AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069194


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069194.t1 MTR_5g030500 98.140 215 2 1 1 215 1 213 6.13e-158 436
MS.gene069194.t1 MTR_1g016480 55.429 175 72 3 33 206 26 195 3.98e-66 202
MS.gene069194.t1 MTR_3g071740 53.293 167 72 2 49 215 69 229 5.36e-62 193
MS.gene069194.t1 MTR_5g083270 49.505 202 87 5 16 208 8 203 2.35e-61 191
MS.gene069194.t1 MTR_4g014460 54.217 166 72 2 46 211 49 210 3.51e-56 177
MS.gene069194.t1 MTR_7g070050 58.788 165 65 2 46 208 13 176 3.40e-53 169
MS.gene069194.t1 MTR_8g027805 45.856 181 90 4 30 208 1 175 2.56e-49 159
MS.gene069194.t1 MTR_3g090980 48.795 166 78 3 45 209 20 179 3.18e-49 159
MS.gene069194.t1 MTR_1g028380 49.398 166 77 3 45 209 18 177 8.45e-49 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069194.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.05e-91 268
MS.gene069194.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.05e-91 268
MS.gene069194.t1 AT4G17870 69.022 184 54 1 30 213 1 181 2.20e-90 264
MS.gene069194.t1 AT2G26040 57.558 172 71 2 33 204 9 178 6.04e-68 207
MS.gene069194.t1 AT1G73000 52.432 185 82 3 31 209 22 206 2.84e-66 203
MS.gene069194.t1 AT5G05440 54.938 162 67 2 46 207 45 200 4.60e-61 190
MS.gene069194.t1 AT2G40330 51.534 163 78 1 50 212 52 213 3.29e-57 180
MS.gene069194.t1 AT2G38310 58.491 159 60 1 50 208 43 195 4.15e-52 167
MS.gene069194.t1 AT4G01026 51.250 160 71 3 50 208 27 180 3.06e-50 162
MS.gene069194.t1 AT5G53160 48.780 164 79 2 45 208 18 176 1.08e-49 160
MS.gene069194.t1 AT1G01360 47.904 167 80 3 43 208 18 178 1.67e-49 160
MS.gene069194.t1 AT4G27920 47.879 165 80 3 45 208 13 172 9.40e-49 157
MS.gene069194.t1 AT5G45860 54.000 150 64 2 59 208 10 154 4.34e-39 132
MS.gene069194.t1 AT5G45870 52.667 150 65 2 59 208 10 153 1.12e-38 131
MS.gene069194.t1 AT4G18620 48.408 157 73 3 57 208 7 160 1.19e-33 119
MS.gene069194.t1 AT5G53160 50.485 103 49 1 45 147 18 118 1.24e-29 108

Find 72 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACTTCCGTTCACGGTTT+CGG 0.321183 5.3:-19613731 MS.gene069194:CDS
TGAGATCTGGACCGTTGTTT+TGG 0.357741 5.3:-19613690 MS.gene069194:CDS
GACGACGAACGTCGTGTTAC+CGG 0.369543 5.3:-19613800 MS.gene069194:CDS
CGTTCCGTTACTTCCGTTCA+CGG 0.394929 5.3:-19613737 MS.gene069194:CDS
GGAGGCCGGAGGGGAGTGTT+AGG 0.404211 5.3:+19614104 None:intergenic
ATAAAGTGTTTGTAGATTTG+CGG 0.406329 5.3:+19613935 None:intergenic
TCCTCTTCAGTATTACCTTC+AGG 0.411978 5.3:+19613647 None:intergenic
AGAAGCCTGGAGCATTGGTT+GGG 0.412638 5.3:+19614019 None:intergenic
GTGATAACGGTGGTGAGATC+TGG 0.416625 5.3:-19613703 MS.gene069194:CDS
CTCTCGGTGCTAGTCGCCGC+CGG 0.416895 5.3:+19613836 None:intergenic
ACCGTCACGGTTCTTTCCTT+CGG 0.424743 5.3:+19613565 None:intergenic
