AlfalfaGEDB | MS.gene067296

M. sativa cultivar XinJiangDaYe / MS.gene067296

KEGG: K24345
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13833.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067296.t1	XP_003627531.1	99.2	125	1	0	1	125	1	125	4.20E-61	243.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067296.t1	Q9ZPX9	66.9	124	41	0	2	125	5	128	6.4e-42	171.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067296.t1	G7LAU8	99.2	125	1	0	1	125	1	125	3.0e-61	243.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene067296	MS.gene064243	PPI
MS.gene067296	MS.gene51384	PPI
MS.gene031332	MS.gene067296	PPI
MS.gene067296	MS.gene25536	PPI
MS.gene067296	MS.gene45281	PPI
MS.gene000304	MS.gene067296	PPI
MS.gene067296	MS.gene068805	PPI
MS.gene31568	MS.gene067296	PPI
MS.gene90533	MS.gene067296	PPI
MS.gene067296	MS.gene35762	PPI
MS.gene067296	MS.gene032094	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067296.t1	MTR_8g027180	99.200	125	1	0	1	125	1	125	7.36e-87	248
MS.gene067296.t1	MTR_8g466250	49.138	116	56	1	8	123	3	115	1.51e-36	121
MS.gene067296.t1	MTR_2g081580	47.273	110	58	0	16	125	7	116	1.62e-36	121
MS.gene067296.t1	MTR_2g011400	57.426	101	43	0	19	119	14	114	3.87e-36	120
MS.gene067296.t1	MTR_2g089210	53.271	107	50	0	18	124	9	115	5.20e-36	119
MS.gene067296.t1	MTR_1g052885	52.381	105	50	0	19	123	6	110	5.43e-35	117
MS.gene067296.t1	MTR_2g081300	55.789	95	41	1	15	109	1	94	1.43e-29	103
MS.gene067296.t1	MTR_2g081440	55.319	94	41	1	16	109	6	98	8.89e-29	101
MS.gene067296.t1	MTR_2g081350	51.546	97	46	1	18	114	8	103	1.30e-27	98.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067296.t1	AT2G46600	68.908	119	37	0	7	125	10	128	1.76e-57	174
MS.gene067296.t1	AT4G27280	44.355	124	68	1	1	123	1	124	3.74e-32	110
MS.gene067296.t1	AT5G54490	41.129	124	72	1	1	123	1	124	1.46e-29	103

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAAGCTTCTCTGCCATAAT+TGG	0.174956	8.2:-70772053	None:intergenic
TGAAGACTTGCTCCCAATTA+TGG	0.261701	8.2:+70772040	MS.gene067296:CDS
TGATAACAAGTGAGAGTCTT+AGG	0.273916	8.2:+70772141	MS.gene067296:CDS
TTGTTATCAAACCTGTTTCT+TGG	0.291271	8.2:-70772127	None:intergenic
AAATTCTGCTTCCATGGTTG+TGG	0.331149	8.2:-70772018	None:intergenic
CTGTTTCTTGGTCTGCTAGA+AGG	0.333027	8.2:-70772115	None:intergenic
TCAGCATCTTCCATCATTCC+TGG	0.349974	8.2:-70772296	None:intergenic
AATTCTGCTTCCATGGTTGT+GGG	0.389073	8.2:-70772017	None:intergenic
GGTTTGCTTCATGTTCTTGT+TGG	0.413426	8.2:-70771991	None:intergenic
