Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053569.t1 | XP_003608424.1 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 3.00E-06 | 61.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053569.t1 | G7JV73 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 2.2e-06 | 61.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene053569 | 0.854227 | 1.42E-61 | -1.69E-46 |
| MS.gene053568 | MS.gene053569 | 0.936446 | 1.71E-97 | -1.69E-46 |
| MS.gene053569 | MS.gene057717 | 0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene053569 | MS.gene061044 | 0.803862 | 2.85E-49 | -1.69E-46 |
| MS.gene053569 | MS.gene068034 | 0.912138 | 2.74E-83 | -1.69E-46 |
| MS.gene053569 | MS.gene068035 | 0.931429 | 3.83E-94 | -1.69E-46 |
| MS.gene053569 | MS.gene068037 | 0.831703 | 1.44E-55 | -1.69E-46 |
| MS.gene053569 | MS.gene09819 | 0.815382 | 9.51E-52 | -1.69E-46 |
| MS.gene053569 | MS.gene39959 | 0.816644 | 4.97E-52 | -1.69E-46 |
| MS.gene053569 | MS.gene44966 | 0.811598 | 6.46E-51 | -1.69E-46 |
| MS.gene053569 | MS.gene50534 | 0.8176 | 3.03E-52 | -1.69E-46 |
| MS.gene053569 | MS.gene57291 | 0.800228 | 1.59E-48 | -1.69E-46 |
| MS.gene053569 | MS.gene58425 | 0.805815 | 1.11E-49 | -1.69E-46 |
| MS.gene053569 | MS.gene63578 | 0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene053569 | MS.gene64069 | 0.858132 | 1.02E-62 | -1.69E-46 |
| MS.gene053569 | MS.gene76883 | 0.814046 | 1.88E-51 | -1.69E-46 |
| MS.gene053569 | MS.gene78768 | 0.821612 | 3.68E-53 | -1.69E-46 |
| MS.gene053569 | MS.gene90209 | 0.937304 | 4.31E-98 | -1.69E-46 |
| MS.gene053569 | MS.gene90210 | 0.950354 | 1.96E-108 | -1.69E-46 |
| MS.gene053569 | MS.gene90969 | 0.832716 | 8.06E-56 | -1.69E-46 |
| MS.gene053569 | MS.gene91317 | 0.829855 | 4.07E-55 | -1.69E-46 |
| MS.gene053569 | MS.gene96701 | 0.824868 | 6.39E-54 | -1.69E-46 |
| MS.gene053569 | MS.gene99127 | 0.804101 | 2.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053569.t1 | MTR_4g093940 | 92.969 | 128 | 4 | 1 | 1 | 128 | 1 | 123 | 2.55e-66 | 196 |
| MS.gene053569.t1 | MTR_4g093950 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 1.11e-49 | 155 |
| MS.gene053569.t1 | MTR_4g093910 | 92.857 | 112 | 8 | 0 | 1 | 112 | 1 | 112 | 8.66e-49 | 152 |
| MS.gene053569.t1 | MTR_4g093920 | 91.743 | 109 | 8 | 1 | 1 | 109 | 1 | 108 | 1.72e-47 | 149 |
| MS.gene053569.t1 | MTR_5g064530 | 64.474 | 152 | 28 | 4 | 1 | 128 | 1 | 150 | 1.06e-40 | 132 |
| MS.gene053569.t1 | MTR_5g064510 | 66.667 | 123 | 21 | 4 | 1 | 105 | 1 | 121 | 4.68e-36 | 120 |
| MS.gene053569.t1 | MTR_4g094678 | 69.620 | 79 | 6 | 3 | 17 | 92 | 1 | 64 | 2.17e-22 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTTCCTTGAGGCATATT+TGG | 0.193132 | 4.4:-21278892 | None:intergenic |
| GTGGGAATGGTAGGTAAATT+TGG | 0.271860 | 4.4:-21278829 | None:intergenic |
