AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068675

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068675.t1 MTR_2g070410 96.587 293 10 0 1 293 6 298 0.0 585
MS.gene068675.t1 MTR_2g070410 96.094 256 10 0 1 256 6 261 0.0 508
MS.gene068675.t1 MTR_8g021225 39.171 217 122 4 86 292 17 233 5.99e-47 157
MS.gene068675.t1 MTR_4g085590 39.631 217 121 5 86 292 29 245 1.60e-45 154
MS.gene068675.t1 MTR_4g094368 38.009 221 124 5 83 291 8 227 5.35e-44 150
MS.gene068675.t1 MTR_4g094815 34.109 258 129 7 54 292 11 246 2.15e-43 149
MS.gene068675.t1 MTR_4g094898 35.659 258 125 8 54 292 11 246 3.36e-42 145
MS.gene068675.t1 MTR_4g094925 37.004 227 113 9 86 292 25 241 1.36e-40 141
MS.gene068675.t1 MTR_6g091680 36.574 216 128 3 86 292 21 236 1.71e-40 141
MS.gene068675.t1 MTR_8g101900 40.278 216 120 4 86 292 18 233 3.09e-40 140
MS.gene068675.t1 MTR_4g094812 36.444 225 117 8 86 292 28 244 8.91e-40 139
MS.gene068675.t1 MTR_4g094925 37.949 195 95 7 115 292 25 210 4.93e-37 131
MS.gene068675.t1 MTR_4g094898 39.691 194 92 6 115 292 1 185 5.04e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068675.t1 AT3G62000 75.697 251 59 1 45 293 28 278 1.39e-141 400
MS.gene068675.t1 AT3G62000 74.477 239 59 1 45 281 28 266 2.34e-129 372
MS.gene068675.t1 AT3G61990 74.892 231 55 1 63 293 63 290 8.97e-125 358
MS.gene068675.t1 AT4G26220 42.009 219 112 4 86 292 15 230 3.57e-52 171
MS.gene068675.t1 AT4G34050 39.171 217 122 4 86 292 41 257 4.48e-47 159
MS.gene068675.t1 AT4G34050 39.171 217 122 4 86 292 68 284 6.87e-47 159
MS.gene068675.t1 AT1G67990 38.710 217 122 4 86 292 16 231 3.18e-45 153
MS.gene068675.t1 AT1G67980 39.512 205 115 3 95 292 28 230 4.09e-45 152
MS.gene068675.t1 AT1G24735 36.098 205 124 2 95 292 34 238 1.28e-40 141
MS.gene068675.t1 AT1G24735 35.789 190 117 1 108 292 4 193 1.51e-38 134
MS.gene068675.t1 AT1G24735 35.789 190 117 1 108 292 100 289 4.79e-38 136
MS.gene068675.t1 AT1G67980 32.051 156 99 2 142 292 9 162 4.17e-21 88.2
MS.gene068675.t1 AT4G34050 39.316 117 64 3 183 292 30 146 1.34e-20 86.3

Find 58 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTGGTTCCCGAACATTT+TGG 0.222949 2.2:-21490050 None:intergenic
GCAATGCTTGTGCAGATTCT+TGG 0.287926 2.2:+21491589 MS.gene068675:CDS
CTTAGCAACATCAAGAGATT+TGG 0.302069 2.2:-21492294 None:intergenic
GCAACTAAACGACCTGATTC+TGG 0.314053 2.2:-21492257 None:intergenic
CATTCATAATTAGAAATTCT+AGG 0.329217 2.2:-21492516 None:intergenic
TGGTTGTATTTCAGATTCTA+AGG 0.349361 2.2:+21490141 MS.gene068675:intron
GCTTGCTGGCGTTTCACATA+AGG 0.393152 2.2:+21492328 MS.gene068675:CDS
TCTTTGAGCTTTACTAGTAT+TGG 0.394056 2.2:-21489902 None:intergenic
GCATTAGTCCTTCCAGAATC+AGG 0.399144 2.2:+21492245 MS.gene068675:CDS
TTGGTGTCTTAGGTACCTAT+TGG 0.405861 2.2:+21493448 MS.gene068675:intron
GGAGGACATAATCATACAAC+CGG 0.408180 2.2:-21490029 None:intergenic
ATAATAGGTGTCTCCTGATC+AGG 0.424494 2.2:+21491555 MS.gene068675:intron
CTAGAATTTCTAATTATGAA+TGG 0.426859 2.2:+21492517 MS.gene068675:CDS
ATTATGTCCTCCAAAATGTT+CGG 0.428029 2.2:+21490040 MS.gene068675:CDS
ATTTGAATTATGCAACAACT+TGG 0.430353 2.2:+21489872 MS.gene068675:CDS
AAGAAGTATTATCAGCTTGC+TGG 0.435483 2.2:+21492314 MS.gene068675:CDS
GCCAGTAGTTGTGCCTGATC+AGG 0.436714 2.2:-21491568 None:intergenic
TTATGTCCTCCAAAATGTTC+GGG 0.440034 2.2:+21490041 MS.gene068675:CDS
TCCTGATCAGGCACAACTAC+TGG 0.443207 2.2:+21491567 MS.gene068675:CDS
ATTAACTTGGTGTTTCATCA+TGG 0.444634 2.2:+21489793 MS.gene068675:CDS
CTGTTCCAGGTGAGGATTGG+AGG 0.447610 2.2:+21493130 MS.gene068675:intron
TTTGAATTATGCAACAACTT+GGG 0.452984 2.2:+21489873 MS.gene068675:CDS
TTGTAATTGATAATGTCCTT+TGG 0.454375 2.2:+21493158 MS.gene068675:CDS
