Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052559.t1 | XP_003608243.1 | 93 | 470 | 33 | 0 | 1 | 470 | 59 | 528 | 1.90E-263 | 917.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052559.t1 | Q9FZC5 | 55.9 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 6.8e-153 | 542.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052559.t1 | Q2HTY4 | 93.0 | 470 | 33 | 0 | 1 | 470 | 59 | 528 | 1.4e-263 | 917.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051699 | MS.gene052559 | 0.810229 | 1.28E-50 | -1.69E-46 |
| MS.gene052053 | MS.gene052559 | 0.809386 | 1.94E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene052560 | 0.912229 | 2.47E-83 | -1.69E-46 |
| MS.gene052559 | MS.gene055964 | 0.818504 | 1.89E-52 | -1.69E-46 |
| MS.gene052559 | MS.gene056677 | 0.81073 | 9.97E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene05952 | 0.826133 | 3.21E-54 | -1.69E-46 |
| MS.gene052559 | MS.gene06023 | 0.819024 | 1.44E-52 | -1.69E-46 |
| MS.gene052559 | MS.gene066723 | 0.915385 | 6.27E-85 | -1.69E-46 |
| MS.gene052559 | MS.gene066724 | 0.968379 | 1.36E-128 | -1.69E-46 |
| MS.gene052559 | MS.gene06842 | 0.839204 | 1.82E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene071949 | 0.829505 | 4.96E-55 | -1.69E-46 |
| MS.gene052559 | MS.gene072227 | 0.822581 | 2.19E-53 | -1.69E-46 |
| MS.gene052559 | MS.gene23695 | 0.80862 | 2.83E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene25530 | 0.815225 | 1.03E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene32207 | 0.95706 | 6.73E-115 | -1.69E-46 |
| MS.gene052559 | MS.gene32208 | 0.904936 | 7.28E-80 | -1.69E-46 |
| MS.gene052559 | MS.gene33412 | 0.806053 | 9.91E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene36268 | 0.81324 | 2.83E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene37412 | 0.821592 | 3.72E-53 | -1.69E-46 |
| MS.gene052559 | MS.gene43751 | 0.822498 | 2.29E-53 | -1.69E-46 |
| MS.gene052559 | MS.gene45552 | 0.824797 | 6.65E-54 | -1.69E-46 |
| MS.gene052559 | MS.gene45563 | 0.815425 | 9.31E-52 | -1.69E-46 |
| MS.gene052559 | MS.gene45708 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene052559 | MS.gene46055 | 0.806239 | 9.06E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene49911 | 0.830453 | 2.91E-55 | -1.69E-46 |
| MS.gene052559 | MS.gene55040 | 0.824459 | 7.98E-54 | -1.69E-46 |
| MS.gene052559 | MS.gene55755 | 0.813741 | 2.20E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene56769 | 0.803279 | 3.76E-49 | -1.69E-46 |
| MS.gene052559 | MS.gene57128 | 0.836642 | 8.30E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene62676 | 0.830854 | 2.32E-55 | -1.69E-46 |
| MS.gene052559 | MS.gene62677 | 0.820155 | 7.96E-53 | -1.69E-46 |
| MS.gene052559 | MS.gene65297 | 0.81969 | 1.02E-52 | -1.69E-46 |
| MS.gene052559 | MS.gene65364 | 0.840948 | 6.39E-58 | -1.69E-46 |
| MS.gene052559 | MS.gene65365 | 0.839748 | 1.32E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene69799 | 0.810854 | 9.37E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene73236 | 0.882406 | 1.07E-70 | -1.69E-46 |
| MS.gene052559 | MS.gene73644 | 0.828621 | 8.12E-55 | -1.69E-46 |
| MS.gene052559 | MS.gene79433 | 0.813209 | 2.87E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene79568 | 0.821179 | 4.63E-53 | -1.69E-46 |
| MS.gene052559 | MS.gene81359 | 0.808868 | 2.51E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene89082 | 0.800994 | 1.11E-48 | -1.69E-46 |
