AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067826

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067826.t1 MTR_7g086000 94.194 465 26 1 1 464 1 465 0.0 871
MS.gene067826.t1 MTR_7g085990 44.654 477 236 12 4 464 2 466 4.01e-132 390
MS.gene067826.t1 MTR_7g085930 44.351 478 236 12 4 464 6 470 9.39e-126 374
MS.gene067826.t1 MTR_7g085960 72.368 228 33 2 237 464 1 198 4.13e-114 334
MS.gene067826.t1 MTR_4g127650 38.745 462 259 13 4 456 10 456 3.65e-95 295
MS.gene067826.t1 MTR_3g049150 36.325 468 278 12 4 464 2 456 2.73e-93 290
MS.gene067826.t1 MTR_3g026950 37.259 467 262 14 4 456 2 451 3.64e-87 274
MS.gene067826.t1 MTR_4g013050 36.226 461 238 11 1 456 1 410 2.06e-86 271
MS.gene067826.t1 MTR_4g097090 37.445 454 265 12 10 456 15 456 8.13e-86 271
MS.gene067826.t1 MTR_4g007540 33.700 454 274 13 4 454 3 432 2.26e-80 256
MS.gene067826.t1 MTR_2g105330 33.040 454 276 13 4 454 3 431 1.43e-79 254
MS.gene067826.t1 MTR_2g064495 32.599 454 278 13 4 454 3 431 9.95e-78 249
MS.gene067826.t1 MTR_8g075610 34.346 428 249 14 31 454 20 419 7.35e-76 244
MS.gene067826.t1 MTR_3g098980 33.415 410 226 18 12 412 22 393 3.10e-54 187
MS.gene067826.t1 MTR_8g096530 32.057 418 242 14 2 412 5 387 3.87e-53 185
MS.gene067826.t1 MTR_7g027175 32.178 404 232 15 11 407 23 391 4.68e-50 176
MS.gene067826.t1 MTR_7g085970 60.811 148 28 4 43 189 8 126 4.16e-47 159
MS.gene067826.t1 MTR_1g040215 27.407 405 250 14 12 407 17 386 8.50e-40 149
MS.gene067826.t1 MTR_1g040175 28.747 407 242 17 11 406 16 385 1.57e-39 148
MS.gene067826.t1 MTR_3g049140 35.496 262 152 11 210 464 2 253 1.85e-39 143
MS.gene067826.t1 MTR_7g084940 27.765 425 268 15 2 420 14 405 9.81e-39 146
MS.gene067826.t1 MTR_7g084910 28.868 433 239 18 2 420 15 392 4.37e-38 144
MS.gene067826.t1 MTR_1g053315 28.274 481 292 20 2 460 16 465 3.95e-36 139
MS.gene067826.t1 MTR_5g076700 25.951 447 277 17 11 440 17 426 5.36e-33 130
MS.gene067826.t1 MTR_5g090810 28.346 381 216 15 54 407 61 411 6.48e-33 130
MS.gene067826.t1 MTR_1g040195 27.020 396 235 18 73 454 55 410 8.92e-33 129
MS.gene067826.t1 MTR_7g114490 26.840 462 272 19 11 451 17 433 2.46e-32 129
MS.gene067826.t1 MTR_5g090830 28.609 381 215 15 54 407 61 411 3.85e-32 128
MS.gene067826.t1 MTR_3g101930 29.177 401 232 17 44 424 54 422 3.99e-32 127
MS.gene067826.t1 MTR_7g056087 24.781 456 292 15 1 440 1 421 5.62e-32 127
MS.gene067826.t1 MTR_7g114470 25.942 451 273 19 11 440 18 428 1.33e-31 126
MS.gene067826.t1 MTR_3g071320 28.108 370 244 11 3 362 4 361 3.91e-31 124
MS.gene067826.t1 MTR_1g081660 24.570 407 273 11 5 406 17 394 4.14e-31 125
MS.gene067826.t1 MTR_3g086020 25.800 469 279 18 2 460 3 412 1.08e-30 123
MS.gene067826.t1 MTR_2g015430 29.117 419 252 15 57 463 56 441 4.22e-30 122
MS.gene067826.t1 MTR_5g090880 27.020 396 231 16 42 407 46 413 5.08e-30 122
MS.gene067826.t1 MTR_1g040315 27.253 466 244 22 12 464 17 400 7.56e-30 120
MS.gene067826.t1 MTR_7g114550 25.466 483 288 22 1 459 7 441 1.16e-29 120
MS.gene067826.t1 MTR_7g114520 25.161 465 289 20 11 457 18 441 1.37e-29 120
MS.gene067826.t1 MTR_3g112180 28.700 446 254 18 12 438 20 420 3.36e-29 119
MS.gene067826.t1 MTR_3g465990 26.620 432 266 18 4 416 10 409 3.93e-29 119
MS.gene067826.t1 MTR_1g040320 25.495 404 223 12 11 406 16 349 4.16e-29 119
MS.gene067826.t1 MTR_3g101900 28.645 391 220 17 62 431 69 421 4.77e-29 119
MS.gene067826.t1 MTR_4g055170 25.926 432 264 16 2 412 10 406 7.89e-29 118
MS.gene067826.t1 MTR_3g465980 27.356 435 259 18 4 416 10 409 2.72e-28 117
MS.gene067826.t1 MTR_8g079980 26.712 438 272 18 6 433 6 404 2.89e-28 116
MS.gene067826.t1 MTR_7g114560 24.946 465 293 18 11 459 17 441 3.95e-28 116
MS.gene067826.t1 MTR_7g014200 23.975 488 310 17 3 456 8 468 5.40e-28 116
MS.gene067826.t1 MTR_7g014160 26.210 496 294 23 3 456 8 473 1.15e-27 115
