Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060524.t1 | XP_003627531.1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 4.20E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060524.t1 | Q9ZPX9 | 66.9 | 124 | 41 | 0 | 2 | 125 | 5 | 128 | 6.4e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060524.t1 | G7LAU8 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 3.0e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90533 | MS.gene060524 | PPI |
| MS.gene000304 | MS.gene060524 | PPI |
| MS.gene31568 | MS.gene060524 | PPI |
| MS.gene060524 | MS.gene35762 | PPI |
| MS.gene060524 | MS.gene032094 | PPI |
| MS.gene060524 | MS.gene068805 | PPI |
| MS.gene060524 | MS.gene51384 | PPI |
| MS.gene031332 | MS.gene060524 | PPI |
| MS.gene060524 | MS.gene25536 | PPI |
| MS.gene060524 | MS.gene45281 | PPI |
| MS.gene064243 | MS.gene060524 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060524.t1 | MTR_8g027180 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 7.36e-87 | 248 |
| MS.gene060524.t1 | MTR_8g466250 | 49.138 | 116 | 56 | 1 | 8 | 123 | 3 | 115 | 1.51e-36 | 121 |
| MS.gene060524.t1 | MTR_2g081580 | 47.273 | 110 | 58 | 0 | 16 | 125 | 7 | 116 | 1.62e-36 | 121 |
| MS.gene060524.t1 | MTR_2g011400 | 57.426 | 101 | 43 | 0 | 19 | 119 | 14 | 114 | 3.87e-36 | 120 |
| MS.gene060524.t1 | MTR_2g089210 | 53.271 | 107 | 50 | 0 | 18 | 124 | 9 | 115 | 5.20e-36 | 119 |
| MS.gene060524.t1 | MTR_1g052885 | 52.381 | 105 | 50 | 0 | 19 | 123 | 6 | 110 | 5.43e-35 | 117 |
| MS.gene060524.t1 | MTR_2g081300 | 55.789 | 95 | 41 | 1 | 15 | 109 | 1 | 94 | 1.43e-29 | 103 |
| MS.gene060524.t1 | MTR_2g081440 | 55.319 | 94 | 41 | 1 | 16 | 109 | 6 | 98 | 8.89e-29 | 101 |
| MS.gene060524.t1 | MTR_2g081350 | 51.546 | 97 | 46 | 1 | 18 | 114 | 8 | 103 | 1.30e-27 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060524.t1 | AT2G46600 | 68.908 | 119 | 37 | 0 | 7 | 125 | 10 | 128 | 1.76e-57 | 174 |
| MS.gene060524.t1 | AT4G27280 | 44.355 | 124 | 68 | 1 | 1 | 123 | 1 | 124 | 3.74e-32 | 110 |
| MS.gene060524.t1 | AT5G54490 | 41.129 | 124 | 72 | 1 | 1 | 123 | 1 | 124 | 1.46e-29 | 103 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATCACAAGTGAGAGTCTT+AGG | 0.282974 | 8.1:+75159073 | MS.gene060524:CDS |
| TTGTGATCAAACCTGTTTCT+TGG | 0.291794 | 8.1:-75159059 | None:intergenic |
| AAATTCTGCTTCCATGGTTG+TGG | 0.331149 | 8.1:-75158950 | None:intergenic |
| TCAGCATCTTCCATCATTCC+AGG | 0.368038 | 8.1:-75159228 | None:intergenic |
| TCAAGCTTCTCTGCCATGAT+TGG | 0.375958 | 8.1:-75158985 | None:intergenic |
