Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055371.t1 | XP_013443805.1 | 95.2 | 168 | 8 | 0 | 1 | 168 | 1 | 168 | 9.30E-80 | 306.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055371.t1 | I3SHZ2 | 95.2 | 168 | 8 | 0 | 1 | 168 | 1 | 168 | 6.7e-80 | 306.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055371 | MS.gene058909 | 0.835445 | 1.67E-56 | -1.69E-46 |
| MS.gene055371 | MS.gene058912 | 0.834057 | 3.73E-56 | -1.69E-46 |
| MS.gene055371 | MS.gene064754 | 0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene055371 | MS.gene34943 | 0.806188 | 9.29E-50 | -1.69E-46 |
| MS.gene055371 | MS.gene47958 | 0.835172 | 1.96E-56 | -1.69E-46 |
| MS.gene055371 | MS.gene47960 | 0.832858 | 7.44E-56 | -1.69E-46 |
| MS.gene055371 | MS.gene47963 | 0.839995 | 1.13E-57 | -1.69E-46 |
| MS.gene055371 | MS.gene62780 | 0.809453 | 1.88E-50 | -1.69E-46 |
| MS.gene055371 | MS.gene69404 | 0.812749 | 3.62E-51 | -1.69E-46 |
| MS.gene055371 | MS.gene70711 | 0.819847 | 9.37E-53 | -1.69E-46 |
| MS.gene055371 | MS.gene75180 | 0.810168 | 1.32E-50 | -1.69E-46 |
| MS.gene055371 | MS.gene91794 | 0.949682 | 7.76E-108 | -1.69E-46 |
| MS.gene055371 | MS.gene96235 | 0.839034 | 2.02E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055371.t1 | MTR_8g006765 | 95.238 | 168 | 8 | 0 | 1 | 168 | 1 | 168 | 9.37e-116 | 325 |
| MS.gene055371.t1 | MTR_4g094628 | 59.627 | 161 | 62 | 3 | 1 | 160 | 1 | 159 | 6.44e-62 | 191 |
| MS.gene055371.t1 | MTR_7g407110 | 50.704 | 71 | 33 | 2 | 1 | 71 | 148 | 216 | 1.39e-14 | 70.1 |
| MS.gene055371.t1 | MTR_8g021220 | 60.870 | 46 | 18 | 0 | 82 | 127 | 33 | 78 | 2.65e-13 | 62.4 |
| MS.gene055371.t1 | MTR_8g009870 | 28.333 | 120 | 83 | 2 | 32 | 148 | 33 | 152 | 4.25e-12 | 61.2 |
| MS.gene055371.t1 | MTR_8g009870 | 28.333 | 120 | 83 | 2 | 32 | 148 | 29 | 148 | 4.67e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055371.t1 | AT4G37445 | 52.830 | 159 | 74 | 1 | 6 | 163 | 3 | 161 | 1.84e-55 | 172 |
| MS.gene055371.t1 | AT4G37445 | 54.167 | 144 | 66 | 0 | 20 | 163 | 2 | 145 | 1.15e-52 | 164 |
Find 32 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAAAGTGAAAATTAAAT+AGG | 0.234371 | 8.1:-87019749 | None:intergenic |
| CATTGATTTCATGATCAATA+AGG | 0.282087 | 8.1:+87019520 | MS.gene055371:CDS |
| TTTCTTCAATGACATTCTTT+TGG | 0.307917 | 8.1:-87019720 | None:intergenic |
| ACCATCACACAAGGAAATTA+AGG | 0.323749 | 8.1:+87019442 | MS.gene055371:CDS |
| GATGATTCTATGATGATAAC+TGG | 0.356747 | 8.1:+87019560 | MS.gene055371:CDS |
| CAGACATTAGATCTTGTATC+AGG | 0.382635 | 8.1:+87018857 | MS.gene055371:CDS |
| CTGATACAAGATCTAATGTC+TGG | 0.398337 | 8.1:-87018856 | None:intergenic |