| MS.gene052559 | MS.gene89702 | 0.839007 | 2.05E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene90822 | 0.809885 | 1.52E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene92076 | 0.805564 | 1.26E-49 | -1.69E-46 |
| MS.gene052559 | MS.gene97813 | 0.839521 | 1.51E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene97814 | 0.82861 | 8.17E-55 | -1.69E-46 |
| MS.gene052559 | MS.gene97816 | 0.816944 | 4.26E-52 | -1.69E-46 |
| MS.gene052559 | MS.gene98636 | 0.808796 | 2.60E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052559.t1 | MTR_4g091200 | 92.979 | 470 | 33 | 0 | 1 | 470 | 59 | 528 | 0.0 | 896 |
| MS.gene052559.t1 | MTR_6g017205 | 91.453 | 468 | 40 | 0 | 1 | 468 | 59 | 526 | 0.0 | 871 |
| MS.gene052559.t1 | MTR_4g057990 | 82.303 | 469 | 79 | 2 | 1 | 469 | 59 | 523 | 0.0 | 781 |
| MS.gene052559.t1 | MTR_4g091190 | 80.342 | 468 | 90 | 1 | 1 | 468 | 63 | 528 | 0.0 | 768 |
| MS.gene052559.t1 | MTR_4g088355 | 79.104 | 469 | 98 | 0 | 1 | 469 | 66 | 534 | 0.0 | 762 |
| MS.gene052559.t1 | MTR_2g023590 | 80.000 | 470 | 91 | 2 | 1 | 469 | 1 | 468 | 0.0 | 754 |
| MS.gene052559.t1 | MTR_4g091170 | 73.420 | 459 | 104 | 1 | 10 | 468 | 102 | 542 | 0.0 | 710 |
| MS.gene052559.t1 | MTR_4g091150 | 63.675 | 468 | 168 | 2 | 1 | 466 | 71 | 538 | 0.0 | 600 |
| MS.gene052559.t1 | MTR_2g031560 | 54.506 | 466 | 209 | 2 | 3 | 467 | 59 | 522 | 0.0 | 545 |
| MS.gene052559.t1 | MTR_2g031590 | 53.715 | 471 | 209 | 4 | 3 | 467 | 64 | 531 | 3.57e-180 | 515 |
| MS.gene052559.t1 | MTR_4g094498 | 53.763 | 465 | 211 | 3 | 3 | 467 | 73 | 533 | 5.41e-174 | 500 |
| MS.gene052559.t1 | MTR_2g031390 | 50.321 | 467 | 224 | 6 | 4 | 466 | 65 | 527 | 1.89e-166 | 480 |
| MS.gene052559.t1 | MTR_4g094488 | 53.700 | 473 | 210 | 4 | 3 | 468 | 67 | 537 | 2.64e-165 | 478 |
| MS.gene052559.t1 | MTR_4g094495 | 52.331 | 472 | 216 | 4 | 4 | 468 | 71 | 540 | 4.01e-164 | 474 |
| MS.gene052559.t1 | MTR_4g094492 | 50.959 | 469 | 224 | 3 | 4 | 467 | 68 | 535 | 1.28e-163 | 473 |
| MS.gene052559.t1 | MTR_4g094520 | 51.812 | 469 | 215 | 6 | 4 | 467 | 69 | 531 | 2.96e-163 | 472 |
| MS.gene052559.t1 | MTR_4g094480 | 51.599 | 469 | 222 | 3 | 4 | 468 | 68 | 535 | 4.92e-161 | 466 |
| MS.gene052559.t1 | MTR_4g094478 | 52.350 | 468 | 218 | 3 | 4 | 467 | 68 | 534 | 7.86e-161 | 466 |
| MS.gene052559.t1 | MTR_3g009820 | 48.707 | 464 | 231 | 5 | 4 | 466 | 63 | 520 | 9.41e-161 | 465 |
| MS.gene052559.t1 | MTR_4g094518 | 50.533 | 469 | 218 | 7 | 5 | 466 | 67 | 528 | 4.01e-160 | 464 |
| MS.gene052559.t1 | MTR_2g031490 | 51.489 | 470 | 218 | 6 | 3 | 466 | 71 | 536 | 2.34e-159 | 462 |
| MS.gene052559.t1 | MTR_2g031510 | 50.105 | 475 | 217 | 6 | 3 | 466 | 73 | 538 | 5.61e-157 | 456 |
| MS.gene052559.t1 | MTR_2g031420 | 51.282 | 468 | 221 | 4 | 3 | 469 | 67 | 528 | 4.25e-155 | 451 |
| MS.gene052559.t1 | MTR_2g031460 | 50.431 | 464 | 223 | 4 | 3 | 465 | 67 | 524 | 2.82e-154 | 449 |
| MS.gene052559.t1 | MTR_3g009880 | 48.707 | 464 | 231 | 5 | 4 | 466 | 61 | 518 | 9.02e-154 | 447 |
| MS.gene052559.t1 | MTR_3g009760 | 47.414 | 464 | 237 | 5 | 4 | 466 | 63 | 520 | 2.45e-153 | 446 |
| MS.gene052559.t1 | MTR_4g094418 | 51.259 | 437 | 205 | 4 | 40 | 469 | 1 | 436 | 2.50e-153 | 449 |
| MS.gene052559.t1 | MTR_4g094515 | 51.592 | 471 | 219 | 6 | 3 | 468 | 74 | 540 | 4.82e-150 | 457 |
| MS.gene052559.t1 | MTR_4g094515 | 51.709 | 468 | 219 | 5 | 3 | 467 | 686 | 1149 | 2.80e-149 | 455 |
