Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066829.t1 | XP_003601799.1 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 7.70E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066829.t1 | Q944J0 | 71.6 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 6.4e-31 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066829.t1 | G7JC07 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 5.6e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene066829 | MS.gene89036 | PPI |
| MS.gene066829 | MS.gene36215 | PPI |
| MS.gene066829 | MS.gene86381 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066829.t1 | MTR_3g085510 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 2.23e-64 | 189 |
| MS.gene066829.t1 | MTR_8g080380 | 78.495 | 93 | 20 | 0 | 1 | 93 | 92 | 184 | 3.90e-52 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066829.t1 | AT4G40042 | 79.747 | 79 | 16 | 0 | 1 | 79 | 1 | 79 | 1.26e-43 | 137 |
| MS.gene066829.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
| MS.gene066829.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
Find 26 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTACCCTACTTCTTAA+TGG | 0.179148 | 3.4:-79876931 | None:intergenic |
| GAGAGGATGGCGGTTGAAAA+AGG | 0.272451 | 3.4:-79876832 | None:intergenic |
| CATTGGTGACTGTCCCAAAT+TGG | 0.297025 | 3.4:+79876808 | MS.gene066829:CDS |
| TCTTTACCCTACTTCTTAAT+GGG | 0.300035 | 3.4:-79876930 | None:intergenic |
| GCATTTGCTGTGGTTGCATT+TGG | 0.308000 | 3.4:+79876717 | MS.gene066829:CDS |
| TTCTTCTTTGAATTCACAAT+AGG | 0.417935 | 3.4:-79876906 | None:intergenic |
| GAAGAAACCCATTAAGAAGT+AGG | 0.438658 | 3.4:+79876923 | MS.gene066829:CDS |
| TCTGCATTAGCTTCTCTGCT+AGG | 0.440951 | 3.4:-79876683 | None:intergenic |
| ATAGGAGATAGCTGTGGCTT+CGG | 0.450784 | 3.4:-79876888 | None:intergenic |
| GCTGTGGTTGCATTTGGAAC+GGG | 0.459654 | 3.4:+79876723 | MS.gene066829:CDS |
| TGCTGTGGTTGCATTTGGAA+CGG | 0.510809 | 3.4:+79876722 | MS.gene066829:CDS |
| AAGAAACCCATTAAGAAGTA+GGG | 0.531311 | 3.4:+79876924 | MS.gene066829:CDS |
| TTCATCAGATGGATTGGCAA+GGG | 0.536217 | 3.4:+79876655 | None:intergenic |
| CCGCCATCCTCTCAAGTGGT+TGG | 0.536748 | 3.4:+79876842 | MS.gene066829:CDS |
| TCAACCGCCATCCTCTCAAG+TGG | 0.553865 | 3.4:+79876838 | MS.gene066829:CDS |
| GATCCAACCACTTGAGAGGA+TGG | 0.566183 | 3.4:-79876845 | None:intergenic |
| CTTCATCAGATGGATTGGCA+AGG | 0.566563 | 3.4:+79876654 | None:intergenic |
| CTTGGATCCAACCACTTGAG+AGG | 0.567895 | 3.4:-79876849 | None:intergenic |
| AATGTTGCTTGCATTTGCTG+TGG | 0.601891 | 3.4:+79876707 | MS.gene066829:CDS |
| ACGATGATGCTCATATATGC+TGG | 0.624836 | 3.4:+79876768 | MS.gene066829:CDS |
| TGGTGTTGTTCTCACCACAT+TGG | 0.662379 | 3.4:+79876791 | MS.gene066829:CDS |
| CAAGTGGTTGGATCCAAGTG+AGG | 0.664517 | 3.4:+79876854 | MS.gene066829:CDS |
| ATGATGCTCATATATGCTGG+TGG | 0.699379 | 3.4:+79876771 | MS.gene066829:CDS |
| CCAACCACTTGAGAGGATGG+CGG | 0.730520 | 3.4:-79876842 | None:intergenic |
| ATTTGGGACAGTCACCAATG+TGG | 0.730637 | 3.4:-79876805 | None:intergenic |
| TTCACAATAGGAGATAGCTG+TGG | 0.732645 | 3.4:-79876894 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTCTTTGAATTCACAAT+AGG | - | chr3.4:79876909-79876928 | None:intergenic | 25.0% |
| ACGATGATGCTCATATATGC+TGG | + | chr3.4:79876768-79876787 | MS.gene066829:CDS | 40.0% | |
| ATGATGCTCATATATGCTGG+TGG | + | chr3.4:79876771-79876790 | MS.gene066829:CDS | 40.0% | |
| GGTTGAAAAAGGGCCAATTT+GGG | - | chr3.4:79876824-79876843 | None:intergenic | 40.0% | |
| TTCACAATAGGAGATAGCTG+TGG | - | chr3.4:79876897-79876916 | None:intergenic | 40.0% | |
| ! | AATGTTGCTTGCATTTGCTG+TGG | + | chr3.4:79876707-79876726 | MS.gene066829:CDS | 40.0% |
| ATAGGAGATAGCTGTGGCTT+CGG | - | chr3.4:79876891-79876910 | None:intergenic | 45.0% | |
| CGGTTGAAAAAGGGCCAATT+TGG | - | chr3.4:79876825-79876844 | None:intergenic | 45.0% | |
| TGCTGTGGTTGCATTTGGAA+CGG | + | chr3.4:79876722-79876741 | MS.gene066829:CDS | 45.0% | |
| ! | AGAGGATGGCGGTTGAAAAA+GGG | - | chr3.4:79876834-79876853 | None:intergenic | 45.0% |
| ! | ATTTGGGACAGTCACCAATG+TGG | - | chr3.4:79876808-79876827 | None:intergenic | 45.0% |
| ! | CATTGGTGACTGTCCCAAAT+TGG | + | chr3.4:79876808-79876827 | MS.gene066829:CDS | 45.0% |
| ! | GGATGCTTTTCTACCTCACT+TGG | - | chr3.4:79876870-79876889 | None:intergenic | 45.0% |
| ! | TCTGCATTAGCTTCTCTGCT+AGG | - | chr3.4:79876686-79876705 | None:intergenic | 45.0% |
| !! | GCATTTGCTGTGGTTGCATT+TGG | + | chr3.4:79876717-79876736 | MS.gene066829:CDS | 45.0% |
| !! | TGGTGTTGTTCTCACCACAT+TGG | + | chr3.4:79876791-79876810 | MS.gene066829:CDS | 45.0% |
| CAAGTGGTTGGATCCAAGTG+AGG | + | chr3.4:79876854-79876873 | MS.gene066829:CDS | 50.0% | |
| CTTGGATCCAACCACTTGAG+AGG | - | chr3.4:79876852-79876871 | None:intergenic | 50.0% | |
| GATCCAACCACTTGAGAGGA+TGG | - | chr3.4:79876848-79876867 | None:intergenic | 50.0% | |
| ! | GAGAGGATGGCGGTTGAAAA+AGG | - | chr3.4:79876835-79876854 | None:intergenic | 50.0% |
| ! | GCTGTGGTTGCATTTGGAAC+GGG | + | chr3.4:79876723-79876742 | MS.gene066829:CDS | 50.0% |
| CCAACCACTTGAGAGGATGG+CGG | - | chr3.4:79876845-79876864 | None:intergenic | 55.0% | |
| TCAACCGCCATCCTCTCAAG+TGG | + | chr3.4:79876838-79876857 | MS.gene066829:CDS | 55.0% | |
| CCGCCATCCTCTCAAGTGGT+TGG | + | chr3.4:79876842-79876861 | MS.gene066829:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 79876663 | 79876944 | 79876663 | ID=MS.gene066829 |
| chr3.4 | mRNA | 79876663 | 79876944 | 79876663 | ID=MS.gene066829.t1;Parent=MS.gene066829 |
| chr3.4 | exon | 79876663 | 79876944 | 79876663 | ID=MS.gene066829.t1.exon1;Parent=MS.gene066829.t1 |
| chr3.4 | CDS | 79876663 | 79876944 | 79876663 | ID=cds.MS.gene066829.t1;Parent=MS.gene066829.t1 |
| Gene Sequence |
| Protein sequence |