TCACACCACACCTACCTCAT+AGG 0.430563 5.3:-19614043 MS.gene069194:CDS
GGTGGAAGCGATGGCGGTGG+AGG 0.430580 5.3:+19614168 None:intergenic
GTCGGAATCTTGATCGGAGG+TGG 0.456152 5.3:+19614150 None:intergenic
ACGCGCCGCCGCAAACCGTC+TGG 0.474731 5.3:-19613982 MS.gene069194:CDS
CAATGTCATCTCCGGTCTCC+CGG 0.474823 5.3:-19613855 MS.gene069194:CDS
GCTAGTCGCCGCCGGGAGAC+CGG 0.484689 5.3:+19613844 None:intergenic
TCCGTTCACGGTTTCGGTGA+CGG 0.493319 5.3:-19613725 MS.gene069194:CDS
ACGACGGACCAGACGGTTTG+CGG 0.500991 5.3:+19613974 None:intergenic
AGACGGTTTGCGGCGGCGCG+TGG 0.502746 5.3:+19613984 None:intergenic
GGAGGTGGAAGCGATGGCGG+TGG 0.507691 5.3:+19614165 None:intergenic
GAGAAGCCTGGAGCATTGGT+TGG 0.510173 5.3:+19614018 None:intergenic
GATGCGTTGAGCGAGAAGCC+TGG 0.510312 5.3:+19614006 None:intergenic
GAAGCCTGGAGCATTGGTTG+GGG 0.513167 5.3:+19614020 None:intergenic
GAGCGAGAAGCCTGGAGCAT+TGG 0.514906 5.3:+19614014 None:intergenic
AGCATTGGTTGGGGCCTATG+AGG 0.517600 5.3:+19614029 None:intergenic
CGCGACGTCAATGTCATCTC+CGG 0.522159 5.3:-19613863 MS.gene069194:CDS
AAAAGCTGCTCTCTCAAAGA+AGG 0.522505 5.3:-19613911 MS.gene069194:CDS
GTCAAGAACGTCGAGTCTCT+CGG 0.524491 5.3:+19613820 None:intergenic
ACCGCCGATGATACTGAAAC+CGG 0.530952 5.3:+19613781 None:intergenic
GGTTTCGGTGACGGTGATAA+CGG 0.543393 5.3:-19613716 MS.gene069194:CDS
TAGGCCCCAACCAATGCTCC+AGG 0.544690 5.3:-19614024 MS.gene069194:CDS
CGGAACGGTAATTCTTCAAA+CGG 0.546020 5.3:+19613753 None:intergenic
GAATTCGTGTTGACGGAGGC+CGG 0.548211 5.3:+19614090 None:intergenic
TTGGTTGGGGCCTATGAGGT+AGG 0.550389 5.3:+19614033 None:intergenic
TTTGCGGTTTGTCGAAGCTA+CGG 0.554765 5.3:+19613951 None:intergenic
ATCGGAGGTGGAAGCGATGG+CGG 0.560121 5.3:+19614162 None:intergenic
GGGAGTCGAATTCGTGTTGA+CGG 0.560666 5.3:+19614083 None:intergenic
TCTCGGTGCTAGTCGCCGCC+GGG 0.563257 5.3:+19613837 None:intergenic
CGTGTTGACGGAGGCCGGAG+GGG 0.570765 5.3:+19614095 None:intergenic
TTGATCGGAGGTGGAAGCGA+TGG 0.571051 5.3:+19614159 None:intergenic
TTCGTGTTGACGGAGGCCGG+AGG 0.572606 5.3:+19614093 None:intergenic
ACCGTCACCGAAACCGTGAA+CGG 0.574955 5.3:+19613724 None:intergenic
TGTGGTGTGAGTTGATGAAA+GGG 0.576668 5.3:+19614056 None:intergenic
GTGACTTACCGTCACCGTCA+CGG 0.581808 5.3:+19613552 None:intergenic
TCGTGTTGACGGAGGCCGGA+GGG 0.581954 5.3:+19614094 None:intergenic
GAAACCGTGAACGGAAGTAA+CGG 0.584827 5.3:+19613733 None:intergenic
GGAAAGAACCGTGACGGTGA+CGG 0.588055 5.3:-19613560 None:intergenic
CACCACCTAACACTCCCCTC+CGG 0.591738 5.3:-19614109 MS.gene069194:CDS
GTGTTAGGTGGTGTTGTGTG+CGG 0.591821 5.3:+19614119 None:intergenic
TACGTCGTTGATGTTCCTGA+AGG 0.592427 5.3:-19613662 MS.gene069194:CDS
TTCGTCGGAATCTTGATCGG+AGG 0.592857 5.3:+19614147 None:intergenic
