Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058748.t1 | XP_003597997.1 | 100 | 279 | 0 | 0 | 1 | 279 | 77 | 355 | 1.60E-148 | 535.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058748.t1 | Q8L4R0 | 78.9 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 1.3e-122 | 440.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058748.t1 | G7IYB6 | 100.0 | 279 | 0 | 0 | 1 | 279 | 77 | 355 | 1.1e-148 | 535.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050507 | MS.gene058748 | 0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene058748 | 0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene058748 | 0.814626 | 1.40E-51 | -1.69E-46 |
| MS.gene051617 | MS.gene058748 | 0.832889 | 7.31E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene058748 | 0.813545 | 2.42E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene058748 | 0.80178 | 7.66E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene058748 | 0.801855 | 7.40E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene058748 | 0.814643 | 1.39E-51 | -1.69E-46 |
| MS.gene05602 | MS.gene058748 | 0.802919 | 4.46E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene058748 | 0.818626 | 1.78E-52 | -1.69E-46 |
| MS.gene056897 | MS.gene058748 | 0.825647 | 4.18E-54 | -1.69E-46 |
| MS.gene056903 | MS.gene058748 | 0.800463 | 1.42E-48 | -1.69E-46 |
| MS.gene056947 | MS.gene058748 | 0.802674 | 5.01E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene058748 | 0.874809 | 5.05E-68 | -1.69E-46 |
| MS.gene058293 | MS.gene058748 | 0.800442 | 1.44E-48 | -1.69E-46 |
| MS.gene058365 | MS.gene058748 | 0.812285 | 4.58E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene058787 | 0.818387 | 2.01E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene058948 | 0.805767 | 1.14E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene059692 | 0.807183 | 5.72E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene059695 | 0.807081 | 6.02E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene061606 | 0.819105 | 1.38E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene062145 | 0.80208 | 6.65E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene062891 | 0.808041 | 3.76E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene063555 | 0.810682 | 1.02E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene063560 | 0.81745 | 3.28E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene065032 | 0.812822 | 3.49E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene065680 | 0.803652 | 3.15E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene065913 | 0.824317 | 8.62E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene06688 | 0.807263 | 5.51E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene067002 | 0.832319 | 1.01E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene067414 | 0.817783 | 2.76E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene068240 | 0.819735 | 9.94E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene06897 | 0.811651 | 6.29E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene069302 | 0.816819 | 