Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068008.t1 | XP_003608370.3 | 93.8 | 243 | 8 | 1 | 1 | 236 | 32 | 274 | 3.60E-114 | 421 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068008.t1 | Q9LM24 | 53.7 | 82 | 38 | 0 | 95 | 176 | 189 | 270 | 5.6e-15 | 82.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068008.t1 | B0F6T0 | 93.8 | 242 | 8 | 1 | 1 | 235 | 197 | 438 | 7.6e-114 | 419.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene068008 | 0.810502 | 1.12E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene068008 | 0.821011 | 5.06E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene068008 | 0.830444 | 2.93E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene068008 | 0.807284 | 5.45E-50 | -1.69E-46 |
| MS.gene053597 | MS.gene068008 | 0.957013 | 7.53E-115 | -1.69E-46 |
| MS.gene053599 | MS.gene068008 | 0.970053 | 4.90E-131 | -1.69E-46 |
| MS.gene055465 | MS.gene068008 | 0.820294 | 7.40E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene068008 | 0.813946 | 1.98E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene068008 | 0.815963 | 7.06E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene068008 | 0.815669 | 8.21E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene068008 | 0.831199 | 1.91E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene068008 | 0.806146 | 9.48E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068008.t1 | MTR_4g093150 | 92.958 | 213 | 8 | 1 | 31 | 236 | 1 | 213 | 8.40e-140 | 390 |
| MS.gene068008.t1 | MTR_2g009770 | 52.903 | 155 | 72 | 1 | 61 | 214 | 98 | 252 | 4.65e-36 | 129 |
| MS.gene068008.t1 | MTR_1g054835 | 36.585 | 123 | 76 | 1 | 78 | 198 | 42 | 164 | 3.65e-13 | 68.6 |
| MS.gene068008.t1 | MTR_1g070350 | 34.167 | 120 | 77 | 1 | 73 | 192 | 509 | 626 | 2.13e-12 | 66.6 |
| MS.gene068008.t1 | MTR_5g078300 | 38.750 | 80 | 49 | 0 | 91 | 170 | 612 | 691 | 1.22e-11 | 64.3 |
| MS.gene068008.t1 | MTR_4g107310 | 31.250 | 208 | 122 | 7 | 43 | 234 | 262 | 464 | 3.38e-11 | 62.8 |
| MS.gene068008.t1 | MTR_0168s0060 | 33.333 | 132 | 85 | 1 | 75 | 203 | 307 | 438 | 6.06e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068008.t1 | AT5G16720 | 37.000 | 100 | 63 | 0 | 99 | 198 | 360 | 459 | 3.72e-15 | 74.7 |
| MS.gene068008.t1 | AT1G70750 | 34.783 | 115 | 72 | 1 | 96 | 207 | 410 | 524 | 2.91e-14 | 72.0 |
| MS.gene068008.t1 | AT1G74830 | 37.500 | 96 | 60 | 0 | 99 | 194 | 305 | 400 | 3.37e-14 | 71.6 |
