Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066963.t1 | RHN59870.1 | 51.5 | 134 | 53 | 3 | 16 | 138 | 296 | 428 | 1.30E-24 | 122.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066963.t1 | A0A396I2Q7 | 51.5 | 134 | 53 | 3 | 16 | 138 | 296 | 428 | 9.6e-25 | 122.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050762 | MS.gene066963 | 0.806633 | 7.48E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene066963 | 0.807477 | 4.96E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene066963 | 0.816962 | 4.22E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene066963 | 0.819811 | 9.55E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066963.t1 | MTR_3g436320 | 60.550 | 109 | 33 | 3 | 7 | 111 | 45 | 147 | 2.16e-33 | 115 |
| MS.gene066963.t1 | MTR_6g022470 | 49.640 | 139 | 56 | 5 | 17 | 141 | 56 | 194 | 3.24e-33 | 118 |
| MS.gene066963.t1 | MTR_2g461790 | 44.304 | 79 | 44 | 0 | 14 | 92 | 20 | 98 | 1.50e-15 | 70.5 |
| MS.gene066963.t1 | MTR_6g462630 | 37.143 | 105 | 66 | 0 | 18 | 122 | 78 | 182 | 4.33e-15 | 69.7 |
| MS.gene066963.t1 | MTR_5g081480 | 42.105 | 76 | 43 | 1 | 18 | 92 | 700 | 775 | 5.20e-14 | 68.6 |
| MS.gene066963.t1 | MTR_2g048835 | 44.737 | 76 | 41 | 1 | 17 | 91 | 349 | 424 | 1.45e-13 | 67.4 |
| MS.gene066963.t1 | MTR_2g090645 | 38.384 | 99 | 52 | 3 | 23 | 120 | 2 | 92 | 9.91e-13 | 63.9 |
| MS.gene066963.t1 | MTR_3g049490 | 49.231 | 65 | 29 | 2 | 31 | 91 | 5 | 69 | 7.35e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATCAGGATAGTTAGTTT+TGG | 0.296316 | 6.4:-47902988 | None:intergenic |
| TCTTTGGTTGATTAACAATC+TGG | 0.322395 | 6.4:-47903280 | None:intergenic |
| TTTGTAGATGTGGTTTCATC+TGG | 0.346888 | 6.4:-47903068 | None:intergenic |
| TAAGCAGGTGGCAGAATATC+AGG | 0.353783 | 6.4:-47903002 | None:intergenic |
| CTTATGTTCATAGGTACTAT+AGG | 0.355669 | 6.4:+47902903 | MS.gene066963:CDS |
| ATGAAACCACATCTACAAAT+AGG | 0.365104 | 6.4:+47903072 | MS.gene066963:CDS |
| CTAGGACAAGAACTACAAAT+AGG | 0.413048 | 6.4:+47903099 | MS.gene066963:CDS |
| CTATTCTGATTGTGAGGTGC+AGG | 0.442103 | 6.4:-47903221 | None:intergenic |
| TGTTAATCAACCAAAGAAAC+AGG | 0.446000 | 6.4:+47903286 | MS.gene066963:CDS |
| ACCCACAAACTTATGTTCAT+AGG | 0.456194 | 6.4:+47902894 | MS.gene066963:CDS |
| TGGAATATGGTCACAATGCT+AGG | 0.457169 | 6.4:+47903135 | MS.gene066963:CDS |
| TGTAATGTGTGCTTGGAATA+TGG | 0.458970 | 6.4:+47903122 | MS.gene066963:CDS |
| AGTCCACCTATTTGTAGATG+TGG | 0.463001 | 6.4:-47903078 | None:intergenic |