| GCAATAGATGGCGAGCTTCT+AGG | 0.294792 | 4.4:-21278792 | None:intergenic |
| GGTGGCAATGTTGTTGGTTT+TGG | 0.324240 | 4.4:-21278856 | None:intergenic |
| TAAAGCTGCAATGAAGGATT+TGG | 0.355352 | 4.4:-21278740 | None:intergenic |
| AGATGGAATTGTTGGAATAT+TGG | 0.374933 | 4.4:-21278980 | None:intergenic |
| GTGTTGTTGTTTGCAATAGA+TGG | 0.376065 | 4.4:-21278804 | None:intergenic |
| GGGAAAGTGGTTGGGATTGA+AGG | 0.382492 | 4.4:-21279048 | None:intergenic |
| GGCTAGGAGTTGAAGGTGGT+GGG | 0.405223 | 4.4:-21279089 | None:intergenic |
| TGAGGCATATTTGGAATTGA+AGG | 0.405314 | 4.4:-21278883 | None:intergenic |
| ATAGTGAGTTTAGGCATAGA+AGG | 0.436994 | 4.4:-21278934 | None:intergenic |
| GGGAAGGAGGGGAAAGTGGT+TGG | 0.439412 | 4.4:-21279057 | None:intergenic |
| GGAAGGAGGGGAAAGTGGTT+GGG | 0.444157 | 4.4:-21279056 | None:intergenic |
| TCTAGAAGTGCTTAAGGGCT+AGG | 0.453177 | 4.4:-21279105 | None:intergenic |
| GGTTGGGATTGAAGGAAGGT+TGG | 0.454159 | 4.4:-21279040 | None:intergenic |
| AGTGGTGGCATAGTGAGTTT+AGG | 0.457061 | 4.4:-21278943 | None:intergenic |
| AAATGGGAAGGAGGGGAAAG+TGG | 0.467197 | 4.4:-21279061 | None:intergenic |
| GCTTAAGGGCTAGGAGTTGA+AGG | 0.469112 | 4.4:-21279096 | None:intergenic |
| GTTGGGATTGAAGGAAGGTT+GGG | 0.469467 | 4.4:-21279039 | None:intergenic |
| TTAGGCATAGAAGGGATAGT+AGG | 0.471087 | 4.4:-21278925 | None:intergenic |
| GGGCTAGGAGTTGAAGGTGG+TGG | 0.483282 | 4.4:-21279090 | None:intergenic |
| AAGAAGTAAAGCTGCAATGA+AGG | 0.485067 | 4.4:-21278746 | None:intergenic |
| TTTGGCAAAGTGGGAATGGT+AGG | 0.501400 | 4.4:-21278838 | None:intergenic |
| GGAATATTGGTAGGAAGACT+TGG | 0.503031 | 4.4:-21278967 | None:intergenic |
| AAGTGGTTGGGATTGAAGGA+AGG | 0.508086 | 4.4:-21279044 | None:intergenic |
| GGCAAAGGTGGCAATGTTGT+TGG | 0.509896 | 4.4:-21278862 | None:intergenic |
| TGCAATGAAGGATTTGGTTG+AGG | 0.510590 | 4.4:-21278734 | None:intergenic |
| AGTGAAGTGGCTGCTGGCAA+TGG | 0.520284 | 4.4:-21279015 | None:intergenic |
| GGAATTGTTGGAATATTGGT+AGG | 0.523994 | 4.4:-21278976 | None:intergenic |
| ATGTTGAGAGATGGAATTGT+TGG | 0.529497 | 4.4:-21278988 | None:intergenic |
| TTGGTAGGAAGACTTGGAAG+TGG | 0.530626 | 4.4:-21278961 | None:intergenic |
| GAAGGGATAGTAGGCAATGG+AGG | 0.560608 | 4.4:-21278916 | None:intergenic |
| GAAGTGGCTGCTGGCAATGG+TGG | 0.580562 | 4.4:-21279012 | None:intergenic |
| TTTGGAATTGAAGGCAAAGG+TGG | 0.580773 | 4.4:-21278874 | None:intergenic |
| TTGGGAAGTGAAGTGGCTGC+TGG | 0.584437 | 4.4:-21279021 | None:intergenic |
| TCAATTCCAAATATGCCTCA+AGG | 0.589431 | 4.4:+21278886 | MS.gene053569:CDS |
| GCTAGGAGTTGAAGGTGGTG+GGG | 0.597019 | 4.4:-21279088 | None:intergenic |
| GTAGGAAGACTTGGAAGTGG+TGG | 0.611131 | 4.4:-21278958 | None:intergenic |
| ATATTTGGAATTGAAGGCAA+AGG | 0.615183 | 4.4:-21278877 | None:intergenic |