GTTACCGGTTTCAGTATCAT+CGG 0.595373 5.3:-19613785 MS.gene069194:CDS
GTGTGGTGTGAGTTGATGAA+AGG 0.597891 5.3:+19614055 None:intergenic
TTAGGTGGTGTTGTGTGCGG+TGG 0.600549 5.3:+19614122 None:intergenic
GGCCGGAGGGGAGTGTTAGG+TGG 0.606358 5.3:+19614107 None:intergenic
AAGCTTGCGTCTGTCACCGA+AGG 0.615885 5.3:-19613581 MS.gene069194:CDS
TGAGTTGATGAAAGGGACGA+GGG 0.616316 5.3:+19614063 None:intergenic
TCCTGAAGGTAATACTGAAG+AGG 0.617871 5.3:-19613648 MS.gene069194:CDS
GTGAGTTGATGAAAGGGACG+AGG 0.618853 5.3:+19614062 None:intergenic
TGGGGCCTATGAGGTAGGTG+TGG 0.623679 5.3:+19614038 None:intergenic
GCTACGGACGACGGACCAGA+CGG 0.637251 5.3:+19613967 None:intergenic
ACGGACCAGACGGTTTGCGG+CGG 0.644883 5.3:+19613977 None:intergenic
ACCGGTTTCAGTATCATCGG+CGG 0.669189 5.3:-19613782 MS.gene069194:CDS
ACCGAAGGAAAGAACCGTGA+CGG 0.674541 5.3:-19613566 MS.gene069194:CDS
AGTCGAATTCGTGTTGACGG+AGG 0.683583 5.3:+19614086 None:intergenic
TTTGTCGAAGCTACGGACGA+CGG 0.690180 5.3:+19613958 None:intergenic
GACGTAAGATTCCAAAACAA+CGG 0.691402 5.3:+19613679 None:intergenic
TTCGGTGACGGTGATAACGG+TGG 0.691549 5.3:-19613713 MS.gene069194:CDS
TGTCATCTCCGGTCTCCCGG+CGG 0.695228 5.3:-19613852 MS.gene069194:CDS
CGTGAACGGAAGTAACGGAA+CGG 0.712612 5.3:+19613738 None:intergenic
CTGAAGATTAAGCTTCACAA+CGG 0.714266 5.3:+19613604 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGTGTTTGTAGATTTG+CGG + chr5.3:19613813-19613832 None:intergenic 25.0%
! GAAGGTTTTCAAATGAAAGT+CGG - chr5.3:19613852-19613871 MS.gene069194:CDS 30.0%
CTGAAGATTAAGCTTCACAA+CGG + chr5.3:19614144-19614163 None:intergenic 35.0%
GACGTAAGATTCCAAAACAA+CGG + chr5.3:19614069-19614088 None:intergenic 35.0%
AAAAGCTGCTCTCTCAAAGA+AGG - chr5.3:19613834-19613853 MS.gene069194:CDS 40.0%
CGGAACGGTAATTCTTCAAA+CGG + chr5.3:19613995-19614014 None:intergenic 40.0%
TCCTCTTCAGTATTACCTTC+AGG + chr5.3:19614101-19614120 None:intergenic 40.0%
TCCTGAAGGTAATACTGAAG+AGG - chr5.3:19614097-19614116 MS.gene069194:CDS 40.0%
! GTTTTCGTCGGAATCTTGAT+CGG + chr5.3:19613604-19613623 None:intergenic 40.0%
!! GTTACCGGTTTCAGTATCAT+CGG - chr5.3:19613960-19613979 MS.gene069194:CDS 40.0%
!! TGTGGTGTGAGTTGATGAAA+GGG + chr5.3:19613692-19613711 None:intergenic 40.0%
GAAACCGTGAACGGAAGTAA+CGG + chr5.3:19614015-19614034 None:intergenic 45.0%
GTTACTTCCGTTCACGGTTT+CGG - chr5.3:19614014-19614033 MS.gene069194:CDS 45.0%
TACGTCGTTGATGTTCCTGA+AGG - chr5.3:19614083-19614102 MS.gene069194:CDS 45.0%
TTTGCGGTTTGTCGAAGCTA+CGG + chr5.3:19613797-19613816 None:intergenic 45.0%
! TGAGATCTGGACCGTTGTTT+TGG - chr5.3:19614055-19614074 MS.gene069194:CDS 45.0%
!! GTGTGGTGTGAGTTGATGAA+AGG + chr5.3:19613693-19613712 None:intergenic 45.0%
!! TGAGTTGATGAAAGGGACGA+GGG + chr5.3:19613685-19613704 None:intergenic 45.0%
