AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064122.t1 MTR_4g023030 96.148 623 18 1 1 617 1 623 0.0 1230
MS.gene064122.t1 MTR_3g026650 69.692 617 133 9 1 617 1 563 0.0 801
MS.gene064122.t1 MTR_3g026650 67.652 541 121 9 1 541 1 487 0.0 665
MS.gene064122.t1 MTR_3g026650 63.956 455 110 9 1 455 1 401 4.66e-178 510
MS.gene064122.t1 MTR_4g125460 36.830 429 251 8 193 610 59 478 2.01e-87 280
MS.gene064122.t1 MTR_4g125460 36.818 440 257 9 183 610 187 617 5.70e-86 281
MS.gene064122.t1 MTR_3g096020 36.652 442 243 11 192 610 159 586 5.50e-80 264
MS.gene064122.t1 MTR_5g064620 35.778 450 243 10 190 610 79 511 6.83e-80 262
MS.gene064122.t1 MTR_4g100810 35.654 474 267 12 151 610 40 489 1.96e-77 256
MS.gene064122.t1 MTR_5g099010 39.031 392 222 8 229 613 121 502 3.19e-77 256
MS.gene064122.t1 MTR_3g115220 37.975 395 223 6 229 613 113 495 1.75e-76 254
MS.gene064122.t1 MTR_5g012770 36.211 475 264 12 151 616 56 500 1.71e-75 250
MS.gene064122.t1 MTR_5g081690 35.681 426 254 9 196 610 94 510 1.71e-75 250
MS.gene064122.t1 MTR_3g099480 36.585 451 235 12 190 610 108 537 3.03e-75 250
MS.gene064122.t1 MTR_4g082310 36.409 401 236 8 230 616 100 495 3.73e-75 249
MS.gene064122.t1 MTR_5g032100 34.802 454 245 10 196 610 60 501 2.25e-73 244
MS.gene064122.t1 MTR_4g019210 34.186 430 263 8 196 614 205 625 5.11e-73 247
MS.gene064122.t1 MTR_4g110030 37.903 372 219 6 230 592 119 487 2.08e-71 239
MS.gene064122.t1 MTR_6g016015 37.600 375 218 8 230 593 118 487 3.01e-71 239
MS.gene064122.t1 MTR_3g100310 33.987 459 250 10 192 610 70 515 6.74e-71 238
MS.gene064122.t1 MTR_5g042200 35.802 405 235 9 230 617 88 484 8.77e-71 237
MS.gene064122.t1 MTR_4g084570 33.787 441 256 11 198 616 127 553 5.76e-70 236
MS.gene064122.t1 MTR_2g059350 36.957 368 218 6 230 587 118 481 1.06e-69 235
MS.gene064122.t1 MTR_3g086570 33.034 445 261 12 192 610 114 547 9.68e-69 233
MS.gene064122.t1 MTR_1g031590 34.247 438 248 11 198 611 126 547 1.28e-68 233
MS.gene064122.t1 MTR_5g059260 33.416 404 242 6 230 610 160 559 5.20e-65 224
MS.gene064122.t1 MTR_3g005340 33.333 396 248 6 230 610 150 544 2.48e-63 219
MS.gene064122.t1 MTR_1g102030 30.206 437 274 11 196 613 61 485 1.16e-57 202
MS.gene064122.t1 MTR_4g023050 95.000 80 4 0 538 617 29 108 9.47e-50 168
MS.gene064122.t1 MTR_2g016470 28.842 475 267 16 192 610 155 614 6.86e-49 180
MS.gene064122.t1 MTR_4g132590 30.704 469 263 17 193 610 35 492 5.40e-47 172
MS.gene064122.t1 MTR_4g132590 30.704 469 263 17 193 610 134 591 2.82e-46 172
MS.gene064122.t1 MTR_3g086570 31.288 326 190 10 192 492 114 430 2.70e-44 164
MS.gene064122.t1 MTR_1g101990 31.690 284 182 7 335 613 5 281 6.77e-43 157
MS.gene064122.t1 MTR_4g010990 27.569 399 233 13 230 610 78 438 1.22e-31 128
MS.gene064122.t1 MTR_7g017330 30.000 290 188 6 230 504 101 390 1.62e-30 127
MS.gene064122.t1 MTR_4g010990 26.873 387 227 13 243 611 2 350 4.55e-28 116
MS.gene064122.t1 MTR_3g092590 27.362 307 208 6 213 504 76 382 2.97e-25 110
MS.gene064122.t1 MTR_3g092590 27.362 307 208 6 213 504 76 382 3.62e-25 110
MS.gene064122.t1 MTR_1g088900 56.322 87 8 1 315 401 38 94 9.41e-23 98.2
MS.gene064122.t1 MTR_4g008200 24.734 376 216 12 192 555 18 338 7.00e-21 95.1
MS.gene064122.t1 MTR_2g038320 24.339 378 221 13 207 563 77 410 9.70e-21 95.5
MS.gene064122.t1 MTR_4g008200 24.734 376 216 12 192 555 69 389 1.44e-20 95.1
MS.gene064122.t1 MTR_7g064210 31.100 209 129 4 333 529 19 224 1.53e-20 94.7
MS.gene064122.t1 MTR_1g007390 27.170 265 178 6 255 504 124 388 4.29e-20 95.1
MS.gene064122.t1 MTR_1g101985 39.623 106 60 1 196 301 61 162 1.28e-16 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064122.t1 AT2G44500 63.696 606 157 9 11 612 17 563 0.0 749
MS.gene064122.t1 AT3G07900 60.065 611 192 13 10 615 14 577 0.0 686
MS.gene064122.t1 AT5G63390 60.656 427 167 1 192 617 129 555 0.0 556
MS.gene064122.t1 AT2G44500 57.016 449 130 9 11 455 17 406 6.51e-163 473
MS.gene064122.t1 AT5G35570 36.927 436 243 11 198 616 226 646 5.15e-81 268
MS.gene064122.t1 AT1G76270 36.806 432 240 10 196 613 77 489 8.74e-80 263
MS.gene064122.t1 AT1G20550 36.552 435 260 11 183 613 70 492 5.56e-79 261
MS.gene064122.t1 AT4G24530 35.808 458 241 11 193 610 72 516 1.93e-78 258
MS.gene064122.t1 AT3G54100 34.112 428 266 7 196 614 195 615 3.66e-76 255
MS.gene064122.t1 AT5G64600 35.973 442 246 11 193 611 88 515 7.20e-76 251
MS.gene064122.t1 AT1G22460 36.073 438 243 10 196 610 134 557 1.37e-75 252
MS.gene064122.t1 AT5G01100 34.101 434 266 8 196 617 190 615 7.40e-74 249
MS.gene064122.t1 AT5G65470 33.547 468 258 10 185 611 48 503 1.28e-73 245
MS.gene064122.t1 AT4G38390 37.500 400 219 8 229 613 125 508 4.37e-73 244
MS.gene064122.t1 AT2G37980 33.103 435 261 9 196 614 199 619 4.54e-73 247
MS.gene064122.t1 AT2G03280 38.070 373 215 7 231 592 92 459 8.18e-73 242
MS.gene064122.t1 AT5G15740 36.461 373 223 6 230 592 109 477 5.81e-70 235
MS.gene064122.t1 AT1G14020 35.930 398 234 8 231 613 95 486 1.16e-69 234
MS.gene064122.t1 AT4G16650 37.813 439 245 13 185 611 82 504 3.20e-68 232
MS.gene064122.t1 AT4G16650 37.813 439 245 13 185 611 82 504 3.20e-68 232
