Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063555.t1 | XP_003612270.1 | 89.9 | 179 | 12 | 2 | 1 | 173 | 7 | 185 | 3.00E-81 | 311.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063555.t1 | Q38853 | 53.1 | 143 | 57 | 4 | 41 | 173 | 40 | 182 | 1.7e-32 | 140.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063555.t1 | B7FGV4 | 89.9 | 179 | 12 | 2 | 1 | 173 | 7 | 185 | 2.1e-81 | 311.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene063555 | 0.809259 | 2.07E-50 | -1.69E-46 |
| MS.gene058082 | MS.gene063555 | 0.80106 | 1.08E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene063555 | 0.810682 | 1.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATGTCCCTTATTAAATT+TGG | 0.146508 | 5.2:-14515198 | None:intergenic |
| AAAATCTTCCAAATTTAATA+AGG | 0.178231 | 5.2:+14515190 | MS.gene063555:CDS |
| TCTAGCTGGCCATAAGTATT+TGG | 0.181385 | 5.2:+14515438 | MS.gene063555:CDS |
| ACCTTACATCCAAATACTTA+TGG | 0.228445 | 5.2:-14515447 | None:intergenic |
| GTGTGGTTTAAAGGGGAATT+TGG | 0.241136 | 5.2:+14515360 | MS.gene063555:CDS |
| AAGCACGATGTGTGGTTTAA+AGG | 0.244947 | 5.2:+14515351 | MS.gene063555:intron |
| AGCACGATGTGTGGTTTAAA+GGG | 0.328888 | 5.2:+14515352 | MS.gene063555:intron |
| GATCCAACTTTGTACATATA+AGG | 0.335693 | 5.2:-14515801 | None:intergenic |
| TATGCTGCTTGGACTCAAAA+TGG | 0.348576 | 5.2:+14516689 | MS.gene063555:CDS |
| AAATCTTCCAAATTTAATAA+GGG | 0.364373 | 5.2:+14515191 | MS.gene063555:CDS |
| ATTAGAGTGTGACCTTGTTA+TGG | 0.398979 | 5.2:-14515029 | None:intergenic |
| GGGAATTTGGAGGAAGTTGT+TGG | 0.404121 | 5.2:+14515373 | MS.gene063555:CDS |
| GCCATAAGTATTTGGATGTA+AGG | 0.427420 | 5.2:+14515446 | MS.gene063555:CDS |
| TAGCTGGTGGTTATGCTGCT+TGG | 0.436274 | 5.2:+14516678 | MS.gene063555:CDS |
| ACACGCACTGGCACTGAAGT+TGG | 0.437130 | 5.2:-14515400 | None:intergenic |
| ACCAGCTATGTCTGTGAGTC+CGG | 0.440393 | 5.2:-14516663 | None:intergenic |
| AACCAAAGTGTTCCAACTTC+CGG | 0.448542 | 5.2:+14515234 | MS.gene063555:CDS |
| GTGTTCTGCCGTAGAATTAG+AGG | 0.450733 | 5.2:-14515004 | None:intergenic |
| TTTAATGCTGGACACGCTCC+TGG | 0.480736 | 5.2:+14515766 | MS.gene063555:CDS |
| TGCATTGTATAGGGGTTCAC+CGG | 0.511879 | 5.2:+14516644 | MS.gene063555:intron |
| GCTCACGAGCTTCTTCTAGC+TGG | 0.514761 | 5.2:+14515424 | MS.gene063555:CDS |
| AGGGTTGTCAGCTTGGGAAA+AGG | 0.515357 | 5.2:+14516239 | MS.gene063555:intron |
| AGCTCGTGAGCTACACGCAC+TGG | 0.517304 | 5.2:-14515412 | None:intergenic |
| TACCGGAAGTTGGAACACTT+TGG | 0.532325 | 5.2:-14515236 | None:intergenic |
| ATTCACGTCCTCTAATTCTA+CGG | 0.536092 | 5.2:+14514996 | MS.gene063555:CDS |
| ACCACGGAAGAGTTTAATGC+TGG | 0.548422 | 5.2:+14515754 | MS.gene063555:CDS |
| TGGTTTAAAGGGGAATTTGG+AGG | 0.552343 | 5.2:+14515363 | MS.gene063555:CDS |
| GCTTGGGAAAAGGTCTATGA+TGG | 0.555495 | 5.2:+14516249 | MS.gene063555:CDS |