4.54E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene070266 | 0.811411 | 7.10E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene071685 | 0.810296 | 1.24E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene073077 | 0.806867 | 6.68E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene07311 | 0.809366 | 1.96E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene07312 | 0.803162 | 3.98E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene07315 | 0.801393 | 9.20E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene073295 | 0.811594 | 6.48E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene073806 | 0.813042 | 3.13E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene073925 | 0.81637 | 5.73E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene073927 | 0.84682 | 1.71E-59 | -1.69E-46 |
| MS.gene058748 | MS.gene09953 | 0.82043 | 6.89E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene20931 | 0.821713 | 3.49E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene21635 | 0.802231 | 6.19E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene21953 | 0.801047 | 1.08E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene22291 | 0.815553 | 8.72E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene23357 | 0.802603 | 5.19E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene25693 | 0.803473 | 3.43E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene27002 | 0.811988 | 5.31E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene27264 | 0.828087 | 1.09E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene28163 | 0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene28526 | 0.815724 | 7.98E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene28847 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene32990 | 0.830292 | 3.19E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene34231 | 0.813115 | 3.01E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene34294 | 0.829211 | 5.84E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene34296 | 0.822092 | 2.85E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene38554 | 0.806984 | 6.31E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene39446 | 0.83436 | 3.14E-56 | -1.69E-46 |
| MS.gene058748 | MS.gene39565 | 0.800744 | 1.25E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene39909 | 0.83759 | 4.75E-57 | -1.69E-46 |
| MS.gene058748 | MS.gene39910 | 0.826141 | 3.19E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene40023 | 0.842144 | 3.09E-58 | -1.69E-46 |
| MS.gene058748 | MS.gene40511 | 0.837844 | 4.09E-57 | -1.69E-46 |
| MS.gene058748 | MS.gene40589 | 0.813833 | 2.09E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene46340 | 0.820366 | 7.13E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene46645 | 0.820379 | 7.08E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene49205 | 0.811922 | 5.49E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene50731 | 0.81012 | 1.35E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene50826 | 0.925564 | 1.55E-90 | -1.69E-46 |
