AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066643

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066643.t1 MTR_4g023030 96.950 623 19 0 1 623 1 623 0.0 1246
MS.gene066643.t1 MTR_3g026650 69.021 623 133 9 1 623 1 563 0.0 800
MS.gene066643.t1 MTR_3g026650 67.093 547 120 9 1 547 1 487 0.0 664
MS.gene066643.t1 MTR_3g026650 63.341 461 109 9 1 461 1 401 4.24e-177 508
MS.gene066643.t1 MTR_4g125460 36.977 430 251 8 198 616 58 478 2.53e-88 283
MS.gene066643.t1 MTR_4g125460 36.977 430 251 8 198 616 197 617 6.80e-87 283
MS.gene066643.t1 MTR_5g064620 34.286 490 267 12 164 616 40 511 9.15e-80 262
MS.gene066643.t1 MTR_3g096020 36.508 441 243 11 199 616 160 586 6.22e-79 261
MS.gene066643.t1 MTR_5g099010 36.427 431 255 9 196 619 84 502 4.39e-77 256
MS.gene066643.t1 MTR_4g100810 39.231 390 217 9 236 616 111 489 9.41e-77 254
MS.gene066643.t1 MTR_5g081690 34.967 449 271 10 179 616 72 510 1.97e-76 253
MS.gene066643.t1 MTR_3g115220 35.010 477 262 10 177 619 33 495 6.92e-76 253
MS.gene066643.t1 MTR_3g099480 36.501 463 241 13 184 616 98 537 3.01e-75 250
MS.gene066643.t1 MTR_4g082310 36.409 401 236 8 236 622 100 495 3.93e-75 249
MS.gene066643.t1 MTR_5g012770 37.907 430 234 11 202 622 95 500 1.08e-74 248
MS.gene066643.t1 MTR_4g019210 33.945 436 268 8 196 620 199 625 2.31e-73 248
MS.gene066643.t1 MTR_5g032100 34.725 455 246 10 201 616 59 501 3.35e-73 244
MS.gene066643.t1 MTR_5g042200 35.802 405 235 9 236 623 88 484 7.39e-71 237
MS.gene066643.t1 MTR_4g084570 33.784 444 258 10 201 622 124 553 8.51e-71 239
MS.gene066643.t1 MTR_4g110030 37.634 372 220 6 236 598 119 487 1.13e-70 238
MS.gene066643.t1 MTR_1g031590 33.547 468 270 12 175 617 96 547 1.74e-70 238
MS.gene066643.t1 MTR_6g016015 37.333 375 219 8 236 599 118 487 2.11e-70 236
MS.gene066643.t1 MTR_3g100310 33.698 457 250 10 200 616 72 515 2.57e-70 236
MS.gene066643.t1 MTR_2g059350 36.685 368 219 6 236 593 118 481 6.20e-69 233
MS.gene066643.t1 MTR_3g086570 32.883 444 261 12 199 616 115 547 5.84e-68 231
MS.gene066643.t1 MTR_5g059260 32.506 443 269 9 198 616 123 559 5.48e-65 224
MS.gene066643.t1 MTR_3g005340 33.333 396 248 6 236 616 150 544 5.28e-63 218
MS.gene066643.t1 MTR_1g102030 30.435 437 273 11 202 619 61 485 5.76e-58 203
MS.gene066643.t1 MTR_2g016470 29.325 474 264 16 199 616 156 614 8.58e-50 183
MS.gene066643.t1 MTR_4g023050 95.000 80 4 0 544 623 29 108 9.55e-50 168
MS.gene066643.t1 MTR_4g132590 30.917 469 262 17 199 616 35 492 1.34e-47 174
MS.gene066643.t1 MTR_4g132590 30.917 469 262 17 199 616 134 591 8.33e-47 174
MS.gene066643.t1 MTR_3g086570 31.077 325 190 10 199 498 115 430 1.46e-43 162
MS.gene066643.t1 MTR_1g101990 31.690 284 182 7 341 619 5 281 7.47e-43 157
MS.gene066643.t1 MTR_4g010990 27.318 399 234 13 236 616 78 438 4.12e-31 126
MS.gene066643.t1 MTR_7g017330 29.655 290 189 6 236 510 101 390 5.37e-30 125
MS.gene066643.t1 MTR_4g010990 26.684 386 227 13 249 616 2 349 1.98e-27 114
MS.gene066643.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 1.88e-26 114
MS.gene066643.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 2.63e-26 114
MS.gene066643.t1 MTR_1g088900 56.322 87 8 1 321 407 38 94 1.08e-22 98.2
MS.gene066643.t1 MTR_4g008200 24.737 380 219 12 194 561 14 338 3.19e-21 96.3
MS.gene066643.t1 MTR_2g038320 24.603 378 220 13 213 569 77 410 3.76e-21 96.7
MS.gene066643.t1 MTR_4g008200 24.352 386 213 12 194 561 65 389 4.60e-21 96.7
MS.gene066643.t1 MTR_1g007390 27.547 265 177 6 261 510 124 388 1.75e-20 96.3
MS.gene066643.t1 MTR_7g064210 31.100 209 129 4 339 535 19 224 2.05e-20 94.4
MS.gene066643.t1 MTR_1g101985 39.091 110 63 1 198 307 57 162 4.13e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066643.t1 AT2G44500 63.235 612 156 9 11 618 17 563 0.0 750
MS.gene066643.t1 AT3G07900 59.481 617 192 13 10 621 14 577 0.0 682
MS.gene066643.t1 AT5G63390 60.798 426 166 1 199 623 130 555 0.0 555
MS.gene066643.t1 AT2G44500 56.484 455 129 9 11 461 17 406 5.09e-163 473
MS.gene066643.t1 AT5G35570 37.358 439 243 11 198 619 220 643 3.96e-82 271
MS.gene066643.t1 AT1G76270 36.136 440 248 10 194 619 69 489 1.87e-79 262
MS.gene066643.t1 AT1G20550 36.279 430 261 10 194 619 72 492 6.29e-79 261
MS.gene066643.t1 AT4G24530 35.590 458 242 11 199 616 72 516 1.08e-77 256
MS.gene066643.t1 AT5G64600 36.199 442 245 11 199 617 88 515 1.01e-76 254
MS.gene066643.t1 AT3G54100 34.112 428 266 7 202 620 195 615 3.72e-76 255
MS.gene066643.t1 AT1G22460 35.845 438 244 10 202 616 134 557 6.51e-75 250
MS.gene066643.t1 AT5G01100 34.101 434 266 8 202 623 190 615 6.98e-74 249
MS.gene066643.t1 AT5G65470 33.913 460 251 10 199 617 56 503 2.39e-73 244
MS.gene066643.t1 AT2G03280 38.070 373 215 7 237 598 92 459 3.65e-73 243
MS.gene066643.t1 AT2G37980 33.103 435 261 9 202 620 199 619 3.98e-73 247
MS.gene066643.t1 AT4G38390 38.196 377 204 7 235 596 125 487 3.40e-72 243
MS.gene066643.t1 AT1G14020 35.930 398 234 8 237 619 95 486 9.15e-70 234
MS.gene066643.t1 AT5G15740 36.193 373 224 6 236 598 109 477 2.84e-69 233
MS.gene066643.t1 AT1G14970 33.333 438 256 9 201 616 9 432 3.17e-69 231