MS.gene064122.t1 AT1G14970 33.103 435 255 9 198 610 12 432 3.29e-68 229
MS.gene064122.t1 AT2G03280 35.500 400 215 8 231 592 92 486 2.56e-67 228
MS.gene064122.t1 AT1G14970 33.103 435 255 9 198 610 132 552 1.66e-66 228
MS.gene064122.t1 AT3G02250 35.092 379 220 6 230 592 109 477 1.78e-66 226
MS.gene064122.t1 AT1G14970 33.495 412 246 7 220 610 2 406 3.89e-66 222
MS.gene064122.t1 AT3G26370 33.920 398 243 8 230 610 161 555 1.03e-65 225
MS.gene064122.t1 AT1G04910 31.308 428 267 11 196 610 61 474 1.16e-64 221
MS.gene064122.t1 AT1G35510 32.346 439 261 9 196 612 134 558 1.33e-63 220
MS.gene064122.t1 AT1G38065 33.092 414 253 10 218 610 18 428 1.69e-63 217
MS.gene064122.t1 AT1G38065 33.333 402 245 9 230 610 39 438 2.33e-63 216
MS.gene064122.t1 AT1G38131 33.092 414 253 10 218 610 147 557 2.02e-62 217
MS.gene064122.t1 AT2G01480 32.614 417 253 8 214 609 141 550 1.42e-61 214
MS.gene064122.t1 AT2G01480 33.600 375 226 5 214 569 141 511 1.00e-59 208
MS.gene064122.t1 AT1G62330 29.046 482 259 15 193 612 191 651 6.09e-54 195
MS.gene064122.t1 AT1G11990 31.377 443 267 15 193 611 160 589 4.48e-52 189
MS.gene064122.t1 AT1G29200 32.829 463 234 18 206 610 50 493 6.46e-48 175
MS.gene064122.t1 AT1G29200 32.829 463 234 18 206 610 50 493 6.46e-48 175
MS.gene064122.t1 AT1G29200 32.829 463 234 18 206 610 166 609 2.22e-47 176
MS.gene064122.t1 AT1G11990 28.958 480 267 15 193 611 145 611 5.51e-47 175
MS.gene064122.t1 AT1G29200 33.565 432 221 16 232 610 1 419 1.67e-46 169
MS.gene064122.t1 AT3G30300 32.414 290 181 6 230 504 103 392 1.71e-34 139
MS.gene064122.t1 AT3G30300 32.414 290 181 6 230 504 103 392 1.71e-34 139
MS.gene064122.t1 AT1G52630 24.550 444 285 14 178 610 31 435 3.72e-30 124
MS.gene064122.t1 AT3G26370 33.516 182 107 4 230 398 161 341 2.28e-29 120
MS.gene064122.t1 AT3G03810 30.034 293 184 7 230 504 92 381 1.72e-26 115
MS.gene064122.t1 AT3G03810 30.034 293 184 7 230 504 92 381 1.72e-26 115
MS.gene064122.t1 AT1G52630 25.066 379 240 12 243 610 2 347 7.70e-26 109

Find 141 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTGGTTGTTTC+TGG -0.000901 4.3:-75503419 MS.gene064122:CDS
ACCAAACCCATTTGAGATTA+AGG 0.186427 4.3:+75503808 None:intergenic
GCCATCTCATGGAGGAAATA+TGG 0.218103 4.3:-75501527 MS.gene064122:CDS
GTTGTTATTGCTAGGATTCT+TGG 0.223923 4.3:-75503359 MS.gene064122:CDS
GAGAGAAGAATTCAGAAAAT+AGG 0.237193 4.3:+75500966 None:intergenic
ATGACATTAACTTGCAAAAT+AGG 0.247264 4.3:+75503320 None:intergenic
TCTTCACCACCTTAACAAAA+TGG 0.248210 4.3:-75503958 MS.gene064122:CDS
TATAGGTTGCTTAAAGCTTT+AGG 0.261962 4.3:-75501754 MS.gene064122:intron
AGAGAAGCTTTCTTCGAAAA+TGG 0.262334 4.3:+75501598 None:intergenic
GACCAGCCATCTTTCTTTCA+TGG 0.284935 4.3:+75502184 None:intergenic
GTTTCCACATCTTTATAATA+AGG 0.294769 4.3:-75501650 MS.gene064122:CDS
AAAAGATGCAAGAATTTATT+GGG 0.322642 4.3:-75501725 MS.gene064122:CDS
AAATATGGGCCATGCGCTTC+AGG 0.329180 4.3:-75501512 MS.gene064122:intron
GAAAAGCCCTTAATCTCAAA+TGG 0.333986 4.3:-75503815 MS.gene064122:CDS
AGAGAAGAATTCAGAAAATA+GGG 0.334594 4.3:+75500967 None:intergenic
CCAAACCCATTTGAGATTAA+GGG 0.341142 4.3:+75503809 None:intergenic
CAAAACCCTTCAATCTCAAA+TGG 0.341182 4.3:-75503698 MS.gene064122:CDS
TCAAATTCACTTCCTTTGAT+TGG 0.342422 4.3:-75503605 MS.gene064122:CDS
CCCTTAATCTCAAATGGGTT+TGG 0.349127 4.3:-75503809 MS.gene064122:CDS
CTCAGAAACTATGTAATCTA+TGG 0.350072 4.3:+75501567 None:intergenic
TGGAAGCAACCAGATGGGTT+AGG 0.362833 4.3:-75503515 MS.gene064122:CDS
TAGGATTCTTGGTGCTGCTT+TGG 0.371467 4.3:-75503348 MS.gene064122:CDS
ATGCTGTTAAACTCTGCTTC+AGG 0.374694 4.3:+75500943 None:intergenic
TTGAATAGAAGAAGATGAAA+TGG 0.388256 4.3:+75504039 None:intergenic
TGGTTCAAAATCTGAACTTA+AGG 0.388422 4.3:-75503789 MS.gene064122:CDS
TTCGAAGAAAGCTTCTCTAA+TGG 0.388722 4.3:-75501593 MS.gene064122:CDS
ATGAAGAAAAGGGTGAGTTT+TGG 0.390118 4.3:-75503535 MS.gene064122:CDS
GTTAGGGTATAAGCCTTGTT+TGG 0.390330 4.3:-75503498 MS.gene064122:CDS
GTGAGGACTGGTTGTCTACC+TGG 0.392388 4.3:-75502291 MS.gene064122:CDS
GAATTGCATCAGAATTCATT+AGG 0.395674 4.3:-75500913 MS.gene064122:CDS
TGTGGAAACTCATGGATTAA+TGG 0.400222 4.3:+75501664 None:intergenic
TTGTTTCCAAGTTCTTGAAC+TGG 0.412948 4.3:+75502384 None:intergenic
ACCTTGCTAGTCCTGAGTTC+CGG 0.418631 4.3:+75500886 None:intergenic
GCGAAACCAGTTCAAGAACT+TGG 0.420208 4.3:-75502390 MS.gene064122:CDS
TCCGTTCGCGCTATCTCAAA+AGG 0.424692 4.3:-75502857 MS.gene064122:CDS
GAATAGAAAGAGGTATCTTA+TGG 0.428527 4.3:-75503432 MS.gene064122:CDS
CGAAACCACCCGCACATCAT+TGG 0.433723 4.3:+75502953 None:intergenic
GGAAGCAACCAGATGGGTTA+GGG 0.436626 4.3:-75503514 MS.gene064122:CDS
TTTGTCATCAAGTGTGTTGA+TGG 0.436659 4.3:+75502924 None:intergenic
TCAAACCCATTTGAGATTGA+AGG 0.439951 4.3:+75503692 None:intergenic
GGTGGTGAAGAAGAAGTTAG+AGG 0.440438 4.3:+75503970 None:intergenic
ATGCAAGAATTTATTGGGCC+GGG 0.443277 4.3:-75501720 MS.gene064122:CDS
GTATTTCTTGTGTCCTGCAT+AGG 0.451809 4.3:+75501017 None:intergenic
TGACTTGGCGGATGAAGAAA+AGG 0.456333 4.3:-75503546 MS.gene064122:CDS
GGCTTATACCCTAACCCATC+TGG 0.457469 4.3:+75503506 None:intergenic