| MS.gene058748 | MS.gene50857 | 0.963192 | 8.73E-122 | -1.69E-46 |
| MS.gene058748 | MS.gene52786 | 0.80618 | 9.32E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene54080 | 0.829029 | 6.47E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene55937 | 0.818452 | 1.95E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene56204 | 0.811811 | 5.81E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene56599 | 0.813728 | 2.21E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene57232 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene62506 | 0.800804 | 1.21E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene62624 | 0.81802 | 2.44E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene63489 | 0.807661 | 4.53E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene67598 | 0.800116 | 1.68E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene68129 | 0.800612 | 1.33E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene68130 | 0.806215 | 9.17E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene68242 | 0.803639 | 3.17E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene68423 | 0.81317 | 2.93E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene68729 | 0.805809 | 1.12E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene69810 | 0.811179 | 7.97E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene70304 | 0.813844 | 2.08E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene70352 | 0.811534 | 6.67E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene70467 | 0.830364 | 3.06E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene71692 | 0.817642 | 2.97E-52 | -1.69E-46 |
| MS.gene058748 | MS.gene74946 | 0.829305 | 5.55E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene75297 | 0.82398 | 1.03E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene75493 | 0.811981 | 5.33E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene76198 | 0.801143 | 1.03E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene76520 | 0.822827 | 1.92E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene77868 | 0.827543 | 1.48E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene78852 | 0.815156 | 1.07E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene82036 | 0.813209 | 2.87E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene83543 | 0.839554 | 1.48E-57 | -1.69E-46 |
| MS.gene058748 | MS.gene83615 | 0.804007 | 2.66E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene84154 | 0.807219 | 5.62E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene84371 | 0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene84483 | 0.800452 | 1.43E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene85135 | 0.804506 | 2.09E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene85478 | 0.822414 | 2.40E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene86306 | 0.836981 | 6.80E-57 | -1.69E-46 |