MS.gene067826.t1 MTR_6g053810 28.312 385 232 17 3 365 8 370 2.61e-27 114
MS.gene067826.t1 MTR_3g070170 27.352 457 248 20 12 424 20 436 5.08e-27 113
MS.gene067826.t1 MTR_7g014250 24.792 480 307 14 5 456 3 456 7.19e-27 112
MS.gene067826.t1 MTR_2g007880 23.044 473 310 14 1 459 14 446 3.18e-26 111
MS.gene067826.t1 MTR_7g014330 26.216 473 294 15 9 456 14 456 3.43e-26 110
MS.gene067826.t1 MTR_3g465930 28.609 381 235 15 2 363 8 370 4.89e-26 110
MS.gene067826.t1 MTR_3g450710 26.064 376 219 15 59 406 65 409 1.14e-25 109
MS.gene067826.t1 MTR_3g101960 28.148 405 239 20 62 449 71 440 1.48e-25 108
MS.gene067826.t1 MTR_1g094790 25.118 422 268 12 2 407 1 390 2.15e-25 108
MS.gene067826.t1 MTR_3g102020 27.020 396 237 19 62 440 72 432 4.78e-25 107
MS.gene067826.t1 MTR_3g450690 26.005 373 220 15 59 406 72 413 7.28e-25 107
MS.gene067826.t1 MTR_0190s0020 27.628 409 239 17 59 449 63 432 7.58e-25 107
MS.gene067826.t1 MTR_5g026320 26.651 439 284 17 19 449 35 443 1.33e-24 106
MS.gene067826.t1 MTR_7g110610 26.316 456 239 21 11 453 12 383 2.08e-24 104
MS.gene067826.t1 MTR_7g014610 25.820 488 309 20 3 456 7 475 3.23e-24 105
MS.gene067826.t1 MTR_3g101970 27.009 448 254 20 19 440 18 418 6.92e-24 103
MS.gene067826.t1 MTR_3g465910 28.721 383 229 20 2 363 8 367 8.21e-24 104
MS.gene067826.t1 MTR_3g102010 27.000 400 236 19 62 440 68 432 9.52e-24 103
MS.gene067826.t1 MTR_7g014230 25.358 489 307 15 1 459 1 461 1.23e-23 103
MS.gene067826.t1 MTR_8g041770 26.859 417 254 16 47 448 50 430 1.26e-23 103
MS.gene067826.t1 MTR_6g045553 24.946 465 264 17 2 420 12 437 3.07e-23 102
MS.gene067826.t1 MTR_4g094842 26.464 461 279 19 9 448 16 437 6.84e-23 101
MS.gene067826.t1 MTR_5g084870 22.414 464 301 15 2 452 1 418 6.84e-23 101
MS.gene067826.t1 MTR_3g465950 28.440 327 204 11 2 313 8 319 1.36e-22 100
MS.gene067826.t1 MTR_8g079970 24.009 454 292 17 2 442 1 414 2.92e-22 99.4
MS.gene067826.t1 MTR_2g045550 37.241 145 80 2 1 145 1 134 5.23e-22 92.4
MS.gene067826.t1 MTR_3g465880 26.591 440 267 18 2 416 8 416 6.54e-22 98.6
MS.gene067826.t1 MTR_8g467580 25.236 424 258 15 4 407 1 385 1.20e-21 97.4
MS.gene067826.t1 MTR_7g014620 26.419 458 287 19 17 456 7 432 2.15e-21 96.7
MS.gene067826.t1 MTR_8g467590 24.078 461 284 18 11 449 10 426 2.72e-21 96.3
MS.gene067826.t1 MTR_6g015830 24.262 474 306 18 10 459 19 463 7.32e-21 95.1
MS.gene067826.t1 MTR_7g013850 23.146 445 263 17 59 456 68 480 1.36e-20 94.7
MS.gene067826.t1 MTR_0502s0010 29.771 262 144 12 98 344 2 238 1.83e-20 90.5
MS.gene067826.t1 MTR_5g081960 23.409 487 265 16 12 431 14 459 1.85e-20 94.4
MS.gene067826.t1 MTR_3g088420 27.072 362 225 16 62 413 58 390 2.83e-20 93.2
MS.gene067826.t1 MTR_3g465870 27.907 301 195 9 2 292 10 298 7.28e-20 92.0
MS.gene067826.t1 MTR_2g089735 25.858 437 272 16 1 407 1 415 6.47e-19 89.4
MS.gene067826.t1 MTR_7g026860 27.049 366 213 16 62 407 58 389 6.72e-19 89.4
MS.gene067826.t1 MTR_4g022825 26.933 401 240 19 59 440 55 421 7.21e-19 89.0
MS.gene067826.t1 MTR_4g022290 24.413 426 252 16 59 458 60 441 7.61e-19 89.0
MS.gene067826.t1 MTR_8g079710 26.161 409 234 15 59 440 59 426 7.75e-19 89.0
MS.gene067826.t1 MTR_4g094835 25.971 412 247 20 57 448 62 435 1.03e-18 88.6
MS.gene067826.t1 MTR_4g015180 26.850 473 267 23 12 431 14 460 1.90e-18 88.2
MS.gene067826.t1 MTR_5g084850 23.355 471 307 16 2 464 1 425 1.99e-18 87.8
MS.gene067826.t1 MTR_6g015315 20.542 443 298 13 39 457 41 453 7.08e-18 86.3
MS.gene067826.t1 MTR_3g026310 25.000 416 253 17 59 448 55 437 1.19e-17 85.5
MS.gene067826.t1 MTR_1g035890 26.518 313 193 11 9 308 14 302 1.38e-17 85.1
MS.gene067826.t1 MTR_2g089650 25.342 438 266 20 3 407 8 417 1.89e-17 85.1
MS.gene067826.t1 MTR_3g028210 26.790 433 259 19 6 407 11 416 2.03e-17 84.7
MS.gene067826.t1 MTR_4g022670 25.123 406 244 19 40 416 32 406 6.30e-17 83.2
