Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065981.t1 | XP_003629868.1 | 86.3 | 679 | 87 | 3 | 1 | 678 | 1 | 674 | 0.00E+00 | 1109.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065981.t1 | Q9ZP55 | 57.3 | 672 | 278 | 5 | 3 | 671 | 2 | 667 | 1.3e-208 | 727.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065981.t1 | G7L7C1 | 86.3 | 679 | 87 | 3 | 1 | 678 | 1 | 674 | 0.0e+00 | 1109.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene065981 | 0.822366 | 2.46E-53 | -1.69E-46 |
| MS.gene058823 | MS.gene065981 | 0.931423 | 3.86E-94 | -1.69E-46 |
| MS.gene058909 | MS.gene065981 | 0.801361 | 9.34E-49 | -1.69E-46 |
| MS.gene058912 | MS.gene065981 | 0.801599 | 8.34E-49 | -1.69E-46 |
| MS.gene060962 | MS.gene065981 | 0.804026 | 2.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene80193 | MS.gene065981 | PPI |
| MS.gene014901 | MS.gene065981 | PPI |
| MS.gene065981 | MS.gene32695 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065981.t1 | MTR_8g087770 | 86.303 | 679 | 87 | 3 | 1 | 678 | 1 | 674 | 0.0 | 1192 |
| MS.gene065981.t1 | MTR_8g087750 | 82.712 | 671 | 111 | 3 | 6 | 675 | 11 | 677 | 0.0 | 1129 |
| MS.gene065981.t1 | MTR_8g087763 | 88.444 | 225 | 26 | 0 | 374 | 598 | 4 | 228 | 5.89e-144 | 423 |
| MS.gene065981.t1 | MTR_4g093750 | 34.739 | 403 | 234 | 14 | 67 | 452 | 35 | 425 | 7.48e-43 | 165 |
| MS.gene065981.t1 | MTR_4g093750 | 40.559 | 286 | 148 | 9 | 67 | 337 | 35 | 313 | 6.79e-42 | 163 |
| MS.gene065981.t1 | MTR_2g009690 | 37.580 | 314 | 184 | 6 | 63 | 366 | 40 | 351 | 1.74e-41 | 162 |
| MS.gene065981.t1 | MTR_5g009150 | 30.128 | 468 | 291 | 15 | 69 | 518 | 35 | 484 | 2.85e-34 | 139 |
| MS.gene065981.t1 | MTR_1g032550 | 35.336 | 283 | 163 | 7 | 69 | 337 | 35 | 311 | 3.25e-33 | 135 |
| MS.gene065981.t1 | MTR_8g105580 | 35.461 | 282 | 163 | 6 | 69 | 337 | 35 | 310 | 1.08e-32 | 134 |
| MS.gene065981.t1 | MTR_8g105580 | 35.461 | 282 | 163 | 6 | 69 | 337 | 35 | 310 | 1.09e-32 | 134 |
| MS.gene065981.t1 | MTR_8g099550 | 33.956 | 321 | 178 | 10 | 69 | 369 | 34 | 340 | 9.11e-32 | 131 |
| MS.gene065981.t1 | MTR_7g451010 | 34.700 | 317 | 181 | 9 | 69 | 369 | 40 | 346 | 9.71e-32 | 131 |
| MS.gene065981.t1 | MTR_8g009510 | 35.220 | 318 | 178 | 10 | 69 | 369 | 40 | 346 | 1.27e-31 | 131 |
| MS.gene065981.t1 | MTR_4g098920 | 34.375 | 320 | 178 | 11 | 69 | 369 | 34 | 340 | 6.53e-31 | 128 |
| MS.gene065981.t1 | MTR_4g085860 | 76.316 | 76 | 17 | 1 | 206 | 280 | 2 | 77 | 7.10e-31 | 115 |
| MS.gene065981.t1 | MTR_8g027675 | 41.709 | 199 | 105 | 5 | 69 | 264 | 34 | 224 | 1.00e-30 | 128 |
| MS.gene065981.t1 | MTR_6g045107 | 75.000 | 76 | 18 | 1 | 206 | 280 | 2 | 77 | 6.06e-29 | 110 |
| MS.gene065981.t1 | MTR_8g009510 | 31.452 | 248 | 146 | 7 | 136 | 369 | 2 | 239 | 5.46e-17 | 85.1 |
| MS.gene065981.t1 | MTR_8g463270 | 29.258 | 229 | 147 | 5 | 54 | 269 | 27 | 253 | 8.64e-16 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065981.t1 | AT1G60500 | 57.292 | 672 | 278 | 5 | 3 | 671 | 2 | 667 | 0.0 | 779 |
| MS.gene065981.t1 | AT1G60530 | 72.069 | 290 | 73 | 1 | 18 | 307 | 19 | 300 | 4.34e-144 | 422 |
| MS.gene065981.t1 | AT2G14120 | 30.180 | 444 | 287 | 10 | 25 | 452 | 1 | 437 | 5.81e-40 | 157 |
| MS.gene065981.t1 | AT2G14120 | 30.180 | 444 | 287 | 10 | 25 | 452 | 1 | 437 | 8.71e-40 | 157 |
| MS.gene065981.t1 | AT2G14120 | 30.180 | 444 | 287 | 10 | 25 | 452 | 1 | 437 | 9.55e-40 | 157 |