| MS.gene068008.t1 | AT1G18265 | 52.273 | 88 | 42 | 0 | 89 | 176 | 183 | 270 | 4.87e-14 | 70.1 |
| MS.gene068008.t1 | AT5G06560 | 35.000 | 100 | 65 | 0 | 99 | 198 | 74 | 173 | 2.48e-12 | 66.2 |
Find 53 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCCCATCTTTGGAAAA+TGG | 0.168151 | 4.3:-21048620 | MS.gene068008:CDS |
| TGAGTCGAAGAATTGGTATT+AGG | 0.240330 | 4.3:+21048590 | None:intergenic |
| TCCCACAATTCGTTTATGTT+TGG | 0.317458 | 4.3:-21048672 | MS.gene068008:CDS |
| GAAGAACTCAAGCAATATCT+TGG | 0.374861 | 4.3:-21048188 | MS.gene068008:CDS |
| GAGTCGAAGAATTGGTATTA+GGG | 0.386407 | 4.3:+21048591 | None:intergenic |
| TGATGAAGTGATCGAGTCTT+TGG | 0.389665 | 4.3:-21048276 | MS.gene068008:CDS |
| AAATCCCATCTTTGGAAAAT+GGG | 0.392542 | 4.3:-21048619 | MS.gene068008:CDS |
| GAAAGCTAATGCGGCGTTTG+CGG | 0.398717 | 4.3:-21048441 | MS.gene068008:CDS |
| AAGAACTCAAGCAATATCTT+GGG | 0.416257 | 4.3:-21048187 | MS.gene068008:CDS |
| TCTCTTCTGAGTCGAAGAAT+TGG | 0.418771 | 4.3:+21048583 | None:intergenic |
| GAAGAGATTGAATGGTCGTT+TGG | 0.421960 | 4.3:-21048567 | MS.gene068008:CDS |
| ATGTTTGGAAAACTTTGTTT+TGG | 0.423857 | 4.3:-21048657 | MS.gene068008:CDS |
| GTGTGTTGGAAGAGCAGTTG+AGG | 0.428235 | 4.3:-21048220 | MS.gene068008:CDS |
| TTCCAAACATAAACGAATTG+TGG | 0.432068 | 4.3:+21048670 | None:intergenic |
| TCCAAACATAAACGAATTGT+GGG | 0.444313 | 4.3:+21048671 | None:intergenic |
| AAGCAATATCTTGGGGAGGA+TGG | 0.455312 | 4.3:-21048179 | MS.gene068008:CDS |
| AGGCAATGGCGATGATTCTC+AGG | 0.455510 | 4.3:-21048376 | MS.gene068008:CDS |
| TGAATGGTCGTTTGGAAGAT+CGG | 0.461435 | 4.3:-21048559 | MS.gene068008:CDS |
| GGCTTCTTCATCTGCGGAAG+AGG | 0.462933 | 4.3:-21048396 | MS.gene068008:CDS |
| GTAGTTAGCTCTTCTGAAAA+CGG | 0.463530 | 4.3:-21048092 | MS.gene068008:CDS |
| GGCGTTTGCGGAGCTTGATA+AGG | 0.464641 | 4.3:-21048429 | MS.gene068008:CDS |
| TCAAGATTCTTCACTTCGGT+TGG | 0.466081 | 4.3:-21048739 | MS.gene068008:CDS |
| TCTCGAGGCATGAATCTCAT+AGG | 0.486643 | 4.3:-21048244 | MS.gene068008:CDS |
| TGTGTTGGAAGAGCAGTTGA+GGG | 0.487289 | 4.3:-21048219 | MS.gene068008:CDS |
| AGCAATATCTTGGGGAGGAT+GGG | 0.488013 | 4.3:-21048178 | MS.gene068008:CDS |
| AAGCTGATGTTAGTAGAGAT+AGG | 0.499821 | 4.3:-21048142 | MS.gene068008:CDS |
| GAAAGATGATAGTGAAATGC+AGG | 0.502055 | 4.3:-21048528 | MS.gene068008:CDS |
| TCGACTCAGAAGAGATTGAA+TGG | 0.516087 | 4.3:-21048575 | MS.gene068008:CDS |
| TTCATCTGCGGAAGAGGCAA+TGG | 0.519311 | 4.3:-21048390 | MS.gene068008:CDS |
| TACTAACATCAGCTTCAAGT+TGG | 0.521972 | 4.3:+21048150 | None:intergenic |
