Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068299.t1 | XP_003604502.1 | 93 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 7.20E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068299.t1 | P81748 | 28.2 | 103 | 73 | 1 | 1 | 103 | 1 | 102 | 1.2e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068299.t1 | G7JJG3 | 93.0 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 5.2e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051560 | MS.gene068299 | -0.803485 | 3.41E-49 | -1.69E-46 |
| MS.gene056313 | MS.gene068299 | 0.829958 | 3.85E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene068299 | 0.823508 | 1.33E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene068299 | 0.821851 | 3.24E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene068299 | 0.823926 | 1.06E-53 | -1.69E-46 |
| MS.gene059045 | MS.gene068299 | 0.801022 | 1.10E-48 | -1.69E-46 |
| MS.gene059223 | MS.gene068299 | 0.80236 | 5.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068299.t1 | MTR_4g013730 | 92.982 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 8.35e-75 | 217 |
| MS.gene068299.t1 | MTR_0562s0020 | 92.982 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 8.35e-75 | 217 |
| MS.gene068299.t1 | MTR_7g070250 | 80.392 | 102 | 20 | 0 | 1 | 102 | 1 | 102 | 8.06e-58 | 174 |
| MS.gene068299.t1 | MTR_4g014040 | 51.327 | 113 | 55 | 0 | 1 | 113 | 1 | 113 | 4.18e-40 | 129 |
| MS.gene068299.t1 | MTR_4g014040 | 56.863 | 102 | 44 | 0 | 1 | 102 | 1 | 102 | 2.73e-39 | 127 |
| MS.gene068299.t1 | MTR_4g014040 | 56.863 | 102 | 44 | 0 | 1 | 102 | 1 | 102 | 6.10e-39 | 128 |
| MS.gene068299.t1 | MTR_7g059460 | 44.860 | 107 | 59 | 0 | 1 | 107 | 1 | 107 | 3.92e-30 | 104 |
| MS.gene068299.t1 | MTR_7g059460 | 44.660 | 103 | 57 | 0 | 1 | 103 | 1 | 103 | 1.55e-29 | 103 |
| MS.gene068299.t1 | MTR_4g127370 | 46.078 | 102 | 55 | 0 | 1 | 102 | 739 | 840 | 9.20e-25 | 97.8 |
| MS.gene068299.t1 | MTR_4g127360 | 41.748 | 103 | 60 | 0 | 1 | 103 | 1 | 103 | 1.30e-23 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068299.t1 | AT3G51660 | 59.223 | 103 | 42 | 0 | 1 | 103 | 1 | 103 | 1.54e-41 | 133 |
| MS.gene068299.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 4.09e-40 | 130 |
| MS.gene068299.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 4.09e-40 | 130 |
| MS.gene068299.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 7.11e-40 | 129 |
| MS.gene068299.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 1.08e-39 | 128 |
| MS.gene068299.t1 | AT5G57170 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 2.52e-26 | 94.7 |
| MS.gene068299.t1 | AT5G57170 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 5.43e-26 | 94.0 |
Find 23 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACCAACGTTAATTTAGA+TGG | 0.290910 | 4.4:-86602822 | MS.gene068299:CDS |
| CAGTACCATTGGCACAATAT+TGG | 0.301904 | 4.4:-86601325 | MS.gene068299:CDS |
| ACTGCTGTTTCCACAATCAT+AGG | 0.405897 | 4.4:-86602765 | MS.gene068299:CDS |