CTTCTAGCAGACCAAGAAAC+AGG	0.415817	8.2:+70772116	MS.gene067296:CDS
GTCTTCAAATTCTGCTTCCA+TGG	0.425599	8.2:-70772024	None:intergenic
CAAGCTTCTCTGCCATAATT+GGG	0.477535	8.2:-70772052	None:intergenic
TAAAGAAGAAGCAGAAGCTA+TGG	0.478731	8.2:+70772199	MS.gene067296:CDS
TTCTGCTTCCATGGTTGTGG+GGG	0.488591	8.2:-70772015	None:intergenic
AAACTCTGCCATGCTTGGTA+TGG	0.490418	8.2:+70772166	MS.gene067296:CDS
AAGGCTTAGTCCAGGAATGA+TGG	0.501555	8.2:+70772286	MS.gene067296:CDS
GTTAAACAAGGTGATCTTGA+TGG	0.522373	8.2:+70772221	MS.gene067296:CDS
TCTGCCATGCTTGGTATGGA+AGG	0.538431	8.2:+70772170	MS.gene067296:CDS
TTGGTTGGAGAAAGCAATTG+AGG	0.545757	8.2:+70772322	MS.gene067296:CDS
CAAGGTGATCTTGATGGTGA+TGG	0.548309	8.2:+70772227	MS.gene067296:CDS
TGATGGAAGATGCTGAAACT+TGG	0.556410	8.2:+70772303	MS.gene067296:CDS
GGAAGATGCTGAAACTTGGT+TGG	0.562957	8.2:+70772307	MS.gene067296:CDS
ATTCTGCTTCCATGGTTGTG+GGG	0.572700	8.2:-70772016	None:intergenic
ACCACATAGTTCAGATACAA+AGG	0.600386	8.2:-70772084	None:intergenic
CATTCCTTCCATACCAAGCA+TGG	0.612253	8.2:-70772174	None:intergenic
TTGATGGTAAGGCTTAGTCC+AGG	0.627765	8.2:+70772278	MS.gene067296:CDS
TTTGTATCTGAACTATGTGG+TGG	0.640181	8.2:+70772086	MS.gene067296:CDS
GCAGAAGCTATGGTTAAACA+AGG	0.656352	8.2:+70772209	MS.gene067296:CDS
ACCTTTGTATCTGAACTATG+TGG	0.695403	8.2:+70772083	MS.gene067296:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTGCATATTGATGGTA+AGG	+	chr8.2:70772267-70772286	MS.gene067296:CDS	25.0%
!	GACTGAATTTTGCATATTGA+TGG	+	chr8.2:70772262-70772281	MS.gene067296:CDS	30.0%
!	TTGTTATCAAACCTGTTTCT+TGG	-	chr8.2:70772130-70772149	None:intergenic	30.0%
	ACCACATAGTTCAGATACAA+AGG	-	chr8.2:70772087-70772106	None:intergenic	35.0%
	ACCTTTGTATCTGAACTATG+TGG	+	chr8.2:70772083-70772102	MS.gene067296:CDS	35.0%
	GTTAAACAAGGTGATCTTGA+TGG	+	chr8.2:70772221-70772240	MS.gene067296:CDS	35.0%
	TAAAGAAGAAGCAGAAGCTA+TGG	+	chr8.2:70772199-70772218	MS.gene067296:CDS	35.0%
	TGATAACAAGTGAGAGTCTT+AGG	+	chr8.2:70772141-70772160	MS.gene067296:CDS	35.0%
	TTTGTATCTGAACTATGTGG+TGG	+	chr8.2:70772086-70772105	MS.gene067296:CDS	35.0%
	AAATTCTGCTTCCATGGTTG+TGG	-	chr8.2:70772021-70772040	None:intergenic	40.0%
	AATTCTGCTTCCATGGTTGT+GGG	-	chr8.2:70772020-70772039	None:intergenic	40.0%
	CAAGCTTCTCTGCCATAATT+GGG	-	chr8.2:70772055-70772074	None:intergenic	40.0%
	GCAGAAGCTATGGTTAAACA+AGG	+	chr8.2:70772209-70772228	MS.gene067296:CDS	40.0%
	GTCTTCAAATTCTGCTTCCA+TGG	-	chr8.2:70772027-70772046	None:intergenic	40.0%
