Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064796.t1 | XP_003603835.1 | 92.7 | 247 | 7 | 3 | 1 | 238 | 1 | 245 | 6.50E-111 | 410.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064796.t1 | Q9SGS5 | 39.5 | 276 | 107 | 10 | 6 | 237 | 13 | 272 | 9.7e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064796.t1 | G7J5W8 | 92.7 | 247 | 7 | 3 | 1 | 238 | 1 | 245 | 4.7e-111 | 410.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene064796 | 0.833896 | 4.10E-56 | -1.69E-46 |
| MS.gene053826 | MS.gene064796 | 0.812936 | 3.30E-51 | -1.69E-46 |
| MS.gene055550 | MS.gene064796 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene056311 | MS.gene064796 | 0.814081 | 1.85E-51 | -1.69E-46 |
| MS.gene058493 | MS.gene064796 | 0.80008 | 1.70E-48 | -1.69E-46 |
| MS.gene058494 | MS.gene064796 | 0.810566 | 1.08E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064796.t1 | MTR_3g114750 | 91.903 | 247 | 9 | 3 | 1 | 238 | 1 | 245 | 4.06e-149 | 415 |
| MS.gene064796.t1 | MTR_1g026110 | 49.225 | 258 | 91 | 9 | 2 | 237 | 4 | 243 | 1.02e-63 | 199 |
| MS.gene064796.t1 | MTR_5g023030 | 33.951 | 162 | 88 | 4 | 1 | 158 | 1 | 147 | 1.12e-12 | 63.9 |
| MS.gene064796.t1 | MTR_5g023030 | 33.951 | 162 | 88 | 4 | 1 | 158 | 1 | 147 | 4.28e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064796.t1 | AT1G76070 | 34.815 | 270 | 112 | 12 | 14 | 237 | 21 | 272 | 9.24e-30 | 112 |
| MS.gene064796.t1 | AT1G20310 | 28.897 | 263 | 133 | 10 | 1 | 234 | 1 | 238 | 1.86e-19 | 84.3 |
Find 47 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTCTTCCCTCAGATTT+TGG | 0.122137 | 3.4:-98246755 | None:intergenic |
| ATCAATTCCATTAATATCTT+TGG | 0.179497 | 3.4:-98246594 | None:intergenic |
| CTTGGTGGTGGTGGTGTTGC+TGG | 0.256841 | 3.4:+98247007 | MS.gene064796:CDS |
| CCAAGTTTGATCTAATTTAC+TGG | 0.265351 | 3.4:-98247121 | None:intergenic |
| AGAAAGTCCAAAGATATTAA+TGG | 0.282367 | 3.4:+98246587 | MS.gene064796:CDS |
| AGATCTTGTTCCTTAGCTTA+AGG | 0.361689 | 3.4:-98246427 | None:intergenic |
| GGTGTTGCTGGTTGTAGTCT+TGG | 0.383396 | 3.4:+98247019 | MS.gene064796:CDS |
| AAAAGATGTTATTTCATGTC+GGG | 0.387650 | 3.4:+98246729 | MS.gene064796:CDS |
| ATCCCTTTCTCTGCACCTCT+TGG | 0.403077 | 3.4:+98246989 | MS.gene064796:CDS |
| TCTAGCTTCATGTGGAATCA+TGG | 0.405796 | 3.4:-98246564 | None:intergenic |
| TCCACCTAGGCCTCTACAAC+TGG | 0.412969 | 3.4:+98247096 | MS.gene064796:CDS |
| CCTTTCTCTGCACCTCTTGG+TGG | 0.430613 | 3.4:+98246992 | MS.gene064796:CDS |
| TCTGCACCTCTTGGTGGTGG+TGG | 0.444151 | 3.4:+98246998 | MS.gene064796:CDS |
| CTTGATTTGTGATCCCTACC+TGG | 0.448791 | 3.4:-98246503 | None:intergenic |
| GACTTTCTTCTAGCTTCATG+TGG | 0.470996 | 3.4:-98246572 | None:intergenic |