GTTATTCCTTCCAGGGTCAC+AGG 0.458415 4.3:-75501041 MS.gene064122:intron
TATAAAGATGTGGAAACTCA+TGG 0.469538 4.3:+75501656 None:intergenic
AAGGAAGATCTTGCATTGCC+TGG 0.471250 4.3:-75501631 MS.gene064122:CDS
TCCACCCAATGGTTGTCCCC+CGG 0.472197 4.3:+75501702 None:intergenic
AAAACCCTTCAATCTCAAAT+GGG 0.474207 4.3:-75503697 MS.gene064122:CDS
TGCTGTTAAACTCTGCTTCA+GGG 0.476049 4.3:+75500944 None:intergenic
CCATCTCATGGAGGAAATAT+GGG 0.480168 4.3:-75501526 MS.gene064122:CDS
TAGCGCGAACGGATCCAACT+TGG 0.483932 4.3:+75502867 None:intergenic
AAAAGCCCTTAATCTCAAAT+GGG 0.485351 4.3:-75503814 MS.gene064122:CDS
CCTTGCTCTTCATCTAAGAA+TGG 0.490124 4.3:-75502328 MS.gene064122:CDS
CTTATGGTGGTTGTTTCTGG+AGG 0.490684 4.3:-75503416 MS.gene064122:CDS
GATGCAAGAATTTATTGGGC+CGG 0.492621 4.3:-75501721 MS.gene064122:CDS
AAGGATGTGTGGGTGAGGAC+TGG 0.493609 4.3:-75502303 MS.gene064122:CDS
CAAACCCATTTGAGATTGAA+GGG 0.494557 4.3:+75503693 None:intergenic
GATCTTCCTTTCAAGAGCTC+CGG 0.494862 4.3:+75502219 None:intergenic
CTCTTCATGTCACTCCAAGT+TGG 0.504620 4.3:-75502881 MS.gene064122:CDS
TGCAAGAATTTATTGGGCCG+GGG 0.505342 4.3:-75501719 MS.gene064122:CDS
GAAAATGGTTCTAGTTCTCC+AGG 0.505643 4.3:+75501613 None:intergenic
AACTCAGGACTAGCAAGGTC+GGG 0.506943 4.3:-75500882 MS.gene064122:CDS
GGTTCCTAGTGGAGTTGACT+TGG 0.507131 4.3:-75503561 MS.gene064122:CDS
AGCCTTGTTTGGATTTCAGT+AGG 0.508226 4.3:-75503487 MS.gene064122:CDS
TTGCACATGCACTCTGGGAC+AGG 0.510427 4.3:+75500841 None:intergenic
CCTTTGCAGTTCAACAGAGA+AGG 0.513180 4.3:-75502597 MS.gene064122:intron
TCTTTGTCCCTTGACTGCCA+TGG 0.516067 4.3:-75502163 MS.gene064122:CDS
TTTGCAAGTTAATGTCATCT+GGG 0.518250 4.3:-75503315 MS.gene064122:CDS
TCTTCATCTAAGAATGGAAA+AGG 0.529212 4.3:-75502322 MS.gene064122:CDS
GCCTTGTTTGGATTTCAGTA+GGG 0.532530 4.3:-75503486 MS.gene064122:CDS
TCCTAGTGGAGTTGACTTGG+CGG 0.533948 4.3:-75503558 MS.gene064122:CDS
GGTAACATCCATGGCAGTCA+AGG 0.535475 4.3:+75502155 None:intergenic
TCTTAGATGAAGAGCAAGGT+AGG 0.537764 4.3:+75502332 None:intergenic
AACATTACATTACCAATCAA+AGG 0.538428 4.3:+75503593 None:intergenic
GCTATCGAAGGATCTTCCTT+CGG 0.538608 4.3:-75502544 MS.gene064122:CDS
GAATGAGAGGAGAGAACGAC+CGG 0.540471 4.3:-75502238 MS.gene064122:CDS
TATTTACCTGGTAACATCCA+TGG 0.544732 4.3:+75502146 None:intergenic
CAGGGTCACAGGGCCTATGC+AGG 0.546366 4.3:-75501030 MS.gene064122:CDS
TCAGAATTCATTAGGACAGC+CGG 0.547453 4.3:-75500905 MS.gene064122:CDS
TGGAAAAGGATGTGTGGGTG+AGG 0.548660 4.3:-75502308 MS.gene064122:CDS
TAATGTAATGTTGCAAGCAA+AGG 0.548895 4.3:-75503582 MS.gene064122:CDS
GAGTCGTTGCACATGCACTC+TGG 0.553547 4.3:+75500835 None:intergenic
CATTAGGACAGCCGGAACTC+AGG 0.555136 4.3:-75500897 MS.gene064122:CDS
AGAGGAGGACTTCCCTCCAC+TGG 0.555343 4.3:+75502900 None:intergenic
TTGATGCTGTTGTTATTGCT+AGG 0.555554 4.3:-75503367 MS.gene064122:CDS
ACGAGATTGTAAAGAATGAG+AGG 0.556539 4.3:-75502251 MS.gene064122:CDS
GAACGACCGGAGCTCTTGAA+AGG 0.557470 4.3:-75502225 MS.gene064122:CDS
ATTACAGAAGAGAAAGTGAT+GGG 0.558110 4.3:-75503463 MS.gene064122:CDS
TTTACAATCTCGTCAAACTC+AGG 0.561693 4.3:+75502261 None:intergenic
GATTACAGAAGAGAAAGTGA+TGG 0.561987 4.3:-75503464 MS.gene064122:CDS
TACCATGAAAGAAAGATGGC+TGG 0.562902 4.3:-75502186 MS.gene064122:CDS
CCCATATTTCCTCCATGAGA+TGG 0.566943 4.3:+75501526 None:intergenic
ATGGAATTGATTATTTGTGA+AGG 0.568945 4.3:+75504058 None:intergenic
TTATGGTGGTTGTTTCTGGA+GGG 0.569275 4.3:-75503415 MS.gene064122:CDS
TTTGATTCAAAATCTGAACT+TGG 0.571345 4.3:-75503674 MS.gene064122:CDS
CAACTTGGAGTGACATGAAG+AGG 0.572279 4.3:+75502882 None:intergenic
TTCAACATTTGTAGCACATG+GGG 0.574252 4.3:+75503835 None:intergenic
ATGTCATCTGGGGAGATGAA+AGG 0.574871 4.3:-75503304 MS.gene064122:intron
AGTCGTTGCACATGCACTCT+GGG 0.575723 4.3:+75500836 None:intergenic
AAAAGAAGAACAATCTTGAA+GGG 0.576810 4.3:+75503930 None:intergenic
CCGTTCGCGCTATCTCAAAA+GGG 0.577744 4.3:-75502856 MS.gene064122:intron
GACTTACCATGAAAGAAAGA+TGG 0.579868 4.3:-75502190 MS.gene064122:CDS
TCTTCAGAAGCTTCGATGCA+AGG 0.581403 4.3:-75502520 MS.gene064122:intron
GAACTCAGGACTAGCAAGGT+CGG 0.582708 4.3:-75500883 MS.gene064122:CDS
TTATTCCTTCCAGGGTCACA+GGG 0.583835 4.3:-75501040 MS.gene064122:intron
TGTTCTTGCCAATGATGTGC+GGG 0.584306 4.3:-75502961 MS.gene064122:CDS
TCTTCCTTATTATAAAGATG+TGG 0.591443 4.3:+75501646 None:intergenic
TGACTGCCATGGATGTTACC+AGG 0.594053 4.3:-75502152 MS.gene064122:intron
TTTCAACATTTGTAGCACAT+GGG 0.594469 4.3:+75503834 None:intergenic
GCAAGAATTTATTGGGCCGG+GGG 0.602690 4.3:-75501718 MS.gene064122:CDS
CGAAGCTTCTGAAGATCCGA+AGG 0.604591 4.3:+75502528 None:intergenic
TCAGGACTAGCAAGGTCGGG+AGG 0.608590 4.3:-75500879 MS.gene064122:CDS
TTGCAAGTTAATGTCATCTG+GGG 0.612484 4.3:-75503314 MS.gene064122:CDS
CTTAGATGAAGAGCAAGGTA+GGG 0.612955 4.3:+75502333 None:intergenic
TAGAAAGAGGTATCTTATGG+TGG 0.614327 4.3:-75503429 MS.gene064122:CDS
CCATTCTTAGATGAAGAGCA+AGG 0.618532 4.3:+75502328 None:intergenic
CTTGGATTCAAGGCTATCGA+AGG 0.620323 4.3:-75502556 MS.gene064122:CDS
GTAACATCCATGGCAGTCAA+GGG 0.621567 4.3:+75502156 None:intergenic
TGTTTCAATGAATAAAGATG+AGG 0.622634 4.3:-75503648 MS.gene064122:CDS