| TCAAAAGCATCACTACAGTT+TGG | 0.409372 | 4.4:+86601224 | None:intergenic |
| TCTCAAAGTCTTTGACATCA+TGG | 0.431478 | 4.4:-86601268 | MS.gene068299:CDS |
| GAAGAGTGACATACCTTGTC+GGG | 0.442658 | 4.4:+86602738 | None:intergenic |
| GTTCTAGCTTGATCAAAGAT+AGG | 0.446427 | 4.4:+86602786 | None:intergenic |
| GTTTGTGATGGTTCTATTGA+AGG | 0.461686 | 4.4:-86601448 | MS.gene068299:CDS |
| TGTTCTTGGTATAGAGAGGT+TGG | 0.461721 | 4.4:+86601299 | None:intergenic |
| AACAAAGAACCAGCTGCATA+TGG | 0.465591 | 4.4:-86601399 | MS.gene068299:CDS |
| TTTGTGATGGTTCTATTGAA+GGG | 0.504158 | 4.4:-86601447 | MS.gene068299:CDS |
| TAACGTTGGTAGAGATGTAA+AGG | 0.513197 | 4.4:+86602832 | None:intergenic |
| ATATGGTGAACTAGTTTCAA+TGG | 0.521191 | 4.4:-86601382 | MS.gene068299:CDS |
| AAGAATCTAATCAGTACCAT+TGG | 0.529298 | 4.4:-86601336 | MS.gene068299:CDS |
| GGATTCCAATATTGTGCCAA+TGG | 0.539625 | 4.4:+86601320 | None:intergenic |
| ATCTTGTTCTTGGTATAGAG+AGG | 0.571975 | 4.4:+86601295 | None:intergenic |
| ACTAGTTCACCATATGCAGC+TGG | 0.580662 | 4.4:+86601390 | None:intergenic |
| TTGTGATGGTTCTATTGAAG+GGG | 0.584227 | 4.4:-86601446 | MS.gene068299:CDS |
| TATGGTGAACTAGTTTCAAT+GGG | 0.596371 | 4.4:-86601381 | MS.gene068299:CDS |
| AGAAGAGTGACATACCTTGT+CGG | 0.618285 | 4.4:+86602737 | None:intergenic |
| GGTGAACTAGTTTCAATGGG+TGG | 0.635436 | 4.4:-86601378 | MS.gene068299:CDS |
| TTGGTAGAGATGTAAAGGCA+AGG | 0.650566 | 4.4:+86602837 | None:intergenic |
| AATCATAGGAAAACCCGACA+AGG | 0.692062 | 4.4:-86602751 | MS.gene068299:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATAAACTAATTTCAAT+GGG | - | chr4.4:86601853-86601872 | MS.gene068299:intron | 10.0% |
| !! | AAATAAAAAAGAATTTCACT+TGG | + | chr4.4:86602189-86602208 | None:intergenic | 15.0% |
| !! | GTTAAATAAACTAATTTCAA+TGG | - | chr4.4:86601852-86601871 | MS.gene068299:intron | 15.0% |
| !!! | ATTTATTTCTCAAAATCAAA+TGG | - | chr4.4:86601744-86601763 | MS.gene068299:intron | 15.0% |
| !!! | CACAAAATTAATATGAATTT+TGG | - | chr4.4:86601434-86601453 | MS.gene068299:CDS | 15.0% |
| !!! | ATTTTGGATTTTGTTGATTA+TGG | - | chr4.4:86601450-86601469 | MS.gene068299:CDS | 20.0% |
| !!! | TTGTTATTTTCAAACGATAT+TGG | + | chr4.4:86602662-86602681 | None:intergenic | 20.0% |
| ! | AAGTAAGTAAATAGCCAAAT+TGG | - | chr4.4:86602300-86602319 | MS.gene068299:intron | 25.0% |
| ! | ACATACATAGTGATATTTCA+AGG | - | chr4.4:86602449-86602468 | MS.gene068299:intron | 25.0% |
| ! | CCTCTAATGATAAAAATTGA+TGG | + | chr4.4:86602419-86602438 | None:intergenic | 25.0% |
| ! | GCATGGTATAAAAATTTATG+AGG | - | chr4.4:86602511-86602530 | MS.gene068299:intron | 25.0% |
| ! | TTGAGAAAAAATCTTGTTCT+TGG | + | chr4.4:86602794-86602813 | None:intergenic | 25.0% |
| !! | ACTGATTCATAATGTTTGAT+TGG | - | chr4.4:86601519-86601538 | MS.gene068299:intron | 25.0% |
| !! | CCATCAATTTTTATCATTAG+AGG | - | chr4.4:86602416-86602435 | MS.gene068299:intron | 25.0% |
| !! | TGTTTTACAAATAAAGCTTG+TGG | - | chr4.4:86601371-86601390 | MS.gene068299:CDS | 25.0% |