| GGATCCAGTTGTAGAGGCCT+AGG | 0.476355 | 3.4:-98247100 | None:intergenic |
| TTCTCTGCACCTCTTGGTGG+TGG | 0.477822 | 3.4:+98246995 | MS.gene064796:CDS |
| TAGTAATTATCTATTTCCTC+TGG | 0.477991 | 3.4:-98246908 | None:intergenic |
| CACGTAAAGAAATTAATCTA+TGG | 0.479566 | 3.4:+98247056 | MS.gene064796:CDS |
| TGTGGAATCATGGATGCAAT+TGG | 0.480921 | 3.4:-98246554 | None:intergenic |
| CCACCAAGAGGTGCAGAGAA+AGG | 0.489526 | 3.4:-98246992 | None:intergenic |
| AACCAAAATCTGAGGGAAGA+AGG | 0.495894 | 3.4:+98246753 | MS.gene064796:CDS |
| TGATCCCTACCTGGACTGAA+TGG | 0.498481 | 3.4:-98246494 | None:intergenic |
| CACCAAGAGGTGCAGAGAAA+GGG | 0.499821 | 3.4:-98246991 | None:intergenic |
| CAAAAGATGTTATTTCATGT+CGG | 0.505412 | 3.4:+98246728 | MS.gene064796:CDS |
| AGATAAGAAGTATGCAATTG+AGG | 0.507400 | 3.4:+98246799 | MS.gene064796:CDS |
| CATAGATTAATTTCTTTACG+TGG | 0.529657 | 3.4:-98247055 | None:intergenic |
| ATCAAGACCTCATGGTGTCA+AGG | 0.530656 | 3.4:+98246520 | MS.gene064796:CDS |
| CAAAACCATCCATTCAGTCC+AGG | 0.540895 | 3.4:+98246485 | MS.gene064796:CDS |
| TCAAGACCTCATGGTGTCAA+GGG | 0.542315 | 3.4:+98246521 | MS.gene064796:CDS |
| TGATGATGATGAAGAAGAAG+AGG | 0.542344 | 3.4:+98246958 | MS.gene064796:CDS |
| CCCTACCTGGACTGAATGGA+TGG | 0.542832 | 3.4:-98246490 | None:intergenic |
| AAATTAATCTATGGAAGAGA+AGG | 0.544809 | 3.4:+98247065 | MS.gene064796:CDS |
| CCATCCATTCAGTCCAGGTA+GGG | 0.567822 | 3.4:+98246490 | MS.gene064796:CDS |
| TAGAGGCCTAGGTGGAGCCA+TGG | 0.570574 | 3.4:-98247089 | None:intergenic |
| TTTACTGGATCCAGTTGTAG+AGG | 0.574544 | 3.4:-98247106 | None:intergenic |
| GCAACACCACCACCACCAAG+AGG | 0.576743 | 3.4:-98247004 | None:intergenic |
| TGTCGGGAAACCAAAATCTG+AGG | 0.596722 | 3.4:+98246745 | MS.gene064796:CDS |
| GCATGGGACAAATCAAGCAC+AGG | 0.597529 | 3.4:+98246648 | MS.gene064796:CDS |
| GTCGGGAAACCAAAATCTGA+GGG | 0.603177 | 3.4:+98246746 | MS.gene064796:CDS |
| TCCAGTTGTAGAGGCCTAGG+TGG | 0.615336 | 3.4:-98247097 | None:intergenic |
| GATCACAAATCAAGACCTCA+TGG | 0.618438 | 3.4:+98246512 | MS.gene064796:CDS |
| GAAGGACCATGGCTCCACCT+AGG | 0.627085 | 3.4:+98247083 | MS.gene064796:CDS |
| GAAGGCTCAGGTAGCACCAG+AGG | 0.628886 | 3.4:+98246892 | MS.gene064796:CDS |
| GAAAATCCCTTGACACCATG+AGG | 0.634828 | 3.4:-98246527 | None:intergenic |
| ACCATCCATTCAGTCCAGGT+AGG | 0.635976 | 3.4:+98246489 | MS.gene064796:CDS |
| TCTATGGAAGAGAAGGACCA+TGG | 0.674583 | 3.4:+98247072 | MS.gene064796:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATTCCATTAATATCTT+TGG | - | chr3.4:98246597-98246616 | None:intergenic | 20.0% |
| !!! | AAGTTTATAGTGATAGTTTT+TGG | - | chr3.4:98246464-98246483 | None:intergenic | 20.0% |
| ! | AAATTAATCTATGGAAGAGA+AGG | + | chr3.4:98247065-98247084 | MS.gene064796:CDS | 25.0% |
