AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066723

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066723.t1 MTR_4g091200 91.915 470 38 0 1 470 59 528 0.0 888
MS.gene066723.t1 MTR_6g017205 90.598 468 44 0 1 468 59 526 0.0 863
MS.gene066723.t1 MTR_4g057990 82.303 469 79 2 1 469 59 523 0.0 781
MS.gene066723.t1 MTR_4g091190 80.128 468 91 1 1 468 63 528 0.0 765
MS.gene066723.t1 MTR_4g088355 78.252 469 102 0 1 469 66 534 0.0 756
MS.gene066723.t1 MTR_2g023590 79.281 473 96 1 1 473 1 471 0.0 756
MS.gene066723.t1 MTR_4g091170 72.767 459 107 1 10 468 102 542 0.0 706
MS.gene066723.t1 MTR_4g091150 63.034 468 171 2 1 466 71 538 0.0 596
MS.gene066723.t1 MTR_2g031560 53.863 466 212 2 3 467 59 522 0.0 539
MS.gene066723.t1 MTR_2g031590 53.079 471 212 4 3 467 64 531 2.09e-178 511
MS.gene066723.t1 MTR_4g094498 53.333 465 213 3 3 467 73 533 4.21e-173 498
MS.gene066723.t1 MTR_2g031390 49.679 467 227 6 4 466 65 527 2.43e-165 478
MS.gene066723.t1 MTR_4g094488 53.488 473 211 4 3 468 67 537 5.54e-165 477
MS.gene066723.t1 MTR_4g094495 52.542 472 215 4 4 468 71 540 2.87e-164 475
MS.gene066723.t1 MTR_4g094492 50.959 469 224 3 4 467 68 535 4.64e-163 472
MS.gene066723.t1 MTR_4g094520 51.173 469 218 6 4 467 69 531 9.54e-162 468
MS.gene066723.t1 MTR_4g094480 51.812 469 221 3 4 468 68 535 2.11e-161 468
MS.gene066723.t1 MTR_4g094478 52.350 468 218 3 4 467 68 534 2.63e-161 468
MS.gene066723.t1 MTR_3g009820 48.276 464 233 5 4 466 63 520 9.07e-160 463
MS.gene066723.t1 MTR_4g094518 49.893 469 221 7 5 466 67 528 9.12e-159 461
MS.gene066723.t1 MTR_2g031490 50.851 470 221 6 3 466 71 536 8.76e-158 458
MS.gene066723.t1 MTR_2g031510 49.895 475 218 6 3 466 73 538 5.11e-156 454
MS.gene066723.t1 MTR_3g009760 47.198 464 238 5 4 466 63 520 1.43e-153 447
MS.gene066723.t1 MTR_3g009880 48.276 464 233 5 4 466 61 518 8.02e-153 446
MS.gene066723.t1 MTR_4g094418 50.113 441 212 4 40 473 1 440 1.90e-152 447
MS.gene066723.t1 MTR_2g031420 50.427 468 225 4 3 469 67 528 2.60e-152 444
MS.gene066723.t1 MTR_2g031460 49.138 464 229 4 3 465 67 524 2.38e-150 439
MS.gene066723.t1 MTR_4g094515 51.380 471 220 6 3 468 74 540 9.65e-150 456
MS.gene066723.t1 MTR_4g094515 51.282 468 221 5 3 467 686 1149 2.50e-148 452
MS.gene066723.t1 MTR_1g076830 37.708 480 283 8 3 473 68 540 5.77e-100 310
MS.gene066723.t1 MTR_2g031600 63.889 180 65 0 3 182 65 244 1.15e-70 230
MS.gene066723.t1 MTR_2g031600 51.875 160 76 1 308 467 241 399 3.04e-53 184
MS.gene066723.t1 MTR_3g009850 48.661 224 112 3 244 466 5 226 9.93e-65 209
MS.gene066723.t1 MTR_2g084370 45.946 111 38 2 64 174 1 89 1.67e-28 108