| ATGAGTTACATGGGAGGATC+AGG | 0.435033 | 8.1:+87018682 | MS.gene055371:CDS |
| TATGATGATAACTGGAATAG+TGG | 0.452300 | 8.1:+87019568 | MS.gene055371:CDS |
| GCTATGGTAGCTAAGAGAAC+AGG | 0.456395 | 8.1:+87019602 | MS.gene055371:CDS |
| CTCTTAGCTACCATAGCTGC+TGG | 0.471596 | 8.1:-87019596 | None:intergenic |
| ATGTTTCTTCTTATCTTCCT+CGG | 0.474190 | 8.1:-87019492 | None:intergenic |
| ACTATTAACATGGCCTTGCC+TGG | 0.488789 | 8.1:+87019404 | MS.gene055371:CDS |
| TCCTTAATTTCCTTGTGTGA+TGG | 0.508644 | 8.1:-87019443 | None:intergenic |
| ATCATAATGCTTACCAGGCA+AGG | 0.514482 | 8.1:-87019417 | None:intergenic |
| GAGCTAAAATAGTAGCTGTT+GGG | 0.514762 | 8.1:-87019679 | None:intergenic |
| GTTGGGACAAAGATAACATC+AGG | 0.516476 | 8.1:-87019662 | None:intergenic |
| ATCAAGAATGGCAGTGTGGA+AGG | 0.518421 | 8.1:-87018927 | None:intergenic |
| AGAGCTAAAATAGTAGCTGT+TGG | 0.518631 | 8.1:-87019680 | None:intergenic |
| AGTGGCACCTCCAGCAGCTA+TGG | 0.520301 | 8.1:+87019586 | MS.gene055371:CDS |
| TTACTTGAAGATATCAAGAA+TGG | 0.537162 | 8.1:-87018939 | None:intergenic |
| GGGGATGATCATGAGTTACA+TGG | 0.552167 | 8.1:+87018672 | MS.gene055371:CDS |
| GGTACATCATAATGCTTACC+AGG | 0.555755 | 8.1:-87019422 | None:intergenic |
| GACAAAGATAACATCAGGAA+TGG | 0.591291 | 8.1:-87019657 | None:intergenic |
| TGATACAAGATCTAATGTCT+GGG | 0.602501 | 8.1:-87018855 | None:intergenic |
| TTAGCTACCATAGCTGCTGG+AGG | 0.629958 | 8.1:-87019593 | None:intergenic |
| GCTTTCATAACACTTAGTTG+AGG | 0.635041 | 8.1:-87019635 | None:intergenic |
| GGGATGATCATGAGTTACAT+GGG | 0.647259 | 8.1:+87018673 | MS.gene055371:CDS |
| AAACTGGAATAAAACATCCG+AGG | 0.684088 | 8.1:+87019475 | MS.gene055371:CDS |
| TTATGATGTACCATCACACA+AGG | 0.699802 | 8.1:+87019433 | MS.gene055371:CDS |
| AGATATCAAGAATGGCAGTG+TGG | 0.711163 | 8.1:-87018931 | None:intergenic |
| ATGATCATGAGTTACATGGG+AGG | 0.727517 | 8.1:+87018676 | MS.gene055371:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTGTAAAAAAAAAAAAAA+AGG | + | chr8.1:87018995-87019014 | MS.gene055371:intron | 10.0% |
| !! | AAAAAAAAAAAAAAGGAAAG+TGG | + | chr8.1:87019002-87019021 | MS.gene055371:intron | 15.0% |
| !! | AAAAAAAAAAAAAGGAAAGT+GGG | + | chr8.1:87019003-87019022 | MS.gene055371:intron | 15.0% |
| !! | AAAATTTAACATATTTCATC+AGG | + | chr8.1:87018818-87018837 | MS.gene055371:intron | 15.0% |
| !!! | TCAAATAATCATCTAAAATA+TGG | + | chr8.1:87019118-87019137 | MS.gene055371:intron | 15.0% |
| !! | AAAAAAAAAAAAGGAAAGTG+GGG | + | chr8.1:87019004-87019023 | MS.gene055371:intron | 20.0% |
| !! | AAAAAAACTAGTGGAATTAA+AGG | - | chr8.1:87018730-87018749 | None:intergenic | 20.0% |
| !! | AGCAATAAAAAAAAAACTAG+TGG | - | chr8.1:87018739-87018758 | None:intergenic | 20.0% |