TAAGCGTGTAAGCATCAGTA+TGG 0.471614 2.2:+21493334 MS.gene068675:CDS
ATTGTCATCCCATCTCCAAT+AGG 0.475142 2.2:-21493463 None:intergenic
CTAATTATGAATGGTGAAGC+TGG 0.488384 2.2:+21492526 MS.gene068675:CDS
AGTAAAGCTCAAAGATTGAA+AGG 0.493916 2.2:+21489910 MS.gene068675:CDS
TGATGGGAGATACCTGCATC+TGG 0.495028 2.2:-21490199 None:intergenic
CATTTATTGATGCTGAGAAA+AGG 0.495349 2.2:+21492964 MS.gene068675:CDS
ATTCATAATTAGAAATTCTA+GGG 0.503168 2.2:-21492515 None:intergenic
TCTTAGGTACCTATTGGAGA+TGG 0.503427 2.2:+21493454 MS.gene068675:intron
TACTAGGTGGATGTAAAACT+TGG 0.505836 2.2:+21492478 MS.gene068675:intron
AGATTTGGCATCTCTTTCAC+AGG 0.510023 2.2:-21492279 None:intergenic
TTAACTTGGTGTTTCATCAT+GGG 0.511784 2.2:+21489794 MS.gene068675:CDS
TTGAATTATGCAACAACTTG+GGG 0.512983 2.2:+21489874 MS.gene068675:CDS
GACAAATAGTAGCATTAACT+TGG 0.515595 2.2:+21489780 MS.gene068675:CDS
GCAGAACGGTGTATTGAAGT+TGG 0.528745 2.2:+21491613 MS.gene068675:CDS
GTAAAGCTCAAAGATTGAAA+GGG 0.544679 2.2:+21489911 MS.gene068675:CDS
GTGCTGTTCCAGGTGAGGAT+TGG 0.549400 2.2:+21493127 MS.gene068675:intron
CTTAGGTACCTATTGGAGAT+GGG 0.550007 2.2:+21493455 MS.gene068675:intron
TATGCATGGAAGCCAGATGC+AGG 0.552563 2.2:+21490187 MS.gene068675:CDS
CCATTTATTTACCAACGGAT+CGG 0.554596 2.2:-21493194 None:intergenic
AATAACAATCTCATTACTGA+TGG 0.556669 2.2:-21489959 None:intergenic
CACTAGTGAGACCTTGTGCA+AGG 0.561324 2.2:+21489845 MS.gene068675:CDS
ATTGATAATGTCCTTTGGCA+TGG 0.562307 2.2:+21493163 MS.gene068675:CDS
TGGAAAAGTTGCCGATCCGT+TGG 0.582541 2.2:+21493183 MS.gene068675:CDS
TGCAGATTCTTGGAGCAGAA+CGG 0.585514 2.2:+21491599 MS.gene068675:CDS
TTATGAATGGTGAAGCTGGA+AGG 0.590746 2.2:+21492530 MS.gene068675:CDS
GCGGAGACTGCATCTATGCA+TGG 0.604771 2.2:+21490173 MS.gene068675:CDS
GATTCTAAGGCAGTTGCGAG+CGG 0.634084 2.2:+21490154 MS.gene068675:intron
AGTGAAGTTACATGTTCATG+AGG 0.637993 2.2:-21489826 None:intergenic
GAGATGGGATGACAATCTGT+CGG 0.642936 2.2:+21493470 MS.gene068675:CDS
CCAAAATGTTCGGGAACCAG+AGG 0.643528 2.2:+21490050 MS.gene068675:CDS
TTCAAATTAAACCTTGCACA+AGG 0.652684 2.2:-21489856 None:intergenic
AGGTTGTTAGTCTTACGCCC+CGG 0.659105 2.2:+21490010 MS.gene068675:CDS
CTAAACGACCTGATTCTGGA+AGG 0.659892 2.2:-21492253 None:intergenic
GAGGACATAATCATACAACC+GGG 0.716196 2.2:-21490028 None:intergenic
AGGACATAATCATACAACCG+GGG 0.774142 2.2:-21490027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATTATTATTATTTTT+TGG - chr2.2:21491192-21491211 None:intergenic 0.0%
!! AAATCTTTCTATATTATCAT+AGG - chr2.2:21491990-21492009 None:intergenic 15.0%
!! AAATTGTCTTAATCTTAATA+GGG + chr2.2:21491765-21491784 MS.gene068675:intron 15.0%
!! AAGAATTTATAAATCATGTA+TGG - chr2.2:21490394-21490413 None:intergenic 15.0%
!! ATTCATAATTAGAAATTCTA+GGG - chr2.2:21492518-21492537 None:intergenic 15.0%
!! GTATTAGTAATTAGAAAAAA+TGG - chr2.2:21493370-21493389 None:intergenic 15.0%
!! TAAATTGTCTTAATCTTAAT+AGG + chr2.2:21491764-21491783 MS.gene068675:intron 15.0%
!!! AGTTTTAATTTAATGATACT+AGG + chr2.2:21492462-21492481 MS.gene068675:intron 15.0%
!!! TAGTTTTAAATTGTGAAATT+TGG + chr2.2:21491394-21491413 MS.gene068675:intron 15.0%
!! AAATTTGCATAACAAAATGA+TGG - chr2.2:21490219-21490238 None:intergenic 20.0%
!! AATTTGCATAACAAAATGAT+GGG - chr2.2:21490218-21490237 None:intergenic 20.0%
!! CATTCATAATTAGAAATTCT+AGG - chr2.2:21492519-21492538 None:intergenic 20.0%
!! CTAATTAACATCATATAGTT+AGG - chr2.2:21492889-21492908 None:intergenic 20.0%
!! CTAGAATTTCTAATTATGAA+TGG + chr2.2:21492517-21492536 MS.gene068675:CDS 20.0%
!! GAAAATTTAACTCTTTGATT+AGG - chr2.2:21490766-21490785 None:intergenic 20.0%
!! GTTTACTCTTTAGATTAAAA+TGG - chr2.2:21491966-21491985 None:intergenic 20.0%