| CCTGCTTACAAGCGTGGTCC+TGG | 0.467454 | 6.4:+47903017 | MS.gene066963:CDS |
| CACATGTCCTGCCCATTGAT+AGG | 0.475639 | 6.4:-47902963 | None:intergenic |
| GAAGGCATTACACCTATCAA+TGG | 0.491396 | 6.4:+47902951 | MS.gene066963:CDS |
| GCACCTCACAATCAGAATAG+TGG | 0.501810 | 6.4:+47903224 | MS.gene066963:CDS |
| TACCTATGAACATAAGTTTG+TGG | 0.505178 | 6.4:-47902896 | None:intergenic |
| ACCTATGAACATAAGTTTGT+GGG | 0.512160 | 6.4:-47902895 | None:intergenic |
| GAAGAAAGTTCCTGGTAGGA+TGG | 0.513425 | 6.4:+47903689 | MS.gene066963:CDS |
| CATCTACAAATAGGTGGACT+AGG | 0.514726 | 6.4:+47903081 | MS.gene066963:CDS |
| AAGAAAGTTCCTGGTAGGAT+GGG | 0.525370 | 6.4:+47903690 | MS.gene066963:CDS |
| AAATAGGTGTAATGTGTGCT+TGG | 0.539054 | 6.4:+47903115 | MS.gene066963:CDS |
| CCAGGACCACGCTTGTAAGC+AGG | 0.541206 | 6.4:-47903017 | None:intergenic |
| CAATGCTAGGACATGTAAGA+AGG | 0.543980 | 6.4:+47903148 | MS.gene066963:CDS |
| TAAAGAAGAAAGTTCCTGGT+AGG | 0.547844 | 6.4:+47903685 | MS.gene066963:CDS |
| AAGGCATTACACCTATCAAT+GGG | 0.552118 | 6.4:+47902952 | MS.gene066963:CDS |
| TAATTGATCCCCATCCTACC+AGG | 0.559440 | 6.4:-47903699 | None:intergenic |
| CTTACAAGCGTGGTCCTGGT+AGG | 0.572560 | 6.4:+47903021 | MS.gene066963:CDS |
| AGAAAGTTCCTGGTAGGATG+GGG | 0.573754 | 6.4:+47903691 | MS.gene066963:CDS |
| TGGACAAGCTGCTGCAGTGT+TGG | 0.577032 | 6.4:+47903244 | MS.gene066963:CDS |
| GCTGCAGTGTTGGCAACACA+AGG | 0.577898 | 6.4:+47903254 | MS.gene066963:CDS |
| CATTACACCTATCAATGGGC+AGG | 0.578236 | 6.4:+47902956 | MS.gene066963:CDS |
| AATATAGAGTGTTACACTGA+AGG | 0.588127 | 6.4:+47902933 | MS.gene066963:CDS |
| CTATCAATGGGCAGGACATG+TGG | 0.597509 | 6.4:+47902964 | MS.gene066963:CDS |
| CTGCCACCTGCTTACAAGCG+TGG | 0.598269 | 6.4:+47903011 | MS.gene066963:CDS |
| TGTCCACTATTCTGATTGTG+AGG | 0.612949 | 6.4:-47903227 | None:intergenic |
| GGTGCAGGCTCAGTTTGAAG+TGG | 0.620952 | 6.4:-47903206 | None:intergenic |
| AAACCACATCTACAAATAGG+TGG | 0.629469 | 6.4:+47903075 | MS.gene066963:CDS |
| GGACCACGCTTGTAAGCAGG+TGG | 0.642148 | 6.4:-47903014 | None:intergenic |
| CTGCAGTGTTGGCAACACAA+GGG | 0.667451 | 6.4:+47903255 | MS.gene066963:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAGAAAAAAAATTAAGAAA+GGG | + | chr6.4:47903442-47903461 | MS.gene066963:intron | 15.0% |
| !! | TTTATCATGTAAAATTCATT+TGG | - | chr6.4:47903351-47903370 | None:intergenic | 15.0% |
| !! | AATCAAATACGTACATAATT+TGG | - | chr6.4:47903582-47903601 | None:intergenic | 20.0% |
| !! | ACTTGTTTGTATGAATAATT+AGG | + | chr6.4:47903370-47903389 | MS.gene066963:intron | 20.0% |
| !! | CTTGTTTGTATGAATAATTA+GGG | + | chr6.4:47903371-47903390 | MS.gene066963:intron | 20.0% |
| !! | GTCAGAAAAAAAATTAAGAA+AGG | + | chr6.4:47903441-47903460 | MS.gene066963:intron | 20.0% |
| !! | GTGAAATACATTTATTTGAA+GGG | - | chr6.4:47903325-47903344 | None:intergenic | 20.0% |
| !! | TGTGAAATACATTTATTTGA+AGG | - | chr6.4:47903326-47903345 | None:intergenic | 20.0% |
| !! | TTGTTTGTATGAATAATTAG+GGG | + | chr6.4:47903372-47903391 | MS.gene066963:intron | 20.0% |
| !!! | AAAATTTTTACCTGTTTCTT+TGG | - | chr6.4:47903299-47903318 | None:intergenic | 20.0% |
| !!! | TCTTAATTTTTTTTCTGACT+TGG | - | chr6.4:47903440-47903459 | None:intergenic | 20.0% |
| !! | TTTATTTCTTTGCAGTACAT+AGG | + | chr6.4:47903600-47903619 | MS.gene066963:intron | 25.0% |
| !!! | AATATCAGGATAGTTAGTTT+TGG | - | chr6.4:47902991-47903010 | None:intergenic | 25.0% |
| !!! | ATATCAGGATAGTTAGTTTT+GGG | - | chr6.4:47902990-47903009 | None:intergenic | 25.0% |
| AAAATTAAGAAAGGGACACA+AGG | + | chr6.4:47903450-47903469 | MS.gene066963:intron | 30.0% | |
| AATAATTAGGGGAGATCTAA+GGG | + | chr6.4:47903383-47903402 | MS.gene066963:intron | 30.0% | |
| ACCTATGAACATAAGTTTGT+GGG | - | chr6.4:47902898-47902917 | None:intergenic | 30.0% | |
| ATGAAACCACATCTACAAAT+AGG | + | chr6.4:47903072-47903091 | MS.gene066963:CDS | 30.0% | |
| CTTATGTTCATAGGTACTAT+AGG | + | chr6.4:47902903-47902922 | MS.gene066963:CDS | 30.0% | |
| GGAAAAAAAAGAAGTTACTG+TGG | + | chr6.4:47903169-47903188 | MS.gene066963:CDS | 30.0% | |
| TACCTATGAACATAAGTTTG+TGG | - | chr6.4:47902899-47902918 | None:intergenic | 30.0% | |
| TCTTTGGTTGATTAACAATC+TGG | - | chr6.4:47903283-47903302 | None:intergenic | 30.0% | |
| TGTTAATCAACCAAAGAAAC+AGG | + | chr6.4:47903286-47903305 | MS.gene066963:CDS | 30.0% | |
| ! | AATATAGAGTGTTACACTGA+AGG | + | chr6.4:47902933-47902952 | MS.gene066963:CDS | 30.0% |
| !!! | GTTTTAAAGAAGAAAGTTCC+TGG | + | chr6.4:47903681-47903700 | MS.gene066963:CDS | 30.0% |
| AAACCACATCTACAAATAGG+TGG | + | chr6.4:47903075-47903094 | MS.gene066963:CDS | 35.0% | |
| AAATAGGTGTAATGTGTGCT+TGG | + | chr6.4:47903115-47903134 | MS.gene066963:CDS | 35.0% | |
| ACCCACAAACTTATGTTCAT+AGG | + | chr6.4:47902894-47902913 | MS.gene066963:CDS | 35.0% | |
| CTAGGACAAGAACTACAAAT+AGG | + | chr6.4:47903099-47903118 | MS.gene066963:CDS | 35.0% | |
| GAATAATTAGGGGAGATCTA+AGG | + | chr6.4:47903382-47903401 | MS.gene066963:intron | 35.0% | |
| TAAAGAAGAAAGTTCCTGGT+AGG | + | chr6.4:47903685-47903704 | MS.gene066963:CDS | 35.0% | |
| TGTAATGTGTGCTTGGAATA+TGG | + | chr6.4:47903122-47903141 | MS.gene066963:CDS | 35.0% | |
| TTTGTAGATGTGGTTTCATC+TGG | - | chr6.4:47903071-47903090 | None:intergenic | 35.0% | |
| ! | AAGGCATTACACCTATCAAT+GGG | + | chr6.4:47902952-47902971 | MS.gene066963:CDS | 35.0% |
| !!! | TCTCTCCTCCTCAATTTTTT+TGG | - | chr6.4:47903047-47903066 | None:intergenic | 35.0% |
| !!! | TGTTTTGACTGTGTTTTGCA+GGG | + | chr6.4:47903654-47903673 | MS.gene066963:intron | 35.0% |
| !!! | TTGTTTTGACTGTGTTTTGC+AGG | + | chr6.4:47903653-47903672 | MS.gene066963:intron | 35.0% |
| AAGAAAGTTCCTGGTAGGAT+GGG | + | chr6.4:47903690-47903709 | MS.gene066963:CDS | 40.0% | |
| AGTCCACCTATTTGTAGATG+TGG | - | chr6.4:47903081-47903100 | None:intergenic | 40.0% | |
| CAATGCTAGGACATGTAAGA+AGG | + | chr6.4:47903148-47903167 | MS.gene066963:CDS | 40.0% | |
| CTGGTAGGCCAAAAAAATTG+AGG | + | chr6.4:47903036-47903055 | MS.gene066963:CDS | 40.0% | |
| GTAGGCCAAAAAAATTGAGG+AGG | + | chr6.4:47903039-47903058 | MS.gene066963:CDS | 40.0% | |
| TGGAATATGGTCACAATGCT+AGG | + | chr6.4:47903135-47903154 | MS.gene066963:CDS | 40.0% | |
| TGTCCACTATTCTGATTGTG+AGG | - | chr6.4:47903230-47903249 | None:intergenic | 40.0% | |
| ! | CATCTACAAATAGGTGGACT+AGG | + | chr6.4:47903081-47903100 | MS.gene066963:CDS | 40.0% |
| !! | GAAGGCATTACACCTATCAA+TGG | + | chr6.4:47902951-47902970 | MS.gene066963:CDS | 40.0% |
| !!! | CTCAATTTTTTTGGCCTACC+AGG | - | chr6.4:47903038-47903057 | None:intergenic | 40.0% |
| !!! | TTTTTCTGACTTGGTCCCTT+TGG | - | chr6.4:47903431-47903450 | None:intergenic | 40.0% |
| AAGAAAGGGACACAAGGTGA+TGG | + | chr6.4:47903456-47903475 | MS.gene066963:intron | 45.0% | |
| AGAAAGTTCCTGGTAGGATG+GGG | + | chr6.4:47903691-47903710 | MS.gene066963:CDS | 45.0% | |
| ATCTAAGGGTCCTGCTATGA+AGG | + | chr6.4:47903397-47903416 | MS.gene066963:intron | 45.0% | |
| CATTACACCTATCAATGGGC+AGG | + | chr6.4:47902956-47902975 | MS.gene066963:CDS | 45.0% | |
| CTATTCTGATTGTGAGGTGC+AGG | - | chr6.4:47903224-47903243 | None:intergenic | 45.0% | |
| GAAGAAAGTTCCTGGTAGGA+TGG | + | chr6.4:47903689-47903708 | MS.gene066963:CDS | 45.0% | |
| GCAACAGTAGGTTCTTGAAG+TGG | - | chr6.4:47903528-47903547 | None:intergenic | 45.0% | |
| GCACCTCACAATCAGAATAG+TGG | + | chr6.4:47903224-47903243 | MS.gene066963:CDS | 45.0% | |
| TAAGCAGGTGGCAGAATATC+AGG | - | chr6.4:47903005-47903024 | None:intergenic | 45.0% | |
