Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066346.t1 | XP_013458284.1 | 98.6 | 288 | 4 | 0 | 1 | 288 | 352 | 639 | 1.10E-149 | 539.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066346.t1 | Q9LRZ3 | 53.6 | 280 | 124 | 2 | 1 | 278 | 358 | 633 | 5.9e-75 | 282.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066346.t1 | A0A072V2I3 | 98.6 | 288 | 4 | 0 | 1 | 288 | 352 | 639 | 8.0e-150 | 539.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049876 | MS.gene066346 | 0.814044 | 1.88E-51 | -1.69E-46 |
| MS.gene050916 | MS.gene066346 | 0.822507 | 2.28E-53 | -1.69E-46 |
| MS.gene055555 | MS.gene066346 | 0.805236 | 1.47E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene066346 | 0.804034 | 2.62E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene066346 | 0.824824 | 6.55E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066346.t1 | MTR_4g120470 | 98.611 | 288 | 4 | 0 | 1 | 288 | 352 | 639 | 0.0 | 576 |
| MS.gene066346.t1 | MTR_4g120460 | 57.407 | 108 | 19 | 2 | 163 | 270 | 90 | 170 | 1.14e-26 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066346.t1 | AT3G16810 | 55.124 | 283 | 115 | 4 | 1 | 278 | 358 | 633 | 2.62e-102 | 312 |
Find 0 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAAACTATAGTTTATAT+TGG | + | 32540:32775-32794 | None:intergenic | 15.0% |
| !! | TTATTTACATCAATAAATTC+TGG | - | 32540:33508-33527 | MS.gene066346:CDS | 15.0% |
| !!! | TTGAAAATTTCTTTTCAAAT+CGG | - | 32540:33868-33887 | MS.gene066346:intron | 15.0% |
| !!! | TTTTAAAATATTCTCATTTC+AGG | - | 32540:32645-32664 | MS.gene066346:intron | 15.0% |
| !! | AAATAAATAGTCTTTATGCA+TGG | + | 32540:32906-32925 | None:intergenic | 20.0% |
| !! | AGGATAACTAAATTATTAGA+AGG | + | 32540:33393-33412 | None:intergenic | 20.0% |
| !! | TATAAAAAGACGAAAAAGAT+GGG | + | 32540:33106-33125 | None:intergenic | 20.0% |
| !! | TATAAAACACAAGTAATACA+TGG | + | 32540:33417-33436 | None:intergenic | 20.0% |
| !!! | AGTTTTATCTTCATTAACTT+CGG | + | 32540:33262-33281 | None:intergenic | 20.0% |
| !!! | CTGATTCAATTTTTTTTGAT+GGG | - | 32540:33480-33499 | MS.gene066346:CDS | 20.0% |
| !!! | GATTTGAAAAGAAATTTTCA+AGG | + | 32540:33869-33888 | None:intergenic | 20.0% |
| !!! | TTATGCATGGTTTTATTTAT+GGG | + | 32540:32893-32912 | None:intergenic | 20.0% |
| !!! | TTTATGCATGGTTTTATTTA+TGG | + | 32540:32894-32913 | None:intergenic | 20.0% |
| ! | AAAAGGGAAATGTGAATTAT+GGG | - | 32540:33953-33972 | MS.gene066346:intron | 25.0% |
| ! | AGAACTCCATTATCAATTAA+AGG | + | 32540:34160-34179 | None:intergenic | 25.0% |
| ! | CCATCAAAAAAAATTGAATC+AGG | + | 32540:33482-33501 | None:intergenic | 25.0% |
| ! | GTATAAAAAGACGAAAAAGA+TGG | + | 32540:33107-33126 | None:intergenic | 25.0% |
| ! | TAAAAGGGAAATGTGAATTA+TGG | - | 32540:33952-33971 | MS.gene066346:intron | 25.0% |
| ! | TAATGAAGATAAAACTTCTG+TGG | - | 32540:33266-33285 | MS.gene066346:intron | 25.0% |
| ! | TGCCATTGAATTTATCAAAA+AGG | + | 32540:33714-33733 | None:intergenic | 25.0% |
| ! | TTAATCTTATCATCCAAAGT+TGG | + | 32540:33777-33796 | None:intergenic | 25.0% |