!! TGATACATTGTCTTTATAAT+AGG + chr2.2:21491540-21491559 MS.gene068675:intron 20.0%
!!! AAATTTTAATCAACGGTTAA+TGG + chr2.2:21493307-21493326 MS.gene068675:CDS 20.0%
!!! ATTATATTGTTAATTTGACC+AGG + chr2.2:21492202-21492221 MS.gene068675:intron 20.0%
!!! ATTCAAAACTACTAGTTTAT+TGG - chr2.2:21490447-21490466 None:intergenic 20.0%
!!! GCATTAGAAATTTTAATCAA+CGG + chr2.2:21493300-21493319 MS.gene068675:CDS 20.0%
!!! GTTATTTCTATATCAAAAGA+CGG - chr2.2:21492740-21492759 None:intergenic 20.0%
!!! TTATATTGTTAATTTGACCA+GGG + chr2.2:21492203-21492222 MS.gene068675:intron 20.0%
!!! TTGATATGTTCTCTTTTTTA+GGG + chr2.2:21492846-21492865 MS.gene068675:intron 20.0%
!!! TTTAATTTAATGATACTAGG+TGG + chr2.2:21492465-21492484 MS.gene068675:intron 20.0%
!!! TTTGATATGTTCTCTTTTTT+AGG + chr2.2:21492845-21492864 MS.gene068675:intron 20.0%
!!! TTTGATTTTAGGATTTAAGT+CGG + chr2.2:21491349-21491368 MS.gene068675:intron 20.0%
!!! TTTTTTAAACTACCAAATGT+AGG - chr2.2:21490839-21490858 None:intergenic 20.0%
! AACAATGATGTGCATATTAT+AGG + chr2.2:21492116-21492135 MS.gene068675:intron 25.0%
! AATAACAATCTCATTACTGA+TGG - chr2.2:21489962-21489981 None:intergenic 25.0%
! AATAAGCTAAAAACAGCTTA+TGG - chr2.2:21492649-21492668 None:intergenic 25.0%
! ATAACAAAAGTAACTCCAAA+GGG - chr2.2:21491936-21491955 None:intergenic 25.0%
! ATCCATCTCAAATATAAGAT+AGG - chr2.2:21490637-21490656 None:intergenic 25.0%
! ATCCTATCTTATATTTGAGA+TGG + chr2.2:21490632-21490651 MS.gene068675:intron 25.0%
! ATTAAAACTTCAGAGAATTC+AGG - chr2.2:21492451-21492470 None:intergenic 25.0%
! ATTAAGTAGTTTATCCAAAC+AGG + chr2.2:21492776-21492795 MS.gene068675:intron 25.0%
! ATTAGATCTTGTTCTACATA+TGG + chr2.2:21491713-21491732 MS.gene068675:intron 25.0%
! ATTGCTAGTGATGAAAAATA+TGG + chr2.2:21489979-21489998 MS.gene068675:CDS 25.0%
! ATTTGAATTATGCAACAACT+TGG + chr2.2:21489872-21489891 MS.gene068675:CDS 25.0%
! TATAACAAAAGTAACTCCAA+AGG - chr2.2:21491937-21491956 None:intergenic 25.0%
! TATGTAGAACAAGATCTAAT+TGG - chr2.2:21491713-21491732 None:intergenic 25.0%
! TGAGTTTCATCCAAAAATTA+AGG - chr2.2:21491890-21491909 None:intergenic 25.0%
! TTTGAATTATGCAACAACTT+GGG + chr2.2:21489873-21489892 MS.gene068675:CDS 25.0%
!! AGCACAATCATTTATTTCTT+CGG - chr2.2:21491420-21491439 None:intergenic 25.0%
!! AGGTTTGTTCTAATGTTTAT+GGG - chr2.2:21490819-21490838 None:intergenic 25.0%
!! ATATGATATATCGTCTCTTT+TGG + chr2.2:21493429-21493448 MS.gene068675:intron 25.0%
!! CAACATTTTTACTTCATAGA+AGG - chr2.2:21491519-21491538 None:intergenic 25.0%
!! GTCTCTATAACCTTAATTTT+TGG + chr2.2:21491877-21491896 MS.gene068675:intron 25.0%
!! TAGGTTTGTTCTAATGTTTA+TGG - chr2.2:21490820-21490839 None:intergenic 25.0%
!! TTGGTAGTTTAAAAAACAAG+AGG + chr2.2:21490843-21490862 MS.gene068675:intron 25.0%
!!! CTTATTTTGATAAGCTATCT+AGG + chr2.2:21492663-21492682 MS.gene068675:intron 25.0%
!!! GCTAATTACCTTATTAGTTT+TGG + chr2.2:21490984-21491003 MS.gene068675:intron 25.0%
!!! TCCTTTATTTTTCACAGTAT+AGG + chr2.2:21491651-21491670 MS.gene068675:intron 25.0%
!!! TTGATTTTAGGATTTAAGTC+GGG + chr2.2:21491350-21491369 MS.gene068675:intron 25.0%
!!! TTGTAATTGATAATGTCCTT+TGG + chr2.2:21493158-21493177 MS.gene068675:CDS 25.0%
AAAATCGCAATCAAAATCGA+AGG + chr2.2:21491095-21491114 MS.gene068675:intron 30.0%
AAAATCGCAATCAAAATCGA+TGG + chr2.2:21491095-21491114 MS.gene068675:intron 30.0%
AATACACTTCCAATACACTT+AGG + chr2.2:21493385-21493404 MS.gene068675:intron 30.0%
AATATGTATGTGATACCCTT+TGG + chr2.2:21491918-21491937 MS.gene068675:intron 30.0%
ACTTGAACAAGATATGAGAA+TGG + chr2.2:21492611-21492630 MS.gene068675:intron 30.0%
AGTAAAGCTCAAAGATTGAA+AGG + chr2.2:21489910-21489929 MS.gene068675:CDS 30.0%