| MS.gene058748 | MS.gene86413 | 0.809793 | 1.59E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene87369 | 0.807627 | 4.61E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene88851 | 0.811071 | 8.41E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene91827 | 0.80688 | 6.64E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene92294 | 0.806474 | 8.08E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene92299 | 0.808875 | 2.50E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene92452 | 0.853332 | 2.56E-61 | -1.69E-46 |
| MS.gene058748 | MS.gene92659 | 0.842803 | 2.07E-58 | -1.69E-46 |
| MS.gene058748 | MS.gene92661 | 0.8378 | 4.20E-57 | -1.69E-46 |
| MS.gene058748 | MS.gene92714 | 0.82892 | 6.88E-55 | -1.69E-46 |
| MS.gene058748 | MS.gene94292 | 0.806756 | 7.05E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene94547 | 0.807896 | 4.04E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene95988 | 0.802284 | 6.03E-49 | -1.69E-46 |
| MS.gene058748 | MS.gene97266 | 0.806989 | 6.29E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene98137 | 0.824291 | 8.74E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene98170 | 0.806115 | 9.62E-50 | -1.69E-46 |
| MS.gene058748 | MS.gene99936 | 0.804812 | 1.80E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058748.t1 | MTR_3g005100 | 100.000 | 279 | 0 | 0 | 1 | 279 | 77 | 355 | 0.0 | 559 |
| MS.gene058748.t1 | MTR_3g008040 | 83.513 | 279 | 15 | 2 | 1 | 279 | 293 | 540 | 1.58e-155 | 444 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
| MS.gene058748.t1 | AT1G19800 | 78.929 | 280 | 58 | 1 | 1 | 279 | 71 | 350 | 9.80e-165 | 461 |
Find 76 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAACAAATTGACACATTA+AGG | 0.141685 | 3.1:+420859 | MS.gene058748:CDS |
| AGACATACTCCAACCGATTT+TGG | 0.154799 | 3.1:-420639 | None:intergenic |
| CACCCATGTTTCTCAGTAAT+TGG | 0.245755 | 3.1:+420493 | MS.gene058748:CDS |
| CTACTCGCCGATGGTGTTTA+TGG | 0.268277 | 3.1:+420996 | MS.gene058748:CDS |
| ATTGACACATTAAGGGTTCT+TGG | 0.272812 | 3.1:+420867 | MS.gene058748:CDS |
| AATTAGCATTAACATTATAT+TGG | 0.273273 | 3.1:+421019 | MS.gene058748:CDS |
| TGTTGTTTCTTCCAAGGAGC+TGG | 0.299987 | 3.1:+421242 | MS.gene058748:CDS |
| GCTGTTGTGATTTCTCTTGT+TGG | 0.309665 | 3.1:+421191 | MS.gene058748:CDS |
| ATCAGACCCTGTTGATTATC+TGG | 0.348729 | 3.1:+420890 | MS.gene058748:CDS |
| ATCTATTGTGAGCTGTGCTT+GGG | 0.352418 | 3.1:+421118 | MS.gene058748:CDS |
| ACCCTAATGTAAAACACAAT+AGG | 0.377031 | 3.1:-420959 | None:intergenic |
| CTTTCTTGTTGTTTCTTCCA+AGG | 0.385382 | 3.1:+421236 | MS.gene058748:CDS |
| TGAACAAATTGACACATTAA+GGG | 0.396891 | 3.1:+420860 | MS.gene058748:CDS |
| AACATCTGCCTTTGTTGGGA+TGG | 0.400841 | 3.1:+420665 | MS.gene058748:CDS |
| CCCTAATGTAAAACACAATA+GGG | 0.407783 | 3.1:-420958 | None:intergenic |
| GTTGTTTCTTCCAAGGAGCT+GGG | 0.411014 | 3.1:+421243 | MS.gene058748:CDS |
| CCCATGCTCTGAGAGATCGC+TGG | 0.424886 | 3.1:-421049 | None:intergenic |
| TGAAAGAGTTGGTCCAAAAT+CGG | 0.433002 | 3.1:+420626 | MS.gene058748:CDS |
| GTGAGGGAGTTCACTAGGTT+AGG | 0.450674 | 3.1:+420705 | MS.gene058748:CDS |
| TTAGCTGATCCCCAGCTCCT+TGG | 0.451856 | 3.1:-421253 | None:intergenic |
| GGTGTCACCAGATAATCAAC+AGG | 0.454755 | 3.1:-420897 | None:intergenic |
| ATTAGTGCAATGATCAAGTC+GGG | 0.456623 | 3.1:+421077 | MS.gene058748:CDS |