MS.gene066723.t1 MTR_2g084405 44.304 79 44 0 64 142 1 79 4.15e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066723.t1 AT4G20800 55.365 466 205 2 4 469 64 526 1.74e-178 511
MS.gene066723.t1 AT1G26420 54.741 464 204 4 4 466 65 523 1.64e-175 503
MS.gene066723.t1 AT1G26390 55.699 465 200 4 4 466 65 525 9.26e-175 501
MS.gene066723.t1 AT1G26380 54.957 464 203 4 4 466 65 523 2.71e-174 500
MS.gene066723.t1 AT1G30720 53.419 468 213 3 2 468 63 526 1.53e-172 495
MS.gene066723.t1 AT1G34575 54.390 467 201 4 3 466 65 522 2.90e-171 492
MS.gene066723.t1 AT1G30730 52.137 468 219 3 2 468 62 525 6.88e-170 489
MS.gene066723.t1 AT1G26400 52.991 468 214 6 4 470 65 527 2.44e-169 487
MS.gene066723.t1 AT1G30710 53.814 472 203 7 3 469 68 529 3.09e-168 484
MS.gene066723.t1 AT1G26410 54.741 464 204 5 4 466 89 547 4.73e-168 485
MS.gene066723.t1 AT1G30700 51.709 468 217 5 3 466 62 524 1.81e-163 473
MS.gene066723.t1 AT5G44400 49.679 467 227 6 4 466 72 534 1.48e-158 460
MS.gene066723.t1 AT5G44390 50.320 469 225 5 4 467 74 539 2.26e-158 460
MS.gene066723.t1 AT2G34810 49.574 470 231 3 3 467 70 538 6.50e-157 456
MS.gene066723.t1 AT2G34790 49.359 468 227 5 4 468 70 530 1.55e-156 455
MS.gene066723.t1 AT4G20840 50.638 470 219 7 3 467 73 534 1.85e-155 452
MS.gene066723.t1 AT1G30760 49.359 468 225 6 4 467 72 531 1.41e-153 447
MS.gene066723.t1 AT1G30760 49.359 468 225 6 4 467 90 549 1.52e-153 448
MS.gene066723.t1 AT1G11770 48.614 469 230 6 3 467 67 528 2.03e-149 437
MS.gene066723.t1 AT1G01980 50.737 475 216 9 3 467 67 533 6.00e-148 433
MS.gene066723.t1 AT5G44380 48.927 466 232 4 4 466 75 537 1.43e-147 432
MS.gene066723.t1 AT5G44380 48.927 466 232 4 4 466 115 577 2.51e-147 433
MS.gene066723.t1 AT4G20820 47.639 466 230 5 3 466 71 524 1.36e-145 427
MS.gene066723.t1 AT4G20860 47.588 456 233 4 12 466 77 527 1.50e-145 427
MS.gene066723.t1 AT5G44360 48.180 467 230 7 4 466 70 528 2.86e-142 419
MS.gene066723.t1 AT5G44360 48.180 467 230 7 4 466 86 544 4.08e-142 419
MS.gene066723.t1 AT4G20830 48.936 470 227 7 3 467 74 535 3.87e-140 413
MS.gene066723.t1 AT4G20830 48.936 470 227 7 3 467 74 535 1.31e-139 413
MS.gene066723.t1 AT5G44440 46.170 470 240 5 3 466 65 527 7.77e-139 410
MS.gene066723.t1 AT5G44440 46.170 470 240 5 3 466 135 597 1.66e-137 409
MS.gene066723.t1 AT5G44410 48.408 471 224 9 3 466 69 527 1.97e-135 401
MS.gene066723.t1 AT1G30740 47.234 470 237 7 3 467 67 530 9.53e-131 389
MS.gene066723.t1 AT1G30740 47.234 470 237 7 3 467 64 527 1.04e-130 389

Find 85 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCTAATGTTGTTCAAAT+TGG 0.228780 4.2:-21061602 MS.gene066723:CDS
AAGCTGAATGTTGTTGCTTT+TGG 0.230709 4.2:+21062174 None:intergenic
TTTGACATTGCTAAAACTTA+TGG 0.257856 4.2:-21060996 MS.gene066723:CDS