MS.gene066643.t1 AT1G14970 32.479 468 279 10 172 616 99 552 2.20e-67 230
MS.gene066643.t1 AT2G03280 35.500 400 215 8 237 598 92 486 2.38e-67 228
MS.gene066643.t1 AT4G16650 40.360 389 216 11 236 617 125 504 4.65e-67 229
MS.gene066643.t1 AT4G16650 40.360 389 216 11 236 617 125 504 4.65e-67 229
MS.gene066643.t1 AT1G14970 33.738 412 245 7 226 616 2 406 1.17e-66 224
MS.gene066643.t1 AT3G02250 34.828 379 221 6 236 598 109 477 1.30e-65 224
MS.gene066643.t1 AT3G26370 33.668 398 244 8 236 616 161 555 3.26e-65 224
MS.gene066643.t1 AT1G04910 30.979 439 276 11 191 616 50 474 4.58e-65 223
MS.gene066643.t1 AT1G35510 32.118 439 262 9 202 618 134 558 1.00e-62 218
MS.gene066643.t1 AT1G38065 32.850 414 254 10 224 616 18 428 1.07e-62 214
MS.gene066643.t1 AT1G38065 33.085 402 246 9 236 616 39 438 1.44e-62 214
MS.gene066643.t1 AT2G01480 32.723 437 258 10 201 615 128 550 4.82e-62 216
MS.gene066643.t1 AT1G38131 32.850 414 254 10 224 616 147 557 1.39e-61 215
MS.gene066643.t1 AT2G01480 33.671 395 231 7 201 575 128 511 3.95e-60 209
MS.gene066643.t1 AT1G62330 29.193 483 259 15 198 618 190 651 1.19e-54 197
MS.gene066643.t1 AT1G11990 30.737 475 288 16 167 617 132 589 8.84e-54 194
MS.gene066643.t1 AT1G11990 29.106 481 267 15 198 617 144 611 1.05e-47 177
MS.gene066643.t1 AT1G29200 32.613 463 235 18 212 616 50 493 2.86e-47 174
MS.gene066643.t1 AT1G29200 32.613 463 235 18 212 616 50 493 2.86e-47 174
MS.gene066643.t1 AT1G29200 32.613 463 235 18 212 616 166 609 1.24e-46 174
MS.gene066643.t1 AT1G29200 33.333 432 222 16 238 616 1 419 8.97e-46 167
MS.gene066643.t1 AT3G30300 32.759 290 180 6 236 510 103 392 5.35e-35 141
MS.gene066643.t1 AT3G30300 32.759 290 180 6 236 510 103 392 5.35e-35 141
MS.gene066643.t1 AT1G52630 24.480 433 280 13 195 616 39 435 9.89e-30 122
MS.gene066643.t1 AT3G26370 32.967 182 108 4 236 404 161 341 8.86e-29 119
MS.gene066643.t1 AT3G03810 30.375 293 183 7 236 510 92 381 6.72e-27 116
MS.gene066643.t1 AT3G03810 30.375 293 183 7 236 510 92 381 6.72e-27 116
MS.gene066643.t1 AT1G52630 24.802 379 241 12 249 616 2 347 2.22e-25 108

Find 140 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTGGTTGTTTC+TGG 0.073685 4.2:-79829932 MS.gene066643:CDS
ACCAAACCCATTTGAGATTA+AGG 0.186427 4.2:+79830339 None:intergenic
GCCATCTCATGGAGGAAATA+TGG 0.218103 4.2:-79827929 MS.gene066643:CDS
GTTGTTATTGCTAGGATTCT+TGG 0.223923 4.2:-79829872 MS.gene066643:CDS
ATGACATTAACTTGCAAAAT+AGG 0.227744 4.2:+79829833 None:intergenic
TCTTCACCACCTTAACAAAA+TGG 0.248210 4.2:-79830489 MS.gene066643:CDS
TATAGGTTGCTTAAAGCTTT+AGG 0.261962 4.2:-79828156 MS.gene066643:intron
AGAGAAGCTTTCTTCGAAAA+TGG 0.262334 4.2:+79828000 None:intergenic
GAGGGAAGAATTCAGAAAAT+AGG 0.283536 4.2:+79827370 None:intergenic
GACCAGCCATCTTTCTTTCA+TGG 0.284935 4.2:+79828583 None:intergenic
GTTTCCACATCTTTATAATA+AGG 0.294769 4.2:-79828052 MS.gene066643:CDS
TTTGCAAGTTAATGTCATTT+GGG 0.301248 4.2:-79829828 MS.gene066643:CDS
AAAAGATGCAAGAATTTATT+GGG 0.322642 4.2:-79828127 MS.gene066643:CDS
AAATATGGGCCATGCGCTTC+AGG 0.329180 4.2:-79827914 MS.gene066643:intron
GAAAAGCCCTTAATCTCAAA+TGG 0.333986 4.2:-79830346 MS.gene066643:CDS
CCAAACCCATTTGAGATTAA+GGG 0.341142 4.2:+79830340 None:intergenic
CAAAACCCTTCAATCTCAAA+TGG 0.341182 4.2:-79830229 MS.gene066643:CDS
CCCTTAATCTCAAATGGGTT+TGG 0.349127 4.2:-79830340 MS.gene066643:CDS
CTCAGAAACTATGTAATCTA+TGG 0.350072 4.2:+79827969 None:intergenic
TCAAATTCACTTCCTTTGAT+TGG 0.352095 4.2:-79830136 MS.gene066643:CDS
TGGAAGCAACCAGATGGGTT+AGG 0.362833 4.2:-79830028 MS.gene066643:CDS
TGACTTGGAGGAGGAAGAAA+AGG 0.368313 4.2:-79830077 MS.gene066643:CDS
TAGGATTCTTGGTGCTGCTT+TGG 0.371467 4.2:-79829861 MS.gene066643:CDS
ATGCTGTTAAACTCTGCTTC+AGG 0.374694 4.2:+79827347 None:intergenic
AGGGAAGAATTCAGAAAATA+GGG 0.381917 4.2:+79827371 None:intergenic
TTGAATAGAAGAAGATGAAA+TGG 0.388256 4.2:+79830570 None:intergenic
TGGTTCAAAATCTGAACTTA+AGG 0.388422 4.2:-79830320 MS.gene066643:CDS
TTCGAAGAAAGCTTCTCTAA+TGG 0.388722 4.2:-79827995 MS.gene066643:CDS
GTTAGGGTATAAGCCTTGTT+TGG 0.390330 4.2:-79830011 MS.gene066643:CDS
GAATTGCATCAGAATTCATT+AGG 0.395674 4.2:-79827317 MS.gene066643:CDS
TTGCACATGCACTCCGGAAC+AGG 0.396099 4.2:+79827245 None:intergenic
GGATTGTGAAGAATAGAAAA+AGG 0.396440 4.2:-79829955 MS.gene066643:CDS
TGTGGAAACTCATGGATTAA+TGG 0.400222 4.2:+79828066 None:intergenic
GAGTTGAAAAGAGTGAGTTT+TGG 0.403781 4.2:-79830048 MS.gene066643:CDS
TTGTTTCCAAGTTCTTGAAC+TGG 0.412948 4.2:+79828783 None:intergenic
ACCTTGCTAGTCCTGAGTTC+CGG 0.418631 4.2:+79827290 None:intergenic
GCGAAACCAGTTCAAGAACT+TGG 0.420208 4.2:-79828789 MS.gene066643:CDS
GTGCGGACTGGTTGTCTACC+TGG 0.424088 4.2:-79828690 MS.gene066643:CDS
CGAAACCACCCGCACATCAT+TGG 0.433723 4.2:+79829355 None:intergenic
GGAAGCAACCAGATGGGTTA+GGG 0.436626 4.2:-79830027 MS.gene066643:CDS
TTTGTCATCAAGTGTGTTGA+TGG 0.436659 4.2:+79829326 None:intergenic
TCAAACCCATTTGAGATTGA+AGG 0.439951 4.2:+79830223 None:intergenic
ATGCAAGAATTTATTGGGCC+GGG 0.443277 4.2:-79828122 MS.gene066643:CDS
GTATTTCTTGTGTCCTGCAT+AGG 0.451809 4.2:+79827421 None:intergenic
