Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064754.t1 | XP_003603750.2 | 96.8 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 7.50E-90 | 340.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064754.t1 | F4I0N3 | 36.7 | 218 | 95 | 8 | 8 | 210 | 1 | 190 | 1.3e-18 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064754.t1 | G7J4R0 | 96.8 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 5.4e-90 | 340.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055371 | MS.gene064754 | 0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene064754 | 0.842145 | 3.09E-58 | -1.69E-46 |
| MS.gene058912 | MS.gene064754 | 0.840339 | 9.23E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064754.t1 | MTR_3g113810 | 96.774 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 1.46e-152 | 422 |
| MS.gene064754.t1 | MTR_5g021990 | 45.946 | 222 | 102 | 10 | 1 | 216 | 1 | 210 | 3.41e-46 | 152 |
| MS.gene064754.t1 | MTR_4g040170 | 96.970 | 66 | 2 | 0 | 152 | 217 | 1 | 66 | 2.23e-42 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064754.t1 | AT1G75720 | 43.519 | 216 | 83 | 8 | 8 | 210 | 1 | 190 | 2.78e-36 | 126 |
| MS.gene064754.t1 | AT1G75720 | 43.981 | 216 | 84 | 9 | 8 | 210 | 1 | 192 | 7.35e-36 | 125 |
| MS.gene064754.t1 | AT3G51220 | 35.096 | 208 | 100 | 7 | 15 | 212 | 4 | 186 | 4.09e-27 | 102 |
| MS.gene064754.t1 | AT2G17940 | 33.163 | 196 | 120 | 4 | 16 | 205 | 13 | 203 | 5.87e-23 | 92.4 |
| MS.gene064754.t1 | AT1G75720 | 43.791 | 153 | 57 | 6 | 71 | 210 | 12 | 148 | 1.70e-20 | 84.3 |
Find 38 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTCTATCTCTGTATTTC+TGG | 0.104808 | 3.4:+97835826 | None:intergenic |
| CATAATGGAGCAAATGAAAA+TGG | 0.193439 | 3.4:-97834830 | MS.gene064754:CDS |
| TAAAGAAGAAAGCATGTTAA+TGG | 0.275611 | 3.4:-97834735 | MS.gene064754:CDS |
| TCATTCATCATGGAACTTCT+TGG | 0.313646 | 3.4:+97834351 | None:intergenic |
| TGGAAAGGCACCCTTCTCTT+AGG | 0.324014 | 3.4:-97834439 | MS.gene064754:CDS |
| AAAGAGGCTGTTACATTATT+CGG | 0.326753 | 3.4:-97835782 | MS.gene064754:CDS |
| TAGACACAAGGGCGCCATTC+AGG | 0.343210 | 3.4:-97835811 | MS.gene064754:CDS |
| TCGTGCGTTCGAGTTCTTCC+TGG | 0.369083 | 3.4:+97834693 | None:intergenic |
| AAGTCATTAATTCCACTTAT+CGG | 0.388786 | 3.4:-97834404 | MS.gene064754:CDS |
| ATAATGGAGCAAATGAAAAT+GGG | 0.406872 | 3.4:-97834829 | MS.gene064754:CDS |
| TTCTTCTTCTTCCTAAGAGA+AGG | 0.423924 | 3.4:+97834428 | None:intergenic |
| TGATGGAGTTTCAAAAGAAA+AGG | 0.446231 | 3.4:-97834535 | MS.gene064754:CDS |