GGAAATTTGATGAGAAAATT+TGG 0.258916 4.2:-21061902 MS.gene066723:CDS
TGGCACATTTAACTGTTTCT+TGG 0.267359 4.2:+21062194 None:intergenic
AATATATCTTTATGCAGTTT+TGG 0.269973 4.2:+21061635 None:intergenic
AGTAAGGAATTCCCCGAATT+AGG 0.285169 4.2:-21061500 MS.gene066723:CDS
AACAAGCTTGCTTTCCCATC+TGG 0.299204 4.2:-21061974 MS.gene066723:CDS
ACTTTGGTTCAAACCTAATT+CGG 0.311377 4.2:+21061487 None:intergenic
TGACCAATTTGAACAACATT+AGG 0.312133 4.2:+21061599 None:intergenic
TCTTTCATCTTTCCACCATA+TGG 0.316468 4.2:+21061248 None:intergenic
ATAGATTCTAGAGCTTCTTT+AGG 0.318699 4.2:+21061317 None:intergenic
TCTAGAGCTTCTTTAGGAAT+AGG 0.321024 4.2:+21061323 None:intergenic
GTTATAATTGCCAAAGGTTT+CGG 0.327951 4.2:+21062238 None:intergenic
GGTGGTGGTGGTGCTAGTTT+TGG 0.331271 4.2:-21061779 MS.gene066723:CDS
AAGAAGCTCTAGAATCTATA+TGG 0.334429 4.2:-21061313 MS.gene066723:CDS
CTTTGGTTCAAACCTAATTC+GGG 0.343696 4.2:+21061488 None:intergenic
GGGAGGCATTCCTCAATTAT+AGG 0.357483 4.2:-21061061 MS.gene066723:CDS
CATTTCTCTGGTGGTGGGTA+TGG 0.360817 4.2:-21061923 MS.gene066723:CDS
AATTATAGGGATGCTGATAT+TGG 0.376157 4.2:-21061047 MS.gene066723:CDS
GTAGTGCAAGTTAGTTTCAT+TGG 0.379275 4.2:-21061563 MS.gene066723:CDS
GTGATTGTCTTTCAATGCCT+TGG 0.383003 4.2:-21061466 MS.gene066723:CDS
TCAAATCTCGTTACATTACT+TGG 0.393941 4.2:+21061014 None:intergenic
ACGATAACCTGTGGAGTTAT+CGG 0.397449 4.2:+21061725 None:intergenic
TAAATTGGTTGAGGATCCTT+TGG 0.399190 4.2:+21061377 None:intergenic
TACTCCTCTTGAAGCATTGT+TGG 0.402290 4.2:-21061405 MS.gene066723:CDS
TTCATATCTAAAGAAATTCT+CGG 0.418836 4.2:+21060914 None:intergenic
GAAATTCTCGGGATCAACTT+TGG 0.424393 4.2:+21060926 None:intergenic
GGTGGTCATTTCTCTGGTGG+TGG 0.426644 4.2:-21061929 MS.gene066723:CDS
TCAACAGCATGGGTTGAATC+AGG 0.427451 4.2:-21062040 MS.gene066723:CDS
ACAAGCTTGCTTTCCCATCT+GGG 0.436569 4.2:-21061973 MS.gene066723:CDS
TCTTTCATGGAAACTTCAAT+TGG 0.441584 4.2:-21061750 MS.gene066723:CDS
GGAGGCATTCCTCAATTATA+GGG 0.443623 4.2:-21061060 MS.gene066723:CDS
CTTATGCAATGGAATCCATA+TGG 0.450449 4.2:-21061263 MS.gene066723:CDS
TATTCTTGATAGAAAATCAA+TGG 0.458066 4.2:-21061828 MS.gene066723:CDS
GGTGCTGGTGGTCATTTCTC+TGG 0.459504 4.2:-21061935 MS.gene066723:CDS
TCTGGGGTCTGCTTTACTCT+AGG 0.464774 4.2:-21061956 MS.gene066723:CDS
AGCAACAACACCGAAACCTT+TGG 0.466175 4.2:-21062248 MS.gene066723:CDS
CTCGGAGAATTCGAAACATA+AGG 0.468696 4.2:+21061083 None:intergenic
GTCTGCTTTACTCTAGGTGC+TGG 0.472600 4.2:-21061950 MS.gene066723:CDS
CATATGGATTCCATTGCATA+AGG 0.477148 4.2:+21061264 None:intergenic
AGTGGCGGTCACGACTTTGA+AGG 0.481864 4.2:-21062142 MS.gene066723:CDS
CAACTGATGTCGTTTACAAA+TGG 0.490862 4.2:-21061670 MS.gene066723:CDS