ATACACTTCCAATACACTTA+GGG + chr2.2:21493386-21493405 MS.gene068675:intron 30.0%
ATAGTTAATAAATGCCGTGT+GGG - chr2.2:21491831-21491850 None:intergenic 30.0%
ATTATGTCCTCCAAAATGTT+CGG + chr2.2:21490040-21490059 MS.gene068675:CDS 30.0%
CATTAGAACAAACCTACATT+TGG + chr2.2:21490824-21490843 MS.gene068675:intron 30.0%
CATTTATTGATGCTGAGAAA+AGG + chr2.2:21492964-21492983 MS.gene068675:CDS 30.0%
GAAAGTAGTGGTATACAAAT+GGG - chr2.2:21490094-21490113 None:intergenic 30.0%
GAAATCATAGCTGCATAAAA+AGG - chr2.2:21492943-21492962 None:intergenic 30.0%
GACAAATAGTAGCATTAACT+TGG + chr2.2:21489780-21489799 MS.gene068675:CDS 30.0%
GATGCCAAGTTTAAAAATCT+TGG - chr2.2:21492160-21492179 None:intergenic 30.0%
GCATTATAAAATAGCCAACA+TGG + chr2.2:21493029-21493048 MS.gene068675:intron 30.0%
GCCTATACTGTGAAAAATAA+AGG - chr2.2:21491655-21491674 None:intergenic 30.0%
GTAAAGCTCAAAGATTGAAA+GGG + chr2.2:21489911-21489930 MS.gene068675:CDS 30.0%
GTAATTAGCAATCAAATACG+CGG - chr2.2:21490973-21490992 None:intergenic 30.0%
GTACTGCAAAAAATGTATCT+TGG - chr2.2:21490946-21490965 None:intergenic 30.0%
GTTATGCAAATTTATTCGCA+CGG + chr2.2:21490226-21490245 MS.gene068675:intron 30.0%
TAATTAGCAATCAAATACGC+GGG - chr2.2:21490972-21490991 None:intergenic 30.0%
TATATTTCCAAGTGAAAGTG+TGG - chr2.2:21492399-21492418 None:intergenic 30.0%
TATCACATATGCTCAACAAA+AGG - chr2.2:21490116-21490135 None:intergenic 30.0%
TATTACAGATTGAACCATGT+TGG - chr2.2:21493046-21493065 None:intergenic 30.0%
TCAAATAAGCTAGTTCAAAC+AGG - chr2.2:21492583-21492602 None:intergenic 30.0%
TCTGTATATTCATAGTGACA+AGG - chr2.2:21490498-21490517 None:intergenic 30.0%
TCTTTCTTTAGTAACTGATG+AGG + chr2.2:21492178-21492197 MS.gene068675:intron 30.0%
TCTTTGAGCTTTACTAGTAT+TGG - chr2.2:21489905-21489924 None:intergenic 30.0%
TCTTTGCAGATTATGATGAA+CGG + chr2.2:21492037-21492056 MS.gene068675:intron 30.0%
TGAAAAATATGGCAATAAGC+AGG + chr2.2:21489990-21490009 MS.gene068675:CDS 30.0%
TGAAGCCATTTATTTACCAA+CGG - chr2.2:21493202-21493221 None:intergenic 30.0%
TGGTTGTATTTCAGATTCTA+AGG + chr2.2:21490141-21490160 MS.gene068675:intron 30.0%
TGTACATGCATCAAACAATT+TGG - chr2.2:21490264-21490283 None:intergenic 30.0%
TTCAAATTAAACCTTGCACA+AGG - chr2.2:21489859-21489878 None:intergenic 30.0%
TTGAATTATGCAACAACTTG+GGG + chr2.2:21489874-21489893 MS.gene068675:CDS 30.0%
! AAATCGAAGGATTTTACTCA+TGG + chr2.2:21491108-21491127 MS.gene068675:intron 30.0%
! ATTGAAGTTTTCACCTTTCA+AGG - chr2.2:21490528-21490547 None:intergenic 30.0%
! CATGCCAAGATTTTTAAACT+TGG + chr2.2:21492153-21492172 MS.gene068675:intron 30.0%
! CTTATGCTTTTGATCTCATA+AGG + chr2.2:21492355-21492374 MS.gene068675:intron 30.0%
! GAACTGGTATAACAACTTTT+GGG - chr2.2:21493097-21493116 None:intergenic 30.0%
! GACTTTTGTAGAATCGATAT+AGG + chr2.2:21491130-21491149 MS.gene068675:intron 30.0%
! GGTTTGTTCTAATGTTTATG+GGG - chr2.2:21490818-21490837 None:intergenic 30.0%
! TGAACTGGTATAACAACTTT+TGG - chr2.2:21493098-21493117 None:intergenic 30.0%
! TTGAAGTTTTCACCTTTCAA+GGG - chr2.2:21490527-21490546 None:intergenic 30.0%
!! ATTAACTTGGTGTTTCATCA+TGG + chr2.2:21489793-21489812 MS.gene068675:CDS 30.0%
!! GTCGCAAAATCTTTGATTTT+AGG + chr2.2:21491338-21491357 MS.gene068675:intron 30.0%
!! TTAACTTGGTGTTTCATCAT+GGG + chr2.2:21489794-21489813 MS.gene068675:CDS 30.0%
!! TTATTTTTCACAGTATAGGC+AGG + chr2.2:21491655-21491674 MS.gene068675:intron 30.0%
!!! AACTAGTAGTTTTGAATCAG+TGG + chr2.2:21490450-21490469 MS.gene068675:intron 30.0%
AAACTCGCAATCAAAATGGA+TGG + chr2.2:21491160-21491179 MS.gene068675:intron 35.0%
AAGGATGAATGGATAAGAGT+AGG + chr2.2:21490583-21490602 MS.gene068675:intron 35.0%
ACATGGTACATTAGAAGTCA+AGG - chr2.2:21493254-21493273 None:intergenic 35.0%