| MS.gene052559.t1 | MTR_1g076830 | 38.559 | 472 | 274 | 8 | 3 | 465 | 68 | 532 | 1.14e-101 | 315 |
| MS.gene052559.t1 | MTR_2g031600 | 65.000 | 180 | 63 | 0 | 3 | 182 | 65 | 244 | 1.86e-71 | 232 |
| MS.gene052559.t1 | MTR_2g031600 | 51.875 | 160 | 76 | 1 | 308 | 467 | 241 | 399 | 6.62e-54 | 186 |
| MS.gene052559.t1 | MTR_3g009850 | 48.214 | 224 | 113 | 3 | 244 | 466 | 5 | 226 | 3.64e-64 | 207 |
| MS.gene052559.t1 | MTR_2g084370 | 46.847 | 111 | 37 | 2 | 64 | 174 | 1 | 89 | 5.49e-29 | 110 |
| MS.gene052559.t1 | MTR_2g084405 | 45.570 | 79 | 43 | 0 | 64 | 142 | 1 | 79 | 4.98e-16 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052559.t1 | AT4G20800 | 55.579 | 466 | 204 | 2 | 4 | 469 | 64 | 526 | 1.16e-178 | 511 |
| MS.gene052559.t1 | AT1G26420 | 55.172 | 464 | 202 | 4 | 4 | 466 | 65 | 523 | 1.36e-176 | 506 |
| MS.gene052559.t1 | AT1G26390 | 55.914 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 3.00e-175 | 502 |
| MS.gene052559.t1 | AT1G26380 | 55.388 | 464 | 201 | 4 | 4 | 466 | 65 | 523 | 6.54e-175 | 501 |
| MS.gene052559.t1 | AT1G30720 | 53.632 | 468 | 212 | 3 | 2 | 468 | 63 | 526 | 5.01e-173 | 496 |
| MS.gene052559.t1 | AT1G34575 | 55.342 | 468 | 195 | 6 | 3 | 466 | 65 | 522 | 1.29e-172 | 496 |
| MS.gene052559.t1 | AT1G30730 | 52.350 | 468 | 218 | 3 | 2 | 468 | 62 | 525 | 1.72e-170 | 490 |
| MS.gene052559.t1 | AT1G26400 | 53.632 | 468 | 211 | 6 | 4 | 470 | 65 | 527 | 1.82e-170 | 490 |
| MS.gene052559.t1 | AT1G30710 | 54.449 | 472 | 200 | 7 | 3 | 469 | 68 | 529 | 2.79e-170 | 490 |
| MS.gene052559.t1 | AT1G26410 | 55.172 | 464 | 202 | 5 | 4 | 466 | 89 | 547 | 4.53e-169 | 488 |
| MS.gene052559.t1 | AT1G30700 | 52.350 | 468 | 214 | 5 | 3 | 466 | 62 | 524 | 4.62e-165 | 476 |
| MS.gene052559.t1 | AT5G44400 | 50.107 | 467 | 225 | 6 | 4 | 466 | 72 | 534 | 5.64e-160 | 464 |
| MS.gene052559.t1 | AT5G44390 | 50.746 | 469 | 223 | 5 | 4 | 467 | 74 | 539 | 1.31e-158 | 460 |
| MS.gene052559.t1 | AT2G34790 | 50.000 | 468 | 224 | 5 | 4 | 468 | 70 | 530 | 6.39e-158 | 458 |
| MS.gene052559.t1 | AT2G34810 | 49.787 | 470 | 230 | 3 | 3 | 467 | 70 | 538 | 2.28e-157 | 457 |
| MS.gene052559.t1 | AT4G20840 | 51.277 | 470 | 216 | 7 | 3 | 467 | 73 | 534 | 4.34e-157 | 456 |
| MS.gene052559.t1 | AT1G30760 | 50.000 | 468 | 222 | 6 | 4 | 467 | 72 | 531 | 1.38e-154 | 450 |
| MS.gene052559.t1 | AT1G30760 | 50.000 | 468 | 222 | 6 | 4 | 467 | 90 | 549 | 1.99e-154 | 450 |
| MS.gene052559.t1 | AT1G11770 | 49.254 | 469 | 227 | 6 | 3 | 467 | 67 | 528 | 3.30e-151 | 441 |
| MS.gene052559.t1 | AT1G01980 | 51.158 | 475 | 214 | 9 | 3 | 467 | 67 | 533 | 2.69e-148 | 434 |
| MS.gene052559.t1 | AT5G44380 | 48.712 | 466 | 233 | 4 | 4 | 466 | 75 | 537 | 7.78e-147 | 430 |
| MS.gene052559.t1 | AT5G44380 | 48.712 | 466 | 233 | 4 | 4 | 466 | 115 | 577 | 1.42e-146 | 431 |
| MS.gene052559.t1 | AT4G20860 | 48.026 | 456 | 231 | 4 | 12 | 466 | 77 | 527 | 2.39e-146 | 429 |
| MS.gene052559.t1 | AT4G20820 | 47.854 | 466 | 229 | 5 | 3 | 466 | 71 | 524 | 8.52e-146 | 427 |
| MS.gene052559.t1 | AT5G44360 | 48.394 | 467 | 229 | 7 | 4 | 466 | 70 | 528 | 1.45e-142 | 419 |
| MS.gene052559.t1 | AT5G44360 | 48.394 | 467 | 229 | 7 | 4 | 466 | 86 | 544 | 1.62e-142 | 419 |
| MS.gene052559.t1 | AT4G20830 | 49.574 | 470 | 224 | 7 | 3 | 467 | 74 | 535 | 9.70e-142 | 417 |
| MS.gene052559.t1 | AT4G20830 | 49.574 | 470 | 224 | 7 | 3 | 467 | 74 | 535 | 4.09e-141 | 417 |