GGCTTATACCCTAACCCATC+TGG 0.457469 4.2:+79830019 None:intergenic
TGTCACGAAGTATCCTGTTC+CGG 0.458292 4.2:-79827258 MS.gene066643:CDS
GTTATTCCTTCCAGGGTCAC+AGG 0.458415 4.2:-79827445 MS.gene066643:intron
GGTTCCTAGTGGAGTTGACT+TGG 0.467672 4.2:-79830092 MS.gene066643:CDS
TATAAAGATGTGGAAACTCA+TGG 0.469538 4.2:+79828058 None:intergenic
AAGGAAGATCTTGCATTGCC+TGG 0.471250 4.2:-79828033 MS.gene066643:CDS
GGAGGAGGAAGAAAAGGGTG+AGG 0.471319 4.2:-79830071 MS.gene066643:CDS
TCCACCCAATGGTTGTCCCC+CGG 0.472197 4.2:+79828104 None:intergenic
AAAACCCTTCAATCTCAAAT+GGG 0.474207 4.2:-79830228 MS.gene066643:CDS
TGCTGTTAAACTCTGCTTCA+GGG 0.476049 4.2:+79827348 None:intergenic
CCATCTCATGGAGGAAATAT+GGG 0.480168 4.2:-79827928 MS.gene066643:CDS
TAGCGCGAACGGATCCAACT+TGG 0.483932 4.2:+79829269 None:intergenic
AAAAGCCCTTAATCTCAAAT+GGG 0.485351 4.2:-79830345 MS.gene066643:CDS
CCTTGCTCTTCATCTAAGAA+TGG 0.490124 4.2:-79828727 MS.gene066643:CDS
CTTATGGTGGTTGTTTCTGG+AGG 0.490684 4.2:-79829929 MS.gene066643:CDS
GATGCAAGAATTTATTGGGC+CGG 0.492621 4.2:-79828123 MS.gene066643:CDS
CAAACCCATTTGAGATTGAA+GGG 0.494557 4.2:+79830224 None:intergenic
GATCTTCCTTTCAAGAGCTC+CGG 0.494862 4.2:+79828618 None:intergenic
CTCTTCATGTCACTCCAAGT+TGG 0.504620 4.2:-79829283 MS.gene066643:CDS
TGCAAGAATTTATTGGGCCG+GGG 0.505342 4.2:-79828121 MS.gene066643:CDS
GAAAATGGTTCTAGTTCTCC+AGG 0.505643 4.2:+79828015 None:intergenic
AAGGATGTGTGGGTGCGGAC+TGG 0.506559 4.2:-79828702 MS.gene066643:CDS
AACTCAGGACTAGCAAGGTC+GGG 0.506943 4.2:-79827286 MS.gene066643:CDS
AGCCTTGTTTGGATTTCAGT+AGG 0.508226 4.2:-79830000 MS.gene066643:CDS
CCTTTGCAGTTCAACAGAGA+AGG 0.513180 4.2:-79828996 MS.gene066643:intron
TCTTTGTCCCTTGACTGCCA+TGG 0.516067 4.2:-79828562 MS.gene066643:CDS
TCCTAGTGGAGTTGACTTGG+AGG 0.524121 4.2:-79830089 MS.gene066643:CDS
ATTATAGAAGAGAAAGTGAT+GGG 0.526617 4.2:-79829976 MS.gene066643:CDS
TCTTCATCTAAGAATGGAAA+AGG 0.529212 4.2:-79828721 MS.gene066643:CDS
GACTTGGAGGAGGAAGAAAA+GGG 0.531687 4.2:-79830076 MS.gene066643:CDS
ATGTCATTTGGGGAGATGAA+AGG 0.532142 4.2:-79829817 MS.gene066643:intron
GCCTTGTTTGGATTTCAGTA+GGG 0.532530 4.2:-79829999 MS.gene066643:CDS
GGTAACATCCATGGCAGTCA+AGG 0.535475 4.2:+79828554 None:intergenic
TCTTAGATGAAGAGCAAGGT+AGG 0.537764 4.2:+79828731 None:intergenic
AACATTACATTACCAATCAA+AGG 0.538428 4.2:+79830124 None:intergenic
GAATGAGAGGAGAGAACGAC+CGG 0.540471 4.2:-79828637 MS.gene066643:CDS
CGAAGCTTCTGAAGATCTGA+AGG 0.542834 4.2:+79828927 None:intergenic
TATTTACCTGGTAACATCCA+TGG 0.544732 4.2:+79828545 None:intergenic
CAGGGTCACAGGGCCTATGC+AGG 0.546366 4.2:-79827434 MS.gene066643:CDS
TCAGAATTCATTAGGACAGC+CGG 0.547453 4.2:-79827309 MS.gene066643:CDS
TAATGTAATGTTGCAAGCAA+AGG 0.548895 4.2:-79830113 MS.gene066643:CDS
CATTAGGACAGCCGGAACTC+AGG 0.555136 4.2:-79827301 MS.gene066643:CDS
AGAGGAGGACTTCCCTCCAC+TGG 0.555343 4.2:+79829302 None:intergenic
TTGATGCTGTTGTTATTGCT+AGG 0.555554 4.2:-79829880 MS.gene066643:CDS
ACGAGATTGTAAAGAATGAG+AGG 0.556539 4.2:-79828650 MS.gene066643:CDS
GAACGACCGGAGCTCTTGAA+AGG 0.557470 4.2:-79828624 MS.gene066643:CDS
GATTATAGAAGAGAAAGTGA+TGG 0.557924 4.2:-79829977 MS.gene066643:CDS
TTTACAATCTCGTCAAACTC+AGG 0.561693 4.2:+79828660 None:intergenic
GAGGAGGAAGAAAAGGGTGA+GGG 0.561708 4.2:-79830070 MS.gene066643:CDS
TACCATGAAAGAAAGATGGC+TGG 0.562902 4.2:-79828585 MS.gene066643:CDS
CCCATATTTCCTCCATGAGA+TGG 0.566943 4.2:+79827928 None:intergenic
ATGGAATTGATTATTTGTGA+AGG 0.568945 4.2:+79830589 None:intergenic
TTATGGTGGTTGTTTCTGGA+GGG 0.569275 4.2:-79829928 MS.gene066643:CDS
TCTTCAGAAGCTTCGATGCA+AGG 0.571820 4.2:-79828919 MS.gene066643:intron
CAACTTGGAGTGACATGAAG+AGG 0.572279 4.2:+79829284 None:intergenic
TTCAACATTTGTAGCACATG+GGG 0.574252 4.2:+79830366 None:intergenic
TAGTGGAGTTGACTTGGAGG+AGG 0.574980 4.2:-79830086 MS.gene066643:CDS
AAAAGAAGAACAATCTTGAA+GGG 0.576810 4.2:+79830461 None:intergenic
GACTTACCATGAAAGAAAGA+TGG 0.579868 4.2:-79828589 MS.gene066643:CDS
GAACTCAGGACTAGCAAGGT+CGG 0.582708 4.2:-79827287 MS.gene066643:CDS
TTATTCCTTCCAGGGTCACA+GGG 0.583835 4.2:-79827444 MS.gene066643:intron
TTTGATTCAAAATCTGAACT+TGG 0.584212 4.2:-79830205 MS.gene066643:CDS
TGTTCTTGCCAATGATGTGC+GGG 0.584306 4.2:-79829363 MS.gene066643:CDS
CTTGGATTCAAGGCTATCAA+AGG 0.589401 4.2:-79828955 MS.gene066643:CDS
TCTTCCTTATTATAAAGATG+TGG 0.591443 4.2:+79828048 None:intergenic
TGACTGCCATGGATGTTACC+AGG 0.594053 4.2:-79828551 MS.gene066643:intron
TTTCAACATTTGTAGCACAT+GGG 0.594469 4.2:+79830365 None:intergenic
TCCTCCAAGTCAACTCCACT+AGG 0.596678 4.2:+79830088 None:intergenic
TGGAAAAGGATGTGTGGGTG+CGG 0.600501 4.2:-79828707 MS.gene066643:CDS
GCAAGAATTTATTGGGCCGG+GGG 0.602690 4.2:-79828120 MS.gene066643:CDS
TTGCAAGTTAATGTCATTTG+GGG 0.605474 4.2:-79829827 MS.gene066643:CDS
GTTAAACTCTGCTTCAGGGA+GGG 0.608437 4.2:+79827352 None:intergenic
TCAGGACTAGCAAGGTCGGG+AGG 0.608590 4.2:-79827283 MS.gene066643:CDS
CTTAGATGAAGAGCAAGGTA+GGG 0.612955 4.2:+79828732 None:intergenic
CCATTCTTAGATGAAGAGCA+AGG 0.618532 4.2:+79828727 None:intergenic
