AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054809


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054809.t1 MTR_5g030500 98.624 218 1 1 1 218 1 216 2.37e-160 442
MS.gene054809.t1 MTR_1g016480 55.429 175 72 3 33 206 26 195 5.66e-66 202
MS.gene054809.t1 MTR_3g071740 52.663 169 74 2 49 217 69 231 4.18e-62 193
MS.gene054809.t1 MTR_5g083270 49.505 202 87 5 16 208 8 203 3.34e-61 190
MS.gene054809.t1 MTR_4g014460 54.070 172 74 3 41 211 43 210 3.14e-56 178
MS.gene054809.t1 MTR_7g070050 58.788 165 65 2 46 208 13 176 3.82e-53 169
MS.gene054809.t1 MTR_8g027805 45.856 181 90 4 30 208 1 175 3.30e-49 159
MS.gene054809.t1 MTR_3g090980 48.795 166 78 3 45 209 20 179 3.45e-49 159
MS.gene054809.t1 MTR_1g028380 49.398 166 77 3 45 209 18 177 7.30e-49 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054809.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.04e-91 268
MS.gene054809.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.04e-91 268
MS.gene054809.t1 AT4G17870 69.149 188 54 2 30 216 1 185 2.14e-91 266
MS.gene054809.t1 AT2G26040 57.558 172 71 2 33 204 9 178 1.25e-67 206
MS.gene054809.t1 AT1G73000 52.432 185 82 3 31 209 22 206 2.37e-66 204
MS.gene054809.t1 AT5G05440 54.938 162 67 2 46 207 45 200 4.36e-61 190
MS.gene054809.t1 AT2G40330 51.534 163 78 1 50 212 52 213 4.04e-57 180
MS.gene054809.t1 AT2G38310 55.952 168 68 1 50 217 43 204 4.37e-52 167
MS.gene054809.t1 AT4G01026 51.250 160 71 3 50 208 27 180 3.50e-50 162
MS.gene054809.t1 AT5G53160 48.780 164 79 2 45 208 18 176 1.32e-49 160
MS.gene054809.t1 AT1G01360 47.305 167 81 3 43 208 18 178 2.90e-49 159
MS.gene054809.t1 AT4G27920 47.879 165 80 3 45 208 13 172 9.34e-49 158
MS.gene054809.t1 AT5G45860 54.000 150 64 2 59 208 10 154 6.25e-39 132
MS.gene054809.t1 AT5G45870 52.667 150 65 2 59 208 10 153 1.28e-38 131
MS.gene054809.t1 AT4G18620 48.408 157 73 3 57 208 7 160 1.44e-33 118
MS.gene054809.t1 AT5G53160 50.485 103 49 1 45 147 18 118 1.21e-29 108

Find 72 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACTTCCGTTCACGGTTT+CGG 0.321183 5.3:+19566127 MS.gene054809:CDS
TGAGATCTGGACCGTTGTTT+TGG 0.357741 5.3:+19566168 MS.gene054809:CDS
GACGACGAACGTCGTGTTAC+CGG 0.369543 5.3:+19566058 MS.gene054809:CDS
CGTTCCGTTACTTCCGTTCA+CGG 0.394929 5.3:+19566121 MS.gene054809:CDS
GGAGGCCGGAGGGGAGTGTT+AGG 0.404211 5.3:-19565754 None:intergenic
TCCTCTTCAGTATTACCTTC+AGG 0.411978 5.3:-19566211 None:intergenic
AGAAGCCTGGAGCATTGGTT+GGG 0.412638 5.3:-19565839 None:intergenic
