Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068805.t1 | XP_013445649.1 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 4.00E-58 | 233.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068805.t1 | O81831 | 62.3 | 114 | 42 | 1 | 3 | 115 | 11 | 124 | 5.9e-34 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068805.t1 | A0A072U1J6 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 2.9e-58 | 233.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060524 | MS.gene068805 | PPI |
| MS.gene067296 | MS.gene068805 | PPI |
| MS.gene84736 | MS.gene068805 | PPI |
| MS.gene068384 | MS.gene068805 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068805.t1 | MTR_8g466250 | 97.391 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 9.92e-79 | 227 |
| MS.gene068805.t1 | MTR_2g081580 | 77.193 | 114 | 25 | 1 | 1 | 114 | 1 | 113 | 1.16e-58 | 176 |
| MS.gene068805.t1 | MTR_1g052885 | 66.667 | 105 | 35 | 0 | 11 | 115 | 6 | 110 | 9.83e-46 | 144 |
| MS.gene068805.t1 | MTR_2g089210 | 64.762 | 105 | 37 | 0 | 11 | 115 | 10 | 114 | 8.45e-44 | 139 |
| MS.gene068805.t1 | MTR_2g011400 | 60.000 | 110 | 44 | 0 | 2 | 111 | 5 | 114 | 4.53e-40 | 129 |
| MS.gene068805.t1 | MTR_2g081300 | 68.750 | 96 | 29 | 1 | 11 | 106 | 5 | 99 | 8.27e-40 | 129 |
| MS.gene068805.t1 | MTR_8g027180 | 51.724 | 116 | 53 | 1 | 3 | 115 | 8 | 123 | 2.23e-38 | 125 |
| MS.gene068805.t1 | MTR_2g081350 | 66.667 | 96 | 31 | 1 | 11 | 106 | 9 | 103 | 4.90e-38 | 124 |
| MS.gene068805.t1 | MTR_2g081440 | 63.542 | 96 | 34 | 1 | 11 | 106 | 9 | 103 | 1.13e-35 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068805.t1 | AT4G27280 | 62.281 | 114 | 42 | 1 | 3 | 115 | 11 | 124 | 2.29e-46 | 146 |
| MS.gene068805.t1 | AT5G54490 | 57.895 | 114 | 47 | 1 | 3 | 115 | 11 | 124 | 6.59e-43 | 137 |
| MS.gene068805.t1 | AT2G46600 | 50.000 | 114 | 57 | 0 | 2 | 115 | 13 | 126 | 6.06e-38 | 125 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTTTGTTGGGATTAAA+TGG | 0.221597 | 8.4:-44559705 | MS.gene068805:CDS |
| CCATGATCACCTTCCTTTCA+TGG | 0.222260 | 8.4:-44559835 | MS.gene068805:CDS |
| TAGATTGAGTCCTGAACTTA+TGG | 0.277588 | 8.4:-44559589 | MS.gene068805:CDS |
| CCTTTCATGGCCAACAAGTT+AGG | 0.313114 | 8.4:-44559822 | MS.gene068805:CDS |
| GAAAATGAATTCAGCTTTGT+TGG | 0.357480 | 8.4:-44559715 | MS.gene068805:CDS |
| AAAATGAATTCAGCTTTGTT+GGG | 0.421549 | 8.4:-44559714 | MS.gene068805:CDS |
| GCCATCTCCACCTAACTTGT+TGG | 0.424644 | 8.4:+44559812 | None:intergenic |
| TTAGAGCCCTCCATAAGTTC+AGG | 0.443165 | 8.4:+44559579 | None:intergenic |
| CTCTAAATTGTGGTTGGAAG+AGG | 0.465900 | 8.4:-44559562 | MS.gene068805:CDS |
| AAAATCAAAATTGATGTTGA+AGG | 0.466207 | 8.4:+44559538 | None:intergenic |
| TCAAGATACAGAACTCCATT+TGG | 0.466634 | 8.4:+44559616 | None:intergenic |
| TGATAGATGAACTGTGTAAT+GGG | 0.481174 | 8.4:-44559788 | MS.gene068805:CDS |
| CCATGAAAGGAAGGTGATCA+TGG | 0.483924 | 8.4:+44559835 | None:intergenic |
| TTGATAGATGAACTGTGTAA+TGG | 0.508599 | 8.4:-44559789 | MS.gene068805:CDS |
| AGATGGTGCTCTTAACCAAA+TGG | 0.509208 | 8.4:-44559631 | MS.gene068805:CDS |
| CCTAACTTGTTGGCCATGAA+AGG | 0.509670 | 8.4:+44559822 | None:intergenic |
| GGAGGGCTCTAAATTGTGGT+TGG | 0.524013 | 8.4:-44559568 | MS.gene068805:CDS |
| GCCAACAAGTTAGGTGGAGA+TGG | 0.547465 | 8.4:-44559813 | MS.gene068805:CDS |
| AAATCAAAATTGATGTTGAA+GGG | 0.549437 | 8.4:+44559539 | None:intergenic |
| TTATGGAGGGCTCTAAATTG+TGG | 0.559343 | 8.4:-44559572 | MS.gene068805:CDS |
| ATTGAGTCCTGAACTTATGG+AGG | 0.578017 | 8.4:-44559586 | MS.gene068805:CDS |
| ACTTGTTGGCCATGAAAGGA+AGG | 0.584114 | 8.4:+44559826 | None:intergenic |
| TTCATGGCCAACAAGTTAGG+TGG | 0.592288 | 8.4:-44559819 | MS.gene068805:CDS |
