Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064192.t1 | AES98673.1 | 45.7 | 188 | 102 | 0 | 1 | 188 | 1 | 188 | 6.70E-40 | 174.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064192.t1 | G7JZK4 | 45.7 | 188 | 102 | 0 | 1 | 188 | 1 | 188 | 4.8e-40 | 174.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene064192 | 0.816255 | 6.08E-52 | -1.69E-46 |
| MS.gene054484 | MS.gene064192 | 0.83137 | 1.73E-55 | -1.69E-46 |
| MS.gene058580 | MS.gene064192 | 0.803432 | 3.50E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene064192 | 0.804443 | 2.15E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064192.t1 | MTR_5g071410 | 48.404 | 188 | 97 | 0 | 1 | 188 | 1 | 188 | 6.70e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAAACTGCCAAAGAGTT+TGG | 0.271815 | 4.2:+56162256 | MS.gene064192:CDS |
| GTTCTCGAAACAAGCTATAA+AGG | 0.296909 | 4.2:+56161585 | MS.gene064192:CDS |
| AAAACACTCGGAATGTAAAT+TGG | 0.334288 | 4.2:-56162352 | None:intergenic |
| TACGAGTTTGTTACCTGGTC+TGG | 0.346210 | 4.2:-56161634 | None:intergenic |
| ATCGTCTAAAATAATATCAA+TGG | 0.402476 | 4.2:-56162398 | None:intergenic |
| AAATTCTCGAAACTATTGTT+TGG | 0.414979 | 4.2:+56161611 | MS.gene064192:CDS |
| GGATTGCTTCTTTATGATGA+AGG | 0.418452 | 4.2:+56162175 | MS.gene064192:CDS |
| GATGGTAGATTTATTAAGAA+TGG | 0.429272 | 4.2:-56162604 | None:intergenic |
| GAAATCAACTTCAACTCTCC+TGG | 0.432821 | 4.2:+56162154 | MS.gene064192:CDS |
| CTCATAGTCAAGCCTAATCT+TGG | 0.441071 | 4.2:-56162293 | None:intergenic |
| TTCTCGAAACAAGCTATAAA+GGG | 0.459978 | 4.2:+56161586 | MS.gene064192:CDS |
| ATTAACTATGTCACACACTT+TGG | 0.464937 | 4.2:+56162199 | MS.gene064192:CDS |
| CAAGATTAGGCTTGACTATG+AGG | 0.465345 | 4.2:+56162294 | MS.gene064192:CDS |
| TGTAGTAGTGTTGTCGAATA+CGG | 0.470976 | 4.2:-56162117 | None:intergenic |
| TTGTTGACAACACCTGCATT+TGG | 0.472847 | 4.2:-56162481 | None:intergenic |
| ACTGACACTAAACAAACAAA+TGG | 0.477164 | 4.2:+56162424 | MS.gene064192:CDS |
| AACTATTGTTTGGCCAGACC+AGG | 0.481625 | 4.2:+56161621 | MS.gene064192:CDS |
| GGATGACATAGGGGTGTACA+TGG | 0.487117 | 4.2:+56162225 | MS.gene064192:CDS |
| GGTTCTTCTTTAACTCGTGC+GGG | 0.488642 | 4.2:-56162376 | None:intergenic |
| TTTGACGTAACAAGGCTTGT+TGG | 0.490445 | 4.2:-56162579 | None:intergenic |
| TCGTCTAAAATAATATCAAT+GGG | 0.502312 | 4.2:-56162397 | None:intergenic |
| TCATCATAAAGAAGCAATCC+AGG | 0.515667 | 4.2:-56162172 | None:intergenic |
| ACTTTGGCATGGATGACATA+GGG | 0.518231 | 4.2:+56162215 | MS.gene064192:CDS |
| ATAGGGGTGTACATGGGTAA+TGG | 0.523281 | 4.2:+56162232 | MS.gene064192:CDS |
| ATGTATAAGCTGACACAACT+AGG | 0.527948 | 4.2:+56162328 | MS.gene064192:CDS |