| TAGTGAGTTTAGGCATAGAA+GGG | 0.616158 | 4.4:-21278933 | None:intergenic |
| ATAGAAGGGATAGTAGGCAA+TGG | 0.624968 | 4.4:-21278919 | None:intergenic |
| AGGAAGGTTGGGAAGTGAAG+TGG | 0.630070 | 4.4:-21279028 | None:intergenic |
| AATGGTGGAATGTTGAGAGA+TGG | 0.653205 | 4.4:-21278997 | None:intergenic |
| AATGGAGGAAGATTTCCTTG+AGG | 0.717776 | 4.4:-21278901 | None:intergenic |
| TAAGGGCTAGGAGTTGAAGG+TGG | 0.721449 | 4.4:-21279093 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGAATTGTTGGAATAT+TGG | - | chr4.4:21278983-21279002 | None:intergenic | 30.0% | |
| ! | ATATTTGGAATTGAAGGCAA+AGG | - | chr4.4:21278880-21278899 | None:intergenic | 30.0% |
| AAGAAGTAAAGCTGCAATGA+AGG | - | chr4.4:21278749-21278768 | None:intergenic | 35.0% | |
| AGATTTCCTTGAGGCATATT+TGG | - | chr4.4:21278895-21278914 | None:intergenic | 35.0% | |
| ATAGTGAGTTTAGGCATAGA+AGG | - | chr4.4:21278937-21278956 | None:intergenic | 35.0% | |
| ATGTTGAGAGATGGAATTGT+TGG | - | chr4.4:21278991-21279010 | None:intergenic | 35.0% | |
| GGAATTGTTGGAATATTGGT+AGG | - | chr4.4:21278979-21278998 | None:intergenic | 35.0% | |
| TAAAGCTGCAATGAAGGATT+TGG | - | chr4.4:21278743-21278762 | None:intergenic | 35.0% | |
| TAGTGAGTTTAGGCATAGAA+GGG | - | chr4.4:21278936-21278955 | None:intergenic | 35.0% | |
| TCAATTCCAAATATGCCTCA+AGG | + | chr4.4:21278886-21278905 | MS.gene053569:CDS | 35.0% | |
| ! | TGAGGCATATTTGGAATTGA+AGG | - | chr4.4:21278886-21278905 | None:intergenic | 35.0% |
| !! | GTGTTGTTGTTTGCAATAGA+TGG | - | chr4.4:21278807-21278826 | None:intergenic | 35.0% |
| AATGGAGGAAGATTTCCTTG+AGG | - | chr4.4:21278904-21278923 | None:intergenic | 40.0% | |
| ATAGAAGGGATAGTAGGCAA+TGG | - | chr4.4:21278922-21278941 | None:intergenic | 40.0% | |
| GGAATATTGGTAGGAAGACT+TGG | - | chr4.4:21278970-21278989 | None:intergenic | 40.0% | |
| GTGGGAATGGTAGGTAAATT+TGG | - | chr4.4:21278832-21278851 | None:intergenic | 40.0% | |
| TTAGGCATAGAAGGGATAGT+AGG | - | chr4.4:21278928-21278947 | None:intergenic | 40.0% | |
| ! | AATGGTGGAATGTTGAGAGA+TGG | - | chr4.4:21279000-21279019 | None:intergenic | 40.0% |
| ! | TGCAATGAAGGATTTGGTTG+AGG | - | chr4.4:21278737-21278756 | None:intergenic | 40.0% |
| ! | TTTGGAATTGAAGGCAAAGG+TGG | - | chr4.4:21278877-21278896 | None:intergenic | 40.0% |
| !! | AAGGTGGTGGGGAAAAAAAT+GGG | - | chr4.4:21279080-21279099 | None:intergenic | 40.0% |
| !!! | GTTGTTGGTTTTGGCAAAGT+GGG | - | chr4.4:21278850-21278869 | None:intergenic | 40.0% |
| !!! | TGTTGTTGGTTTTGGCAAAG+TGG | - | chr4.4:21278851-21278870 | None:intergenic | 40.0% |
| AAGTGGTTGGGATTGAAGGA+AGG | - | chr4.4:21279047-21279066 | None:intergenic | 45.0% | |
| AGTGGTGGCATAGTGAGTTT+AGG | - | chr4.4:21278946-21278965 | None:intergenic | 45.0% | |
| GGGAAAAAAATGGGAAGGAG+GGG | - | chr4.4:21279071-21279090 | None:intergenic | 45.0% | |
