Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068185.t1 | PNX77523.1 | 61.5 | 78 | 20 | 2 | 1 | 76 | 1 | 70 | 1.90E-13 | 85.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068185.t1 | A0A2K3LG64 | 61.5 | 78 | 20 | 2 | 1 | 76 | 1 | 70 | 1.4e-13 | 85.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene068185 | 0.801145 | 1.03E-48 | -1.69E-46 |
| MS.gene054174 | MS.gene068185 | 0.816589 | 5.12E-52 | -1.69E-46 |
| MS.gene054175 | MS.gene068185 | 0.820238 | 7.62E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene068185 | 0.811616 | 6.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCGGTTTCTTTCAATTT+TGG | 0.151259 | 1.4:-36484236 | MS.gene068185:CDS |
| ACTCCATTGTTTGTCATCTA+TGG | 0.244370 | 1.4:-36484339 | MS.gene068185:CDS |
| ATGCTGCAACCTTCAATTGC+TGG | 0.375569 | 1.4:+36484164 | None:intergenic |
| ACAGATTTGATCGGCTTAAT+CGG | 0.375588 | 1.4:+36484191 | None:intergenic |
| ATCGCCTCAGTTGCGATCTC+AGG | 0.383382 | 1.4:-36484488 | MS.gene068185:CDS |
| AAGATTTCTTTGTGCTGAAA+AGG | 0.385174 | 1.4:+36484400 | None:intergenic |
| GAAGGTTGCAGCATCAAGAT+TGG | 0.441892 | 1.4:-36484155 | MS.gene068185:CDS |
| TGGTTTCGAAGTGGCGGTAA+TGG | 0.473427 | 1.4:+36484286 | None:intergenic |
| TCTGTTATACCAGCAATTGA+AGG | 0.481754 | 1.4:-36484173 | MS.gene068185:CDS |
| TTCTTCTTCCGCTGTCGCGT+CGG | 0.512166 | 1.4:-36484458 | MS.gene068185:CDS |
| AGGAGGAGATTTCTTCGATA+CGG | 0.536796 | 1.4:+36484420 | None:intergenic |
| TACAGTTCTCTCAACTGAAG+CGG | 0.543883 | 1.4:-36484252 | MS.gene068185:CDS |
| ATTTCTTTGTGCTGAAAAGG+AGG | 0.553505 | 1.4:+36484403 | None:intergenic |
| TTCTTCGATACGGCTGCAGA+AGG | 0.558066 | 1.4:+36484430 | None:intergenic |
| GCTGGTATAACAGATTTGAT+CGG | 0.561966 | 1.4:+36484182 | None:intergenic |
| TCGCCTCAGTTGCGATCTCA+GGG | 0.578803 | 1.4:-36484487 | MS.gene068185:CDS |
| CTTCCATAGATGACAAACAA+TGG | 0.594376 | 1.4:+36484336 | None:intergenic |
| TCATCTATGGAAGAAATCAA+AGG | 0.594897 | 1.4:-36484326 | MS.gene068185:CDS |
| ATCTTGCGGTGGTTTCGAAG+TGG | 0.613519 | 1.4:+36484277 | None:intergenic |
| AGGTTTCTCCGACGCGACAG+CGG | 0.631779 | 1.4:+36484450 | None:intergenic |
| GAGAGAACTGTAGAATCTTG+CGG | 0.642496 | 1.4:+36484263 | None:intergenic |
| TTGCGGTGGTTTCGAAGTGG+CGG | 0.652055 | 1.4:+36484280 | None:intergenic |
| TATCCCTGAGATCGCAACTG+AGG | 0.671775 | 1.4:+36484484 | None:intergenic |
| AGAACTGTAGAATCTTGCGG+TGG | 0.689722 | 1.4:+36484266 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAGTATGTCATTAGAAAAAA+TGG | + | chr1.4:36484276-36484295 | None:intergenic | 25.0% |
| ! | TATGTCATTAGAAAAAATGG+AGG | + | chr1.4:36484273-36484292 | None:intergenic | 25.0% |