| AAATGGATGTTGATTCCGAC+AGG | 0.528326 | 4.2:+56160949 | None:intergenic |
| GGGTTCTTCTTTAACTCGTG+CGG | 0.537410 | 4.2:-56162377 | None:intergenic |
| AATGAAATTCAGCCAAATGC+AGG | 0.538870 | 4.2:+56162469 | MS.gene064192:CDS |
| AGTTGAAGTTGATTTCTTTG+CGG | 0.550393 | 4.2:-56162147 | None:intergenic |
| CTATGTCACACACTTTGGCA+TGG | 0.550418 | 4.2:+56162204 | MS.gene064192:CDS |
| TAGTCAAGCCTAATCTTGGT+TGG | 0.563870 | 4.2:-56162289 | None:intergenic |
| CACTTTGGCATGGATGACAT+AGG | 0.572562 | 4.2:+56162214 | MS.gene064192:CDS |
| GCATTATCATCACTTTGAGA+AGG | 0.593701 | 4.2:+56162525 | MS.gene064192:CDS |
| AAGATTAGGCTTGACTATGA+GGG | 0.603523 | 4.2:+56162295 | MS.gene064192:CDS |
| ACTCGTGCGGGGAAAACACT+CGG | 0.607382 | 4.2:-56162364 | None:intergenic |
| CACTAAACAAACAAATGGTG+AGG | 0.608157 | 4.2:+56162429 | MS.gene064192:CDS |
| TATTCGACAACACTACTACA+AGG | 0.634552 | 4.2:+56162120 | MS.gene064192:CDS |
| CAATTTGCAAAGAACTGATG+CGG | 0.646476 | 4.2:+56162501 | MS.gene064192:CDS |
| GTTCTTCTTTAACTCGTGCG+GGG | 0.667996 | 4.2:-56162375 | None:intergenic |
| CTTTGGCATGGATGACATAG+GGG | 0.676461 | 4.2:+56162216 | MS.gene064192:CDS |
| GATGACATAGGGGTGTACAT+GGG | 0.697797 | 4.2:+56162226 | MS.gene064192:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTGAAACTTATAATTACA+TGG | + | chr4.2:56161163-56161182 | MS.gene064192:intron | 15.0% |
| !!! | TTCTGATTAAATTTTTTAAG+AGG | - | chr4.2:56161498-56161517 | None:intergenic | 15.0% |
| !! | AAACTTATAATTACATGGTT+AGG | + | chr4.2:56161168-56161187 | MS.gene064192:intron | 20.0% |
| !! | AAATTGACACAAGAATAAAA+GGG | - | chr4.2:56161530-56161549 | None:intergenic | 20.0% |
| !! | AACTTATAATTACATGGTTA+GGG | + | chr4.2:56161169-56161188 | MS.gene064192:intron | 20.0% |
| !! | ATAATGATGAAATAGAAATG+TGG | + | chr4.2:56161264-56161283 | MS.gene064192:intron | 20.0% |
| !! | ATGAAATGATATTGAAATGT+TGG | - | chr4.2:56161070-56161089 | None:intergenic | 20.0% |
| !! | TAAATTGACACAAGAATAAA+AGG | - | chr4.2:56161531-56161550 | None:intergenic | 20.0% |
| !!! | ATCGTCTAAAATAATATCAA+TGG | - | chr4.2:56162401-56162420 | None:intergenic | 20.0% |
| !!! | ATTTTTTGTTTCAATGTAGA+TGG | + | chr4.2:56161563-56161582 | MS.gene064192:intron | 20.0% |
| !!! | TATTTTACAATAGTAGATGA+TGG | - | chr4.2:56162625-56162644 | None:intergenic | 20.0% |
| !!! | TCGTCTAAAATAATATCAAT+GGG | - | chr4.2:56162400-56162419 | None:intergenic | 20.0% |
| !!! | TTTATTTTCTTGATGAATGT+TGG | + | chr4.2:56161724-56161743 | MS.gene064192:intron | 20.0% |
| ! | AAATTCTCGAAACTATTGTT+TGG | + | chr4.2:56161611-56161630 | MS.gene064192:CDS | 25.0% |
| ! | AGATTACTGGTAATTTCTTT+AGG | + | chr4.2:56161807-56161826 | MS.gene064192:intron | 25.0% |
