AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050404


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050404.t1 MTR_7g109520 96.094 128 4 1 1 127 1 128 6.92e-88 251
MS.gene050404.t1 MTR_1g087030 64.615 130 41 3 2 127 3 131 6.47e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050404.t1 AT3G62410 58.647 133 47 5 1 127 1 131 1.48e-45 144
MS.gene050404.t1 AT2G47400 68.041 97 27 2 33 127 30 124 4.72e-40 130
MS.gene050404.t1 AT1G76560 47.297 74 37 1 56 127 61 134 6.66e-19 77.0

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTGATATCTGTTC+TGG 0.263996 7.2:-5710178 None:intergenic
TGTTGTTGGTGAGATCTTGA+TGG 0.363034 7.2:-5710086 None:intergenic
GCTACGCATTCTCCACTAAC+CGG 0.387490 7.2:-5710244 None:intergenic
AAGCTTCTCTTCAACTCATC+AGG 0.395970 7.2:+5710052 MS.gene050404:CDS
GATGAGTTGAAGAGAAGCTT+GGG 0.422285 7.2:-5710049 None:intergenic
TGTTCTGGTGCAGCACGAAC+TGG 0.440452 7.2:-5710163 None:intergenic
CACCATCATTCGACCTGATC+CGG 0.449625 7.2:-5710134 None:intergenic
CGGTGGGGTGTAGCCGGATC+AGG 0.450264 7.2:+5710121 MS.gene050404:CDS
CGTGTCTGCAGTGGTGTTGT+TGG 0.454736 7.2:-5710100 None:intergenic
GGTGAGATCTTGATGGTTTG+TGG 0.456742 7.2:-5710079 None:intergenic
TGTGCTGATGACCCGGTTAG+TGG 0.494167 7.2:+5710232 MS.gene050404:CDS
GAAGAAGGATTCTGATCCAT+TGG 0.495582 7.2:+5710321 MS.gene050404:CDS
CGGCGAGTCATGCAAGGGAT+AGG 0.497910 7.2:+5710299 MS.gene050404:CDS
TGGAGAATGCGTAGCAGCAT+GGG 0.517025 7.2:+5710252 MS.gene050404:CDS
CAGACACGGTGGGGTGTAGC+CGG 0.517241 7.2:+5710115 MS.gene050404:CDS
TGATGAGTTGAAGAGAAGCT+TGG 0.520626 7.2:-5710050 None:intergenic
AGAGAAGCTTGGGGTTTGAA+AGG 0.525586 7.2:-5710039 None:intergenic
TCCATTGGAGGATTACTGTA+AGG 0.532128 7.2:+5710336 MS.gene050404:CDS
GTGCTGCGGCGAGTCATGCA+AGG 0.540725 7.2:+5710293 MS.gene050404:CDS
GTGGAGAATGCGTAGCAGCA+TGG 0.561567 7.2:+5710251 MS.gene050404:CDS
CGTAGCAGCATGGGATGAAG+TGG 0.567468 7.2:+5710261 MS.gene050404:CDS
TCATGCAAGGGATAGGAAGA+AGG 0.572718 7.2:+5710306 MS.gene050404:CDS
ACCAGAACAGATATCAAAGA+AGG 0.574036 7.2:+5710177 MS.gene050404:CDS
AGAGACATGTGCTGATGACC+CGG 0.581174 7.2:+5710225 MS.gene050404:CDS
TCCTTACAGTAATCCTCCAA+TGG 0.581674 7.2:-5710337 None:intergenic
CTACGCATTCTCCACTAACC+GGG 0.586814 7.2:-5710243 None:intergenic
AGCCGGATCAGGTCGAATGA+TGG 0.587328 7.2:+5710132 MS.gene050404:CDS
