Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068384.t1 | XP_003627531.1 | 97.6 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 2.70E-60 | 241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068384.t1 | Q9ZPX9 | 66.9 | 124 | 41 | 0 | 2 | 125 | 5 | 128 | 6.4e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068384.t1 | G7LAU8 | 97.6 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 2.0e-60 | 241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052674 | MS.gene068384 | 0.805418 | 1.35E-49 | -1.69E-46 |
| MS.gene052675 | MS.gene068384 | 0.801929 | 7.14E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene068384 | 0.801801 | 7.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90533 | MS.gene068384 | PPI |
| MS.gene000304 | MS.gene068384 | PPI |
| MS.gene068384 | MS.gene35762 | PPI |
| MS.gene068384 | MS.gene032094 | PPI |
| MS.gene31568 | MS.gene068384 | PPI |
| MS.gene068384 | MS.gene51384 | PPI |
| MS.gene068384 | MS.gene25536 | PPI |
| MS.gene068384 | MS.gene45281 | PPI |
| MS.gene031332 | MS.gene068384 | PPI |
| MS.gene068384 | MS.gene068805 | PPI |
| MS.gene064243 | MS.gene068384 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068384.t1 | MTR_8g027180 | 97.600 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 6.83e-86 | 246 |
| MS.gene068384.t1 | MTR_2g081580 | 48.182 | 110 | 57 | 0 | 16 | 125 | 7 | 116 | 4.31e-37 | 122 |
| MS.gene068384.t1 | MTR_2g011400 | 57.282 | 103 | 44 | 0 | 17 | 119 | 12 | 114 | 1.99e-36 | 120 |
| MS.gene068384.t1 | MTR_2g089210 | 52.778 | 108 | 51 | 0 | 17 | 124 | 8 | 115 | 2.08e-36 | 120 |
| MS.gene068384.t1 | MTR_8g466250 | 49.138 | 116 | 56 | 1 | 8 | 123 | 3 | 115 | 3.25e-36 | 120 |
| MS.gene068384.t1 | MTR_1g052885 | 52.381 | 105 | 50 | 0 | 19 | 123 | 6 | 110 | 7.13e-35 | 117 |
| MS.gene068384.t1 | MTR_2g081300 | 55.789 | 95 | 41 | 1 | 15 | 109 | 1 | 94 | 9.25e-30 | 103 |
| MS.gene068384.t1 | MTR_2g081440 | 55.319 | 94 | 41 | 1 | 16 | 109 | 6 | 98 | 4.99e-29 | 102 |
| MS.gene068384.t1 | MTR_2g081350 | 51.020 | 98 | 47 | 1 | 17 | 114 | 7 | 103 | 1.09e-27 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068384.t1 | AT2G46600 | 68.908 | 119 | 37 | 0 | 7 | 125 | 10 | 128 | 1.74e-57 | 174 |
| MS.gene068384.t1 | AT4G27280 | 43.548 | 124 | 69 | 1 | 1 | 123 | 1 | 124 | 2.12e-31 | 108 |
| MS.gene068384.t1 | AT5G54490 | 40.323 | 124 | 73 | 1 | 1 | 123 | 1 | 124 | 8.41e-29 | 102 |
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAACAAGTGAGAGTCTT+AGG | 0.273916 | 8.3:+67134497 | MS.gene068384:CDS |
| TTGTTATCAAACCTGTTTCT+TGG | 0.291271 | 8.3:-67134483 | None:intergenic |
| CTGTTTCTTGGTCTGCTAGA+AGG | 0.333027 | 8.3:-67134471 | None:intergenic |
| AAATTCTACTTCCATGGTTG+TGG | 0.347527 | 8.3:-67134374 | None:intergenic |
| TCAGCATCTTCCATCATTCC+AGG | 0.368038 | 8.3:-67134652 | None:intergenic |