| !! | GTTTTATCTTCATTAACTTC+GGG | + | 32540:33261-33280 | None:intergenic | 25.0% |
| !! | TATTTTATGTTCTTATGACG+TGG | - | 32540:32921-32940 | MS.gene066346:CDS | 25.0% |
| !! | TGCCTTTTTGATAAATTCAA+TGG | - | 32540:33709-33728 | MS.gene066346:CDS | 25.0% |
| !!! | CCTGATTCAATTTTTTTTGA+TGG | - | 32540:33479-33498 | MS.gene066346:CDS | 25.0% |
| !!! | GTTTTGATGAATATAACTTC+CGG | - | 32540:32952-32971 | MS.gene066346:CDS | 25.0% |
| !!! | TCAATTTTTTTTGATGGGAA+AGG | - | 32540:33485-33504 | MS.gene066346:CDS | 25.0% |
| AAACACAAGTAATACATGGA+AGG | + | 32540:33413-33432 | None:intergenic | 30.0% | |
| ATATGCAGTTAGCATCTATT+TGG | - | 32540:32828-32847 | MS.gene066346:CDS | 30.0% | |
| CGATACAAAACTGATAACAA+AGG | - | 32540:32553-32572 | MS.gene066346:CDS | 30.0% | |
| GACCATTAGAAAACTAATCT+TGG | - | 32540:33890-33909 | MS.gene066346:intron | 30.0% | |
| GATATATGCATTGAGAATGT+AGG | - | 32540:33587-33606 | MS.gene066346:intron | 30.0% | |
| TAGATGCTAACTGCATATAA+AGG | + | 32540:32826-32845 | None:intergenic | 30.0% | |
| TTGCAGCCTTTAATTGATAA+TGG | - | 32540:34151-34170 | MS.gene066346:intron | 30.0% | |
| ! | TAAGGTTATCACTGCATTTT+TGG | - | 32540:34087-34106 | MS.gene066346:intron | 30.0% |
| ! | TTTGGAATCAACAGATTCAA+AGG | - | 32540:34105-34124 | MS.gene066346:intron | 30.0% |
| !!! | GTCCAAGATTAGTTTTCTAA+TGG | + | 32540:33895-33914 | None:intergenic | 30.0% |
| AACAGTTGGGATGTAGTAAT+TGG | + | 32540:33006-33025 | None:intergenic | 35.0% | |
| AGAATGTAGGAGAACTGATA+CGG | - | 32540:33600-33619 | MS.gene066346:intron | 35.0% | |
| AGAGGTTAAGGAAAATACAG+AGG | + | 32540:33450-33469 | None:intergenic | 35.0% | |
| ATAAATTCAATGGCACATGC+AGG | - | 32540:33719-33738 | MS.gene066346:CDS | 35.0% | |
| ATCATCATCCAACAGAATCA+TGG | - | 32540:34010-34029 | MS.gene066346:intron | 35.0% | |
| GAAACTTCCAAGGTTTCAAT+TGG | - | 32540:33198-33217 | MS.gene066346:intron | 35.0% | |
| GGATGTAGTAATTGGAGTAA+AGG | + | 32540:32998-33017 | None:intergenic | 35.0% | |
| TAAGTCAGAAGATTCACAAG+AGG | - | 32540:33836-33855 | MS.gene066346:intron | 35.0% | |
| TCTTGTGAATCTTCTGACTT+AGG | + | 32540:33837-33856 | None:intergenic | 35.0% | |
| TTATGGGATTGAAGAAGCTA+GGG | + | 32540:32877-32896 | None:intergenic | 35.0% | |
| TTCATAGAAGCAAGTTCATC+AGG | + | 32540:33043-33062 | None:intergenic | 35.0% | |
| TTTATGGGATTGAAGAAGCT+AGG | + | 32540:32878-32897 | None:intergenic | 35.0% | |
| ! | GAACTGATACGGTCAAATTT+TGG | - | 32540:33611-33630 | MS.gene066346:intron | 35.0% |
| ! | TAACTTCCGGATTTTCAGAA+TGG | - | 32540:32965-32984 | MS.gene066346:CDS | 35.0% |
| ! | TCTGTATTTTCCTTAACCTC+TGG | - | 32540:33449-33468 | MS.gene066346:CDS | 35.0% |
| !! | AATTCTGAAGGAGCTTGTTT+TGG | - | 32540:32690-32709 | MS.gene066346:intron | 35.0% |
| !! | TGTGGAAGGAAGCTTTAAAA+GGG | - | 32540:33937-33956 | MS.gene066346:intron | 35.0% |
| !! | TTGTGGAAGGAAGCTTTAAA+AGG | - | 32540:33936-33955 | MS.gene066346:intron | 35.0% |
| AAATGTGAATTATGGGCCCA+TGG | - | 32540:33960-33979 | MS.gene066346:intron | 40.0% | |