GTGATAACGGTGGTGAGATC+TGG 0.416625 5.3:+19566155 MS.gene054809:CDS
CTCTCGGTGCTAGTCGCCGC+CGG 0.416895 5.3:-19566022 None:intergenic
ACCGTCACGGTTCTTTCCTT+CGG 0.424743 5.3:-19566293 None:intergenic
TCACACCACACCTACCTCAT+AGG 0.430563 5.3:+19565815 MS.gene054809:CDS
GGTGGAAGCGATGGCGGTGG+AGG 0.430580 5.3:-19565690 None:intergenic
ATGAAGTGTTTGTAGATTTG+CGG 0.443636 5.3:-19565923 None:intergenic
GTCGGAATCTTGATCGGAGG+TGG 0.456152 5.3:-19565708 None:intergenic
ACGCGCCGCCGCAAACCGTC+TGG 0.474731 5.3:+19565876 MS.gene054809:CDS
CAATGTCATCTCCGGTCTCC+CGG 0.474823 5.3:+19566003 MS.gene054809:CDS
GCTAGTCGCCGCCGGGAGAC+CGG 0.484689 5.3:-19566014 None:intergenic
TCCGTTCACGGTTTCGGTGA+CGG 0.493319 5.3:+19566133 MS.gene054809:CDS
ACGACGGACCAGACGGTTTG+CGG 0.500991 5.3:-19565884 None:intergenic
AGACGGTTTGCGGCGGCGCG+TGG 0.502746 5.3:-19565874 None:intergenic
AAAAGCTGCTCTCTCAAAGA+AGG 0.506128 5.3:+19565947 MS.gene054809:CDS
GGAGGTGGAAGCGATGGCGG+TGG 0.507691 5.3:-19565693 None:intergenic
GAGAAGCCTGGAGCATTGGT+TGG 0.510173 5.3:-19565840 None:intergenic
GATGCGTTGAGCGAGAAGCC+TGG 0.510312 5.3:-19565852 None:intergenic
GAAGCCTGGAGCATTGGTTG+GGG 0.513167 5.3:-19565838 None:intergenic
GAGCGAGAAGCCTGGAGCAT+TGG 0.514906 5.3:-19565844 None:intergenic
AGCATTGGTTGGGGCCTATG+AGG 0.517600 5.3:-19565829 None:intergenic
CGCGACGTCAATGTCATCTC+CGG 0.522159 5.3:+19565995 MS.gene054809:CDS
GTCAAGAACGTCGAGTCTCT+CGG 0.524491 5.3:-19566038 None:intergenic
ACCGCCGATGATACTGAAAC+CGG 0.530952 5.3:-19566077 None:intergenic
GGGAGTCGAATTCGTGTTGA+CGG 0.541010 5.3:-19565775 None:intergenic
GGTTTCGGTGACGGTGATAA+CGG 0.543393 5.3:+19566142 MS.gene054809:CDS
TAGGCCCCAACCAATGCTCC+AGG 0.544690 5.3:+19565834 MS.gene054809:CDS
CGGAACGGTAATTCTTCAAA+CGG 0.546020 5.3:-19566105 None:intergenic
GAATTCGTGTTGACGGAGGC+CGG 0.548211 5.3:-19565768 None:intergenic
TTGGTTGGGGCCTATGAGGT+AGG 0.550389 5.3:-19565825 None:intergenic
TTTGCGGTTTGTCGAAGCTA+CGG 0.554765 5.3:-19565907 None:intergenic
ATCGGAGGTGGAAGCGATGG+CGG 0.560121 5.3:-19565696 None:intergenic
TCTCGGTGCTAGTCGCCGCC+GGG 0.563257 5.3:-19566021 None:intergenic
TGTGGTGTGAGTTGATGAAA+GGG 0.564889 5.3:-19565802 None:intergenic
CGTGTTGACGGAGGCCGGAG+GGG 0.570765 5.3:-19565763 None:intergenic
TTGATCGGAGGTGGAAGCGA+TGG 0.571051 5.3:-19565699 None:intergenic
TTCGTGTTGACGGAGGCCGG+AGG 0.572606 5.3:-19565765 None:intergenic
ACCGTCACCGAAACCGTGAA+CGG 0.574955 5.3:-19566134 None:intergenic