| TAATTGATCCCCATCCTACC+AGG | - | chr6.4:47903702-47903721 | None:intergenic | 45.0% | |
| ATGAAGGCCGGCAAACCAAA+GGG | + | chr6.4:47903413-47903432 | MS.gene066963:intron | 50.0% | |
| CACATGTCCTGCCCATTGAT+AGG | - | chr6.4:47902966-47902985 | None:intergenic | 50.0% | |
| CTATCAATGGGCAGGACATG+TGG | + | chr6.4:47902964-47902983 | MS.gene066963:CDS | 50.0% | |
| GCAGCAACTTCAGCAGGTTT+CGG | - | chr6.4:47903498-47903517 | None:intergenic | 50.0% | |
| GTTTCAGCAGCAACTTCAGC+AGG | - | chr6.4:47903504-47903523 | None:intergenic | 50.0% | |
| TATGAAGGCCGGCAAACCAA+AGG | + | chr6.4:47903412-47903431 | MS.gene066963:intron | 50.0% | |
| TGACTACGTGCTGCAACAGT+AGG | - | chr6.4:47903540-47903559 | None:intergenic | 50.0% | |
| !! | ACTTGGTCCCTTTGGTTTGC+CGG | - | chr6.4:47903423-47903442 | None:intergenic | 50.0% |
| !! | CTGCAGTGTTGGCAACACAA+GGG | + | chr6.4:47903255-47903274 | MS.gene066963:CDS | 50.0% |
| AAGGGTCCTGCTATGAAGGC+CGG | + | chr6.4:47903401-47903420 | MS.gene066963:intron | 55.0% | |
| CTTACAAGCGTGGTCCTGGT+AGG | + | chr6.4:47903021-47903040 | MS.gene066963:CDS | 55.0% | |
| GGTGCAGGCTCAGTTTGAAG+TGG | - | chr6.4:47903209-47903228 | None:intergenic | 55.0% | |
| TGGACAAGCTGCTGCAGTGT+TGG | + | chr6.4:47903244-47903263 | MS.gene066963:CDS | 55.0% | |
| !! | GCTGCAGTGTTGGCAACACA+AGG | + | chr6.4:47903254-47903273 | MS.gene066963:CDS | 55.0% |
| CCAGGACCACGCTTGTAAGC+AGG | - | chr6.4:47903020-47903039 | None:intergenic | 60.0% | |
| CCTGCTTACAAGCGTGGTCC+TGG | + | chr6.4:47903017-47903036 | MS.gene066963:CDS | 60.0% | |
| CTGCCACCTGCTTACAAGCG+TGG | + | chr6.4:47903011-47903030 | MS.gene066963:CDS | 60.0% | |
| GGACCACGCTTGTAAGCAGG+TGG | - | chr6.4:47903017-47903036 | None:intergenic | 60.0% | |
| GGTTTGCCGGCCTTCATAGC+AGG | - | chr6.4:47903410-47903429 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 47902891 | 47903722 | 47902891 | ID=MS.gene066963 |
| chr6.4 | mRNA | 47902891 | 47903722 | 47902891 | ID=MS.gene066963.t1;Parent=MS.gene066963 |
| chr6.4 | exon | 47902891 | 47903307 | 47902891 | ID=MS.gene066963.t1.exon1;Parent=MS.gene066963.t1 |
| chr6.4 | CDS | 47902891 | 47903307 | 47902891 | ID=cds.MS.gene066963.t1;Parent=MS.gene066963.t1 |
| chr6.4 | exon | 47903675 | 47903722 | 47903675 | ID=MS.gene066963.t1.exon2;Parent=MS.gene066963.t1 |
| chr6.4 | CDS | 47903675 | 47903722 | 47903675 | ID=cds.MS.gene066963.t1;Parent=MS.gene066963.t1 |
| Gene Sequence |
| Protein sequence |