| ACCGGACTCACAGACATAGC+TGG | 0.570324 | 5.2:+14516662 | MS.gene063555:CDS |
| GCAAGCGGCGGAGACATCAA+AGG | 0.618010 | 5.2:-14514969 | None:intergenic |
| GCACGATGTGTGGTTTAAAG+GGG | 0.620601 | 5.2:+14515353 | MS.gene063555:intron |
| ATAAGGTATGTAATCGCACC+AGG | 0.636223 | 5.2:-14515784 | None:intergenic |
| TCCAGCATTAAACTCTTCCG+TGG | 0.640037 | 5.2:-14515755 | None:intergenic |
| GGCAGAACACGACCATAACA+AGG | 0.641310 | 5.2:+14515017 | MS.gene063555:CDS |
| TATTGTGATGTGCAGGACCA+CGG | 0.654081 | 5.2:+14515738 | MS.gene063555:intron |
| AGGACGTGAATGAAAGCAAG+CGG | 0.673997 | 5.2:-14514984 | None:intergenic |
| ACGTGAATGAAAGCAAGCGG+CGG | 0.698419 | 5.2:-14514981 | None:intergenic |
| GGACTCACAGACATAGCTGG+TGG | 0.717723 | 5.2:+14516665 | MS.gene063555:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCTTCCAAATTTAATA+AGG | + | chr5.2:14515190-14515209 | MS.gene063555:CDS | 15.0% |
| !! | AAATCTTCCAAATTTAATAA+GGG | + | chr5.2:14515191-14515210 | MS.gene063555:CDS | 15.0% |
| !! | ATATGAATAGATTAGAAAAA+AGG | - | chr5.2:14516018-14516037 | None:intergenic | 15.0% |
| !! | CAAAACATTAGTATATTAAT+CGG | - | chr5.2:14515670-14515689 | None:intergenic | 15.0% |
| !! | AAAATAACATACAAATCTCA+TGG | - | chr5.2:14516175-14516194 | None:intergenic | 20.0% |
| !! | AGTATATAGATATAGAAGAA+TGG | - | chr5.2:14515059-14515078 | None:intergenic | 20.0% |
| !! | TAAAGTAACCAATTTAATCA+AGG | + | chr5.2:14515469-14515488 | MS.gene063555:intron | 20.0% |
| !! | TAAGAGATATATAATACTCT+TGG | + | chr5.2:14515907-14515926 | MS.gene063555:intron | 20.0% |
| !! | TATTTAAGCCTTGATTAAAT+TGG | - | chr5.2:14515480-14515499 | None:intergenic | 20.0% |
| !! | TGTAATATTCTTAGTGTTTA+GGG | + | chr5.2:14515856-14515875 | MS.gene063555:intron | 20.0% |
| !! | TTGTAATATTCTTAGTGTTT+AGG | + | chr5.2:14515855-14515874 | MS.gene063555:intron | 20.0% |
| !!! | AGTTGTTTTGATTAATCTAA+TGG | - | chr5.2:14515933-14515952 | None:intergenic | 20.0% |
| !!! | CATAATAGTTTTGTAGTTTT+AGG | - | chr5.2:14515543-14515562 | None:intergenic | 20.0% |
| !!! | CGATTAATATACTAATGTTT+TGG | + | chr5.2:14515668-14515687 | MS.gene063555:intron | 20.0% |
| !!! | TTAACTTCTTGAAAATTTTG+AGG | + | chr5.2:14516594-14516613 | MS.gene063555:intron | 20.0% |
| !!! | TTATTTTTGTTTACACTTTC+AGG | - | chr5.2:14516502-14516521 | None:intergenic | 20.0% |
| !!! | TTTTCATCAATAATTAGTCT+TGG | + | chr5.2:14516534-14516553 | MS.gene063555:intron | 20.0% |
| ! | AGATCAAAAAGAGATCAAAT+TGG | + | chr5.2:14515978-14515997 | MS.gene063555:intron | 25.0% |
| ! | ATACCTTATATGTACAAAGT+TGG | + | chr5.2:14515798-14515817 | MS.gene063555:intron | 25.0% |
| ! | ATTAGAAAAAAGGACACTAA+TGG | - | chr5.2:14516008-14516027 | None:intergenic | 25.0% |
| ! | CATTAGCAGATGAATTAATT+TGG | - | chr5.2:14516206-14516225 | None:intergenic | 25.0% |