MS.gene067826.t1 MTR_4g022720 26.185 401 248 19 62 448 58 424 1.26e-16 82.4
MS.gene067826.t1 MTR_2g089765 23.944 497 306 17 7 461 21 487 4.91e-16 80.9
MS.gene067826.t1 MTR_4g007460 34.722 144 78 5 74 215 2 131 1.02e-15 74.7
MS.gene067826.t1 MTR_4g022760 27.393 303 196 13 62 352 58 348 1.59e-15 78.6
MS.gene067826.t1 MTR_4g022800 25.266 376 223 17 62 413 58 399 2.98e-15 78.2
MS.gene067826.t1 MTR_3g087640 22.449 490 318 19 6 459 12 475 3.31e-15 78.2
MS.gene067826.t1 MTR_4g094838 24.590 427 236 17 49 448 54 421 8.80e-15 76.6
MS.gene067826.t1 MTR_3g100150 25.552 317 198 10 5 308 12 303 1.38e-14 76.3
MS.gene067826.t1 MTR_2g045540 39.048 105 50 4 210 313 2 93 1.06e-13 67.8
MS.gene067826.t1 MTR_2g036780 25.758 264 167 7 24 280 34 275 1.55e-13 72.8
MS.gene067826.t1 MTR_0042s0190 25.333 300 181 15 162 450 3 270 1.62e-13 71.6
MS.gene067826.t1 MTR_2g089670 24.615 390 248 14 40 407 64 429 2.07e-13 72.4
MS.gene067826.t1 MTR_7g014360 23.399 406 252 11 69 456 22 386 2.16e-13 72.0
MS.gene067826.t1 MTR_7g014630 28.796 191 108 7 3 176 8 187 8.68e-13 70.1
MS.gene067826.t1 MTR_7g114960 23.547 327 192 11 59 363 63 353 4.28e-12 68.2
MS.gene067826.t1 MTR_0042s0190 27.551 196 117 8 262 450 28 205 3.00e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067826.t1 AT2G19070 37.149 463 272 12 1 456 1 451 5.61e-100 307
MS.gene067826.t1 AT5G48930 32.237 456 282 12 2 454 1 432 3.00e-74 240
MS.gene067826.t1 AT5G57840 31.739 460 283 13 2 454 1 436 1.66e-64 215
MS.gene067826.t1 AT5G41040 31.250 416 242 16 2 407 13 394 8.74e-56 192
MS.gene067826.t1 AT5G41040 31.250 416 242 16 2 407 29 410 9.54e-56 192
MS.gene067826.t1 AT3G48720 32.518 409 224 20 11 407 16 384 2.45e-48 172
MS.gene067826.t1 AT5G63560 31.450 407 227 16 11 407 16 380 1.89e-46 166
MS.gene067826.t1 AT1G65450 26.866 469 294 15 2 456 16 449 1.55e-40 151
MS.gene067826.t1 AT5G17540 25.261 479 299 22 3 464 8 444 1.16e-35 138
MS.gene067826.t1 AT3G03480 25.833 480 297 18 2 463 15 453 1.26e-35 137
MS.gene067826.t1 AT3G26040 27.609 460 288 16 11 464 10 430 1.43e-35 137
MS.gene067826.t1 AT1G24430 28.211 436 270 14 2 433 3 399 2.13e-34 134
MS.gene067826.t1 AT2G40230 28.777 417 249 14 4 407 5 386 4.91e-34 132
MS.gene067826.t1 AT1G27620 28.667 450 272 18 11 453 16 423 1.95e-33 131
MS.gene067826.t1 AT3G29635 25.322 466 286 16 22 459 26 457 2.61e-33 131
MS.gene067826.t1 AT1G03390 27.119 472 292 18 2 452 15 455 4.86e-33 130
MS.gene067826.t1 AT1G78990 27.978 361 216 14 59 407 67 395 2.34e-32 128
MS.gene067826.t1 AT1G32910 27.123 365 233 13 52 407 58 398 4.08e-32 128
MS.gene067826.t1 AT5G07080 28.571 413 258 18 11 412 21 407 4.40e-32 127
MS.gene067826.t1 AT3G30280 26.258 457 278 14 11 450 13 427 1.78e-30 123
MS.gene067826.t1 AT3G62160 25.476 420 241 17 11 412 12 377 6.39e-30 121
MS.gene067826.t1 AT4G15390 26.782 463 269 18 11 450 15 430 2.36e-29 120
MS.gene067826.t1 AT4G15390 26.782 463 269 18 11 450 15 430 2.36e-29 120
MS.gene067826.t1 AT1G28680 25.995 427 265 14 1 412 1 391 3.50e-29 119
MS.gene067826.t1 AT2G25150 27.714 433 274 16 11 432 20 424 1.70e-28 117
MS.gene067826.t1 AT3G29670 25.000 424 272 17 44 452 53 445 1.71e-28 117
MS.gene067826.t1 AT3G47170 27.835 388 238 16 11 372 19 390 8.95e-26 110
MS.gene067826.t1 AT5G16410 24.384 365 227 14 57 407 89 418 1.24e-25 109
MS.gene067826.t1 AT2G23510 26.699 412 263 16 11 412 20 402 2.96e-25 108
MS.gene067826.t1 AT5G47950 26.077 441 262 17 11 440 13 400 4.26e-25 107
MS.gene067826.t1 AT5G23940 27.027 407 254 16 59 450 65 443 8.21e-25 107
MS.gene067826.t1 AT3G29590 26.619 417 246 16 66 457 68 449 8.54e-25 107
MS.gene067826.t1 AT1G24420 24.580 476 296 15 3 464 4 430 1.39e-24 106
MS.gene067826.t1 AT1G24420 24.580 476 296 15 3 464 4 430 1.39e-24 106
MS.gene067826.t1 AT1G31490 24.939 413 272 13 1 407 3 383 2.29e-24 105