| MS.gene052559.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 65 | 527 | 3.41e-139 | 410 |
| MS.gene052559.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 135 | 597 | 8.60e-138 | 409 |
| MS.gene052559.t1 | AT5G44410 | 48.832 | 471 | 222 | 9 | 3 | 466 | 69 | 527 | 3.19e-136 | 403 |
| MS.gene052559.t1 | AT1G30740 | 47.872 | 470 | 234 | 7 | 3 | 467 | 64 | 527 | 3.20e-132 | 393 |
| MS.gene052559.t1 | AT1G30740 | 47.872 | 470 | 234 | 7 | 3 | 467 | 67 | 530 | 4.14e-132 | 392 |
Find 85 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCTAATGTTGTTCAAAT+TGG | 0.228780 | 4.3:-22832666 | MS.gene052559:CDS |
| AAGCTGAATGTTGTTGCTTT+TGG | 0.230709 | 4.3:+22833238 | None:intergenic |
| GGAAATTTGATGAGAAAATT+TGG | 0.258916 | 4.3:-22832966 | MS.gene052559:CDS |
| TGGCACATTTAACTGTTTCT+TGG | 0.267359 | 4.3:+22833258 | None:intergenic |
| AAACTTGCTTCCATATGTTT+TGG | 0.269184 | 4.3:+22832050 | None:intergenic |
| AATAGATCTTTATGCAGTTT+TGG | 0.273624 | 4.3:+22832699 | None:intergenic |
| AATCATTAGACCACGAATTA+AGG | 0.276835 | 4.3:+22832229 | None:intergenic |
| AGTAAGGAATTCCCCGAATT+AGG | 0.285169 | 4.3:-22832564 | MS.gene052559:CDS |
| AACAAGCTTGCTTTCCCATC+TGG | 0.299204 | 4.3:-22833038 | MS.gene052559:CDS |
| GGGAGGCATTTCTCAATTAT+AGG | 0.301839 | 4.3:-22832125 | MS.gene052559:CDS |
| ACTTTGGTTCAAACCTAATT+CGG | 0.311377 | 4.3:+22832551 | None:intergenic |
| TGACCAATTTGAACAACATT+AGG | 0.312133 | 4.3:+22832663 | None:intergenic |
| TCTTTCATCTTTCCACCATA+TGG | 0.316468 | 4.3:+22832312 | None:intergenic |
| ATAGATTCTAGAGCTTCTTT+AGG | 0.318699 | 4.3:+22832381 | None:intergenic |
| TCTAGAGCTTCTTTAGGAAT+AGG | 0.321024 | 4.3:+22832387 | None:intergenic |
| GGTGGTGGTGGTGCTAGTTT+TGG | 0.331271 | 4.3:-22832843 | MS.gene052559:CDS |
| AAGAAGCTCTAGAATCTATA+TGG | 0.334429 | 4.3:-22832377 | MS.gene052559:CDS |
| TTTGACATTGCCAAAACATA+TGG | 0.339181 | 4.3:-22832060 | MS.gene052559:CDS |
| CTTTGGTTCAAACCTAATTC+GGG | 0.343696 | 4.3:+22832552 | None:intergenic |
| CATTTCTCTGGTGGTGGGTA+TGG | 0.360817 | 4.3:-22832987 | MS.gene052559:CDS |
| GTTATAATCGCCAAAGGTTT+CGG | 0.368910 | 4.3:+22833302 | None:intergenic |
| AATTATAGGGATGCTGATAT+TGG | 0.376157 | 4.3:-22832111 | MS.gene052559:CDS |
| GTAGTGCAAGTTAGTTTCAT+TGG | 0.379275 | 4.3:-22832627 | MS.gene052559:CDS |
| GGAGGCATTTCTCAATTATA+GGG | 0.389544 | 4.3:-22832124 | MS.gene052559:CDS |
| GTGATTGCCTTTCAATGCCT+TGG | 0.391339 | 4.3:-22832530 | MS.gene052559:CDS |
| TCAAATCTCGTTACATTACT+TGG | 0.393941 | 4.3:+22832078 | None:intergenic |
| ACGATAACCTGTGGAGTTAT+CGG | 0.397449 | 4.3:+22832789 | None:intergenic |
| TAAATTGGTTGAGGATCCTT+TGG | 0.399190 | 4.3:+22832441 | None:intergenic |
| TACTCCTCTTGAAGCATTGT+TGG | 0.402290 | 4.3:-22832469 | MS.gene052559:CDS |
| GGTGGTCATTTCTCTGGTGG+TGG | 0.426644 | 4.3:-22832993 | MS.gene052559:CDS |
| TCAACAGCATGGGTTGAATC+AGG | 0.427451 | 4.3:-22833104 | MS.gene052559:CDS |
| AACAAGTCAAGTATAACAAA+AGG | 0.433036 | 4.3:+22833161 | None:intergenic |
| ACAAGCTTGCTTTCCCATCT+GGG | 0.436569 | 4.3:-22833037 | MS.gene052559:CDS |
| TCTTTCATGGAAACTTCAAT+TGG | 0.441584 | 4.3:-22832814 | MS.gene052559:CDS |
| GAAATTCTCAGGATCAACTT+TGG | 0.444017 | 4.3:+22831990 | None:intergenic |
| CTTATGCAATGGAATCCATA+TGG | 0.450449 | 4.3:-22832327 | MS.gene052559:CDS |
| TATTCTTGATAGAAAATCAA+TGG | 0.458066 | 4.3:-22832892 | MS.gene052559:CDS |
| GGTGCTGGTGGTCATTTCTC+TGG | 0.459504 | 4.3:-22832999 | MS.gene052559:CDS |