| CACAACCTTGGAAACTTACA+AGG | 0.522214 | 4.3:+21048061 | None:intergenic |
| AGCGAATCAATTCCGCAGAA+TGG | 0.525489 | 4.3:-21048321 | MS.gene068008:CDS |
| TGGTCGTTTGGAAGATCGGT+TGG | 0.527702 | 4.3:-21048555 | MS.gene068008:CDS |
| TCAAACACCTCATCCACATC+AGG | 0.540118 | 4.3:+21048503 | None:intergenic |
| TGTTTCAAGATTCTTCACTT+CGG | 0.555363 | 4.3:-21048743 | MS.gene068008:CDS |
| AAGTGATCGAGTCTTTGGAA+TGG | 0.572333 | 4.3:-21048271 | MS.gene068008:CDS |
| TGAATCTCATAGGAGTGTGT+TGG | 0.573968 | 4.3:-21048234 | MS.gene068008:CDS |
| GTTTGGAAGATCGGTTGGAA+AGG | 0.591359 | 4.3:-21048550 | MS.gene068008:CDS |
| TTGCGGAGCTTGATAAGGAA+CGG | 0.610513 | 4.3:-21048424 | MS.gene068008:CDS |
| GCAGGTTCCTGATGTGGATG+AGG | 0.610761 | 4.3:-21048510 | MS.gene068008:CDS |
| CTTTGGAATGGACGATCTCG+AGG | 0.612708 | 4.3:-21048259 | MS.gene068008:CDS |
| AGTTGATAAAGATTGAGCGC+AGG | 0.614343 | 4.3:-21048463 | MS.gene068008:CDS |
| ACCTTGGAAACTTACAAGGT+CGG | 0.615530 | 4.3:+21048065 | None:intergenic |
| ACTTCTGTTCAGCCATTCTG+CGG | 0.620830 | 4.3:+21048309 | None:intergenic |
| TGAAATGCAGGTTCCTGATG+TGG | 0.621860 | 4.3:-21048516 | MS.gene068008:CDS |
| AAAATCACAGAGAAAACGTA+GGG | 0.635297 | 4.3:+21048703 | None:intergenic |
| GCTTCAGAGTGAGAAGAGCT+CGG | 0.636966 | 4.3:-21048354 | MS.gene068008:CDS |
| GCTTGATAAGGAACGGACAG+CGG | 0.649607 | 4.3:-21048417 | MS.gene068008:CDS |
| TGAGCGCAGGAAAGCTAATG+CGG | 0.653423 | 4.3:-21048450 | MS.gene068008:CDS |
| AGAACTCAAGCAATATCTTG+GGG | 0.655002 | 4.3:-21048186 | MS.gene068008:CDS |
| ACTCAAGCAATATCTTGGGG+AGG | 0.657753 | 4.3:-21048183 | MS.gene068008:CDS |
| GACAGCGGCTTCTTCATCTG+CGG | 0.673445 | 4.3:-21048402 | MS.gene068008:CDS |
| CAAAATCACAGAGAAAACGT+AGG | 0.688372 | 4.3:+21048702 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAGAAAAAAATCCCATCTT+TGG | - | chr4.3:21048213-21048232 | MS.gene068008:CDS | 25.0% |
| !!! | ATGTTTGGAAAACTTTGTTT+TGG | - | chr4.3:21048183-21048202 | MS.gene068008:CDS | 25.0% |
| AAAATCACAGAGAAAACGTA+GGG | + | chr4.3:21048140-21048159 | None:intergenic | 30.0% | |
| AAGAACTCAAGCAATATCTT+GGG | - | chr4.3:21048653-21048672 | MS.gene068008:CDS | 30.0% | |
| TCCAAACATAAACGAATTGT+GGG | + | chr4.3:21048172-21048191 | None:intergenic | 30.0% | |
| TGTTTCAAGATTCTTCACTT+CGG | - | chr4.3:21048097-21048116 | MS.gene068008:CDS | 30.0% | |
| TTCCAAACATAAACGAATTG+TGG | + | chr4.3:21048173-21048192 | None:intergenic | 30.0% | |