AGAAGTCAAGGTCATTTAAC+TGG - chr2.2:21493242-21493261 None:intergenic 35.0%
AGTGAAGTTACATGTTCATG+AGG - chr2.2:21489829-21489848 None:intergenic 35.0%
ATGCTATGGTTGTCAAAATC+GGG + chr2.2:21491029-21491048 MS.gene068675:intron 35.0%
CATAGTTAATAAATGCCGTG+TGG - chr2.2:21491832-21491851 None:intergenic 35.0%
CATGATTGGATAAGGATGAA+TGG + chr2.2:21490572-21490591 MS.gene068675:intron 35.0%
CATGCTATGGTTGTCAAAAT+CGG + chr2.2:21491028-21491047 MS.gene068675:intron 35.0%
CCATTTATTTACCAACGGAT+CGG - chr2.2:21493197-21493216 None:intergenic 35.0%
CCTCATTAAACTGTAGTGTT+GGG + chr2.2:21492810-21492829 MS.gene068675:intron 35.0%
CTAATTATGAATGGTGAAGC+TGG + chr2.2:21492526-21492545 MS.gene068675:CDS 35.0%
CTATGGATTTAAGTCGTTGA+AGG + chr2.2:21491307-21491326 MS.gene068675:intron 35.0%
CTGTAAACTCGCAATCAAAA+TGG + chr2.2:21491156-21491175 MS.gene068675:intron 35.0%
CTTAGCAACATCAAGAGATT+TGG - chr2.2:21492297-21492316 None:intergenic 35.0%
GACAGACACCAAAACTAATA+AGG - chr2.2:21490995-21491014 None:intergenic 35.0%
GGAAAGTAGTGGTATACAAA+TGG - chr2.2:21490095-21490114 None:intergenic 35.0%
TACAGAAAATGTCCCTTGAA+AGG + chr2.2:21490512-21490531 MS.gene068675:intron 35.0%
TACTAGGTGGATGTAAAACT+TGG + chr2.2:21492478-21492497 MS.gene068675:intron 35.0%
TCAATATCTTGTCCATGCTA+TGG + chr2.2:21491015-21491034 MS.gene068675:intron 35.0%
TCCTCATTAAACTGTAGTGT+TGG + chr2.2:21492809-21492828 MS.gene068675:intron 35.0%
TCGAAAGGGAATAGCAAAAT+CGG + chr2.2:21491065-21491084 MS.gene068675:intron 35.0%
TGAGCATATGTGATATCGAT+TGG + chr2.2:21490121-21490140 MS.gene068675:intron 35.0%
TTACTTCATAGAAGGCAAGT+GGG - chr2.2:21491511-21491530 None:intergenic 35.0%
TTATGTCCTCCAAAATGTTC+GGG + chr2.2:21490041-21490060 MS.gene068675:CDS 35.0%
TTGGTAGACATGATTGGATA+AGG + chr2.2:21490564-21490583 MS.gene068675:intron 35.0%
TTTACTTCATAGAAGGCAAG+TGG - chr2.2:21491512-21491531 None:intergenic 35.0%
! ATTCTTCGAAGTAGTTGATG+TGG + chr2.2:21490408-21490427 MS.gene068675:intron 35.0%
! CTTAGTTTGGGATTCTACTA+TGG + chr2.2:21491290-21491309 MS.gene068675:intron 35.0%
! TCTGAAGAGTACACAAATGA+AGG - chr2.2:21491857-21491876 None:intergenic 35.0%
!! AAGAAGTATTATCAGCTTGC+TGG + chr2.2:21492314-21492333 MS.gene068675:CDS 35.0%
!! ATTGATAATGTCCTTTGGCA+TGG + chr2.2:21493163-21493182 MS.gene068675:CDS 35.0%
!! CGATGGATTTTGCTTAGTTT+GGG + chr2.2:21491278-21491297 MS.gene068675:intron 35.0%
!! TCGATGGATTTTGCTTAGTT+TGG + chr2.2:21491277-21491296 MS.gene068675:intron 35.0%
!!! CTGCACTTTTGGTTTTATGT+CGG + chr2.2:21490316-21490335 MS.gene068675:intron 35.0%
!!! GGAGTTGGTTTTGAACATAT+TGG + chr2.2:21490471-21490490 MS.gene068675:intron 35.0%
!!! TAGTTTTGAATCAGTGGAGT+TGG + chr2.2:21490456-21490475 MS.gene068675:intron 35.0%
!!! TTTTGAGATGCTACTACAAC+TGG + chr2.2:21493001-21493020 MS.gene068675:CDS 35.0%
ACTTCTAATGTACCATGTGC+AGG + chr2.2:21493256-21493275 MS.gene068675:intron 40.0%
AGACATTCATGTAACCCACA+CGG + chr2.2:21491814-21491833 MS.gene068675:intron 40.0%
AGATTTGGCATCTCTTTCAC+AGG - chr2.2:21492282-21492301 None:intergenic 40.0%
AGGACATAATCATACAACCG+GGG - chr2.2:21490030-21490049 None:intergenic 40.0%
ATAATAGGTGTCTCCTGATC+AGG + chr2.2:21491555-21491574 MS.gene068675:intron 40.0%
ATGTCTTCGCTATGCAAGTT+GGG + chr2.2:21490286-21490305 MS.gene068675:intron 40.0%
ATTGTCATCCCATCTCCAAT+AGG - chr2.2:21493466-21493485 None:intergenic 40.0%
CAAAATCGGGACTTTACTTC+AGG + chr2.2:21491042-21491061 MS.gene068675:intron 40.0%
CCAATCATGTCTACCAAACA+GGG - chr2.2:21490561-21490580 None:intergenic 40.0%
CCCAACACTACAGTTTAATG+AGG - chr2.2:21492813-21492832 None:intergenic 40.0%
CGAAAGGGAATAGCAAAATC+GGG + chr2.2:21491066-21491085 MS.gene068675:intron 40.0%
