Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068034.t1 | XP_003608424.1 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 3.00E-06 | 61.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068034.t1 | G7JV73 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 2.2e-06 | 61.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene068034 | 0.841051 | 6.01E-58 | -1.69E-46 |
| MS.gene053568 | MS.gene068034 | 0.935147 | 1.34E-96 | -1.69E-46 |
| MS.gene053569 | MS.gene068034 | 0.912138 | 2.74E-83 | -1.69E-46 |
| MS.gene058068 | MS.gene068034 | 0.814041 | 1.89E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068034.t1 | MTR_4g093940 | 92.969 | 128 | 4 | 1 | 1 | 128 | 1 | 123 | 2.55e-66 | 196 |
| MS.gene068034.t1 | MTR_4g093950 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 1.11e-49 | 155 |
| MS.gene068034.t1 | MTR_4g093910 | 92.857 | 112 | 8 | 0 | 1 | 112 | 1 | 112 | 8.66e-49 | 152 |
| MS.gene068034.t1 | MTR_4g093920 | 91.743 | 109 | 8 | 1 | 1 | 109 | 1 | 108 | 1.72e-47 | 149 |
| MS.gene068034.t1 | MTR_5g064530 | 64.474 | 152 | 28 | 4 | 1 | 128 | 1 | 150 | 1.06e-40 | 132 |
| MS.gene068034.t1 | MTR_5g064510 | 66.667 | 123 | 21 | 4 | 1 | 105 | 1 | 121 | 4.68e-36 | 120 |
| MS.gene068034.t1 | MTR_4g094678 | 69.620 | 79 | 6 | 3 | 17 | 92 | 1 | 64 | 2.17e-22 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTTCCTTGAGGCATATT+TGG | 0.193132 | 4.3:-20786857 | None:intergenic |
| GTGGGAATGGTAGGTAAATT+TGG | 0.271860 | 4.3:-20786794 | None:intergenic |
| GCAATAGATGGCGAGCTTCT+AGG | 0.294792 | 4.3:-20786757 | None:intergenic |
| GGTGGTAATGTAGTTGGTTT+TGG | 0.298989 | 4.3:-20786821 | None:intergenic |
| TAAAGCTGCAATGAAGGATT+TGG | 0.355352 | 4.3:-20786705 | None:intergenic |
| AGATGGAATTGTTGGAATAT+TGG | 0.374933 | 4.3:-20786945 | None:intergenic |
| GTGTTGTTGTTTGCAATAGA+TGG | 0.376065 | 4.3:-20786769 | None:intergenic |
| GGGAAAGTGGTTGGGATTGA+AGG | 0.382492 | 4.3:-20787013 | None:intergenic |
| GGCTAGGAGTTGAAGGTGGT+GGG | 0.405223 | 4.3:-20787054 | None:intergenic |
| TGAGGCATATTTGGAATTGA+AGG | 0.405314 | 4.3:-20786848 | None:intergenic |
| ATAGTGAGTTTAGGCATAGA+AGG | 0.436994 | 4.3:-20786899 | None:intergenic |
| GGGAAGGAGGGGAAAGTGGT+TGG | 0.439412 | 4.3:-20787022 | None:intergenic |
| GGAAGGAGGGGAAAGTGGTT+GGG | 0.444157 | 4.3:-20787021 | None:intergenic |
| GGCAAAGGTGGTAATGTAGT+TGG | 0.444412 | 4.3:-20786827 | None:intergenic |
| TCTAGAAGTGCTTAAGGGCT+AGG | 0.453177 | 4.3:-20787070 | None:intergenic |