| MS.gene065981.t1 | AT2G14120 | 34.125 | 337 | 204 | 6 | 25 | 346 | 1 | 334 | 1.12e-39 | 156 |
| MS.gene065981.t1 | AT4G33650 | 29.797 | 443 | 267 | 11 | 35 | 452 | 29 | 452 | 2.03e-39 | 155 |
| MS.gene065981.t1 | AT4G33650 | 29.797 | 443 | 267 | 11 | 35 | 452 | 29 | 452 | 2.08e-39 | 155 |
| MS.gene065981.t1 | AT1G14830 | 32.903 | 310 | 196 | 6 | 69 | 369 | 35 | 341 | 8.02e-34 | 137 |
| MS.gene065981.t1 | AT5G42080 | 30.258 | 466 | 293 | 13 | 69 | 518 | 34 | 483 | 6.83e-33 | 134 |
| MS.gene065981.t1 | AT5G42080 | 30.258 | 466 | 293 | 13 | 69 | 518 | 34 | 483 | 6.83e-33 | 134 |
| MS.gene065981.t1 | AT2G44590 | 24.307 | 613 | 406 | 19 | 69 | 665 | 35 | 605 | 8.71e-33 | 134 |
| MS.gene065981.t1 | AT3G60190 | 33.232 | 328 | 199 | 9 | 69 | 386 | 40 | 357 | 1.30e-32 | 134 |
| MS.gene065981.t1 | AT5G42080 | 34.505 | 313 | 187 | 8 | 69 | 369 | 34 | 340 | 4.65e-32 | 129 |
| MS.gene065981.t1 | AT5G42080 | 34.505 | 313 | 187 | 8 | 69 | 369 | 34 | 340 | 1.23e-31 | 130 |
| MS.gene065981.t1 | AT3G61760 | 34.472 | 322 | 175 | 12 | 69 | 369 | 34 | 340 | 4.52e-30 | 124 |
| MS.gene065981.t1 | AT3G61760 | 34.472 | 322 | 175 | 12 | 69 | 369 | 34 | 340 | 4.93e-30 | 126 |
| MS.gene065981.t1 | AT3G61760 | 34.472 | 322 | 175 | 12 | 69 | 369 | 34 | 340 | 6.00e-30 | 125 |
| MS.gene065981.t1 | AT2G44590 | 22.838 | 613 | 398 | 20 | 69 | 665 | 35 | 588 | 4.72e-23 | 104 |
| MS.gene065981.t1 | AT2G44590 | 28.875 | 329 | 195 | 10 | 69 | 386 | 35 | 335 | 3.09e-22 | 102 |
| MS.gene065981.t1 | AT1G53140 | 27.119 | 236 | 160 | 5 | 44 | 269 | 36 | 269 | 9.77e-15 | 78.6 |
Find 125 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACTTTCAACAAAATTAAT+TGG | 0.173861 | 4.3:-13267721 | None:intergenic |
| CTTGAGGAAGCCAAATTTAT+TGG | 0.191059 | 4.3:+13267625 | MS.gene065981:CDS |
| ATTCGGAAGACCAATAAATT+TGG | 0.194065 | 4.3:-13267635 | None:intergenic |
| ATGGACAGGATTGGAAATTA+TGG | 0.215755 | 4.3:+13268351 | MS.gene065981:CDS |
| ATCAAGTTTGTTTGCCTTTG+TGG | 0.244719 | 4.3:-13266369 | None:intergenic |
| TATTGTTGGAATTCCTGTTT+TGG | 0.276334 | 4.3:+13267255 | MS.gene065981:CDS |
| GAGGAGTCTCCTTCAATATC+AGG | 0.303692 | 4.3:+13268261 | MS.gene065981:CDS |
| ATCTGATCATAAATGTTATC+AGG | 0.304162 | 4.3:-13266908 | None:intergenic |
| AGGCCAGATTGACAGCTTAT+TGG | 0.313028 | 4.3:+13268097 | MS.gene065981:CDS |
| TTCTAACTATTATCAGCTTC+AGG | 0.313313 | 4.3:+13267792 | MS.gene065981:CDS |
| GAACTTTGATTTCCTCCATT+AGG | 0.316135 | 4.3:-13267603 | None:intergenic |
| TAACATTTATGATCAGATTA+AGG | 0.317964 | 4.3:+13266913 | MS.gene065981:CDS |
| CTTCCAACCATTGTTGTTGT+TGG | 0.322456 | 4.3:+13266551 | MS.gene065981:CDS |
| AGAGACCGTGGCCAACATTA+TGG | 0.325375 | 4.3:+13268332 | MS.gene065981:CDS |
| TCACAAACTGTTGATAAAAC+TGG | 0.334047 | 4.3:+13267031 | MS.gene065981:CDS |
| TCCACAGCCAGAGCTTGTTT+TGG | 0.338480 | 4.3:+13266691 | MS.gene065981:CDS |
| CCTTACTTCAGAGAAAAGTT+AGG | 0.351453 | 4.3:+13267683 | MS.gene065981:CDS |
| TGAAAGTGTATGGAATTATC+TGG | 0.352378 | 4.3:+13267735 | MS.gene065981:CDS |
| CTTACTATGGTTGATCTTCC+AGG | 0.352429 | 4.3:+13266857 | MS.gene065981:CDS |
| TAAGAAGATTAATGAGAAAC+TGG | 0.360082 | 4.3:+13267336 | MS.gene065981:CDS |
| CAAGGGTACCTTTGGTTATG+AGG | 0.366182 | 4.3:+13266651 | MS.gene065981:CDS |
| TGGACAGGATTGGAAATTAT+GGG | 0.366857 | 4.3:+13268352 | MS.gene065981:CDS |
| CACATGTAACCCTGAGTACT+TGG | 0.369075 | 4.3:+13267915 | MS.gene065981:CDS |
