Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064758.t1 | PNX75878.1 | 74.4 | 90 | 23 | 0 | 1 | 90 | 1 | 90 | 7.30E-23 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064758.t1 | A0A2K3LBG3 | 74.4 | 90 | 23 | 0 | 1 | 90 | 1 | 90 | 5.2e-23 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050502 | MS.gene064758 | 0.800963 | 1.13E-48 | -1.69E-46 |
| MS.gene052999 | MS.gene064758 | 0.814307 | 1.65E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene064758 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene064758 | 0.813835 | 2.09E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene064758 | 0.817124 | 3.88E-52 | -1.69E-46 |
| MS.gene057019 | MS.gene064758 | 0.805644 | 1.21E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene064758 | 0.82942 | 5.20E-55 | -1.69E-46 |
| MS.gene060372 | MS.gene064758 | 0.820956 | 5.21E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 3.4:+97873546 | None:intergenic |
| TCTCCATCTTGTTCATGCTT+TGG | 0.293177 | 3.4:-97874309 | MS.gene064758:CDS |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 3.4:+97873532 | None:intergenic |
| TGCGTGCCACCGGGAACTTC+TGG | 0.308001 | 3.4:-97873572 | MS.gene064758:CDS |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 3.4:+97873510 | None:intergenic |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 3.4:-97873625 | MS.gene064758:CDS |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 3.4:+97873519 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 3.4:+97873523 | None:intergenic |
| TCGGTGCAACTGCGTGCCAC+CGG | 0.451569 | 3.4:-97873582 | MS.gene064758:CDS |
| GATGAATTCAACTCAATTCA+AGG | 0.452504 | 3.4:+97873488 | None:intergenic |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 3.4:+97873615 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 3.4:+97873636 | None:intergenic |
| TCTTGGTTACCAGAAGTTCC+CGG | 0.467040 | 3.4:+97873563 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 3.4:-97873617 | MS.gene064758:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 3.4:+97873511 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 3.4:+97873514 | None:intergenic |
| AGCATGAACAAGATGGAGAA+TGG | 0.540714 | 3.4:+97874313 | None:intergenic |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 3.4:+97873533 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+GGG | 0.551251 | 3.4:-97873581 | MS.gene064758:CDS |
| TGTTCATGCTTTGGACCAGC+CGG | 0.555470 | 3.4:-97874300 | MS.gene064758:intron |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 3.4:-97873661 | MS.gene064758:CDS |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 3.4:-97873677 | MS.gene064758:intron |
| GGTCCAAAGCATGAACAAGA+TGG | 0.576917 | 3.4:+97874306 | None:intergenic |
| ATGAATTCAACTCAATTCAA+GGG | 0.589084 | 3.4:+97873489 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 3.4:+97873620 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 3.4:-97873601 | MS.gene064758:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 3.4:-97873624 | MS.gene064758:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 3.4:-97873643 | MS.gene064758:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 3.4:-97873527 | MS.gene064758:CDS |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 3.4:-97873524 | MS.gene064758:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 3.4:+97873609 | None:intergenic |
| TGGTTACCAGAAGTTCCCGG+TGG | 0.747138 | 3.4:+97873566 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATATGAACTAAACTA+AGG | - | chr3.4:97873837-97873856 | MS.gene064758:intron | 15.0% |
| !! | TGGAAAAAATATATAAAAAC+TGG | - | chr3.4:97873791-97873810 | MS.gene064758:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | chr3.4:97873771-97873790 | MS.gene064758:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | chr3.4:97874177-97874196 | MS.gene064758:intron | 20.0% |
| !! | GGATAAAAGATTAGTAAAAA+AGG | - | chr3.4:97873950-97873969 | MS.gene064758:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | chr3.4:97873629-97873648 | None:intergenic | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | chr3.4:97873929-97873948 | MS.gene064758:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | chr3.4:97873928-97873947 | MS.gene064758:intron | 25.0% |