| ! | GTAAGAATTTGAAAACACAT+AGG | - | chr5.2:14515133-14515152 | None:intergenic | 25.0% |
| ! | TAGATTAATCAAAACAACTG+AGG | + | chr5.2:14515934-14515953 | MS.gene063555:intron | 25.0% |
| ! | TATTGATGAAAAAAGTAGCA+CGG | - | chr5.2:14516526-14516545 | None:intergenic | 25.0% |
| ! | TCTATGTCCCTTATTAAATT+TGG | - | chr5.2:14515201-14515220 | None:intergenic | 25.0% |
| !! | GTTAATAAAGTATCTATCAC+TGG | - | chr5.2:14516579-14516598 | None:intergenic | 25.0% |
| !!! | AATGTTTTGGAATTGTGATT+TGG | + | chr5.2:14515681-14515700 | MS.gene063555:intron | 25.0% |
| !!! | AATTGTTTTGCATTGTATAG+GGG | + | chr5.2:14516636-14516655 | MS.gene063555:intron | 25.0% |
| !!! | ACAATTGTTTTGCATTGTAT+AGG | + | chr5.2:14516634-14516653 | MS.gene063555:intron | 25.0% |
| !!! | CAATTGTTTTGCATTGTATA+GGG | + | chr5.2:14516635-14516654 | MS.gene063555:intron | 25.0% |
| AAACTCAAACAAGAAGTGAA+AGG | - | chr5.2:14516441-14516460 | None:intergenic | 30.0% | |
| AACTCAAACAAGAAGTGAAA+GGG | - | chr5.2:14516440-14516459 | None:intergenic | 30.0% | |
| AAGTATCTATCACTGGAAAT+TGG | - | chr5.2:14516572-14516591 | None:intergenic | 30.0% | |
| AATTATGTGTTCATGCATAC+AGG | + | chr5.2:14516056-14516075 | MS.gene063555:intron | 30.0% | |
| ACCTTACATCCAAATACTTA+TGG | - | chr5.2:14515450-14515469 | None:intergenic | 30.0% | |
| ATTATGTGTTCATGCATACA+GGG | + | chr5.2:14516057-14516076 | MS.gene063555:intron | 30.0% | |
| GATCCAACTTTGTACATATA+AGG | - | chr5.2:14515804-14515823 | None:intergenic | 30.0% | |
| TATATGTACAAAGTTGGATC+AGG | + | chr5.2:14515804-14515823 | MS.gene063555:intron | 30.0% | |
| ! | ATCTTCTTTTCTAAACTGTG+AGG | - | chr5.2:14516117-14516136 | None:intergenic | 30.0% |
| ! | GAACAGCAATTTTGTTAAAG+AGG | + | chr5.2:14516088-14516107 | MS.gene063555:CDS | 30.0% |
| !! | CTAATGTTATTGCTTTTGCA+GGG | + | chr5.2:14516220-14516239 | MS.gene063555:intron | 30.0% |
| !! | TCAACACTTTTTACACTTAC+CGG | - | chr5.2:14515256-14515275 | None:intergenic | 30.0% |
| ACTCAAACAAGAAGTGAAAG+GGG | - | chr5.2:14516439-14516458 | None:intergenic | 35.0% | |
| ATTAGAGTGTGACCTTGTTA+TGG | - | chr5.2:14515032-14515051 | None:intergenic | 35.0% | |
| ATTCACGTCCTCTAATTCTA+CGG | + | chr5.2:14514996-14515015 | MS.gene063555:CDS | 35.0% | |
| CAACTGAGGTTTGATAGAAT+AGG | + | chr5.2:14515948-14515967 | MS.gene063555:intron | 35.0% | |
| CACATCACAATAAACAGGTA+TGG | - | chr5.2:14515730-14515749 | None:intergenic | 35.0% | |
| GCCATAAGTATTTGGATGTA+AGG | + | chr5.2:14515446-14515465 | MS.gene063555:CDS | 35.0% | |
| TAGCACTCAACTAAAATGCT+AGG | - | chr5.2:14516467-14516486 | None:intergenic | 35.0% | |
| ! | TTTTTACACTTACCGGAAGT+TGG | - | chr5.2:14515249-14515268 | None:intergenic | 35.0% |
| !! | GCTAATGTTATTGCTTTTGC+AGG | + | chr5.2:14516219-14516238 | MS.gene063555:intron | 35.0% |
