Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063532.t1 | XP_003609182.2 | 84.8 | 145 | 19 | 2 | 1 | 142 | 1 | 145 | 1.90E-54 | 221.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063532.t1 | G7JT57 | 84.8 | 145 | 19 | 2 | 1 | 142 | 1 | 145 | 1.4e-54 | 221.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene063532 | 0.808534 | 2.95E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene063532 | 0.820068 | 8.34E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene063532 | 0.807777 | 4.28E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene063532 | 0.803559 | 3.29E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene063532 | 0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene063532 | 0.817564 | 3.09E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene063532 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene063532 | 0.812503 | 4.10E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063532.t1 | MTR_4g112870 | 92.414 | 145 | 8 | 2 | 1 | 142 | 1 | 145 | 2.63e-60 | 182 |
| MS.gene063532.t1 | MTR_7g017790 | 41.593 | 113 | 54 | 4 | 7 | 109 | 8 | 118 | 1.18e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.144324 | 8.1:+15380904 | MS.gene063532:CDS |
| TCATCACTTTCATCATCTTC+TGG | 0.273861 | 8.1:-15380877 | None:intergenic |
| CAACAACATGGGAAAGACTA+TGG | 0.338944 | 8.1:+15380934 | MS.gene063532:CDS |
| TCTGGATGGACCATGTATAT+TGG | 0.342364 | 8.1:+15380781 | MS.gene063532:CDS |
| TCTTTCCCATGTTGTTGAAT+TGG | 0.342834 | 8.1:-15380928 | None:intergenic |
| TAAAGGCAAGCCTTCAGTTC+AGG | 0.344053 | 8.1:+15381107 | MS.gene063532:CDS |
| TGGTTTCTGATGCTTCTTCT+GGG | 0.357913 | 8.1:+15380905 | MS.gene063532:CDS |
| TCAGTTCAGGGTGGTGGCAA+GGG | 0.368073 | 8.1:+15381120 | MS.gene063532:CDS |
| CTGGATGGACCATGTATATT+GGG | 0.393689 | 8.1:+15380782 | MS.gene063532:CDS |
| GAATGTGAAAGCAGTGAATC+TGG | 0.408814 | 8.1:+15380763 | MS.gene063532:CDS |
| TGATGAAAGTGATGATTCTA+TGG | 0.423100 | 8.1:+15380885 | MS.gene063532:CDS |
| GTGGTGGCAAGGGGAGAAAA+AGG | 0.440492 | 8.1:+15381130 | MS.gene063532:CDS |
| TTCAGTTCAGGGTGGTGGCA+AGG | 0.451207 | 8.1:+15381119 | MS.gene063532:CDS |
| AAGAAGTTTGCAGATGGTAA+TGG | 0.467923 | 8.1:+15381084 | MS.gene063532:CDS |
| GCAAAGGAACGGAAAGAAAG+TGG | 0.505274 | 8.1:+15381050 | MS.gene063532:CDS |
| TTGCCACCACCCTGAACTGA+AGG | 0.505883 | 8.1:-15381117 | None:intergenic |
| TTTGCAGATGGTAATGGTAA+AGG | 0.508477 | 8.1:+15381090 | MS.gene063532:CDS |
| AAAGGCAAGCCTTCAGTTCA+GGG | 0.509054 | 8.1:+15381108 | MS.gene063532:CDS |
| CATCACTATCATCTTCTATG+TGG | 0.530187 | 8.1:-15380807 | None:intergenic |
| ATTGATAACATGGATGATGA+AGG | 0.534994 | 8.1:+15380835 | MS.gene063532:CDS |