MS.gene067826.t1 AT5G61160 24.153 472 308 15 4 454 6 448 3.74e-24 105
MS.gene067826.t1 AT3G29680 24.160 476 308 15 4 458 6 449 4.24e-24 104
MS.gene067826.t1 AT5G07870 27.885 416 264 17 59 462 72 463 1.27e-23 103
MS.gene067826.t1 AT5G23970 23.937 447 287 18 2 440 1 402 1.80e-23 102
MS.gene067826.t1 AT4G31910 24.734 376 238 12 59 412 64 416 2.13e-23 102
MS.gene067826.t1 AT5G07860 27.792 403 244 17 59 449 72 439 2.60e-23 102
MS.gene067826.t1 AT3G50280 26.893 383 217 20 59 417 59 402 1.12e-22 100
MS.gene067826.t1 AT1G65450 24.762 315 197 9 152 456 1 285 1.82e-22 97.4
MS.gene067826.t1 AT3G50270 27.208 419 242 23 21 417 30 407 2.83e-22 99.4
MS.gene067826.t1 AT5G42830 25.307 407 257 18 59 449 64 439 4.04e-22 99.0
MS.gene067826.t1 AT5G07850 24.105 419 270 18 59 462 70 455 1.39e-21 97.4
MS.gene067826.t1 AT5G39090 25.157 477 296 20 6 457 8 448 7.44e-21 95.1
MS.gene067826.t1 AT5G67150 27.273 429 250 24 42 448 47 435 1.34e-20 94.4
MS.gene067826.t1 AT1G03940 24.236 491 297 21 6 457 11 465 1.13e-19 91.7
MS.gene067826.t1 AT5G47980 22.458 472 320 16 2 464 3 437 1.32e-19 91.3
MS.gene067826.t1 AT4G15400 24.262 474 301 19 2 464 3 429 3.87e-18 87.0
MS.gene067826.t1 AT3G50300 25.635 433 242 22 59 459 59 443 3.09e-17 84.3
MS.gene067826.t1 AT1G03495 26.023 342 210 14 6 318 11 338 6.18e-17 82.4
MS.gene067826.t1 AT3G50280 25.260 289 180 13 136 417 13 272 5.74e-16 79.0
MS.gene067826.t1 AT3G50290 25.214 234 152 9 136 362 19 236 2.13e-12 67.8
MS.gene067826.t1 AT3G23840 25.084 299 189 10 89 380 93 363 8.42e-11 64.3

Find 88 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGCCTAAGAATTATTT+TGG 0.081602 7.1:+19287611 MS.gene067826:CDS
CCGTTTAATGAGACCGATTT+TGG 0.124040 7.1:+19287887 MS.gene067826:CDS
GCTAAAAGAAATGGACAATT+TGG 0.222470 7.1:+19287824 MS.gene067826:CDS
GATATCACTTTAAAAGATTT+AGG 0.281321 7.1:+19287011 MS.gene067826:CDS
CTGGGTCGTTCTGTTCTTGA+TGG 0.283808 7.1:+19287167 MS.gene067826:CDS
AAGTGCGCTTTGAAGGAAAT+TGG 0.321861 7.1:+19287534 MS.gene067826:CDS
CTATGTATGAACGGTGGATT+TGG 0.326559 7.1:-19286713 None:intergenic
CCTTGTGGGTCACTCACTCT+TGG 0.327948 7.1:+19287137 MS.gene067826:CDS
TTCCCAACCACATAACAAAA+AGG 0.329036 7.1:-19287692 None:intergenic
ACTCACTCTTGGTCTTGCTC+TGG 0.329835 7.1:+19287148 MS.gene067826:CDS
TAAAGTTGGTATCCATGGTT+AGG 0.353132 7.1:-19286801 None:intergenic
CAACTTCATTGCAACAATAA+AGG 0.357124 7.1:+19286963 MS.gene067826:CDS
AGAAGGATATGAATTTGTTA+AGG 0.368085 7.1:+19287783 MS.gene067826:CDS
GCATTCCCAAAATAATTCTT+AGG 0.370343 7.1:-19287617 None:intergenic
AGGTTATTAGTTGTTACATA+TGG 0.371511 7.1:+19287498 MS.gene067826:CDS
AACAAAGGAACATCTTCAAC+TGG 0.377562 7.1:-19287092 None:intergenic
ACCAAGGTTGTTAACCTTGT+TGG 0.383376 7.1:-19287563 None:intergenic
TATATTCTTGATGAAGCTAA+TGG 0.388002 7.1:+19287971 MS.gene067826:CDS
CAGCCAACAACATACAAATT+CGG 0.390158 7.1:-19287854 None:intergenic
AGTTGAAGATGTTCCTTTGT+TGG 0.391503 7.1:+19287094 MS.gene067826:CDS
AGTGAGGGAAGCTATTAAAA+AGG 0.397885 7.1:+19287715 MS.gene067826:CDS
CCTGGTATTGTTAATTCTGA+TGG 0.403024 7.1:+19287935 MS.gene067826:CDS
CTGGTAGTTTCATTAGCTCA+TGG 0.408004 7.1:+19287198 MS.gene067826:CDS
AACAAAGTTCTATCATGAAA+TGG 0.413409 7.1:-19287263 None:intergenic
CCTTGGTTAATTGTAGAAAC+AGG 0.414948 7.1:+19287579 MS.gene067826:CDS
TTGACACTATCATCACAGTT+TGG 0.415059 7.1:-19288086 None:intergenic
GAAGGATATGAATTTGTTAA+GGG 0.417826 7.1:+19287784 MS.gene067826:CDS
GTTCTTGATGGTTCTTCTGC+TGG 0.417833 7.1:+19287179 MS.gene067826:CDS
AAACCTGATTGTATGGTTCC+TGG 0.419876 7.1:+19287917 MS.gene067826:CDS
ATCATGAAATGGAATCATAC+TGG 0.425406 7.1:-19287252 None:intergenic
ATAGTAGTGAGTGAGTGTTT+TGG 0.427197 7.1:-19286892 None:intergenic
ACTCACTACTATCCCTTTGC+AGG 0.430831 7.1:+19286903 MS.gene067826:CDS
CCAAAATCGGTCTCATTAAA+CGG 0.433375 7.1:-19287887 None:intergenic
AGTTTACCAAGTTTCCTTGT+GGG 0.443352 7.1:+19287123 MS.gene067826:CDS
