AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053597


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053597.t1 MTR_4g093150 92.958 213 8 1 31 236 1 213 8.40e-140 390
MS.gene053597.t1 MTR_2g009770 52.903 155 72 1 61 214 98 252 4.65e-36 129
MS.gene053597.t1 MTR_1g054835 36.585 123 76 1 78 198 42 164 3.65e-13 68.6
MS.gene053597.t1 MTR_1g070350 34.167 120 77 1 73 192 509 626 2.13e-12 66.6
MS.gene053597.t1 MTR_5g078300 38.750 80 49 0 91 170 612 691 1.22e-11 64.3
MS.gene053597.t1 MTR_4g107310 31.250 208 122 7 43 234 262 464 3.38e-11 62.8
MS.gene053597.t1 MTR_0168s0060 33.333 132 85 1 75 203 307 438 6.06e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053597.t1 AT5G16720 37.000 100 63 0 99 198 360 459 3.72e-15 74.7
MS.gene053597.t1 AT1G70750 34.783 115 72 1 96 207 410 524 2.91e-14 72.0
MS.gene053597.t1 AT1G74830 37.500 96 60 0 99 194 305 400 3.37e-14 71.6
MS.gene053597.t1 AT1G18265 52.273 88 42 0 89 176 183 270 4.87e-14 70.1
MS.gene053597.t1 AT5G06560 35.000 100 65 0 99 198 74 173 2.48e-12 66.2