GTGGTCATTTCTCTGGTGGT+GGG 0.501321 4.2:-21061928 MS.gene066723:CDS
AGAGTAAAGCAGACCCCAGA+TGG 0.503056 4.2:+21061959 None:intergenic
AACAAGTCAAGTATAACAAA+AGG 0.504620 4.2:+21062097 None:intergenic
AACAAGTTTCCTACTCTATG+AGG 0.510558 4.2:+21061200 None:intergenic
TGCTTTACTCTAGGTGCTGG+TGG 0.512366 4.2:-21061947 MS.gene066723:CDS
TCATATCTAAAGAAATTCTC+GGG 0.512401 4.2:+21060915 None:intergenic
TGGGCTATAAGAGGTGGTGG+TGG 0.518497 4.2:-21061791 MS.gene066723:CDS
TTTCCTACTCTATGAGGAAA+CGG 0.519543 4.2:+21061206 None:intergenic
GCTTCAAGAGGAGTACCAAT+TGG 0.520632 4.2:+21061413 None:intergenic
ATTCTTGATAGAAAATCAAT+GGG 0.522100 4.2:-21061827 MS.gene066723:CDS
GTGAGAATTTCCTTATGCAA+TGG 0.526102 4.2:-21061274 MS.gene066723:CDS
GTTGTTCAAATTGGTCAAGA+GGG 0.530168 4.2:-21061593 MS.gene066723:CDS
ATTGGTTCCGATAACTCCAC+AGG 0.531147 4.2:-21061732 MS.gene066723:CDS
ATATGGAAATTGATGATTGA+AGG 0.537491 4.2:-21061296 MS.gene066723:CDS
GACTGATACAATGATCTAGT+AGG 0.541767 4.2:+21060882 None:intergenic
TTTACAAGAATCAACAGCAT+GGG 0.543499 4.2:-21062050 MS.gene066723:CDS
GCATTGAAAGACAATCACTT+TGG 0.543501 4.2:+21061471 None:intergenic
TATCAGCATCCCTATAATTG+AGG 0.547264 4.2:+21061051 None:intergenic
TGTTGTTCAAATTGGTCAAG+AGG 0.558808 4.2:-21061594 MS.gene066723:CDS
AGCATTGTTGGAAGAACCAA+AGG 0.559301 4.2:-21061393 MS.gene066723:CDS
GCTGGTGGTCATTTCTCTGG+TGG 0.564457 4.2:-21061932 MS.gene066723:CDS
CAAGCTTGCTTTCCCATCTG+GGG 0.567506 4.2:-21061972 MS.gene066723:CDS
TGATTCAATACTTTAATCCG+TGG 0.570353 4.2:-21061175 MS.gene066723:CDS
TTTGCAGTTATAATTGCCAA+AGG 0.570639 4.2:+21062232 None:intergenic
TAATTGAGGAATGCCTCCCT+CGG 0.575705 4.2:+21061065 None:intergenic
TGAGGAAACGGTGTTTCTGA+TGG 0.577426 4.2:+21061218 None:intergenic
TCTTCCAACAATGCTTCAAG+AGG 0.594711 4.2:+21061401 None:intergenic
ACACCGTTTCCTCATAGAGT+AGG 0.600052 4.2:-21061209 MS.gene066723:CDS
AAAAGAGTGGAATTAATCCA+AGG 0.600902 4.2:+21061449 None:intergenic
ATTTACAAGAATCAACAGCA+TGG 0.603350 4.2:-21062051 MS.gene066723:CDS
TTATGTTTCGAATTCTCCGA+GGG 0.609571 4.2:-21061081 MS.gene066723:CDS
GTTGAATCAGGTGCAACAGT+TGG 0.611200 4.2:-21062028 MS.gene066723:CDS
TGTTTCGAATTCTCCGAGGG+AGG 0.634404 4.2:-21061078 MS.gene066723:CDS
GAGTAAAGCAGACCCCAGAT+GGG 0.637967 4.2:+21061960 None:intergenic
CAGCTTAGAATTCGAAGTGG+CGG 0.641139 4.2:-21062157 MS.gene066723:CDS
TTTGGTTCAAACCTAATTCG+GGG 0.645523 4.2:+21061489 None:intergenic
ATGCAATGGAATCCATATGG+TGG 0.646403 4.2:-21061260 MS.gene066723:CDS
ACATTGAAAACGATAACCTG+TGG 0.652393 4.2:+21061716 None:intergenic
CTTATGTTTCGAATTCTCCG+AGG 0.672859 4.2:-21061082 MS.gene066723:CDS
TCAGAAGAATCATTAGACCA+CGG 0.676978 4.2:+21061158 None:intergenic
