Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069092.t1 | XP_003612898.1 | 90 | 120 | 12 | 0 | 1 | 120 | 1 | 120 | 8.20E-30 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069092.t1 | G7KF21 | 90.0 | 120 | 12 | 0 | 1 | 120 | 1 | 120 | 5.9e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene069092 | 0.802203 | 6.27E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene069092 | -0.800998 | 1.11E-48 | -1.69E-46 |
| MS.gene054183 | MS.gene069092 | 0.801067 | 1.07E-48 | -1.69E-46 |
| MS.gene054930 | MS.gene069092 | 0.839783 | 1.29E-57 | -1.69E-46 |
| MS.gene056723 | MS.gene069092 | 0.824627 | 7.29E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene069092 | 0.801624 | 8.25E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene069092 | 0.835811 | 1.35E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069092.t1 | MTR_5g430430 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 9.34e-80 | 230 |
| MS.gene069092.t1 | MTR_5g030290 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 9.34e-80 | 230 |
| MS.gene069092.t1 | MTR_5g030310 | 84.167 | 120 | 19 | 0 | 1 | 120 | 1 | 120 | 5.16e-69 | 203 |
| MS.gene069092.t1 | MTR_5g430440 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 5.77e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTCTTGAGTGCAGCTT+TGG | 0.250449 | 5.3:+19132896 | None:intergenic |
| TCACTGCTACTCTCAATTTC+AGG | 0.257468 | 5.3:+19132054 | None:intergenic |
| GAAGGCCCTGATGCAGATTC+AGG | 0.346257 | 5.3:-19131970 | MS.gene069092:CDS |
| TTGCTCTTGAGTGCAGCTTT+GGG | 0.361214 | 5.3:+19132897 | None:intergenic |
| CCCTTGCCTGAATCTGCATC+AGG | 0.393096 | 5.3:+19131964 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.472681 | 5.3:-19132690 | MS.gene069092:CDS |
| TGTGTTATAACTAGGACTAC+TGG | 0.487878 | 5.3:-19132654 | MS.gene069092:CDS |
| TCTTGTGCATGCAGTGATGA+TGG | 0.527741 | 5.3:-19132087 | MS.gene069092:intron |
| AGGACTACTGGACCTGATGC+TGG | 0.536873 | 5.3:-19132642 | MS.gene069092:CDS |
| CAAAGCTGCACTCAAGAGCA+AGG | 0.561178 | 5.3:-19132895 | MS.gene069092:CDS |
| CCCTGATGCAGATTCAGGCA+AGG | 0.568742 | 5.3:-19131965 | MS.gene069092:CDS |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.3:-19132662 | MS.gene069092:CDS |
| TCTGCAAGTGTGGTGAAGGA+TGG | 0.591787 | 5.3:-19132014 | MS.gene069092:CDS |
| CCTTGCCTGAATCTGCATCA+GGG | 0.592747 | 5.3:+19131965 | None:intergenic |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.593456 | 5.3:-19132018 | MS.gene069092:CDS |
| CCTGATGCAGATTCAGGCAA+GGG | 0.596736 | 5.3:-19131964 | MS.gene069092:CDS |
| GTGTGTGACATACAGTGTGC+AGG | 0.603372 | 5.3:+19132587 | None:intergenic |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.620823 | 5.3:-19132048 | MS.gene069092:CDS |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5.3:-19132684 | MS.gene069092:CDS |
| AAAGCTGCACTCAAGAGCAA+GGG | 0.647133 | 5.3:-19132894 | MS.gene069092:intron |
| ACAACACATTGCCCAAACCC+AGG | 0.649057 | 5.3:+19132700 | None:intergenic |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5.3:-19132680 | MS.gene069092:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.3:-19132024 | MS.gene069092:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAATATAATTCATTTT+TGG | - | chr5.3:19132669-19132688 | MS.gene069092:CDS | 10.0% |