| GGTTGGGATTGAAGGAAGGT+TGG | 0.454159 | 4.3:-20787005 | None:intergenic |
| AGTGGTGGCATAGTGAGTTT+AGG | 0.457061 | 4.3:-20786908 | None:intergenic |
| AAATGGGAAGGAGGGGAAAG+TGG | 0.467197 | 4.3:-20787026 | None:intergenic |
| GCTTAAGGGCTAGGAGTTGA+AGG | 0.469112 | 4.3:-20787061 | None:intergenic |
| GTTGGGATTGAAGGAAGGTT+GGG | 0.469467 | 4.3:-20787004 | None:intergenic |
| TTAGGCATAGAAGGGATAGT+AGG | 0.471087 | 4.3:-20786890 | None:intergenic |
| GGGCTAGGAGTTGAAGGTGG+TGG | 0.483282 | 4.3:-20787055 | None:intergenic |
| AAGAAGTAAAGCTGCAATGA+AGG | 0.485067 | 4.3:-20786711 | None:intergenic |
| GGAATATTGGTAGGAAGACT+TGG | 0.503031 | 4.3:-20786932 | None:intergenic |
| AAGTGGTTGGGATTGAAGGA+AGG | 0.508086 | 4.3:-20787009 | None:intergenic |
| TGCAATGAAGGATTTGGTTG+AGG | 0.510590 | 4.3:-20786699 | None:intergenic |
| TTTGGTAAAGTGGGAATGGT+AGG | 0.519398 | 4.3:-20786803 | None:intergenic |
| AGTGAAGTGGCTGCTGGCAA+TGG | 0.520284 | 4.3:-20786980 | None:intergenic |
| GGAATTGTTGGAATATTGGT+AGG | 0.523994 | 4.3:-20786941 | None:intergenic |
| ATGTTGAGAGATGGAATTGT+TGG | 0.529497 | 4.3:-20786953 | None:intergenic |
| TTGGTAGGAAGACTTGGAAG+TGG | 0.530626 | 4.3:-20786926 | None:intergenic |
| GAAGGGATAGTAGGCAATGG+AGG | 0.560608 | 4.3:-20786881 | None:intergenic |
| GAAGTGGCTGCTGGCAATGG+TGG | 0.580562 | 4.3:-20786977 | None:intergenic |
| TTTGGAATTGAAGGCAAAGG+TGG | 0.580888 | 4.3:-20786839 | None:intergenic |
| TTGGGAAGTGAAGTGGCTGC+TGG | 0.584437 | 4.3:-20786986 | None:intergenic |
| TCAATTCCAAATATGCCTCA+AGG | 0.589431 | 4.3:+20786851 | MS.gene068034:CDS |
| GCTAGGAGTTGAAGGTGGTG+GGG | 0.597019 | 4.3:-20787053 | None:intergenic |
| GTAGGAAGACTTGGAAGTGG+TGG | 0.611131 | 4.3:-20786923 | None:intergenic |
| ATATTTGGAATTGAAGGCAA+AGG | 0.615183 | 4.3:-20786842 | None:intergenic |
| TAGTGAGTTTAGGCATAGAA+GGG | 0.616158 | 4.3:-20786898 | None:intergenic |
| ATAGAAGGGATAGTAGGCAA+TGG | 0.624968 | 4.3:-20786884 | None:intergenic |
| AGGAAGGTTGGGAAGTGAAG+TGG | 0.630070 | 4.3:-20786993 | None:intergenic |
| AATGGTGGAATGTTGAGAGA+TGG | 0.653205 | 4.3:-20786962 | None:intergenic |
| AATGGAGGAAGATTTCCTTG+AGG | 0.717776 | 4.3:-20786866 | None:intergenic |
| TAAGGGCTAGGAGTTGAAGG+TGG | 0.721449 | 4.3:-20787058 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGAATTGTTGGAATAT+TGG | - | chr4.3:20786948-20786967 | None:intergenic | 30.0% | |
| ! | ATATTTGGAATTGAAGGCAA+AGG | - | chr4.3:20786845-20786864 | None:intergenic | 30.0% |