CTCACTCTTGGTCTTGCTCT+GGG 0.445044 7.1:+19287149 MS.gene067826:CDS
ATTGTTAATTCTGATGGTAT+TGG 0.447032 7.1:+19287941 MS.gene067826:CDS
CGAATTTGTATGTTGTTGGC+TGG 0.452519 7.1:+19287855 MS.gene067826:CDS
GACCCGAATTTGTATGTTGT+TGG 0.455084 7.1:+19287851 MS.gene067826:CDS
GTTTGTAAAGTGCGCTTTGA+AGG 0.462639 7.1:+19287527 MS.gene067826:CDS
TGTTTGTGAAGAGGTAAAGT+TGG 0.465289 7.1:-19286815 None:intergenic
TAATTCTGATGGTATTGGTA+AGG 0.485819 7.1:+19287946 MS.gene067826:CDS
AGCCAACAACATACAAATTC+GGG 0.489178 7.1:-19287853 None:intergenic
CCAAAATAATTCTTAGGCAA+AGG 0.492931 7.1:-19287611 None:intergenic
GGTGAATTTCAACATGGTAG+TGG 0.502907 7.1:-19287402 None:intergenic
TGCAATGAAGTTGTAGTAAG+TGG 0.507183 7.1:-19286953 None:intergenic
CAGTTTACCAAGTTTCCTTG+TGG 0.509783 7.1:+19287122 MS.gene067826:CDS
TTATTAGTTGTTACATATGG+AGG 0.520920 7.1:+19287501 MS.gene067826:CDS
CCTTTGCAGGTAGATTAAGC+TGG 0.522403 7.1:+19286916 MS.gene067826:CDS
ACCAACAAGGTTAACAACCT+TGG 0.525311 7.1:+19287562 MS.gene067826:CDS
TGGTAGTTTCATTAGCTCAT+GGG 0.526316 7.1:+19287199 MS.gene067826:CDS
CCAGCTTAATCTACCTGCAA+AGG 0.526320 7.1:-19286916 None:intergenic
GTGGTTGGTGTTGTCTCTGA+AGG 0.528212 7.1:-19286741 None:intergenic
CCATCAGAATTAACAATACC+AGG 0.531351 7.1:-19287935 None:intergenic
GAGTGACCCACAAGGAAACT+TGG 0.531384 7.1:-19287129 None:intergenic
AAGAGATGAGTGATGATGAA+TGG 0.535532 7.1:-19286842 None:intergenic
AGAGGTAAAGTTGGTATCCA+TGG 0.536783 7.1:-19286806 None:intergenic
CAGCTTAATCTACCTGCAAA+GGG 0.537755 7.1:-19286915 None:intergenic
TCATGGGCTAAACTTGCTAA+AGG 0.538185 7.1:+19287215 MS.gene067826:CDS
ATACCAGGAACCATACAATC+AGG 0.550344 7.1:-19287920 None:intergenic
AAATAATTCTTAGGCAAAGG+TGG 0.552771 7.1:-19287608 None:intergenic
CTTGATGAAGCTAATGGCGA+TGG 0.560804 7.1:+19287977 MS.gene067826:CDS
AGGTTGTTAACCTTGTTGGT+TGG 0.562457 7.1:-19287559 None:intergenic
AAAGTTGGTATCCATGGTTA+GGG 0.563700 7.1:-19286800 None:intergenic
AACATGGTAGTGGCAAAGAG+TGG 0.563812 7.1:-19287392 None:intergenic
GAGCTTGAGTTGATCACAGA+GGG 0.580856 7.1:-19286778 None:intergenic
GAAAGAGAGAGTGTGGTGGT+TGG 0.587131 7.1:-19286756 None:intergenic
ACATGGTAGTGGCAAAGAGT+GGG 0.588001 7.1:-19287391 None:intergenic
ACAGAGGGAAAGAGAGAGTG+TGG 0.591568 7.1:-19286763 None:intergenic
TGATTATGTAGCAAATCAGA+AGG 0.592162 7.1:+19287766 MS.gene067826:CDS
GTTATGTGGTTGGGAAAGTG+AGG 0.594320 7.1:+19287699 MS.gene067826:CDS
CAACTCAAGCTCCCTAACCA+TGG 0.599984 7.1:+19286789 MS.gene067826:CDS
AAAAGAAATGGACAATTTGG+TGG 0.606770 7.1:+19287827 MS.gene067826:CDS
CCTGTTTCTACAATTAACCA+AGG 0.607399 7.1:-19287579 None:intergenic
GAAAACGTGGTGGTAAATGA+AGG 0.612694 7.1:-19287298 None:intergenic
GAACAAGTTTGTGTCACCGT+AGG 0.630705 7.1:-19287647 None:intergenic
GTAAACTGAACAACCAACAA+AGG 0.642328 7.1:-19287107 None:intergenic
AGCCAACACCAATGAAAACA+AGG 0.649428 7.1:+19287454 MS.gene067826:CDS
GAAATTGGAACCAACCAACA+AGG 0.652154 7.1:+19287549 MS.gene067826:CDS
ACAAAGGAACATCTTCAACT+GGG 0.664068 7.1:-19287091 None:intergenic
TTATGTGGTTGGGAAAGTGA+GGG 0.670881 7.1:+19287700 MS.gene067826:CDS
CCAAGAGTGAGTGACCCACA+AGG 0.679354 7.1:-19287137 None:intergenic
AAGGTGTAACTATGTATGAA+CGG 0.693375 7.1:-19286722 None:intergenic
ACCAATGAAAACAAGGTACG+AGG 0.701204 7.1:+19287461 MS.gene067826:CDS
GAGGGAAAGAGAGAGTGTGG+TGG 0.702222 7.1:-19286760 None:intergenic
AACTCATGATGATGAAAACG+TGG 0.716513 7.1:-19287311 None:intergenic
GTGTAACTATGTATGAACGG+TGG 0.717757 7.1:-19286719 None:intergenic
TCATGATGATGAAAACGTGG+TGG 0.758242 7.1:-19287308 None:intergenic
GGAGCTTGAGTTGATCACAG+AGG 0.762986 7.1:-19286779 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTGCTAAAAGAAA+TGG + chr7.1:19287815-19287834 MS.gene067826:CDS 15.0%
!!! AAGTTTTTCAACTTGTTTTT+TGG - chr7.1:19287426-19287445 None:intergenic 20.0%
!!! GATATCACTTTAAAAGATTT+AGG + chr7.1:19287011-19287030 MS.gene067826:CDS 20.0%