| !! | AAAACTATATAGATGAAATA+AGG | + | chr5.3:19131981-19132000 | None:intergenic | 15.0% |
| !!! | TTCATTTTTTAGATTGAATT+GGG | - | chr5.3:19132429-19132448 | MS.gene069092:intron | 15.0% |
| !!! | TTTCATTTTTTAGATTGAAT+TGG | - | chr5.3:19132428-19132447 | MS.gene069092:intron | 15.0% |
| !! | ATTAACTCTTGAGATAAAAT+CGG | - | chr5.3:19132599-19132618 | MS.gene069092:intron | 20.0% |
| !! | GATGGTAGTAAAATTAAAAT+AGG | + | chr5.3:19132272-19132291 | None:intergenic | 20.0% |
| !! | GATTTATTTATGCTTGAAAA+TGG | + | chr5.3:19132045-19132064 | None:intergenic | 20.0% |
| !! | GTAAAATTAAAATAGGCAAA+AGG | + | chr5.3:19132265-19132284 | None:intergenic | 20.0% |
| !!! | AGTTAATTCTGAAAAACAAT+AGG | + | chr5.3:19132586-19132605 | None:intergenic | 20.0% |
| !!! | TTTTTCAAAATCGAATGAAT+TGG | - | chr5.3:19132339-19132358 | MS.gene069092:intron | 20.0% |
| ! | TAAATATAAGCCTAGATTTG+TGG | + | chr5.3:19132650-19132669 | None:intergenic | 25.0% |
| ! | TGTTGTAACAATAGTTGAAT+CGG | + | chr5.3:19132537-19132556 | None:intergenic | 25.0% |
| ! | TTTGATCAAAATCATTTCGA+TGG | + | chr5.3:19132290-19132309 | None:intergenic | 25.0% |
| AATGAAACTGCAAAGTAACA+AGG | + | chr5.3:19132415-19132434 | None:intergenic | 30.0% | |
| AGATTTGTGGCATATTTGAA+TGG | + | chr5.3:19132637-19132656 | None:intergenic | 30.0% | |
| ATAGTTCAAGACAAAAACTC+AGG | - | chr5.3:19132099-19132118 | MS.gene069092:intron | 30.0% | |
| ATCTAGCAGTGCAATATATA+TGG | + | chr5.3:19132705-19132724 | None:intergenic | 30.0% | |
| TCAAAATCGAATGAATTGGT+TGG | - | chr5.3:19132343-19132362 | MS.gene069092:intron | 30.0% | |
| TTCAAATATGCCACAAATCT+AGG | - | chr5.3:19132637-19132656 | MS.gene069092:CDS | 30.0% | |
| ! | AAATGGAACATGAAGAGTAT+TGG | + | chr5.3:19132028-19132047 | None:intergenic | 30.0% |
| ! | GGCAAAGTTTTTGTTAAATG+CGG | - | chr5.3:19132201-19132220 | MS.gene069092:intron | 30.0% |
| AACAAGGATCAAATCGATTG+AGG | + | chr5.3:19132399-19132418 | None:intergenic | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5.3:19132160-19132179 | MS.gene069092:intron | 35.0% | |
| TCTGAAAAACAATAGGACTC+TGG | + | chr5.3:19132579-19132598 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTAC+TGG | - | chr5.3:19132168-19132187 | MS.gene069092:intron | 35.0% | |
| ! | AATAGGACTCTGGTTCAAAT+CGG | + | chr5.3:19132569-19132588 | None:intergenic | 35.0% |
| ! | AATGAATTGGTTGGTTCAAC+TGG | - | chr5.3:19132352-19132371 | MS.gene069092:intron | 35.0% |
| ! | TAAACCAATAAGCTTTGTGC+AGG | - | chr5.3:19132070-19132089 | MS.gene069092:CDS | 35.0% |
| !! | GATGGAGTTGTTCCATTTTT+AGG | - | chr5.3:19132826-19132845 | MS.gene069092:intron | 35.0% |
| !!! | GCGCAACTAATTGTCATTTT+AGG | - | chr5.3:19132472-19132491 | MS.gene069092:intron | 35.0% |
| !!! | TGTTCCATTTTTAGGACTGA+AGG | - | chr5.3:19132834-19132853 | MS.gene069092:intron | 35.0% |
| ACAAAAACTTTGCCAGCATC+AGG | + | chr5.3:19132195-19132214 | None:intergenic | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5.3:19132798-19132817 | MS.gene069092:intron | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5.3:19132774-19132793 | MS.gene069092:intron | 40.0% | |