| TTGAGTCCTGAACTTATGGA+GGG | 0.607807 | 8.4:-44559585 | MS.gene068805:CDS |
| CATGGAAATGAGACTGTGGT+GGG | 0.688196 | 8.4:+44559853 | None:intergenic |
| TCATGGAAATGAGACTGTGG+TGG | 0.705310 | 8.4:+44559852 | None:intergenic |
| TGATCATGGAAATGAGACTG+TGG | 0.746765 | 8.4:+44559849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATGAATTCAGCTTTGTT+GGG | - | chr8.4:44559695-44559714 | MS.gene068805:CDS | 25.0% |
| !!! | CTTTTGAAGGATTCTAATAA+AGG | - | chr8.4:44559650-44559669 | MS.gene068805:CDS | 25.0% |
| TGATAGATGAACTGTGTAAT+GGG | - | chr8.4:44559621-44559640 | MS.gene068805:CDS | 30.0% | |
| TTGATAGATGAACTGTGTAA+TGG | - | chr8.4:44559620-44559639 | MS.gene068805:CDS | 30.0% | |
| ! | ACAAAGCTGAATTCATTTTC+AGG | + | chr8.4:44559694-44559713 | None:intergenic | 30.0% |
| ! | AGATCTTTTGTCTATGCTAA+TGG | - | chr8.4:44559739-44559758 | MS.gene068805:CDS | 30.0% |
| ! | GAAAATGAATTCAGCTTTGT+TGG | - | chr8.4:44559694-44559713 | MS.gene068805:CDS | 30.0% |
| !!! | TAATGGGTTCAATCTTTTGA+AGG | - | chr8.4:44559637-44559656 | MS.gene068805:CDS | 30.0% |
| TAGATTGAGTCCTGAACTTA+TGG | - | chr8.4:44559820-44559839 | MS.gene068805:CDS | 35.0% | |
| TCAAGATACAGAACTCCATT+TGG | + | chr8.4:44559796-44559815 | None:intergenic | 35.0% | |
| ! | CTAATGGAAGGTGATTTTGA+TGG | - | chr8.4:44559755-44559774 | MS.gene068805:CDS | 35.0% |
| ! | CTTTTGTCTATGCTAATGGA+AGG | - | chr8.4:44559743-44559762 | MS.gene068805:CDS | 35.0% |
| !! | TCAGCTTTGTTGGGATTAAA+TGG | - | chr8.4:44559704-44559723 | MS.gene068805:CDS | 35.0% |
| AGATGGTGCTCTTAACCAAA+TGG | - | chr8.4:44559778-44559797 | MS.gene068805:CDS | 40.0% | |
| ATTGAGTCCTGAACTTATGG+AGG | - | chr8.4:44559823-44559842 | MS.gene068805:CDS | 40.0% | |
| CTCTAAATTGTGGTTGGAAG+AGG | - | chr8.4:44559847-44559866 | MS.gene068805:CDS | 40.0% | |
| TGATCATGGAAATGAGACTG+TGG | + | chr8.4:44559563-44559582 | None:intergenic | 40.0% | |
| TTGAGTCCTGAACTTATGGA+GGG | - | chr8.4:44559824-44559843 | MS.gene068805:CDS | 40.0% | |
| !! | GAAGGTGATTTTGATGGAGA+TGG | - | chr8.4:44559761-44559780 | MS.gene068805:CDS | 40.0% |
| !! | TTATGGAGGGCTCTAAATTG+TGG | - | chr8.4:44559837-44559856 | MS.gene068805:CDS | 40.0% |
| CATGGAAATGAGACTGTGGT+GGG | + | chr8.4:44559559-44559578 | None:intergenic | 45.0% | |
| CCATGAAAGGAAGGTGATCA+TGG | + | chr8.4:44559577-44559596 | None:intergenic | 45.0% | |
| CCATGATCACCTTCCTTTCA+TGG | - | chr8.4:44559574-44559593 | MS.gene068805:CDS | 45.0% | |
| CCTTTCATGGCCAACAAGTT+AGG | - | chr8.4:44559587-44559606 | MS.gene068805:CDS | 45.0% | |
| TCATGGAAATGAGACTGTGG+TGG | + | chr8.4:44559560-44559579 | None:intergenic | 45.0% | |
| TTAGAGCCCTCCATAAGTTC+AGG | + | chr8.4:44559833-44559852 | None:intergenic | 45.0% | |
| TTCATGGCCAACAAGTTAGG+TGG | - | chr8.4:44559590-44559609 | MS.gene068805:CDS | 45.0% | |
| ! | ACTTGTTGGCCATGAAAGGA+AGG | + | chr8.4:44559586-44559605 | None:intergenic | 45.0% |
| !! | CCTAACTTGTTGGCCATGAA+AGG | + | chr8.4:44559590-44559609 | None:intergenic | 45.0% |
| GCCAACAAGTTAGGTGGAGA+TGG | - | chr8.4:44559596-44559615 | MS.gene068805:CDS | 50.0% | |
| ! | GCCATCTCCACCTAACTTGT+TGG | + | chr8.4:44559600-44559619 | None:intergenic | 50.0% |
| !! | GGAGGGCTCTAAATTGTGGT+TGG | - | chr8.4:44559841-44559860 | MS.gene068805:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 44559542 | 44559889 | 44559542 | ID=MS.gene068805 |
| chr8.4 | mRNA | 44559542 | 44559889 | 44559542 | ID=MS.gene068805.t1;Parent=MS.gene068805 |
| chr8.4 | exon | 44559542 | 44559889 | 44559542 | ID=MS.gene068805.t1.exon1;Parent=MS.gene068805.t1 |
| chr8.4 | CDS | 44559542 | 44559889 | 44559542 | ID=cds.MS.gene068805.t1;Parent=MS.gene068805.t1 |
| Gene Sequence |
| Protein sequence |