| AATTCTGCTTCCATGGTTGT+GGG | 0.389073 | 8.1:-75158949 | None:intergenic |
| CTTCTAGCAGACCAAGAAAC+AGG | 0.407080 | 8.1:+75159048 | MS.gene060524:CDS |
| GGTTTGCTTCATGTTCTTGT+TGG | 0.413426 | 8.1:-75158923 | None:intergenic |
| GTCTTCAAATTCTGCTTCCA+TGG | 0.425599 | 8.1:-75158956 | None:intergenic |
| TGAAGACTTGCTCCCAATCA+TGG | 0.429133 | 8.1:+75158972 | MS.gene060524:CDS |
| TAAAGAAGAAGCAGAAGCTA+TGG | 0.478731 | 8.1:+75159131 | MS.gene060524:CDS |
| CAAGCTTCTCTGCCATGATT+GGG | 0.483048 | 8.1:-75158984 | None:intergenic |
| TTCTGCTTCCATGGTTGTGG+GGG | 0.488591 | 8.1:-75158947 | None:intergenic |
| AAACTCAGCCATGCTTGGTA+TGG | 0.496241 | 8.1:+75159098 | MS.gene060524:CDS |
| AAGGCTTAGTCCTGGAATGA+TGG | 0.496275 | 8.1:+75159218 | MS.gene060524:CDS |
| GTTAAACAAGGTGATCTTGA+TGG | 0.522373 | 8.1:+75159153 | MS.gene060524:CDS |
| TCAGCCATGCTTGGTATGGA+AGG | 0.540928 | 8.1:+75159102 | MS.gene060524:CDS |
| TTGGTTGGAGAAAGCAATTG+AGG | 0.545757 | 8.1:+75159254 | MS.gene060524:CDS |
| CAAGGTGATCTTGATGGTGA+TGG | 0.548309 | 8.1:+75159159 | MS.gene060524:CDS |
| TGATGGAAGATGCTGAAACT+TGG | 0.556410 | 8.1:+75159235 | MS.gene060524:CDS |
| GGAAGATGCTGAAACTTGGT+TGG | 0.562957 | 8.1:+75159239 | MS.gene060524:CDS |
| ATTCTGCTTCCATGGTTGTG+GGG | 0.572700 | 8.1:-75158948 | None:intergenic |
| TTGATGGTAAGGCTTAGTCC+TGG | 0.584659 | 8.1:+75159210 | MS.gene060524:CDS |
| ACCACATAGTTCAGATACAA+AGG | 0.600386 | 8.1:-75159016 | None:intergenic |
| CATTCCTTCCATACCAAGCA+TGG | 0.606430 | 8.1:-75159106 | None:intergenic |
| TTTGTATCTGAACTATGTGG+TGG | 0.640181 | 8.1:+75159018 | MS.gene060524:CDS |
| GCAGAAGCTATGGTTAAACA+AGG | 0.656352 | 8.1:+75159141 | MS.gene060524:CDS |
| ACCTTTGTATCTGAACTATG+TGG | 0.695403 | 8.1:+75159015 | MS.gene060524:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTTGCATATTGATGGTA+AGG | + | chr8.1:75159199-75159218 | MS.gene060524:CDS | 30.0% |
| ACCACATAGTTCAGATACAA+AGG | - | chr8.1:75159019-75159038 | None:intergenic | 35.0% | |
| ACCTTTGTATCTGAACTATG+TGG | + | chr8.1:75159015-75159034 | MS.gene060524:CDS | 35.0% | |
| GTTAAACAAGGTGATCTTGA+TGG | + | chr8.1:75159153-75159172 | MS.gene060524:CDS | 35.0% | |
| TAAAGAAGAAGCAGAAGCTA+TGG | + | chr8.1:75159131-75159150 | MS.gene060524:CDS | 35.0% | |
| TTTGTATCTGAACTATGTGG+TGG | + | chr8.1:75159018-75159037 | MS.gene060524:CDS | 35.0% | |
| ! | GACTGAGTTTTGCATATTGA+TGG | + | chr8.1:75159194-75159213 | MS.gene060524:CDS | 35.0% |
| ! | TTGTGATCAAACCTGTTTCT+TGG | - | chr8.1:75159062-75159081 | None:intergenic | 35.0% |
| AAATTCTGCTTCCATGGTTG+TGG | - | chr8.1:75158953-75158972 | None:intergenic | 40.0% | |