| TCTGGGGTCTGCTTTACTCT+AGG | 0.464774 | 4.3:-22833020 | MS.gene052559:CDS |
| AGCAACAACACCGAAACCTT+TGG | 0.466175 | 4.3:-22833312 | MS.gene052559:CDS |
| CTCGGAGAATTCGAAACATA+AGG | 0.468696 | 4.3:+22832147 | None:intergenic |
| GTCTGCTTTACTCTAGGTGC+TGG | 0.472600 | 4.3:-22833014 | MS.gene052559:CDS |
| CATATGGATTCCATTGCATA+AGG | 0.477148 | 4.3:+22832328 | None:intergenic |
| TGATTCAATACCTTAATTCG+TGG | 0.477340 | 4.3:-22832239 | MS.gene052559:CDS |
| CAACTGATGTTGTTTACAAA+TGG | 0.485694 | 4.3:-22832734 | MS.gene052559:CDS |
| TCATATCTAAAGAAATTCTC+AGG | 0.490816 | 4.3:+22831979 | None:intergenic |
| GTGGTCATTTCTCTGGTGGT+GGG | 0.501321 | 4.3:-22832992 | MS.gene052559:CDS |
| AGAGTAAAGCAGACCCCAGA+TGG | 0.503056 | 4.3:+22833023 | None:intergenic |
| AACAAGTTTCCTACTCTATG+AGG | 0.510558 | 4.3:+22832264 | None:intergenic |
| TGCTTTACTCTAGGTGCTGG+TGG | 0.512366 | 4.3:-22833011 | MS.gene052559:CDS |
| GCATTGAAAGGCAATCACTT+TGG | 0.516798 | 4.3:+22832535 | None:intergenic |
| TGGGCTATAAGAGGTGGTGG+TGG | 0.518497 | 4.3:-22832855 | MS.gene052559:CDS |
| TTTCCTACTCTATGAGGAAA+CGG | 0.519543 | 4.3:+22832270 | None:intergenic |
| GCTTCAAGAGGAGTACCAAT+TGG | 0.520632 | 4.3:+22832477 | None:intergenic |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 4.3:-22832891 | MS.gene052559:CDS |
| GTGAGAATTTCCTTATGCAA+TGG | 0.526102 | 4.3:-22832338 | MS.gene052559:CDS |
| GTTGTTCAAATTGGTCAAGA+GGG | 0.530168 | 4.3:-22832657 | MS.gene052559:CDS |
| ATTGGTTCCGATAACTCCAC+AGG | 0.531147 | 4.3:-22832796 | MS.gene052559:CDS |
| ATATGGAAATTGATGATTGA+AGG | 0.537491 | 4.3:-22832360 | MS.gene052559:CDS |
| ACAAGTCAAGTATAACAAAA+GGG | 0.545712 | 4.3:+22833162 | None:intergenic |
| GTTGAATCAGGTGCAACACT+TGG | 0.555249 | 4.3:-22833092 | MS.gene052559:CDS |
| TGTTGTTCAAATTGGTCAAG+AGG | 0.558808 | 4.3:-22832658 | MS.gene052559:CDS |
| AGCATTGTTGGAAGAACCAA+AGG | 0.559301 | 4.3:-22832457 | MS.gene052559:CDS |
| GCTGGTGGTCATTTCTCTGG+TGG | 0.564457 | 4.3:-22832996 | MS.gene052559:CDS |
| CAAGCTTGCTTTCCCATCTG+GGG | 0.567506 | 4.3:-22833036 | MS.gene052559:CDS |
| AATTAATCCAAGGCATTGAA+AGG | 0.570542 | 4.3:+22832523 | None:intergenic |
| AGTGGCGGTCACGACTATGA+AGG | 0.571849 | 4.3:-22833206 | MS.gene052559:CDS |
| TTTACAAGAATCAACAGCAT+GGG | 0.572516 | 4.3:-22833114 | MS.gene052559:CDS |
| TGAGGAAACGGTGTTTCTGA+TGG | 0.577426 | 4.3:+22832282 | None:intergenic |
| TTTGCAGTTATAATCGCCAA+AGG | 0.586317 | 4.3:+22833296 | None:intergenic |
| TAATTGAGAAATGCCTCCCT+CGG | 0.594640 | 4.3:+22832129 | None:intergenic |
| TCTTCCAACAATGCTTCAAG+AGG | 0.594711 | 4.3:+22832465 | None:intergenic |
| ACACCGTTTCCTCATAGAGT+AGG | 0.600052 | 4.3:-22832273 | MS.gene052559:CDS |
| AAAAGAGTGGAATTAATCCA+AGG | 0.600902 | 4.3:+22832513 | None:intergenic |
| TTATGTTTCGAATTCTCCGA+GGG | 0.609571 | 4.3:-22832145 | MS.gene052559:CDS |
| ATTTACAAGAATCAACAGCA+TGG | 0.616536 | 4.3:-22833115 | MS.gene052559:CDS |
| TGTTTCGAATTCTCCGAGGG+AGG | 0.634404 | 4.3:-22832142 | MS.gene052559:CDS |
| GAGTAAAGCAGACCCCAGAT+GGG | 0.637967 | 4.3:+22833024 | None:intergenic |
| CAGCTTAGAATTCGAAGTGG+CGG | 0.641139 | 4.3:-22833221 | MS.gene052559:CDS |
| TTTGGTTCAAACCTAATTCG+GGG | 0.645523 | 4.3:+22832553 | None:intergenic |
| ATGCAATGGAATCCATATGG+TGG | 0.646403 | 4.3:-22832324 | MS.gene052559:CDS |
| ACATCGAAAACGATAACCTG+TGG | 0.672224 | 4.3:+22832780 | None:intergenic |
| CTTATGTTTCGAATTCTCCG+AGG | 0.672859 | 4.3:-22832146 | MS.gene052559:CDS |