GTGAAAAGGAATGTCAGTGA+AGG 0.694179 4.2:-21061695 MS.gene066723:CDS
ATTCAGCTTAGAATTCGAAG+TGG 0.701549 4.2:-21062160 MS.gene066723:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTATTTTTTTGATAAAT+TGG + chr4.2:21061793-21061812 None:intergenic 10.0%
!! ATTCTTGATAGAAAATCAAT+GGG - chr4.2:21061325-21061344 MS.gene066723:CDS 20.0%
!! TATTCTTGATAGAAAATCAA+TGG - chr4.2:21061324-21061343 MS.gene066723:CDS 20.0%
!! TTCATATCTAAAGAAATTCT+CGG + chr4.2:21062241-21062260 None:intergenic 20.0%
!!! AATATATCTTTATGCAGTTT+TGG + chr4.2:21061520-21061539 None:intergenic 20.0%
!!! ACTTTTAATTTTGATGAGTA+AGG - chr4.2:21061636-21061655 MS.gene066723:CDS 20.0%
!!! TTTTTTTGATAAATTGGTTG+AGG + chr4.2:21061787-21061806 None:intergenic 20.0%
! AACAAGTCAAGTATAACAAA+AGG + chr4.2:21061058-21061077 None:intergenic 25.0%
! GCAAAAATTGTTGATGTTAA+GGG - chr4.2:21061298-21061317 MS.gene066723:CDS 25.0%
! GGAAATTTGATGAGAAAATT+TGG - chr4.2:21061250-21061269 MS.gene066723:CDS 25.0%
! TCATATCTAAAGAAATTCTC+GGG + chr4.2:21062240-21062259 None:intergenic 25.0%
! TGCAAAAATTGTTGATGTTA+AGG - chr4.2:21061297-21061316 MS.gene066723:CDS 25.0%
! TTTGACATTGCTAAAACTTA+TGG - chr4.2:21062156-21062175 MS.gene066723:CDS 25.0%
!! AGTTTCATTGGTCAATTTTT+AGG - chr4.2:21061601-21061620 MS.gene066723:CDS 25.0%
!! ATATGGAAATTGATGATTGA+AGG - chr4.2:21061856-21061875 MS.gene066723:CDS 25.0%
!! TTATCGTTTTCAATGTGAAA+AGG - chr4.2:21061443-21061462 MS.gene066723:CDS 25.0%
AAAAGAGTGGAATTAATCCA+AGG + chr4.2:21061706-21061725 None:intergenic 30.0%
AATTATAGGGATGCTGATAT+TGG - chr4.2:21062105-21062124 MS.gene066723:CDS 30.0%
ATTTACAAGAATCAACAGCA+TGG - chr4.2:21061101-21061120 MS.gene066723:CDS 30.0%
CAACCTAATGTTGTTCAAAT+TGG - chr4.2:21061550-21061569 MS.gene066723:CDS 30.0%
GTTATAATTGCCAAAGGTTT+CGG + chr4.2:21060917-21060936 None:intergenic 30.0%
TCAAATCTCGTTACATTACT+TGG + chr4.2:21062141-21062160 None:intergenic 30.0%
TCTTTCATGGAAACTTCAAT+TGG - chr4.2:21061402-21061421 MS.gene066723:CDS 30.0%
TGACCAATTTGAACAACATT+AGG + chr4.2:21061556-21061575 None:intergenic 30.0%
TGATTCAATACTTTAATCCG+TGG - chr4.2:21061977-21061996 MS.gene066723:CDS 30.0%
TTTACAAGAATCAACAGCAT+GGG - chr4.2:21061102-21061121 MS.gene066723:CDS 30.0%
TTTGCAGTTATAATTGCCAA+AGG + chr4.2:21060923-21060942 None:intergenic 30.0%
! AACGTCATGTGAATTTTTCA+AGG - chr4.2:21062025-21062044 MS.gene066723:CDS 30.0%
! ACTTTGGTTCAAACCTAATT+CGG + chr4.2:21061668-21061687 None:intergenic 30.0%
!! AAGAAGCTCTAGAATCTATA+TGG - chr4.2:21061839-21061858 MS.gene066723:CDS 30.0%
!! ATAGATTCTAGAGCTTCTTT+AGG + chr4.2:21061838-21061857 None:intergenic 30.0%
!! TATGGAAGCAAGTTTTTCAA+AGG - chr4.2:21062174-21062193 MS.gene066723:CDS 30.0%