CAACAACACCACTGCAGACA+CGG 0.593172 7.2:+5710101 MS.gene050404:CDS
GAAGGATTCTGATCCATTGG+AGG 0.594880 7.2:+5710324 MS.gene050404:CDS
ACACCCCACCGTGTCTGCAG+TGG 0.598200 7.2:-5710109 None:intergenic
AACACCACTGCAGACACGGT+GGG 0.598529 7.2:+5710105 MS.gene050404:CDS
ACACCACTGCAGACACGGTG+GGG 0.612423 7.2:+5710106 MS.gene050404:CDS
CGGATCAGGTCGAATGATGG+TGG 0.612425 7.2:+5710135 MS.gene050404:CDS
AGCAGCATGGGATGAAGTGG+AGG 0.620459 7.2:+5710264 MS.gene050404:CDS
TGCTGCGGCGAGTCATGCAA+GGG 0.625380 7.2:+5710294 MS.gene050404:CDS
CAACACCACTGCAGACACGG+TGG 0.673443 7.2:+5710104 MS.gene050404:CDS
ATGAGTTGAAGAGAAGCTTG+GGG 0.682917 7.2:-5710048 None:intergenic
AGTGGAGGAACTCAGTGCTG+CGG 0.688380 7.2:+5710279 MS.gene050404:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACCAGAACAGATATCAAAGA+AGG + chr7.2:5710177-5710196 MS.gene050404:CDS 35.0%
ACCTTCTTTGATATCTGTTC+TGG - chr7.2:5710181-5710200 None:intergenic 35.0%
AAGCTTCTCTTCAACTCATC+AGG + chr7.2:5710052-5710071 MS.gene050404:CDS 40.0%
GAAGAAGGATTCTGATCCAT+TGG + chr7.2:5710321-5710340 MS.gene050404:CDS 40.0%
TCCATTGGAGGATTACTGTA+AGG + chr7.2:5710336-5710355 MS.gene050404:CDS 40.0%
TCCTTACAGTAATCCTCCAA+TGG - chr7.2:5710340-5710359 None:intergenic 40.0%
! ATGAGTTGAAGAGAAGCTTG+GGG - chr7.2:5710051-5710070 None:intergenic 40.0%
! GATGAGTTGAAGAGAAGCTT+GGG - chr7.2:5710052-5710071 None:intergenic 40.0%
! TGATGAGTTGAAGAGAAGCT+TGG - chr7.2:5710053-5710072 None:intergenic 40.0%
! TGTTGTTGGTGAGATCTTGA+TGG - chr7.2:5710089-5710108 None:intergenic 40.0%
! TTTTGCACTCATCAGTCTCA+GGG - chr7.2:5710366-5710385 None:intergenic 40.0%
AGAGAAGCTTGGGGTTTGAA+AGG - chr7.2:5710042-5710061 None:intergenic 45.0%
GAAGGATTCTGATCCATTGG+AGG + chr7.2:5710324-5710343 MS.gene050404:CDS 45.0%
TCATGCAAGGGATAGGAAGA+AGG + chr7.2:5710306-5710325 MS.gene050404:CDS 45.0%
! GGTGAGATCTTGATGGTTTG+TGG - chr7.2:5710082-5710101 None:intergenic 45.0%
! GTTTTGCACTCATCAGTCTC+AGG - chr7.2:5710367-5710386 None:intergenic 45.0%
CAACAACACCACTGCAGACA+CGG + chr7.2:5710101-5710120 MS.gene050404:CDS 50.0%
CACCATCATTCGACCTGATC+CGG - chr7.2:5710137-5710156 None:intergenic 50.0%
CTACGCATTCTCCACTAACC+GGG - chr7.2:5710246-5710265 None:intergenic 50.0%
GCTACGCATTCTCCACTAAC+CGG - chr7.2:5710247-5710266 None:intergenic 50.0%
TGGAGAATGCGTAGCAGCAT+GGG + chr7.2:5710252-5710271 MS.gene050404:CDS 50.0%