| ATTCAGCTTAGAATTCGAAG+TGG | 0.701549 | 4.3:-22833224 | MS.gene052559:CDS |
| GTGAAAAGGAATGTCAGTGA+AGG | 0.713631 | 4.3:-22832759 | MS.gene052559:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTATTTTTTTGATAAAT+TGG | + | chr4.3:22832869-22832888 | None:intergenic | 10.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | - | chr4.3:22832401-22832420 | MS.gene052559:CDS | 20.0% |
| !! | TATTCTTGATAGAAAATCAA+TGG | - | chr4.3:22832400-22832419 | MS.gene052559:CDS | 20.0% |
| !!! | ACTTTTAATTTTGATGAGTA+AGG | - | chr4.3:22832712-22832731 | MS.gene052559:CDS | 20.0% |
| !!! | TTTTTTTGATAAATTGGTTG+AGG | + | chr4.3:22832863-22832882 | None:intergenic | 20.0% |
| ! | AACAAGTCAAGTATAACAAA+AGG | + | chr4.3:22832134-22832153 | None:intergenic | 25.0% |
| ! | ACAAGTCAAGTATAACAAAA+GGG | + | chr4.3:22832133-22832152 | None:intergenic | 25.0% |
| ! | GCAAAAATTGTTGATGTTAA+GGG | - | chr4.3:22832374-22832393 | MS.gene052559:CDS | 25.0% |
| ! | GGAAATTTGATGAGAAAATT+TGG | - | chr4.3:22832326-22832345 | MS.gene052559:CDS | 25.0% |
| ! | TCATATCTAAAGAAATTCTC+AGG | + | chr4.3:22833316-22833335 | None:intergenic | 25.0% |
| ! | TGCAAAAATTGTTGATGTTA+AGG | - | chr4.3:22832373-22832392 | MS.gene052559:CDS | 25.0% |
| !! | AATAGATCTTTATGCAGTTT+TGG | + | chr4.3:22832596-22832615 | None:intergenic | 25.0% |
| !! | AGTTTCATTGGTCAATTTTT+AGG | - | chr4.3:22832677-22832696 | MS.gene052559:CDS | 25.0% |
| !! | ATATGGAAATTGATGATTGA+AGG | - | chr4.3:22832932-22832951 | MS.gene052559:CDS | 25.0% |
| AAAAGAGTGGAATTAATCCA+AGG | + | chr4.3:22832782-22832801 | None:intergenic | 30.0% | |
| AATCATTAGACCACGAATTA+AGG | + | chr4.3:22833066-22833085 | None:intergenic | 30.0% | |
| AATTATAGGGATGCTGATAT+TGG | - | chr4.3:22833181-22833200 | MS.gene052559:CDS | 30.0% | |
| ATTTACAAGAATCAACAGCA+TGG | - | chr4.3:22832177-22832196 | MS.gene052559:CDS | 30.0% | |
| CAACCTAATGTTGTTCAAAT+TGG | - | chr4.3:22832626-22832645 | MS.gene052559:CDS | 30.0% | |
| TCAAATCTCGTTACATTACT+TGG | + | chr4.3:22833217-22833236 | None:intergenic | 30.0% | |
| TCTTTCATGGAAACTTCAAT+TGG | - | chr4.3:22832478-22832497 | MS.gene052559:CDS | 30.0% | |
| TGACCAATTTGAACAACATT+AGG | + | chr4.3:22832632-22832651 | None:intergenic | 30.0% | |
| TGATTCAATACCTTAATTCG+TGG | - | chr4.3:22833053-22833072 | MS.gene052559:CDS | 30.0% | |
| TTTACAAGAATCAACAGCAT+GGG | - | chr4.3:22832178-22832197 | MS.gene052559:CDS | 30.0% | |
| TTTGACATTGCCAAAACATA+TGG | - | chr4.3:22833232-22833251 | MS.gene052559:CDS | 30.0% | |
| ! | ACTTTGGTTCAAACCTAATT+CGG | + | chr4.3:22832744-22832763 | None:intergenic | 30.0% |
| ! | CAACTGATGTTGTTTACAAA+TGG | - | chr4.3:22832558-22832577 | MS.gene052559:CDS | 30.0% |
| ! | TTATCGTTTTCGATGTGAAA+AGG | - | chr4.3:22832519-22832538 | MS.gene052559:CDS | 30.0% |
| !! | AAACTTGCTTCCATATGTTT+TGG | + | chr4.3:22833245-22833264 | None:intergenic | 30.0% |
| !! | AAGAAGCTCTAGAATCTATA+TGG | - | chr4.3:22832915-22832934 | MS.gene052559:CDS | 30.0% |
| !! | AATTAATCCAAGGCATTGAA+AGG | + | chr4.3:22832772-22832791 | None:intergenic | 30.0% |
| !! | ATAGATTCTAGAGCTTCTTT+AGG | + | chr4.3:22832914-22832933 | None:intergenic | 30.0% |
| !! | TATGGAAGCAAGTTTTTCAA+AGG | - | chr4.3:22833250-22833269 | MS.gene052559:CDS | 30.0% |
| !!! | AATGGGAGAAGATCTTTTTT+GGG | - | chr4.3:22832418-22832437 | MS.gene052559:CDS | 30.0% |
| !!! | AATTCCACTCTTTTTTGGTA+TGG | - | chr4.3:22832788-22832807 | MS.gene052559:CDS | 30.0% |