CTTGATGACAAAGCCAGTGG+AGG 0.623445 4.3:-75502913 MS.gene064122:CDS
GCTGAGAGAATGCAAAGCAA+AGG 0.624230 4.3:-75502357 MS.gene064122:CDS
TTGATGACAAAGCCAGTGGA+GGG 0.626586 4.3:-75502912 MS.gene064122:CDS
AAGAATGGAAAAGGATGTGT+GGG 0.627477 4.3:-75502313 MS.gene064122:CDS
TCAAACTCAGGACTCAGACC+AGG 0.627651 4.3:+75502273 None:intergenic
CAGGACTAGCAAGGTCGGGA+GGG 0.628531 4.3:-75500878 MS.gene064122:CDS
TCCGCCAAGTCAACTCCACT+AGG 0.635742 4.3:+75503557 None:intergenic
TTTGGTAAAATCTGAACTTG+GGG 0.638044 4.3:-75503726 MS.gene064122:CDS
GACTTGGCGGATGAAGAAAA+GGG 0.646979 4.3:-75503545 MS.gene064122:CDS
CTTGGAGTGACATGAAGAGG+AGG 0.650220 4.3:+75502885 None:intergenic
TTGCAAGCAAAGGTTCCTAG+TGG 0.655758 4.3:-75503572 MS.gene064122:CDS
TCCCTACTGAAATCCAAACA+AGG 0.655775 4.3:+75503485 None:intergenic
TAAGAATGGAAAAGGATGTG+TGG 0.664578 4.3:-75502314 MS.gene064122:CDS
GCCGGAACTCAGGACTAGCA+AGG 0.665088 4.3:-75500887 MS.gene064122:CDS
GGATTGTTAAGAATAGAAAG+AGG 0.665202 4.3:-75503442 MS.gene064122:CDS
CTGGAGGGATGAATCAGCAG+AGG 0.666148 4.3:-75503400 MS.gene064122:CDS
ATAGTCATACCTGAAGCGCA+TGG 0.668438 4.3:+75501503 None:intergenic
ATGTTCTTGCCAATGATGTG+CGG 0.671242 4.3:-75502962 MS.gene064122:CDS
ACACTTGATGACAAAGCCAG+TGG 0.673399 4.3:-75502916 MS.gene064122:CDS
TCTTGCCAATGATGTGCGGG+TGG 0.696204 4.3:-75502958 MS.gene064122:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGAGATTTTTAA+TGG - chr4.3:75501170-75501189 MS.gene064122:intron 10.0%
!! TTATTAAATATGAAGACTAA+AGG + chr4.3:75503630-75503649 None:intergenic 15.0%
!!! ATGATACAATCAATTATTTT+AGG - chr4.3:75503683-75503702 MS.gene064122:CDS 15.0%
!!! TGATACAATCAATTATTTTA+GGG - chr4.3:75503684-75503703 MS.gene064122:CDS 15.0%
!!! TTTATTTGTTTTGGATTTAT+AGG - chr4.3:75503154-75503173 MS.gene064122:intron 15.0%
!! AAAAAGATGCAAGAATTTAT+TGG - chr4.3:75503199-75503218 MS.gene064122:intron 20.0%
!! AAAAGATGCAAGAATTTATT+GGG - chr4.3:75503200-75503219 MS.gene064122:intron 20.0%
!! AAACAAAACCAAAATCAATA+AGG + chr4.3:75501900-75501919 None:intergenic 20.0%
!! AATATATGAAGATTTATGCA+AGG - chr4.3:75501699-75501718 MS.gene064122:CDS 20.0%
!! AATTGCATGATTACAATTTA+TGG + chr4.3:75503754-75503773 None:intergenic 20.0%
!! AGAAGAATTTAATATTTACC+TGG + chr4.3:75502794-75502813 None:intergenic 20.0%
!! TTACACTTATGAAACTATTT+GGG - chr4.3:75502867-75502886 MS.gene064122:CDS 20.0%
!! TTCTGTGGAAATAAAAATTT+GGG - chr4.3:75502261-75502280 MS.gene064122:CDS 20.0%
!!! AAAAATGAGATTTTTAATGG+TGG - chr4.3:75501173-75501192 MS.gene064122:intron 20.0%
!!! AAATTCTTCTACTTTTGTAT+AGG - chr4.3:75502804-75502823 MS.gene064122:intron 20.0%
!!! AGAGTTTTGTTTATTTGTTT+TGG - chr4.3:75503145-75503164 MS.gene064122:intron 20.0%
!!! ATGTTTTTCCTTATTGATTT+TGG - chr4.3:75501889-75501908 MS.gene064122:intron 20.0%
!!! ATTTTTGTTGAAGATTTCTT+TGG + chr4.3:75500933-75500952 None:intergenic 20.0%
!!! TGTGCTTTTAGTAATTAAAA+AGG - chr4.3:75503656-75503675 MS.gene064122:CDS 20.0%
!!! TTATGGAGAAAATATGTTTT+TGG + chr4.3:75503737-75503756 None:intergenic 20.0%
! AAAAAGAAGAACAATCTTGA+AGG + chr4.3:75500999-75501018 None:intergenic 25.0%
! AAAAGAAGAACAATCTTGAA+GGG + chr4.3:75500998-75501017 None:intergenic 25.0%
! AACATTACATTACCAATCAA+AGG + chr4.3:75501335-75501354 None:intergenic 25.0%
! AATTAAGATAGTCACATTGA+TGG - chr4.3:75503770-75503789 MS.gene064122:CDS 25.0%
! AGAGAAGAATTCAGAAAATA+GGG + chr4.3:75503961-75503980 None:intergenic 25.0%
! ATACAGTATATATGAAAGCA+TGG + chr4.3:75503542-75503561 None:intergenic 25.0%
! ATCTTCATTTGTGTAACAAT+CGG - chr4.3:75502448-75502467 MS.gene064122:intron 25.0%
! ATGGAATTGATTATTTGTGA+AGG + chr4.3:75500870-75500889 None:intergenic 25.0%
! CTTACACTTATGAAACTATT+TGG - chr4.3:75502866-75502885 MS.gene064122:CDS 25.0%
! CTTAGATTATGTTCCAATAT+TGG + chr4.3:75501764-75501783 None:intergenic 25.0%
! CTTCTGTGGAAATAAAAATT+TGG - chr4.3:75502260-75502279 MS.gene064122:CDS 25.0%
! GTAATCATGCTTACATATAT+TGG - chr4.3:75503516-75503535 MS.gene064122:CDS 25.0%
! GTTTCCACATCTTTATAATA+AGG - chr4.3:75503275-75503294 MS.gene064122:intron 25.0%
! TACTATACTATTCTTGTTCA+TGG - chr4.3:75501651-75501670 MS.gene064122:CDS 25.0%
! TCTTCCTTATTATAAAGATG+TGG + chr4.3:75503282-75503301 None:intergenic 25.0%
! TGTTTCAATGAATAAAGATG+AGG - chr4.3:75501277-75501296 MS.gene064122:intron 25.0%
! TTGAATAGAAGAAGATGAAA+TGG + chr4.3:75500889-75500908 None:intergenic 25.0%
! TTTGATTCAAAATCTGAACT+TGG - chr4.3:75501251-75501270 MS.gene064122:intron 25.0%
!! AAGGTGAGTACAAATTTTAT+AGG + chr4.3:75503611-75503630 None:intergenic 25.0%
!! AGGTGAGTACAAATTTTATA+GGG + chr4.3:75503610-75503629 None:intergenic 25.0%
!! CTCTTATTTGAGTTTATTAC+TGG - chr4.3:75502841-75502860 MS.gene064122:intron 25.0%
!! TTTTGGTAAAATCTGAACTT+GGG - chr4.3:75501198-75501217 MS.gene064122:intron 25.0%
!!! AAAGATGAGGTTTTGAATAA+AGG - chr4.3:75501290-75501309 MS.gene064122:intron 25.0%
!!! AATTATTTTAGGGATGTTGA+TGG - chr4.3:75503694-75503713 MS.gene064122:CDS 25.0%
!!! ATGACATTAACTTGCAAAAT+AGG + chr4.3:75501608-75501627 None:intergenic 25.0%
!!! ATTATTTTAGGGATGTTGAT+GGG - chr4.3:75503695-75503714 MS.gene064122:CDS 25.0%