| GCAGTTCTTGGCATGAAATT+CGG | 0.376721 | 4.3:-13267652 | None:intergenic |
| GCCTGCTAATTTATCCTCAC+TGG | 0.379029 | 4.3:+13267387 | MS.gene065981:CDS |
| TGGAGTTCCTGATCTTACTA+TGG | 0.379945 | 4.3:+13266844 | MS.gene065981:CDS |
| GCAGCCACTGAGGAGCTTGC+TGG | 0.382789 | 4.3:+13266770 | MS.gene065981:CDS |
| CTCCATTAGGAAATCCTTAG+TGG | 0.385218 | 4.3:-13267590 | None:intergenic |
| TAAATCTCCTGAAGGTTTGC+TGG | 0.387244 | 4.3:+13267087 | MS.gene065981:CDS |
| TTCAAAATAATACTCTCTTC+AGG | 0.392099 | 4.3:-13266959 | None:intergenic |
| GCTCTGGCTGTGGAAGTAAA+TGG | 0.394731 | 4.3:-13266682 | None:intergenic |
| AACAATAGATTTGTCTATCC+TGG | 0.395168 | 4.3:-13267239 | None:intergenic |
| ATAACTCGTGTTCCTGTTCA+TGG | 0.396246 | 4.3:+13266881 | MS.gene065981:CDS |
| ACCTTCTCCAGCAAACCTTC+AGG | 0.399453 | 4.3:-13267094 | None:intergenic |
| TTTCCAATAAGCTGTCAATC+TGG | 0.399717 | 4.3:-13268100 | None:intergenic |
| TTACTACTTGTGAGTCGATA+AGG | 0.405010 | 4.3:+13267005 | MS.gene065981:CDS |
| TGTCTCTTCCCTGATATTGA+AGG | 0.410432 | 4.3:-13268270 | None:intergenic |
| TTAATCTTCTTAATGATCTC+TGG | 0.413053 | 4.3:-13267325 | None:intergenic |
| GACGATGTTAACATTGGTCT+TGG | 0.414913 | 4.3:+13267124 | MS.gene065981:CDS |
| TTGATGCTCTTGAAAATCTA+AGG | 0.416842 | 4.3:+13266498 | MS.gene065981:CDS |
| AATTTGTACAAGGGTACCTT+TGG | 0.424069 | 4.3:+13266643 | MS.gene065981:CDS |
| AAGGTCAGAAAAGCAGTTCT+TGG | 0.424321 | 4.3:-13267664 | None:intergenic |
| GTCTTGGTTATGTTTGTGTA+AGG | 0.435750 | 4.3:+13267140 | MS.gene065981:CDS |
| ATATCCACACATGTTGAATC+AGG | 0.440980 | 4.3:+13268062 | MS.gene065981:CDS |
| TGGGGTGTTGGAAATCCCTT+TGG | 0.443439 | 4.3:-13266798 | None:intergenic |
| CTTACTTCAGAGAAAAGTTA+GGG | 0.447328 | 4.3:+13267684 | MS.gene065981:CDS |
| GGCTACTAGTTTGCATGAGC+TGG | 0.448592 | 4.3:+13267357 | MS.gene065981:CDS |
| AGGATAGACAAATCTATTGT+TGG | 0.453655 | 4.3:+13267241 | MS.gene065981:CDS |
| TCATTATAAGAAGATACAAT+TGG | 0.458646 | 4.3:-13266458 | None:intergenic |
| GGAAGAGACAGAAGTTGAGC+AGG | 0.460748 | 4.3:+13268283 | MS.gene065981:CDS |
| GAATCAGGCTTATGATTTGA+AGG | 0.463037 | 4.3:+13268077 | MS.gene065981:CDS |
| ACAATTGGTGCAACAGATAG+AGG | 0.467988 | 4.3:-13266443 | None:intergenic |
| TCTTTACTATCAAAGTCAGT+GGG | 0.470875 | 4.3:-13266817 | None:intergenic |
| TTATACTCCTCCAAGTACTC+AGG | 0.473801 | 4.3:-13267925 | None:intergenic |
| TGATACCAGCTAATGATTCA+AGG | 0.474056 | 4.3:-13266601 | None:intergenic |
| GAGCTTGCTGGCACTGCCAA+AGG | 0.478600 | 4.3:+13266782 | MS.gene065981:CDS |
| TCCTGAAGGTTTGCTGGAGA+AGG | 0.484851 | 4.3:+13267093 | MS.gene065981:CDS |
| GTTGTTGGAGATCAATCATC+TGG | 0.485196 | 4.3:+13266566 | MS.gene065981:CDS |
| AGCTTGAAGCACATGCTGAA+TGG | 0.486139 | 4.3:-13267866 | None:intergenic |
| TTATACTCCAAAACAAGCTC+TGG | 0.492061 | 4.3:-13266698 | None:intergenic |
| TATCAAAGTCAGTGGGGTGT+TGG | 0.492352 | 4.3:-13266810 | None:intergenic |
| TCCAATCCTGTCCATAATGT+TGG | 0.494024 | 4.3:-13268343 | None:intergenic |
| TTATCAGGTTGACCATGAAC+AGG | 0.495576 | 4.3:-13266893 | None:intergenic |
| GCGTCTCAACATTGCTAAAG+AGG | 0.501157 | 4.3:+13266520 | MS.gene065981:CDS |
| TGCCACTAAGGATTTCCTAA+TGG | 0.506730 | 4.3:+13267588 | MS.gene065981:CDS |
| TGTGTGGATATTTCATAAGC+TGG | 0.507052 | 4.3:-13268050 | None:intergenic |
| GTTTGTGTAAGGAATCGAAT+TGG | 0.512590 | 4.3:+13267151 | MS.gene065981:CDS |
| CTTATTGGAAAATTGTGCTC+CGG | 0.520290 | 4.3:+13268112 | MS.gene065981:CDS |