GTAACATCCATGGCAGTCAA+GGG 0.621567 4.2:+79828555 None:intergenic
CTTGATGACAAAGCCAGTGG+AGG 0.623445 4.2:-79829315 MS.gene066643:CDS
GCTGAGAGAATGCAAAGCAA+AGG 0.624230 4.2:-79828756 MS.gene066643:CDS
TTGATGACAAAGCCAGTGGA+GGG 0.626586 4.2:-79829314 MS.gene066643:CDS
CAGGACTAGCAAGGTCGGGA+GGG 0.628531 4.2:-79827282 MS.gene066643:CDS
TGTTTCAATGAATAAAGATG+AGG 0.632505 4.2:-79830179 MS.gene066643:CDS
TTTGGTAAAATCTGAACTTG+GGG 0.642353 4.2:-79830257 MS.gene066643:CDS
GAGTCGTTGCACATGCACTC+CGG 0.647476 4.2:+79827239 None:intergenic
CTTGGAGTGACATGAAGAGG+AGG 0.650220 4.2:+79829287 None:intergenic
AAGAATGGAAAAGGATGTGT+GGG 0.654741 4.2:-79828712 MS.gene066643:CDS
TCCCTACTGAAATCCAAACA+AGG 0.655365 4.2:+79829998 None:intergenic
TTGCAAGCAAAGGTTCCTAG+TGG 0.655758 4.2:-79830103 MS.gene066643:CDS
TAAGAATGGAAAAGGATGTG+TGG 0.664578 4.2:-79828713 MS.gene066643:CDS
GCCGGAACTCAGGACTAGCA+AGG 0.665088 4.2:-79827291 MS.gene066643:CDS
CTGGAGGGATGAATCAGCAG+AGG 0.666148 4.2:-79829913 MS.gene066643:CDS
ATAGTCATACCTGAAGCGCA+TGG 0.668438 4.2:+79827905 None:intergenic
TCAAACTCAGGACTTAGACC+AGG 0.670921 4.2:+79828672 None:intergenic
ATGTTCTTGCCAATGATGTG+CGG 0.671242 4.2:-79829364 MS.gene066643:CDS
ACACTTGATGACAAAGCCAG+TGG 0.673399 4.2:-79829318 MS.gene066643:CDS
TCTTGCCAATGATGTGCGGG+TGG 0.696204 4.2:-79829360 MS.gene066643:CDS
TGTTAAACTCTGCTTCAGGG+AGG 0.699216 4.2:+79827351 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGAGATTTTTAA+TGG - chr4.2:79827574-79827593 MS.gene066643:intron 10.0%
!!! TTTATTTGTTTTTGATTTAT+AGG - chr4.2:79829687-79829706 MS.gene066643:intron 10.0%
!! TTATTAAATATGAAGACTAA+AGG + chr4.2:79830161-79830180 None:intergenic 15.0%
!! AAAAAGATGCAAGAATTTAT+TGG - chr4.2:79829732-79829751 MS.gene066643:intron 20.0%
!! AAAAGATGCAAGAATTTATT+GGG - chr4.2:79829733-79829752 MS.gene066643:intron 20.0%
!! AATCAATGAAGATTTATGTA+AGG - chr4.2:79828120-79828139 MS.gene066643:CDS 20.0%
!! AGAAGAATTTAATATTTACC+TGG + chr4.2:79829330-79829349 None:intergenic 20.0%
!! ATATTTAGCTGTTACAAATT+TGG - chr4.2:79828230-79828249 MS.gene066643:intron 20.0%
!! GCTAAATATATATCCAAATT+TGG + chr4.2:79828219-79828238 None:intergenic 20.0%
!! TTACACTTATGAAACTATTT+GGG - chr4.2:79829403-79829422 MS.gene066643:CDS 20.0%
!! TTCTGTGGAAATAAAAATTT+GGG - chr4.2:79828788-79828807 MS.gene066643:CDS 20.0%
!! TTTACACTTATGAAACTATT+TGG - chr4.2:79829402-79829421 MS.gene066643:CDS 20.0%
!!! AAACATATTTTCTCCATAAA+TGG - chr4.2:79830269-79830288 MS.gene066643:CDS 20.0%
!!! AAATTCTTCTACTTTTGTAT+AGG - chr4.2:79829340-79829359 MS.gene066643:CDS 20.0%
!!! AGGTTTAGTTATTACAATTT+TGG - chr4.2:79828140-79828159 MS.gene066643:CDS 20.0%
!!! ATGATACAATCAATTCTTTT+AGG - chr4.2:79830214-79830233 MS.gene066643:CDS 20.0%
!!! ATTTTTGTTGAAGATTTCTT+TGG + chr4.2:79827337-79827356 None:intergenic 20.0%
!!! TGATACAATCAATTCTTTTA+GGG - chr4.2:79830215-79830234 MS.gene066643:CDS 20.0%
!!! TGTGCTTTTAGTAATTAAAA+AGG - chr4.2:79830187-79830206 MS.gene066643:CDS 20.0%
!!! TTATGGAGAAAATATGTTTT+TGG + chr4.2:79830268-79830287 None:intergenic 20.0%
!!! TTTATGATGTTTTTCCTTAT+TGG - chr4.2:79828416-79828435 MS.gene066643:intron 20.0%
! AAAAAGAAGAACAATCTTGA+AGG + chr4.2:79827403-79827422 None:intergenic 25.0%
! AAAAGAAGAACAATCTTGAA+GGG + chr4.2:79827402-79827421 None:intergenic 25.0%
! AAACAAAACCAAAACCAATA+AGG + chr4.2:79828433-79828452 None:intergenic 25.0%
! AACATTACATTACCAATCAA+AGG + chr4.2:79827739-79827758 None:intergenic 25.0%
! AATTAAGATAGTCACATTGA+TGG - chr4.2:79830301-79830320 MS.gene066643:CDS 25.0%
! AATTGCATGATTACCATTTA+TGG + chr4.2:79830285-79830304 None:intergenic 25.0%
! ATGGAATTGATTATTTGTGA+AGG + chr4.2:79827274-79827293 None:intergenic 25.0%
! ATTATAGAAGAGAAAGTGAT+GGG - chr4.2:79827884-79827903 MS.gene066643:intron 25.0%
! ATTGTTTGAAAGACCAAATT+TGG - chr4.2:79828203-79828222 MS.gene066643:intron 25.0%
! CTTCTGTGGAAATAAAAATT+TGG - chr4.2:79828787-79828806 MS.gene066643:CDS 25.0%
! GAATAGTATATATGAATGCA+TGG + chr4.2:79830075-79830094 None:intergenic 25.0%
! GTAATCATGCTTACATATAT+TGG - chr4.2:79830049-79830068 MS.gene066643:CDS 25.0%
! GTTTCCACATCTTTATAATA+AGG - chr4.2:79829808-79829827 MS.gene066643:intron 25.0%
! TCTTCCTTATTATAAAGATG+TGG + chr4.2:79829815-79829834 None:intergenic 25.0%
! TGTTTCAATGAATAAAGATG+AGG - chr4.2:79827681-79827700 MS.gene066643:intron 25.0%
! TTGAATAGAAGAAGATGAAA+TGG + chr4.2:79827293-79827312 None:intergenic 25.0%
! TTTACTATTCTTCTTGTTCA+TGG - chr4.2:79828071-79828090 MS.gene066643:CDS 25.0%
! TTTGATTCAAAATCTGAACT+TGG - chr4.2:79827655-79827674 MS.gene066643:intron 25.0%
!! AAGGTGAGTACAAATTTTAT+AGG + chr4.2:79830142-79830161 None:intergenic 25.0%
!! AATCTTTTTGTTCTTGATAC+TGG - chr4.2:79828322-79828341 MS.gene066643:intron 25.0%
!! ACAAATTTTATAGGGACTAA+AGG + chr4.2:79830133-79830152 None:intergenic 25.0%
!! AGGTGAGTACAAATTTTATA+GGG + chr4.2:79830141-79830160 None:intergenic 25.0%
!! TTTGCAAGTTAATGTCATTT+GGG - chr4.2:79828032-79828051 MS.gene066643:CDS 25.0%
!! TTTTGCAAGTTAATGTCATT+TGG - chr4.2:79828031-79828050 MS.gene066643:CDS 25.0%