| !! | TTAATTACATGTGTTTTCGT+AGG | - | chr3.4:97873665-97873684 | MS.gene064758:CDS | 25.0% |
| ! | CTACTTCACACACATATTTT+TGG | + | chr3.4:97874078-97874097 | None:intergenic | 30.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | chr3.4:97873576-97873595 | None:intergenic | 30.0% |
| AGCATGAACAAGATGGAGAA+TGG | + | chr3.4:97873544-97873563 | None:intergenic | 40.0% | |
| TAAAGAAAACGCTTGGAGCT+TGG | + | chr3.4:97873622-97873641 | None:intergenic | 40.0% | |
| TCTCCATCTTGTTCATGCTT+TGG | - | chr3.4:97873545-97873564 | MS.gene064758:CDS | 40.0% | |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | chr3.4:97873714-97873733 | MS.gene064758:intron | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | chr3.4:97873572-97873591 | None:intergenic | 40.0% |
| AACCAGACAGAAGCAGTTGA+TGG | + | chr3.4:97873753-97873772 | None:intergenic | 45.0% | |
| CATAGCAGGGACACATTTCT+TGG | + | chr3.4:97874311-97874330 | None:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | chr3.4:97873551-97873570 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+CGG | + | chr3.4:97874294-97874313 | None:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | chr3.4:97873693-97873712 | None:intergenic | 45.0% | |
| TTCCATCAACTGCTTCTGTC+TGG | - | chr3.4:97873748-97873767 | MS.gene064758:intron | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr3.4:97874211-97874230 | MS.gene064758:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | chr3.4:97873653-97873672 | None:intergenic | 5.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr3.4:97874327-97874346 | MS.gene064758:CDS | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | chr3.4:97873554-97873573 | MS.gene064758:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr3.4:97874253-97874272 | MS.gene064758:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr3.4:97874324-97874343 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr3.4:97874248-97874267 | None:intergenic | 55.0% | |
| TGGTTACCAGAAGTTCCCGG+TGG | + | chr3.4:97874291-97874310 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr3.4:97874193-97874212 | MS.gene064758:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr3.4:97874325-97874344 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr3.4:97874346-97874365 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr3.4:97874330-97874349 | MS.gene064758:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr3.4:97874221-97874240 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr3.4:97874338-97874357 | None:intergenic | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr3.4:97874242-97874261 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr3.4:97874334-97874353 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr3.4:97874343-97874362 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr3.4:97874347-97874366 | None:intergenic | 65.0% | |
| TCGGTGCAACTGCGTGCCAC+CGG | - | chr3.4:97874272-97874291 | MS.gene064758:intron | 65.0% | |
| TGCGTGCCACCGGGAACTTC+TGG | - | chr3.4:97874282-97874301 | MS.gene064758:intron | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr3.4:97874237-97874256 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr3.4:97874237-97874256 | MS.gene064758:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+GGG | - | chr3.4:97874273-97874292 | MS.gene064758:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr3.4:97874230-97874249 | MS.gene064758:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr3.4:97874229-97874248 | MS.gene064758:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97873495 | 97874381 | 97873495 | ID=MS.gene064758 |
| chr3.4 | mRNA | 97873495 | 97874381 | 97873495 | ID=MS.gene064758.t1;Parent=MS.gene064758 |
| chr3.4 | exon | 97874301 | 97874381 | 97874301 | ID=MS.gene064758.t1.exon1;Parent=MS.gene064758.t1 |
| chr3.4 | CDS | 97874301 | 97874381 | 97874301 | ID=cds.MS.gene064758.t1;Parent=MS.gene064758.t1 |
| chr3.4 | exon | 97873495 | 97873686 | 97873495 | ID=MS.gene064758.t1.exon2;Parent=MS.gene064758.t1 |
| chr3.4 | CDS | 97873495 | 97873686 | 97873495 | ID=cds.MS.gene064758.t1;Parent=MS.gene064758.t1 |
| Gene Sequence |
| Protein sequence |