!!! GTTTTTGCTTTGTTTATGAT+TGG - chr4.3:75501038-75501057 MS.gene064122:intron 25.0%
!!! TTTTTCACACTCTTATTTCT+TGG - chr4.3:75501014-75501033 MS.gene064122:CDS 25.0%
AAAACCCTTCAATCTCAAAT+GGG - chr4.3:75501228-75501247 MS.gene064122:intron 30.0%
AAAAGCCCTTAATCTCAAAT+GGG - chr4.3:75501111-75501130 MS.gene064122:intron 30.0%
AATAAGCTGGAAACAGTTTA+TGG + chr4.3:75502922-75502941 None:intergenic 30.0%
ATCAAGGAAAAACTATGCTT+AGG + chr4.3:75501787-75501806 None:intergenic 30.0%
ATTACAGAAGAGAAAGTGAT+GGG - chr4.3:75501462-75501481 MS.gene064122:intron 30.0%
CAAAAACTTAGCAACAACTT+AGG + chr4.3:75502144-75502163 None:intergenic 30.0%
CTCAGAAACTATGTAATCTA+TGG + chr4.3:75503361-75503380 None:intergenic 30.0%
GAATTGCATCAGAATTCATT+AGG - chr4.3:75504012-75504031 MS.gene064122:CDS 30.0%
GAGAGAAGAATTCAGAAAAT+AGG + chr4.3:75503962-75503981 None:intergenic 30.0%
GGATTGTTAAGAATAGAAAG+AGG - chr4.3:75501483-75501502 MS.gene064122:intron 30.0%
TAAAAGCAACCTGAAAACAT+AGG + chr4.3:75502501-75502520 None:intergenic 30.0%
TAACACACTTTCTCATCAAA+GGG + chr4.3:75502204-75502223 None:intergenic 30.0%
TAATGTAATGTTGCAAGCAA+AGG - chr4.3:75501343-75501362 MS.gene064122:intron 30.0%
TATAAAGATGTGGAAACTCA+TGG + chr4.3:75503272-75503291 None:intergenic 30.0%
TATAGGTTGCTTAAAGCTTT+AGG - chr4.3:75503171-75503190 MS.gene064122:intron 30.0%
TCAAATTCACTTCCTTTGAT+TGG - chr4.3:75501320-75501339 MS.gene064122:intron 30.0%
TCTTCATCTAAGAATGGAAA+AGG - chr4.3:75502603-75502622 MS.gene064122:intron 30.0%
TCTTCTAGCATTCACAAATA+AGG + chr4.3:75501850-75501869 None:intergenic 30.0%
TGGTTCAAAATCTGAACTTA+AGG - chr4.3:75501136-75501155 MS.gene064122:intron 30.0%
TGTTTATCCAAAGATCCTAT+AGG - chr4.3:75503085-75503104 MS.gene064122:intron 30.0%
TTAACACACTTTCTCATCAA+AGG + chr4.3:75502205-75502224 None:intergenic 30.0%
TTATAGGGACTAAAGTTGAA+AGG + chr4.3:75503595-75503614 None:intergenic 30.0%
TTTCAACATTTGTAGCACAT+GGG + chr4.3:75501094-75501113 None:intergenic 30.0%
TTTGCAAGTTAATGTCATCT+GGG - chr4.3:75501610-75501629 MS.gene064122:CDS 30.0%
TTTGGACTTAAATCTTCCTA+AGG - chr4.3:75501737-75501756 MS.gene064122:CDS 30.0%
TTTGGTAAAATCTGAACTTG+GGG - chr4.3:75501199-75501218 MS.gene064122:intron 30.0%
! ACTGATTTCATAAGCTATCT+TGG + chr4.3:75502957-75502976 None:intergenic 30.0%
! AGGGTTTTTCCATTTTGTTA+AGG + chr4.3:75500979-75500998 None:intergenic 30.0%
! AGGTTCAGTTGTTACAATTT+TGG - chr4.3:75501719-75501738 MS.gene064122:CDS 30.0%
! GTTTTTCCATTTTGTTAAGG+TGG + chr4.3:75500976-75500995 None:intergenic 30.0%
! TTTTCAACATTTGTAGCACA+TGG + chr4.3:75501095-75501114 None:intergenic 30.0%
! TTTTGCAAGTTAATGTCATC+TGG - chr4.3:75501609-75501628 MS.gene064122:CDS 30.0%
!! ACTCATGTTTTTGCATCTTT+AGG - chr4.3:75502111-75502130 MS.gene064122:intron 30.0%
!! ATGCATTTGTCTTTTGTTTG+GGG + chr4.3:75503938-75503957 None:intergenic 30.0%
!! GAATAGAAAGAGGTATCTTA+TGG - chr4.3:75501493-75501512 MS.gene064122:intron 30.0%
!! GATGCATTTGTCTTTTGTTT+GGG + chr4.3:75503939-75503958 None:intergenic 30.0%
!! GATTTTTAATGGTGGTGTTT+TGG - chr4.3:75501181-75501200 MS.gene064122:intron 30.0%
!! GTTTTGGTAAAATCTGAACT+TGG - chr4.3:75501197-75501216 MS.gene064122:intron 30.0%
AAACTATTTGGGAGAGCTTA+TGG - chr4.3:75502878-75502897 MS.gene064122:CDS 35.0%
AAGAATGGAAAAGGATGTGT+GGG - chr4.3:75502612-75502631 MS.gene064122:intron 35.0%
ACCTAAATGTTATTCCTTCC+AGG - chr4.3:75503876-75503895 MS.gene064122:CDS 35.0%
ACGAAACATAACCAGTATCA+AGG + chr4.3:75501803-75501822 None:intergenic 35.0%
ACGAGATTGTAAAGAATGAG+AGG - chr4.3:75502674-75502693 MS.gene064122:intron 35.0%
AGAGAAGCTTTCTTCGAAAA+TGG + chr4.3:75503330-75503349 None:intergenic 35.0%
ATCTTCCTAAGGACCAATAT+TGG - chr4.3:75501748-75501767 MS.gene064122:CDS 35.0%
ATGTTCCAATATTGGTCCTT+AGG + chr4.3:75501756-75501775 None:intergenic 35.0%
CAAAACCCTTCAATCTCAAA+TGG - chr4.3:75501227-75501246 MS.gene064122:intron 35.0%
CCTAAATGTTATTCCTTCCA+GGG - chr4.3:75503877-75503896 MS.gene064122:CDS 35.0%
GAAAAGCCCTTAATCTCAAA+TGG - chr4.3:75501110-75501129 MS.gene064122:intron 35.0%
GACTTACCATGAAAGAAAGA+TGG - chr4.3:75502735-75502754 MS.gene064122:intron 35.0%
GATTACAGAAGAGAAAGTGA+TGG - chr4.3:75501461-75501480 MS.gene064122:intron 35.0%
TAAGAATGGAAAAGGATGTG+TGG - chr4.3:75502611-75502630 MS.gene064122:intron 35.0%
TATTTACCTGGTAACATCCA+TGG + chr4.3:75502782-75502801 None:intergenic 35.0%
TCTTCACCACCTTAACAAAA+TGG - chr4.3:75500967-75500986 MS.gene064122:CDS 35.0%
TGTTGAGCTTGAGTTTAACA+TGG - chr4.3:75502295-75502314 MS.gene064122:CDS 35.0%
TTCAACATTTGTAGCACATG+GGG + chr4.3:75501093-75501112 None:intergenic 35.0%
TTCGAAGAAAGCTTCTCTAA+TGG - chr4.3:75503332-75503351 MS.gene064122:CDS 35.0%
TTGCAAGTTAATGTCATCTG+GGG - chr4.3:75501611-75501630 MS.gene064122:CDS 35.0%
TTGTTTCCAAGTTCTTGAAC+TGG + chr4.3:75502544-75502563 None:intergenic 35.0%
TTTACAATCTCGTCAAACTC+AGG + chr4.3:75502667-75502686 None:intergenic 35.0%
TTTGTCATCAAGTGTGTTGA+TGG + chr4.3:75502004-75502023 None:intergenic 35.0%
! AATGAAAGCTTTGACTTCTG+TGG - chr4.3:75502246-75502265 MS.gene064122:CDS 35.0%