| TCAAGCTTCTCGGCCATGAT+TGG | 0.382367 | 8.3:-67134409 | None:intergenic |
| AATTCTACTTCCATGGTTGT+GGG | 0.400539 | 8.3:-67134373 | None:intergenic |
| CTTCTAGCAGACCAAGAAAC+AGG | 0.415817 | 8.3:+67134472 | MS.gene068384:CDS |
| TGAAGACTTGCTCCCAATCA+TGG | 0.425482 | 8.3:+67134396 | MS.gene068384:CDS |
| GGTTTCTACATCAAGCTTCT+CGG | 0.435006 | 8.3:-67134419 | None:intergenic |
| GTCTTCAAATTCTACTTCCA+TGG | 0.455751 | 8.3:-67134380 | None:intergenic |
| CAAGCTTCTCGGCCATGATT+GGG | 0.467944 | 8.3:-67134408 | None:intergenic |
| TAAAGAAGAAGCAGAAGCTA+TGG | 0.478731 | 8.3:+67134555 | MS.gene068384:CDS |
| TTCTACTTCCATGGTTGTGG+GGG | 0.482418 | 8.3:-67134371 | None:intergenic |
| CCTCTACAATGGAAACCAAC+AGG | 0.495302 | 8.3:+67134332 | None:intergenic |
| AAGGCTTAGTCCTGGAATGA+TGG | 0.496275 | 8.3:+67134642 | MS.gene068384:CDS |
| GGTTTGCTTCATGTTCCTGT+TGG | 0.497765 | 8.3:-67134347 | None:intergenic |
| AAACTCGGCCATGCTTGGTA+TGG | 0.505287 | 8.3:+67134522 | MS.gene068384:CDS |
| ATTCTACTTCCATGGTTGTG+GGG | 0.521124 | 8.3:-67134372 | None:intergenic |
| GTTAAACAAGGTGATCTTGA+TGG | 0.522373 | 8.3:+67134577 | MS.gene068384:CDS |
| TCGGCCATGCTTGGTATGGA+AGG | 0.544269 | 8.3:+67134526 | MS.gene068384:CDS |
| TTGGTTGGAGAAAGCAATTG+AGG | 0.545757 | 8.3:+67134678 | MS.gene068384:CDS |
| CAAGGTGATCTTGATGGTGA+TGG | 0.548309 | 8.3:+67134583 | MS.gene068384:CDS |
| TGATGGAAGATGCTGAAACT+TGG | 0.556410 | 8.3:+67134659 | MS.gene068384:CDS |
| GGAAGATGCTGAAACTTGGT+TGG | 0.562957 | 8.3:+67134663 | MS.gene068384:CDS |
| TTGATGGTAAGGCTTAGTCC+TGG | 0.584659 | 8.3:+67134634 | MS.gene068384:CDS |
| ACCACATAGTTCAGATACAA+AGG | 0.600386 | 8.3:-67134440 | None:intergenic |
| CATTCCTTCCATACCAAGCA+TGG | 0.613828 | 8.3:-67134530 | None:intergenic |
| TTTGTATCTGAACTATGTGG+TGG | 0.640181 | 8.3:+67134442 | MS.gene068384:CDS |
| GCAGAAGCTATGGTTAAACA+AGG | 0.656352 | 8.3:+67134565 | MS.gene068384:CDS |
| ACCTTTGTATCTGAACTATG+TGG | 0.695403 | 8.3:+67134439 | MS.gene068384:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGTTATCAAACCTGTTTCT+TGG | - | chr8.3:67134486-67134505 | None:intergenic | 30.0% |
| !! | AGTTTTGCATATTGATGGTA+AGG | + | chr8.3:67134623-67134642 | MS.gene068384:CDS | 30.0% |
| AAATTCTACTTCCATGGTTG+TGG | - | chr8.3:67134377-67134396 | None:intergenic | 35.0% | |
| AATTCTACTTCCATGGTTGT+GGG | - | chr8.3:67134376-67134395 | None:intergenic | 35.0% | |
| ACCACATAGTTCAGATACAA+AGG | - | chr8.3:67134443-67134462 | None:intergenic | 35.0% | |
| ACCTTTGTATCTGAACTATG+TGG | + | chr8.3:67134439-67134458 | MS.gene068384:CDS | 35.0% | |
| GTCTTCAAATTCTACTTCCA+TGG | - | chr8.3:67134383-67134402 | None:intergenic | 35.0% | |
| GTTAAACAAGGTGATCTTGA+TGG | + | chr8.3:67134577-67134596 | MS.gene068384:CDS | 35.0% | |
| TAAAGAAGAAGCAGAAGCTA+TGG | + | chr8.3:67134555-67134574 | MS.gene068384:CDS | 35.0% | |
