AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056215


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056215.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene056215.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene056215.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056215.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene056215.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGGACTTTGTATGGTTT+CGG 0.343848 8.1:+65412952 MS.gene056215:CDS
TCGCTGGCTTTGTGATTCCT+TGG 0.395127 8.1:-65412750 None:intergenic
GGGAGGTAAGGGACTAGGAA+AGG 0.428277 8.1:+65412687 MS.gene056215:CDS
AGACAAGGAAGGACTTTGTA+TGG 0.433181 8.1:+65412946 MS.gene056215:CDS
CTCGCTAATCGCCGAATCGC+TGG 0.442530 8.1:-65412766 None:intergenic
GGAGGTAAGGGACTAGGAAA+GGG 0.452874 8.1:+65412688 MS.gene056215:CDS
GAGGAAGACGGTTACTGCTA+TGG 0.485067 8.1:+65412903 MS.gene056215:CDS
AGGTCGTGGAAAGGGAGGTA+AGG 0.492819 8.1:+65412675 MS.gene056215:CDS
GATTCTCCATTGAAAATGTC+AGG 0.496148 8.1:+65412655 None:intergenic
GAATCACAAAGCCAGCGATT+CGG 0.499665 8.1:+65412755 MS.gene056215:CDS
GGAAAGGGAGGTAAGGGACT+AGG 0.521051 8.1:+65412682 MS.gene056215:CDS
AAAATGTCAGGTCGTGGAAA+GGG 0.538992 8.1:+65412667 None:intergenic
CCATTGAAAATGTCAGGTCG+TGG 0.548627 8.1:+65412661 None:intergenic
GGTAAGGGACTAGGAAAGGG+AGG 0.563878 8.1:+65412691 MS.gene056215:CDS
GGTCGTGGAAAGGGAGGTAA+GGG 0.564245 8.1:+65412676 MS.gene056215:CDS
GAAAATGTCAGGTCGTGGAA+AGG 0.584382 8.1:+65412666 None:intergenic
CGGCGATTAGCGAGAAGAGG+AGG 0.588328 8.1:+65412775 MS.gene056215:CDS
AGGACTTTGTATGGTTTCGG+AGG 0.599906 8.1:+65412955 MS.gene056215:CDS
ATGCTTTGAAGAGACAAGGA+AGG 0.603293 8.1:+65412935 MS.gene056215:CDS
GTGCTTCGCGACAACATCCA+AGG 0.609211 8.1:+65412733 MS.gene056215:CDS
GGAGGTGTGAAGAGAATCAG+CGG 0.613587 8.1:+65412793 MS.gene056215:CDS
ATTCGGCGATTAGCGAGAAG+AGG 0.620365 8.1:+65412772 MS.gene056215:CDS
GTTTATGCTTTGAAGAGACA+AGG 0.627217 8.1:+65412931 MS.gene056215:CDS
TGAACATGCGAGGAGGAAGA+CGG 0.628798 8.1:+65412891 MS.gene056215:CDS
ATGTCAGGTCGTGGAAAGGG+AGG 0.643397 8.1:+65412670 MS.gene056215:CDS
GAGGTGTGAAGAGAATCAGC+GGG 0.664332 8.1:+65412794 MS.gene056215:CDS
TAGGAAAGGGAGGTGCAAAG+AGG 0.683932 8.1:+65412701 MS.gene056215:CDS
TTGATTTATGAAGAAACTCG+CGG 0.688782 8.1:+65412817 MS.gene056215:CDS
CGTATACTGAACATGCGAGG+AGG 0.708048 8.1:+65412884 MS.gene056215:CDS
TTACGTATACTGAACATGCG+AGG 0.714123 8.1:+65412881 MS.gene056215:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATTTATGAAGAAACTCG+CGG + chr8.1:65412817-65412836 MS.gene056215:CDS 30.0%
! CGGTGTATTGAAGATTTTCT+TGG + chr8.1:65412837-65412856 MS.gene056215:CDS 35.0%
!! GTTTATGCTTTGAAGAGACA+AGG + chr8.1:65412931-65412950 MS.gene056215:CDS 35.0%