!!! AATGGGAGAAGATCTTTTTT+GGG - chr4.2:21061342-21061361 MS.gene066723:CDS 30.0%
!!! AATTCCACTCTTTTTTGGTA+TGG - chr4.2:21061712-21061731 MS.gene066723:CDS 30.0%
!!! GGATTAATTCCACTCTTTTT+TGG - chr4.2:21061707-21061726 MS.gene066723:CDS 30.0%
!!! GTTTTGGTGTTATTCTTTCA+TGG - chr4.2:21061389-21061408 MS.gene066723:CDS 30.0%
AACAAGTTTCCTACTCTATG+AGG + chr4.2:21061955-21061974 None:intergenic 35.0%
AATTGGTCAAGAGGGAAAAA+AGG - chr4.2:21061567-21061586 MS.gene066723:CDS 35.0%
ACATTGAAAACGATAACCTG+TGG + chr4.2:21061439-21061458 None:intergenic 35.0%
ATTCAGCTTAGAATTCGAAG+TGG - chr4.2:21060992-21061011 MS.gene066723:CDS 35.0%
CATATGGATTCCATTGCATA+AGG + chr4.2:21061891-21061910 None:intergenic 35.0%
CTTATGCAATGGAATCCATA+TGG - chr4.2:21061889-21061908 MS.gene066723:CDS 35.0%
GACTGATACAATGATCTAGT+AGG + chr4.2:21062273-21062292 None:intergenic 35.0%
GCATTGAAAGACAATCACTT+TGG + chr4.2:21061684-21061703 None:intergenic 35.0%
GTGAGAATTTCCTTATGCAA+TGG - chr4.2:21061878-21061897 MS.gene066723:CDS 35.0%
GTTGTTCAAATTGGTCAAGA+GGG - chr4.2:21061559-21061578 MS.gene066723:CDS 35.0%
TAAATTGGTTGAGGATCCTT+TGG + chr4.2:21061778-21061797 None:intergenic 35.0%
TATCAGCATCCCTATAATTG+AGG + chr4.2:21062104-21062123 None:intergenic 35.0%
TCAGAAGAATCATTAGACCA+CGG + chr4.2:21061997-21062016 None:intergenic 35.0%
TCTTTCATCTTTCCACCATA+TGG + chr4.2:21061907-21061926 None:intergenic 35.0%
TGTTGTTCAAATTGGTCAAG+AGG - chr4.2:21061558-21061577 MS.gene066723:CDS 35.0%
TTATGTTTCGAATTCTCCGA+GGG - chr4.2:21062071-21062090 MS.gene066723:CDS 35.0%
TTTCCTACTCTATGAGGAAA+CGG + chr4.2:21061949-21061968 None:intergenic 35.0%
! CAACTGATGTCGTTTACAAA+TGG - chr4.2:21061482-21061501 MS.gene066723:CDS 35.0%
! CTTTGGTTCAAACCTAATTC+GGG + chr4.2:21061667-21061686 None:intergenic 35.0%
! GTAGTGCAAGTTAGTTTCAT+TGG - chr4.2:21061589-21061608 MS.gene066723:CDS 35.0%
! TCTAGAGCTTCTTTAGGAAT+AGG + chr4.2:21061832-21061851 None:intergenic 35.0%
! TGGCACATTTAACTGTTTCT+TGG + chr4.2:21060961-21060980 None:intergenic 35.0%
! TTTGGTTCAAACCTAATTCG+GGG + chr4.2:21061666-21061685 None:intergenic 35.0%
!!! AAGCTGAATGTTGTTGCTTT+TGG + chr4.2:21060981-21061000 None:intergenic 35.0%
!!! CAATGGGAGAAGATCTTTTT+TGG - chr4.2:21061341-21061360 MS.gene066723:CDS 35.0%
!!! GATCTTTTTTGGGCTATAAG+AGG - chr4.2:21061352-21061371 MS.gene066723:CDS 35.0%
ACGATAACCTGTGGAGTTAT+CGG + chr4.2:21061430-21061449 None:intergenic 40.0%
AGCATTGTTGGAAGAACCAA+AGG - chr4.2:21061759-21061778 MS.gene066723:CDS 40.0%
AGTAAGGAATTCCCCGAATT+AGG - chr4.2:21061652-21061671 MS.gene066723:CDS 40.0%
ATGCAATGGAATCCATATGG+TGG - chr4.2:21061892-21061911 MS.gene066723:CDS 40.0%
CTCGGAGAATTCGAAACATA+AGG + chr4.2:21062072-21062091 None:intergenic 40.0%