| ATAGTTGTCGCCGGCCGCAT+TGG | 0.476102 | 3.1:+420798 | MS.gene058748:CDS |
| AACATGGGTGATATAACATT+AGG | 0.482042 | 3.1:-420480 | None:intergenic |
| TATCTATTGTGAGCTGTGCT+TGG | 0.485394 | 3.1:+421117 | MS.gene058748:CDS |
| TTTAGCTCTGGCTTTCTCTA+GGG | 0.488633 | 3.1:+420752 | MS.gene058748:CDS |
| TGTGTTCATTTCGTCATCCA+TGG | 0.509136 | 3.1:-420448 | None:intergenic |
| ATGGGAGGTGCTAAAGGTGT+TGG | 0.515914 | 3.1:+421152 | MS.gene058748:CDS |
| TCATAATGACCTGCCCTGCT+AGG | 0.516210 | 3.1:-420557 | None:intergenic |
| TAAAGGGAAAGGTTCATTGG+AGG | 0.517631 | 3.1:+420586 | MS.gene058748:CDS |
| ACAACAATGGGAGGTGCTAA+AGG | 0.520391 | 3.1:+421146 | MS.gene058748:CDS |
| ATAAACACCATCGGCGAGTA+GGG | 0.522796 | 3.1:-420994 | None:intergenic |
| AATTTGTGAGGGAGTTCACT+AGG | 0.527620 | 3.1:+420700 | MS.gene058748:CDS |
| GCTTGGGGAGTTACAACAAT+GGG | 0.530997 | 3.1:+421134 | MS.gene058748:CDS |
| CCCCTCCATAGGTACCTTGG+CGG | 0.532953 | 3.1:-420519 | None:intergenic |
| CATAAACACCATCGGCGAGT+AGG | 0.533928 | 3.1:-420995 | None:intergenic |
| CTTCAACAACTTGAAAGAGT+TGG | 0.534939 | 3.1:+420615 | MS.gene058748:CDS |
| CCCAGCGATCTCTCAGAGCA+TGG | 0.539097 | 3.1:+421048 | MS.gene058748:CDS |
| CAATAGGGTCAAAAGAGGTA+AGG | 0.541445 | 3.1:-420943 | None:intergenic |
| CCATGCTCTGAGAGATCGCT+GGG | 0.544193 | 3.1:-421048 | None:intergenic |
| GGTCACCGCCAAGGTACCTA+TGG | 0.544354 | 3.1:+420514 | MS.gene058748:CDS |
| ATACAAGAAGCAATCACTCT+TGG | 0.547748 | 3.1:-420918 | None:intergenic |
| AAAAGCACTTCCAATGCGGC+CGG | 0.554228 | 3.1:-420808 | None:intergenic |
| AGCAGAACTAGGAACTATGC+AGG | 0.556768 | 3.1:+420833 | MS.gene058748:CDS |
| TTGTTTCTTCCAAGGAGCTG+GGG | 0.560587 | 3.1:+421244 | MS.gene058748:CDS |
| GTGATATAACATTAGGAGCA+TGG | 0.565838 | 3.1:-420473 | None:intergenic |
| TATTAGTGCAATGATCAAGT+CGG | 0.569938 | 3.1:+421076 | MS.gene058748:CDS |
| AGAGTTGGTCCAAAATCGGT+TGG | 0.570333 | 3.1:+420630 | MS.gene058748:CDS |
| TCTAGTGCCCTACTCGCCGA+TGG | 0.571085 | 3.1:+420987 | MS.gene058748:CDS |
| CTCTAGGGAACTAAGTCCTG+TGG | 0.581058 | 3.1:+420767 | MS.gene058748:CDS |
| TCAGTAATTGGTCACCGCCA+AGG | 0.581319 | 3.1:+420505 | MS.gene058748:CDS |
| TTAGGATTGAATAGATCTGT+AGG | 0.581694 | 3.1:+420723 | MS.gene058748:CDS |
| CACCGCCAAGGTACCTATGG+AGG | 0.589168 | 3.1:+420517 | MS.gene058748:CDS |
| AATCCCCTCCATAGGTACCT+TGG | 0.590656 | 3.1:-420522 | None:intergenic |
| AAACACAATAGGGTCAAAAG+AGG | 0.591740 | 3.1:-420948 | None:intergenic |
| ACAACTATTGCTGTAACCAC+AGG | 0.596084 | 3.1:-420783 | None:intergenic |
| TCTATTGTGAGCTGTGCTTG+GGG | 0.598712 | 3.1:+421119 | MS.gene058748:CDS |
| CCAGCGATCTCTCAGAGCAT+GGG | 0.600835 | 3.1:+421049 | MS.gene058748:CDS |
| TGACCAATTACTGAGAAACA+TGG | 0.602907 | 3.1:-420496 | None:intergenic |
| ACCGCCAAGGTACCTATGGA+GGG | 0.603553 | 3.1:+420518 | MS.gene058748:CDS |
| AAACTGACAATCCCCTCCAT+AGG | 0.606171 | 3.1:-420530 | None:intergenic |
| CCTTCACGATACAATTTGTG+AGG | 0.612348 | 3.1:+420688 | MS.gene058748:CDS |
| CGTGAAGGCCATCCCAACAA+AGG | 0.621760 | 3.1:-420673 | None:intergenic |
| GTTACAGCAATAGTTGTCGC+CGG | 0.627571 | 3.1:+420789 | MS.gene058748:CDS |