CTTAGGTACCTATTGGAGAT+GGG + chr2.2:21493455-21493474 MS.gene068675:intron 40.0%
GAATCTCTCCCTAAGTGTAT+TGG - chr2.2:21493397-21493416 None:intergenic 40.0%
GACTTTACTTCAGGTCGAAA+GGG + chr2.2:21491051-21491070 MS.gene068675:intron 40.0%
GAGGACATAATCATACAACC+GGG - chr2.2:21490031-21490050 None:intergenic 40.0%
GCTCAACAAAAGGAAAGTAG+TGG - chr2.2:21490106-21490125 None:intergenic 40.0%
GGAGGACATAATCATACAAC+CGG - chr2.2:21490032-21490051 None:intergenic 40.0%
TAAGCGTGTAAGCATCAGTA+TGG + chr2.2:21493334-21493353 MS.gene068675:CDS 40.0%
TATGTCTTCGCTATGCAAGT+TGG + chr2.2:21490285-21490304 MS.gene068675:intron 40.0%
TCCAATCATGTCTACCAAAC+AGG - chr2.2:21490562-21490581 None:intergenic 40.0%
TCTACCAAACAGGGACTTTA+CGG - chr2.2:21490552-21490571 None:intergenic 40.0%
TCTTAGGTACCTATTGGAGA+TGG + chr2.2:21493454-21493473 MS.gene068675:intron 40.0%
TGTAGTTCCACACTTTCACT+TGG + chr2.2:21492389-21492408 MS.gene068675:intron 40.0%
TTATGAATGGTGAAGCTGGA+AGG + chr2.2:21492530-21492549 MS.gene068675:CDS 40.0%
! CATTAAACTGTAGTGTTGGG+AGG + chr2.2:21492813-21492832 MS.gene068675:intron 40.0%
! CCGATCCGTTGGTAAATAAA+TGG + chr2.2:21493194-21493213 MS.gene068675:intron 40.0%
! TGGGGTTTTTACTGCACTTT+TGG + chr2.2:21490305-21490324 MS.gene068675:intron 40.0%
! TTGGTGTCTTAGGTACCTAT+TGG + chr2.2:21493448-21493467 MS.gene068675:intron 40.0%
!! CAACTTTTGGGAAACAGAGT+TGG - chr2.2:21493085-21493104 None:intergenic 40.0%
!! CGATTTTGACAACCATAGCA+TGG - chr2.2:21491030-21491049 None:intergenic 40.0%
!! TTTTGGGAAACAGAGTTGGA+AGG - chr2.2:21493081-21493100 None:intergenic 40.0%
!!! ATCGTCTCTTTTGGTGTCTT+AGG + chr2.2:21493438-21493457 MS.gene068675:intron 40.0%
AACAAGAGGCTTCAGTTGTC+TGG + chr2.2:21490857-21490876 MS.gene068675:intron 45.0%
AATCCCGTAAAGTCCCTGTT+TGG + chr2.2:21490545-21490564 MS.gene068675:intron 45.0%
AATGCACCACATCAGTTGTG+CGG + chr2.2:21492075-21492094 MS.gene068675:intron 45.0%
ACACTTAGGGAGAGATTCTC+AGG + chr2.2:21493399-21493418 MS.gene068675:intron 45.0%
AGGAATAGCAACAGCCTGTT+TGG - chr2.2:21492793-21492812 None:intergenic 45.0%
ATAGATCCGCACAACTGATG+TGG - chr2.2:21492084-21492103 None:intergenic 45.0%
CAGCACTTCATTCAGTGAAC+TGG - chr2.2:21493113-21493132 None:intergenic 45.0%
CCCTGTTTGGTAGACATGAT+TGG + chr2.2:21490558-21490577 MS.gene068675:intron 45.0%
CTAAACGACCTGATTCTGGA+AGG - chr2.2:21492256-21492275 None:intergenic 45.0%
CTACCAAACAGGGACTTTAC+GGG - chr2.2:21490551-21490570 None:intergenic 45.0%
GAGATGGGATGACAATCTGT+CGG + chr2.2:21493470-21493489 MS.gene068675:CDS 45.0%
GCAACTAAACGACCTGATTC+TGG - chr2.2:21492260-21492279 None:intergenic 45.0%
GCAATGCTTGTGCAGATTCT+TGG + chr2.2:21491589-21491608 MS.gene068675:CDS 45.0%
GCAGAACGGTGTATTGAAGT+TGG + chr2.2:21491613-21491632 MS.gene068675:CDS 45.0%
GCATTAGTCCTTCCAGAATC+AGG + chr2.2:21492245-21492264 MS.gene068675:CDS 45.0%
GCTTCAGTTGTCTGGTATAC+TGG + chr2.2:21490865-21490884 MS.gene068675:intron 45.0%
GGACTTTACTTCAGGTCGAA+AGG + chr2.2:21491050-21491069 MS.gene068675:intron 45.0%
TAGAGTCACTTACCTGCACA+TGG - chr2.2:21493271-21493290 None:intergenic 45.0%
TCTTCCTCAATTCTCACCTC+TGG - chr2.2:21490069-21490088 None:intergenic 45.0%
TGCAGATTCTTGGAGCAGAA+CGG + chr2.2:21491599-21491618 MS.gene068675:CDS 45.0%
TGTCTTCGCTATGCAAGTTG+GGG + chr2.2:21490287-21490306 MS.gene068675:intron 45.0%
!! ACTGAATGAAGTGCTGTTCC+AGG + chr2.2:21493117-21493136 MS.gene068675:intron 45.0%
!! AATAATAAATATGTTATAAA+TGG + chr2.2:21491201-21491220 MS.gene068675:intron 5.0%
!!! TATATAAACTATTTTTATAT+TGG + chr2.2:21492415-21492434 MS.gene068675:intron 5.0%
CACTAGTGAGACCTTGTGCA+AGG + chr2.2:21489845-21489864 MS.gene068675:CDS 50.0%
CCAAAATGTTCGGGAACCAG+AGG + chr2.2:21490050-21490069 MS.gene068675:CDS 50.0%