ACCGAAGGAAAGAACCGTGA+CGG - chr5.3:19614179-19614198 MS.gene069194:CDS 50.0%
ACCGCCGATGATACTGAAAC+CGG + chr5.3:19613967-19613986 None:intergenic 50.0%
ACCGTCACGGTTCTTTCCTT+CGG + chr5.3:19614183-19614202 None:intergenic 50.0%
CGTTCCGTTACTTCCGTTCA+CGG - chr5.3:19614008-19614027 MS.gene069194:CDS 50.0%
GGGAGTCGAATTCGTGTTGA+CGG + chr5.3:19613665-19613684 None:intergenic 50.0%
GTCAAGAACGTCGAGTCTCT+CGG + chr5.3:19613928-19613947 None:intergenic 50.0%
TCACACCACACCTACCTCAT+AGG - chr5.3:19613702-19613721 MS.gene069194:CDS 50.0%
TTCGTCGGAATCTTGATCGG+AGG + chr5.3:19613601-19613620 None:intergenic 50.0%
TTTGTCGAAGCTACGGACGA+CGG + chr5.3:19613790-19613809 None:intergenic 50.0%
! ACCGGTTTCAGTATCATCGG+CGG - chr5.3:19613963-19613982 MS.gene069194:CDS 50.0%
! AGTCGAATTCGTGTTGACGG+AGG + chr5.3:19613662-19613681 None:intergenic 50.0%
! CGTGAACGGAAGTAACGGAA+CGG + chr5.3:19614010-19614029 None:intergenic 50.0%
! GGTTTCGGTGACGGTGATAA+CGG - chr5.3:19614029-19614048 MS.gene069194:CDS 50.0%
! TTGTGTGCGGTGGTTTTCGT+CGG + chr5.3:19613616-19613635 None:intergenic 50.0%
!! AGAAGCCTGGAGCATTGGTT+GGG + chr5.3:19613729-19613748 None:intergenic 50.0%
!! GTGAGTTGATGAAAGGGACG+AGG + chr5.3:19613686-19613705 None:intergenic 50.0%
!! GTGATAACGGTGGTGAGATC+TGG - chr5.3:19614042-19614061 MS.gene069194:CDS 50.0%
!! GTGTTAGGTGGTGTTGTGTG+CGG + chr5.3:19613629-19613648 None:intergenic 50.0%
AAGCTTGCGTCTGTCACCGA+AGG - chr5.3:19614164-19614183 MS.gene069194:CDS 55.0%
ACCGTCACCGAAACCGTGAA+CGG + chr5.3:19614024-19614043 None:intergenic 55.0%
CAATGTCATCTCCGGTCTCC+CGG - chr5.3:19613890-19613909 MS.gene069194:CDS 55.0%
CGCGACGTCAATGTCATCTC+CGG - chr5.3:19613882-19613901 MS.gene069194:CDS 55.0%
GACGACGAACGTCGTGTTAC+CGG - chr5.3:19613945-19613964 MS.gene069194:CDS 55.0%
GTCGGAATCTTGATCGGAGG+TGG + chr5.3:19613598-19613617 None:intergenic 55.0%
TCCGTTCACGGTTTCGGTGA+CGG - chr5.3:19614020-19614039 MS.gene069194:CDS 55.0%
TTGATCGGAGGTGGAAGCGA+TGG + chr5.3:19613589-19613608 None:intergenic 55.0%
TTGGTTGGGGCCTATGAGGT+AGG + chr5.3:19613715-19613734 None:intergenic 55.0%
! GAATTCGTGTTGACGGAGGC+CGG + chr5.3:19613658-19613677 None:intergenic 55.0%
!! AGCATTGGTTGGGGCCTATG+AGG + chr5.3:19613719-19613738 None:intergenic 55.0%
!! GAAGCCTGGAGCATTGGTTG+GGG + chr5.3:19613728-19613747 None:intergenic 55.0%
!! GAGAAGCCTGGAGCATTGGT+TGG + chr5.3:19613730-19613749 None:intergenic 55.0%
!! TTAGGTGGTGTTGTGTGCGG+TGG + chr5.3:19613626-19613645 None:intergenic 55.0%
!! TTCGGTGACGGTGATAACGG+TGG - chr5.3:19614032-19614051 MS.gene069194:CDS 55.0%
ACGACGGACCAGACGGTTTG+CGG + chr5.3:19613774-19613793 None:intergenic 60.0%
ATCGGAGGTGGAAGCGATGG+CGG + chr5.3:19613586-19613605 None:intergenic 60.0%
CACCACCTAACACTCCCCTC+CGG - chr5.3:19613636-19613655 MS.gene069194:CDS 60.0%