| AAGATTTCTTTGTGCTGAAA+AGG | + | chr1.4:36484234-36484253 | None:intergenic | 30.0% | |
| TCATCTATGGAAGAAATCAA+AGG | - | chr1.4:36484305-36484324 | MS.gene068185:CDS | 30.0% | |
| ACTCCATTGTTTGTCATCTA+TGG | - | chr1.4:36484292-36484311 | MS.gene068185:CDS | 35.0% | |
| ATTTCTTTGTGCTGAAAAGG+AGG | + | chr1.4:36484231-36484250 | None:intergenic | 35.0% | |
| CTTCCATAGATGACAAACAA+TGG | + | chr1.4:36484298-36484317 | None:intergenic | 35.0% | |
| GCTGGTATAACAGATTTGAT+CGG | + | chr1.4:36484452-36484471 | None:intergenic | 35.0% | |
| TCTGTTATACCAGCAATTGA+AGG | - | chr1.4:36484458-36484477 | MS.gene068185:CDS | 35.0% | |
| ! | ACAGATTTGATCGGCTTAAT+CGG | + | chr1.4:36484443-36484462 | None:intergenic | 35.0% |
| ! | GAAGCGGTTTCTTTCAATTT+TGG | - | chr1.4:36484395-36484414 | MS.gene068185:CDS | 35.0% |
| AGGAGGAGATTTCTTCGATA+CGG | + | chr1.4:36484214-36484233 | None:intergenic | 40.0% | |
| GAGAGAACTGTAGAATCTTG+CGG | + | chr1.4:36484371-36484390 | None:intergenic | 40.0% | |
| TACAGTTCTCTCAACTGAAG+CGG | - | chr1.4:36484379-36484398 | MS.gene068185:CDS | 40.0% | |
| AGAACTGTAGAATCTTGCGG+TGG | + | chr1.4:36484368-36484387 | None:intergenic | 45.0% | |
| ATGCTGCAACCTTCAATTGC+TGG | + | chr1.4:36484470-36484489 | None:intergenic | 45.0% | |
| GAAGGTTGCAGCATCAAGAT+TGG | - | chr1.4:36484476-36484495 | MS.gene068185:CDS | 45.0% | |
| ATCTTGCGGTGGTTTCGAAG+TGG | + | chr1.4:36484357-36484376 | None:intergenic | 50.0% | |
| TATCCCTGAGATCGCAACTG+AGG | + | chr1.4:36484150-36484169 | None:intergenic | 50.0% | |
| TGGTTTCGAAGTGGCGGTAA+TGG | + | chr1.4:36484348-36484367 | None:intergenic | 50.0% | |
| TTCTTCGATACGGCTGCAGA+AGG | + | chr1.4:36484204-36484223 | None:intergenic | 50.0% | |
| ATCGCCTCAGTTGCGATCTC+AGG | - | chr1.4:36484143-36484162 | MS.gene068185:CDS | 55.0% | |
| TCGCCTCAGTTGCGATCTCA+GGG | - | chr1.4:36484144-36484163 | MS.gene068185:CDS | 55.0% | |
| TTCTTCTTCCGCTGTCGCGT+CGG | - | chr1.4:36484173-36484192 | MS.gene068185:CDS | 55.0% | |
| TTGCGGTGGTTTCGAAGTGG+CGG | + | chr1.4:36484354-36484373 | None:intergenic | 55.0% | |
| AGGTTTCTCCGACGCGACAG+CGG | + | chr1.4:36484184-36484203 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 36484135 | 36484518 | 36484135 | ID=MS.gene068185 |
| chr1.4 | mRNA | 36484135 | 36484518 | 36484135 | ID=MS.gene068185.t1;Parent=MS.gene068185 |
| chr1.4 | exon | 36484135 | 36484518 | 36484135 | ID=MS.gene068185.t1.exon1;Parent=MS.gene068185.t1 |
| chr1.4 | CDS | 36484135 | 36484518 | 36484135 | ID=cds.MS.gene068185.t1;Parent=MS.gene068185.t1 |
| Gene Sequence |
| Protein sequence |