AGACAAGGAAGGACTTTGTA+TGG + chr8.1:65412946-65412965 MS.gene056215:CDS 40.0%
GGAAGGACTTTGTATGGTTT+CGG + chr8.1:65412952-65412971 MS.gene056215:CDS 40.0%
TTACGTATACTGAACATGCG+AGG + chr8.1:65412881-65412900 MS.gene056215:CDS 40.0%
!! ATGCTTTGAAGAGACAAGGA+AGG + chr8.1:65412935-65412954 MS.gene056215:CDS 40.0%
AGGACTTTGTATGGTTTCGG+AGG + chr8.1:65412955-65412974 MS.gene056215:CDS 45.0%
GAATCACAAAGCCAGCGATT+CGG + chr8.1:65412755-65412774 MS.gene056215:CDS 45.0%
ATTCGGCGATTAGCGAGAAG+AGG + chr8.1:65412772-65412791 MS.gene056215:CDS 50.0%
CGTATACTGAACATGCGAGG+AGG + chr8.1:65412884-65412903 MS.gene056215:CDS 50.0%
GAGGAAGACGGTTACTGCTA+TGG + chr8.1:65412903-65412922 MS.gene056215:CDS 50.0%
GAGGTGTGAAGAGAATCAGC+GGG + chr8.1:65412794-65412813 MS.gene056215:CDS 50.0%
GGAGGTAAGGGACTAGGAAA+GGG + chr8.1:65412688-65412707 MS.gene056215:CDS 50.0%
TGAACATGCGAGGAGGAAGA+CGG + chr8.1:65412891-65412910 MS.gene056215:CDS 50.0%
! GGAGGTGTGAAGAGAATCAG+CGG + chr8.1:65412793-65412812 MS.gene056215:CDS 50.0%
! TAGGAAAGGGAGGTGCAAAG+AGG + chr8.1:65412701-65412720 MS.gene056215:CDS 50.0%
!! TCGCTGGCTTTGTGATTCCT+TGG - chr8.1:65412753-65412772 None:intergenic 50.0%
AGGTCGTGGAAAGGGAGGTA+AGG + chr8.1:65412675-65412694 MS.gene056215:CDS 55.0%
ATGTCAGGTCGTGGAAAGGG+AGG + chr8.1:65412670-65412689 MS.gene056215:CDS 55.0%
GGAAAGGGAGGTAAGGGACT+AGG + chr8.1:65412682-65412701 MS.gene056215:CDS 55.0%
GGGAGGTAAGGGACTAGGAA+AGG + chr8.1:65412687-65412706 MS.gene056215:CDS 55.0%
GGTAAGGGACTAGGAAAGGG+AGG + chr8.1:65412691-65412710 MS.gene056215:CDS 55.0%
GGTCGTGGAAAGGGAGGTAA+GGG + chr8.1:65412676-65412695 MS.gene056215:CDS 55.0%
GTGCTTCGCGACAACATCCA+AGG + chr8.1:65412733-65412752 MS.gene056215:CDS 55.0%
CGGCGATTAGCGAGAAGAGG+AGG + chr8.1:65412775-65412794 MS.gene056215:CDS 60.0%
CTCGCTAATCGCCGAATCGC+TGG - chr8.1:65412769-65412788 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 65412670 65412981 65412670 ID=MS.gene056215
chr8.1 mRNA 65412670 65412981 65412670 ID=MS.gene056215.t1;Parent=MS.gene056215
chr8.1 exon 65412670 65412981 65412670 ID=MS.gene056215.t1.exon1;Parent=MS.gene056215.t1
chr8.1 CDS 65412670 65412981 65412670 ID=cds.MS.gene056215.t1;Parent=MS.gene056215.t1
Gene Sequence

>MS.gene056215

ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGGCATCGCAAAGTGCTTCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGGCGATTAGCGAGAAGAGGAGGTGTGAAGAGAATCAGCGGGTTGATTTATGAAGAAACTCGCGGTGTATTGAAGATTTTCTTGGAGAATGTGATAAGAGATGCTGTTACGTATACTGAACATGCGAGGAGGAAGACGGTTACTGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTTTGTATGGTTTCGGAGGTTGA

Protein sequence

>MS.gene056215.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG