AlfalfaGEDB | MS.gene066812

M. sativa cultivar XinJiangDaYe / MS.gene066812

KEGG: K12946
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005787 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0032991 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0098796 GO:0110165 GO:0140096 GO:1901564 GO:1902494 GO:1905368
PFAM: PF06645.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066812.t1	XP_003601799.1	98.9	92	1	0	1	92	1	92	1.30E-43	185.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066812.t1	Q944J0	71.6	88	25	0	1	88	1	88	6.4e-31	134.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066812.t1	G7JC07	98.9	92	1	0	1	92	1	92	9.4e-44	185.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene066812	MS.gene89036	PPI
MS.gene066812	MS.gene36215	PPI
MS.gene066812	MS.gene86381	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066812.t1	MTR_3g085510	98.913	92	1	0	1	92	1	92	1.05e-63	187
MS.gene066812.t1	MTR_8g080380	78.261	92	20	0	1	92	92	183	1.89e-51	159

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066812.t1	AT4G40042	79.747	79	16	1	79	1	79	1.35e-43	137
MS.gene066812.t1	AT2G22425	71.591	88	25	1	88	1	88	1.89e-41	131
MS.gene066812.t1	AT2G22425	71.591	88	25	1	88	1	88	1.89e-41	131

Find 22 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAGGATGGCGGTTGAAAA+AGG	0.272451	3.4:+79801482	None:intergenic
CATTGGTGACTGTCCCAAAT+TGG	0.297025	3.4:-79801506	MS.gene066812:CDS
GCATTTGCTGTGGTTGCATT+TGG	0.308000	3.4:-79801597	MS.gene066812:CDS
TTCTTCTTTGAATTCACAAT+AGG	0.417935	3.4:+79801408	None:intergenic
TCTGCATTAGCTTCTCTGCT+AGG	0.440951	3.4:+79801631	None:intergenic
ATAGGAGATAGCTGTGGCTT+CGG	0.450784	3.4:+79801426	None:intergenic
GCTGTGGTTGCATTTGGAAC+GGG	0.459654	3.4:-79801591	MS.gene066812:CDS
TGCTGTGGTTGCATTTGGAA+CGG	0.510809	3.4:-79801592	MS.gene066812:CDS
TTCATCAGATGGATTGGCAA+GGG	0.536217	3.4:-79801659	None:intergenic
CCGCCATCCTCTCAAGTGGT+TGG	0.536748	3.4:-79801472	MS.gene066812:CDS
TCAACCGCCATCCTCTCAAG+TGG	0.553865	3.4:-79801476	MS.gene066812:CDS
GATCCAACCACTTGAGAGGA+TGG	0.566183	3.4:+79801469	None:intergenic
CTTCATCAGATGGATTGGCA+AGG	0.566563	3.4:-79801660	None:intergenic
CTTGGATCCAACCACTTGAG+AGG	0.567895	3.4:+79801465	None:intergenic
AATGTTGCTTGCATTTGCTG+TGG	0.601891	3.4:-79801607	MS.gene066812:CDS
ACGATGATGCTCATATATGC+TGG	0.624836	3.4:-79801546	MS.gene066812:CDS
TGGTGTTGTTCTCACCACAT+TGG	0.662379	3.4:-79801523	MS.gene066812:CDS
CAAGTGGTTGGATCCAAGTG+AGG	0.664517	3.4:-79801460	MS.gene066812:CDS
ATGATGCTCATATATGCTGG+TGG	0.699379	3.4:-79801543	MS.gene066812:CDS
CCAACCACTTGAGAGGATGG+CGG	0.730520	3.4:+79801472	None:intergenic
ATTTGGGACAGTCACCAATG+TGG	0.730637	3.4:+79801509	None:intergenic
TTCACAATAGGAGATAGCTG+TGG	0.732645	3.4:+79801420	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCTTCTTTGAATTCACAAT+AGG	+	chr3.4:79801642-79801661	None:intergenic	25.0%
	ACGATGATGCTCATATATGC+TGG	-	chr3.4:79801501-79801520	MS.gene066812:CDS	40.0%
	ATGATGCTCATATATGCTGG+TGG	-	chr3.4:79801504-79801523	MS.gene066812:CDS	40.0%
	GGTTGAAAAAGGGCCAATTT+GGG	+	chr3.4:79801557-79801576	None:intergenic	40.0%
	TTCACAATAGGAGATAGCTG+TGG	+	chr3.4:79801630-79801649	None:intergenic	40.0%
!	AATGTTGCTTGCATTTGCTG+TGG	-	chr3.4:79801440-79801459	MS.gene066812:CDS	40.0%
	ATAGGAGATAGCTGTGGCTT+CGG	+	chr3.4:79801624-79801643	None:intergenic	45.0%
	CGGTTGAAAAAGGGCCAATT+TGG	+	chr3.4:79801558-79801577	None:intergenic	45.0%
	TGCTGTGGTTGCATTTGGAA+CGG	-	chr3.4:79801455-79801474	MS.gene066812:CDS	45.0%
!	AGAGGATGGCGGTTGAAAAA+GGG	+	chr3.4:79801567-79801586	None:intergenic	45.0%
!	ATTTGGGACAGTCACCAATG+TGG	+	chr3.4:79801541-79801560	None:intergenic	45.0%
!	CATTGGTGACTGTCCCAAAT+TGG	-	chr3.4:79801541-79801560	MS.gene066812:CDS	45.0%
!	GGATGCTTTTCTACCTCACT+TGG	+	chr3.4:79801603-79801622	None:intergenic	45.0%
!	TCTGCATTAGCTTCTCTGCT+AGG	+	chr3.4:79801419-79801438	None:intergenic	45.0%
!!	GCATTTGCTGTGGTTGCATT+TGG	-	chr3.4:79801450-79801469	MS.gene066812:CDS	45.0%
!!	TGGTGTTGTTCTCACCACAT+TGG	-	chr3.4:79801524-79801543	MS.gene066812:CDS	45.0%
	CAAGTGGTTGGATCCAAGTG+AGG	-	chr3.4:79801587-79801606	MS.gene066812:CDS	50.0%
	CTTGGATCCAACCACTTGAG+AGG	+	chr3.4:79801585-79801604	None:intergenic	50.0%
	GATCCAACCACTTGAGAGGA+TGG	+	chr3.4:79801581-79801600	None:intergenic	50.0%
!	GAGAGGATGGCGGTTGAAAA+AGG	+	chr3.4:79801568-79801587	None:intergenic	50.0%
!	GCTGTGGTTGCATTTGGAAC+GGG	-	chr3.4:79801456-79801475	MS.gene066812:CDS	50.0%
	CCAACCACTTGAGAGGATGG+CGG	+	chr3.4:79801578-79801597	None:intergenic	55.0%
	TCAACCGCCATCCTCTCAAG+TGG	-	chr3.4:79801571-79801590	MS.gene066812:CDS	55.0%
	CCGCCATCCTCTCAAGTGGT+TGG	-	chr3.4:79801575-79801594	MS.gene066812:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	79801396	79801673	79801396	ID=MS.gene066812
chr3.4	mRNA	79801396	79801673	79801396	ID=MS.gene066812.t1;Parent=MS.gene066812
chr3.4	exon	79801396	79801673	79801396	ID=MS.gene066812.t1.exon1;Parent=MS.gene066812.t1
chr3.4	CDS	79801396	79801673	79801396	ID=cds.MS.gene066812.t1;Parent=MS.gene066812.t1

Gene Sequence	>MS.gene066812 ATGGATTGGCAAGGGCAAAACCTAGCAGAGAAGCTAATGCAGATAATGTTGCTTGCATTTGCTGTGGTTGCATTTGGAACGGGATATCTTACAGCTTCTTTCAAGACGATGATGCTCATATATGCTGGTGGTGTTGTTCTCACCACATTGGTGACTGTCCCAAATTGGCCCTTTTTCAACCGCCATCCTCTCAAGTGGTTGGATCCAAGTGAGGTAGAAAAGCATCCGAAGCCACAGCTATCTCCTATTGTGAATTCAAAGAAGAAACCCATTAAGAA
Protein sequence	>MS.gene066812.t1 MDWQGQNLAEKLMQIMLLAFAVVAFGTGYLTASFKTMMLIYAGGVVLTTLVTVPNWPFFNRHPLKWLDPSEVEKHPKPQLSPIVNSKKKPIK