Find 53 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCCATCTTTGGAAAA+TGG 0.168151 4.4:-21536227 MS.gene053597:CDS
TGAGTCGAAGAATTGGTATT+AGG 0.240330 4.4:+21536197 None:intergenic
TCCCACAATTCGTTTATGTT+TGG 0.317458 4.4:-21536279 MS.gene053597:CDS
GAAGAACTCAAGCAATATCT+TGG 0.374861 4.4:-21535795 MS.gene053597:CDS
GAGTCGAAGAATTGGTATTA+GGG 0.386407 4.4:+21536198 None:intergenic
TGATGAAGTGATCGAGTCTT+TGG 0.389665 4.4:-21535883 MS.gene053597:CDS
AAATCCCATCTTTGGAAAAT+GGG 0.392542 4.4:-21536226 MS.gene053597:CDS
GAAAGCTAATGCGGCGTTTG+CGG 0.398717 4.4:-21536048 MS.gene053597:CDS
AAGAACTCAAGCAATATCTT+GGG 0.416257 4.4:-21535794 MS.gene053597:CDS
TCTCTTCTGAGTCGAAGAAT+TGG 0.418771 4.4:+21536190 None:intergenic
GAAGAGATTGAATGGTCGTT+TGG 0.421960 4.4:-21536174 MS.gene053597:CDS
ATGTTTGGAAAACTTTGTTT+TGG 0.423857 4.4:-21536264 MS.gene053597:CDS
GTGTGTTGGAAGAGCAGTTG+AGG 0.428235 4.4:-21535827 MS.gene053597:CDS
TTCCAAACATAAACGAATTG+TGG 0.432068 4.4:+21536277 None:intergenic
TCCAAACATAAACGAATTGT+GGG 0.444313 4.4:+21536278 None:intergenic
AAGCAATATCTTGGGGAGGA+TGG 0.455312 4.4:-21535786 MS.gene053597:CDS
AGGCAATGGCGATGATTCTC+AGG 0.455510 4.4:-21535983 MS.gene053597:CDS
TGAATGGTCGTTTGGAAGAT+CGG 0.461435 4.4:-21536166 MS.gene053597:CDS
GGCTTCTTCATCTGCGGAAG+AGG 0.462933 4.4:-21536003 MS.gene053597:CDS
GTAGTTAGCTCTTCTGAAAA+CGG 0.463530 4.4:-21535699 MS.gene053597:CDS
GGCGTTTGCGGAGCTTGATA+AGG 0.464641 4.4:-21536036 MS.gene053597:CDS
TCAAGATTCTTCACTTCGGT+TGG 0.466081 4.4:-21536346 MS.gene053597:CDS
TCTCGAGGCATGAATCTCAT+AGG 0.486643 4.4:-21535851 MS.gene053597:CDS
TGTGTTGGAAGAGCAGTTGA+GGG 0.487289 4.4:-21535826 MS.gene053597:CDS
AGCAATATCTTGGGGAGGAT+GGG 0.488013 4.4:-21535785 MS.gene053597:CDS
AAGCTGATGTTAGTAGAGAT+AGG 0.499821 4.4:-21535749 MS.gene053597:CDS
GAAAGATGATAGTGAAATGC+AGG 0.502055 4.4:-21536135 MS.gene053597:CDS
TCGACTCAGAAGAGATTGAA+TGG 0.516087 4.4:-21536182 MS.gene053597:CDS
TTCATCTGCGGAAGAGGCAA+TGG 0.519311 4.4:-21535997 MS.gene053597:CDS
TACTAACATCAGCTTCAAGT+TGG 0.521972 4.4:+21535757 None:intergenic
CACAACCTTGGAAACTTACA+AGG 0.522214 4.4:+21535668 None:intergenic
AGCGAATCAATTCCGCAGAA+TGG 0.525489 4.4:-21535928 MS.gene053597:CDS
TGGTCGTTTGGAAGATCGGT+TGG 0.527702 4.4:-21536162 MS.gene053597:CDS
TCAAACACCTCATCCACATC+AGG 0.540118 4.4:+21536110 None:intergenic
TGTTTCAAGATTCTTCACTT+CGG 0.555363 4.4:-21536350 MS.gene053597:CDS
AAGTGATCGAGTCTTTGGAA+TGG 0.572333 4.4:-21535878 MS.gene053597:CDS
TGAATCTCATAGGAGTGTGT+TGG 0.573968 4.4:-21535841 MS.gene053597:CDS
GTTTGGAAGATCGGTTGGAA+AGG 0.591359 4.4:-21536157 MS.gene053597:CDS
TTGCGGAGCTTGATAAGGAA+CGG 0.610513 4.4:-21536031 MS.gene053597:CDS
GCAGGTTCCTGATGTGGATG+AGG 0.610761 4.4:-21536117 MS.gene053597:CDS
CTTTGGAATGGACGATCTCG+AGG 0.612708 4.4:-21535866 MS.gene053597:CDS
AGTTGATAAAGATTGAGCGC+AGG 0.614343 4.4:-21536070 MS.gene053597:CDS
ACCTTGGAAACTTACAAGGT+CGG 0.615530 4.4:+21535672 None:intergenic
ACTTCTGTTCAGCCATTCTG+CGG 0.620830 4.4:+21535916 None:intergenic
TGAAATGCAGGTTCCTGATG+TGG 0.621860 4.4:-21536123 MS.gene053597:CDS
AAAATCACAGAGAAAACGTA+GGG 0.635297 4.4:+21536310 None:intergenic
GCTTCAGAGTGAGAAGAGCT+CGG 0.636966 4.4:-21535961 MS.gene053597:CDS
GCTTGATAAGGAACGGACAG+CGG 0.649607 4.4:-21536024 MS.gene053597:CDS
TGAGCGCAGGAAAGCTAATG+CGG 0.653423 4.4:-21536057 MS.gene053597:CDS
AGAACTCAAGCAATATCTTG+GGG 0.655002 4.4:-21535793 MS.gene053597:CDS
ACTCAAGCAATATCTTGGGG+AGG 0.657753 4.4:-21535790 MS.gene053597:CDS
GACAGCGGCTTCTTCATCTG+CGG 0.673445 4.4:-21536009 MS.gene053597:CDS
CAAAATCACAGAGAAAACGT+AGG 0.688372 4.4:+21536309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAGAAAAAAATCCCATCTT+TGG - chr4.4:21535820-21535839 MS.gene053597:CDS 25.0%
!!! ATGTTTGGAAAACTTTGTTT+TGG - chr4.4:21535790-21535809 MS.gene053597:CDS 25.0%
AAAATCACAGAGAAAACGTA+GGG + chr4.4:21535747-21535766 None:intergenic 30.0%
AAGAACTCAAGCAATATCTT+GGG - chr4.4:21536260-21536279 MS.gene053597:CDS 30.0%
TCCAAACATAAACGAATTGT+GGG + chr4.4:21535779-21535798 None:intergenic 30.0%
TGTTTCAAGATTCTTCACTT+CGG - chr4.4:21535704-21535723 MS.gene053597:CDS 30.0%
TTCCAAACATAAACGAATTG+TGG + chr4.4:21535780-21535799 None:intergenic 30.0%