GAGCGAGAAGCCTGGAGCAT+TGG + chr5.3:19613734-19613753 None:intergenic 60.0%
GATGCGTTGAGCGAGAAGCC+TGG + chr5.3:19613742-19613761 None:intergenic 60.0%
TAGGCCCCAACCAATGCTCC+AGG - chr5.3:19613721-19613740 MS.gene069194:CDS 60.0%
TGGGGCCTATGAGGTAGGTG+TGG + chr5.3:19613710-19613729 None:intergenic 60.0%
ACGGACCAGACGGTTTGCGG+CGG + chr5.3:19613771-19613790 None:intergenic 65.0%
GCTACGGACGACGGACCAGA+CGG + chr5.3:19613781-19613800 None:intergenic 65.0%
TGTCATCTCCGGTCTCCCGG+CGG - chr5.3:19613893-19613912 MS.gene069194:CDS 65.0%
! TCGTGTTGACGGAGGCCGGA+GGG + chr5.3:19613654-19613673 None:intergenic 65.0%
! TTCGTGTTGACGGAGGCCGG+AGG + chr5.3:19613655-19613674 None:intergenic 65.0%
! CGTGTTGACGGAGGCCGGAG+GGG + chr5.3:19613653-19613672 None:intergenic 70.0%
! GGAGGTGGAAGCGATGGCGG+TGG + chr5.3:19613583-19613602 None:intergenic 70.0%
! GGTGGAAGCGATGGCGGTGG+AGG + chr5.3:19613580-19613599 None:intergenic 70.0%
!! CTCTCGGTGCTAGTCGCCGC+CGG + chr5.3:19613912-19613931 None:intergenic 70.0%
!! GGAGGCCGGAGGGGAGTGTT+AGG + chr5.3:19613644-19613663 None:intergenic 70.0%
!! GGCCGGAGGGGAGTGTTAGG+TGG + chr5.3:19613641-19613660 None:intergenic 70.0%
!! TCTCGGTGCTAGTCGCCGCC+GGG + chr5.3:19613911-19613930 None:intergenic 70.0%
ACGCGCCGCCGCAAACCGTC+TGG - chr5.3:19613763-19613782 MS.gene069194:CDS 75.0%
AGACGGTTTGCGGCGGCGCG+TGG + chr5.3:19613764-19613783 None:intergenic 75.0%
GCTAGTCGCCGCCGGGAGAC+CGG + chr5.3:19613904-19613923 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 19613561 19614206 19613561 ID=MS.gene069194
chr5.3 mRNA 19613561 19614206 19613561 ID=MS.gene069194.t1;Parent=MS.gene069194
chr5.3 exon 19613561 19614206 19613561 ID=MS.gene069194.t1.exon1;Parent=MS.gene069194.t1
chr5.3 CDS 19613561 19614206 19613561 ID=cds.MS.gene069194.t1;Parent=MS.gene069194.t1
Gene Sequence

>MS.gene069194

ATGGAGAAAGCAGAGTCCTCCACCGCCATCGCTTCCACCTCCGATCAAGATTCCGACGAAAACCACCGCACACAACACCACCTAACACTCCCCTCCGGCCTCCGTCAACACGAATTCGACTCCCTCGTCCCTTTCATCAACTCACACCACACCTACCTCATAGGCCCCAACCAATGCTCCAGGCTTCTCGCTCAACGCATCCACGCGCCGCCGCAAACCGTCTGGTCCGTCGTCCGTAGCTTCGACAAACCGCAAATCTACAAACACTTTATCAAAAGCTGCTCTCTCAAAGAAGGTTTTCAAATGAAAGTCGGTTGCACTCGCGACGTCAATGTCATCTCCGGTCTCCCGGCGGCGACTAGCACCGAGAGACTCGACGTTCTTGACGACGAACGTCGTGTTACCGGTTTCAGTATCATCGGCGGTGAACACCGTTTGAAGAATTACCGTTCCGTTACTTCCGTTCACGGTTTCGGTGACGGTGATAACGGTGGTGAGATCTGGACCGTTGTTTTGGAATCTTACGTCGTTGATGTTCCTGAAGGTAATACTGAAGAGGATACGCGTCTTTTTGCTGATACCGTTGTGAAGCTTAATCTTCAGAAGCTTGCGTCTGTCACCGAAGGAAAGAACCGTGACGGTGACG

Protein sequence

>MS.gene069194.t1

MEKAESSTAIASTSDQDSDENHRTQHHLTLPSGLRQHEFDSLVPFINSHHTYLIGPNQCSRLLAQRIHAPPQTVWSVVRSFDKPQIYKHFIKSCSLKEGFQMKVGCTRDVNVISGLPAATSTERLDVLDDERRVTGFSIIGGEHRLKNYRSVTSVHGFGDGDNGGEIWTVVLESYVVDVPEGNTEEDTRLFADTVVKLNLQKLASVTEGKNRDGD