| GCCAGTGAGGATAAATTAGC+AGG | 0.522213 | 4.3:-13267388 | None:intergenic |
| AGATCAACCATAGTAAGATC+AGG | 0.522883 | 4.3:-13266851 | None:intergenic |
| AGTGTCCTTGAATCATTAGC+TGG | 0.523168 | 4.3:+13266596 | MS.gene065981:CDS |
| TCCAAAACAAGCTCTGGCTG+TGG | 0.524796 | 4.3:-13266692 | None:intergenic |
| GGTGCAACAGATAGAGGTTG+AGG | 0.525331 | 4.3:-13266437 | None:intergenic |
| TGTTGAAAGTAATGCCACTA+AGG | 0.528407 | 4.3:+13267576 | MS.gene065981:CDS |
| GCCAACATTATGGACAGGAT+TGG | 0.530993 | 4.3:+13268342 | MS.gene065981:CDS |
| AATGAACTGTTATCAGCAAA+TGG | 0.537861 | 4.3:+13268216 | MS.gene065981:CDS |
| ATCAGCTTGCCACGTGGACA+AGG | 0.540401 | 4.3:+13266620 | MS.gene065981:CDS |
| TCAGATTAAGGATATTATCA+TGG | 0.543580 | 4.3:+13266925 | MS.gene065981:CDS |
| GGAGGAAATCAAAGTTCTTG+AGG | 0.547046 | 4.3:+13267609 | MS.gene065981:CDS |
| AGCTTGCTGGCACTGCCAAA+GGG | 0.547647 | 4.3:+13266783 | MS.gene065981:CDS |
| TCTCCAACAACAACAATGGT+TGG | 0.551269 | 4.3:-13266554 | None:intergenic |
| TGTGAATGTTGATCTGCAAA+AGG | 0.555960 | 4.3:+13268185 | MS.gene065981:CDS |
| AGTGTCACACACTTCTGTCC+AGG | 0.565122 | 4.3:+13267221 | MS.gene065981:CDS |
| ACTTTGATAGTAAAGAAGAA+TGG | 0.569006 | 4.3:+13266824 | MS.gene065981:CDS |
| TCAAGTACTGTGAAGCTGAA+AGG | 0.569995 | 4.3:+13268006 | MS.gene065981:CDS |
| CACTAAGGATTTCCTAATGG+AGG | 0.576778 | 4.3:+13267591 | MS.gene065981:CDS |
| AGTGCCAGCAAGCTCCTCAG+TGG | 0.578957 | 4.3:-13266774 | None:intergenic |
| TTCTTTACTATCAAAGTCAG+TGG | 0.579933 | 4.3:-13266818 | None:intergenic |
| TATACTCCTCCAAGTACTCA+GGG | 0.581284 | 4.3:-13267924 | None:intergenic |
| CCGTGGCCAACATTATGGAC+AGG | 0.581354 | 4.3:+13268337 | MS.gene065981:CDS |
| TGAAAAGAAAATGCATTGCA+CGG | 0.584106 | 4.3:+13267504 | MS.gene065981:CDS |
| CAATCACATCAATTAACCTC+CGG | 0.585742 | 4.3:-13268131 | None:intergenic |
| CGTCTCAACATTGCTAAAGA+GGG | 0.585949 | 4.3:+13266521 | MS.gene065981:CDS |
| AGGAGTCTCCTTCAATATCA+GGG | 0.586223 | 4.3:+13268262 | MS.gene065981:CDS |
| ATGTAACCCTGAGTACTTGG+AGG | 0.589927 | 4.3:+13267918 | MS.gene065981:CDS |
| CCTGTCCATAATGTTGGCCA+CGG | 0.590976 | 4.3:-13268337 | None:intergenic |
| GTGGACAAGGAATTTGTACA+AGG | 0.592993 | 4.3:+13266633 | MS.gene065981:CDS |
| GGACAGGATTGGAAATTATG+GGG | 0.597404 | 4.3:+13268353 | MS.gene065981:CDS |
| AAGGCTGATAAATCTCCTGA+AGG | 0.599516 | 4.3:+13267079 | MS.gene065981:CDS |
| CAAACAACTTTCAACTGATG+AGG | 0.601066 | 4.3:+13266724 | MS.gene065981:CDS |
| AACGCTTGCTGTCGTGACAA+AGG | 0.611997 | 4.3:+13267060 | MS.gene065981:CDS |
| ACAGGAACACGAGTTATACC+TGG | 0.615115 | 4.3:-13266875 | None:intergenic |
| TGGTGTTGAGAGGCTGCTTG+AGG | 0.618613 | 4.3:+13268242 | MS.gene065981:CDS |
| TGTGCTTCAAGCTGTTGAGA+TGG | 0.619725 | 4.3:+13267876 | MS.gene065981:CDS |
| TAAGCCTGATTCAACATGTG+TGG | 0.620737 | 4.3:-13268066 | None:intergenic |
| ATTGGAAAATTGTGCTCCGG+AGG | 0.621567 | 4.3:+13268115 | MS.gene065981:CDS |
| GGAGGAGTATAACAAGCTGA+TGG | 0.621625 | 4.3:+13267936 | MS.gene065981:CDS |
| TGCTATAAACGCAGCCACTG+AGG | 0.623567 | 4.3:+13266760 | MS.gene065981:CDS |
| ACAGCTGACGATGTTAACAT+TGG | 0.623909 | 4.3:+13267118 | MS.gene065981:CDS |
| AAGAGTCTTACGAAGATGCA+AGG | 0.624326 | 4.3:+13267176 | MS.gene065981:CDS |
| GATTTGCAGAAAAGCAGACA+TGG | 0.624535 | 4.3:-13267416 | None:intergenic |
| CTGTTATCAGCAAATGGTGG+TGG | 0.626367 | 4.3:+13268222 | MS.gene065981:CDS |