CTGACCATACCAAAAAAGAG+TGG + chr4.2:21061719-21061738 None:intergenic 40.0%
CTTATGTTTCGAATTCTCCG+AGG - chr4.2:21062070-21062089 MS.gene066723:CDS 40.0%
GAAATTCTCGGGATCAACTT+TGG + chr4.2:21062229-21062248 None:intergenic 40.0%
GTGAAAAGGAATGTCAGTGA+AGG - chr4.2:21061457-21061476 MS.gene066723:CDS 40.0%
GTGATTGTCTTTCAATGCCT+TGG - chr4.2:21061686-21061705 MS.gene066723:CDS 40.0%
TCTTCCAACAATGCTTCAAG+AGG + chr4.2:21061754-21061773 None:intergenic 40.0%
! TTTTGGTATGGTCAGCCAAT+TGG - chr4.2:21061724-21061743 MS.gene066723:CDS 40.0%
!! GGAGGCATTCCTCAATTATA+GGG - chr4.2:21062092-21062111 MS.gene066723:CDS 40.0%
!! TACTCCTCTTGAAGCATTGT+TGG - chr4.2:21061747-21061766 MS.gene066723:CDS 40.0%
!!! CTTTTTTGGGCTATAAGAGG+TGG - chr4.2:21061355-21061374 MS.gene066723:CDS 40.0%
ACACCGTTTCCTCATAGAGT+AGG - chr4.2:21061943-21061962 MS.gene066723:CDS 45.0%
AGCAACAACACCGAAACCTT+TGG - chr4.2:21060904-21060923 MS.gene066723:CDS 45.0%
CAGCTTAGAATTCGAAGTGG+CGG - chr4.2:21060995-21061014 MS.gene066723:CDS 45.0%
GTTGAATCAGGTGCAACAGT+TGG - chr4.2:21061124-21061143 MS.gene066723:CDS 45.0%
TAATTGAGGAATGCCTCCCT+CGG + chr4.2:21062090-21062109 None:intergenic 45.0%
! AACAAGCTTGCTTTCCCATC+TGG - chr4.2:21061178-21061197 MS.gene066723:CDS 45.0%
! ACAAGCTTGCTTTCCCATCT+GGG - chr4.2:21061179-21061198 MS.gene066723:CDS 45.0%
! ATTGGTTCCGATAACTCCAC+AGG - chr4.2:21061420-21061439 MS.gene066723:CDS 45.0%
! TCAACAGCATGGGTTGAATC+AGG - chr4.2:21061112-21061131 MS.gene066723:CDS 45.0%
! TGAGGAAACGGTGTTTCTGA+TGG + chr4.2:21061937-21061956 None:intergenic 45.0%
! TTTTGGGCTATAAGAGGTGG+TGG - chr4.2:21061358-21061377 MS.gene066723:CDS 45.0%
!! GCTTCAAGAGGAGTACCAAT+TGG + chr4.2:21061742-21061761 None:intergenic 45.0%
!! GGGAGGCATTCCTCAATTAT+AGG - chr4.2:21062091-21062110 MS.gene066723:CDS 45.0%
AGAGTAAAGCAGACCCCAGA+TGG + chr4.2:21061196-21061215 None:intergenic 50.0%
GAGTAAAGCAGACCCCAGAT+GGG + chr4.2:21061195-21061214 None:intergenic 50.0%
GTCTGCTTTACTCTAGGTGC+TGG - chr4.2:21061202-21061221 MS.gene066723:CDS 50.0%
GTGGTCATTTCTCTGGTGGT+GGG - chr4.2:21061224-21061243 MS.gene066723:CDS 50.0%
TGTTTCGAATTCTCCGAGGG+AGG - chr4.2:21062074-21062093 MS.gene066723:CDS 50.0%
! CAAGCTTGCTTTCCCATCTG+GGG - chr4.2:21061180-21061199 MS.gene066723:CDS 50.0%
! TCTGGGGTCTGCTTTACTCT+AGG - chr4.2:21061196-21061215 MS.gene066723:CDS 50.0%
!! CATTTCTCTGGTGGTGGGTA+TGG - chr4.2:21061229-21061248 MS.gene066723:CDS 50.0%
!! TGCTTTACTCTAGGTGCTGG+TGG - chr4.2:21061205-21061224 MS.gene066723:CDS 50.0%
AGTGGCGGTCACGACTTTGA+AGG - chr4.2:21061010-21061029 MS.gene066723:CDS 55.0%
GCTGGTGGTCATTTCTCTGG+TGG - chr4.2:21061220-21061239 MS.gene066723:CDS 55.0%