| !!! | GGATTAATTCCACTCTTTTT+TGG | - | chr4.3:22832783-22832802 | MS.gene052559:CDS | 30.0% |
| !!! | GTTTTGGTGTTATTCTTTCA+TGG | - | chr4.3:22832465-22832484 | MS.gene052559:CDS | 30.0% |
| AACAAGTTTCCTACTCTATG+AGG | + | chr4.3:22833031-22833050 | None:intergenic | 35.0% | |
| AATTGGTCAAGAGGGAAAAA+AGG | - | chr4.3:22832643-22832662 | MS.gene052559:CDS | 35.0% | |
| ATTCAGCTTAGAATTCGAAG+TGG | - | chr4.3:22832068-22832087 | MS.gene052559:CDS | 35.0% | |
| CATATGGATTCCATTGCATA+AGG | + | chr4.3:22832967-22832986 | None:intergenic | 35.0% | |
| CTTATGCAATGGAATCCATA+TGG | - | chr4.3:22832965-22832984 | MS.gene052559:CDS | 35.0% | |
| GAAATTCTCAGGATCAACTT+TGG | + | chr4.3:22833305-22833324 | None:intergenic | 35.0% | |
| GTGAGAATTTCCTTATGCAA+TGG | - | chr4.3:22832954-22832973 | MS.gene052559:CDS | 35.0% | |
| GTTATAATCGCCAAAGGTTT+CGG | + | chr4.3:22831993-22832012 | None:intergenic | 35.0% | |
| GTTGTTCAAATTGGTCAAGA+GGG | - | chr4.3:22832635-22832654 | MS.gene052559:CDS | 35.0% | |
| TAAATTGGTTGAGGATCCTT+TGG | + | chr4.3:22832854-22832873 | None:intergenic | 35.0% | |
| TCTTTCATCTTTCCACCATA+TGG | + | chr4.3:22832983-22833002 | None:intergenic | 35.0% | |
| TGTTGTTCAAATTGGTCAAG+AGG | - | chr4.3:22832634-22832653 | MS.gene052559:CDS | 35.0% | |
| TTATGTTTCGAATTCTCCGA+GGG | - | chr4.3:22833147-22833166 | MS.gene052559:CDS | 35.0% | |
| TTTCCTACTCTATGAGGAAA+CGG | + | chr4.3:22833025-22833044 | None:intergenic | 35.0% | |
| TTTGCAGTTATAATCGCCAA+AGG | + | chr4.3:22831999-22832018 | None:intergenic | 35.0% | |
| ! | CTTTGGTTCAAACCTAATTC+GGG | + | chr4.3:22832743-22832762 | None:intergenic | 35.0% |
| ! | GTAGTGCAAGTTAGTTTCAT+TGG | - | chr4.3:22832665-22832684 | MS.gene052559:CDS | 35.0% |
| ! | TCTAGAGCTTCTTTAGGAAT+AGG | + | chr4.3:22832908-22832927 | None:intergenic | 35.0% |
| ! | TGGCACATTTAACTGTTTCT+TGG | + | chr4.3:22832037-22832056 | None:intergenic | 35.0% |
| ! | TTTGGTTCAAACCTAATTCG+GGG | + | chr4.3:22832742-22832761 | None:intergenic | 35.0% |
| !! | GGAGGCATTTCTCAATTATA+GGG | - | chr4.3:22833168-22833187 | MS.gene052559:CDS | 35.0% |
| !!! | AAGCTGAATGTTGTTGCTTT+TGG | + | chr4.3:22832057-22832076 | None:intergenic | 35.0% |
| !!! | CAATGGGAGAAGATCTTTTT+TGG | - | chr4.3:22832417-22832436 | MS.gene052559:CDS | 35.0% |
| !!! | CTTGTTGCTTTTGTTTGCAA+TGG | + | chr4.3:22832240-22832259 | None:intergenic | 35.0% |
| !!! | GATCTTTTTTGGGCTATAAG+AGG | - | chr4.3:22832428-22832447 | MS.gene052559:CDS | 35.0% |
| ACATCGAAAACGATAACCTG+TGG | + | chr4.3:22832515-22832534 | None:intergenic | 40.0% | |
| ACGATAACCTGTGGAGTTAT+CGG | + | chr4.3:22832506-22832525 | None:intergenic | 40.0% | |
| AGCATTGTTGGAAGAACCAA+AGG | - | chr4.3:22832835-22832854 | MS.gene052559:CDS | 40.0% | |
| AGTAAGGAATTCCCCGAATT+AGG | - | chr4.3:22832728-22832747 | MS.gene052559:CDS | 40.0% | |
| ATGCAATGGAATCCATATGG+TGG | - | chr4.3:22832968-22832987 | MS.gene052559:CDS | 40.0% | |
| CTCGGAGAATTCGAAACATA+AGG | + | chr4.3:22833148-22833167 | None:intergenic | 40.0% | |
| CTGACCATACCAAAAAAGAG+TGG | + | chr4.3:22832795-22832814 | None:intergenic | 40.0% | |
| CTTATGTTTCGAATTCTCCG+AGG | - | chr4.3:22833146-22833165 | MS.gene052559:CDS | 40.0% | |
| GCATTGAAAGGCAATCACTT+TGG | + | chr4.3:22832760-22832779 | None:intergenic | 40.0% | |
| GTGAAAAGGAATGTCAGTGA+AGG | - | chr4.3:22832533-22832552 | MS.gene052559:CDS | 40.0% | |
| TAATTGAGAAATGCCTCCCT+CGG | + | chr4.3:22833166-22833185 | None:intergenic | 40.0% | |