!! TTTTGGTAAAATCTGAACTT+GGG - chr4.2:79827602-79827621 MS.gene066643:intron 25.0%
!! TTTTTCACACTCTTTTTTCT+TGG - chr4.2:79827418-79827437 MS.gene066643:CDS 25.0%
!!! ATGACATTAACTTGCAAAAT+AGG + chr4.2:79828030-79828049 None:intergenic 25.0%
!!! ATGTTTTTCCTTATTGGTTT+TGG - chr4.2:79828422-79828441 MS.gene066643:intron 25.0%
!!! GAATAGAAAAAGGTATCTTA+TGG - chr4.2:79827915-79827934 MS.gene066643:CDS 25.0%
!!! GATTTTTAATGGTGATGTTT+TGG - chr4.2:79827585-79827604 MS.gene066643:intron 25.0%
!!! TCTTATGTTTTAGCATCTTT+AGG - chr4.2:79828649-79828668 MS.gene066643:CDS 25.0%
AAAACCCTTCAATCTCAAAT+GGG - chr4.2:79827632-79827651 MS.gene066643:intron 30.0%
AAAAGCCCTTAATCTCAAAT+GGG - chr4.2:79827515-79827534 MS.gene066643:intron 30.0%
AACAAGATCAATCTATGCTT+AGG + chr4.2:79828178-79828197 None:intergenic 30.0%
AATAAGCTGGAAACAGTTTA+TGG + chr4.2:79829455-79829474 None:intergenic 30.0%
AGGGAAGAATTCAGAAAATA+GGG + chr4.2:79830492-79830511 None:intergenic 30.0%
ATCTTCATCTGTGTAACAAT+CGG - chr4.2:79828984-79829003 MS.gene066643:CDS 30.0%
CTCAGAAACTATGTAATCTA+TGG + chr4.2:79829894-79829913 None:intergenic 30.0%
GAATTGCATCAGAATTCATT+AGG - chr4.2:79830543-79830562 MS.gene066643:CDS 30.0%
GATTATAGAAGAGAAAGTGA+TGG - chr4.2:79827883-79827902 MS.gene066643:intron 30.0%
GCAAAAGTAGACAAAAACTT+AGG + chr4.2:79828682-79828701 None:intergenic 30.0%
GGATTGTGAAGAATAGAAAA+AGG - chr4.2:79827905-79827924 MS.gene066643:intron 30.0%
TAAAAGCAACCTGAAAACAT+AGG + chr4.2:79829037-79829056 None:intergenic 30.0%
TAATGTAATGTTGCAAGCAA+AGG - chr4.2:79827747-79827766 MS.gene066643:intron 30.0%
TATAAAGATGTGGAAACTCA+TGG + chr4.2:79829805-79829824 None:intergenic 30.0%
TATAGGTTGCTTAAAGCTTT+AGG - chr4.2:79829704-79829723 MS.gene066643:intron 30.0%
TCAAATTCACTTCCTTTGAT+TGG - chr4.2:79827724-79827743 MS.gene066643:intron 30.0%
TCTTCATCTAAGAATGGAAA+AGG - chr4.2:79829139-79829158 MS.gene066643:intron 30.0%
TCTTCTAGCATTCACAAATA+AGG + chr4.2:79828383-79828402 None:intergenic 30.0%
TGGTTCAAAATCTGAACTTA+AGG - chr4.2:79827540-79827559 MS.gene066643:intron 30.0%
TGTTGAACTAGAGTTTAACA+TGG - chr4.2:79828831-79828850 MS.gene066643:intron 30.0%
TGTTTATCCAAAGATCCTAT+AGG - chr4.2:79829618-79829637 MS.gene066643:intron 30.0%
TTTCAACATTTGTAGCACAT+GGG + chr4.2:79827498-79827517 None:intergenic 30.0%
TTTGGTAAAATCTGAACTTG+GGG - chr4.2:79827603-79827622 MS.gene066643:intron 30.0%
! AATAAAAGCTTTGACTTCTG+TGG - chr4.2:79828773-79828792 MS.gene066643:CDS 30.0%
! ACTGATTTCATAAGCTATCT+TGG + chr4.2:79829490-79829509 None:intergenic 30.0%
! AGGGTTTTTCCATTTTGTTA+AGG + chr4.2:79827383-79827402 None:intergenic 30.0%
! CTCTTATTCGAGTTTATTAC+TGG - chr4.2:79829377-79829396 MS.gene066643:CDS 30.0%
! GTTTTTCCATTTTGTTAAGG+TGG + chr4.2:79827380-79827399 None:intergenic 30.0%
! TTGATGAGTCGTGATTTTAT+AGG - chr4.2:79830241-79830260 MS.gene066643:CDS 30.0%
! TTGCAAGTTAATGTCATTTG+GGG - chr4.2:79828033-79828052 MS.gene066643:CDS 30.0%
! TTTTCAACATTTGTAGCACA+TGG + chr4.2:79827499-79827518 None:intergenic 30.0%
!! ATGCATTTGTCTTTTGTTTG+GGG + chr4.2:79830469-79830488 None:intergenic 30.0%
!! GATGCATTTGTCTTTTGTTT+GGG + chr4.2:79830470-79830489 None:intergenic 30.0%
!! GTTTTGGTAAAATCTGAACT+TGG - chr4.2:79827601-79827620 MS.gene066643:intron 30.0%
!! TAGAAAAAGGTATCTTATGG+TGG - chr4.2:79827918-79827937 MS.gene066643:CDS 30.0%
!!! ATGAGGTTTTGAATGATGTT+AGG - chr4.2:79827698-79827717 MS.gene066643:intron 30.0%
!!! GGTTTTGCTTTGTTTATGAT+TGG - chr4.2:79827442-79827461 MS.gene066643:intron 30.0%
AAACTATTTGGGAGAGCTTA+TGG - chr4.2:79829414-79829433 MS.gene066643:intron 35.0%
AAGAATGGAAAAGGATGTGT+GGG - chr4.2:79829148-79829167 MS.gene066643:intron 35.0%
ACCTAAATGTTATTCCTTCC+AGG - chr4.2:79830407-79830426 MS.gene066643:CDS 35.0%
ACGAGATTGTAAAGAATGAG+AGG - chr4.2:79829210-79829229 MS.gene066643:intron 35.0%
AGAGAAGCTTTCTTCGAAAA+TGG + chr4.2:79829863-79829882 None:intergenic 35.0%
CAAAACCCTTCAATCTCAAA+TGG - chr4.2:79827631-79827650 MS.gene066643:intron 35.0%
CCTAAATGTTATTCCTTCCA+GGG - chr4.2:79830408-79830427 MS.gene066643:CDS 35.0%
CTCTTGAGAAAGAACAACTT+TGG - chr4.2:79828258-79828277 MS.gene066643:intron 35.0%
GAAAAGCCCTTAATCTCAAA+TGG - chr4.2:79827514-79827533 MS.gene066643:intron 35.0%
GACTTACCATGAAAGAAAGA+TGG - chr4.2:79829271-79829290 MS.gene066643:CDS 35.0%
GAGGGAAGAATTCAGAAAAT+AGG + chr4.2:79830493-79830512 None:intergenic 35.0%
TAAGAATGGAAAAGGATGTG+TGG - chr4.2:79829147-79829166 MS.gene066643:intron 35.0%
TATTTACCTGGTAACATCCA+TGG + chr4.2:79829318-79829337 None:intergenic 35.0%
TCTTCACCACCTTAACAAAA+TGG - chr4.2:79827371-79827390 MS.gene066643:CDS 35.0%
TTATAGGGACTAAAGGTGAA+AGG + chr4.2:79830126-79830145 None:intergenic 35.0%
TTCAACATTTGTAGCACATG+GGG + chr4.2:79827497-79827516 None:intergenic 35.0%
TTCGAAGAAAGCTTCTCTAA+TGG - chr4.2:79829865-79829884 MS.gene066643:CDS 35.0%
TTGTTTCCAAGTTCTTGAAC+TGG + chr4.2:79829080-79829099 None:intergenic 35.0%
TTTACAATCTCGTCAAACTC+AGG + chr4.2:79829203-79829222 None:intergenic 35.0%
TTTGTCATCAAGTGTGTTGA+TGG + chr4.2:79828537-79828556 None:intergenic 35.0%
! ACCAAACCCATTTGAGATTA+AGG + chr4.2:79827524-79827543 None:intergenic 35.0%