!!! TAATTTTTGGAACAAGTTTT+TGG - chr7.1:19287061-19287080 None:intergenic 20.0%
! AACAAAGTTCTATCATGAAA+TGG - chr7.1:19287266-19287285 None:intergenic 25.0%
! AGGTTATTAGTTGTTACATA+TGG + chr7.1:19287498-19287517 MS.gene067826:CDS 25.0%
! ATTGTTAATTCTGATGGTAT+TGG + chr7.1:19287941-19287960 MS.gene067826:CDS 25.0%
! TATATTCTTGATGAAGCTAA+TGG + chr7.1:19287971-19287990 MS.gene067826:CDS 25.0%
! TTATTAGTTGTTACATATGG+AGG + chr7.1:19287501-19287520 MS.gene067826:CDS 25.0%
!! ATTACTACTATTTTCCCAAA+GGG - chr7.1:19287348-19287367 None:intergenic 25.0%
!! CTTTGCCTAAGAATTATTTT+GGG + chr7.1:19287612-19287631 MS.gene067826:CDS 25.0%
!! TATTACTACTATTTTCCCAA+AGG - chr7.1:19287349-19287368 None:intergenic 25.0%
!!! AGAAGGATATGAATTTGTTA+AGG + chr7.1:19287783-19287802 MS.gene067826:CDS 25.0%
!!! GAAGGATATGAATTTGTTAA+GGG + chr7.1:19287784-19287803 MS.gene067826:CDS 25.0%
!!! TTTTAAAGTGATATCACATG+TGG - chr7.1:19287006-19287025 None:intergenic 25.0%
!!! TTTTTTGGTGAATTTCAACA+TGG - chr7.1:19287411-19287430 None:intergenic 25.0%
AAAAGAAATGGACAATTTGG+TGG + chr7.1:19287827-19287846 MS.gene067826:CDS 30.0%
AAATAATTCTTAGGCAAAGG+TGG - chr7.1:19287611-19287630 None:intergenic 30.0%
AAGGTGTAACTATGTATGAA+CGG - chr7.1:19286725-19286744 None:intergenic 30.0%
ATCATGAAATGGAATCATAC+TGG - chr7.1:19287255-19287274 None:intergenic 30.0%
CAACTTCATTGCAACAATAA+AGG + chr7.1:19286963-19286982 MS.gene067826:CDS 30.0%
CCAAAATAATTCTTAGGCAA+AGG - chr7.1:19287614-19287633 None:intergenic 30.0%
GCATTCCCAAAATAATTCTT+AGG - chr7.1:19287620-19287639 None:intergenic 30.0%
GCTAAAAGAAATGGACAATT+TGG + chr7.1:19287824-19287843 MS.gene067826:CDS 30.0%
TGATTATGTAGCAAATCAGA+AGG + chr7.1:19287766-19287785 MS.gene067826:CDS 30.0%
! CCTTTGCCTAAGAATTATTT+TGG + chr7.1:19287611-19287630 MS.gene067826:CDS 30.0%
! GGGACATCATAGTTAATTTT+TGG - chr7.1:19287074-19287093 None:intergenic 30.0%
! TTACTACTATTTTCCCAAAG+GGG - chr7.1:19287347-19287366 None:intergenic 30.0%
!! AATGGTTTTTGTTTGTGAAG+AGG - chr7.1:19286827-19286846 None:intergenic 30.0%
!! GAAGTTGTTTTATGAGGATA+TGG + chr7.1:19288048-19288067 MS.gene067826:CDS 30.0%
!! GTTGAAGAAGTTGTTTTATG+AGG + chr7.1:19288042-19288061 MS.gene067826:CDS 30.0%
!! TAATTCTGATGGTATTGGTA+AGG + chr7.1:19287946-19287965 MS.gene067826:CDS 30.0%
AACAAAGGAACATCTTCAAC+TGG - chr7.1:19287095-19287114 None:intergenic 35.0%
ACAAAGGAACATCTTCAACT+GGG - chr7.1:19287094-19287113 None:intergenic 35.0%
AGCCAACAACATACAAATTC+GGG - chr7.1:19287856-19287875 None:intergenic 35.0%
AGTGAGGGAAGCTATTAAAA+AGG + chr7.1:19287715-19287734 MS.gene067826:CDS 35.0%
AGTTGAAGATGTTCCTTTGT+TGG + chr7.1:19287094-19287113 MS.gene067826:CDS 35.0%
AGTTTACCAAGTTTCCTTGT+GGG + chr7.1:19287123-19287142 MS.gene067826:CDS 35.0%
CAGCCAACAACATACAAATT+CGG - chr7.1:19287857-19287876 None:intergenic 35.0%
CCAAAATCGGTCTCATTAAA+CGG - chr7.1:19287890-19287909 None:intergenic 35.0%
CCATCAGAATTAACAATACC+AGG - chr7.1:19287938-19287957 None:intergenic 35.0%
CCTGGTATTGTTAATTCTGA+TGG + chr7.1:19287935-19287954 MS.gene067826:CDS 35.0%
CCTTGGTTAATTGTAGAAAC+AGG + chr7.1:19287579-19287598 MS.gene067826:CDS 35.0%
GTAAACTGAACAACCAACAA+AGG - chr7.1:19287110-19287129 None:intergenic 35.0%
TGCAATGAAGTTGTAGTAAG+TGG - chr7.1:19286956-19286975 None:intergenic 35.0%
TGGTAGTTTCATTAGCTCAT+GGG + chr7.1:19287199-19287218 MS.gene067826:CDS 35.0%
TGTTTGTGAAGAGGTAAAGT+TGG - chr7.1:19286818-19286837 None:intergenic 35.0%
TTCCCAACCACATAACAAAA+AGG - chr7.1:19287695-19287714 None:intergenic 35.0%
! AACTCATGATGATGAAAACG+TGG - chr7.1:19287314-19287333 None:intergenic 35.0%
! AAGAGATGAGTGATGATGAA+TGG - chr7.1:19286845-19286864 None:intergenic 35.0%
! ATAGTAGTGAGTGAGTGTTT+TGG - chr7.1:19286895-19286914 None:intergenic 35.0%
!! AAAGTTGGTATCCATGGTTA+GGG - chr7.1:19286803-19286822 None:intergenic 35.0%