| TCTTCCAACAATGCTTCAAG+AGG | + | chr4.3:22832830-22832849 | None:intergenic | 40.0% | |
| ! | TTTTGGTATGGTCAGCCAAT+TGG | - | chr4.3:22832800-22832819 | MS.gene052559:CDS | 40.0% |
| !! | GGGAGGCATTTCTCAATTAT+AGG | - | chr4.3:22833167-22833186 | MS.gene052559:CDS | 40.0% |
| !! | TACTCCTCTTGAAGCATTGT+TGG | - | chr4.3:22832823-22832842 | MS.gene052559:CDS | 40.0% |
| !!! | CTTTTTTGGGCTATAAGAGG+TGG | - | chr4.3:22832431-22832450 | MS.gene052559:CDS | 40.0% |
| ACACCGTTTCCTCATAGAGT+AGG | - | chr4.3:22833019-22833038 | MS.gene052559:CDS | 45.0% | |
| AGCAACAACACCGAAACCTT+TGG | - | chr4.3:22831980-22831999 | MS.gene052559:CDS | 45.0% | |
| CAGCTTAGAATTCGAAGTGG+CGG | - | chr4.3:22832071-22832090 | MS.gene052559:CDS | 45.0% | |
| GTGATTGCCTTTCAATGCCT+TGG | - | chr4.3:22832762-22832781 | MS.gene052559:CDS | 45.0% | |
| GTTGAATCAGGTGCAACACT+TGG | - | chr4.3:22832200-22832219 | MS.gene052559:CDS | 45.0% | |
| ! | AACAAGCTTGCTTTCCCATC+TGG | - | chr4.3:22832254-22832273 | MS.gene052559:CDS | 45.0% |
| ! | ACAAGCTTGCTTTCCCATCT+GGG | - | chr4.3:22832255-22832274 | MS.gene052559:CDS | 45.0% |
| ! | ATTGGTTCCGATAACTCCAC+AGG | - | chr4.3:22832496-22832515 | MS.gene052559:CDS | 45.0% |
| ! | TCAACAGCATGGGTTGAATC+AGG | - | chr4.3:22832188-22832207 | MS.gene052559:CDS | 45.0% |
| ! | TGAGGAAACGGTGTTTCTGA+TGG | + | chr4.3:22833013-22833032 | None:intergenic | 45.0% |
| ! | TTTTGGGCTATAAGAGGTGG+TGG | - | chr4.3:22832434-22832453 | MS.gene052559:CDS | 45.0% |
| !! | GCTTCAAGAGGAGTACCAAT+TGG | + | chr4.3:22832818-22832837 | None:intergenic | 45.0% |
| AGAGTAAAGCAGACCCCAGA+TGG | + | chr4.3:22832272-22832291 | None:intergenic | 50.0% | |
| GAGTAAAGCAGACCCCAGAT+GGG | + | chr4.3:22832271-22832290 | None:intergenic | 50.0% | |
| GTCTGCTTTACTCTAGGTGC+TGG | - | chr4.3:22832278-22832297 | MS.gene052559:CDS | 50.0% | |
| GTGGTCATTTCTCTGGTGGT+GGG | - | chr4.3:22832300-22832319 | MS.gene052559:CDS | 50.0% | |
| TGTTTCGAATTCTCCGAGGG+AGG | - | chr4.3:22833150-22833169 | MS.gene052559:CDS | 50.0% | |
| ! | CAAGCTTGCTTTCCCATCTG+GGG | - | chr4.3:22832256-22832275 | MS.gene052559:CDS | 50.0% |
| ! | TCTGGGGTCTGCTTTACTCT+AGG | - | chr4.3:22832272-22832291 | MS.gene052559:CDS | 50.0% |
| !! | CATTTCTCTGGTGGTGGGTA+TGG | - | chr4.3:22832305-22832324 | MS.gene052559:CDS | 50.0% |
| !! | TGCTTTACTCTAGGTGCTGG+TGG | - | chr4.3:22832281-22832300 | MS.gene052559:CDS | 50.0% |
| AGTGGCGGTCACGACTATGA+AGG | - | chr4.3:22832086-22832105 | MS.gene052559:CDS | 55.0% | |
| GCTGGTGGTCATTTCTCTGG+TGG | - | chr4.3:22832296-22832315 | MS.gene052559:CDS | 55.0% | |
| GGTGGTCATTTCTCTGGTGG+TGG | - | chr4.3:22832299-22832318 | MS.gene052559:CDS | 55.0% | |
| ! | TGGGCTATAAGAGGTGGTGG+TGG | - | chr4.3:22832437-22832456 | MS.gene052559:CDS | 55.0% |
| !! | GGTGCTGGTGGTCATTTCTC+TGG | - | chr4.3:22832293-22832312 | MS.gene052559:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGCTAGTTT+TGG | - | chr4.3:22832449-22832468 | MS.gene052559:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 22831951 | 22833363 | 22831951 | ID=MS.gene052559 |
| chr4.3 | mRNA | 22831951 | 22833363 | 22831951 | ID=MS.gene052559.t1;Parent=MS.gene052559 |
| chr4.3 | exon | 22831951 | 22833363 | 22831951 | ID=MS.gene052559.t1.exon1;Parent=MS.gene052559.t1 |
| chr4.3 | CDS | 22831951 | 22833363 | 22831951 | ID=cds.MS.gene052559.t1;Parent=MS.gene052559.t1 |
| Gene Sequence |
| Protein sequence |