GGTGGTCATTTCTCTGGTGG+TGG - chr4.2:21061223-21061242 MS.gene066723:CDS 55.0%
! TGGGCTATAAGAGGTGGTGG+TGG - chr4.2:21061361-21061380 MS.gene066723:CDS 55.0%
!! GGTGCTGGTGGTCATTTCTC+TGG - chr4.2:21061217-21061236 MS.gene066723:CDS 55.0%
!!! GGTGGTGGTGGTGCTAGTTT+TGG - chr4.2:21061373-21061392 MS.gene066723:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 21060875 21062299 21060875 ID=MS.gene066723
chr4.2 mRNA 21060875 21062299 21060875 ID=MS.gene066723.t1;Parent=MS.gene066723
chr4.2 exon 21060875 21062299 21060875 ID=MS.gene066723.t1.exon1;Parent=MS.gene066723.t1
chr4.2 CDS 21060875 21062299 21060875 ID=cds.MS.gene066723.t1;Parent=MS.gene066723.t1
Gene Sequence

>MS.gene066723

ATGAAAATACAAAATAAGAGATTTAAAACAGCAACAACACCGAAACCTTTGGCAATTATAACTGCAAAAGATGATTCTCATGTCCAAGAAACAGTTAAATGTGCCAAAAGCAACAACATTCAGCTTAGAATTCGAAGTGGCGGTCACGACTTTGAAGGTTTTTCATATGTATCAGATGTGCCTTTTGTTATACTTGACTTGTTTAATCTCAATTCAGTTGATGTTAATTTACAAGAATCAACAGCATGGGTTGAATCAGGTGCAACAGTTGGTAAGATTTATTATAACATTGCAAAGAAAAGCAACAAGCTTGCTTTCCCATCTGGGGTCTGCTTTACTCTAGGTGCTGGTGGTCATTTCTCTGGTGGTGGGTATGGAAATTTGATGAGAAAATTTGGTCTTTCTGTTGATAATATTATCGATGCAAAAATTGTTGATGTTAAGGGTAATATTCTTGATAGAAAATCAATGGGAGAAGATCTTTTTTGGGCTATAAGAGGTGGTGGTGGTGCTAGTTTTGGTGTTATTCTTTCATGGAAACTTCAATTGGTTCCGATAACTCCACAGGTTATCGTTTTCAATGTGAAAAGGAATGTCAGTGAAGGTGCAACTGATGTCGTTTACAAATGGCAATCAATTGCACCAAAACTGCATAAAGATATATTCATTAGAGTGCAACCTAATGTTGTTCAAATTGGTCAAGAGGGAAAAAAGGTAGTGCAAGTTAGTTTCATTGGTCAATTTTTAGGAAAAGTTGAAAGACTTTTAATTTTGATGAGTAAGGAATTCCCCGAATTAGGTTTGAACCAAAGTGATTGTCTTTCAATGCCTTGGATTAATTCCACTCTTTTTTGGTATGGTCAGCCAATTGGTACTCCTCTTGAAGCATTGTTGGAAGAACCAAAGGATCCTCAACCAATTTATCAAAAAAATAAATCAGATTATGTGAAGAAACCTATTCCTAAAGAAGCTCTAGAATCTATATGGAAATTGATGATTGAAGGTGAGAATTTCCTTATGCAATGGAATCCATATGGTGGAAAGATGAAAGAGATATTGCCATCAGAAACACCGTTTCCTCATAGAGTAGGAAACTTGTTCTTGATTCAATACTTTAATCCGTGGTCTAATGATTCTTCTGAAGTTAGTGAACGTCATGTGAATTTTTCAAGGTCGTTTTATGAATTTATGACGCCTTATGTTTCGAATTCTCCGAGGGAGGCATTCCTCAATTATAGGGATGCTGATATTGGTGCTAATCATCCAAGTAATGTAACGAGATTTGACATTGCTAAAACTTATGGAAGCAAGTTTTTCAAAGGAAATTTTGAAAGACTAGTTAGTGTAAAAACCAAAGTTGATCCCGAGAATTTCTTTAGATATGAACAAAGTATTCCTACTAGATCATTGTATCAGTCATATTGA
Protein sequence

>MS.gene066723.t1

MKIQNKRFKTATTPKPLAIITAKDDSHVQETVKCAKSNNIQLRIRSGGHDFEGFSYVSDVPFVILDLFNLNSVDVNLQESTAWVESGATVGKIYYNIAKKSNKLAFPSGVCFTLGAGGHFSGGGYGNLMRKFGLSVDNIIDAKIVDVKGNILDRKSMGEDLFWAIRGGGGASFGVILSWKLQLVPITPQVIVFNVKRNVSEGATDVVYKWQSIAPKLHKDIFIRVQPNVVQIGQEGKKVVQVSFIGQFLGKVERLLILMSKEFPELGLNQSDCLSMPWINSTLFWYGQPIGTPLEALLEEPKDPQPIYQKNKSDYVKKPIPKEALESIWKLMIEGENFLMQWNPYGGKMKEILPSETPFPHRVGNLFLIQYFNPWSNDSSEVSERHVNFSRSFYEFMTPYVSNSPREAFLNYRDADIGANHPSNVTRFDIAKTYGSKFFKGNFERLVSVKTKVDPENFFRYEQSIPTRSLYQSY