| !!! | TAGCATTTTAGTTGAGTGCT+AGG | + | chr5.2:14516466-14516485 | MS.gene063555:intron | 35.0% |
| !!! | TTTTGAGGTCAAAGCTTGTT+TGG | + | chr5.2:14516609-14516628 | MS.gene063555:intron | 35.0% |
| !!! | TTTTTTCAAAGCACGATGTG+TGG | + | chr5.2:14515343-14515362 | MS.gene063555:intron | 35.0% |
| AACATACAAATCTCATGGCG+CGG | - | chr5.2:14516170-14516189 | None:intergenic | 40.0% | |
| AAGCACGATGTGTGGTTTAA+AGG | + | chr5.2:14515351-14515370 | MS.gene063555:intron | 40.0% | |
| ACCTGTTTATTGTGATGTGC+AGG | + | chr5.2:14515731-14515750 | MS.gene063555:intron | 40.0% | |
| AGCACGATGTGTGGTTTAAA+GGG | + | chr5.2:14515352-14515371 | MS.gene063555:intron | 40.0% | |
| ATAAGGTATGTAATCGCACC+AGG | - | chr5.2:14515787-14515806 | None:intergenic | 40.0% | |
| CTCAAACAAGAAGTGAAAGG+GGG | - | chr5.2:14516438-14516457 | None:intergenic | 40.0% | |
| GTGTGGTTTAAAGGGGAATT+TGG | + | chr5.2:14515360-14515379 | MS.gene063555:CDS | 40.0% | |
| TCCTGCACATCACAATAAAC+AGG | - | chr5.2:14515735-14515754 | None:intergenic | 40.0% | |
| TCTAGCTGGCCATAAGTATT+TGG | + | chr5.2:14515438-14515457 | MS.gene063555:CDS | 40.0% | |
| TGGTTTAAAGGGGAATTTGG+AGG | + | chr5.2:14515363-14515382 | MS.gene063555:CDS | 40.0% | |
| ! | AACCAAAGTGTTCCAACTTC+CGG | + | chr5.2:14515234-14515253 | MS.gene063555:CDS | 40.0% |
| ! | GTATGGAAAGTCGTGAGTTT+TGG | - | chr5.2:14515713-14515732 | None:intergenic | 40.0% |
| ! | TATGCTGCTTGGACTCAAAA+TGG | + | chr5.2:14516689-14516708 | MS.gene063555:CDS | 40.0% |
| ACCACGGAAGAGTTTAATGC+TGG | + | chr5.2:14515754-14515773 | MS.gene063555:CDS | 45.0% | |
| AGGACGTGAATGAAAGCAAG+CGG | - | chr5.2:14514987-14515006 | None:intergenic | 45.0% | |
| GCACGATGTGTGGTTTAAAG+GGG | + | chr5.2:14515353-14515372 | MS.gene063555:intron | 45.0% | |
| GCTTGGGAAAAGGTCTATGA+TGG | + | chr5.2:14516249-14516268 | MS.gene063555:CDS | 45.0% | |
| GGGAATTTGGAGGAAGTTGT+TGG | + | chr5.2:14515373-14515392 | MS.gene063555:CDS | 45.0% | |
| GTGTTCTGCCGTAGAATTAG+AGG | - | chr5.2:14515007-14515026 | None:intergenic | 45.0% | |
| TACCGGAAGTTGGAACACTT+TGG | - | chr5.2:14515239-14515258 | None:intergenic | 45.0% | |
| TATTGTGATGTGCAGGACCA+CGG | + | chr5.2:14515738-14515757 | MS.gene063555:intron | 45.0% | |
| TCCAGCATTAAACTCTTCCG+TGG | - | chr5.2:14515758-14515777 | None:intergenic | 45.0% | |
| TGCATTGTATAGGGGTTCAC+CGG | + | chr5.2:14516644-14516663 | MS.gene063555:intron | 45.0% | |
| TGGACTGAAAGTCGAGTACA+CGG | + | chr5.2:14515579-14515598 | MS.gene063555:intron | 45.0% | |
| ! | TTTTGCAGGGTTGTCAGCTT+GGG | + | chr5.2:14516233-14516252 | MS.gene063555:intron | 45.0% |
| !!! | TATGTAATTTTTAAATTTAT+TGG | + | chr5.2:14515559-14515578 | MS.gene063555:intron | 5.0% |
| ACCAGCTATGTCTGTGAGTC+CGG | - | chr5.2:14516666-14516685 | None:intergenic | 50.0% | |