! ACCAAACCCATTTGAGATTA+AGG + chr4.3:75501120-75501139 None:intergenic 35.0%
! ATGAAGAAAAGGGTGAGTTT+TGG - chr4.3:75501390-75501409 MS.gene064122:intron 35.0%
! CAAACCCATTTGAGATTGAA+GGG + chr4.3:75501235-75501254 None:intergenic 35.0%
! CCAAACCCATTTGAGATTAA+GGG + chr4.3:75501119-75501138 None:intergenic 35.0%
! CTTTTGTATAGGAGTCTGTT+TGG - chr4.3:75502815-75502834 MS.gene064122:intron 35.0%
! GCATAGTTTTTCCTTGATAC+TGG - chr4.3:75501789-75501808 MS.gene064122:intron 35.0%
! GGATGCATTTGTCTTTTGTT+TGG + chr4.3:75503940-75503959 None:intergenic 35.0%
! GTTCATGGTTTGTGATATTG+TGG - chr4.3:75501666-75501685 MS.gene064122:CDS 35.0%
! GTTGTTATTGCTAGGATTCT+TGG - chr4.3:75501566-75501585 MS.gene064122:CDS 35.0%
! TATCTTATGGTGGTTGTTTC+TGG - chr4.3:75501506-75501525 MS.gene064122:intron 35.0%
! TCAAACCCATTTGAGATTGA+AGG + chr4.3:75501236-75501255 None:intergenic 35.0%
! TGTGGAAACTCATGGATTAA+TGG + chr4.3:75503264-75503283 None:intergenic 35.0%
! TTGATGCTGTTGTTATTGCT+AGG - chr4.3:75501558-75501577 MS.gene064122:CDS 35.0%
!! TAGAAAGAGGTATCTTATGG+TGG - chr4.3:75501496-75501515 MS.gene064122:intron 35.0%
!! TTGATGGGTCGTGATTTTAT+AGG - chr4.3:75503710-75503729 MS.gene064122:CDS 35.0%
!!! GCACATGGGGTTTTAAAAAA+CGG + chr4.3:75501080-75501099 None:intergenic 35.0%
AGTGAAGCCTATAGGATCTT+TGG + chr4.3:75503095-75503114 None:intergenic 40.0%
ATGCAAGAATTTATTGGGCC+GGG - chr4.3:75503205-75503224 MS.gene064122:intron 40.0%
ATGCTGTTAAACTCTGCTTC+AGG + chr4.3:75503985-75504004 None:intergenic 40.0%
ATGTTCTTGCCAATGATGTG+CGG - chr4.3:75501963-75501982 MS.gene064122:intron 40.0%
CACTGAAAAGTGAAGCCTAT+AGG + chr4.3:75503103-75503122 None:intergenic 40.0%
CCATCTCATGGAGGAAATAT+GGG - chr4.3:75503399-75503418 MS.gene064122:CDS 40.0%
CCATTCTTAGATGAAGAGCA+AGG + chr4.3:75502600-75502619 None:intergenic 40.0%
CCCTGGAAGGAATAACATTT+AGG + chr4.3:75503880-75503899 None:intergenic 40.0%
CCTTGCTCTTCATCTAAGAA+TGG - chr4.3:75502597-75502616 MS.gene064122:intron 40.0%
CTTAGATGAAGAGCAAGGTA+GGG + chr4.3:75502595-75502614 None:intergenic 40.0%
GATGCAAGAATTTATTGGGC+CGG - chr4.3:75503204-75503223 MS.gene064122:intron 40.0%
GTATTTCTTGTGTCCTGCAT+AGG + chr4.3:75503911-75503930 None:intergenic 40.0%
TACCATGAAAGAAAGATGGC+TGG - chr4.3:75502739-75502758 MS.gene064122:intron 40.0%
TCAGAATTCATTAGGACAGC+CGG - chr4.3:75504020-75504039 MS.gene064122:CDS 40.0%
TCCCTACTGAAATCCAAACA+AGG + chr4.3:75501443-75501462 None:intergenic 40.0%
TCTTAGATGAAGAGCAAGGT+AGG + chr4.3:75502596-75502615 None:intergenic 40.0%
TGCTGTTAAACTCTGCTTCA+GGG + chr4.3:75503984-75504003 None:intergenic 40.0%
! AAAGCTTCTTTTCCACCCAA+TGG + chr4.3:75503237-75503256 None:intergenic 40.0%
! CCCTTAATCTCAAATGGGTT+TGG - chr4.3:75501116-75501135 MS.gene064122:intron 40.0%
! CTCATCTCACCTATGTTTTC+AGG - chr4.3:75502489-75502508 MS.gene064122:intron 40.0%
! GATGTTTTCATGCCATCTCA+TGG - chr4.3:75503387-75503406 MS.gene064122:CDS 40.0%
! TTTTACGCAGCTTGGATTCA+AGG - chr4.3:75502359-75502378 MS.gene064122:CDS 40.0%
!! AGCCTTGTTTGGATTTCAGT+AGG - chr4.3:75501438-75501457 MS.gene064122:intron 40.0%
!! CTATAGGCTTCACTTTTCAG+TGG - chr4.3:75503101-75503120 MS.gene064122:intron 40.0%
!! GAAAATGGTTCTAGTTCTCC+AGG + chr4.3:75503315-75503334 None:intergenic 40.0%
!! GCCTTGTTTGGATTTCAGTA+GGG - chr4.3:75501439-75501458 MS.gene064122:intron 40.0%
!! GTTAGGGTATAAGCCTTGTT+TGG - chr4.3:75501427-75501446 MS.gene064122:intron 40.0%
!! TGTTTTGCTTTTACGCAGCT+TGG - chr4.3:75502351-75502370 MS.gene064122:CDS 40.0%
!! TTATGGTGGTTGTTTCTGGA+GGG - chr4.3:75501510-75501529 MS.gene064122:intron 40.0%
!!! AGTTTTGGAAGCAACCAGAT+GGG - chr4.3:75501405-75501424 MS.gene064122:intron 40.0%
AAGCTATCTTGGAGAGCTTG+TGG + chr4.3:75502946-75502965 None:intergenic 45.0%
AAGGAAGATCTTGCATTGCC+TGG - chr4.3:75503294-75503313 MS.gene064122:intron 45.0%
AGCTATCTTGGAGAGCTTGT+GGG + chr4.3:75502945-75502964 None:intergenic 45.0%
ATAGTCATACCTGAAGCGCA+TGG + chr4.3:75503425-75503444 None:intergenic 45.0%
ATGTCATCTGGGGAGATGAA+AGG - chr4.3:75501621-75501640 MS.gene064122:CDS 45.0%
CAACTTGGAGTGACATGAAG+AGG + chr4.3:75502046-75502065 None:intergenic 45.0%
CCCATATTTCCTCCATGAGA+TGG + chr4.3:75503402-75503421 None:intergenic 45.0%
CCTTCTCTGTTGAACTGCAA+AGG + chr4.3:75502331-75502350 None:intergenic 45.0%
CCTTTGCAGTTCAACAGAGA+AGG - chr4.3:75502328-75502347 MS.gene064122:CDS 45.0%
CTCTTCATGTCACTCCAAGT+TGG - chr4.3:75502044-75502063 MS.gene064122:intron 45.0%
CTTGGATTCAAGGCTATCGA+AGG - chr4.3:75502369-75502388 MS.gene064122:CDS 45.0%
GACCAGCCATCTTTCTTTCA+TGG + chr4.3:75502744-75502763 None:intergenic 45.0%
GATCTTCCTTTCAAGAGCTC+CGG + chr4.3:75502709-75502728 None:intergenic 45.0%
GCCATCTCATGGAGGAAATA+TGG - chr4.3:75503398-75503417 MS.gene064122:CDS 45.0%
GCGAAACCAGTTCAAGAACT+TGG - chr4.3:75502535-75502554 MS.gene064122:CDS 45.0%
GCTATCGAAGGATCTTCCTT+CGG - chr4.3:75502381-75502400 MS.gene064122:CDS 45.0%
GCTGAGAGAATGCAAAGCAA+AGG - chr4.3:75502568-75502587 MS.gene064122:CDS 45.0%
GGTGGTGAAGAAGAAGTTAG+AGG + chr4.3:75500958-75500977 None:intergenic 45.0%
GTAACATCCATGGCAGTCAA+GGG + chr4.3:75502772-75502791 None:intergenic 45.0%
