AlfalfaGEDB | MS.gene066599

M. sativa cultivar XinJiangDaYe / MS.gene066599

KEGG: K14638
GO: GO:0003674 GO:0005215 GO:0005488 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0030246 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00139.22 PF00854.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066599.t1	XP_024638218.1	72.3	455	14	2	1	343	125	579	2.80E-170	608.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066599.t1	P02867	58.2	213	80	3	337	540	61	273	2.2e-67	258.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066599.t1	A0A396I0T1	72.3	455	14	2	1	343	125	579	2.0e-170	608.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene066599	0.820964	5.19E-53	-1.69E-46
MS.gene050226	MS.gene066599	0.806478	8.07E-50	-1.69E-46
MS.gene050227	MS.gene066599	0.824947	6.12E-54	-1.69E-46
MS.gene050228	MS.gene066599	0.810607	1.06E-50	-1.69E-46
MS.gene050623	MS.gene066599	-0.817498	3.20E-52	-1.69E-46
MS.gene050824	MS.gene066599	0.807602	4.66E-50	-1.69E-46
MS.gene054379	MS.gene066599	0.813672	2.27E-51	-1.69E-46
MS.gene060137	MS.gene066599	-0.802335	5.89E-49	-1.69E-46
MS.gene060879	MS.gene066599	0.800295	1.54E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066599.t1	MTR_4g023810	94.146	205	12	0	337	541	61	265	2.51e-139	404
MS.gene066599.t1	MTR_8g023510	61.000	200	70	5	337	530	62	259	4.84e-78	247
MS.gene066599.t1	MTR_4g024930	63.125	160	39	2	203	343	395	553	7.28e-53	189
MS.gene066599.t1	MTR_4g024930	41.395	215	106	5	3	212	125	324	5.68e-33	133
MS.gene066599.t1	MTR_3g463370	43.694	222	101	6	327	531	9	223	2.97e-49	170
MS.gene066599.t1	MTR_7g010820	41.346	208	121	1	1	208	113	319	1.07e-46	172
MS.gene066599.t1	MTR_7g010820	42.308	156	65	1	206	336	407	562	2.46e-24	107
MS.gene066599.t1	MTR_5g014150	44.086	186	103	1	12	196	142	327	1.44e-46	172
MS.gene066599.t1	MTR_5g014150	41.143	175	78	3	205	355	427	600	2.13e-28	119
MS.gene066599.t1	MTR_4g098800	42.289	201	115	1	1	200	123	323	1.26e-45	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066599.t1	AT1G32450	43.979	191	103	2	8	194	131	321	7.46e-44	165
MS.gene066599.t1	AT1G32450	42.208	154	66	2	205	336	423	575	5.91e-28	118
MS.gene066599.t1	AT2G02020	29.668	391	214	11	1	336	129	513	1.60e-42	159
MS.gene066599.t1	AT4G21680	42.647	204	109	3	1	200	115	314	1.63e-40	155
MS.gene066599.t1	AT4G21680	40.268	149	65	2	212	337	418	565	2.05e-24	107
MS.gene066599.t1	AT2G02040	33.831	201	126	3	2	199	129	325	1.56e-31	129
MS.gene066599.t1	AT2G02040	34.839	155	77	2	212	343	426	579	4.14e-21	97.4
MS.gene066599.t1	AT5G19640	35.204	196	118	3	3	195	148	337	6.13e-31	127
MS.gene066599.t1	AT5G19640	42.069	145	66	1	211	337	432	576	5.04e-28	119
MS.gene066599.t1	AT1G62200	34.673	199	120	3	3	195	141	335	1.99e-29	122

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Find 105 sgRNAs with CRISPR-Local