| CTTTCCTGCACAAAGCTTAT+TGG | + | chr5.3:19132077-19132096 | None:intergenic | 40.0% | |
| TCACTGCTACTCTCAATTTC+AGG | + | chr5.3:19132771-19132790 | None:intergenic | 40.0% | |
| AAAAACTCAGGCCTGGGTTT+GGG | - | chr5.3:19132111-19132130 | MS.gene069092:intron | 45.0% | |
| AAAGCTGCACTCAAGAGCAA+GGG | - | chr5.3:19131928-19131947 | MS.gene069092:CDS | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5.3:19132804-19132823 | MS.gene069092:intron | 45.0% | |
| AGGGCCTTCAGTCCTAAAAA+TGG | + | chr5.3:19132841-19132860 | None:intergenic | 45.0% | |
| CAAGACAAAAACTCAGGCCT+GGG | - | chr5.3:19132105-19132124 | MS.gene069092:intron | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | chr5.3:19132138-19132157 | MS.gene069092:intron | 45.0% | |
| TCAAGACAAAAACTCAGGCC+TGG | - | chr5.3:19132104-19132123 | MS.gene069092:intron | 45.0% | |
| TCTTGTGCATGCAGTGATGA+TGG | - | chr5.3:19132735-19132754 | MS.gene069092:intron | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | chr5.3:19132142-19132161 | MS.gene069092:intron | 45.0% | |
| TTGCTCTTGAGTGCAGCTTT+GGG | + | chr5.3:19131928-19131947 | None:intergenic | 45.0% | |
| ACAACACATTGCCCAAACCC+AGG | + | chr5.3:19132125-19132144 | None:intergenic | 50.0% | |
| CAAAAACTCAGGCCTGGGTT+TGG | - | chr5.3:19132110-19132129 | MS.gene069092:intron | 50.0% | |
| CAAAGCTGCACTCAAGAGCA+AGG | - | chr5.3:19131927-19131946 | MS.gene069092:CDS | 50.0% | |
| CCTGATGCAGATTCAGGCAA+GGG | - | chr5.3:19132858-19132877 | MS.gene069092:intron | 50.0% | |
| CCTTGCCTGAATCTGCATCA+GGG | + | chr5.3:19132860-19132879 | None:intergenic | 50.0% | |
| CTTGCTCTTGAGTGCAGCTT+TGG | + | chr5.3:19131929-19131948 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | chr5.3:19132132-19132151 | MS.gene069092:intron | 50.0% | |
| GTGTGTGACATACAGTGTGC+AGG | + | chr5.3:19132238-19132257 | None:intergenic | 50.0% | |
| TCTGCAAGTGTGGTGAAGGA+TGG | - | chr5.3:19132808-19132827 | MS.gene069092:intron | 50.0% | |
| AGGACTACTGGACCTGATGC+TGG | - | chr5.3:19132180-19132199 | MS.gene069092:intron | 55.0% | |
| CCCTGATGCAGATTCAGGCA+AGG | - | chr5.3:19132857-19132876 | MS.gene069092:intron | 55.0% | |
| CCCTTGCCTGAATCTGCATC+AGG | + | chr5.3:19132861-19132880 | None:intergenic | 55.0% | |
| GAAGGCCCTGATGCAGATTC+AGG | - | chr5.3:19132852-19132871 | MS.gene069092:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19131918 | 19132926 | 19131918 | ID=MS.gene069092 |
| chr5.3 | mRNA | 19131918 | 19132926 | 19131918 | ID=MS.gene069092.t1;Parent=MS.gene069092 |
| chr5.3 | exon | 19132895 | 19132926 | 19132895 | ID=MS.gene069092.t1.exon1;Parent=MS.gene069092.t1 |
| chr5.3 | CDS | 19132895 | 19132926 | 19132895 | ID=cds.MS.gene069092.t1;Parent=MS.gene069092.t1 |
| chr5.3 | exon | 19132600 | 19132752 | 19132600 | ID=MS.gene069092.t1.exon2;Parent=MS.gene069092.t1 |
| chr5.3 | CDS | 19132600 | 19132752 | 19132600 | ID=cds.MS.gene069092.t1;Parent=MS.gene069092.t1 |
| chr5.3 | exon | 19131918 | 19132095 | 19131918 | ID=MS.gene069092.t1.exon3;Parent=MS.gene069092.t1 |
| chr5.3 | CDS | 19131918 | 19132095 | 19131918 | ID=cds.MS.gene069092.t1;Parent=MS.gene069092.t1 |
| Gene Sequence |
| Protein sequence |