| !! | CAAACTATATACTAGTATAA+TGG | + | chr8.1:87019059-87019078 | MS.gene055371:intron | 20.0% |
| !! | TTCAACATTTGATATCAATT+TGG | + | chr8.1:87019341-87019360 | MS.gene055371:intron | 20.0% |
| !! | TTGATATCAAATGTTGAATA+TGG | - | chr8.1:87019339-87019358 | None:intergenic | 20.0% |
| !!! | CTAGTTTTTTTTTTATTGCT+AGG | + | chr8.1:87018739-87018758 | MS.gene055371:intron | 20.0% |
| ! | ACCTTAGTTGCATAAATTTA+AGG | - | chr8.1:87019279-87019298 | None:intergenic | 25.0% |
| ! | ATATGGTCATGGTAAAATTA+AGG | + | chr8.1:87019135-87019154 | MS.gene055371:intron | 25.0% |
| ! | CCTTAGTTGCATAAATTTAA+GGG | - | chr8.1:87019278-87019297 | None:intergenic | 25.0% |
| ! | TTACTTGAAGATATCAAGAA+TGG | - | chr8.1:87018942-87018961 | None:intergenic | 25.0% |
| !! | AATCATCTAAAATATGGTCA+TGG | + | chr8.1:87019124-87019143 | MS.gene055371:intron | 25.0% |
| !! | GATTATCATTTTGTCAAAAG+TGG | - | chr8.1:87019227-87019246 | None:intergenic | 25.0% |
| !! | TTTCTTCAATGACATTCTTT+TGG | - | chr8.1:87019723-87019742 | None:intergenic | 25.0% |
| !! | TTTTACTAGCACTATTAACA+TGG | + | chr8.1:87019394-87019413 | MS.gene055371:intron | 25.0% |
| !!! | CATTGATTTCATGATCAATA+AGG | + | chr8.1:87019520-87019539 | MS.gene055371:CDS | 25.0% |
| ATGTTTCTTCTTATCTTCCT+CGG | - | chr8.1:87019495-87019514 | None:intergenic | 30.0% | |
| CCCTTAAATTTATGCAACTA+AGG | + | chr8.1:87019275-87019294 | MS.gene055371:intron | 30.0% | |
| GATGATTCTATGATGATAAC+TGG | + | chr8.1:87019560-87019579 | MS.gene055371:CDS | 30.0% | |
| TAACATATTTCATCAGGTGA+GGG | + | chr8.1:87018824-87018843 | MS.gene055371:intron | 30.0% | |
| TATGATGATAACTGGAATAG+TGG | + | chr8.1:87019568-87019587 | MS.gene055371:CDS | 30.0% | |
| TGATACAAGATCTAATGTCT+GGG | - | chr8.1:87018858-87018877 | None:intergenic | 30.0% | |
| TTAACATATTTCATCAGGTG+AGG | + | chr8.1:87018823-87018842 | MS.gene055371:intron | 30.0% | |
| ! | TTAAGGAAGTTTTCGAAAAC+TGG | + | chr8.1:87019459-87019478 | MS.gene055371:CDS | 30.0% |
| AAACTGGAATAAAACATCCG+AGG | + | chr8.1:87019475-87019494 | MS.gene055371:CDS | 35.0% | |
| ACCATCACACAAGGAAATTA+AGG | + | chr8.1:87019442-87019461 | MS.gene055371:CDS | 35.0% | |
| AGAGCTAAAATAGTAGCTGT+TGG | - | chr8.1:87019683-87019702 | None:intergenic | 35.0% | |
| CAGACATTAGATCTTGTATC+AGG | + | chr8.1:87018857-87018876 | MS.gene055371:CDS | 35.0% | |
| CTGATACAAGATCTAATGTC+TGG | - | chr8.1:87018859-87018878 | None:intergenic | 35.0% | |
| GACAAAGATAACATCAGGAA+TGG | - | chr8.1:87019660-87019679 | None:intergenic | 35.0% | |
| GAGCTAAAATAGTAGCTGTT+GGG | - | chr8.1:87019682-87019701 | None:intergenic | 35.0% | |
| GCTTTCATAACACTTAGTTG+AGG | - | chr8.1:87019638-87019657 | None:intergenic | 35.0% | |
