827485	None:intergenic
GCTGATGTTGTAGATGACGA+TGG	0.687351	1.2:-26827078	None:intergenic
TGTTGTAGATGACGATGGGA+TGG	0.693856	1.2:-26827073	None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG	0.746060	1.2:-26827225	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTGTAGTTGCATTATTTGA+TGG	-	chr1.2:26827582-26827601	None:intergenic	30.0%
!	ACTCAGATTTGTCCATTTTA+TGG	+	chr1.2:26827312-26827331	MS.gene063207:CDS	30.0%
!!	GGTTTGTTATGTTGATCATA+GGG	-	chr1.2:26827032-26827051	None:intergenic	30.0%
!!	TTTTGATTCTCGTATCTACT+TGG	-	chr1.2:26827159-26827178	None:intergenic	30.0%
!!!	GAACCTTTGTTTTTCCAAAT+TGG	-	chr1.2:26827290-26827309	None:intergenic	30.0%
	AGAATCAAAAGAGACGAGAT+TGG	+	chr1.2:26827169-26827188	MS.gene063207:CDS	35.0%
	CGACCAATTTGGAAAAACAA+AGG	+	chr1.2:26827284-26827303	MS.gene063207:CDS	35.0%
	GGATTTGGATGTCCATAAAA+TGG	-	chr1.2:26827327-26827346	None:intergenic	35.0%
	GTAGTTGCATTATTTGATGG+TGG	-	chr1.2:26827579-26827598	None:intergenic	35.0%
!	GTTGTTGTTGATGTTGTTGA+TGG	-	chr1.2:26827135-26827154	None:intergenic	35.0%
!!	CTGCTTTTGAAGAAAATGGT+GGG	+	chr1.2:26827418-26827437	MS.gene063207:CDS	35.0%
!!	GGGTTTGTTATGTTGATCAT+AGG	-	chr1.2:26827033-26827052	None:intergenic	35.0%
!!	TTGTTTTTCCAAATTGGTCG+AGG	-	chr1.2:26827284-26827303	None:intergenic	35.0%
!!!	AGAGCTGCTTTTGAAGAAAA+TGG	+	chr1.2:26827414-26827433	MS.gene063207:CDS	35.0%
	ACTTTACCTTCGCGAAGTTA+GGG	+	chr1.2:26827479-26827498	MS.gene063207:CDS	40.0%
	AGACGAGATTGGAACACATT+TGG	+	chr1.2:26827180-26827199	MS.gene063207:CDS	40.0%
	CTGATGTTGTAGATGACGAT+GGG	-	chr1.2:26827080-26827099	None:intergenic	40.0%
	GATTCTCAAGCTAAAGCTAG+AGG	+	chr1.2:26827501-26827520	MS.gene063207:CDS	40.0%
	TCATCGTCATCGATGATGAT+GGG	-	chr1.2:26827053-26827072	None:intergenic	40.0%
!	AAGCCAGAAGCTAATCCTTT+TGG	+	chr1.2:26827441-26827460	MS.gene063207:CDS	40.0%
!	GTAGTCTTGATGCACTCATT+GGG	+	chr1.2:26827385-26827404	MS.gene063207:CDS	40.0%
!	GTTGTTGATGTTGTTGATGG+AGG	-	chr1.2:26827132-26827151	None:intergenic	40.0%
!!	GTTGCATTATTTGATGGTGG+TGG	-	chr1.2:26827576-26827595	None:intergenic	40.0%
!!!	GCTGCTTTTGAAGAAAATGG+TGG	+	chr1.2:26827417-26827436	MS.gene063207:CDS	40.0%
	AGAGTGAAAGTGGTGGTCTA+TGG	-	chr1.2:26827221-26827240	None:intergenic	45.0%
	CGAGGTAACGCAAGAATTCA+AGG	-	chr1.2:26827266-26827285	None:intergenic	45.0%
	CTACAACGTGAGAGTGAAAG+TGG	-	chr1.2:26827231-26827250	None:intergenic	45.0%
	CTTTCACTCTCACGTTGTAG+TGG	+	chr1.2:26827231-26827250	MS.gene063207:CDS	45.0%
	GACTTTACCTTCGCGAAGTT+AGG	+	chr1.2:26827478-26827497	MS.gene063207:CDS	45.0%
	GCTGATGTTGTAGATGACGA+TGG	-	chr1.2:26827081-26827100	None:intergenic	45.0%
	GTCATCGTCATCGATGATGA+TGG	-	chr1.2:26827054-26827073	None:intergenic	45.0%
	GTGGTGGTCTATGGTTTCTT+AGG	-	chr1.2:26827212-26827231	None:intergenic	45.0%
	TGTTGTAGATGACGATGGGA+TGG	-	chr1.2:26827076-26827095	None:intergenic	45.0%
	TTGCGTTACCTCGACCAATT+TGG	+	chr1.2:26827273-26827292	MS.gene063207:CDS	45.0%
!	GGTAGTCTTGATGCACTCAT+TGG	+	chr1.2:26827384-26827403	MS.gene063207:CDS	45.0%
!	TGATGTTGTTGTTGTGGACG+AGG	-	chr1.2:26827109-26827128	None:intergenic	45.0%
!	TGGAGGTGATGTTGTTGTTG+TGG	-	chr1.2:26827115-26827134	None:intergenic	45.0%
!!	GCACCAAAAGGATTAGCTTC+TGG	-	chr1.2:26827447-26827466	None:intergenic	45.0%
!!	GCATTATTTGATGGTGGTGG+TGG	-	chr1.2:26827573-26827592	None:intergenic	45.0%
!!	TTATTTGATGGTGGTGGTGG+TGG	-	chr1.2:26827570-26827589	None:intergenic	45.0%
	CAACGTGAGAGTGAAAGTGG+TGG	-	chr1.2:26827228-26827247	None:intergenic	50.0%
	GAGAATCCCTAACTTCGCGA+AGG	-	chr1.2:26827488-26827507	None:intergenic	50.0%
	AGAGGACAAGGACATGGTGC+AGG	-	chr1.2:26827351-26827370	None:intergenic	55.0%
	CATCAAGACTACCCCACGCT+TGG	-	chr1.2:26827377-26827396	None:intergenic	55.0%
	CGAACAGCACGAGCACCAAA+AGG	-	chr1.2:26827459-26827478	None:intergenic	55.0%
	TGGCGAAGAGGACAAGGACA+TGG	-	chr1.2:26827357-26827376	None:intergenic	55.0%
	TTGTCCTCTTCGCCAAGCGT+GGG	+	chr1.2:26827362-26827381	MS.gene063207:CDS	55.0%
!	GGACATGGTGCAGGTGGATT+TGG	-	chr1.2:26827342-26827361	None:intergenic	55.0%
!!	TTTGATGGTGGTGGTGGTGG+CGG	-	chr1.2:26827567-26827586	None:intergenic	55.0%
	CACGCTTGGCGAAGAGGACA+AGG	-	chr1.2:26827363-26827382	None:intergenic	60.0%
	CTTGTCCTCTTCGCCAAGCG+TGG	+	chr1.2:26827361-26827380	MS.gene063207:CDS	60.0%
	GGACAAGGACATGGTGCAGG+TGG	-	chr1.2:26827348-26827367	None:intergenic	60.0%
	TGTCCTCTTCGCCAAGCGTG+GGG	+	chr1.2:26827363-26827382	MS.gene063207:CDS	60.0%
	CTACCCCACGCTTGGCGAAG+AGG	-	chr1.2:26827369-26827388	None:intergenic	65.0%
!!	GGTGGTGGTGGCGGCTGAGG+CGG	-	chr1.2:26827558-26827577	None:intergenic	75.0%
!!	GGTGGTGGTGGTGGCGGCTG+AGG	-	chr1.2:26827561-26827580	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	26827015	26827614	26827015	ID=MS.gene063207
chr1.2	mRNA	26827015	26827614	26827015	ID=MS.gene063207.t1;Parent=MS.gene063207
chr1.2	exon	26827015	26827614	26827015	ID=MS.gene063207.t1.exon1;Parent=MS.gene063207.t1
chr1.2	CDS	26827015	26827614	26827015	ID=cds.MS.gene063207.t1;Parent=MS.gene063207.t1