| CAAATGGTGGTGGTGTTGAG+AGG | 0.637671 | 4.3:+13268232 | MS.gene065981:CDS |
| ACTGTGAAGCTGAAAGGTGT+TGG | 0.640769 | 4.3:+13268012 | MS.gene065981:CDS |
| GAAAGAAAGCAAAGAGACCG+TGG | 0.645229 | 4.3:+13268320 | MS.gene065981:CDS |
| TTGATCTCCAACAACAACAA+TGG | 0.648908 | 4.3:-13266558 | None:intergenic |
| GTACAAATTCCTTGTCCACG+TGG | 0.660130 | 4.3:-13266629 | None:intergenic |
| AGACATGGCATCAGCCAGTG+AGG | 0.673951 | 4.3:-13267401 | None:intergenic |
| CTTTACTATCAAAGTCAGTG+GGG | 0.697240 | 4.3:-13266816 | None:intergenic |
| GCTGGTATCAGCTTGCCACG+TGG | 0.699523 | 4.3:+13266614 | MS.gene065981:CDS |
| GTTGATCATCAACATCAACA+TGG | 0.706155 | 4.3:-13266415 | None:intergenic |
| TGGAAACAAGAGTTCCACAA+AGG | 0.707464 | 4.3:+13266355 | MS.gene065981:CDS |
| TTACTTCAGAGAAAAGTTAG+GGG | 0.725881 | 4.3:+13267685 | MS.gene065981:CDS |
| TGGACAAGGAATTTGTACAA+GGG | 0.728664 | 4.3:+13266634 | MS.gene065981:CDS |
| GAACTGTTATCAGCAAATGG+TGG | 0.747464 | 4.3:+13268219 | MS.gene065981:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACACTTTCAACAAAATTAAT+TGG | - | chr4.3:13267724-13267743 | None:intergenic | 20.0% |
| !! | CTGAAAAAAATTCTTCTTAT+AGG | + | chr4.3:13267460-13267479 | MS.gene065981:CDS | 20.0% |
| !! | TAACATTTATGATCAGATTA+AGG | + | chr4.3:13266913-13266932 | MS.gene065981:CDS | 20.0% |
| !! | TCATTATAAGAAGATACAAT+TGG | - | chr4.3:13266461-13266480 | None:intergenic | 20.0% |
| !!! | TTAATTTTGTTGAAAGTGTA+TGG | + | chr4.3:13267725-13267744 | MS.gene065981:CDS | 20.0% |
| ! | ATCTGATCATAAATGTTATC+AGG | - | chr4.3:13266911-13266930 | None:intergenic | 25.0% |
| ! | TAAGAAGATTAATGAGAAAC+TGG | + | chr4.3:13267336-13267355 | MS.gene065981:CDS | 25.0% |
| ! | TCAGATTAAGGATATTATCA+TGG | + | chr4.3:13266925-13266944 | MS.gene065981:CDS | 25.0% |
| ! | TTAATCTTCTTAATGATCTC+TGG | - | chr4.3:13267328-13267347 | None:intergenic | 25.0% |
| ! | TTCAAAATAATACTCTCTTC+AGG | - | chr4.3:13266962-13266981 | None:intergenic | 25.0% |
| !! | ACTTTGATAGTAAAGAAGAA+TGG | + | chr4.3:13266824-13266843 | MS.gene065981:CDS | 25.0% |
| !! | TCTTTTTCTTCTTAGCAATA+AGG | - | chr4.3:13267843-13267862 | None:intergenic | 25.0% |
| AACAATAGATTTGTCTATCC+TGG | - | chr4.3:13267242-13267261 | None:intergenic | 30.0% | |
| AATGAACTGTTATCAGCAAA+TGG | + | chr4.3:13268216-13268235 | MS.gene065981:CDS | 30.0% | |
| AGGATAGACAAATCTATTGT+TGG | + | chr4.3:13267241-13267260 | MS.gene065981:CDS | 30.0% | |
| ATTCGGAAGACCAATAAATT+TGG | - | chr4.3:13267638-13267657 | None:intergenic | 30.0% | |
| CTTACTTCAGAGAAAAGTTA+GGG | + | chr4.3:13267684-13267703 | MS.gene065981:CDS | 30.0% | |
| TCTTTACTATCAAAGTCAGT+GGG | - | chr4.3:13266820-13266839 | None:intergenic | 30.0% | |
| TGAAAAGAAAATGCATTGCA+CGG | + | chr4.3:13267504-13267523 | MS.gene065981:CDS | 30.0% | |
| TTACTTCAGAGAAAAGTTAG+GGG | + | chr4.3:13267685-13267704 | MS.gene065981:CDS | 30.0% | |
| TTCTAACTATTATCAGCTTC+AGG | + | chr4.3:13267792-13267811 | MS.gene065981:CDS | 30.0% | |
| TTCTTTACTATCAAAGTCAG+TGG | - | chr4.3:13266821-13266840 | None:intergenic | 30.0% | |
| TTGATGCTCTTGAAAATCTA+AGG | + | chr4.3:13266498-13266517 | MS.gene065981:CDS | 30.0% | |
| ! | TATTGTTGGAATTCCTGTTT+TGG | + | chr4.3:13267255-13267274 | MS.gene065981:CDS | 30.0% |
| ! | TCACAAACTGTTGATAAAAC+TGG | + | chr4.3:13267031-13267050 | MS.gene065981:CDS | 30.0% |
| ! | TGAAAGTGTATGGAATTATC+TGG | + | chr4.3:13267735-13267754 | MS.gene065981:CDS | 30.0% |