GGAACCAGAGGTGAGAATTG+AGG + chr2.2:21490062-21490081 MS.gene068675:intron 50.0%
TAAGACCTCCAATCCTCACC+TGG - chr2.2:21493138-21493157 None:intergenic 50.0%
TATGCATGGAAGCCAGATGC+AGG + chr2.2:21490187-21490206 MS.gene068675:CDS 50.0%
TCCTGATCAGGCACAACTAC+TGG + chr2.2:21491567-21491586 MS.gene068675:CDS 50.0%
TGATGGGAGATACCTGCATC+TGG - chr2.2:21490202-21490221 None:intergenic 50.0%
TGGAAAAGTTGCCGATCCGT+TGG + chr2.2:21493183-21493202 MS.gene068675:CDS 50.0%
! CGGCAACTTTTCCATGCCAA+AGG - chr2.2:21493177-21493196 None:intergenic 50.0%
! CTGGTTCCCGAACATTTTGG+AGG - chr2.2:21490050-21490069 None:intergenic 50.0%
! GCTTGCTGGCGTTTCACATA+AGG + chr2.2:21492328-21492347 MS.gene068675:CDS 50.0%
!! AGGTTGTTAGTCTTACGCCC+CGG + chr2.2:21490010-21490029 MS.gene068675:CDS 50.0%
!! ATGAAGTGCTGTTCCAGGTG+AGG + chr2.2:21493122-21493141 MS.gene068675:intron 50.0%
!! CCTCTGGTTCCCGAACATTT+TGG - chr2.2:21490053-21490072 None:intergenic 50.0%
!! GATTCTAAGGCAGTTGCGAG+CGG + chr2.2:21490154-21490173 MS.gene068675:intron 50.0%
CTGTTCCAGGTGAGGATTGG+AGG + chr2.2:21493130-21493149 MS.gene068675:intron 55.0%
GCCAGTAGTTGTGCCTGATC+AGG - chr2.2:21491571-21491590 None:intergenic 55.0%
GCGGAGACTGCATCTATGCA+TGG + chr2.2:21490173-21490192 MS.gene068675:CDS 55.0%
TCGCTAGTGATGAGTAGCCC+TGG - chr2.2:21492223-21492242 None:intergenic 55.0%
! GTGCTGTTCCAGGTGAGGAT+TGG + chr2.2:21493127-21493146 MS.gene068675:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 21489778 21493501 21489778 ID=MS.gene068675
chr2.2 mRNA 21489778 21493501 21489778 ID=MS.gene068675.t1;Parent=MS.gene068675
chr2.2 exon 21489778 21490071 21489778 ID=MS.gene068675.t1.exon1;Parent=MS.gene068675.t1
chr2.2 CDS 21489778 21490071 21489778 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21490155 21490208 21490155 ID=MS.gene068675.t1.exon2;Parent=MS.gene068675.t1
chr2.2 CDS 21490155 21490208 21490155 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21491562 21491645 21491562 ID=MS.gene068675.t1.exon3;Parent=MS.gene068675.t1
chr2.2 CDS 21491562 21491645 21491562 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21492224 21492349 21492224 ID=MS.gene068675.t1.exon4;Parent=MS.gene068675.t1
chr2.2 CDS 21492224 21492349 21492224 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21492484 21492551 21492484 ID=MS.gene068675.t1.exon5;Parent=MS.gene068675.t1
chr2.2 CDS 21492484 21492551 21492484 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21492953 21493022 21492953 ID=MS.gene068675.t1.exon6;Parent=MS.gene068675.t1
chr2.2 CDS 21492953 21493022 21492953 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21493139 21493204 21493139 ID=MS.gene068675.t1.exon7;Parent=MS.gene068675.t1
chr2.2 CDS 21493139 21493204 21493139 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21493278 21493355 21493278 ID=MS.gene068675.t1.exon8;Parent=MS.gene068675.t1
chr2.2 CDS 21493278 21493355 21493278 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
chr2.2 exon 21493460 21493501 21493460 ID=MS.gene068675.t1.exon9;Parent=MS.gene068675.t1
chr2.2 CDS 21493460 21493501 21493460 ID=cds.MS.gene068675.t1;Parent=MS.gene068675.t1
Gene Sequence

>MS.gene068675