Gene Sequence

>MS.gene063207

ATGATGAACACAAACCCTATGATCAACATAACAAACCCATCATCATCGATGACGATGACCATCCCATCGTCATCTACAACATCAGCATCATCCTCGTCCACAACAACAACATCACCTCCATCAACAACATCAACAACAACACCAAGTAGATACGAGAATCAAAAGAGACGAGATTGGAACACATTTGGACAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCTCATGTCCTTGAATTCTTGCGTTACCTCGACCAATTTGGAAAAACAAAGGTTCACACTCAGATTTGTCCATTTTATGGACATCCAAATCCACCTGCACCATGTCCTTGTCCTCTTCGCCAAGCGTGGGGTAGTCTTGATGCACTCATTGGGAGACTTAGAGCTGCTTTTGAAGAAAATGGTGGGAAGCCAGAAGCTAATCCTTTTGGTGCTCGTGCTGTTCGACTTTACCTTCGCGAAGTTAGGGATTCTCAAGCTAAAGCTAGAGGTATTAGTTATGAGAAAAAGAAGCGCAAGCGTCCGCCTCAGCCGCCACCACCACCACCATCAAATAATGCAACTACAACTGATCTTCACTAG

Protein sequence

>MS.gene063207.t1

MMNTNPMINITNPSSSMTMTIPSSSTTSASSSSTTTTSPPSTTSTTTPSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTQICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEANPFGARAVRLYLREVRDSQAKARGISYEKKKRKRPPQPPPPPPSNNATTTDLH