! AGAGACATGTGCTGATGACC+CGG + chr7.2:5710225-5710244 MS.gene050404:CDS 50.0%
AACACCACTGCAGACACGGT+GGG + chr7.2:5710105-5710124 MS.gene050404:CDS 55.0%
AGCAGCATGGGATGAAGTGG+AGG + chr7.2:5710264-5710283 MS.gene050404:CDS 55.0%
AGCCGGATCAGGTCGAATGA+TGG + chr7.2:5710132-5710151 MS.gene050404:CDS 55.0%
AGTGGAGGAACTCAGTGCTG+CGG + chr7.2:5710279-5710298 MS.gene050404:CDS 55.0%
CGGATCAGGTCGAATGATGG+TGG + chr7.2:5710135-5710154 MS.gene050404:CDS 55.0%
CGTAGCAGCATGGGATGAAG+TGG + chr7.2:5710261-5710280 MS.gene050404:CDS 55.0%
GTGGAGAATGCGTAGCAGCA+TGG + chr7.2:5710251-5710270 MS.gene050404:CDS 55.0%
TGTTCTGGTGCAGCACGAAC+TGG - chr7.2:5710166-5710185 None:intergenic 55.0%
! TGTGCTGATGACCCGGTTAG+TGG + chr7.2:5710232-5710251 MS.gene050404:CDS 55.0%
!! CGTGTCTGCAGTGGTGTTGT+TGG - chr7.2:5710103-5710122 None:intergenic 55.0%
ACACCACTGCAGACACGGTG+GGG + chr7.2:5710106-5710125 MS.gene050404:CDS 60.0%
CAACACCACTGCAGACACGG+TGG + chr7.2:5710104-5710123 MS.gene050404:CDS 60.0%
CGGCGAGTCATGCAAGGGAT+AGG + chr7.2:5710299-5710318 MS.gene050404:CDS 60.0%
TGCTGCGGCGAGTCATGCAA+GGG + chr7.2:5710294-5710313 MS.gene050404:CDS 60.0%
ACACCCCACCGTGTCTGCAG+TGG - chr7.2:5710112-5710131 None:intergenic 65.0%
CAGACACGGTGGGGTGTAGC+CGG + chr7.2:5710115-5710134 MS.gene050404:CDS 65.0%
GTGCTGCGGCGAGTCATGCA+AGG + chr7.2:5710293-5710312 MS.gene050404:CDS 65.0%
CGGTGGGGTGTAGCCGGATC+AGG + chr7.2:5710121-5710140 MS.gene050404:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 5710016 5710399 5710016 ID=MS.gene050404
chr7.2 mRNA 5710016 5710399 5710016 ID=MS.gene050404.t1;Parent=MS.gene050404
chr7.2 exon 5710016 5710399 5710016 ID=MS.gene050404.t1.exon1;Parent=MS.gene050404.t1
chr7.2 CDS 5710016 5710399 5710016 ID=cds.MS.gene050404.t1;Parent=MS.gene050404.t1
Gene Sequence

>MS.gene050404

ATGGCAACAATAGGTGGTCTCAGCCTTTCAAACCCCAAGCTTCTCTTCAACTCATCAGGATTTCCACAAACCATCAAGATCTCACCAACAACACCACTGCAGACACGGTGGGGTGTAGCCGGATCAGGTCGAATGATGGTGGTTCGACCAGTTCGTGCTGCACCAGAACAGATATCAAAGAAGGTAGAAGAAAGCATTAAGAGTGCGCAAGAGACATGTGCTGATGACCCGGTTAGTGGAGAATGCGTAGCAGCATGGGATGAAGTGGAGGAACTCAGTGCTGCGGCGAGTCATGCAAGGGATAGGAAGAAGGATTCTGATCCATTGGAGGATTACTGTAAGGATAACCCTGAGACTGATGAGTGCAAAACTTATGATAACTGA

Protein sequence

>MS.gene050404.t1

MATIGGLSLSNPKLLFNSSGFPQTIKISPTTPLQTRWGVAGSGRMMVVRPVRAAPEQISKKVEESIKSAQETCADDPVSGECVAAWDEVEELSAAASHARDRKKDSDPLEDYCKDNPETDECKTYDN