! AAAATCCCATCTTTGGAAAA+TGG - chr4.4:21535827-21535846 MS.gene053597:CDS 30.0%
! AAATCCCATCTTTGGAAAAT+GGG - chr4.4:21535828-21535847 MS.gene053597:CDS 30.0%
AAGCTGATGTTAGTAGAGAT+AGG - chr4.4:21536305-21536324 MS.gene053597:CDS 35.0%
AGAACTCAAGCAATATCTTG+GGG - chr4.4:21536261-21536280 MS.gene053597:CDS 35.0%
CAAAATCACAGAGAAAACGT+AGG + chr4.4:21535748-21535767 None:intergenic 35.0%
GAAGAACTCAAGCAATATCT+TGG - chr4.4:21536259-21536278 MS.gene053597:CDS 35.0%
GAGTCGAAGAATTGGTATTA+GGG + chr4.4:21535859-21535878 None:intergenic 35.0%
TACTAACATCAGCTTCAAGT+TGG + chr4.4:21536300-21536319 None:intergenic 35.0%
TCCCACAATTCGTTTATGTT+TGG - chr4.4:21535775-21535794 MS.gene053597:CDS 35.0%
TGAGTCGAAGAATTGGTATT+AGG + chr4.4:21535860-21535879 None:intergenic 35.0%
! CTACGTTTTCTCTGTGATTT+TGG - chr4.4:21535746-21535765 MS.gene053597:CDS 35.0%
! GAAAGATGATAGTGAAATGC+AGG - chr4.4:21535919-21535938 MS.gene053597:CDS 35.0%
! GTAGTTAGCTCTTCTGAAAA+CGG - chr4.4:21536355-21536374 MS.gene053597:CDS 35.0%
GAAGAGATTGAATGGTCGTT+TGG - chr4.4:21535880-21535899 MS.gene053597:CDS 40.0%
TCAAGATTCTTCACTTCGGT+TGG - chr4.4:21535708-21535727 MS.gene053597:CDS 40.0%
TCGACTCAGAAGAGATTGAA+TGG - chr4.4:21535872-21535891 MS.gene053597:CDS 40.0%
TCTCTTCTGAGTCGAAGAAT+TGG + chr4.4:21535867-21535886 None:intergenic 40.0%
TGAATGGTCGTTTGGAAGAT+CGG - chr4.4:21535888-21535907 MS.gene053597:CDS 40.0%
TGATGAAGTGATCGAGTCTT+TGG - chr4.4:21536171-21536190 MS.gene053597:CDS 40.0%
! AAGTGATCGAGTCTTTGGAA+TGG - chr4.4:21536176-21536195 MS.gene053597:CDS 40.0%
! CACTTCGGTTGGCATTTTAA+GGG - chr4.4:21535719-21535738 MS.gene053597:CDS 40.0%
! GAGACCCATTTTCCAAAGAT+GGG + chr4.4:21535835-21535854 None:intergenic 40.0%
! TCACTTCGGTTGGCATTTTA+AGG - chr4.4:21535718-21535737 MS.gene053597:CDS 40.0%
! TGAATCTCATAGGAGTGTGT+TGG - chr4.4:21536213-21536232 MS.gene053597:CDS 40.0%
! TGAGACCCATTTTCCAAAGA+TGG + chr4.4:21535836-21535855 None:intergenic 40.0%
!! AGTTGATAAAGATTGAGCGC+AGG - chr4.4:21535984-21536003 MS.gene053597:CDS 40.0%
AAGCAATATCTTGGGGAGGA+TGG - chr4.4:21536268-21536287 MS.gene053597:CDS 45.0%
ACTCAAGCAATATCTTGGGG+AGG - chr4.4:21536264-21536283 MS.gene053597:CDS 45.0%
ACTTCTGTTCAGCCATTCTG+CGG + chr4.4:21536141-21536160 None:intergenic 45.0%
AGCAATATCTTGGGGAGGAT+GGG - chr4.4:21536269-21536288 MS.gene053597:CDS 45.0%
AGCGAATCAATTCCGCAGAA+TGG - chr4.4:21536126-21536145 MS.gene053597:CDS 45.0%
GTTTGGAAGATCGGTTGGAA+AGG - chr4.4:21535897-21535916 MS.gene053597:CDS 45.0%
TCAAACACCTCATCCACATC+AGG + chr4.4:21535947-21535966 None:intergenic 45.0%
TCTCGAGGCATGAATCTCAT+AGG - chr4.4:21536203-21536222 MS.gene053597:CDS 45.0%
TGAAATGCAGGTTCCTGATG+TGG - chr4.4:21535931-21535950 MS.gene053597:CDS 45.0%
TGTGTTGGAAGAGCAGTTGA+GGG - chr4.4:21536228-21536247 MS.gene053597:CDS 45.0%
TTGCGGAGCTTGATAAGGAA+CGG - chr4.4:21536023-21536042 MS.gene053597:CDS 45.0%
AGGCAATGGCGATGATTCTC+AGG - chr4.4:21536071-21536090 MS.gene053597:CDS 50.0%
GAAAGCTAATGCGGCGTTTG+CGG - chr4.4:21536006-21536025 MS.gene053597:CDS 50.0%
GCTTCAGAGTGAGAAGAGCT+CGG - chr4.4:21536093-21536112 MS.gene053597:CDS 50.0%
GCTTGATAAGGAACGGACAG+CGG - chr4.4:21536030-21536049 MS.gene053597:CDS 50.0%
GTGTGTTGGAAGAGCAGTTG+AGG - chr4.4:21536227-21536246 MS.gene053597:CDS 50.0%
TGAGCGCAGGAAAGCTAATG+CGG - chr4.4:21535997-21536016 MS.gene053597:CDS 50.0%
TGGTCGTTTGGAAGATCGGT+TGG - chr4.4:21535892-21535911 MS.gene053597:CDS 50.0%
TTCATCTGCGGAAGAGGCAA+TGG - chr4.4:21536057-21536076 MS.gene053597:CDS 50.0%
!! CTTTGGAATGGACGATCTCG+AGG - chr4.4:21536188-21536207 MS.gene053597:CDS 50.0%
GACAGCGGCTTCTTCATCTG+CGG - chr4.4:21536045-21536064 MS.gene053597:CDS 55.0%
GCAGGTTCCTGATGTGGATG+AGG - chr4.4:21535937-21535956 MS.gene053597:CDS 55.0%
GGCGTTTGCGGAGCTTGATA+AGG - chr4.4:21536018-21536037 MS.gene053597:CDS 55.0%
GGCTTCTTCATCTGCGGAAG+AGG - chr4.4:21536051-21536070 MS.gene053597:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 21535683 21536393 21535683 ID=MS.gene053597
chr4.4 mRNA 21535683 21536393 21535683 ID=MS.gene053597.t1;Parent=MS.gene053597
chr4.4 exon 21535683 21536393 21535683 ID=MS.gene053597.t1.exon1;Parent=MS.gene053597.t1
chr4.4 CDS 21535683 21536393 21535683 ID=cds.MS.gene053597.t1;Parent=MS.gene053597.t1
Gene Sequence