Find 400 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTACATCAAGTGGTCA+AGG	0.554424	4.2:+79233986	MS.gene066599:CDS
AAACAAGTTACAACTCAACA+AGG	0.685986	4.2:+79237528	MS.gene066599:intron
AAACTATGCAGAAGCTTGCA+TGG	0.532882	4.2:-79238133	None:intergenic
AAATGACCGTCATTTAGATT+AGG	0.431490	4.2:-79234028	None:intergenic
AAATTTCAAATACAATCAAT+TGG	0.357133	4.2:+79232129	MS.gene066599:CDS
AACTGAAAAGTCACCTCCAG+TGG	0.600260	4.2:-79238058	None:intergenic
AAGATGGTGGAGGGTTTCTA+GGG	0.473640	4.2:+79237728	MS.gene066599:CDS
AAGCCAACCCTAATCCACTC+AGG	0.627564	4.2:-79238027	None:intergenic
AAGCTTGCATGGAGCTTGAA+TGG	0.516812	4.2:-79238122	None:intergenic
AAGTGTTACTTTATTTACTG+AGG	0.605839	4.2:+79237957	MS.gene066599:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTACATCAAGTGGTCA+AGG	+	chr4.2:79233986-79234005	MS.gene066599:CDS	30.0%
AAACAAGTTACAACTCAACA+AGG	+	chr4.2:79237528-79237547	MS.gene066599:intron	30.0%
AAATGACCGTCATTTAGATT+AGG	-	chr4.2:79234031-79234050	None:intergenic	30.0%
AACTCATTCATAGTCAATGT+AGG	-	chr4.2:79232964-79232983	None:intergenic	30.0%
AAGATTGTGCAAAACCATAT+TGG	-	chr4.2:79233455-79233474	None:intergenic	30.0%
AAGGCCAAAAATCATACAAA+TGG	+	chr4.2:79233375-79233394	MS.gene066599:intron	30.0%
AATGAGTTCACTGTTAACAA+AGG	+	chr4.2:79232976-79232995	MS.gene066599:intron	30.0%
ACAATAATACCTGGATACTA+TGG	+	chr4.2:79236020-79236039	MS.gene066599:intron	30.0%
ACACTTCAAACAATAATACC+TGG	+	chr4.2:79236011-79236030	MS.gene066599:intron	30.0%
AGTTGTGAAAACTTAGAGAA+AGG	-	chr4.2:79232084-79232103	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	79231673	79238152	79231673	ID=MS.gene066599
chr4.2	mRNA	79231673	79238152	79231673	ID=MS.gene066599.t1;Parent=MS.gene066599
chr4.2	exon	79231673	79232226	79231673	ID=MS.gene066599.t1.exon1;Parent=MS.gene066599.t1
chr4.2	CDS	79231673	79232226	79231673	ID=cds.MS.gene066599.t1;Parent=MS.gene066599.t1
chr4.2	exon	79233319	79233371	79233319	ID=MS.gene066599.t1.exon2;Parent=MS.gene066599.t1
chr4.2	CDS	79233319	79233371	79233319	ID=cds.MS.gene066599.t1;Parent=MS.gene066599.t1
chr4.2	exon	79233651	79233779	79233651	ID=MS.gene066599.t1.exon3;Parent=MS.gene066599.t1
chr4.2	CDS	79233651	79233779	79233651	ID=cds.MS.gene066599.t1;Parent=MS.gene066599.t1
chr4.2	exon	79233837	79234108	79233837	ID=MS.gene066599.t1.exon4;Parent=MS.gene066599.t1
chr4.2	CDS	79233837	79234108	79233837	ID=cds.MS.gene066599.t1;Parent=MS.gene066599.t1
chr4.2	exon	79237535	79238152	79237535	ID=MS.gene066599.t1.exon5;Parent=MS.gene066599.t1
chr4.2	CDS	79237535	79238152	79237535	ID=cds.MS.gene066599.t1;Parent=MS.gene066599.t1