| TCATCAAGGTGTTGAGAAGT+TGG | 0.449442 | 3.4:-97834459 | MS.gene064754:CDS |
| AGGTAACATGACATGAGAAA+TGG | 0.454194 | 3.4:+97834487 | None:intergenic |
| ATTTGATGAAGAATTAATGA+TGG | 0.459950 | 3.4:-97834552 | MS.gene064754:CDS |
| TCTAATCTATAGATTCATAA+TGG | 0.462589 | 3.4:-97834845 | MS.gene064754:intron |
| TCTTCTTCTTCCTAAGAGAA+GGG | 0.487269 | 3.4:+97834429 | None:intergenic |
| ATGGGGTTGATCAGTCTTCA+AGG | 0.494065 | 3.4:-97834811 | MS.gene064754:CDS |
| TAATGTAACAGCCTCTTTGA+CGG | 0.499377 | 3.4:+97835787 | None:intergenic |
| TGAAAACGTTACAGCTGAGC+TGG | 0.505652 | 3.4:-97834786 | MS.gene064754:CDS |
| ACATTGTATGTCATCTCTCC+AGG | 0.522311 | 3.4:-97834711 | MS.gene064754:CDS |
| AAAGATGAATAAGAAGAAAG+AGG | 0.535423 | 3.4:-97835864 | None:intergenic |
| GCCTCTTTGACGGACCTGAA+TGG | 0.535948 | 3.4:+97835797 | None:intergenic |
| AAGATGAATAAGAAGAAAGA+GGG | 0.553271 | 3.4:-97835863 | MS.gene064754:CDS |
| GCCATTCAGGTCCGTCAAAG+AGG | 0.580083 | 3.4:-97835798 | MS.gene064754:CDS |
| ATGTTACCTCAAGGTCATCA+AGG | 0.584789 | 3.4:-97834473 | MS.gene064754:CDS |
| TCTCATGTCATGTTACCTCA+AGG | 0.592396 | 3.4:-97834482 | MS.gene064754:CDS |
| TGGGGTTGATCAGTCTTCAA+GGG | 0.595004 | 3.4:-97834810 | MS.gene064754:CDS |
| TCAACACCTTGATGACCTTG+AGG | 0.616815 | 3.4:+97834467 | None:intergenic |
| AAGGTGTTGAGAAGTTGGAA+AGG | 0.636189 | 3.4:-97834454 | MS.gene064754:CDS |
| TAATGGAGCAAATGAAAATG+GGG | 0.636381 | 3.4:-97834828 | MS.gene064754:CDS |
| TCATTAATTCCACTTATCGG+AGG | 0.648652 | 3.4:-97834401 | MS.gene064754:CDS |
| TGCAACTAAACTCAAACACC+AGG | 0.653524 | 3.4:-97835726 | MS.gene064754:intron |
| AAACGTTACAGCTGAGCTGG+AGG | 0.666569 | 3.4:-97834783 | MS.gene064754:CDS |
| ATGACATGAGAAATGGACGG+TGG | 0.709324 | 3.4:+97834494 | None:intergenic |
| GAAATACAGAGATAGACACA+AGG | 0.731685 | 3.4:-97835823 | MS.gene064754:CDS |
| AAATACAGAGATAGACACAA+GGG | 0.731958 | 3.4:-97835822 | MS.gene064754:CDS |
| AACATGACATGAGAAATGGA+CGG | 0.745302 | 3.4:+97834491 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAATTATTAATAATGTG+TGG | + | chr3.4:97835334-97835353 | None:intergenic | 10.0% |
| !! | ATAAAAATATAATCATCTAA+TGG | + | chr3.4:97834760-97834779 | None:intergenic | 10.0% |
| !! | ATTATAATATGTTTGTAAGT+GGG | - | chr3.4:97834674-97834693 | MS.gene064754:CDS | 15.0% |
| !!! | ATTTTATTAGAGTATCAAAA+TGG | - | chr3.4:97834539-97834558 | MS.gene064754:CDS | 15.0% |
| !! | AAATTTAATTATTGATCCGT+AGG | - | chr3.4:97835136-97835155 | MS.gene064754:intron | 20.0% |