ATGACAAATAGTAGCATTAACTTGGTGTTTCATCATGGGTCAACATTGCCTCATGAACATGTAACTTCACTAGTGAGACCTTGTGCAAGGTTTAATTTGAATTATGCAACAACTTGGGGTGTTACCAATACTAGTAAAGCTCAAAGATTGAAAGGGTATAATCAGCTTGTAAGAAACAGTTCCATCAGTAATGAGATTGTTATTGCTAGTGATGAAAAATATGGCAATAAGCAGGTTGTTAGTCTTACGCCCCGGTTGTATGATTATGTCCTCCAAAATGTTCGGGAACCAGAGGTGAGAATTGAGGAAGATTCCCATTTGTATACCACTACTTTCCTTTTGTTGAGCATATGTGATATCGATTGGTTGTATTTCAGATTCTAAGGCAGTTGCGAGCGGAGACTGCATCTATGCATGGAAGCCAGATGCAGGTATCTCCCATCATTTTGTTATGCAAATTTATTCGCACGGTTCACTCATTAGCCAAATTGTTTGATGCATGTACATTATGTCTTCGCTATGCAAGTTGGGGTTTTTACTGCACTTTTGGTTTTATGTCGGTAGAGAAGCATAATTATGCGTTAAATTCATTATTTGTTTGTTTCTATTGATGCCATACATGATTTATAAATTCTTCGAAGTAGTTGATGTGGAAATCTACACTGACCAATAAACTAGTAGTTTTGAATCAGTGGAGTTGGTTTTGAACATATTGGTCCTTGTCACTATGAATATACAGAAAATGTCCCTTGAAAGGTGAAAACTTCAATCCCGTAAAGTCCCTGTTTGGTAGACATGATTGGATAAGGATGAATGGATAAGAGTAGGATAGAATATAGACATGATAATCATTTATCCTATCTTATATTTGAGATGGATAAAATAATAGCATGATATTGATATGATGTAAAATGAGAAATTACGTTTGTATTTTATTAAAAAAAATTGTTTTAATAATTTTAATATGAATTTGAATTTTTAGATGCCTAATCAAAGAGTTAAATTTTCTATGCATAATTTATCTGTATTTCTTTTAACCCCATAAACATTAGAACAAACCTACATTTGGTAGTTTAAAAAACAAGAGGCTTCAGTTGTCTGGTATACTGGTATTTGATTGCTAATTAGTACGTTATTAGATTTCGTGTCTGTCTTAATATCTTGTCCAAGATACATTTTTTGCAGTACTGACCCGCGTATTTGATTGCTAATTACCTTATTAGTTTTGGTGTCTGTCTCAATATCTTGTCCATGCTATGGTTGTCAAAATCGGGACTTTACTTCAGGTCGAAAGGGAATAGCAAAATCGGGACTCGTAAAATCGCAATCAAAATCGAAGGATTTTACTCATGGACTTTTGTAGAATCGATATAGGAAACTGTAAACTCGCAATCAAAATGGATGGATTTTACCAAAAAATAATAATAATAAATATGTTATAAATGGTATGTAGTCATAAATGACTTAGTTTATAAGAAATTGTAAAATCGCAATCAAAATCGATGGATTTTGCTTAGTTTGGGATTCTACTATGGATTTAAGTCGTTGAAGGTGAAGAAAGTCGCAAAATCTTTGATTTTAGGATTTAAGTCGGGATTTTGACAACAACGTACGTTTAGTTTTAAATTGTGAAATTTGGCCGAAGAAATAAATGATTGTGCTAGAAATAATGCATATCAGTCTCACAGTCTCGTCTGTCAAACAAAAAAAAAAAAGATTTGCATTGTAATCCCACTTGCCTTCTATGAAGTAAAAATGTTGATGATACATTGTCTTTATAATAGGTGTCTCCTGATCAGGCACAACTACTGGCAATGCTTGTGCAGATTCTTGGAGCAGAACGGTGTATTGAAGTTGGCGTTTATACTGTATGTCCTTTATTTTTCACAGTATAGGCAGGCATAGCTTCTTCATGTTATATAGTAGATGTTACCAATTAGATCTTGTTCTACATATGGTTGACATATCAGCTGATTATTTGTTATATAAATTGTCTTAATCTTAATAGGGAAAAAAAACACAGTTTGCTTTGATCTAGACATTCATGTAACCCACACGGCATTTATTAACTATGAACCTTCATTTGTGTACTCTTCAGAGTCTCTATAACCTTAATTTTTGGATGAAACTCAAATATTTTAATATGTATGTGATACCCTTTGGAGTTACTTTTGTTATAGCATTACCATTTTAATCTAAAGAGTAAACACCTATGATAATATAGAAAGATTTCAAAGTAAAAAATTGTTTAAAAACAAATCTTTGCAGATTATGATGAACGGAAAATTAAATAATCTAATGCACCACATCAGTTGTGCGGATCTATTTAATTTTTGTGAACAATGATGTGCATATTATAGGTTTCTTTCAATTGTCATGCCAAGATTTTTAAACTTGGCATCTTTCTTTAGTAACTGATGAGGTATTATATTGTTAATTTGACCAGGGCTACTCATCACTAGCGATAGCATTAGTCCTTCCAGAATCAGGTCGTTTAGTTGCCTGTGAAAGAGATGCCAAATCTCTTGATGTTGCTAAGAAGTATTATCAGCTTGCTGGCGTTTCACATAAGGTAAACTTATGCTTTTGATCTCATAAGGTGTCAAATATTTGTAGTTCCACACTTTCACTTGGAAATATATAAACTATTTTTATATTGGATTTTTATTGCCTGAATTCTCTGAAGTTTTAATTTAATGATACTAGGTGGATGTAAAACTTGGACTTGCAGCAGATTCCCTAGAATTTCTAATTATGAATGGTGAAGCTGGAAGGTACGATTCTAAACATCAAGACTCTATGCCTGTTTGAACTAGCTTATTTGAACTTAGCTACTTGAACAAGATATGAGAATGGTTTATGACATGTCCATAAGCTGTTTTTAGCTTATTTTGATAAGCTATCTAGGATAGCTTATGAAAAGAGCTTATAGTTTATATGAAAACAATTTGACTTTATTCCGTCTTTTGATATAGAAATAACTTATAAACAATGCTTAATTAAGTAGTTTATCCAAACAGGCTGTTGCTATTCCTCATTAAACTGTAGTGTTGGGAGGTCTTCTACATTTGATATGTTCTCTTTTTTAGGGTATTGTTTGCTAAGAATCCTAACTATATGATGTTAATTAGAAAAAACAAGTAAAATAGAATTTAAACATTTCCTTTTTATGCAGCTATGATTTCGCATTTATTGATGCTGAGAAAAGGATGACTGAGAAATATTTTGAGATGCTACTACAACTGGTGAGAGCATTATAAAATAGCCAACATGGTTCAATCTGTAATATTTTTCTTAAAACCTTCCAACTCTGTTTCCCAAAAGTTGTTATACCAGTTCACTGAATGAAGTGCTGTTCCAGGTGAGGATTGGAGGTCTTATTGTAATTGATAATGTCCTTTGGCATGGAAAAGTTGCCGATCCGTTGGTAAATAAATGGCTTCATATTTTTTTCGTTATGTCCAGTTAAATGACCTTGACTTCTAATGTACCATGTGCAGGTAAGTGACTCTAAAACTATCAGCATTAGAAATTTTAATCAACGGTTAATGGAAGATAAGCGTGTAAGCATCAGTATGGTAAGTTTGTACCATTTTTTCTAATTACTAATACACTTCCAATACACTTAGGGAGAGATTCTCAGGTTGATATATATGATATATCGTCTCTTTTGGTGTCTTAGGTACCTATTGGAGATGGGATGACAATCTGTCGGAAAAGATAA
Protein sequence

>MS.gene068675.t1

MTNSSINLVFHHGSTLPHEHVTSLVRPCARFNLNYATTWGVTNTSKAQRLKGYNQLVRNSSISNEIVIASDEKYGNKQVVSLTPRLYDYVLQNVREPEILRQLRAETASMHGSQMQVSPDQAQLLAMLVQILGAERCIEVGVYTGYSSLAIALVLPESGRLVACERDAKSLDVAKKYYQLAGVSHKVDVKLGLAADSLEFLIMNGEAGSYDFAFIDAEKRMTEKYFEMLLQLVRIGGLIVIDNVLWHGKVADPLVSDSKTISIRNFNQRLMEDKRVSISMVPIGDGMTICRKR