| CTTCACGATACAATTTGTGA+GGG | 0.629039 | 3.1:+420689 | MS.gene058748:CDS |
| CCTCACAAATTGTATCGTGA+AGG | 0.633129 | 3.1:-420688 | None:intergenic |
| GCTAATTCCATAAACACCAT+CGG | 0.644465 | 3.1:-421003 | None:intergenic |
| TAGTGCAATGATCAAGTCGG+GGG | 0.657207 | 3.1:+421079 | MS.gene058748:CDS |
| TGGGGAGTTACAACAATGGG+AGG | 0.664113 | 3.1:+421137 | MS.gene058748:CDS |
| GACCAATTACTGAGAAACAT+GGG | 0.666778 | 3.1:-420495 | None:intergenic |
| TTAGTGCAATGATCAAGTCG+GGG | 0.670713 | 3.1:+421078 | MS.gene058748:CDS |
| TGCTTGGGGAGTTACAACAA+TGG | 0.676051 | 3.1:+421133 | MS.gene058748:CDS |
| CCGCCAAGGTACCTATGGAG+GGG | 0.683323 | 3.1:+420519 | MS.gene058748:CDS |
| CTGCAAAAGCACTTCCAATG+CGG | 0.727646 | 3.1:-420812 | None:intergenic |
| GTGTCACCAGATAATCAACA+GGG | 0.735529 | 3.1:-420896 | None:intergenic |
| GGATTGAATAGATCTGTAGG+CGG | 0.738440 | 3.1:+420726 | MS.gene058748:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAGCATTAACATTATAT+TGG | + | chr3.1:421019-421038 | MS.gene058748:CDS | 15.0% |
| !!! | GTCATTATGAAGATTTTAAA+GGG | + | chr3.1:420570-420589 | MS.gene058748:CDS | 20.0% |
| ! | TGAACAAATTGACACATTAA+GGG | + | chr3.1:420860-420879 | MS.gene058748:CDS | 25.0% |
| !! | GGTCATTATGAAGATTTTAA+AGG | + | chr3.1:420569-420588 | MS.gene058748:CDS | 25.0% |
| !!! | TATGAAGATTTTAAAGGGAA+AGG | + | chr3.1:420575-420594 | MS.gene058748:CDS | 25.0% |
| AACATGGGTGATATAACATT+AGG | - | chr3.1:420483-420502 | None:intergenic | 30.0% | |
| ACCCTAATGTAAAACACAAT+AGG | - | chr3.1:420962-420981 | None:intergenic | 30.0% | |
| CCCTAATGTAAAACACAATA+GGG | - | chr3.1:420961-420980 | None:intergenic | 30.0% | |
| CTGAACAAATTGACACATTA+AGG | + | chr3.1:420859-420878 | MS.gene058748:CDS | 30.0% | |
| TATTAGTGCAATGATCAAGT+CGG | + | chr3.1:421076-421095 | MS.gene058748:CDS | 30.0% | |
| TTAGGATTGAATAGATCTGT+AGG | + | chr3.1:420723-420742 | MS.gene058748:CDS | 30.0% | |
| ! | ACCCTATTGTGTTTTACATT+AGG | + | chr3.1:420957-420976 | MS.gene058748:CDS | 30.0% |
| ! | ATTGTGTTTTACATTAGGGT+TGG | + | chr3.1:420962-420981 | MS.gene058748:CDS | 30.0% |
| ! | CCCTATTGTGTTTTACATTA+GGG | + | chr3.1:420958-420977 | MS.gene058748:CDS | 30.0% |
| ! | TTTTAACATCTGCCTTTGTT+GGG | + | chr3.1:420661-420680 | MS.gene058748:CDS | 30.0% |
| !! | TTTTAAAGGGAAAGGTTCAT+TGG | + | chr3.1:420583-420602 | MS.gene058748:CDS | 30.0% |
| AAACACAATAGGGTCAAAAG+AGG | - | chr3.1:420951-420970 | None:intergenic | 35.0% | |
| ATACAAGAAGCAATCACTCT+TGG | - | chr3.1:420921-420940 | None:intergenic | 35.0% | |
| ATTAGTGCAATGATCAAGTC+GGG | + | chr3.1:421077-421096 | MS.gene058748:CDS | 35.0% | |
| ATTGACACATTAAGGGTTCT+TGG | + | chr3.1:420867-420886 | MS.gene058748:CDS | 35.0% | |
| CTTCAACAACTTGAAAGAGT+TGG | + | chr3.1:420615-420634 | MS.gene058748:CDS | 35.0% | |
| CTTCACGATACAATTTGTGA+GGG | + | chr3.1:420689-420708 | MS.gene058748:CDS | 35.0% | |
| GACCAATTACTGAGAAACAT+GGG | - | chr3.1:420498-420517 | None:intergenic | 35.0% | |
| GCTAATTCCATAAACACCAT+CGG | - | chr3.1:421006-421025 | None:intergenic | 35.0% | |
| GTGATATAACATTAGGAGCA+TGG | - | chr3.1:420476-420495 | None:intergenic | 35.0% | |