TCTTCAGAAGCTTCGATGCA+AGG - chr4.3:75502405-75502424 MS.gene064122:CDS 45.0%
TGCAAGAATTTATTGGGCCG+GGG - chr4.3:75503206-75503225 MS.gene064122:intron 45.0%
TGTTCTTGCCAATGATGTGC+GGG - chr4.3:75501964-75501983 MS.gene064122:intron 45.0%
TTATTCCTTCCAGGGTCACA+GGG - chr4.3:75503885-75503904 MS.gene064122:CDS 45.0%
TTGCAAGCAAAGGTTCCTAG+TGG - chr4.3:75501353-75501372 MS.gene064122:intron 45.0%
! ACACTTGATGACAAAGCCAG+TGG - chr4.3:75502009-75502028 MS.gene064122:intron 45.0%
! GACTTGGCGGATGAAGAAAA+GGG - chr4.3:75501380-75501399 MS.gene064122:intron 45.0%
! GTTTTCATGCCATCTCATGG+AGG - chr4.3:75503390-75503409 MS.gene064122:CDS 45.0%
! TGACTTGGCGGATGAAGAAA+AGG - chr4.3:75501379-75501398 MS.gene064122:intron 45.0%
! TTGATGACAAAGCCAGTGGA+GGG - chr4.3:75502013-75502032 MS.gene064122:intron 45.0%
!! CTTATGGTGGTTGTTTCTGG+AGG - chr4.3:75501509-75501528 MS.gene064122:intron 45.0%
!! TAGGATTCTTGGTGCTGCTT+TGG - chr4.3:75501577-75501596 MS.gene064122:CDS 45.0%
!!! GAGTTTTGGAAGCAACCAGA+TGG - chr4.3:75501404-75501423 MS.gene064122:intron 45.0%
AAATATGGGCCATGCGCTTC+AGG - chr4.3:75503413-75503432 MS.gene064122:CDS 50.0%
ACCTTGCTAGTCCTGAGTTC+CGG + chr4.3:75504042-75504061 None:intergenic 50.0%
AGTCGTTGCACATGCACTCT+GGG + chr4.3:75504092-75504111 None:intergenic 50.0%
CCGTTCGCGCTATCTCAAAA+GGG - chr4.3:75502069-75502088 MS.gene064122:intron 50.0%
CTTGGAGTGACATGAAGAGG+AGG + chr4.3:75502043-75502062 None:intergenic 50.0%
GAATGAGAGGAGAGAACGAC+CGG - chr4.3:75502687-75502706 MS.gene064122:intron 50.0%
GAGAGCTTGTGGGAATAAGC+TGG + chr4.3:75502935-75502954 None:intergenic 50.0%
GCAAGAATTTATTGGGCCGG+GGG - chr4.3:75503207-75503226 MS.gene064122:intron 50.0%
GGCTTATACCCTAACCCATC+TGG + chr4.3:75501422-75501441 None:intergenic 50.0%
GGTAACATCCATGGCAGTCA+AGG + chr4.3:75502773-75502792 None:intergenic 50.0%
GGTTCCTAGTGGAGTTGACT+TGG - chr4.3:75501364-75501383 MS.gene064122:intron 50.0%
GTTATTCCTTCCAGGGTCAC+AGG - chr4.3:75503884-75503903 MS.gene064122:CDS 50.0%
TCAAACTCAGGACTCAGACC+AGG + chr4.3:75502655-75502674 None:intergenic 50.0%
TCCGTTCGCGCTATCTCAAA+AGG - chr4.3:75502068-75502087 MS.gene064122:intron 50.0%
TCTTTGTCCCTTGACTGCCA+TGG - chr4.3:75502762-75502781 MS.gene064122:intron 50.0%
TGACTGCCATGGATGTTACC+AGG - chr4.3:75502773-75502792 MS.gene064122:intron 50.0%
TGGAAAAGGATGTGTGGGTG+AGG - chr4.3:75502617-75502636 MS.gene064122:intron 50.0%
! AACTCAGGACTAGCAAGGTC+GGG - chr4.3:75504043-75504062 MS.gene064122:CDS 50.0%
! CGAAGCTTCTGAAGATCCGA+AGG + chr4.3:75502400-75502419 None:intergenic 50.0%
! CTTGATGACAAAGCCAGTGG+AGG - chr4.3:75502012-75502031 MS.gene064122:intron 50.0%
! GAACTCAGGACTAGCAAGGT+CGG - chr4.3:75504042-75504061 MS.gene064122:CDS 50.0%
! TGGAAGCAACCAGATGGGTT+AGG - chr4.3:75501410-75501429 MS.gene064122:intron 50.0%
!! GGAAGCAACCAGATGGGTTA+GGG - chr4.3:75501411-75501430 MS.gene064122:intron 50.0%
!! TCCTAGTGGAGTTGACTTGG+CGG - chr4.3:75501367-75501386 MS.gene064122:intron 50.0%
!!! CCCTTTTGAGATAGCGCGAA+CGG + chr4.3:75502072-75502091 None:intergenic 50.0%
AAGGATGTGTGGGTGAGGAC+TGG - chr4.3:75502622-75502641 MS.gene064122:intron 55.0%
CATTAGGACAGCCGGAACTC+AGG - chr4.3:75504028-75504047 MS.gene064122:CDS 55.0%
CGAAACCACCCGCACATCAT+TGG + chr4.3:75501975-75501994 None:intergenic 55.0%
CTGGAGGGATGAATCAGCAG+AGG - chr4.3:75501525-75501544 MS.gene064122:CDS 55.0%
GAACGACCGGAGCTCTTGAA+AGG - chr4.3:75502700-75502719 MS.gene064122:intron 55.0%
GAGTCGTTGCACATGCACTC+TGG + chr4.3:75504093-75504112 None:intergenic 55.0%
TAGCGCGAACGGATCCAACT+TGG + chr4.3:75502061-75502080 None:intergenic 55.0%
TCCGCCAAGTCAACTCCACT+AGG + chr4.3:75501371-75501390 None:intergenic 55.0%
TCTTGCCAATGATGTGCGGG+TGG - chr4.3:75501967-75501986 MS.gene064122:intron 55.0%
!! GTGAGGACTGGTTGTCTACC+TGG - chr4.3:75502634-75502653 MS.gene064122:intron 55.0%
!! TTGCACATGCACTCTGGGAC+AGG + chr4.3:75504087-75504106 None:intergenic 55.0%
AGAGGAGGACTTCCCTCCAC+TGG + chr4.3:75502028-75502047 None:intergenic 60.0%
ATAGGCCCTGTGACCCTGGA+AGG + chr4.3:75503893-75503912 None:intergenic 60.0%
TCCACCCAATGGTTGTCCCC+CGG + chr4.3:75503226-75503245 None:intergenic 60.0%
! CAGGACTAGCAAGGTCGGGA+GGG - chr4.3:75504047-75504066 MS.gene064122:CDS 60.0%
! GCCGGAACTCAGGACTAGCA+AGG - chr4.3:75504038-75504057 MS.gene064122:CDS 60.0%
! TCAGGACTAGCAAGGTCGGG+AGG - chr4.3:75504046-75504065 MS.gene064122:CDS 60.0%
CAGGGTCACAGGGCCTATGC+AGG - chr4.3:75503895-75503914 MS.gene064122:CDS 65.0%
CTGCATAGGCCCTGTGACCC+TGG + chr4.3:75503897-75503916 None:intergenic 65.0%
TGGGCCGGGGGACAACCATT+GGG - chr4.3:75503219-75503238 MS.gene064122:intron 65.0%
TTGGGCCGGGGGACAACCAT+TGG - chr4.3:75503218-75503237 MS.gene064122:intron 65.0%
GCCGGGGGACAACCATTGGG+TGG - chr4.3:75503222-75503241 MS.gene064122:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75500819 75504128 75500819 ID=MS.gene064122
chr4.3 mRNA 75500819 75504128 75500819 ID=MS.gene064122.t1;Parent=MS.gene064122
chr4.3 exon 75503305 75504128 75503305 ID=MS.gene064122.t1.exon1;Parent=MS.gene064122.t1
chr4.3 CDS 75503305 75504128 75503305 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