| ACTTTGTGATTGCAGCTGTA+AGG | - | 32540:34069-34088 | MS.gene066346:intron | 40.0% | |
| CGAGCTTCAATCAATTCTGA+AGG | - | 32540:32678-32697 | MS.gene066346:intron | 40.0% | |
| TAAAGCTTCCTTCCACAAGA+TGG | + | 32540:33934-33953 | None:intergenic | 40.0% | |
| TATACCTTCTCTGTCTCTCA+AGG | - | 32540:33069-33088 | MS.gene066346:intron | 40.0% | |
| TTGTATCGTCGACAACTGAA+AGG | + | 32540:32541-32560 | None:intergenic | 40.0% | |
| ! | CGTCTTCCATTCTGAAAATC+CGG | + | 32540:32974-32993 | None:intergenic | 40.0% |
| ! | TTTCACTTTGCTGTCTTCCA+TGG | + | 32540:33235-33254 | None:intergenic | 40.0% |
| !! | ACGGTCAAATTTTGGCAAAG+AGG | - | 32540:33619-33638 | MS.gene066346:intron | 40.0% |
| !! | CATTCATTTTGATCGCTGTC+AGG | - | 32540:33149-33168 | MS.gene066346:intron | 40.0% |
| !! | CATTGGCTTGCTTGTTCTTT+GGG | + | 32540:34196-34215 | None:intergenic | 40.0% |
| !! | TCATTGGCTTGCTTGTTCTT+TGG | + | 32540:34197-34216 | None:intergenic | 40.0% |
| AATTGGCGACGATGATTCCA+TGG | - | 32540:33215-33234 | MS.gene066346:intron | 45.0% | |
| ACAAGATGGAAGCAAAGTCG+GGG | + | 32540:33920-33939 | None:intergenic | 45.0% | |
| ACTGAAGTAGCGTGAACAGT+TGG | + | 32540:33020-33039 | None:intergenic | 45.0% | |
| AGAAAGACCCATTGGTCAGA+AGG | - | 32540:33316-33335 | MS.gene066346:intron | 45.0% | |
| ATCGTCGACAACTGAAAGGA+TGG | + | 32540:32537-32556 | None:intergenic | 45.0% | |
| ATCGTCGCCAATTGAAACCT+TGG | + | 32540:33208-33227 | None:intergenic | 45.0% | |
| CAAGAGATGCTATCGCTTTG+TGG | + | 32540:33806-33825 | None:intergenic | 45.0% | |
| CACAAGATGGAAGCAAAGTC+GGG | + | 32540:33921-33940 | None:intergenic | 45.0% | |
| CAGGAGCTATTGATCAACAG+TGG | - | 32540:33339-33358 | MS.gene066346:intron | 45.0% | |
| CCAACAGAATCATGGTACAG+AGG | - | 32540:34018-34037 | MS.gene066346:intron | 45.0% | |
| CCACAAGATGGAAGCAAAGT+CGG | + | 32540:33922-33941 | None:intergenic | 45.0% | |
| CCTCTGTACCATGATTCTGT+TGG | + | 32540:34021-34040 | None:intergenic | 45.0% | |
| CTCTCAGACAGAAACTTCCA+AGG | - | 32540:33188-33207 | MS.gene066346:intron | 45.0% | |
| CTGAAGTAGCGTGAACAGTT+GGG | + | 32540:33019-33038 | None:intergenic | 45.0% | |
| CTTTCTTTCCACTCTCAGCT+AGG | + | 32540:33302-33321 | None:intergenic | 45.0% | |
| CTTTGCTTCCATCTTGTGGA+AGG | - | 32540:33923-33942 | MS.gene066346:intron | 45.0% | |
| GAAACCTTGAGAGACAGAGA+AGG | + | 32540:33076-33095 | None:intergenic | 45.0% | |
| GAGTGGAAAGAAAGACCCAT+TGG | - | 32540:33308-33327 | MS.gene066346:intron | 45.0% | |
| GGGCATATTGCATCCAACTT+TGG | - | 32540:33761-33780 | MS.gene066346:intron | 45.0% | |
| TGGCAAAGAGGTCATATACG+AGG | - | 32540:33631-33650 | MS.gene066346:intron | 45.0% | |
| TTGCTTGTTCTTTGGGCTGT+GGG | + | 32540:34189-34208 | None:intergenic | 45.0% | |
| !!! | GAAGGAGCTTGTTTTGGACA+AGG | - | 32540:32696-32715 | MS.gene066346:intron | 45.0% |
| AATTATGGGCCCATGGTCAC+AGG | - | 32540:33967-33986 | MS.gene066346:intron | 50.0% | |
| AGGTCAGCAACCAGAGGTTA+AGG | + | 32540:33462-33481 | None:intergenic | 50.0% | |
| ATGAGACCTACCTGTGACCA+TGG | + | 32540:33980-33999 | None:intergenic | 50.0% | |
