AlfalfaGEDB | MS.gene063365

M. sativa cultivar XinJiangDaYe / MS.gene063365

KEGG: -
GO: -
PFAM: PF12734.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063365.t1	AES66448.1	83.3	78	10	1	1	75	1	78	1.40E-22	115.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063365.t1	G7IH44	83.3	78	10	1	1	75	1	78	9.7e-23	115.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050926	MS.gene063365	0.810719	1.00E-50	-1.69E-46
MS.gene054435	MS.gene063365	0.808481	3.03E-50	-1.69E-46
MS.gene056408	MS.gene063365	0.808804	2.59E-50	-1.69E-46
MS.gene060808	MS.gene063365	0.801857	7.39E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063365.t1	MTR_2g069420	82.051	78	11	1	1	75	1	78	2.58e-34	112
MS.gene063365.t1	MTR_2g069430	81.579	76	13	1	1	75	1	76	2.53e-33	109
MS.gene063365.t1	MTR_1g040735	75.949	79	15	2	1	75	58	136	1.83e-31	106
MS.gene063365.t1	MTR_5g042360	74.684	79	16	2	1	75	1	79	1.70e-30	102
MS.gene063365.t1	MTR_1g040775	74.684	79	16	2	1	75	1	79	1.70e-30	102
MS.gene063365.t1	MTR_4g091090	78.750	80	12	3	1	75	1	80	6.92e-28	95.9
MS.gene063365.t1	MTR_2g069400	59.375	64	21	2	1	63	1	60	4.21e-13	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063365.t1	AT2G32190	53.333	75	31	2	1	75	1	71	4.66e-20	75.9
MS.gene063365.t1	AT2G32210	53.333	75	31	2	1	75	1	71	7.98e-20	75.5
MS.gene063365.t1	AT2G32200	50.000	78	32	3	1	75	35	108	3.16e-16	67.4
MS.gene063365.t1	AT2G32200	50.000	78	32	3	1	75	1	74	5.16e-16	65.9
MS.gene063365.t1	AT3G22240	52.055	73	33	2	3	75	2	72	3.76e-11	53.5
MS.gene063365.t1	AT1G56060	51.667	60	25	3	18	75	14	71	4.82e-11	53.1