| !! | AATAAAATTGAATGATGTGA+TGG | + | chr3.4:97834527-97834546 | None:intergenic | 20.0% |
| !! | ATTTGATGAAGAATTAATGA+TGG | - | chr3.4:97835666-97835685 | MS.gene064754:intron | 20.0% |
| !! | CATTATAATATGTTTGTAAG+TGG | - | chr3.4:97834673-97834692 | MS.gene064754:CDS | 20.0% |
| !! | TACTCAAATTCTTAATCATT+TGG | - | chr3.4:97835109-97835128 | MS.gene064754:intron | 20.0% |
| !! | TCTAATCTATAGATTCATAA+TGG | - | chr3.4:97835373-97835392 | MS.gene064754:intron | 20.0% |
| !! | TTAGAGAATAGAGATTAATA+AGG | - | chr3.4:97835250-97835269 | MS.gene064754:intron | 20.0% |
| !! | TTATAATATGTTTGTAAGTG+GGG | - | chr3.4:97834675-97834694 | MS.gene064754:CDS | 20.0% |
| !! | TTTAAATCACCAATTAAACT+TGG | - | chr3.4:97834583-97834602 | MS.gene064754:CDS | 20.0% |
| !!! | ATCATCTAATGGATAAAATT+TGG | + | chr3.4:97834749-97834768 | None:intergenic | 20.0% |
| !!! | TAAGATGCACAAAATAATTT+TGG | + | chr3.4:97835233-97835252 | None:intergenic | 20.0% |
| !!! | TTCAATATAGTCTTACAATA+TGG | - | chr3.4:97835066-97835085 | MS.gene064754:intron | 20.0% |
| !!! | TTTTACTTTTTACATACCTA+CGG | + | chr3.4:97835155-97835174 | None:intergenic | 20.0% |
| ! | AAGTCATTAATTCCACTTAT+CGG | - | chr3.4:97835814-97835833 | MS.gene064754:CDS | 25.0% |
| ! | AATCTATAGATTAGAAGACA+TGG | + | chr3.4:97835368-97835387 | None:intergenic | 25.0% |
| ! | ACTTCCTTTATTCAATTAGA+CGG | + | chr3.4:97834966-97834985 | None:intergenic | 25.0% |
| ! | GTAACTCAATCTTACAAAAT+CGG | + | chr3.4:97834719-97834738 | None:intergenic | 25.0% |
| ! | GTTTCAAAATAACATAGGAT+AGG | + | chr3.4:97834858-97834877 | None:intergenic | 25.0% |
| ! | TTAAAAAAATATTGGCCAAG+AGG | - | chr3.4:97834787-97834806 | MS.gene064754:CDS | 25.0% |
| ! | TTACTGTGTAAGATTAGTAT+CGG | - | chr3.4:97835183-97835202 | MS.gene064754:intron | 25.0% |
| !! | ATAATGGAGCAAATGAAAAT+GGG | - | chr3.4:97835389-97835408 | MS.gene064754:intron | 25.0% |
| !! | ATTAGAGTATCAAAATGGTT+AGG | - | chr3.4:97834544-97834563 | MS.gene064754:CDS | 25.0% |
| !! | TAAAGAAGAAAGCATGTTAA+TGG | - | chr3.4:97835483-97835502 | MS.gene064754:intron | 25.0% |
| !!! | AGGTATTTTTTCTAGGAAAA+AGG | - | chr3.4:97835837-97835856 | MS.gene064754:CDS | 25.0% |
| !!! | ATCCTATGTTATTTTGAAAC+AGG | - | chr3.4:97834858-97834877 | MS.gene064754:intron | 25.0% |
| !!! | GGTATTTTTTCTAGGAAAAA+GGG | - | chr3.4:97835838-97835857 | MS.gene064754:CDS | 25.0% |
| !!! | TCCTATGTTATTTTGAAACA+GGG | - | chr3.4:97834859-97834878 | MS.gene064754:intron | 25.0% |
| AAAGAGGCTGTTACATTATT+CGG | - | chr3.4:97834436-97834455 | MS.gene064754:CDS | 30.0% | |