| GGGGAAAAAAATGGGAAGGA+GGG | - | chr4.4:21279072-21279091 | None:intergenic | 45.0% | |
| GTTGGGATTGAAGGAAGGTT+GGG | - | chr4.4:21279042-21279061 | None:intergenic | 45.0% | |
| TGGGGAAAAAAATGGGAAGG+AGG | - | chr4.4:21279073-21279092 | None:intergenic | 45.0% | |
| TGGTGGGGAAAAAAATGGGA+AGG | - | chr4.4:21279076-21279095 | None:intergenic | 45.0% | |
| TTGGTAGGAAGACTTGGAAG+TGG | - | chr4.4:21278964-21278983 | None:intergenic | 45.0% | |
| TTTGGCAAAGTGGGAATGGT+AGG | - | chr4.4:21278841-21278860 | None:intergenic | 45.0% | |
| !! | GAAGGTGGTGGGGAAAAAAA+TGG | - | chr4.4:21279081-21279100 | None:intergenic | 45.0% |
| !!! | GGTGGCAATGTTGTTGGTTT+TGG | - | chr4.4:21278859-21278878 | None:intergenic | 45.0% |
| !!! | TGGTTTTGGCAAAGTGGGAA+TGG | - | chr4.4:21278845-21278864 | None:intergenic | 45.0% |
| AAATGGGAAGGAGGGGAAAG+TGG | - | chr4.4:21279064-21279083 | None:intergenic | 50.0% | |
| AGGAAGGTTGGGAAGTGAAG+TGG | - | chr4.4:21279031-21279050 | None:intergenic | 50.0% | |
| GAAGGGATAGTAGGCAATGG+AGG | - | chr4.4:21278919-21278938 | None:intergenic | 50.0% | |
| GCAATAGATGGCGAGCTTCT+AGG | - | chr4.4:21278795-21278814 | None:intergenic | 50.0% | |
| GGCAAAGGTGGCAATGTTGT+TGG | - | chr4.4:21278865-21278884 | None:intergenic | 50.0% | |
| GGGAAAGTGGTTGGGATTGA+AGG | - | chr4.4:21279051-21279070 | None:intergenic | 50.0% | |
| GGTTGGGATTGAAGGAAGGT+TGG | - | chr4.4:21279043-21279062 | None:intergenic | 50.0% | |
| GTAGGAAGACTTGGAAGTGG+TGG | - | chr4.4:21278961-21278980 | None:intergenic | 50.0% | |
| TAAGGGCTAGGAGTTGAAGG+TGG | - | chr4.4:21279096-21279115 | None:intergenic | 50.0% | |
| AGTGAAGTGGCTGCTGGCAA+TGG | - | chr4.4:21279018-21279037 | None:intergenic | 55.0% | |
| GCTAGGAGTTGAAGGTGGTG+GGG | - | chr4.4:21279091-21279110 | None:intergenic | 55.0% | |
| GGAAGGAGGGGAAAGTGGTT+GGG | - | chr4.4:21279059-21279078 | None:intergenic | 55.0% | |
| GGCTAGGAGTTGAAGGTGGT+GGG | - | chr4.4:21279092-21279111 | None:intergenic | 55.0% | |
| TTGGGAAGTGAAGTGGCTGC+TGG | - | chr4.4:21279024-21279043 | None:intergenic | 55.0% | |
| GAAGTGGCTGCTGGCAATGG+TGG | - | chr4.4:21279015-21279034 | None:intergenic | 60.0% | |
| GGGAAGGAGGGGAAAGTGGT+TGG | - | chr4.4:21279060-21279079 | None:intergenic | 60.0% | |
| GGGCTAGGAGTTGAAGGTGG+TGG | - | chr4.4:21279093-21279112 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 21278730 | 21279116 | 21278730 | ID=MS.gene053569 |
| chr4.4 | mRNA | 21278730 | 21279116 | 21278730 | ID=MS.gene053569.t1;Parent=MS.gene053569 |
| chr4.4 | exon | 21278730 | 21279116 | 21278730 | ID=MS.gene053569.t1.exon1;Parent=MS.gene053569.t1 |
| chr4.4 | CDS | 21278730 | 21279116 | 21278730 | ID=cds.MS.gene053569.t1;Parent=MS.gene053569.t1 |
| Gene Sequence |
| Protein sequence |