Gene Sequence

>MS.gene066599

ATGGCGTTATCTTTGTCAACTCACTTCTTTTTACTCAAACCACATCGTTGTGAACAAATAGGAGAGTTATGTGAATCAGATACACAAAATCAGTTTCCACTTTTTTACCTATCAATATATCTAATAGCTCTAGGAAGTGGAGTTTCTGACCCTGCATTACCAACACTTGGTGCTGATCAATTTGATGAGGAAGAACCTAAAGAACAAAGATCCAAGGCCTCAATTTATGGCTACTTTTATGTGGCATTGAACTTGGGATCATTGGTTGCTGAGACTATATTGGCTTACATAGAGACTACAGGACATTGGGTGCTTGGATTCTGGATATGTACAAGTTGTGCTGCTGTTTCATTTCTTGTTTTGTTGAGTGGAACTCTTAGATACAGACACTATAAGACTTTTGGAAACCCTTTCTCTAAGTTTTCACAACTGATTGTACCCTTTTTGAAGAAAATGAAATTTCAAATACAATCAATTGGGGAAGATCTCTACCACGACGGTATTCAAGGGGATGATACTCGTGTTAGAAGAATACACCATGCAAATGGCCTCAGGTACGTCTCTTAAGAACAAGTAGAAGTGATTTTTATTTTTCTCGCATTTTTTTATAATCAAAATATTAAATTAAAACAAGTACAAGGGGCCAATGTAGATTGGTCTAGTTTCTAGGGATTCAACTCAGATCTCGCAAGGATCTGGGTTCAAACCCATATGCCAATGTGAGAACTTCATTGGCGAGTAATATGATCTCTTTGTCAATGCTATTTGAGCATTGAGGCTTTCCCTAGTCCTGATGTGCCCCCAGGACCCCATCCCGCCTAAACTTTTTCTCTACAAAAAAAAAAAAAGAAAGAAAAAGAAAATGAGTACAAGAGATACTCAAATTCAAATACAAGAAAACAAAAGTTCAAGAAAACTACAAGGACAAGTCCTCATACAAGCTATTACCCGTAATAGTAAACCAATTTCAAAAAAGCTTTGATGTGAGCTAACACTACTAGCATATTGAAAAGTTCAAGAAGTATAGCATTGCTTAGTTCAAAAATTGCTTTATATTCCTAAGGCCTTGATTTTGGTGGAGATTTGACGTCCAGGTCTTTTGGTGATTTTGATCATTTAGCTCATTGTTGCTTTTGTGATCTCCCGAACTCCTAAACAAGTGGGTATCATAGCCTTGTTTCGACTTGGTGTGAGAGCGTTAGTGGAATCCTAGTGTTTTGATCAAGTATAGTGATATGATATTCACACTTGAGGGCGAGTTTTGTTGGTATTCAAGTGTGAATGTAATCCTACATTGACTATGAATGAGTTCACTGTTAACAAAGGTGAGGTAACCCATATTCCCAAAGCTCTAAGATTTTGGATGAAAATGTGATATCCCGCCGAGTTCACTGTTATATAACAGTTTTGTTATTTGTAATTGTGAAAACCCTATATTTATAGAGTTGATTTGATATGACAAGAAAGAGTGAAAGATAGAAGCCATTCCATTCTTGTTAGCCATACAATCTGTTGCAAGTTGTCAAGTATTCTATGACTACTATTTTTTAGTTAAAGGATACAAACACGAGGAAAGCACCTTATAAAAAAAATTCTTTAAATAATCTCCAAATTTTCTTTATTCATTTTGTTTCGGTGACATACAGGTTTTTTGATAGAGCTGGTATTGTTTCTGATGAGGCAACTGAGATGTTACTAGGTAAAGGCCAAAAATCATACAAATGGAATTTTAACAGTCTAACACAAAGCGAAGCGGTGACATATATTTTGAGAGTGTTACCAATATGGTTTTGCACAATCTTCTCCTCTAGTGTTTTCATACAATTGCTTTCTCTATTTGTTGAGCAAGGTTCTACAATGGACAGAACATTTTTCAAGTTTCAGATCCCTCCTTCAAGCATGACAGCATTTGATATCATAAGCACATCAACATTTATCATATTACTCGACGTTCTTATTATCCCATTATATATTAAAGTGATGAAAAGATCTCCAAAACTTCCTACCGAGCTACAAAGTATTGGCATCGGACTCAGCATTACAATCATAACATTGATTGTTGCTGGTTTTGTAGAGCAGAGAAGATTAAGTTTTGCCAGCAATGATGGTAAAGAGATGAGTTCTTTGAGTATTTTTTGGTTGATACCACAATATATGCTTTTAGGAATAGCAGAAGCTTTTGTGTATGTAGCACAGATGAACTTTTTCACGTCACAAACTCCAGATGGTTTGGAAAGCTTGGGAATGGGGTTGTCTATGTTTTCATCCGCACTTGGTTGCTATGTTGGTAACATTTTTCTGACTGTGGTAAATAAAATTACATCAAGTGGTCAAGGACAACATGGTTGGGTTTCACCTAATCTAAATGACGGTCATTTAGATAGGTTTTTCTTCTTGACAGCATTTCTAATTGCTATTGACTTGATTGTGTATATTGTATGTGCCAAAAGATACAAGGGCATATAAAATGAGAAAAGAGAAGAAGCAACCAATTAGGAAGTTGTAGTTTGATTTGATAGAATGACAAGTGGAGTTGGATGCGTTTTAACAAATTTTTACAGAGCATCAAGAAGGATGTCCTGACTTTGGTGCCATAGTTTCTGATTGTAGACATTTCTTCTCTTTTGTTTTAACAACTCTAGTGTCAATTTATAAGAAGACATGTCAATGGGTAGCTCATCCCTTACCAAGGGTGGCCACATATTTAATTAGTTTTCAGCTTTTTATTGATACTTCTTATATTGATCATATTAACTGTTCCTGTTTCTCTCAGATCATTGGTCACTCACCCTATTGCAATCTCATTCTCCAACTCTTTGTTTTTTCCCCACAATATCAACAATATTTAGTGCAGATTTATCAGTCATGTAAAAACCAAATTTTGCCATCACTGTTCTAGTTCAACCTAACATTTCATTAAACTAGTTGTCCATGTGTCTGAAAAATTAATGCCAAGGTATGGCTTGAATTATATATAAATGACCAAGGTTTGACTAATGTAGGTAACACATACATAGAACACACATACAAATAGATATACATATGTACTTATGAATACAATATACATACTATGTTCATATTTGTTAATTGTAGCGGCTTAAAAATTCTAGGGTTGCTCGAAGAACAATGAAGTTGCCACTTAAGATATTATATTTGAGTAATAGTGTGAGAGTTGTCTACATGTTCGGAGGCAATACACAATATGTTGGAGGGGCAGTTCACATAGATATAGGGAGTTGTACCGTCTTGTAGCATAGATACAAATACAACTTTTTGTACTGAGTTGGAGGGTAATGTTTATACAGCTGGCTTGAGCTTTATTTTTGAGAGAGCAAAACACTCCAAGAAAACTTGCACGGATAAGAAAAAGCGTGGTTGTGTGATCATAACATCTTATGAGTTACCTTGTGCATGTATTCTTTCTATGAAGACCCATGACAAGAAGTGTGTGATCATAACATCTTATGAGTTACCTTGTGCATGTATTCTTTCTATGAAGACCCATGACAAAAAGTATATTCAATTGGGTGGGATAAACCCCCATTGAAAAAGGTTATGTATCAGTAAAGAAGAGGGTGAAGATTGTTTTTCTGACATGGATGAGTGAAATGAAATTTAGGAACGTCTCAAAAAATTCTCTTACAAATTGAAACTACATAGCAAGGAGGTTATTTATCAGCTAGCATTTCTAGTAATGACTAGTAATGACTTTGTTGACTCCGCCTCTAGAAAAGGTAGAAACCAAGAGCGCAAAAAAGGGTGAAACCTACACCAAATGATGCTTTGACTAGTCGAGTTCAATCTTTGTGGAAGATTGTTGATTCCTAATTTCTCGATATTCAAACATCACAAACAAATCCATCTTTTCATAAAAGGAAAGATTATTGATAATCTATCATGTTGTCCAATTCCAACGTCTACTCGGCTCTAAAGCCAGAGCCTATTGATCATATGCTAAAATTCATGAAAACATTTATTGAATTTTTTTTGGATGTTAAAGGCGATGGAAGGTGTGAATTTCGAACTATAGTTGAACACAAGGGTTTAATTGAAGAAATTCATTGTATTATTCGTAGAGCATTTATTAAAGACGTGAAAGATCATATGAGTGACTAAATTTGTGTATTTGGAATTAAGAAGCGTTTTAACCAAATCCTAAATGGTTTGTACCCTCTCCAAAATATTATTGGAATTGTATTTCAAAATAAGTGGTTAACATAGATTACATTATTTCAACTTGTTACAATAAAATAAATAAAAATATTGAATAGAACATCACATTGAACTACCCGTGTGTAATGCATGCGTCTTTTAGTTGGCAATTATAAATATGAAATGACACTTCAAACAATAATACCTGGATACTATGGTCATTCTTGGTTAATTAGCATCGTCTGGCACTCAAACAATACTTTTGAATTATTGGCATTAATACATAGGAAATGACTCACATCACTTAGCTGTGTGGAACGAGAAAATAAAAAAAAGTAGTAGTATTAAGTAATAAGTGGTCGTGGTTTGAAGACTCACTGACTGCATTAAAAAGAACCAGTAGGTAGGGATGTCATTAGGTATAAGCCTGTTGGACATCCAGAAAAATATCTACAATGGATAAGATAAATATTTGAATTTGTGTTTGAGTTTGGAAAAATATCTACGTAAATGTTATGTATAACTCATATTTCATAAAAAAAAAAAAAATTCTTTTATTACCTTGTTAAAAAACTTTTGCTTTCAATCCCTGATTCTTCCTCTTCTTTTCTATATTATTGATGATATTACTTTTCTAAATGTCAAGTGGTAAATATTAGAATATACGGTTAGTGTAAAGTTTATGAAATTTACAAATTTATATGTTTATATCTAGAAACTTTTGAAGTTATTAATGGTTGTCTTTTATGTTATGAAGAATTGTGAAAACTATATTCGTATTCATGATTTATGTTGAGAGAGATGTGTTCGATGACATAAATAATAAATTGATTATTCAATGATTTCATAAAATAAAATCAAGTAGTGGAAAATTGTAAAATATGTTTGTTATTTTGATTAGAATTTATTCTTTTGAATATATATTATTTATATAATATAAATAATATTTTGCCTTAAAAATGATGATTTTTGACGCCAAAAAATGTGTTTTAATTTAAAAAAAACATTGAAACAATAAATCATATTTTTTTTTGGGATAAAAAAATCACTTTGGCAAAAGAACATGGGTCAGCAGGACCATAATTGTAAGAAATGTGAAAATAGAGAGACAAAAATGTCTGACTTTTTTTATTTTTAAAAAGAGACTATTTTAGTAAGTTTTAAAAATAAGGGAAAAGTGCAATTAATTGTATTATTTTTATTGAATTTTCCCTAAAAAAAGAGTGTTAGAAAGAGATTGCTGAATACATTCCTTCTCACACGATTTGTAGCACTGACACTTTGATATATTAGCCATTGAACACTATATATACACACACTCAAATCATTACTTTCTGTCTTTATGAGCGTAGTTCAGTTGGTAGGGACATTGCGTATTATGCAGCTGTTGGGATTTGAACCCCGAACACTTCTCAAAATGTGTTTAAAATGTGTGAACTTCGGCCACTATACTACTTGACAAAAAAAAATCATGGCTTCCTCCAACTATATATACATACATCAGAATTAAATCATAACTAAATATAAACCATGGCGTCCTCCAACATATTCCAAATCTCATTCTCCCTTATCTTCTTAACATTCTTCCTCATCTTGTTACTCAACAAGGTGAACTCAACAGAATTCACTTCCTTTGCCATGCTAGATTTTTCCCAAGATCAAAACAATCTAATCTTCCAAGGTGATGCTTATATCACCTCATGAAACAAGTTACAACTCAACAAGGCAAAAAGTAGCTCAGTTGGTAGAGCACTGTACTCTGAACCTATCCACATTTGGGATAGCAAAACAGGAGAAGTTGCTCATTTTGATACATCCTTCAATTTCATCATAACTGCACCTGACACGGGCAATGTTGCAGACGGTTTTACTTTCTTTCTTGCACCAATAGATACTCAACCTCAAGATGGTGGAGGGTTTCTAGGGCTTTTTAACGACAAAAACTACAACAGATCTATTCAAACCGTTGCTGTTGAGTTTGACACATATTATAACTCTGATTGGGATCCAAGAGATCGACATAACGGAATCGATGTAAACTGTGTTAGGTCTACAACAACTAAACCATGGGTTTTCAGGGATGGTGGAGAGGGTATTGTTCTGATAAAATTTGATGCTTCTACTAATGTGTTAAGTGTTACTTTATTTACTGAGGATGGTATTTATACACTCAGTGATGTTGTGAATGTGAAGGATGTTCTTCCTGAGTGGATTAGGGTTGGCTTCTCAGCTGCCACTGGAGGTGACTTTTCAGTTCATGATATTCTTTCATGGCGATTTTCTTCTATTTTAAACCACCATTCAAGCTCCATGCAAGCTTCTGCATAG