| ! | AAAATCCCATCTTTGGAAAA+TGG | - | chr4.3:21048220-21048239 | MS.gene068008:CDS | 30.0% |
| ! | AAATCCCATCTTTGGAAAAT+GGG | - | chr4.3:21048221-21048240 | MS.gene068008:CDS | 30.0% |
| AAGCTGATGTTAGTAGAGAT+AGG | - | chr4.3:21048698-21048717 | MS.gene068008:CDS | 35.0% | |
| AGAACTCAAGCAATATCTTG+GGG | - | chr4.3:21048654-21048673 | MS.gene068008:CDS | 35.0% | |
| CAAAATCACAGAGAAAACGT+AGG | + | chr4.3:21048141-21048160 | None:intergenic | 35.0% | |
| GAAGAACTCAAGCAATATCT+TGG | - | chr4.3:21048652-21048671 | MS.gene068008:CDS | 35.0% | |
| GAGTCGAAGAATTGGTATTA+GGG | + | chr4.3:21048252-21048271 | None:intergenic | 35.0% | |
| TACTAACATCAGCTTCAAGT+TGG | + | chr4.3:21048693-21048712 | None:intergenic | 35.0% | |
| TCCCACAATTCGTTTATGTT+TGG | - | chr4.3:21048168-21048187 | MS.gene068008:CDS | 35.0% | |
| TGAGTCGAAGAATTGGTATT+AGG | + | chr4.3:21048253-21048272 | None:intergenic | 35.0% | |
| ! | CTACGTTTTCTCTGTGATTT+TGG | - | chr4.3:21048139-21048158 | MS.gene068008:CDS | 35.0% |
| ! | GAAAGATGATAGTGAAATGC+AGG | - | chr4.3:21048312-21048331 | MS.gene068008:CDS | 35.0% |
| ! | GTAGTTAGCTCTTCTGAAAA+CGG | - | chr4.3:21048748-21048767 | MS.gene068008:CDS | 35.0% |
| GAAGAGATTGAATGGTCGTT+TGG | - | chr4.3:21048273-21048292 | MS.gene068008:CDS | 40.0% | |
| TCAAGATTCTTCACTTCGGT+TGG | - | chr4.3:21048101-21048120 | MS.gene068008:CDS | 40.0% | |
| TCGACTCAGAAGAGATTGAA+TGG | - | chr4.3:21048265-21048284 | MS.gene068008:CDS | 40.0% | |
| TCTCTTCTGAGTCGAAGAAT+TGG | + | chr4.3:21048260-21048279 | None:intergenic | 40.0% | |
| TGAATGGTCGTTTGGAAGAT+CGG | - | chr4.3:21048281-21048300 | MS.gene068008:CDS | 40.0% | |
| TGATGAAGTGATCGAGTCTT+TGG | - | chr4.3:21048564-21048583 | MS.gene068008:CDS | 40.0% | |
| ! | AAGTGATCGAGTCTTTGGAA+TGG | - | chr4.3:21048569-21048588 | MS.gene068008:CDS | 40.0% |
| ! | CACTTCGGTTGGCATTTTAA+GGG | - | chr4.3:21048112-21048131 | MS.gene068008:CDS | 40.0% |
| ! | GAGACCCATTTTCCAAAGAT+GGG | + | chr4.3:21048228-21048247 | None:intergenic | 40.0% |
| ! | TCACTTCGGTTGGCATTTTA+AGG | - | chr4.3:21048111-21048130 | MS.gene068008:CDS | 40.0% |
| ! | TGAATCTCATAGGAGTGTGT+TGG | - | chr4.3:21048606-21048625 | MS.gene068008:CDS | 40.0% |
| ! | TGAGACCCATTTTCCAAAGA+TGG | + | chr4.3:21048229-21048248 | None:intergenic | 40.0% |
| !! | AGTTGATAAAGATTGAGCGC+AGG | - | chr4.3:21048377-21048396 | MS.gene068008:CDS | 40.0% |
| AAGCAATATCTTGGGGAGGA+TGG | - | chr4.3:21048661-21048680 | MS.gene068008:CDS | 45.0% | |