	TCAAGCTTCTCTGCCATAAT+TGG	-	chr8.2:70772056-70772075	None:intergenic	40.0%
	TGATGGAAGATGCTGAAACT+TGG	+	chr8.2:70772303-70772322	MS.gene067296:CDS	40.0%
	TTGGTTGGAGAAAGCAATTG+AGG	+	chr8.2:70772322-70772341	MS.gene067296:CDS	40.0%
!	GGTTTGCTTCATGTTCTTGT+TGG	-	chr8.2:70771994-70772013	None:intergenic	40.0%
!	TGAAGACTTGCTCCCAATTA+TGG	+	chr8.2:70772040-70772059	MS.gene067296:CDS	40.0%
	AAACTCTGCCATGCTTGGTA+TGG	+	chr8.2:70772166-70772185	MS.gene067296:CDS	45.0%
	AAGGCTTAGTCCAGGAATGA+TGG	+	chr8.2:70772286-70772305	MS.gene067296:CDS	45.0%
	AGGAAAAACTCTGCCATGCT+TGG	+	chr8.2:70772161-70772180	MS.gene067296:CDS	45.0%
	ATTCTGCTTCCATGGTTGTG+GGG	-	chr8.2:70772019-70772038	None:intergenic	45.0%
	CATTCCTTCCATACCAAGCA+TGG	-	chr8.2:70772177-70772196	None:intergenic	45.0%
	CTTCTAGCAGACCAAGAAAC+AGG	+	chr8.2:70772116-70772135	MS.gene067296:CDS	45.0%
	GGAAGATGCTGAAACTTGGT+TGG	+	chr8.2:70772307-70772326	MS.gene067296:CDS	45.0%
	TCAGCATCTTCCATCATTCC+TGG	-	chr8.2:70772299-70772318	None:intergenic	45.0%
!	CTGTTTCTTGGTCTGCTAGA+AGG	-	chr8.2:70772118-70772137	None:intergenic	45.0%
!	TTGATGGTAAGGCTTAGTCC+AGG	+	chr8.2:70772278-70772297	MS.gene067296:CDS	45.0%
!!	CAAGGTGATCTTGATGGTGA+TGG	+	chr8.2:70772227-70772246	MS.gene067296:CDS	45.0%
	TCTGCCATGCTTGGTATGGA+AGG	+	chr8.2:70772170-70772189	MS.gene067296:CDS	50.0%
	TTCTGCTTCCATGGTTGTGG+GGG	-	chr8.2:70772018-70772037	None:intergenic	50.0%
	GCAAACCACCCCCACAACCA+TGG	+	chr8.2:70772007-70772026	MS.gene067296:CDS	60.0%
	TGCTTCCATGGTTGTGGGGG+TGG	-	chr8.2:70772015-70772034	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	70771984	70772361	70771984	ID=MS.gene067296
chr8.2	mRNA	70771984	70772361	70771984	ID=MS.gene067296.t1;Parent=MS.gene067296
chr8.2	exon	70771984	70772361	70771984	ID=MS.gene067296.t1.exon1;Parent=MS.gene067296.t1
chr8.2	CDS	70771984	70772361	70771984	ID=cds.MS.gene067296.t1;Parent=MS.gene067296.t1

Gene Sequence	>MS.gene067296 ATGGAAACCAACAAGAACATGAAGCAAACCACCCCCACAACCATGGAAGCAGAATTTGAAGACTTGCTCCCAATTATGGCAGAGAAGCTTGATGTAGAAACCTTTGTATCTGAACTATGTGGTGGTTTTAACCTTCTAGCAGACCAAGAAACAGGTTTGATAACAAGTGAGAGTCTTAGGAAAAACTCTGCCATGCTTGGTATGGAAGGAATGAGTAAAGAAGAAGCAGAAGCTATGGTTAAACAAGGTGATCTTGATGGTGATGGAAAGCTGAATGAGACTGAATTTTGCATATTGATGGTAAGGCTTAGTCCAGGAATGATGGAAGATGCTGAAACTTGGTTGGAGAAAGCAATTGAGGAACAGCTAAGAAATTAA
Protein sequence	>MS.gene067296.t1 METNKNMKQTTPTTMEAEFEDLLPIMAEKLDVETFVSELCGGFNLLADQETGLITSESLRKNSAMLGMEGMSKEEAEAMVKQGDLDGDGKLNETEFCILMVRLSPGMMEDAETWLEKAIEEQLRN