TCGTGTTGACGGAGGCCGGA+GGG 0.581954 5.3:-19565764 None:intergenic
TGAGTTGATGAAAGGGATGA+GGG 0.584437 5.3:-19565795 None:intergenic
GAAACCGTGAACGGAAGTAA+CGG 0.584827 5.3:-19566125 None:intergenic
GGAAAGAACCGTGACGGTGA+CGG 0.588055 5.3:+19566298 MS.gene054809:CDS
CTGACTTACCGTCACCGTCA+CGG 0.588675 5.3:-19566306 None:intergenic
CACCACCTAACACTCCCCTC+CGG 0.591738 5.3:+19565749 MS.gene054809:CDS
GTGTTAGGTGGTGTTGTGTG+CGG 0.591821 5.3:-19565739 None:intergenic
TACGTCGTTGATGTTCCTGA+AGG 0.592427 5.3:+19566196 MS.gene054809:CDS
GTGAGTTGATGAAAGGGATG+AGG 0.592854 5.3:-19565796 None:intergenic
TTCGTCGGAATCTTGATCGG+AGG 0.592857 5.3:-19565711 None:intergenic
GTTACCGGTTTCAGTATCAT+CGG 0.595373 5.3:+19566073 MS.gene054809:CDS
GTGTGGTGTGAGTTGATGAA+AGG 0.597891 5.3:-19565803 None:intergenic
TTAGGTGGTGTTGTGTGCGG+TGG 0.600549 5.3:-19565736 None:intergenic
GGCCGGAGGGGAGTGTTAGG+TGG 0.606358 5.3:-19565751 None:intergenic
AAGCTTGCGTCTGTCACCGA+AGG 0.615885 5.3:+19566277 MS.gene054809:CDS
TCCTGAAGGTAATACTGAAG+AGG 0.617871 5.3:+19566210 MS.gene054809:CDS
TGGGGCCTATGAGGTAGGTG+TGG 0.623679 5.3:-19565820 None:intergenic
GCTACGGACGACGGACCAGA+CGG 0.637251 5.3:-19565891 None:intergenic
ACGGACCAGACGGTTTGCGG+CGG 0.644883 5.3:-19565881 None:intergenic
ACCGGTTTCAGTATCATCGG+CGG 0.669189 5.3:+19566076 MS.gene054809:CDS
CTGAAGATTGAGCTTCACAA+CGG 0.671146 5.3:-19566254 None:intergenic
ACCGAAGGAAAGAACCGTGA+CGG 0.674541 5.3:+19566292 MS.gene054809:CDS
AGTCGAATTCGTGTTGACGG+AGG 0.683583 5.3:-19565772 None:intergenic
TTTGTCGAAGCTACGGACGA+CGG 0.690180 5.3:-19565900 None:intergenic
GACGTAAGATTCCAAAACAA+CGG 0.691402 5.3:-19566179 None:intergenic
TTCGGTGACGGTGATAACGG+TGG 0.691549 5.3:+19566145 MS.gene054809:CDS
TGTCATCTCCGGTCTCCCGG+CGG 0.695228 5.3:+19566006 MS.gene054809:CDS
CGTGAACGGAAGTAACGGAA+CGG 0.712612 5.3:-19566120 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGAAGTGTTTGTAGATTTG+CGG - chr5.3:19565926-19565945 None:intergenic 30.0%
! GAAGGTTTTCAAATGAAAGT+CGG + chr5.3:19565965-19565984 MS.gene054809:CDS 30.0%
GACGTAAGATTCCAAAACAA+CGG - chr5.3:19566182-19566201 None:intergenic 35.0%
AAAAGCTGCTCTCTCAAAGA+AGG + chr5.3:19565947-19565966 MS.gene054809:CDS 40.0%
CGGAACGGTAATTCTTCAAA+CGG - chr5.3:19566108-19566127 None:intergenic 40.0%
CTGAAGATTGAGCTTCACAA+CGG - chr5.3:19566257-19566276 None:intergenic 40.0%
TCCTCTTCAGTATTACCTTC+AGG - chr5.3:19566214-19566233 None:intergenic 40.0%
TCCTGAAGGTAATACTGAAG+AGG + chr5.3:19566210-19566229 MS.gene054809:CDS 40.0%