>MS.gene053597

ATGTTTTTGATGTTAGGCTTTTGTTTCAAGATTCTTCACTTCGGTTGGCATTTTAAGGGTGCCCTACGTTTTCTCTGTGATTTTGGACGCATTCCCACAATTCGTTTATGTTTGGAAAACTTTGTTTTGGAAGTTTCTAAGAAAAAAATCCCATCTTTGGAAAATGGGTCTCACCCTAATACCAATTCTTCGACTCAGAAGAGATTGAATGGTCGTTTGGAAGATCGGTTGGAAAGGAAAGATGATAGTGAAATGCAGGTTCCTGATGTGGATGAGGTGTTTGATGTAATCACTCTGAGAAAGTTGATAAAGATTGAGCGCAGGAAAGCTAATGCGGCGTTTGCGGAGCTTGATAAGGAACGGACAGCGGCTTCTTCATCTGCGGAAGAGGCAATGGCGATGATTCTCAGGCTTCAGAGTGAGAAGAGCTCGGCTGAAATTCAAGCGAATCAATTCCGCAGAATGGCTGAACAGAAGTTAGAGTATGATGATGAAGTGATCGAGTCTTTGGAATGGACGATCTCGAGGCATGAATCTCATAGGAGTGTGTTGGAAGAGCAGTTGAGGGTTTATAGAGAAGAACTCAAGCAATATCTTGGGGAGGATGGGATAAACCAACTTGAAGCTGATGTTAGTAGAGATAGGAGTTTTGAGAATGAAGCAGTTGATTCTGTAGTTAGCTCTTCTGAAAACGGATCACCGACCTTGTAA

Protein sequence

>MS.gene053597.t1

MFLMLGFCFKILHFGWHFKGALRFLCDFGRIPTIRLCLENFVLEVSKKKIPSLENGSHPNTNSSTQKRLNGRLEDRLERKDDSEMQVPDVDEVFDVITLRKLIKIERRKANAAFAELDKERTAASSSAEEAMAMILRLQSEKSSAEIQANQFRRMAEQKLEYDDEVIESLEWTISRHESHRSVLEEQLRVYREELKQYLGEDGINQLEADVSRDRSFENEAVDSVVSSSENGSPTL