| ACGTGAATGAAAGCAAGCGG+CGG | - | chr5.2:14514984-14515003 | None:intergenic | 50.0% | |
| AGGGTTGTCAGCTTGGGAAA+AGG | + | chr5.2:14516239-14516258 | MS.gene063555:intron | 50.0% | |
| GGCAGAACACGACCATAACA+AGG | + | chr5.2:14515017-14515036 | MS.gene063555:CDS | 50.0% | |
| TAGCTGGTGGTTATGCTGCT+TGG | + | chr5.2:14516678-14516697 | MS.gene063555:CDS | 50.0% | |
| TTTAATGCTGGACACGCTCC+TGG | + | chr5.2:14515766-14515785 | MS.gene063555:CDS | 50.0% | |
| ! | CTTTTGCAGGGTTGTCAGCT+TGG | + | chr5.2:14516232-14516251 | MS.gene063555:intron | 50.0% |
| ACCGGACTCACAGACATAGC+TGG | + | chr5.2:14516662-14516681 | MS.gene063555:CDS | 55.0% | |
| GCTCACGAGCTTCTTCTAGC+TGG | + | chr5.2:14515424-14515443 | MS.gene063555:CDS | 55.0% | |
| GGACTCACAGACATAGCTGG+TGG | + | chr5.2:14516665-14516684 | MS.gene063555:CDS | 55.0% | |
| ! | ACACGCACTGGCACTGAAGT+TGG | - | chr5.2:14515403-14515422 | None:intergenic | 55.0% |
| AGCTCGTGAGCTACACGCAC+TGG | - | chr5.2:14515415-14515434 | None:intergenic | 60.0% | |
| CAAATCTCATGGCGCGGCGA+AGG | - | chr5.2:14516164-14516183 | None:intergenic | 60.0% | |
| GCAAGCGGCGGAGACATCAA+AGG | - | chr5.2:14514972-14514991 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 14514963 | 14516730 | 14514963 | ID=MS.gene063555 |
| chr5.2 | mRNA | 14514963 | 14516730 | 14514963 | ID=MS.gene063555.t1;Parent=MS.gene063555 |
| chr5.2 | exon | 14514963 | 14515038 | 14514963 | ID=MS.gene063555.t1.exon1;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14514963 | 14515038 | 14514963 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14515178 | 14515255 | 14515178 | ID=MS.gene063555.t1.exon2;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14515178 | 14515255 | 14515178 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14515354 | 14515467 | 14515354 | ID=MS.gene063555.t1.exon3;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14515354 | 14515467 | 14515354 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14515753 | 14515808 | 14515753 | ID=MS.gene063555.t1.exon4;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14515753 | 14515808 | 14515753 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14516078 | 14516148 | 14516078 | ID=MS.gene063555.t1.exon5;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14516078 | 14516148 | 14516078 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14516241 | 14516294 | 14516241 | ID=MS.gene063555.t1.exon6;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14516241 | 14516294 | 14516241 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| chr5.2 | exon | 14516656 | 14516730 | 14516656 | ID=MS.gene063555.t1.exon7;Parent=MS.gene063555.t1 |
| chr5.2 | CDS | 14516656 | 14516730 | 14516656 | ID=cds.MS.gene063555.t1;Parent=MS.gene063555.t1 |
| Gene Sequence |
| Protein sequence |