| ATTGAAGAAGCTAGGGCAGG+AGG | + | 32540:32870-32889 | None:intergenic | 50.0% | |
| CCGACTTTGCTTCCATCTTG+TGG | - | 32540:33919-33938 | MS.gene066346:intron | 50.0% | |
| CTTGCTTGTTCTTTGGGCTG+TGG | + | 32540:34190-34209 | None:intergenic | 50.0% | |
| GATACTGACCTAGCTGAGAG+TGG | - | 32540:33291-33310 | MS.gene066346:intron | 50.0% | |
| TGAATCAGGTCAGCAACCAG+AGG | + | 32540:33468-33487 | None:intergenic | 50.0% | |
| TGAGACCTACCTGTGACCAT+GGG | + | 32540:33979-33998 | None:intergenic | 50.0% | |
| ! | AACAGTGGACTTGCAGAGGT+CGG | - | 32540:33354-33373 | MS.gene066346:intron | 50.0% |
| ! | GATCAACAGTGGACTTGCAG+AGG | - | 32540:33350-33369 | MS.gene066346:intron | 50.0% |
| AGACCCATTGGTCAGAAGGC+AGG | - | 32540:33320-33339 | MS.gene066346:intron | 55.0% | |
| AGCTCCTGCCTTCTGACCAA+TGG | + | 32540:33327-33346 | None:intergenic | 55.0% | |
| GCTCCTGCCTTCTGACCAAT+GGG | + | 32540:33326-33345 | None:intergenic | 55.0% | |
| GGGATTGAAGAAGCTAGGGC+AGG | + | 32540:32873-32892 | None:intergenic | 55.0% | |
| ATGGGCCCATGGTCACAGGT+AGG | - | 32540:33971-33990 | MS.gene066346:intron | 60.0% | |
| CACATGCAGGTTGCGACAGG+GGG | - | 32540:33732-33751 | MS.gene066346:CDS | 60.0% | |
| GCACATGCAGGTTGCGACAG+GGG | - | 32540:33731-33750 | MS.gene066346:CDS | 60.0% | |
| GGCACATGCAGGTTGCGACA+GGG | - | 32540:33730-33749 | MS.gene066346:CDS | 60.0% | |
| GTTGCGACAGGGGGATCTGA+GGG | - | 32540:33741-33760 | MS.gene066346:intron | 60.0% | |
| TGGCACATGCAGGTTGCGAC+AGG | - | 32540:33729-33748 | MS.gene066346:CDS | 60.0% | |
| GGTTGCGACAGGGGGATCTG+AGG | - | 32540:33740-33759 | MS.gene066346:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32540 | gene | 32524 | 34216 | 32524 | ID=MS.gene066346 |
| 32540 | mRNA | 32524 | 34216 | 32524 | ID=MS.gene066346.t1;Parent=MS.gene066346 |
| 32540 | exon | 34166 | 34216 | 34166 | ID=MS.gene066346.t1.exon1;Parent=MS.gene066346.t1 |
| 32540 | CDS | 34166 | 34216 | 34166 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 34023 | 34073 | 34023 | ID=MS.gene066346.t1.exon2;Parent=MS.gene066346.t1 |
| 32540 | CDS | 34023 | 34073 | 34023 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 33650 | 33757 | 33650 | ID=MS.gene066346.t1.exon3;Parent=MS.gene066346.t1 |
| 32540 | CDS | 33650 | 33757 | 33650 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 33369 | 33569 | 33369 | ID=MS.gene066346.t1.exon4;Parent=MS.gene066346.t1 |
| 32540 | CDS | 33369 | 33569 | 33369 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 33088 | 33162 | 33088 | ID=MS.gene066346.t1.exon5;Parent=MS.gene066346.t1 |
| 32540 | CDS | 33088 | 33162 | 33088 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 32752 | 32999 | 32752 | ID=MS.gene066346.t1.exon6;Parent=MS.gene066346.t1 |
| 32540 | CDS | 32752 | 32999 | 32752 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| 32540 | exon | 32524 | 32656 | 32524 | ID=MS.gene066346.t1.exon7;Parent=MS.gene066346.t1 |
| 32540 | CDS | 32524 | 32656 | 32524 | ID=cds.MS.gene066346.t1;Parent=MS.gene066346.t1 |
| Gene Sequence |
| Protein sequence |