| !! | TTTTCCATCTAAATTAACGT+TGG | + | chr4.4:86601261-86601280 | None:intergenic | 25.0% |
| !!! | ATACCATGCAATTTAGTTTT+GGG | + | chr4.4:86602500-86602519 | None:intergenic | 25.0% |
| !!! | TATACCATGCAATTTAGTTT+TGG | + | chr4.4:86602501-86602520 | None:intergenic | 25.0% |
| AAGAATCTAATCAGTACCAT+TGG | - | chr4.4:86602740-86602759 | MS.gene068299:intron | 30.0% | |
| CAAATAAAGCTTGTGGAATT+TGG | - | chr4.4:86601378-86601397 | MS.gene068299:CDS | 30.0% | |
| GTTGTGTGAAAATCCTTTAA+GGG | - | chr4.4:86601407-86601426 | MS.gene068299:CDS | 30.0% | |
| TAACCCAAAACTAAATTGCA+TGG | - | chr4.4:86602494-86602513 | MS.gene068299:intron | 30.0% | |
| TCTACCAACGTTAATTTAGA+TGG | - | chr4.4:86601254-86601273 | MS.gene068299:CDS | 30.0% | |
| ! | AATTTTGTGTCAACCCTTAA+AGG | + | chr4.4:86601423-86601442 | None:intergenic | 30.0% |
| ! | ATATGGTGAACTAGTTTCAA+TGG | - | chr4.4:86602694-86602713 | MS.gene068299:intron | 30.0% |
| ! | GCTCTTTGATGCAATTTTTA+GGG | - | chr4.4:86601962-86601981 | MS.gene068299:intron | 30.0% |
| ! | GTAATTTTTGCAGTTTGTGA+TGG | - | chr4.4:86602616-86602635 | MS.gene068299:intron | 30.0% |
| ! | TATGGTGAACTAGTTTCAAT+GGG | - | chr4.4:86602695-86602714 | MS.gene068299:intron | 30.0% |
| ! | TGACAAGTTTTCTACAGAAA+AGG | + | chr4.4:86601674-86601693 | None:intergenic | 30.0% |
| ! | TGCTCTTTGATGCAATTTTT+AGG | - | chr4.4:86601961-86601980 | MS.gene068299:intron | 30.0% |
| ! | TTTGTGATGGTTCTATTGAA+GGG | - | chr4.4:86602629-86602648 | MS.gene068299:intron | 30.0% |
| !! | CATAATGTTTGATTGGTTTC+TGG | - | chr4.4:86601526-86601545 | MS.gene068299:intron | 30.0% |
| !! | TGATTGGTTTCTGGTATAAT+TGG | - | chr4.4:86601535-86601554 | MS.gene068299:intron | 30.0% |
| !!! | CAATTTAGTTTTGGGTTATG+TGG | + | chr4.4:86602492-86602511 | None:intergenic | 30.0% |
| AAATAAGACAGCAGACTAGT+TGG | - | chr4.4:86601900-86601919 | MS.gene068299:intron | 35.0% | |
| ACTTGGAACAAAGTACAACA+TGG | + | chr4.4:86602172-86602191 | None:intergenic | 35.0% | |
| AGAAAAGGCAGAGAGTAAAA+GGG | + | chr4.4:86601659-86601678 | None:intergenic | 35.0% | |
| ATCTTGTTCTTGGTATAGAG+AGG | + | chr4.4:86602784-86602803 | None:intergenic | 35.0% | |
| CTTGGAACAAAGTACAACAT+GGG | + | chr4.4:86602171-86602190 | None:intergenic | 35.0% | |
| GGTTGTGTGAAAATCCTTTA+AGG | - | chr4.4:86601406-86601425 | MS.gene068299:CDS | 35.0% | |
| TCTCAAAGTCTTTGACATCA+TGG | - | chr4.4:86602808-86602827 | MS.gene068299:CDS | 35.0% | |
| TCTTTGTGCGCAAAGTTAAT+AGG | + | chr4.4:86602271-86602290 | None:intergenic | 35.0% | |
| ! | AGCTTGTGGAATTTGGTTTA+AGG | - | chr4.4:86601385-86601404 | MS.gene068299:CDS | 35.0% |
| ! | GTTTGTGATGGTTCTATTGA+AGG | - | chr4.4:86602628-86602647 | MS.gene068299:intron | 35.0% |
| ! | TAACGTTGGTAGAGATGTAA+AGG | + | chr4.4:86601247-86601266 | None:intergenic | 35.0% |
| ! | TTGTGATGGTTCTATTGAAG+GGG | - | chr4.4:86602630-86602649 | MS.gene068299:intron | 35.0% |
| ! | TTTGCGCACAAAGAATTTTG+CGG | - | chr4.4:86602277-86602296 | MS.gene068299:intron | 35.0% |