Find 31 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCCAAGGGTGATGGTTTC+TGG	0.136023	2.4:-26103303	MS.gene063365:CDS
GTGCCCTTCAGTGCCATCTT+TGG	0.313951	2.4:+26103354	None:intergenic
GAACTTAAAAGCAGATATCT+AGG	0.339148	2.4:+26103245	None:intergenic
ACTGGTGGTGGATATGATAC+TGG	0.355057	2.4:+26103424	None:intergenic
CATCACCCTTGGATGTAGTT+TGG	0.380276	2.4:+26103311	None:intergenic
CCCTTGGATGTAGTTTGGTC+TGG	0.389865	2.4:+26103316	None:intergenic
TGTAACTGCACCACCACCTA+TGG	0.404681	2.4:-26103386	MS.gene063365:CDS
ACATATGGAGCTGTAGATAC+TGG	0.438751	2.4:+26103406	None:intergenic
TAACTTCAACAATAAGCAAC+AGG	0.473699	2.4:-26103449	MS.gene063365:CDS
CTGGTGGTGGATATGATACT+GGG	0.477844	2.4:+26103425	None:intergenic
GGTGGTGGTGCAGTTACATA+TGG	0.482974	2.4:+26103391	None:intergenic
CCTTCCAAAGATGGCACTGA+AGG	0.487973	2.4:-26103358	MS.gene063365:CDS
CTTCCAGAAACCATCACCCT+TGG	0.519384	2.4:+26103300	None:intergenic
ATGGGTTATCCTTCCAAAGA+TGG	0.529377	2.4:-26103367	MS.gene063365:CDS
GGTCTGGAATCGTTTGTTGA+GGG	0.533042	2.4:+26103332	None:intergenic
CCTTCAGTGCCATCTTTGGA+AGG	0.555857	2.4:+26103358	None:intergenic
TCCAGACCAAACTACATCCA+AGG	0.577183	2.4:-26103317	MS.gene063365:CDS
CAAGGGTGATGGTTTCTGGA+AGG	0.584891	2.4:-26103299	MS.gene063365:CDS
AAGGGTGATGGTTTCTGGAA+GGG	0.585272	2.4:-26103298	MS.gene063365:CDS
GAAGGATAACCCATAGGTGG+TGG	0.596519	2.4:+26103376	None:intergenic
GTAACTGCACCACCACCTAT+GGG	0.597034	2.4:-26103385	MS.gene063365:CDS
CTTCCAAAGATGGCACTGAA+GGG	0.598808	2.4:-26103357	MS.gene063365:CDS
TGGTCTGGAATCGTTTGTTG+AGG	0.604125	2.4:+26103331	None:intergenic
AACTTAAAAGCAGATATCTA+GGG	0.606743	2.4:+26103246	None:intergenic
GGAGCTGTAGATACTGGTGG+TGG	0.617769	2.4:+26103412	None:intergenic
CAAACTACATCCAAGGGTGA+TGG	0.635551	2.4:-26103310	MS.gene063365:CDS
CCAGACCAAACTACATCCAA+GGG	0.646562	2.4:-26103316	MS.gene063365:CDS
TCTTTGGAAGGATAACCCAT+AGG	0.652777	2.4:+26103370	None:intergenic
TTGGAAGGATAACCCATAGG+TGG	0.675232	2.4:+26103373	None:intergenic
TGGTGGTGGATATGATACTG+GGG	0.717786	2.4:+26103426	None:intergenic
TATGGAGCTGTAGATACTGG+TGG	0.763094	2.4:+26103409	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAACTTCAACAATAAGCAAC+AGG	-	chr2.4:26103254-26103273	MS.gene063365:CDS	30.0%
	ACATATGGAGCTGTAGATAC+TGG	+	chr2.4:26103300-26103319	None:intergenic	40.0%
!	ATGGGTTATCCTTCCAAAGA+TGG	-	chr2.4:26103336-26103355	MS.gene063365:CDS	40.0%
!	TCTTTGGAAGGATAACCCAT+AGG	+	chr2.4:26103336-26103355	None:intergenic	40.0%
	CAAACTACATCCAAGGGTGA+TGG	-	chr2.4:26103393-26103412	MS.gene063365:CDS	45.0%
	CATCACCCTTGGATGTAGTT+TGG	+	chr2.4:26103395-26103414	None:intergenic	45.0%
	CCAGACCAAACTACATCCAA+GGG	-	chr2.4:26103387-26103406	MS.gene063365:CDS	45.0%
	TATGGAGCTGTAGATACTGG+TGG	+	chr2.4:26103297-26103316	None:intergenic	45.0%
	TCCAGACCAAACTACATCCA+AGG	-	chr2.4:26103386-26103405	MS.gene063365:CDS	45.0%
	TTGGAAGGATAACCCATAGG+TGG	+	chr2.4:26103333-26103352	None:intergenic	45.0%
!	AAGGGTGATGGTTTCTGGAA+GGG	-	chr2.4:26103405-26103424	MS.gene063365:CDS	45.0%
!	CTTCCAAAGATGGCACTGAA+GGG	-	chr2.4:26103346-26103365	MS.gene063365:CDS	45.0%
!	GGTCTGGAATCGTTTGTTGA+GGG	+	chr2.4:26103374-26103393	None:intergenic	45.0%
!	TGGTCTGGAATCGTTTGTTG+AGG	+	chr2.4:26103375-26103394	None:intergenic	45.0%
!!	ACTGGTGGTGGATATGATAC+TGG	+	chr2.4:26103282-26103301	None:intergenic	45.0%
!!	CTGGTGGTGGATATGATACT+GGG	+	chr2.4:26103281-26103300	None:intergenic	45.0%
!!	TGGTGGTGGATATGATACTG+GGG	+	chr2.4:26103280-26103299	None:intergenic	45.0%
	CCCTTGGATGTAGTTTGGTC+TGG	+	chr2.4:26103390-26103409	None:intergenic	50.0%
	CCTTCAGTGCCATCTTTGGA+AGG	+	chr2.4:26103348-26103367	None:intergenic	50.0%
	CTTCCAGAAACCATCACCCT+TGG	+	chr2.4:26103406-26103425	None:intergenic	50.0%
	GAAGGATAACCCATAGGTGG+TGG	+	chr2.4:26103330-26103349	None:intergenic	50.0%
	GTAACTGCACCACCACCTAT+GGG	-	chr2.4:26103318-26103337	MS.gene063365:CDS	50.0%
	TGTAACTGCACCACCACCTA+TGG	-	chr2.4:26103317-26103336	MS.gene063365:CDS	50.0%
!	CAAGGGTGATGGTTTCTGGA+AGG	-	chr2.4:26103404-26103423	MS.gene063365:CDS	50.0%
!	CATCCAAGGGTGATGGTTTC+TGG	-	chr2.4:26103400-26103419	MS.gene063365:CDS	50.0%
!	CCTTCCAAAGATGGCACTGA+AGG	-	chr2.4:26103345-26103364	MS.gene063365:CDS	50.0%
!	GGTGGTGGTGCAGTTACATA+TGG	+	chr2.4:26103315-26103334	None:intergenic	50.0%
	GGAGCTGTAGATACTGGTGG+TGG	+	chr2.4:26103294-26103313	None:intergenic	55.0%
	GTGCCCTTCAGTGCCATCTT+TGG	+	chr2.4:26103352-26103371	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	26103249	26103476	26103249	ID=MS.gene063365
chr2.4	mRNA	26103249	26103476	26103249	ID=MS.gene063365.t1;Parent=MS.gene063365
chr2.4	exon	26103249	26103476	26103249	ID=MS.gene063365.t1.exon1;Parent=MS.gene063365.t1
chr2.4	CDS	26103249	26103476	26103249	ID=cds.MS.gene063365.t1;Parent=MS.gene063365.t1

Gene Sequence	>MS.gene063365 ATGAGTAACTTCAACAATAAGCAACAGGCCCCAGTATCATATCCACCACCAGTATCTACAGCTCCATATGTAACTGCACCACCACCTATGGGTTATCCTTCCAAAGATGGCACTGAAGGGCACCCTCAACAAACGATTCCAGACCAAACTACATCCAAGGGTGATGGTTTCTGGAAGGGATGCTGTGCTGCTATGTGTTGCTGTTGTGCCCTAGATATCTGCTTTTAA
Protein sequence	>MS.gene063365.t1 MSNFNNKQQAPVSYPPPVSTAPYVTAPPPMGYPSKDGTEGHPQQTIPDQTTSKGDGFWKGCCAAMCCCCALDICF