| TACATACCATGCATGACAAA+AGG | - | chr8.1:87019200-87019219 | None:intergenic | 35.0% | |
| TCCTTAATTTCCTTGTGTGA+TGG | - | chr8.1:87019446-87019465 | None:intergenic | 35.0% | |
| TTATGATGTACCATCACACA+AGG | + | chr8.1:87019433-87019452 | MS.gene055371:CDS | 35.0% | |
| ACCATGCATGACAAAAGGTT+TGG | - | chr8.1:87019195-87019214 | None:intergenic | 40.0% | |
| AGATATCAAGAATGGCAGTG+TGG | - | chr8.1:87018934-87018953 | None:intergenic | 40.0% | |
| ATCATAATGCTTACCAGGCA+AGG | - | chr8.1:87019420-87019439 | None:intergenic | 40.0% | |
| ATGATCATGAGTTACATGGG+AGG | + | chr8.1:87018676-87018695 | MS.gene055371:CDS | 40.0% | |
| GGGATGATCATGAGTTACAT+GGG | + | chr8.1:87018673-87018692 | MS.gene055371:CDS | 40.0% | |
| GGTACATCATAATGCTTACC+AGG | - | chr8.1:87019425-87019444 | None:intergenic | 40.0% | |
| GTTGGGACAAAGATAACATC+AGG | - | chr8.1:87019665-87019684 | None:intergenic | 40.0% | |
| ! | ACCAAACCTTTTGTCATGCA+TGG | + | chr8.1:87019191-87019210 | MS.gene055371:intron | 40.0% |
| ATCAAGAATGGCAGTGTGGA+AGG | - | chr8.1:87018930-87018949 | None:intergenic | 45.0% | |
| ATGAGTTACATGGGAGGATC+AGG | + | chr8.1:87018682-87018701 | MS.gene055371:CDS | 45.0% | |
| GCTATGGTAGCTAAGAGAAC+AGG | + | chr8.1:87019602-87019621 | MS.gene055371:CDS | 45.0% | |
| GGGGATGATCATGAGTTACA+TGG | + | chr8.1:87018672-87018691 | MS.gene055371:CDS | 45.0% | |
| ! | ACTATTAACATGGCCTTGCC+TGG | + | chr8.1:87019404-87019423 | MS.gene055371:CDS | 45.0% |
| !!! | AAATATGAATATTATTTTAA+AGG | + | chr8.1:87019085-87019104 | MS.gene055371:intron | 5.0% |
| CTCTTAGCTACCATAGCTGC+TGG | - | chr8.1:87019599-87019618 | None:intergenic | 50.0% | |
| TTAGCTACCATAGCTGCTGG+AGG | - | chr8.1:87019596-87019615 | None:intergenic | 50.0% | |
| AGTGGCACCTCCAGCAGCTA+TGG | + | chr8.1:87019586-87019605 | MS.gene055371:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 87018670 | 87019757 | 87018670 | ID=MS.gene055371 |
| chr8.1 | mRNA | 87018670 | 87019757 | 87018670 | ID=MS.gene055371.t1;Parent=MS.gene055371 |
| chr8.1 | exon | 87018670 | 87018703 | 87018670 | ID=MS.gene055371.t1.exon1;Parent=MS.gene055371.t1 |
| chr8.1 | CDS | 87018670 | 87018703 | 87018670 | ID=cds.MS.gene055371.t1;Parent=MS.gene055371.t1 |
| chr8.1 | exon | 87018840 | 87018957 | 87018840 | ID=MS.gene055371.t1.exon2;Parent=MS.gene055371.t1 |
| chr8.1 | CDS | 87018840 | 87018957 | 87018840 | ID=cds.MS.gene055371.t1;Parent=MS.gene055371.t1 |
| chr8.1 | exon | 87019403 | 87019757 | 87019403 | ID=MS.gene055371.t1.exon3;Parent=MS.gene055371.t1 |
| chr8.1 | CDS | 87019403 | 87019757 | 87019403 | ID=cds.MS.gene055371.t1;Parent=MS.gene055371.t1 |
| Gene Sequence |
| Protein sequence |