| AAATACAGAGATAGACACAA+GGG | - | chr3.4:97834396-97834415 | MS.gene064754:CDS | 30.0% | |
| ACCCTGTTTCAAAATAACAT+AGG | + | chr3.4:97834863-97834882 | None:intergenic | 30.0% | |
| CGAAACTGAAAAAAATCAAG+TGG | - | chr3.4:97835576-97835595 | MS.gene064754:intron | 30.0% | |
| TAATGGAGCAAATGAAAATG+GGG | - | chr3.4:97835390-97835409 | MS.gene064754:intron | 30.0% | |
| TGATGGAGTTTCAAAAGAAA+AGG | - | chr3.4:97835683-97835702 | MS.gene064754:intron | 30.0% | |
| TTCACCGTCTAATTGAATAA+AGG | - | chr3.4:97834959-97834978 | MS.gene064754:intron | 30.0% | |
| ! | CATAATGGAGCAAATGAAAA+TGG | - | chr3.4:97835388-97835407 | MS.gene064754:intron | 30.0% |
| ! | TCTTGAACTTTTCACTTCAA+AGG | + | chr3.4:97835647-97835666 | None:intergenic | 30.0% |
| !! | TCTTACAAAATCGGTTTGTA+AGG | + | chr3.4:97834710-97834729 | None:intergenic | 30.0% |
| !!! | TTATCGGAGGTATTTTTTCT+AGG | - | chr3.4:97835830-97835849 | MS.gene064754:CDS | 30.0% |
| AAATGTCCAAGAATTCCTCT+TGG | + | chr3.4:97834805-97834824 | None:intergenic | 35.0% | |
| AACATGACATGAGAAATGGA+CGG | + | chr3.4:97835730-97835749 | None:intergenic | 35.0% | |
| AGGAGAATCTTATTGATGGA+TGG | + | chr3.4:97834838-97834857 | None:intergenic | 35.0% | |
| AGGTAACATGACATGAGAAA+TGG | + | chr3.4:97835734-97835753 | None:intergenic | 35.0% | |
| GAAAAAATACCTCCGATAAG+TGG | + | chr3.4:97835829-97835848 | None:intergenic | 35.0% | |
| GAAATACAGAGATAGACACA+AGG | - | chr3.4:97834395-97834414 | MS.gene064754:CDS | 35.0% | |
| GGATAGGAGAATCTTATTGA+TGG | + | chr3.4:97834842-97834861 | None:intergenic | 35.0% | |
| GTGACACATCCAAGTTTAAT+TGG | + | chr3.4:97834595-97834614 | None:intergenic | 35.0% | |
| TAATGTAACAGCCTCTTTGA+CGG | + | chr3.4:97834434-97834453 | None:intergenic | 35.0% | |
| TCATTAATTCCACTTATCGG+AGG | - | chr3.4:97835817-97835836 | MS.gene064754:CDS | 35.0% | |
| TCCTCATCATCACTTGTTTA+TGG | - | chr3.4:97835292-97835311 | MS.gene064754:intron | 35.0% | |
| TCTTCTTCTTCCTAAGAGAA+GGG | + | chr3.4:97835792-97835811 | None:intergenic | 35.0% | |
| TGTGTCTATCTCTGTATTTC+TGG | + | chr3.4:97834395-97834414 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCCTAAGAGA+AGG | + | chr3.4:97835793-97835812 | None:intergenic | 35.0% | |
| ! | ACCATAAACAAGTGATGATG+AGG | + | chr3.4:97835296-97835315 | None:intergenic | 35.0% |
| !! | TTCGGTGATAAAGTTTTAGC+TGG | - | chr3.4:97834454-97834473 | MS.gene064754:CDS | 35.0% |
| ACATTGTATGTCATCTCTCC+AGG | - | chr3.4:97835507-97835526 | MS.gene064754:intron | 40.0% | |
| ATGTTACCTCAAGGTCATCA+AGG | - | chr3.4:97835745-97835764 | MS.gene064754:CDS | 40.0% | |