| ! | GATGGTAGATTTATTAAGAA+TGG | - | chr4.2:56162607-56162626 | None:intergenic | 25.0% |
| ! | TAGAATTTAACTCCAACAAT+TGG | - | chr4.2:56161959-56161978 | None:intergenic | 25.0% |
| !! | AGGAATAAAAGTATATGTGA+TGG | - | chr4.2:56161478-56161497 | None:intergenic | 25.0% |
| !! | TCAATGATTGAGTTTTTGTT+TGG | - | chr4.2:56160987-56161006 | None:intergenic | 25.0% |
| !!! | AAAAGATATTTTGACGGAAA+AGG | + | chr4.2:56161419-56161438 | MS.gene064192:intron | 25.0% |
| !!! | AAAGATATTTTGACGGAAAA+GGG | + | chr4.2:56161420-56161439 | MS.gene064192:intron | 25.0% |
| !!! | GTGCAAAAAAGATATTTTGA+CGG | + | chr4.2:56161413-56161432 | MS.gene064192:intron | 25.0% |
| !!! | TCAGCTTATACATATTTTTG+AGG | - | chr4.2:56162321-56162340 | None:intergenic | 25.0% |
| AAAACACTCGGAATGTAAAT+TGG | - | chr4.2:56162355-56162374 | None:intergenic | 30.0% | |
| AACCAAAAATCCAAACTCTT+TGG | - | chr4.2:56162269-56162288 | None:intergenic | 30.0% | |
| ACCATAAAATCTAGATACAG+TGG | - | chr4.2:56161030-56161049 | None:intergenic | 30.0% | |
| ACTGACACTAAACAAACAAA+TGG | + | chr4.2:56162424-56162443 | MS.gene064192:CDS | 30.0% | |
| ATTAACTATGTCACACACTT+TGG | + | chr4.2:56162199-56162218 | MS.gene064192:CDS | 30.0% | |
| GAAATTACCAGTAATCTTAC+TGG | - | chr4.2:56161804-56161823 | None:intergenic | 30.0% | |
| TTAGGGTTTCATAATCTGAT+GGG | + | chr4.2:56161186-56161205 | MS.gene064192:intron | 30.0% | |
| TTCTCGAAACAAGCTATAAA+GGG | + | chr4.2:56161586-56161605 | MS.gene064192:CDS | 30.0% | |
| ! | AGTTGAAGTTGATTTCTTTG+CGG | - | chr4.2:56162150-56162169 | None:intergenic | 30.0% |
| ! | GTGATGGAATTTGTACTTAT+AGG | - | chr4.2:56161462-56161481 | None:intergenic | 30.0% |
| ! | TCCACTGTATCTAGATTTTA+TGG | + | chr4.2:56161026-56161045 | MS.gene064192:intron | 30.0% |
| ! | TTTGAAACCAGTAAGATTAC+TGG | + | chr4.2:56161794-56161813 | MS.gene064192:intron | 30.0% |
| !! | TCTAAGTTTGCTTGATTTTC+TGG | + | chr4.2:56161907-56161926 | MS.gene064192:intron | 30.0% |
| !!! | TAGCTGTTGTTTTCTGTTTA+TGG | + | chr4.2:56161859-56161878 | MS.gene064192:intron | 30.0% |
| AAGATTAGGCTTGACTATGA+GGG | + | chr4.2:56162295-56162314 | MS.gene064192:CDS | 35.0% | |
| AATGAAATTCAGCCAAATGC+AGG | + | chr4.2:56162469-56162488 | MS.gene064192:CDS | 35.0% | |
| ACAGAAAACAACAGCTATAG+CGG | - | chr4.2:56161856-56161875 | None:intergenic | 35.0% | |
| AGCCTACATTGACGTAAAAA+AGG | - | chr4.2:56162092-56162111 | None:intergenic | 35.0% | |
| AGTTGAGAATGATGATTTCC+AGG | - | chr4.2:56161135-56161154 | None:intergenic | 35.0% | |
| ATACTGCTACTGCAGAATTT+AGG | + | chr4.2:56161230-56161249 | MS.gene064192:intron | 35.0% | |
| ATCAAAACTGCCAAAGAGTT+TGG | + | chr4.2:56162256-56162275 | MS.gene064192:CDS | 35.0% | |