| TGACCAATTACTGAGAAACA+TGG | - | chr3.1:420499-420518 | None:intergenic | 35.0% | |
| ! | CTTTTAACATCTGCCTTTGT+TGG | + | chr3.1:420660-420679 | MS.gene058748:CDS | 35.0% |
| ! | TGAAAGAGTTGGTCCAAAAT+CGG | + | chr3.1:420626-420645 | MS.gene058748:CDS | 35.0% |
| !! | CTTTCTTGTTGTTTCTTCCA+AGG | + | chr3.1:421236-421255 | MS.gene058748:CDS | 35.0% |
| !! | TGTCAGTTTTAATCCTAGCA+GGG | + | chr3.1:420544-420563 | MS.gene058748:CDS | 35.0% |
| !! | TTGTCAGTTTTAATCCTAGC+AGG | + | chr3.1:420543-420562 | MS.gene058748:CDS | 35.0% |
| AATTTGTGAGGGAGTTCACT+AGG | + | chr3.1:420700-420719 | MS.gene058748:CDS | 40.0% | |
| ACAACTATTGCTGTAACCAC+AGG | - | chr3.1:420786-420805 | None:intergenic | 40.0% | |
| ATCTATTGTGAGCTGTGCTT+GGG | + | chr3.1:421118-421137 | MS.gene058748:CDS | 40.0% | |
| CAATAGGGTCAAAAGAGGTA+AGG | - | chr3.1:420946-420965 | None:intergenic | 40.0% | |
| CACCCATGTTTCTCAGTAAT+TGG | + | chr3.1:420493-420512 | MS.gene058748:CDS | 40.0% | |
| CCTCACAAATTGTATCGTGA+AGG | - | chr3.1:420691-420710 | None:intergenic | 40.0% | |
| CCTTCACGATACAATTTGTG+AGG | + | chr3.1:420688-420707 | MS.gene058748:CDS | 40.0% | |
| GCTGTTGTGATTTCTCTTGT+TGG | + | chr3.1:421191-421210 | MS.gene058748:CDS | 40.0% | |
| GGATTGAATAGATCTGTAGG+CGG | + | chr3.1:420726-420745 | MS.gene058748:CDS | 40.0% | |
| GTGTCACCAGATAATCAACA+GGG | - | chr3.1:420899-420918 | None:intergenic | 40.0% | |
| TAAAGGGAAAGGTTCATTGG+AGG | + | chr3.1:420586-420605 | MS.gene058748:CDS | 40.0% | |
| TATCTATTGTGAGCTGTGCT+TGG | + | chr3.1:421117-421136 | MS.gene058748:CDS | 40.0% | |
| TTAGTGCAATGATCAAGTCG+GGG | + | chr3.1:421078-421097 | MS.gene058748:CDS | 40.0% | |
| ! | ATCAGACCCTGTTGATTATC+TGG | + | chr3.1:420890-420909 | MS.gene058748:CDS | 40.0% |
| !! | AGACATACTCCAACCGATTT+TGG | - | chr3.1:420642-420661 | None:intergenic | 40.0% |
| !! | TTTAGCTCTGGCTTTCTCTA+GGG | + | chr3.1:420752-420771 | MS.gene058748:CDS | 40.0% |
| !!! | TTTTAGCTCTGGCTTTCTCT+AGG | + | chr3.1:420751-420770 | MS.gene058748:CDS | 40.0% |
| AAACTGACAATCCCCTCCAT+AGG | - | chr3.1:420533-420552 | None:intergenic | 45.0% | |
| AACATCTGCCTTTGTTGGGA+TGG | + | chr3.1:420665-420684 | MS.gene058748:CDS | 45.0% | |
| AGCAGAACTAGGAACTATGC+AGG | + | chr3.1:420833-420852 | MS.gene058748:CDS | 45.0% | |
| ATAAACACCATCGGCGAGTA+GGG | - | chr3.1:420997-421016 | None:intergenic | 45.0% | |
| GCTTGGGGAGTTACAACAAT+GGG | + | chr3.1:421134-421153 | MS.gene058748:CDS | 45.0% | |
| GTTACAGCAATAGTTGTCGC+CGG | + | chr3.1:420789-420808 | MS.gene058748:CDS | 45.0% | |
| TAGTGCAATGATCAAGTCGG+GGG | + | chr3.1:421079-421098 | MS.gene058748:CDS | 45.0% | |
| TCTATTGTGAGCTGTGCTTG+GGG | + | chr3.1:421119-421138 | MS.gene058748:CDS | 45.0% | |
| TGCTTGGGGAGTTACAACAA+TGG | + | chr3.1:421133-421152 | MS.gene058748:CDS | 45.0% | |
| ! | AGAGTTGGTCCAAAATCGGT+TGG | + | chr3.1:420630-420649 | MS.gene058748:CDS | 45.0% |
| ! | AGTGCTTTTGCAGCAGAACT+AGG | + | chr3.1:420822-420841 | MS.gene058748:CDS | 45.0% |
| ! | GGTGTCACCAGATAATCAAC+AGG | - | chr3.1:420900-420919 | None:intergenic | 45.0% |