| AAGAAGTAAAGCTGCAATGA+AGG | - | chr4.3:20786714-20786733 | None:intergenic | 35.0% | |
| AGATTTCCTTGAGGCATATT+TGG | - | chr4.3:20786860-20786879 | None:intergenic | 35.0% | |
| ATAGTGAGTTTAGGCATAGA+AGG | - | chr4.3:20786902-20786921 | None:intergenic | 35.0% | |
| ATGTTGAGAGATGGAATTGT+TGG | - | chr4.3:20786956-20786975 | None:intergenic | 35.0% | |
| GGAATTGTTGGAATATTGGT+AGG | - | chr4.3:20786944-20786963 | None:intergenic | 35.0% | |
| TAAAGCTGCAATGAAGGATT+TGG | - | chr4.3:20786708-20786727 | None:intergenic | 35.0% | |
| TAGTGAGTTTAGGCATAGAA+GGG | - | chr4.3:20786901-20786920 | None:intergenic | 35.0% | |
| TCAATTCCAAATATGCCTCA+AGG | + | chr4.3:20786851-20786870 | MS.gene068034:CDS | 35.0% | |
| ! | TGAGGCATATTTGGAATTGA+AGG | - | chr4.3:20786851-20786870 | None:intergenic | 35.0% |
| !! | GTGTTGTTGTTTGCAATAGA+TGG | - | chr4.3:20786772-20786791 | None:intergenic | 35.0% |
| !!! | GTAGTTGGTTTTGGTAAAGT+GGG | - | chr4.3:20786815-20786834 | None:intergenic | 35.0% |
| !!! | TGTAGTTGGTTTTGGTAAAG+TGG | - | chr4.3:20786816-20786835 | None:intergenic | 35.0% |
| AATGGAGGAAGATTTCCTTG+AGG | - | chr4.3:20786869-20786888 | None:intergenic | 40.0% | |
| ATAGAAGGGATAGTAGGCAA+TGG | - | chr4.3:20786887-20786906 | None:intergenic | 40.0% | |
| GGAATATTGGTAGGAAGACT+TGG | - | chr4.3:20786935-20786954 | None:intergenic | 40.0% | |
| GTGGGAATGGTAGGTAAATT+TGG | - | chr4.3:20786797-20786816 | None:intergenic | 40.0% | |
| TTAGGCATAGAAGGGATAGT+AGG | - | chr4.3:20786893-20786912 | None:intergenic | 40.0% | |
| TTTGGTAAAGTGGGAATGGT+AGG | - | chr4.3:20786806-20786825 | None:intergenic | 40.0% | |
| ! | AATGGTGGAATGTTGAGAGA+TGG | - | chr4.3:20786965-20786984 | None:intergenic | 40.0% |
| ! | TGCAATGAAGGATTTGGTTG+AGG | - | chr4.3:20786702-20786721 | None:intergenic | 40.0% |
| ! | TTTGGAATTGAAGGCAAAGG+TGG | - | chr4.3:20786842-20786861 | None:intergenic | 40.0% |
| !! | AAGGTGGTGGGGAAAAAAAT+GGG | - | chr4.3:20787045-20787064 | None:intergenic | 40.0% |
| !! | GGTGGTAATGTAGTTGGTTT+TGG | - | chr4.3:20786824-20786843 | None:intergenic | 40.0% |
| !!! | TGGTTTTGGTAAAGTGGGAA+TGG | - | chr4.3:20786810-20786829 | None:intergenic | 40.0% |
| AAGTGGTTGGGATTGAAGGA+AGG | - | chr4.3:20787012-20787031 | None:intergenic | 45.0% | |
| AGTGGTGGCATAGTGAGTTT+AGG | - | chr4.3:20786911-20786930 | None:intergenic | 45.0% | |
| GGGAAAAAAATGGGAAGGAG+GGG | - | chr4.3:20787036-20787055 | None:intergenic | 45.0% | |