! CAAACCCATTTGAGATTGAA+GGG + chr4.2:79827639-79827658 None:intergenic 35.0%
! CCAAACCCATTTGAGATTAA+GGG + chr4.2:79827523-79827542 None:intergenic 35.0%
! CTTTTGTATAGGAGTCTGTT+TGG - chr4.2:79829351-79829370 MS.gene066643:CDS 35.0%
! GAGTTGAAAAGAGTGAGTTT+TGG - chr4.2:79827812-79827831 MS.gene066643:intron 35.0%
! GGATGCATTTGTCTTTTGTT+TGG + chr4.2:79830471-79830490 None:intergenic 35.0%
! GTTGTTATTGCTAGGATTCT+TGG - chr4.2:79827988-79828007 MS.gene066643:CDS 35.0%
! TATCTTATGGTGGTTGTTTC+TGG - chr4.2:79827928-79827947 MS.gene066643:CDS 35.0%
! TCAAACCCATTTGAGATTGA+AGG + chr4.2:79827640-79827659 None:intergenic 35.0%
! TGTGGAAACTCATGGATTAA+TGG + chr4.2:79829797-79829816 None:intergenic 35.0%
! TTGATGCTGTTGTTATTGCT+AGG - chr4.2:79827980-79827999 MS.gene066643:CDS 35.0%
!!! GCACATGGGGTTTTAAAAAA+CGG + chr4.2:79827484-79827503 None:intergenic 35.0%
!!! TTCACACTCTTTTTTCTTGG+TGG - chr4.2:79827421-79827440 MS.gene066643:CDS 35.0%
AAGCTATCTTGGAGATCTTG+TGG + chr4.2:79829479-79829498 None:intergenic 40.0%
AGCTATCTTGGAGATCTTGT+GGG + chr4.2:79829478-79829497 None:intergenic 40.0%
AGTGAAGCCTATAGGATCTT+TGG + chr4.2:79829628-79829647 None:intergenic 40.0%
ATGCAAGAATTTATTGGGCC+GGG - chr4.2:79829738-79829757 MS.gene066643:intron 40.0%
ATGCTGTTAAACTCTGCTTC+AGG + chr4.2:79830516-79830535 None:intergenic 40.0%
ATGTTCTTGCCAATGATGTG+CGG - chr4.2:79828496-79828515 MS.gene066643:intron 40.0%
CACTGAAAAGTGAAGCCTAT+AGG + chr4.2:79829636-79829655 None:intergenic 40.0%
CCATCTCATGGAGGAAATAT+GGG - chr4.2:79829932-79829951 MS.gene066643:CDS 40.0%
CCATTCTTAGATGAAGAGCA+AGG + chr4.2:79829136-79829155 None:intergenic 40.0%
CCCTGGAAGGAATAACATTT+AGG + chr4.2:79830411-79830430 None:intergenic 40.0%
CCTTGCTCTTCATCTAAGAA+TGG - chr4.2:79829133-79829152 MS.gene066643:intron 40.0%
CTTAGATGAAGAGCAAGGTA+GGG + chr4.2:79829131-79829150 None:intergenic 40.0%
CTTGGATTCAAGGCTATCAA+AGG - chr4.2:79828905-79828924 MS.gene066643:intron 40.0%
GATGCAAGAATTTATTGGGC+CGG - chr4.2:79829737-79829756 MS.gene066643:intron 40.0%
GTATTTCTTGTGTCCTGCAT+AGG + chr4.2:79830442-79830461 None:intergenic 40.0%
TACCATGAAAGAAAGATGGC+TGG - chr4.2:79829275-79829294 MS.gene066643:CDS 40.0%
TCAGAATTCATTAGGACAGC+CGG - chr4.2:79830551-79830570 MS.gene066643:CDS 40.0%
TCCCTACTGAAATCCAAACA+AGG + chr4.2:79827865-79827884 None:intergenic 40.0%
TCTTAGATGAAGAGCAAGGT+AGG + chr4.2:79829132-79829151 None:intergenic 40.0%
TGCTGTTAAACTCTGCTTCA+GGG + chr4.2:79830515-79830534 None:intergenic 40.0%
! AAAGCTTCTTTTCCACCCAA+TGG + chr4.2:79829770-79829789 None:intergenic 40.0%
! ATGTCATTTGGGGAGATGAA+AGG - chr4.2:79828043-79828062 MS.gene066643:CDS 40.0%
! CCCTTAATCTCAAATGGGTT+TGG - chr4.2:79827520-79827539 MS.gene066643:intron 40.0%
! GATGTTTTCATGCCATCTCA+TGG - chr4.2:79829920-79829939 MS.gene066643:CDS 40.0%
! TTTTACGCAGCTTGGATTCA+AGG - chr4.2:79828895-79828914 MS.gene066643:intron 40.0%
!! AGCCTTGTTTGGATTTCAGT+AGG - chr4.2:79827860-79827879 MS.gene066643:intron 40.0%
!! CTATAGGCTTCACTTTTCAG+TGG - chr4.2:79829634-79829653 MS.gene066643:intron 40.0%
!! GAAAATGGTTCTAGTTCTCC+AGG + chr4.2:79829848-79829867 None:intergenic 40.0%
!! GCCTTGTTTGGATTTCAGTA+GGG - chr4.2:79827861-79827880 MS.gene066643:intron 40.0%
!! GTTAGGGTATAAGCCTTGTT+TGG - chr4.2:79827849-79827868 MS.gene066643:intron 40.0%
!! TGTTTTGCTTTTACGCAGCT+TGG - chr4.2:79828887-79828906 MS.gene066643:intron 40.0%
!! TTATGGTGGTTGTTTCTGGA+GGG - chr4.2:79827932-79827951 MS.gene066643:CDS 40.0%
!!! AGTTTTGGAAGCAACCAGAT+GGG - chr4.2:79827827-79827846 MS.gene066643:intron 40.0%
AAGGAAGATCTTGCATTGCC+TGG - chr4.2:79829827-79829846 MS.gene066643:CDS 45.0%
ATAGTCATACCTGAAGCGCA+TGG + chr4.2:79829958-79829977 None:intergenic 45.0%
CAACTTGGAGTGACATGAAG+AGG + chr4.2:79828579-79828598 None:intergenic 45.0%
CCCATATTTCCTCCATGAGA+TGG + chr4.2:79829935-79829954 None:intergenic 45.0%
CCTTCTCTGTTGAACTGCAA+AGG + chr4.2:79828867-79828886 None:intergenic 45.0%
CCTTTGCAGTTCAACAGAGA+AGG - chr4.2:79828864-79828883 MS.gene066643:intron 45.0%
CTCTTCATGTCACTCCAAGT+TGG - chr4.2:79828577-79828596 MS.gene066643:CDS 45.0%
GACCAGCCATCTTTCTTTCA+TGG + chr4.2:79829280-79829299 None:intergenic 45.0%
GACTTGGAGGAGGAAGAAAA+GGG - chr4.2:79827784-79827803 MS.gene066643:intron 45.0%
GAGATCTTGTGGGAATAAGC+TGG + chr4.2:79829468-79829487 None:intergenic 45.0%
GATCTTCCTTTCAAGAGCTC+CGG + chr4.2:79829245-79829264 None:intergenic 45.0%
GCCATCTCATGGAGGAAATA+TGG - chr4.2:79829931-79829950 MS.gene066643:CDS 45.0%
GCGAAACCAGTTCAAGAACT+TGG - chr4.2:79829071-79829090 MS.gene066643:intron 45.0%
GCTGAGAGAATGCAAAGCAA+AGG - chr4.2:79829104-79829123 MS.gene066643:intron 45.0%
GTAACATCCATGGCAGTCAA+GGG + chr4.2:79829308-79829327 None:intergenic 45.0%
GTTAAACTCTGCTTCAGGGA+GGG + chr4.2:79830511-79830530 None:intergenic 45.0%
TCAAACTCAGGACTTAGACC+AGG + chr4.2:79829191-79829210 None:intergenic 45.0%
TCTTCAGAAGCTTCGATGCA+AGG - chr4.2:79828941-79828960 MS.gene066643:CDS 45.0%
TGCAAGAATTTATTGGGCCG+GGG - chr4.2:79829739-79829758 MS.gene066643:intron 45.0%