| ! | AGAAAGTCCAAAGATATTAA+TGG | + | chr3.4:98246587-98246606 | MS.gene064796:CDS | 25.0% |
| ! | CACGTAAAGAAATTAATCTA+TGG | + | chr3.4:98247056-98247075 | MS.gene064796:CDS | 25.0% |
| ! | CATAGATTAATTTCTTTACG+TGG | - | chr3.4:98247058-98247077 | None:intergenic | 25.0% |
| ! | TAGTAATTATCTATTTCCTC+TGG | - | chr3.4:98246911-98246930 | None:intergenic | 25.0% |
| !!! | AAAAGATGTTATTTCATGTC+GGG | + | chr3.4:98246729-98246748 | MS.gene064796:CDS | 25.0% |
| !!! | ACACTTTTGCTAATTTTGAT+TGG | + | chr3.4:98246870-98246889 | MS.gene064796:CDS | 25.0% |
| !!! | CAAAAGATGTTATTTCATGT+CGG | + | chr3.4:98246728-98246747 | MS.gene064796:CDS | 25.0% |
| !!! | TTTTGCTAATTTTGATTGGA+AGG | + | chr3.4:98246874-98246893 | MS.gene064796:CDS | 25.0% |
| ! | AGATAAGAAGTATGCAATTG+AGG | + | chr3.4:98246799-98246818 | MS.gene064796:CDS | 30.0% |
| AGATCTTGTTCCTTAGCTTA+AGG | - | chr3.4:98246430-98246449 | None:intergenic | 35.0% | |
| TCACCAAAAATATCGTGCAT+GGG | + | chr3.4:98246632-98246651 | MS.gene064796:CDS | 35.0% | |
| TGGAGAAAAACCTTAAGCTA+AGG | + | chr3.4:98246417-98246436 | MS.gene064796:CDS | 35.0% | |
| TTCACCAAAAATATCGTGCA+TGG | + | chr3.4:98246631-98246650 | MS.gene064796:CDS | 35.0% | |
| ! | CAAATGAAACGTTTTGCAAG+TGG | + | chr3.4:98246842-98246861 | MS.gene064796:CDS | 35.0% |
| ! | TGATGATGATGAAGAAGAAG+AGG | + | chr3.4:98246958-98246977 | MS.gene064796:CDS | 35.0% |
| !!! | CACGATATTTTTGGTGAAGT+TGG | - | chr3.4:98246629-98246648 | None:intergenic | 35.0% |
| !!! | TAATTTTGATTGGAAGGCTC+AGG | + | chr3.4:98246880-98246899 | MS.gene064796:CDS | 35.0% |
| AACCAAAATCTGAGGGAAGA+AGG | + | chr3.4:98246753-98246772 | MS.gene064796:CDS | 40.0% | |
| GATCACAAATCAAGACCTCA+TGG | + | chr3.4:98246512-98246531 | MS.gene064796:CDS | 40.0% | |
| TGTGGAATCATGGATGCAAT+TGG | - | chr3.4:98246557-98246576 | None:intergenic | 40.0% | |
| TTTACTGGATCCAGTTGTAG+AGG | - | chr3.4:98247109-98247128 | None:intergenic | 40.0% | |
| ! | TCTAGCTTCATGTGGAATCA+TGG | - | chr3.4:98246567-98246586 | None:intergenic | 40.0% |
| ! | TGTCCCATGCACGATATTTT+TGG | - | chr3.4:98246638-98246657 | None:intergenic | 40.0% |
| ! | TTCCTTCTTCCCTCAGATTT+TGG | - | chr3.4:98246758-98246777 | None:intergenic | 40.0% |
| !! | GACTTTCTTCTAGCTTCATG+TGG | - | chr3.4:98246575-98246594 | None:intergenic | 40.0% |
| CAAAACCATCCATTCAGTCC+AGG | + | chr3.4:98246485-98246504 | MS.gene064796:CDS | 45.0% | |
| GAAAATCCCTTGACACCATG+AGG | - | chr3.4:98246530-98246549 | None:intergenic | 45.0% | |
| GTCGGGAAACCAAAATCTGA+GGG | + | chr3.4:98246746-98246765 | MS.gene064796:CDS | 45.0% | |
| TCTATGGAAGAGAAGGACCA+TGG | + | chr3.4:98247072-98247091 | MS.gene064796:CDS | 45.0% | |