| ACTCAAGCAATATCTTGGGG+AGG | - | chr4.3:21048657-21048676 | MS.gene068008:CDS | 45.0% | |
| ACTTCTGTTCAGCCATTCTG+CGG | + | chr4.3:21048534-21048553 | None:intergenic | 45.0% | |
| AGCAATATCTTGGGGAGGAT+GGG | - | chr4.3:21048662-21048681 | MS.gene068008:CDS | 45.0% | |
| AGCGAATCAATTCCGCAGAA+TGG | - | chr4.3:21048519-21048538 | MS.gene068008:CDS | 45.0% | |
| GTTTGGAAGATCGGTTGGAA+AGG | - | chr4.3:21048290-21048309 | MS.gene068008:CDS | 45.0% | |
| TCAAACACCTCATCCACATC+AGG | + | chr4.3:21048340-21048359 | None:intergenic | 45.0% | |
| TCTCGAGGCATGAATCTCAT+AGG | - | chr4.3:21048596-21048615 | MS.gene068008:CDS | 45.0% | |
| TGAAATGCAGGTTCCTGATG+TGG | - | chr4.3:21048324-21048343 | MS.gene068008:CDS | 45.0% | |
| TGTGTTGGAAGAGCAGTTGA+GGG | - | chr4.3:21048621-21048640 | MS.gene068008:CDS | 45.0% | |
| TTGCGGAGCTTGATAAGGAA+CGG | - | chr4.3:21048416-21048435 | MS.gene068008:CDS | 45.0% | |
| AGGCAATGGCGATGATTCTC+AGG | - | chr4.3:21048464-21048483 | MS.gene068008:CDS | 50.0% | |
| GAAAGCTAATGCGGCGTTTG+CGG | - | chr4.3:21048399-21048418 | MS.gene068008:CDS | 50.0% | |
| GCTTCAGAGTGAGAAGAGCT+CGG | - | chr4.3:21048486-21048505 | MS.gene068008:CDS | 50.0% | |
| GCTTGATAAGGAACGGACAG+CGG | - | chr4.3:21048423-21048442 | MS.gene068008:CDS | 50.0% | |
| GTGTGTTGGAAGAGCAGTTG+AGG | - | chr4.3:21048620-21048639 | MS.gene068008:CDS | 50.0% | |
| TGAGCGCAGGAAAGCTAATG+CGG | - | chr4.3:21048390-21048409 | MS.gene068008:CDS | 50.0% | |
| TGGTCGTTTGGAAGATCGGT+TGG | - | chr4.3:21048285-21048304 | MS.gene068008:CDS | 50.0% | |
| TTCATCTGCGGAAGAGGCAA+TGG | - | chr4.3:21048450-21048469 | MS.gene068008:CDS | 50.0% | |
| !! | CTTTGGAATGGACGATCTCG+AGG | - | chr4.3:21048581-21048600 | MS.gene068008:CDS | 50.0% |
| GACAGCGGCTTCTTCATCTG+CGG | - | chr4.3:21048438-21048457 | MS.gene068008:CDS | 55.0% | |
| GCAGGTTCCTGATGTGGATG+AGG | - | chr4.3:21048330-21048349 | MS.gene068008:CDS | 55.0% | |
| GGCGTTTGCGGAGCTTGATA+AGG | - | chr4.3:21048411-21048430 | MS.gene068008:CDS | 55.0% | |
| GGCTTCTTCATCTGCGGAAG+AGG | - | chr4.3:21048444-21048463 | MS.gene068008:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 21048076 | 21048786 | 21048076 | ID=MS.gene068008 |
| chr4.3 | mRNA | 21048076 | 21048786 | 21048076 | ID=MS.gene068008.t1;Parent=MS.gene068008 |
| chr4.3 | exon | 21048076 | 21048786 | 21048076 | ID=MS.gene068008.t1.exon1;Parent=MS.gene068008.t1 |
| chr4.3 | CDS | 21048076 | 21048786 | 21048076 | ID=cds.MS.gene068008.t1;Parent=MS.gene068008.t1 |
| Gene Sequence |
| Protein sequence |