| ATGTATAAGCTGACACAACT+AGG | + | chr4.2:56162328-56162347 | MS.gene064192:CDS | 35.0% | |
| CAATTTGCAAAGAACTGATG+CGG | + | chr4.2:56162501-56162520 | MS.gene064192:CDS | 35.0% | |
| CACTAAACAAACAAATGGTG+AGG | + | chr4.2:56162429-56162448 | MS.gene064192:CDS | 35.0% | |
| CAGAGTATAAAGCCAATTGT+TGG | + | chr4.2:56161944-56161963 | MS.gene064192:intron | 35.0% | |
| GCCTACATTGACGTAAAAAA+GGG | - | chr4.2:56162091-56162110 | None:intergenic | 35.0% | |
| GGATTGCTTCTTTATGATGA+AGG | + | chr4.2:56162175-56162194 | MS.gene064192:CDS | 35.0% | |
| GTTAGAAGTGAGGTAAAGAT+GGG | + | chr4.2:56161286-56161305 | MS.gene064192:intron | 35.0% | |
| GTTAGGGTTTCATAATCTGA+TGG | + | chr4.2:56161185-56161204 | MS.gene064192:intron | 35.0% | |
| GTTCTCGAAACAAGCTATAA+AGG | + | chr4.2:56161585-56161604 | MS.gene064192:CDS | 35.0% | |
| TAGAAATGTGGTTAGAAGTG+AGG | + | chr4.2:56161276-56161295 | MS.gene064192:intron | 35.0% | |
| TATTCGACAACACTACTACA+AGG | + | chr4.2:56162120-56162139 | MS.gene064192:CDS | 35.0% | |
| TCATCATAAAGAAGCAATCC+AGG | - | chr4.2:56162175-56162194 | None:intergenic | 35.0% | |
| TGGAAATGAAACATACCTGT+CGG | - | chr4.2:56160967-56160986 | None:intergenic | 35.0% | |
| TGTAGTAGTGTTGTCGAATA+CGG | - | chr4.2:56162120-56162139 | None:intergenic | 35.0% | |
| TTAGAAGTGAGGTAAAGATG+GGG | + | chr4.2:56161287-56161306 | MS.gene064192:intron | 35.0% | |
| ! | GAATGGTTTTTGACGTAACA+AGG | - | chr4.2:56162590-56162609 | None:intergenic | 35.0% |
| ! | GCATTATCATCACTTTGAGA+AGG | + | chr4.2:56162525-56162544 | MS.gene064192:CDS | 35.0% |
| ! | TGCCAAAGAGTTTGGATTTT+TGG | + | chr4.2:56162264-56162283 | MS.gene064192:CDS | 35.0% |
| ! | TTTTACGTCAATGTAGGCTA+GGG | + | chr4.2:56162093-56162112 | MS.gene064192:intron | 35.0% |
| ! | TTTTTACGTCAATGTAGGCT+AGG | + | chr4.2:56162092-56162111 | MS.gene064192:intron | 35.0% |
| !!! | ACCCTTTTTTACGTCAATGT+AGG | + | chr4.2:56162087-56162106 | MS.gene064192:intron | 35.0% |
| !!! | TTTTGGTTCCAACCAAGATT+AGG | + | chr4.2:56162281-56162300 | MS.gene064192:CDS | 35.0% |
| ACTTTGGCATGGATGACATA+GGG | + | chr4.2:56162215-56162234 | MS.gene064192:CDS | 40.0% | |
| CAAGATTAGGCTTGACTATG+AGG | + | chr4.2:56162294-56162313 | MS.gene064192:CDS | 40.0% | |
| CTCATAGTCAAGCCTAATCT+TGG | - | chr4.2:56162296-56162315 | None:intergenic | 40.0% | |
| GAAATCAACTTCAACTCTCC+TGG | + | chr4.2:56162154-56162173 | MS.gene064192:CDS | 40.0% | |
| GGTTAGAAGTGAGGTAAAGA+TGG | + | chr4.2:56161285-56161304 | MS.gene064192:intron | 40.0% | |
| TAGTCAAGCCTAATCTTGGT+TGG | - | chr4.2:56162292-56162311 | None:intergenic | 40.0% | |
| TTGTTGACAACACCTGCATT+TGG | - | chr4.2:56162484-56162503 | None:intergenic | 40.0% | |
| TTTGACGTAACAAGGCTTGT+TGG | - | chr4.2:56162582-56162601 | None:intergenic | 40.0% | |