!! AAGCCTTTTTGTTATGTGGT+TGG + chr7.1:19287689-19287708 MS.gene067826:CDS 35.0%
!! AGCCTTTTTGTTATGTGGTT+GGG + chr7.1:19287690-19287709 MS.gene067826:CDS 35.0%
!! AGTTGTAGTAAGTGGTTTTG+TGG - chr7.1:19286948-19286967 None:intergenic 35.0%
!! CCTGTTTCTACAATTAACCA+AGG - chr7.1:19287582-19287601 None:intergenic 35.0%
!! CGTTTAATGAGACCGATTTT+GGG + chr7.1:19287888-19287907 MS.gene067826:CDS 35.0%
!! GAACAAGCCTTTTTGTTATG+TGG + chr7.1:19287685-19287704 MS.gene067826:CDS 35.0%
!! TAAAGTTGGTATCCATGGTT+AGG - chr7.1:19286804-19286823 None:intergenic 35.0%
!!! ACGAGGTTATACTAGTTTTG+AGG + chr7.1:19287478-19287497 MS.gene067826:CDS 35.0%
!!! TACCTTGTTTTCATTGGTGT+TGG - chr7.1:19287459-19287478 None:intergenic 35.0%
AAACCTGATTGTATGGTTCC+TGG + chr7.1:19287917-19287936 MS.gene067826:CDS 40.0%
ACCAACAAGGTTAACAACCT+TGG + chr7.1:19287562-19287581 MS.gene067826:CDS 40.0%
ACCAAGGTTGTTAACCTTGT+TGG - chr7.1:19287566-19287585 None:intergenic 40.0%
ACCAATGAAAACAAGGTACG+AGG + chr7.1:19287461-19287480 MS.gene067826:CDS 40.0%
AGCCAACACCAATGAAAACA+AGG + chr7.1:19287454-19287473 MS.gene067826:CDS 40.0%
AGGTTGTTAACCTTGTTGGT+TGG - chr7.1:19287562-19287581 None:intergenic 40.0%
ATACCAGGAACCATACAATC+AGG - chr7.1:19287923-19287942 None:intergenic 40.0%
CAGCTTAATCTACCTGCAAA+GGG - chr7.1:19286918-19286937 None:intergenic 40.0%
CAGTTTACCAAGTTTCCTTG+TGG + chr7.1:19287122-19287141 MS.gene067826:CDS 40.0%
CGAATTTGTATGTTGTTGGC+TGG + chr7.1:19287855-19287874 MS.gene067826:CDS 40.0%
CTGGTAGTTTCATTAGCTCA+TGG + chr7.1:19287198-19287217 MS.gene067826:CDS 40.0%
GAAAACGTGGTGGTAAATGA+AGG - chr7.1:19287301-19287320 None:intergenic 40.0%
GAAATTGGAACCAACCAACA+AGG + chr7.1:19287549-19287568 MS.gene067826:CDS 40.0%
GACCCGAATTTGTATGTTGT+TGG + chr7.1:19287851-19287870 MS.gene067826:CDS 40.0%
GGTGAATTTCAACATGGTAG+TGG - chr7.1:19287405-19287424 None:intergenic 40.0%
GTGTAACTATGTATGAACGG+TGG - chr7.1:19286722-19286741 None:intergenic 40.0%
GTTTGTAAAGTGCGCTTTGA+AGG + chr7.1:19287527-19287546 MS.gene067826:CDS 40.0%
TTATGTGGTTGGGAAAGTGA+GGG + chr7.1:19287700-19287719 MS.gene067826:CDS 40.0%
! CTATGTATGAACGGTGGATT+TGG - chr7.1:19286716-19286735 None:intergenic 40.0%
! TCATGATGATGAAAACGTGG+TGG - chr7.1:19287311-19287330 None:intergenic 40.0%
! TGGGAATGCAACTTTTCCTA+CGG + chr7.1:19287631-19287650 MS.gene067826:CDS 40.0%
!! AAGTGCGCTTTGAAGGAAAT+TGG + chr7.1:19287534-19287553 MS.gene067826:CDS 40.0%
!! AGAGGTAAAGTTGGTATCCA+TGG - chr7.1:19286809-19286828 None:intergenic 40.0%
!! CCGTTTAATGAGACCGATTT+TGG + chr7.1:19287887-19287906 MS.gene067826:CDS 40.0%
!! TAATGAGACCGATTTTGGGT+GGG + chr7.1:19287892-19287911 MS.gene067826:CDS 40.0%
!! TCATGGGCTAAACTTGCTAA+AGG + chr7.1:19287215-19287234 MS.gene067826:CDS 40.0%
!! TTAATGAGACCGATTTTGGG+TGG + chr7.1:19287891-19287910 MS.gene067826:CDS 40.0%
!!! ACCTCGTACCTTGTTTTCAT+TGG - chr7.1:19287465-19287484 None:intergenic 40.0%
ACTCACTACTATCCCTTTGC+AGG + chr7.1:19286903-19286922 MS.gene067826:CDS 45.0%
CCAGCTTAATCTACCTGCAA+AGG - chr7.1:19286919-19286938 None:intergenic 45.0%
CCTTTGCAGGTAGATTAAGC+TGG + chr7.1:19286916-19286935 MS.gene067826:CDS 45.0%
CTTGATGAAGCTAATGGCGA+TGG + chr7.1:19287977-19287996 MS.gene067826:CDS 45.0%
GAACAAGTTTGTGTCACCGT+AGG - chr7.1:19287650-19287669 None:intergenic 45.0%
GTTATGTGGTTGGGAAAGTG+AGG + chr7.1:19287699-19287718 MS.gene067826:CDS 45.0%
! CTACTATTTTCCCAAAGGGG+TGG - chr7.1:19287344-19287363 None:intergenic 45.0%
! GTTCTTGATGGTTCTTCTGC+TGG + chr7.1:19287179-19287198 MS.gene067826:CDS 45.0%
!! AACATGGTAGTGGCAAAGAG+TGG - chr7.1:19287395-19287414 None:intergenic 45.0%
!! AATGAGACCGATTTTGGGTG+GGG + chr7.1:19287893-19287912 MS.gene067826:CDS 45.0%
!! ACATGGTAGTGGCAAAGAGT+GGG - chr7.1:19287394-19287413 None:intergenic 45.0%
!! GAGCTTGAGTTGATCACAGA+GGG - chr7.1:19286781-19286800 None:intergenic 45.0%