| !! | GTTCTAGCTTGATCAAAGAT+AGG | + | chr4.4:86601293-86601312 | None:intergenic | 35.0% |
| !!! | CACAGTTTTGAGTACCAATT+TGG | + | chr4.4:86602317-86602336 | None:intergenic | 35.0% |
| AACAAAGAACCAGCTGCATA+TGG | - | chr4.4:86602677-86602696 | MS.gene068299:intron | 40.0% | |
| AATCATAGGAAAACCCGACA+AGG | - | chr4.4:86601325-86601344 | MS.gene068299:CDS | 40.0% | |
| AGAAGAGTGACATACCTTGT+CGG | + | chr4.4:86601342-86601361 | None:intergenic | 40.0% | |
| CAGAAAAGGCAGAGAGTAAA+AGG | + | chr4.4:86601660-86601679 | None:intergenic | 40.0% | |
| CAGTACCATTGGCACAATAT+TGG | - | chr4.4:86602751-86602770 | MS.gene068299:intron | 40.0% | |
| CCAGCACATAGAAGTTATCA+TGG | - | chr4.4:86602227-86602246 | MS.gene068299:intron | 40.0% | |
| CCATGATAACTTCTATGTGC+TGG | + | chr4.4:86602230-86602249 | None:intergenic | 40.0% | |
| GATAACTTCTATGTGCTGGA+CGG | + | chr4.4:86602226-86602245 | None:intergenic | 40.0% | |
| GGATTCCAATATTGTGCCAA+TGG | + | chr4.4:86602759-86602778 | None:intergenic | 40.0% | |
| TGTTCTTGGTATAGAGAGGT+TGG | + | chr4.4:86602780-86602799 | None:intergenic | 40.0% | |
| TTGGTAGAGATGTAAAGGCA+AGG | + | chr4.4:86601242-86601261 | None:intergenic | 40.0% | |
| ! | CGATGTGATAGTCTTAGAGT+TGG | - | chr4.4:86602561-86602580 | MS.gene068299:intron | 40.0% |
| !! | ACTGCTGTTTCCACAATCAT+AGG | - | chr4.4:86601311-86601330 | MS.gene068299:CDS | 40.0% |
| ACTAGTTCACCATATGCAGC+TGG | + | chr4.4:86602689-86602708 | None:intergenic | 45.0% | |
| GAAGAGTGACATACCTTGTC+GGG | + | chr4.4:86601341-86601360 | None:intergenic | 45.0% | |
| GAAGTGTACACTTCCCTGTT+TGG | - | chr4.4:86601604-86601623 | MS.gene068299:intron | 45.0% | |
| GCTCAGTGACAAACCAAACA+GGG | + | chr4.4:86601620-86601639 | None:intergenic | 45.0% | |
| GGGTTATGTGGCATTGTTGT+AGG | + | chr4.4:86602480-86602499 | None:intergenic | 45.0% | |
| TGCTCAGTGACAAACCAAAC+AGG | + | chr4.4:86601621-86601640 | None:intergenic | 45.0% | |
| ! | GGTGAACTAGTTTCAATGGG+TGG | - | chr4.4:86602698-86602717 | MS.gene068299:intron | 45.0% |
| ! | GTCGGGTTTTCCTATGATTG+TGG | + | chr4.4:86601324-86601343 | None:intergenic | 45.0% |
| ! | TAAGACAGCAGACTAGTTGG+AGG | - | chr4.4:86601903-86601922 | MS.gene068299:intron | 45.0% |
| ! | TTGGAGGCTTTTCTGTCTGT+AGG | - | chr4.4:86601919-86601938 | MS.gene068299:intron | 45.0% |
| !!! | TTTTTATTGTATAAATATAA+TGG | - | chr4.4:86601827-86601846 | MS.gene068299:intron | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 86601236 | 86602862 | 86601236 | ID=MS.gene068299 |
| chr4.4 | mRNA | 86601236 | 86602862 | 86601236 | ID=MS.gene068299.t1;Parent=MS.gene068299 |
| chr4.4 | exon | 86602752 | 86602862 | 86602752 | ID=MS.gene068299.t1.exon1;Parent=MS.gene068299.t1 |
| chr4.4 | CDS | 86602752 | 86602862 | 86602752 | ID=cds.MS.gene068299.t1;Parent=MS.gene068299.t1 |
| chr4.4 | exon | 86601236 | 86601469 | 86601236 | ID=MS.gene068299.t1.exon2;Parent=MS.gene068299.t1 |
| chr4.4 | CDS | 86601236 | 86601469 | 86601236 | ID=cds.MS.gene068299.t1;Parent=MS.gene068299.t1 |
| Gene Sequence |
| Protein sequence |