| TATTGGCCAAGAGGAATTCT+TGG | - | chr3.4:97834796-97834815 | MS.gene064754:CDS | 40.0% | |
| TCTCATGTCATGTTACCTCA+AGG | - | chr3.4:97835736-97835755 | MS.gene064754:CDS | 40.0% | |
| TGCAACTAAACTCAAACACC+AGG | - | chr3.4:97834492-97834511 | MS.gene064754:CDS | 40.0% | |
| TGTCGTATATCATAGAACGC+AGG | + | chr3.4:97834655-97834674 | None:intergenic | 40.0% | |
| ! | ATGTGATGGTGTATAGAACC+TGG | + | chr3.4:97834513-97834532 | None:intergenic | 40.0% |
| !! | AAGGTGTTGAGAAGTTGGAA+AGG | - | chr3.4:97835764-97835783 | MS.gene064754:CDS | 40.0% |
| !! | TCATCAAGGTGTTGAGAAGT+TGG | - | chr3.4:97835759-97835778 | MS.gene064754:CDS | 40.0% |
| ATGACATGAGAAATGGACGG+TGG | + | chr3.4:97835727-97835746 | None:intergenic | 45.0% | |
| TGAAAACGTTACAGCTGAGC+TGG | - | chr3.4:97835432-97835451 | MS.gene064754:intron | 45.0% | |
| ! | ATGGGGTTGATCAGTCTTCA+AGG | - | chr3.4:97835407-97835426 | MS.gene064754:intron | 45.0% |
| ! | TCAACACCTTGATGACCTTG+AGG | + | chr3.4:97835754-97835773 | None:intergenic | 45.0% |
| ! | TGGGGTTGATCAGTCTTCAA+GGG | - | chr3.4:97835408-97835427 | MS.gene064754:intron | 45.0% |
| !! | CAAAATCGGTTTGTAAGGCG+AGG | + | chr3.4:97834705-97834724 | None:intergenic | 45.0% |
| !! | TATACTTATTAAAAAAATAT+TGG | - | chr3.4:97834779-97834798 | MS.gene064754:CDS | 5.0% |
| AAACGTTACAGCTGAGCTGG+AGG | - | chr3.4:97835435-97835454 | MS.gene064754:intron | 50.0% | |
| !! | TGGAAAGGCACCCTTCTCTT+AGG | - | chr3.4:97835779-97835798 | MS.gene064754:CDS | 50.0% |
| GCCATTCAGGTCCGTCAAAG+AGG | - | chr3.4:97834420-97834439 | MS.gene064754:CDS | 55.0% | |
| ! | GCCTCTTTGACGGACCTGAA+TGG | + | chr3.4:97834424-97834443 | None:intergenic | 55.0% |
| ! | TCGTGCGTTCGAGTTCTTCC+TGG | + | chr3.4:97835528-97835547 | None:intergenic | 55.0% |
| !! | TAGACACAAGGGCGCCATTC+AGG | - | chr3.4:97834407-97834426 | MS.gene064754:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97834358 | 97835882 | 97834358 | ID=MS.gene064754 |
| chr3.4 | mRNA | 97834358 | 97835882 | 97834358 | ID=MS.gene064754.t1;Parent=MS.gene064754 |
| chr3.4 | exon | 97835727 | 97835882 | 97835727 | ID=MS.gene064754.t1.exon1;Parent=MS.gene064754.t1 |
| chr3.4 | CDS | 97835727 | 97835882 | 97835727 | ID=cds.MS.gene064754.t1;Parent=MS.gene064754.t1 |
| chr3.4 | exon | 97834358 | 97834855 | 97834358 | ID=MS.gene064754.t1.exon2;Parent=MS.gene064754.t1 |
| chr3.4 | CDS | 97834358 | 97834855 | 97834358 | ID=cds.MS.gene064754.t1;Parent=MS.gene064754.t1 |
| Gene Sequence |
| Protein sequence |