!!! AGTGAGTGTTTTGGAGAGAG+AGG - chr7.1:19286886-19286905 None:intergenic 45.0%
ACAGAGGGAAAGAGAGAGTG+TGG - chr7.1:19286766-19286785 None:intergenic 50.0%
AGGTTTCCCCCACCCAAAAT+CGG - chr7.1:19287903-19287922 None:intergenic 50.0%
CAACTCAAGCTCCCTAACCA+TGG + chr7.1:19286789-19286808 MS.gene067826:CDS 50.0%
CTGGGTCGTTCTGTTCTTGA+TGG + chr7.1:19287167-19287186 MS.gene067826:CDS 50.0%
GAGTGACCCACAAGGAAACT+TGG - chr7.1:19287132-19287151 None:intergenic 50.0%
GTGGGGGAAACCTGATTGTA+TGG + chr7.1:19287910-19287929 MS.gene067826:CDS 50.0%
! AGTTTTCTCCACCACCCCTT+TGG + chr7.1:19287330-19287349 MS.gene067826:CDS 50.0%
! CTATTTTCCCAAAGGGGTGG+TGG - chr7.1:19287341-19287360 None:intergenic 50.0%
! GAAAGAGAGAGTGTGGTGGT+TGG - chr7.1:19286759-19286778 None:intergenic 50.0%
! GTTTTCTCCACCACCCCTTT+GGG + chr7.1:19287331-19287350 MS.gene067826:CDS 50.0%
!! ACTCACTCTTGGTCTTGCTC+TGG + chr7.1:19287148-19287167 MS.gene067826:CDS 50.0%
!! ATGAGACCGATTTTGGGTGG+GGG + chr7.1:19287894-19287913 MS.gene067826:CDS 50.0%
!! CTCACTCTTGGTCTTGCTCT+GGG + chr7.1:19287149-19287168 MS.gene067826:CDS 50.0%
!! GGAGCTTGAGTTGATCACAG+AGG - chr7.1:19286782-19286801 None:intergenic 50.0%
!! GTGGTTGGTGTTGTCTCTGA+AGG - chr7.1:19286744-19286763 None:intergenic 50.0%
CCAAGAGTGAGTGACCCACA+AGG - chr7.1:19287140-19287159 None:intergenic 55.0%
CCTTGTGGGTCACTCACTCT+TGG + chr7.1:19287137-19287156 MS.gene067826:CDS 55.0%
! GAGGGAAAGAGAGAGTGTGG+TGG - chr7.1:19286763-19286782 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 19286702 19288096 19286702 ID=MS.gene067826
chr7.1 mRNA 19286702 19288096 19286702 ID=MS.gene067826.t1;Parent=MS.gene067826
chr7.1 exon 19286702 19288096 19286702 ID=MS.gene067826.t1.exon1;Parent=MS.gene067826.t1
chr7.1 CDS 19286702 19288096 19286702 ID=cds.MS.gene067826.t1;Parent=MS.gene067826.t1
Gene Sequence

>MS.gene067826

ATGGTGAACATCCAAATCCACCGTTCATACATAGTTACACCTTCAGAGACAACACCAACCACCACACTCTCTCTTTCCCTCTGTGATCAACTCAAGCTCCCTAACCATGGATACCAACTTTACCTCTTCACAAACAAAAACCATTCATCATCACTCATCTCTTCAACAAACATTCTCATAACCTCTCTCTCCAAAACACTCACTCACTACTATCCCTTTGCAGGTAGATTAAGCTGGATCAAACCACAAAACCACTTACTACAACTTCATTGCAACAATAAAGGTGTACAATTCTTAGAAGCCACATGTGATATCACTTTAAAAGATTTAGGTAGTTTTGATTCTACACATGTTGTCCAAAAACTTGTTCCAAAAATTAACTATGATGTCCCAGTTGAAGATGTTCCTTTGTTGGTTGTTCAGTTTACCAAGTTTCCTTGTGGGTCACTCACTCTTGGTCTTGCTCTGGGTCGTTCTGTTCTTGATGGTTCTTCTGCTGGTAGTTTCATTAGCTCATGGGCTAAACTTGCTAAAGGTGAGAGTTTAGATTCCAGTATGATTCCATTTCATGATAGAACTTTGTTAGATTCAAAGATCCTTCATTTACCACCACGTTTTCATCATCATGAGTTTTCTCCACCACCCCTTTGGGAAAATAGTAGTAATAACTCAACAATTGTGAGTAAAAACCCACTCTTTGCCACTACCATGTTGAAATTCACCAAAAAACAAGTTGAAAAACTTAAGAACAAAGCCAACACCAATGAAAACAAGGTACGAGGTTATACTAGTTTTGAGGTTATTAGTTGTTACATATGGAGGTGTGTTTGTAAAGTGCGCTTTGAAGGAAATTGGAACCAACCAACAAGGTTAACAACCTTGGTTAATTGTAGAAACAGGTTGAATCCACCTTTGCCTAAGAATTATTTTGGGAATGCAACTTTTCCTACGGTGACACAAACTTGTTCATTTGATGATGTTGTGAACAAGCCTTTTTGTTATGTGGTTGGGAAAGTGAGGGAAGCTATTAAAAAGGTGAATGATGAGTATGTAAGATCTGTTCTTGATTATGTAGCAAATCAGAAGGATATGAATTTGTTAAGGGACAAGTTTTATAATTTTGCTAAAAGAAATGGACAATTTGGTGGTGACCCGAATTTGTATGTTGTTGGCTGGACTAATTTTCCGTTTAATGAGACCGATTTTGGGTGGGGGAAACCTGATTGTATGGTTCCTGGTATTGTTAATTCTGATGGTATTGGTAAGGCTTATATTCTTGATGAAGCTAATGGCGATGGCTTTGTTGTAAGTGTTTGTTTGCAATCTTTTCATATAGATGCGTTGAAGAAGTTGTTTTATGAGGATATGGAGATGATCACTAGTTCCAAACTGTGA
Protein sequence

>MS.gene067826.t1

MVNIQIHRSYIVTPSETTPTTTLSLSLCDQLKLPNHGYQLYLFTNKNHSSSLISSTNILITSLSKTLTHYYPFAGRLSWIKPQNHLLQLHCNNKGVQFLEATCDITLKDLGSFDSTHVVQKLVPKINYDVPVEDVPLLVVQFTKFPCGSLTLGLALGRSVLDGSSAGSFISSWAKLAKGESLDSSMIPFHDRTLLDSKILHLPPRFHHHEFSPPPLWENSSNNSTIVSKNPLFATTMLKFTKKQVEKLKNKANTNENKVRGYTSFEVISCYIWRCVCKVRFEGNWNQPTRLTTLVNCRNRLNPPLPKNYFGNATFPTVTQTCSFDDVVNKPFCYVVGKVREAIKKVNDEYVRSVLDYVANQKDMNLLRDKFYNFAKRNGQFGGDPNLYVVGWTNFPFNETDFGWGKPDCMVPGIVNSDGIGKAYILDEANGDGFVVSVCLQSFHIDALKKLFYEDMEMITSSKL