TGTTAAACTCTGCTTCAGGG+AGG + chr4.2:79830512-79830531 None:intergenic 45.0%
TGTTCTTGCCAATGATGTGC+GGG - chr4.2:79828497-79828516 MS.gene066643:intron 45.0%
TTATTCCTTCCAGGGTCACA+GGG - chr4.2:79830416-79830435 MS.gene066643:CDS 45.0%
TTGCAAGCAAAGGTTCCTAG+TGG - chr4.2:79827757-79827776 MS.gene066643:intron 45.0%
! ACACTTGATGACAAAGCCAG+TGG - chr4.2:79828542-79828561 MS.gene066643:intron 45.0%
! CGAAGCTTCTGAAGATCTGA+AGG + chr4.2:79828936-79828955 None:intergenic 45.0%
! CTCTGCTCACCTATGTTTTC+AGG - chr4.2:79829025-79829044 MS.gene066643:intron 45.0%
! GTTTTCATGCCATCTCATGG+AGG - chr4.2:79829923-79829942 MS.gene066643:CDS 45.0%
! TGACTTGGAGGAGGAAGAAA+AGG - chr4.2:79827783-79827802 MS.gene066643:intron 45.0%
! TGTCACGAAGTATCCTGTTC+CGG - chr4.2:79830602-79830621 MS.gene066643:CDS 45.0%
! TTGATGACAAAGCCAGTGGA+GGG - chr4.2:79828546-79828565 MS.gene066643:intron 45.0%
!! CTTATGGTGGTTGTTTCTGG+AGG - chr4.2:79827931-79827950 MS.gene066643:CDS 45.0%
!! TAGGATTCTTGGTGCTGCTT+TGG - chr4.2:79827999-79828018 MS.gene066643:CDS 45.0%
!!! CTCTTTTGAGATAGCGCGAA+CGG + chr4.2:79828605-79828624 None:intergenic 45.0%
!!! GAGTTTTGGAAGCAACCAGA+TGG - chr4.2:79827826-79827845 MS.gene066643:intron 45.0%
AAATATGGGCCATGCGCTTC+AGG - chr4.2:79829946-79829965 MS.gene066643:CDS 50.0%
ACCTTGCTAGTCCTGAGTTC+CGG + chr4.2:79830573-79830592 None:intergenic 50.0%
CTTGGAGTGACATGAAGAGG+AGG + chr4.2:79828576-79828595 None:intergenic 50.0%
GAATGAGAGGAGAGAACGAC+CGG - chr4.2:79829223-79829242 MS.gene066643:intron 50.0%
GAGGAGGAAGAAAAGGGTGA+GGG - chr4.2:79827790-79827809 MS.gene066643:intron 50.0%
GCAAGAATTTATTGGGCCGG+GGG - chr4.2:79829740-79829759 MS.gene066643:intron 50.0%
GGCTTATACCCTAACCCATC+TGG + chr4.2:79827844-79827863 None:intergenic 50.0%
GGTAACATCCATGGCAGTCA+AGG + chr4.2:79829309-79829328 None:intergenic 50.0%
GGTTCCTAGTGGAGTTGACT+TGG - chr4.2:79827768-79827787 MS.gene066643:intron 50.0%
GTTATTCCTTCCAGGGTCAC+AGG - chr4.2:79830415-79830434 MS.gene066643:CDS 50.0%
TCCTCCAAGTCAACTCCACT+AGG + chr4.2:79827775-79827794 None:intergenic 50.0%
TCTTTGTCCCTTGACTGCCA+TGG - chr4.2:79829298-79829317 MS.gene066643:CDS 50.0%
TGACTGCCATGGATGTTACC+AGG - chr4.2:79829309-79829328 MS.gene066643:CDS 50.0%
TGGAAAAGGATGTGTGGGTG+CGG - chr4.2:79829153-79829172 MS.gene066643:intron 50.0%
! AACTCAGGACTAGCAAGGTC+GGG - chr4.2:79830574-79830593 MS.gene066643:CDS 50.0%
! CTTGATGACAAAGCCAGTGG+AGG - chr4.2:79828545-79828564 MS.gene066643:intron 50.0%
! GAACTCAGGACTAGCAAGGT+CGG - chr4.2:79830573-79830592 MS.gene066643:CDS 50.0%
! TGGAAGCAACCAGATGGGTT+AGG - chr4.2:79827832-79827851 MS.gene066643:intron 50.0%
!! GGAAGCAACCAGATGGGTTA+GGG - chr4.2:79827833-79827852 MS.gene066643:intron 50.0%
!! TAGTGGAGTTGACTTGGAGG+AGG - chr4.2:79827774-79827793 MS.gene066643:intron 50.0%
!! TCCTAGTGGAGTTGACTTGG+AGG - chr4.2:79827771-79827790 MS.gene066643:intron 50.0%
CATTAGGACAGCCGGAACTC+AGG - chr4.2:79830559-79830578 MS.gene066643:CDS 55.0%
CGAAACCACCCGCACATCAT+TGG + chr4.2:79828508-79828527 None:intergenic 55.0%
CTGGAGGGATGAATCAGCAG+AGG - chr4.2:79827947-79827966 MS.gene066643:CDS 55.0%
GAACGACCGGAGCTCTTGAA+AGG - chr4.2:79829236-79829255 MS.gene066643:intron 55.0%
GAGTCGTTGCACATGCACTC+CGG + chr4.2:79830624-79830643 None:intergenic 55.0%
GGAGGAGGAAGAAAAGGGTG+AGG - chr4.2:79827789-79827808 MS.gene066643:intron 55.0%
TAGCGCGAACGGATCCAACT+TGG + chr4.2:79828594-79828613 None:intergenic 55.0%
TCTTGCCAATGATGTGCGGG+TGG - chr4.2:79828500-79828519 MS.gene066643:intron 55.0%
TTGCACATGCACTCCGGAAC+AGG + chr4.2:79830618-79830637 None:intergenic 55.0%
AAGGATGTGTGGGTGCGGAC+TGG - chr4.2:79829158-79829177 MS.gene066643:intron 60.0%
AGAGGAGGACTTCCCTCCAC+TGG + chr4.2:79828561-79828580 None:intergenic 60.0%
ATAGGCCCTGTGACCCTGGA+AGG + chr4.2:79830424-79830443 None:intergenic 60.0%
TCCACCCAATGGTTGTCCCC+CGG + chr4.2:79829759-79829778 None:intergenic 60.0%
! CAGGACTAGCAAGGTCGGGA+GGG - chr4.2:79830578-79830597 MS.gene066643:CDS 60.0%
! GCCGGAACTCAGGACTAGCA+AGG - chr4.2:79830569-79830588 MS.gene066643:CDS 60.0%
! TCAGGACTAGCAAGGTCGGG+AGG - chr4.2:79830577-79830596 MS.gene066643:CDS 60.0%
!! GTGCGGACTGGTTGTCTACC+TGG - chr4.2:79829170-79829189 MS.gene066643:intron 60.0%
CAGGGTCACAGGGCCTATGC+AGG - chr4.2:79830426-79830445 MS.gene066643:CDS 65.0%
CTGCATAGGCCCTGTGACCC+TGG + chr4.2:79830428-79830447 None:intergenic 65.0%
TGGGCCGGGGGACAACCATT+GGG - chr4.2:79829752-79829771 MS.gene066643:intron 65.0%
TTGGGCCGGGGGACAACCAT+TGG - chr4.2:79829751-79829770 MS.gene066643:intron 65.0%
GCCGGGGGACAACCATTGGG+TGG - chr4.2:79829755-79829774 MS.gene066643:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 79827223 79830659 79827223 ID=MS.gene066643
chr4.2 mRNA 79827223 79830659 79827223 ID=MS.gene066643.t1;Parent=MS.gene066643
chr4.2 exon 79829818 79830659 79829818 ID=MS.gene066643.t1.exon1;Parent=MS.gene066643.t1
chr4.2 CDS 79829818 79830659 79829818 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
chr4.2 exon 79829259 79829424 79829259 ID=MS.gene066643.t1.exon2;Parent=MS.gene066643.t1