| AGATCAACCATAGTAAGATC+AGG | - | chr4.3:13266854-13266873 | None:intergenic | 35.0% | |
| ATATCCACACATGTTGAATC+AGG | + | chr4.3:13268062-13268081 | MS.gene065981:CDS | 35.0% | |
| ATGGACAGGATTGGAAATTA+TGG | + | chr4.3:13268351-13268370 | MS.gene065981:CDS | 35.0% | |
| CAAACAACTTTCAACTGATG+AGG | + | chr4.3:13266724-13266743 | MS.gene065981:CDS | 35.0% | |
| CAATCACATCAATTAACCTC+CGG | - | chr4.3:13268134-13268153 | None:intergenic | 35.0% | |
| CCTTACTTCAGAGAAAAGTT+AGG | + | chr4.3:13267683-13267702 | MS.gene065981:CDS | 35.0% | |
| CTTATTGGAAAATTGTGCTC+CGG | + | chr4.3:13268112-13268131 | MS.gene065981:CDS | 35.0% | |
| CTTGAGGAAGCCAAATTTAT+TGG | + | chr4.3:13267625-13267644 | MS.gene065981:CDS | 35.0% | |
| CTTTACTATCAAAGTCAGTG+GGG | - | chr4.3:13266819-13266838 | None:intergenic | 35.0% | |
| GAATCAGGCTTATGATTTGA+AGG | + | chr4.3:13268077-13268096 | MS.gene065981:CDS | 35.0% | |
| GTCTTGGTTATGTTTGTGTA+AGG | + | chr4.3:13267140-13267159 | MS.gene065981:CDS | 35.0% | |
| GTTGATCATCAACATCAACA+TGG | - | chr4.3:13266418-13266437 | None:intergenic | 35.0% | |
| GTTTGTGTAAGGAATCGAAT+TGG | + | chr4.3:13267151-13267170 | MS.gene065981:CDS | 35.0% | |
| TGATACCAGCTAATGATTCA+AGG | - | chr4.3:13266604-13266623 | None:intergenic | 35.0% | |
| TGGACAAGGAATTTGTACAA+GGG | + | chr4.3:13266634-13266653 | MS.gene065981:CDS | 35.0% | |
| TGGACAGGATTGGAAATTAT+GGG | + | chr4.3:13268352-13268371 | MS.gene065981:CDS | 35.0% | |
| TGTGAATGTTGATCTGCAAA+AGG | + | chr4.3:13268185-13268204 | MS.gene065981:CDS | 35.0% | |
| TGTGTGGATATTTCATAAGC+TGG | - | chr4.3:13268053-13268072 | None:intergenic | 35.0% | |
| TGTTGAAAGTAATGCCACTA+AGG | + | chr4.3:13267576-13267595 | MS.gene065981:CDS | 35.0% | |
| TTACTACTTGTGAGTCGATA+AGG | + | chr4.3:13267005-13267024 | MS.gene065981:CDS | 35.0% | |
| TTATACTCCAAAACAAGCTC+TGG | - | chr4.3:13266701-13266720 | None:intergenic | 35.0% | |
| TTGATCTCCAACAACAACAA+TGG | - | chr4.3:13266561-13266580 | None:intergenic | 35.0% | |
| TTTCCAATAAGCTGTCAATC+TGG | - | chr4.3:13268103-13268122 | None:intergenic | 35.0% | |
| ! | ATCAAGTTTGTTTGCCTTTG+TGG | - | chr4.3:13266372-13266391 | None:intergenic | 35.0% |
| ! | GAACTTTGATTTCCTCCATT+AGG | - | chr4.3:13267606-13267625 | None:intergenic | 35.0% |
| !! | AATTTGTACAAGGGTACCTT+TGG | + | chr4.3:13266643-13266662 | MS.gene065981:CDS | 35.0% |
| !!! | CCTAACTTTTCTCTGAAGTA+AGG | - | chr4.3:13267686-13267705 | None:intergenic | 35.0% |
| !!! | GAGCTTGTTTTGGAGTATAA+TGG | + | chr4.3:13266701-13266720 | MS.gene065981:CDS | 35.0% |
| AAGAGTCTTACGAAGATGCA+AGG | + | chr4.3:13267176-13267195 | MS.gene065981:CDS | 40.0% | |
| AAGGCTGATAAATCTCCTGA+AGG | + | chr4.3:13267079-13267098 | MS.gene065981:CDS | 40.0% | |
| AAGGTCAGAAAAGCAGTTCT+TGG | - | chr4.3:13267667-13267686 | None:intergenic | 40.0% | |
| ACAATTGGTGCAACAGATAG+AGG | - | chr4.3:13266446-13266465 | None:intergenic | 40.0% | |
| ACAGCTGACGATGTTAACAT+TGG | + | chr4.3:13267118-13267137 | MS.gene065981:CDS | 40.0% | |
| AGGAGTCTCCTTCAATATCA+GGG | + | chr4.3:13268262-13268281 | MS.gene065981:CDS | 40.0% | |
| AGTGTCCTTGAATCATTAGC+TGG | + | chr4.3:13266596-13266615 | MS.gene065981:CDS | 40.0% | |
| ATAACTCGTGTTCCTGTTCA+TGG | + | chr4.3:13266881-13266900 | MS.gene065981:CDS | 40.0% | |
| CACTAAGGATTTCCTAATGG+AGG | + | chr4.3:13267591-13267610 | MS.gene065981:CDS | 40.0% | |