| AAGCCTTCAGTTCAGGGTGG+TGG | 0.535858 | 8.1:+15381114 | MS.gene063532:CDS |
| CAGTTCAGGGTGGTGGCAAG+GGG | 0.546581 | 8.1:+15381121 | MS.gene063532:CDS |
| ATCACTATCATCTTCTATGT+GGG | 0.557807 | 8.1:-15380806 | None:intergenic |
| CACATAGAAGATGATAGTGA+TGG | 0.575886 | 8.1:+15380808 | MS.gene063532:CDS |
| TCTGGGCCAATTCAACAACA+TGG | 0.586571 | 8.1:+15380922 | MS.gene063532:CDS |
| TGATGGAGATATTGATAACA+TGG | 0.587302 | 8.1:+15380825 | MS.gene063532:CDS |
| ATAAAACTTTGGAAAAGCAA+AGG | 0.605010 | 8.1:+15381034 | MS.gene063532:CDS |
| AGACTATGGTTTCAAGCAAG+TGG | 0.606860 | 8.1:+15380948 | MS.gene063532:CDS |
| AAAGAGAAGAAGTTTGCAGA+TGG | 0.612808 | 8.1:+15381078 | MS.gene063532:CDS |
| TATGTGGGACCCAATATACA+TGG | 0.618706 | 8.1:-15380791 | None:intergenic |
| GTGAAAGCAGTGAATCTGGA+TGG | 0.629063 | 8.1:+15380767 | MS.gene063532:CDS |
| CTGGGCCAATTCAACAACAT+GGG | 0.629938 | 8.1:+15380923 | MS.gene063532:CDS |
| CTATGGTTTCAAGCAAGTGG+TGG | 0.638759 | 8.1:+15380951 | MS.gene063532:CDS |
| ACTTTGGAAAAGCAAAGGAA+CGG | 0.650922 | 8.1:+15381039 | MS.gene063532:CDS |
| GGCAAGCCTTCAGTTCAGGG+TGG | 0.742474 | 8.1:+15381111 | MS.gene063532:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAAAGCAAATAAAACTT+TGG | + | chr8.1:15381023-15381042 | MS.gene063532:CDS | 20.0% |
| ! | ATGAAACCATCCAAAAAATT+TGG | + | chr8.1:15380730-15380749 | MS.gene063532:CDS | 25.0% |
| !! | ATAAAACTTTGGAAAAGCAA+AGG | + | chr8.1:15381034-15381053 | MS.gene063532:CDS | 25.0% |
| ATCACTATCATCTTCTATGT+GGG | - | chr8.1:15380809-15380828 | None:intergenic | 30.0% | |
| CAAAAAATTTGGAAGTGAAG+AGG | + | chr8.1:15380741-15380760 | MS.gene063532:CDS | 30.0% | |
| TGATGAAAGTGATGATTCTA+TGG | + | chr8.1:15380885-15380904 | MS.gene063532:CDS | 30.0% | |
| ! | ATTGATAACATGGATGATGA+AGG | + | chr8.1:15380835-15380854 | MS.gene063532:CDS | 30.0% |
| ! | TGATGGAGATATTGATAACA+TGG | + | chr8.1:15380825-15380844 | MS.gene063532:CDS | 30.0% |
| !!! | CTCTTCACTTCCAAATTTTT+TGG | - | chr8.1:15380743-15380762 | None:intergenic | 30.0% |
| !!! | TCACTTCCAAATTTTTTGGA+TGG | - | chr8.1:15380739-15380758 | None:intergenic | 30.0% |
| AAAGAGAAGAAGTTTGCAGA+TGG | + | chr8.1:15381078-15381097 | MS.gene063532:CDS | 35.0% | |
| AAGAAGTTTGCAGATGGTAA+TGG | + | chr8.1:15381084-15381103 | MS.gene063532:CDS | 35.0% | |
| CATCACTATCATCTTCTATG+TGG | - | chr8.1:15380810-15380829 | None:intergenic | 35.0% | |
| TCATCACTTTCATCATCTTC+TGG | - | chr8.1:15380880-15380899 | None:intergenic | 35.0% | |
| TCTTTCCCATGTTGTTGAAT+TGG | - | chr8.1:15380931-15380950 | None:intergenic | 35.0% | |
| ! | ACTTTGGAAAAGCAAAGGAA+CGG | + | chr8.1:15381039-15381058 | MS.gene063532:CDS | 35.0% |