chr4.2 CDS 79829259 79829424 79829259 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
chr4.2 exon 79828920 79829009 79828920 ID=MS.gene066643.t1.exon3;Parent=MS.gene066643.t1
chr4.2 CDS 79828920 79829009 79828920 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
chr4.2 exon 79828552 79828835 79828552 ID=MS.gene066643.t1.exon4;Parent=MS.gene066643.t1
chr4.2 CDS 79828552 79828835 79828552 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
chr4.2 exon 79827915 79828173 79827915 ID=MS.gene066643.t1.exon5;Parent=MS.gene066643.t1
chr4.2 CDS 79827915 79828173 79827915 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
chr4.2 exon 79827223 79827453 79827223 ID=MS.gene066643.t1.exon6;Parent=MS.gene066643.t1
chr4.2 CDS 79827223 79827453 79827223 ID=cds.MS.gene066643.t1;Parent=MS.gene066643.t1
Gene Sequence

>MS.gene066643

ATGGCTAAGTCAAAGAACAATGCAAAGAAACTCTCATACATCTCAGTTCCTTCACAAATAATCAATTCCATTTCATCTTCTTCTATTCAATCATTACTTGATGATCAAACTCCAAAGAAATCTTCAACAAAAATTGCTTCAAACTTCTTCTTCACCACCTTAACAAAATGGAAAAACCCTTCAAGATTGTTCTTCTTTTTCACACTCTTTTTTCTTGGTGGTTTTGCTTTGTTTATGATTGGTTTCAATCTTAAAACACCGTTTTTTAAAACCCCATGTGCTACAAATGTTGAAAAGCCCTTAATCTCAAATGGGTTTGGTTCAAAATCTGAACTTAAGGTTGTTTCAATGAATAAAAATGAGATTTTTAATGGTGATGTTTTGGTAAAATCTGAACTTGGGGTTGTTCAAAACCCTTCAATCTCAAATGGGTTTGATTCAAAATCTGAACTTGGTGCTGTTTCAATGAATAAAGATGAGGTTTTGAATGATGTTAGGTTGTCAAATTCACTTCCTTTGATTGGTAATGTAATGTTGCAAGCAAAGGTTCCTAGTGGAGTTGACTTGGAGGAGGAAGAAAAGGGTGAGGGAGTTGAAAAGAGTGAGTTTTGGAAGCAACCAGATGGGTTAGGGTATAAGCCTTGTTTGGATTTCAGTAGGGATTATAGAAGAGAAAGTGATGGGATTGTGAAGAATAGAAAAAGGTATCTTATGGTGGTTGTTTCTGGAGGGATGAATCAGCAGAGGAATCAAATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTGCTTTGGTTGTTCCTATTTTGCAAGTTAATGTCATTTGGGGAGATGAAAGGTAAAGTTTACTATTCTTCTTGTTCATGGTTTGTGATATTGTAAGTGATGATTTTAATCAATGAAGATTTATGTAAGGTTTAGTTATTACAATTTTGGACTTAATCTAAGCCTAAGCATAGATTGATCTTGTTTCAATATTGTTTGAAAGACCAAATTTGGATATATATTTAGCTGTTACAAATTTGGACTGACTCTTGAGAAAGAACAACTTTGGAGCTTAAGCTTTAAGCATAGTTCTATTCTAGCTATAGTTTCAATCTTTTTGTTCTTGATACTGGTTATGTTTCGTTGTTAATGTTGATTAATTTTTTTTCCTTATTTGTGAATGCTAGAAGAGAGTTTTCTGTAATTTATGATGTTTTTCCTTATTGGTTTTGGTTTTGTTTTGCAGTGAATTTGCTGATATATTTGATTTTGAGCACTTCAAGAATGTTCTTGCCAATGATGTGCGGGTGGTTTCGTCATTGCCATCAACACACTTGATGACAAAGCCAGTGGAGGGAAGTCCTCCTCTTCATGTCACTCCAAGTTGGATCCGTTCGCGCTATCTCAAAAGAGTAAGATTTTTTTGTTAGTTACTCTTCTTATGTTTTAGCATCTTTAGGTTTCAGTCCTAAGTTTTTGTCTACTTTTGCGTTGAAAAATTTCACATGATGATTGTCTCTCTGATGAGAAAGTGTGTTAACTTGTATATATAATGTTAACTAATAAAAGCTTTGACTTCTGTGGAAATAAAAATTTGGGCACTGAGTGATTGTTGTTCTTGTTGAACTAGAGTTTAACATGGTCTTTGTTGTCCTTTGCAGTTCAACAGAGAAGGTGTTTTGCTTTTACGCAGCTTGGATTCAAGGCTATCAAAGGATCTTCCTTCAGATCTTCAGAAGCTTCGATGCAAGGTATTGTGAAGTGTCTATAGTATCTTCATCTGTGTAACAATCGGTAGCATATTCTAAATGTTCTCTGCTCACCTATGTTTTCAGGTTGCTTTTAGTGCATTGAGATTTGCGAAACCAGTTCAAGAACTTGGAAACAAGATTGCTGAGAGAATGCAAAGCAAAGGACCCTACCTTGCTCTTCATCTAAGAATGGAAAAGGATGTGTGGGTGCGGACTGGTTGTCTACCTGGTCTAAGTCCTGAGTTTGACGAGATTGTAAAGAATGAGAGGAGAGAACGACCGGAGCTCTTGAAAGGAAGATCAAACATGACTTACCATGAAAGAAAGATGGCTGGTCTTTGTCCCTTGACTGCCATGGATGTTACCAGGTAAATATTAAATTCTTCTACTTTTGTATAGGAGTCTGTTTGGATTCTCTTATTCGAGTTTATTACTGGCATTTACACTTATGAAACTATTTGGGAGAGCTTATGGAAACTTATGAAACGTCCATAAACTGTTTCCAGCTTATTCCCACAAGATCTCCAAGATAGCTTATGAAATCAGTTTAGTTTATATAAAAATAGTTCGATTTTATTTTATCTTTTATTATAGAAATAGCGTATACATAAACACTTATATGATAAACACTTATGCTACAAGCGCTTAATTAAGTTGTTTATCCAAAGATCCTATAGGCTTCACTTTTCAGTGGTTTTCTGAATATTAGTGTGACAGAGTTTTGTTTATTTGTTTTTGATTTATAGGTTGCTTAAAGCTTTAGGAGCTCAAAAAGATGCAAGAATTTATTGGGCCGGGGGACAACCATTGGGTGGAAAAGAAGCTTTGCATCCATTAATCCATGAGTTTCCACATCTTTATAATAAGGAAGATCTTGCATTGCCTGGAGAACTAGAACCATTTTCGAAGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTTTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCATGCGCTTCAGGTATGACTATATAATGACAGCAAGAGTGCTTTGCAAATAAGATACTCACATAAGCATTGATGATTGTTGCATAACTATATTGTAATCATGCTTACATATATTGGCCATGCATTCATATATACTATTCACACATATTTTATAAGAAAAGATTGCGACCTTTCACCTTTAGTCCCTATAAAATTTGTACTCACCTTTAGTCTTCATATTTAATAAAAATAGTGTGCTTTTAGTAATTAAAAAGGACTGATGATACAATCAATTCTTTTAGGGATGTTGATGAGTCGTGATTTTATAGGACCAAAAACATATTTTCTCCATAAATGGTAATCATGCAATTAAGATAGTCACATTGATGGATGTTGCATAAATATATTGTAAATGTAATCATGCATACACTTTTTTAATGTAGATTCTTCATTCGACAATTTCTAATATCTGAACCTAAATGTTATTCCTTCCAGGGTCACAGGGCCTATGCAGGACACAAGAAATACATAACCCCAAACAAAAGACAAATGCATCCCTATTTTCTGAATTCTTCCCTCCCTGAAGCAGAGTTTAACAGCATTGTCAAAGAATTGCATCAGAATTCATTAGGACAGCCGGAACTCAGGACTAGCAAGGTCGGGAGGGATGTCACGAAGTATCCTGTTCCGGAGTGCATGTGCAACGACTCACATAACGATTCGTAG
Protein sequence

>MS.gene066643.t1

MAKSKNNAKKLSYISVPSQIINSISSSSIQSLLDDQTPKKSSTKIASNFFFTTLTKWKNPSRLFFFFTLFFLGGFALFMIGFNLKTPFFKTPCATNVEKPLISNGFGSKSELKVVSMNKNEIFNGDVLVKSELGVVQNPSISNGFDSKSELGAVSMNKDEVLNDVRLSNSLPLIGNVMLQAKVPSGVDLEEEEKGEGVEKSEFWKQPDGLGYKPCLDFSRDYRRESDGIVKNRKRYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFADIFDFEHFKNVLANDVRVVSSLPSTHLMTKPVEGSPPLHVTPSWIRSRYLKRFNREGVLLLRSLDSRLSKDLPSDLQKLRCKVAFSALRFAKPVQELGNKIAERMQSKGPYLALHLRMEKDVWVRTGCLPGLSPEFDEIVKNERRERPELLKGRSNMTYHERKMAGLCPLTAMDVTRLLKALGAQKDARIYWAGGQPLGGKEALHPLIHEFPHLYNKEDLALPGELEPFSKKASLMAAIDYIVSEKSDVFMPSHGGNMGHALQGHRAYAGHKKYITPNKRQMHPYFLNSSLPEAEFNSIVKELHQNSLGQPELRTSKVGRDVTKYPVPECMCNDSHNDS