| CGTCTCAACATTGCTAAAGA+GGG | + | chr4.3:13266521-13266540 | MS.gene065981:CDS | 40.0% | |
| CTCCATTAGGAAATCCTTAG+TGG | - | chr4.3:13267593-13267612 | None:intergenic | 40.0% | |
| CTTACTATGGTTGATCTTCC+AGG | + | chr4.3:13266857-13266876 | MS.gene065981:CDS | 40.0% | |
| GAACTGTTATCAGCAAATGG+TGG | + | chr4.3:13268219-13268238 | MS.gene065981:CDS | 40.0% | |
| GATTTGCAGAAAAGCAGACA+TGG | - | chr4.3:13267419-13267438 | None:intergenic | 40.0% | |
| GCAGTTCTTGGCATGAAATT+CGG | - | chr4.3:13267655-13267674 | None:intergenic | 40.0% | |
| GGACAGGATTGGAAATTATG+GGG | + | chr4.3:13268353-13268372 | MS.gene065981:CDS | 40.0% | |
| GGAGGAAATCAAAGTTCTTG+AGG | + | chr4.3:13267609-13267628 | MS.gene065981:CDS | 40.0% | |
| GTGGACAAGGAATTTGTACA+AGG | + | chr4.3:13266633-13266652 | MS.gene065981:CDS | 40.0% | |
| GTTGTTGGAGATCAATCATC+TGG | + | chr4.3:13266566-13266585 | MS.gene065981:CDS | 40.0% | |
| TAAATCTCCTGAAGGTTTGC+TGG | + | chr4.3:13267087-13267106 | MS.gene065981:CDS | 40.0% | |
| TAAGCCTGATTCAACATGTG+TGG | - | chr4.3:13268069-13268088 | None:intergenic | 40.0% | |
| TATACTCCTCCAAGTACTCA+GGG | - | chr4.3:13267927-13267946 | None:intergenic | 40.0% | |
| TCCAATCCTGTCCATAATGT+TGG | - | chr4.3:13268346-13268365 | None:intergenic | 40.0% | |
| TCTCCAACAACAACAATGGT+TGG | - | chr4.3:13266557-13266576 | None:intergenic | 40.0% | |
| TGCCACTAAGGATTTCCTAA+TGG | + | chr4.3:13267588-13267607 | MS.gene065981:CDS | 40.0% | |
| TGGAAACAAGAGTTCCACAA+AGG | + | chr4.3:13266355-13266374 | MS.gene065981:CDS | 40.0% | |
| TGGAGTTCCTGATCTTACTA+TGG | + | chr4.3:13266844-13266863 | MS.gene065981:CDS | 40.0% | |
| TGTCTCTTCCCTGATATTGA+AGG | - | chr4.3:13268273-13268292 | None:intergenic | 40.0% | |
| TTATACTCCTCCAAGTACTC+AGG | - | chr4.3:13267928-13267947 | None:intergenic | 40.0% | |
| ! | ACAAGCTTTTGAGCCAAAAC+AGG | - | chr4.3:13267271-13267290 | None:intergenic | 40.0% |
| ! | CTTCCAACCATTGTTGTTGT+TGG | + | chr4.3:13266551-13266570 | MS.gene065981:CDS | 40.0% |
| ! | GACGATGTTAACATTGGTCT+TGG | + | chr4.3:13267124-13267143 | MS.gene065981:CDS | 40.0% |
| ! | TCAAGTACTGTGAAGCTGAA+AGG | + | chr4.3:13268006-13268025 | MS.gene065981:CDS | 40.0% |
| ! | TTTTGCAGCCTCATAACCAA+AGG | - | chr4.3:13266662-13266681 | None:intergenic | 40.0% |
| !! | TTATCAGGTTGACCATGAAC+AGG | - | chr4.3:13266896-13266915 | None:intergenic | 40.0% |
| !!! | TTTTCAAGAGCATCAAGCAC+TGG | - | chr4.3:13266494-13266513 | None:intergenic | 40.0% |
| ACAGGAACACGAGTTATACC+TGG | - | chr4.3:13266878-13266897 | None:intergenic | 45.0% | |
| AGCTTGAAGCACATGCTGAA+TGG | - | chr4.3:13267869-13267888 | None:intergenic | 45.0% | |
| AGGCCAGATTGACAGCTTAT+TGG | + | chr4.3:13268097-13268116 | MS.gene065981:CDS | 45.0% | |
| ATGTAACCCTGAGTACTTGG+AGG | + | chr4.3:13267918-13267937 | MS.gene065981:CDS | 45.0% | |
| ATTGGAAAATTGTGCTCCGG+AGG | + | chr4.3:13268115-13268134 | MS.gene065981:CDS | 45.0% | |
| CACATGTAACCCTGAGTACT+TGG | + | chr4.3:13267915-13267934 | MS.gene065981:CDS | 45.0% | |
| CTGTTATCAGCAAATGGTGG+TGG | + | chr4.3:13268222-13268241 | MS.gene065981:CDS | 45.0% | |
| GAAAGAAAGCAAAGAGACCG+TGG | + | chr4.3:13268320-13268339 | MS.gene065981:CDS | 45.0% | |
| GAGGAGTCTCCTTCAATATC+AGG | + | chr4.3:13268261-13268280 | MS.gene065981:CDS | 45.0% | |
| GCCAACATTATGGACAGGAT+TGG | + | chr4.3:13268342-13268361 | MS.gene065981:CDS | 45.0% | |
| GCCAGTGAGGATAAATTAGC+AGG | - | chr4.3:13267391-13267410 | None:intergenic | 45.0% | |