| !! | GTTTCTACGAGTTTGTTACC+TGG | - | chr4.2:56161642-56161661 | None:intergenic | 40.0% |
| !!! | CAAGGCTTGTTGGTTTTCAA+TGG | - | chr4.2:56162572-56162591 | None:intergenic | 40.0% |
| AACTATTGTTTGGCCAGACC+AGG | + | chr4.2:56161621-56161640 | MS.gene064192:CDS | 45.0% | |
| ACAGAAAGGACATGCATGCA+TGG | - | chr4.2:56161103-56161122 | None:intergenic | 45.0% | |
| AGAAAGCAGAAACGCAGCAA+AGG | - | chr4.2:56161332-56161351 | None:intergenic | 45.0% | |
| CACTTTGGCATGGATGACAT+AGG | + | chr4.2:56162214-56162233 | MS.gene064192:CDS | 45.0% | |
| CCTTTCTGTTCACTTCAACC+TGG | + | chr4.2:56161114-56161133 | MS.gene064192:intron | 45.0% | |
| CTATGTCACACACTTTGGCA+TGG | + | chr4.2:56162204-56162223 | MS.gene064192:CDS | 45.0% | |
| CTTTGGCATGGATGACATAG+GGG | + | chr4.2:56162216-56162235 | MS.gene064192:CDS | 45.0% | |
| GGGTTCTTCTTTAACTCGTG+CGG | - | chr4.2:56162380-56162399 | None:intergenic | 45.0% | |
| GGTTCTTCTTTAACTCGTGC+GGG | - | chr4.2:56162379-56162398 | None:intergenic | 45.0% | |
| GTTCTTCTTTAACTCGTGCG+GGG | - | chr4.2:56162378-56162397 | None:intergenic | 45.0% | |
| ! | ATAGGGGTGTACATGGGTAA+TGG | + | chr4.2:56162232-56162251 | MS.gene064192:CDS | 45.0% |
| ! | CCAGGTTGAAGTGAACAGAA+AGG | - | chr4.2:56161117-56161136 | None:intergenic | 45.0% |
| ! | GATGACATAGGGGTGTACAT+GGG | + | chr4.2:56162226-56162245 | MS.gene064192:CDS | 45.0% |
| !! | TACGAGTTTGTTACCTGGTC+TGG | - | chr4.2:56161637-56161656 | None:intergenic | 45.0% |
| !!! | GGCTTGTTGGTTTTCAATGG+AGG | - | chr4.2:56162569-56162588 | None:intergenic | 45.0% |
| GAAGTGAGGTAAAGATGGGG+AGG | + | chr4.2:56161290-56161309 | MS.gene064192:intron | 50.0% | |
| ! | GGATGACATAGGGGTGTACA+TGG | + | chr4.2:56162225-56162244 | MS.gene064192:CDS | 50.0% |
| ACTCGTGCGGGGAAAACACT+CGG | - | chr4.2:56162367-56162386 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 56160951 | 56162645 | 56160951 | ID=MS.gene064192 |
| chr4.2 | mRNA | 56160951 | 56162645 | 56160951 | ID=MS.gene064192.t1;Parent=MS.gene064192 |
| chr4.2 | exon | 56160951 | 56160970 | 56160951 | ID=MS.gene064192.t1.exon1;Parent=MS.gene064192.t1 |
| chr4.2 | CDS | 56160951 | 56160970 | 56160951 | ID=cds.MS.gene064192.t1;Parent=MS.gene064192.t1 |
| chr4.2 | exon | 56161582 | 56161642 | 56161582 | ID=MS.gene064192.t1.exon2;Parent=MS.gene064192.t1 |
| chr4.2 | CDS | 56161582 | 56161642 | 56161582 | ID=cds.MS.gene064192.t1;Parent=MS.gene064192.t1 |
| chr4.2 | exon | 56162109 | 56162645 | 56162109 | ID=MS.gene064192.t1.exon3;Parent=MS.gene064192.t1 |
| chr4.2 | CDS | 56162109 | 56162645 | 56162109 | ID=cds.MS.gene064192.t1;Parent=MS.gene064192.t1 |
| Gene Sequence |
| Protein sequence |