| ! | TTGTTTCTTCCAAGGAGCTG+GGG | + | chr3.1:421244-421263 | MS.gene058748:CDS | 45.0% |
| !! | ACAACAATGGGAGGTGCTAA+AGG | + | chr3.1:421146-421165 | MS.gene058748:CDS | 45.0% |
| !! | AGTTTTAATCCTAGCAGGGC+AGG | + | chr3.1:420548-420567 | MS.gene058748:CDS | 45.0% |
| !! | CTGCAAAAGCACTTCCAATG+CGG | - | chr3.1:420815-420834 | None:intergenic | 45.0% |
| !! | GTTGTTTCTTCCAAGGAGCT+GGG | + | chr3.1:421243-421262 | MS.gene058748:CDS | 45.0% |
| !! | TGTTGTTTCTTCCAAGGAGC+TGG | + | chr3.1:421242-421261 | MS.gene058748:CDS | 45.0% |
| AATCCCCTCCATAGGTACCT+TGG | - | chr3.1:420525-420544 | None:intergenic | 50.0% | |
| CATAAACACCATCGGCGAGT+AGG | - | chr3.1:420998-421017 | None:intergenic | 50.0% | |
| CTACTCGCCGATGGTGTTTA+TGG | + | chr3.1:420996-421015 | MS.gene058748:CDS | 50.0% | |
| GTGAGGGAGTTCACTAGGTT+AGG | + | chr3.1:420705-420724 | MS.gene058748:CDS | 50.0% | |
| TCAGTAATTGGTCACCGCCA+AGG | + | chr3.1:420505-420524 | MS.gene058748:CDS | 50.0% | |
| TCATAATGACCTGCCCTGCT+AGG | - | chr3.1:420560-420579 | None:intergenic | 50.0% | |
| TGGGGAGTTACAACAATGGG+AGG | + | chr3.1:421137-421156 | MS.gene058748:CDS | 50.0% | |
| ! | ATGATCAAGTCGGGGGCTTT+TGG | + | chr3.1:421086-421105 | MS.gene058748:CDS | 50.0% |
| ! | CTCTAGGGAACTAAGTCCTG+TGG | + | chr3.1:420767-420786 | MS.gene058748:CDS | 50.0% |
| !! | AAAAGCACTTCCAATGCGGC+CGG | - | chr3.1:420811-420830 | None:intergenic | 50.0% |
| !! | ATGGGAGGTGCTAAAGGTGT+TGG | + | chr3.1:421152-421171 | MS.gene058748:CDS | 50.0% |
| !!! | TGTAGGCGGTGTTTTAGCTC+TGG | + | chr3.1:420740-420759 | MS.gene058748:CDS | 50.0% |
| CCAGCGATCTCTCAGAGCAT+GGG | + | chr3.1:421049-421068 | MS.gene058748:CDS | 55.0% | |
| CGTGAAGGCCATCCCAACAA+AGG | - | chr3.1:420676-420695 | None:intergenic | 55.0% | |
| TTAGCTGATCCCCAGCTCCT+TGG | - | chr3.1:421256-421275 | None:intergenic | 55.0% | |
| ! | ACCGCCAAGGTACCTATGGA+GGG | + | chr3.1:420518-420537 | MS.gene058748:CDS | 55.0% |
| !! | CCATGCTCTGAGAGATCGCT+GGG | - | chr3.1:421051-421070 | None:intergenic | 55.0% |
| ATAGTTGTCGCCGGCCGCAT+TGG | + | chr3.1:420798-420817 | MS.gene058748:CDS | 60.0% | |
| CCCAGCGATCTCTCAGAGCA+TGG | + | chr3.1:421048-421067 | MS.gene058748:CDS | 60.0% | |
| CCCCTCCATAGGTACCTTGG+CGG | - | chr3.1:420522-420541 | None:intergenic | 60.0% | |
| TCTAGTGCCCTACTCGCCGA+TGG | + | chr3.1:420987-421006 | MS.gene058748:CDS | 60.0% | |
| ! | CACCGCCAAGGTACCTATGG+AGG | + | chr3.1:420517-420536 | MS.gene058748:CDS | 60.0% |
| ! | CCGCCAAGGTACCTATGGAG+GGG | + | chr3.1:420519-420538 | MS.gene058748:CDS | 60.0% |
| ! | GGTCACCGCCAAGGTACCTA+TGG | + | chr3.1:420514-420533 | MS.gene058748:CDS | 60.0% |
| !! | CCCATGCTCTGAGAGATCGC+TGG | - | chr3.1:421052-421071 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 420450 | 421289 | 420450 | ID=MS.gene058748 |
| chr3.1 | mRNA | 420450 | 421289 | 420450 | ID=MS.gene058748.t1;Parent=MS.gene058748 |
| chr3.1 | exon | 420450 | 421289 | 420450 | ID=MS.gene058748.t1.exon1;Parent=MS.gene058748.t1 |
| chr3.1 | CDS | 420450 | 421289 | 420450 | ID=cds.MS.gene058748.t1;Parent=MS.gene058748.t1 |
| Gene Sequence |
| Protein sequence |