Protein sequence

>MS.gene066599.t1

MALSLSTHFFLLKPHRCEQIGELCESDTQNQFPLFYLSIYLIALGSGVSDPALPTLGADQFDEEEPKEQRSKASIYGYFYVALNLGSLVAETILAYIETTGHWVLGFWICTSCAAVSFLVLLSGTLRYRHYKTFGNPFSKFSQLIVPFLKKMKFQIQSIGEDLYHDGIQGDDTRVRRIHHANGLRFFDRAGIVSDEATEMLLVMKRSPKLPTELQSIGIGLSITIITLIVAGFVEQRRLSFASNDGIAEAFVYVAQMNFFTSQTPDGLESLGMGLSMFSSALGCYVGNIFLTVVNKITSSGQGQHGWVSPNLNDGHLDRFFFLTAFLIAIDLIVYILQLNKAKSSSVGRALYSEPIHIWDSKTGEVAHFDTSFNFIITAPDTGNVADGFTFFLAPIDTQPQDGGGFLGLFNDKNYNRSIQTVAVEFDTYYNSDWDPRDRHNGIDVNCVRSTTTKPWVFRDGGEGIVLIKFDASTNVLSVTLFTEDGIYTLSDVVNVKDVLPEWIRVGFSAATGGDFSVHDILSWRFSSILNHHSSSMQASA