| GCCTGCTAATTTATCCTCAC+TGG | + | chr4.3:13267387-13267406 | MS.gene065981:CDS | 45.0% | |
| GCGTCTCAACATTGCTAAAG+AGG | + | chr4.3:13266520-13266539 | MS.gene065981:CDS | 45.0% | |
| GTACAAATTCCTTGTCCACG+TGG | - | chr4.3:13266632-13266651 | None:intergenic | 45.0% | |
| TATCAAAGTCAGTGGGGTGT+TGG | - | chr4.3:13266813-13266832 | None:intergenic | 45.0% | |
| TGTGCTTCAAGCTGTTGAGA+TGG | + | chr4.3:13267876-13267895 | MS.gene065981:CDS | 45.0% | |
| ! | ACTGTGAAGCTGAAAGGTGT+TGG | + | chr4.3:13268012-13268031 | MS.gene065981:CDS | 45.0% |
| ! | GGAGGAGTATAACAAGCTGA+TGG | + | chr4.3:13267936-13267955 | MS.gene065981:CDS | 45.0% |
| !! | CAAGGGTACCTTTGGTTATG+AGG | + | chr4.3:13266651-13266670 | MS.gene065981:CDS | 45.0% |
| ACCTTCTCCAGCAAACCTTC+AGG | - | chr4.3:13267097-13267116 | None:intergenic | 50.0% | |
| AGAGACCGTGGCCAACATTA+TGG | + | chr4.3:13268332-13268351 | MS.gene065981:CDS | 50.0% | |
| AGTGTCACACACTTCTGTCC+AGG | + | chr4.3:13267221-13267240 | MS.gene065981:CDS | 50.0% | |
| CCTGTCCATAATGTTGGCCA+CGG | - | chr4.3:13268340-13268359 | None:intergenic | 50.0% | |
| GCTCTGGCTGTGGAAGTAAA+TGG | - | chr4.3:13266685-13266704 | None:intergenic | 50.0% | |
| GGAAGAGACAGAAGTTGAGC+AGG | + | chr4.3:13268283-13268302 | MS.gene065981:CDS | 50.0% | |
| GGTGCAACAGATAGAGGTTG+AGG | - | chr4.3:13266440-13266459 | None:intergenic | 50.0% | |
| TGCTATAAACGCAGCCACTG+AGG | + | chr4.3:13266760-13266779 | MS.gene065981:CDS | 50.0% | |
| ! | GGCTACTAGTTTGCATGAGC+TGG | + | chr4.3:13267357-13267376 | MS.gene065981:CDS | 50.0% |
| ! | TCCAAAACAAGCTCTGGCTG+TGG | - | chr4.3:13266695-13266714 | None:intergenic | 50.0% |
| !! | AACGCTTGCTGTCGTGACAA+AGG | + | chr4.3:13267060-13267079 | MS.gene065981:CDS | 50.0% |
| !! | CAAATGGTGGTGGTGTTGAG+AGG | + | chr4.3:13268232-13268251 | MS.gene065981:CDS | 50.0% |
| !! | TCCACAGCCAGAGCTTGTTT+TGG | + | chr4.3:13266691-13266710 | MS.gene065981:CDS | 50.0% |
| !! | TCCTGAAGGTTTGCTGGAGA+AGG | + | chr4.3:13267093-13267112 | MS.gene065981:CDS | 50.0% |
| !! | TGGGGTGTTGGAAATCCCTT+TGG | - | chr4.3:13266801-13266820 | None:intergenic | 50.0% |
| AGACATGGCATCAGCCAGTG+AGG | - | chr4.3:13267404-13267423 | None:intergenic | 55.0% | |
| ATCAGCTTGCCACGTGGACA+AGG | + | chr4.3:13266620-13266639 | MS.gene065981:CDS | 55.0% | |
| CCGTGGCCAACATTATGGAC+AGG | + | chr4.3:13268337-13268356 | MS.gene065981:CDS | 55.0% | |
| ! | TGGTGTTGAGAGGCTGCTTG+AGG | + | chr4.3:13268242-13268261 | MS.gene065981:CDS | 55.0% |
| !! | AGCTTGCTGGCACTGCCAAA+GGG | + | chr4.3:13266783-13266802 | MS.gene065981:CDS | 55.0% |
| AGTGCCAGCAAGCTCCTCAG+TGG | - | chr4.3:13266777-13266796 | None:intergenic | 60.0% | |
| GCTGGTATCAGCTTGCCACG+TGG | + | chr4.3:13266614-13266633 | MS.gene065981:CDS | 60.0% | |
| !! | GAGCTTGCTGGCACTGCCAA+AGG | + | chr4.3:13266782-13266801 | MS.gene065981:CDS | 60.0% |
| GCAGCCACTGAGGAGCTTGC+TGG | + | chr4.3:13266770-13266789 | MS.gene065981:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13266347 | 13268383 | 13266347 | ID=MS.gene065981 |
| chr4.3 | mRNA | 13266347 | 13268383 | 13266347 | ID=MS.gene065981.t1;Parent=MS.gene065981 |
| chr4.3 | exon | 13266347 | 13268383 | 13266347 | ID=MS.gene065981.t1.exon1;Parent=MS.gene065981.t1 |
| chr4.3 | CDS | 13266347 | 13268383 | 13266347 | ID=cds.MS.gene065981.t1;Parent=MS.gene065981.t1 |
| Gene Sequence |
| Protein sequence |