| AATTCTGCTTCCATGGTTGT+GGG | - | chr8.1:75158952-75158971 | None:intergenic | 40.0% | |
| GCAGAAGCTATGGTTAAACA+AGG | + | chr8.1:75159141-75159160 | MS.gene060524:CDS | 40.0% | |
| GTCTTCAAATTCTGCTTCCA+TGG | - | chr8.1:75158959-75158978 | None:intergenic | 40.0% | |
| TGATCACAAGTGAGAGTCTT+AGG | + | chr8.1:75159073-75159092 | MS.gene060524:CDS | 40.0% | |
| TGATGGAAGATGCTGAAACT+TGG | + | chr8.1:75159235-75159254 | MS.gene060524:CDS | 40.0% | |
| TTGGTTGGAGAAAGCAATTG+AGG | + | chr8.1:75159254-75159273 | MS.gene060524:CDS | 40.0% | |
| ! | GGTTTGCTTCATGTTCTTGT+TGG | - | chr8.1:75158926-75158945 | None:intergenic | 40.0% |
| AAACTCAGCCATGCTTGGTA+TGG | + | chr8.1:75159098-75159117 | MS.gene060524:CDS | 45.0% | |
| AAGGCTTAGTCCTGGAATGA+TGG | + | chr8.1:75159218-75159237 | MS.gene060524:CDS | 45.0% | |
| AGGAAAAACTCAGCCATGCT+TGG | + | chr8.1:75159093-75159112 | MS.gene060524:CDS | 45.0% | |
| ATTCTGCTTCCATGGTTGTG+GGG | - | chr8.1:75158951-75158970 | None:intergenic | 45.0% | |
| CAAGCTTCTCTGCCATGATT+GGG | - | chr8.1:75158987-75159006 | None:intergenic | 45.0% | |
| CATTCCTTCCATACCAAGCA+TGG | - | chr8.1:75159109-75159128 | None:intergenic | 45.0% | |
| CTTCTAGCAGACCAAGAAAC+AGG | + | chr8.1:75159048-75159067 | MS.gene060524:CDS | 45.0% | |
| GGAAGATGCTGAAACTTGGT+TGG | + | chr8.1:75159239-75159258 | MS.gene060524:CDS | 45.0% | |
| TCAAGCTTCTCTGCCATGAT+TGG | - | chr8.1:75158988-75159007 | None:intergenic | 45.0% | |
| TCAGCATCTTCCATCATTCC+AGG | - | chr8.1:75159231-75159250 | None:intergenic | 45.0% | |
| ! | TGAAGACTTGCTCCCAATCA+TGG | + | chr8.1:75158972-75158991 | MS.gene060524:CDS | 45.0% |
| ! | TTGATGGTAAGGCTTAGTCC+TGG | + | chr8.1:75159210-75159229 | MS.gene060524:CDS | 45.0% |
| !! | CAAGGTGATCTTGATGGTGA+TGG | + | chr8.1:75159159-75159178 | MS.gene060524:CDS | 45.0% |
| TCAGCCATGCTTGGTATGGA+AGG | + | chr8.1:75159102-75159121 | MS.gene060524:CDS | 50.0% | |
| TTCTGCTTCCATGGTTGTGG+GGG | - | chr8.1:75158950-75158969 | None:intergenic | 50.0% | |
| GCAAACCACCCCCACAACCA+TGG | + | chr8.1:75158939-75158958 | MS.gene060524:CDS | 60.0% | |
| TGCTTCCATGGTTGTGGGGG+TGG | - | chr8.1:75158947-75158966 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 75158916 | 75159293 | 75158916 | ID=MS.gene060524 |
| chr8.1 | mRNA | 75158916 | 75159293 | 75158916 | ID=MS.gene060524.t1;Parent=MS.gene060524 |
| chr8.1 | exon | 75158916 | 75159293 | 75158916 | ID=MS.gene060524.t1.exon1;Parent=MS.gene060524.t1 |
| chr8.1 | CDS | 75158916 | 75159293 | 75158916 | ID=cds.MS.gene060524.t1;Parent=MS.gene060524.t1 |
| Gene Sequence |
| Protein sequence |