| TGTCGGGAAACCAAAATCTG+AGG | + | chr3.4:98246745-98246764 | MS.gene064796:CDS | 45.0% | |
| ! | ATCAAGACCTCATGGTGTCA+AGG | + | chr3.4:98246520-98246539 | MS.gene064796:CDS | 45.0% |
| ! | TCAAGACCTCATGGTGTCAA+GGG | + | chr3.4:98246521-98246540 | MS.gene064796:CDS | 45.0% |
| !! | CATGGTGTCAAGGGATTTTC+AGG | + | chr3.4:98246530-98246549 | MS.gene064796:CDS | 45.0% |
| !! | CTTGATTTGTGATCCCTACC+TGG | - | chr3.4:98246506-98246525 | None:intergenic | 45.0% |
| ACCATCCATTCAGTCCAGGT+AGG | + | chr3.4:98246489-98246508 | MS.gene064796:CDS | 50.0% | |
| ATCCCTTTCTCTGCACCTCT+TGG | + | chr3.4:98246989-98247008 | MS.gene064796:CDS | 50.0% | |
| CCATCCATTCAGTCCAGGTA+GGG | + | chr3.4:98246490-98246509 | MS.gene064796:CDS | 50.0% | |
| GCATGGGACAAATCAAGCAC+AGG | + | chr3.4:98246648-98246667 | MS.gene064796:CDS | 50.0% | |
| TGATCCCTACCTGGACTGAA+TGG | - | chr3.4:98246497-98246516 | None:intergenic | 50.0% | |
| ! | CACCAAGAGGTGCAGAGAAA+GGG | - | chr3.4:98246994-98247013 | None:intergenic | 50.0% |
| !! | GGTGTTGCTGGTTGTAGTCT+TGG | + | chr3.4:98247019-98247038 | MS.gene064796:CDS | 50.0% |
| CCCTACCTGGACTGAATGGA+TGG | - | chr3.4:98246493-98246512 | None:intergenic | 55.0% | |
| CCTTTCTCTGCACCTCTTGG+TGG | + | chr3.4:98246992-98247011 | MS.gene064796:CDS | 55.0% | |
| GGATCCAGTTGTAGAGGCCT+AGG | - | chr3.4:98247103-98247122 | None:intergenic | 55.0% | |
| TCCACCTAGGCCTCTACAAC+TGG | + | chr3.4:98247096-98247115 | MS.gene064796:CDS | 55.0% | |
| TCCAGTTGTAGAGGCCTAGG+TGG | - | chr3.4:98247100-98247119 | None:intergenic | 55.0% | |
| TTCTCTGCACCTCTTGGTGG+TGG | + | chr3.4:98246995-98247014 | MS.gene064796:CDS | 55.0% | |
| ! | CCACCAAGAGGTGCAGAGAA+AGG | - | chr3.4:98246995-98247014 | None:intergenic | 55.0% |
| GAAGGACCATGGCTCCACCT+AGG | + | chr3.4:98247083-98247102 | MS.gene064796:CDS | 60.0% | |
| GAAGGCTCAGGTAGCACCAG+AGG | + | chr3.4:98246892-98246911 | MS.gene064796:CDS | 60.0% | |
| GCAACACCACCACCACCAAG+AGG | - | chr3.4:98247007-98247026 | None:intergenic | 60.0% | |
| TAGAGGCCTAGGTGGAGCCA+TGG | - | chr3.4:98247092-98247111 | None:intergenic | 60.0% | |
| !! | CTTGGTGGTGGTGGTGTTGC+TGG | + | chr3.4:98247007-98247026 | MS.gene064796:CDS | 60.0% |
| !! | TCTGCACCTCTTGGTGGTGG+TGG | + | chr3.4:98246998-98247017 | MS.gene064796:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 98246416 | 98247132 | 98246416 | ID=MS.gene064796 |
| chr3.4 | mRNA | 98246416 | 98247132 | 98246416 | ID=MS.gene064796.t1;Parent=MS.gene064796 |
| chr3.4 | exon | 98246416 | 98247132 | 98246416 | ID=MS.gene064796.t1.exon1;Parent=MS.gene064796.t1 |
| chr3.4 | CDS | 98246416 | 98247132 | 98246416 | ID=cds.MS.gene064796.t1;Parent=MS.gene064796.t1 |
| Gene Sequence |
| Protein sequence |