| GGGGAAAAAAATGGGAAGGA+GGG | - | chr4.3:20787037-20787056 | None:intergenic | 45.0% | |
| GTTGGGATTGAAGGAAGGTT+GGG | - | chr4.3:20787007-20787026 | None:intergenic | 45.0% | |
| TGGGGAAAAAAATGGGAAGG+AGG | - | chr4.3:20787038-20787057 | None:intergenic | 45.0% | |
| TGGTGGGGAAAAAAATGGGA+AGG | - | chr4.3:20787041-20787060 | None:intergenic | 45.0% | |
| TTGGTAGGAAGACTTGGAAG+TGG | - | chr4.3:20786929-20786948 | None:intergenic | 45.0% | |
| ! | GGCAAAGGTGGTAATGTAGT+TGG | - | chr4.3:20786830-20786849 | None:intergenic | 45.0% |
| !! | GAAGGTGGTGGGGAAAAAAA+TGG | - | chr4.3:20787046-20787065 | None:intergenic | 45.0% |
| AAATGGGAAGGAGGGGAAAG+TGG | - | chr4.3:20787029-20787048 | None:intergenic | 50.0% | |
| AGGAAGGTTGGGAAGTGAAG+TGG | - | chr4.3:20786996-20787015 | None:intergenic | 50.0% | |
| GAAGGGATAGTAGGCAATGG+AGG | - | chr4.3:20786884-20786903 | None:intergenic | 50.0% | |
| GCAATAGATGGCGAGCTTCT+AGG | - | chr4.3:20786760-20786779 | None:intergenic | 50.0% | |
| GGGAAAGTGGTTGGGATTGA+AGG | - | chr4.3:20787016-20787035 | None:intergenic | 50.0% | |
| GGTTGGGATTGAAGGAAGGT+TGG | - | chr4.3:20787008-20787027 | None:intergenic | 50.0% | |
| GTAGGAAGACTTGGAAGTGG+TGG | - | chr4.3:20786926-20786945 | None:intergenic | 50.0% | |
| TAAGGGCTAGGAGTTGAAGG+TGG | - | chr4.3:20787061-20787080 | None:intergenic | 50.0% | |
| AGTGAAGTGGCTGCTGGCAA+TGG | - | chr4.3:20786983-20787002 | None:intergenic | 55.0% | |
| GCTAGGAGTTGAAGGTGGTG+GGG | - | chr4.3:20787056-20787075 | None:intergenic | 55.0% | |
| GGAAGGAGGGGAAAGTGGTT+GGG | - | chr4.3:20787024-20787043 | None:intergenic | 55.0% | |
| GGCTAGGAGTTGAAGGTGGT+GGG | - | chr4.3:20787057-20787076 | None:intergenic | 55.0% | |
| TTGGGAAGTGAAGTGGCTGC+TGG | - | chr4.3:20786989-20787008 | None:intergenic | 55.0% | |
| GAAGTGGCTGCTGGCAATGG+TGG | - | chr4.3:20786980-20786999 | None:intergenic | 60.0% | |
| GGGAAGGAGGGGAAAGTGGT+TGG | - | chr4.3:20787025-20787044 | None:intergenic | 60.0% | |
| GGGCTAGGAGTTGAAGGTGG+TGG | - | chr4.3:20787058-20787077 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20786695 | 20787081 | 20786695 | ID=MS.gene068034 |
| chr4.3 | mRNA | 20786695 | 20787081 | 20786695 | ID=MS.gene068034.t1;Parent=MS.gene068034 |
| chr4.3 | exon | 20786695 | 20787081 | 20786695 | ID=MS.gene068034.t1.exon1;Parent=MS.gene068034.t1 |
| chr4.3 | CDS | 20786695 | 20787081 | 20786695 | ID=cds.MS.gene068034.t1;Parent=MS.gene068034.t1 |
| Gene Sequence |
| Protein sequence |