| ! | CACATAGAAGATGATAGTGA+TGG | + | chr8.1:15380808-15380827 | MS.gene063532:CDS | 35.0% |
| !! | TTTGCAGATGGTAATGGTAA+AGG | + | chr8.1:15381090-15381109 | MS.gene063532:CDS | 35.0% |
| AGACTATGGTTTCAAGCAAG+TGG | + | chr8.1:15380948-15380967 | MS.gene063532:CDS | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | + | chr8.1:15380904-15380923 | MS.gene063532:CDS | 40.0% | |
| CAACAACATGGGAAAGACTA+TGG | + | chr8.1:15380934-15380953 | MS.gene063532:CDS | 40.0% | |
| CTGGATGGACCATGTATATT+GGG | + | chr8.1:15380782-15380801 | MS.gene063532:CDS | 40.0% | |
| GAATGTGAAAGCAGTGAATC+TGG | + | chr8.1:15380763-15380782 | MS.gene063532:CDS | 40.0% | |
| TATGTGGGACCCAATATACA+TGG | - | chr8.1:15380794-15380813 | None:intergenic | 40.0% | |
| TCTGGATGGACCATGTATAT+TGG | + | chr8.1:15380781-15380800 | MS.gene063532:CDS | 40.0% | |
| TGGTTTCTGATGCTTCTTCT+GGG | + | chr8.1:15380905-15380924 | MS.gene063532:CDS | 40.0% | |
| AAAGGCAAGCCTTCAGTTCA+GGG | + | chr8.1:15381108-15381127 | MS.gene063532:CDS | 45.0% | |
| CTATGGTTTCAAGCAAGTGG+TGG | + | chr8.1:15380951-15380970 | MS.gene063532:CDS | 45.0% | |
| CTGGGCCAATTCAACAACAT+GGG | + | chr8.1:15380923-15380942 | MS.gene063532:CDS | 45.0% | |
| GCAAAGGAACGGAAAGAAAG+TGG | + | chr8.1:15381050-15381069 | MS.gene063532:CDS | 45.0% | |
| GTGAAAGCAGTGAATCTGGA+TGG | + | chr8.1:15380767-15380786 | MS.gene063532:CDS | 45.0% | |
| TAAAGGCAAGCCTTCAGTTC+AGG | + | chr8.1:15381107-15381126 | MS.gene063532:CDS | 45.0% | |
| TCTGGGCCAATTCAACAACA+TGG | + | chr8.1:15380922-15380941 | MS.gene063532:CDS | 45.0% | |
| AAGCCTTCAGTTCAGGGTGG+TGG | + | chr8.1:15381114-15381133 | MS.gene063532:CDS | 55.0% | |
| GTGGTGGCAAGGGGAGAAAA+AGG | + | chr8.1:15381130-15381149 | MS.gene063532:CDS | 55.0% | |
| TTGCCACCACCCTGAACTGA+AGG | - | chr8.1:15381120-15381139 | None:intergenic | 55.0% | |
| ! | TCAGTTCAGGGTGGTGGCAA+GGG | + | chr8.1:15381120-15381139 | MS.gene063532:CDS | 55.0% |
| ! | TTCAGTTCAGGGTGGTGGCA+AGG | + | chr8.1:15381119-15381138 | MS.gene063532:CDS | 55.0% |
| GGCAAGCCTTCAGTTCAGGG+TGG | + | chr8.1:15381111-15381130 | MS.gene063532:CDS | 60.0% | |
| ! | CAGTTCAGGGTGGTGGCAAG+GGG | + | chr8.1:15381121-15381140 | MS.gene063532:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 15380730 | 15381158 | 15380730 | ID=MS.gene063532 |
| chr8.1 | mRNA | 15380730 | 15381158 | 15380730 | ID=MS.gene063532.t1;Parent=MS.gene063532 |
| chr8.1 | exon | 15380730 | 15381158 | 15380730 | ID=MS.gene063532.t1.exon1;Parent=MS.gene063532.t1 |
| chr8.1 | CDS | 15380730 | 15381158 | 15380730 | ID=cds.MS.gene063532.t1;Parent=MS.gene063532.t1 |
| Gene Sequence |
| Protein sequence |