chr4.3 exon 75502857 75503022 75502857 ID=MS.gene064122.t1.exon2;Parent=MS.gene064122.t1
chr4.3 CDS 75502857 75503022 75502857 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
chr4.3 exon 75502521 75502610 75502521 ID=MS.gene064122.t1.exon3;Parent=MS.gene064122.t1
chr4.3 CDS 75502521 75502610 75502521 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
chr4.3 exon 75502153 75502436 75502153 ID=MS.gene064122.t1.exon4;Parent=MS.gene064122.t1
chr4.3 CDS 75502153 75502436 75502153 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
chr4.3 exon 75501513 75501771 75501513 ID=MS.gene064122.t1.exon5;Parent=MS.gene064122.t1
chr4.3 CDS 75501513 75501771 75501513 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
chr4.3 exon 75500819 75501049 75500819 ID=MS.gene064122.t1.exon6;Parent=MS.gene064122.t1
chr4.3 CDS 75500819 75501049 75500819 ID=cds.MS.gene064122.t1;Parent=MS.gene064122.t1
Gene Sequence

>MS.gene064122

ATGGCTAAGTCAAAGAACAATGCAAAGAAACTCTCATACATCTCAGTTCCTTCACAAATAATCAATTCCATTTCATCTTCTTCTATTCAATCATTACTTGATGATCAAACTCCAAAGAAATCTTCAACAAAAATTGCCTCTAACTTCTTCTTCACCACCTTAACAAAATGGAAAAACCCTTCAAGATTGTTCTTCTTTTTCACACTCTTATTTCTTGGTGTTTTTGCTTTGTTTATGATTGGTTTCAATCTTAAAACACCGTTTTTTAAAACCCCATGTGCTACAAATGTTGAAAAGCCCTTAATCTCAAATGGGTTTGGTTCAAAATCTGAACTTAAGGTTGTTTCAATGAATAAAAATGAGATTTTTAATGGTGGTGTTTTGGTAAAATCTGAACTTGGGGTTGTTCAAAACCCTTCAATCTCAAATGGGTTTGATTCAAAATCTGAACTTGGTGTTGTTTCAATGAATAAAGATGAGGTTTTGAATAAAGGTAGTTTGTCAAATTCACTTCCTTTGATTGGTAATGTAATGTTGCAAGCAAAGGTTCCTAGTGGAGTTGACTTGGCGGATGAAGAAAAGGGTGAGTTTTGGAAGCAACCAGATGGGTTAGGGTATAAGCCTTGTTTGGATTTCAGTAGGGATTACAGAAGAGAAAGTGATGGGATTGTTAAGAATAGAAAGAGGTATCTTATGGTGGTTGTTTCTGGAGGGATGAATCAGCAGAGGAATCAAATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTGCTTTGGTTGTTCCTATTTTGCAAGTTAATGTCATCTGGGGAGATGAAAGGTAAACTATACTATACTATTCTTGTTCATGGTTTGTGATATTGTGGTGATGATTTTAATATATGAAGATTTATGCAAGGTTCAGTTGTTACAATTTTGGACTTAAATCTTCCTAAGGACCAATATTGGAACATAATCTAAGCCTAAGCATAGTTTTTCCTTGATACTGGTTATGTTTCGTTGTTAATGTTGATTAAATTTCTTTCCTTATTTGTGAATGCTAGAAGAGAGTTTTCTGTAATTGATTATGTTTTTCCTTATTGATTTTGGTTTTGTTTTGCAGTGAATTTGCTGATATATTTGATTTTGATCACTTCAAGAATGTTCTTGCCAATGATGTGCGGGTGGTTTCGTCATTGCCATCAACACACTTGATGACAAAGCCAGTGGAGGGAAGTCCTCCTCTTCATGTCACTCCAAGTTGGATCCGTTCGCGCTATCTCAAAAGGGTAAGATTTTTTTGTTAATTACTCATGTTTTTGCATCTTTAGGTTTCAGTCCTAAGTTGTTGCTAAGTTTTTGTCTACTTTTGCTTTGAAAAATTTCAGACAATGATTGTCCCTTTGATGAGAAAGTGTGTTAACTTGTATAAATCATGTTAACTAATGAAAGCTTTGACTTCTGTGGAAATAAAAATTTGGGCAGTGAGTGATTGTTGAGCTTGAGTTTAACATGGTCTTTGTTGTCCTTTGCAGTTCAACAGAGAAGGTGTTTTGCTTTTACGCAGCTTGGATTCAAGGCTATCGAAGGATCTTCCTTCGGATCTTCAGAAGCTTCGATGCAAGGTACTGTTAAGTCTCTATAGTATCTTCATTTGTGTAACAATCGGTAGCATATTCTACATGTTCTCATCTCACCTATGTTTTCAGGTTGCTTTTAGTGCATTGAGATTTGCGAAACCAGTTCAAGAACTTGGAAACAAGATTGCTGAGAGAATGCAAAGCAAAGGACCCTACCTTGCTCTTCATCTAAGAATGGAAAAGGATGTGTGGGTGAGGACTGGTTGTCTACCTGGTCTGAGTCCTGAGTTTGACGAGATTGTAAAGAATGAGAGGAGAGAACGACCGGAGCTCTTGAAAGGAAGATCAAACATGACTTACCATGAAAGAAAGATGGCTGGTCTTTGTCCCTTGACTGCCATGGATGTTACCAGGTAAATATTAAATTCTTCTACTTTTGTATAGGAGTCTGTTTGGATTCTCTTATTTGAGTTTATTACTGGCACTTACACTTATGAAACTATTTGGGAGAGCTTATGGAAACAACTTATGACATGTCCATAAACTGTTTCCAGCTTATTCCCACAAGCTCTCCAAGATAGCTTATGAAATCAGTTTAGTTTATATAAAAATAGTTCGATTTTATTTTATCTTTTATTATAGAAATAGCGTATACATAAATACTTATATGATAAACACTTATGCTACAAGCGCTTAATTAAGTTGTTTATCCAAAGATCCTATAGGCTTCACTTTTCAGTGGTTTTCTGAATATTAGTGTGACAGAGTTTTGTTTATTTGTTTTGGATTTATAGGTTGCTTAAAGCTTTAGGAGCTCAAAAAGATGCAAGAATTTATTGGGCCGGGGGACAACCATTGGGTGGAAAAGAAGCTTTGCATCCATTAATCCATGAGTTTCCACATCTTTATAATAAGGAAGATCTTGCATTGCCTGGAGAACTAGAACCATTTTCGAAGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTTTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCATGCGCTTCAGGTATGACTATATAATGACAGCAAGAGTGCTTTGCAAATAAGATACTCACATAAGCATTGATGATTGTTGCATAACTATATTGTAATCATGCTTACATATATTGGCCATGCTTTCATATATACTGTATTCACACATATTTTATAAGAAAAGATTGCGACCTTTCAACTTTAGTCCCTATAAAATTTGTACTCACCTTTAGTCTTCATATTTAATAAAAATAGTGTGCTTTTAGTAATTAAAAAGGACTGATGATACAATCAATTATTTTAGGGATGTTGATGGGTCGTGATTTTATAGGACCAAAAACATATTTTCTCCATAAATTGTAATCATGCAATTAAGATAGTCACATTGATGGATGTTGCATAAATATATTGTAAATGTAATATTGCATACGCATATTTAATGTAGATTCTTCATTTGACAATTTCTAATATCTGAACCTAAATGTTATTCCTTCCAGGGTCACAGGGCCTATGCAGGACACAAGAAATACATAACCCCAAACAAAAGACAAATGCATCCCTATTTTCTGAATTCTTCTCTCCCTGAAGCAGAGTTTAACAGCATTGTCAAAGAATTGCATCAGAATTCATTAGGACAGCCGGAACTCAGGACTAGCAAGGTCGGGAGGGATGTCACGAAGTATCCTGTCCCAGAGTGCATGTGCAACGACTCACATAATGATTCGTAG
Protein sequence

>MS.gene064122.t1

MAKSKNNAKKLSYISVPSQIINSISSSSIQSLLDDQTPKKSSTKIASNFFFTTLTKWKNPSRLFFFFTLLFLGVFALFMIGFNLKTPFFKTPCATNVEKPLISNGFGSKSELKVVSMNKNEIFNGGVLVKSELGVVQNPSISNGFDSKSELGVVSMNKDEVLNKGSLSNSLPLIGNVMLQAKVPSGVDLADEEKGEFWKQPDGLGYKPCLDFSRDYRRESDGIVKNRKRYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFADIFDFDHFKNVLANDVRVVSSLPSTHLMTKPVEGSPPLHVTPSWIRSRYLKRFNREGVLLLRSLDSRLSKDLPSDLQKLRCKVAFSALRFAKPVQELGNKIAERMQSKGPYLALHLRMEKDVWVRTGCLPGLSPEFDEIVKNERRERPELLKGRSNMTYHERKMAGLCPLTAMDVTRLLKALGAQKDARIYWAGGQPLGGKEALHPLIHEFPHLYNKEDLALPGELEPFSKKASLMAAIDYIVSEKSDVFMPSHGGNMGHALQGHRAYAGHKKYITPNKRQMHPYFLNSSLPEAEFNSIVKELHQNSLGQPELRTSKVGRDVTKYPVPECMCNDSHNDS