| TGAGAGTCTTAGGAAAAACT+CGG | + | chr8.3:67134507-67134526 | MS.gene068384:CDS | 35.0% | |
| TGATAACAAGTGAGAGTCTT+AGG | + | chr8.3:67134497-67134516 | MS.gene068384:CDS | 35.0% | |
| TTTGTATCTGAACTATGTGG+TGG | + | chr8.3:67134442-67134461 | MS.gene068384:CDS | 35.0% | |
| ! | GACTGAGTTTTGCATATTGA+TGG | + | chr8.3:67134618-67134637 | MS.gene068384:CDS | 35.0% |
| ATTCTACTTCCATGGTTGTG+GGG | - | chr8.3:67134375-67134394 | None:intergenic | 40.0% | |
| GCAGAAGCTATGGTTAAACA+AGG | + | chr8.3:67134565-67134584 | MS.gene068384:CDS | 40.0% | |
| GGTTTCTACATCAAGCTTCT+CGG | - | chr8.3:67134422-67134441 | None:intergenic | 40.0% | |
| TGATGGAAGATGCTGAAACT+TGG | + | chr8.3:67134659-67134678 | MS.gene068384:CDS | 40.0% | |
| TTGGTTGGAGAAAGCAATTG+AGG | + | chr8.3:67134678-67134697 | MS.gene068384:CDS | 40.0% | |
| AAGGCTTAGTCCTGGAATGA+TGG | + | chr8.3:67134642-67134661 | MS.gene068384:CDS | 45.0% | |
| CATTCCTTCCATACCAAGCA+TGG | - | chr8.3:67134533-67134552 | None:intergenic | 45.0% | |
| CTTCTAGCAGACCAAGAAAC+AGG | + | chr8.3:67134472-67134491 | MS.gene068384:CDS | 45.0% | |
| GGAAGATGCTGAAACTTGGT+TGG | + | chr8.3:67134663-67134682 | MS.gene068384:CDS | 45.0% | |
| TCAGCATCTTCCATCATTCC+AGG | - | chr8.3:67134655-67134674 | None:intergenic | 45.0% | |
| TTCTACTTCCATGGTTGTGG+GGG | - | chr8.3:67134374-67134393 | None:intergenic | 45.0% | |
| ! | CTGTTTCTTGGTCTGCTAGA+AGG | - | chr8.3:67134474-67134493 | None:intergenic | 45.0% |
| ! | GGTTTGCTTCATGTTCCTGT+TGG | - | chr8.3:67134350-67134369 | None:intergenic | 45.0% |
| ! | TGAAGACTTGCTCCCAATCA+TGG | + | chr8.3:67134396-67134415 | MS.gene068384:CDS | 45.0% |
| ! | TTGATGGTAAGGCTTAGTCC+TGG | + | chr8.3:67134634-67134653 | MS.gene068384:CDS | 45.0% |
| !! | CAAGGTGATCTTGATGGTGA+TGG | + | chr8.3:67134583-67134602 | MS.gene068384:CDS | 45.0% |
| AAACTCGGCCATGCTTGGTA+TGG | + | chr8.3:67134522-67134541 | MS.gene068384:CDS | 50.0% | |
| AGGAAAAACTCGGCCATGCT+TGG | + | chr8.3:67134517-67134536 | MS.gene068384:CDS | 50.0% | |
| CAAGCTTCTCGGCCATGATT+GGG | - | chr8.3:67134411-67134430 | None:intergenic | 50.0% | |
| TCAAGCTTCTCGGCCATGAT+TGG | - | chr8.3:67134412-67134431 | None:intergenic | 50.0% | |
| TACTTCCATGGTTGTGGGGG+TGG | - | chr8.3:67134371-67134390 | None:intergenic | 55.0% | |
| TCGGCCATGCTTGGTATGGA+AGG | + | chr8.3:67134526-67134545 | MS.gene068384:CDS | 55.0% | |
| GCAAACCACCCCCACAACCA+TGG | + | chr8.3:67134363-67134382 | MS.gene068384:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67134340 | 67134717 | 67134340 | ID=MS.gene068384 |
| chr8.3 | mRNA | 67134340 | 67134717 | 67134340 | ID=MS.gene068384.t1;Parent=MS.gene068384 |
| chr8.3 | exon | 67134340 | 67134717 | 67134340 | ID=MS.gene068384.t1.exon1;Parent=MS.gene068384.t1 |
| chr8.3 | CDS | 67134340 | 67134717 | 67134340 | ID=cds.MS.gene068384.t1;Parent=MS.gene068384.t1 |
| Gene Sequence |
| Protein sequence |