! GTTTTCGTCGGAATCTTGAT+CGG - chr5.3:19565717-19565736 None:intergenic 40.0%
!! GTTACCGGTTTCAGTATCAT+CGG + chr5.3:19566073-19566092 MS.gene054809:CDS 40.0%
!! TGAGTTGATGAAAGGGATGA+GGG - chr5.3:19565798-19565817 None:intergenic 40.0%
!! TGTGGTGTGAGTTGATGAAA+GGG - chr5.3:19565805-19565824 None:intergenic 40.0%
GAAACCGTGAACGGAAGTAA+CGG - chr5.3:19566128-19566147 None:intergenic 45.0%
GTTACTTCCGTTCACGGTTT+CGG + chr5.3:19566127-19566146 MS.gene054809:CDS 45.0%
TACGTCGTTGATGTTCCTGA+AGG + chr5.3:19566196-19566215 MS.gene054809:CDS 45.0%
TTTGCGGTTTGTCGAAGCTA+CGG - chr5.3:19565910-19565929 None:intergenic 45.0%
! TGAGATCTGGACCGTTGTTT+TGG + chr5.3:19566168-19566187 MS.gene054809:CDS 45.0%
!! GTGAGTTGATGAAAGGGATG+AGG - chr5.3:19565799-19565818 None:intergenic 45.0%
!! GTGTGGTGTGAGTTGATGAA+AGG - chr5.3:19565806-19565825 None:intergenic 45.0%
ACCGAAGGAAAGAACCGTGA+CGG + chr5.3:19566292-19566311 MS.gene054809:CDS 50.0%
ACCGCCGATGATACTGAAAC+CGG - chr5.3:19566080-19566099 None:intergenic 50.0%
ACCGTCACGGTTCTTTCCTT+CGG - chr5.3:19566296-19566315 None:intergenic 50.0%
CGTTCCGTTACTTCCGTTCA+CGG + chr5.3:19566121-19566140 MS.gene054809:CDS 50.0%
GGGAGTCGAATTCGTGTTGA+CGG - chr5.3:19565778-19565797 None:intergenic 50.0%
GTCAAGAACGTCGAGTCTCT+CGG - chr5.3:19566041-19566060 None:intergenic 50.0%
TCACACCACACCTACCTCAT+AGG + chr5.3:19565815-19565834 MS.gene054809:CDS 50.0%
TTCGTCGGAATCTTGATCGG+AGG - chr5.3:19565714-19565733 None:intergenic 50.0%
TTTGTCGAAGCTACGGACGA+CGG - chr5.3:19565903-19565922 None:intergenic 50.0%
! ACCGGTTTCAGTATCATCGG+CGG + chr5.3:19566076-19566095 MS.gene054809:CDS 50.0%
! AGTCGAATTCGTGTTGACGG+AGG - chr5.3:19565775-19565794 None:intergenic 50.0%
! CGTGAACGGAAGTAACGGAA+CGG - chr5.3:19566123-19566142 None:intergenic 50.0%
! GGTTTCGGTGACGGTGATAA+CGG + chr5.3:19566142-19566161 MS.gene054809:CDS 50.0%
! TTGTGTGCGGTGGTTTTCGT+CGG - chr5.3:19565729-19565748 None:intergenic 50.0%
!! AGAAGCCTGGAGCATTGGTT+GGG - chr5.3:19565842-19565861 None:intergenic 50.0%
!! GTGATAACGGTGGTGAGATC+TGG + chr5.3:19566155-19566174 MS.gene054809:CDS 50.0%
!! GTGTTAGGTGGTGTTGTGTG+CGG - chr5.3:19565742-19565761 None:intergenic 50.0%
AAGCTTGCGTCTGTCACCGA+AGG + chr5.3:19566277-19566296 MS.gene054809:CDS 55.0%
ACCGTCACCGAAACCGTGAA+CGG - chr5.3:19566137-19566156 None:intergenic 55.0%
CAATGTCATCTCCGGTCTCC+CGG + chr5.3:19566003-19566022 MS.gene054809:CDS 55.0%
CGCGACGTCAATGTCATCTC+CGG + chr5.3:19565995-19566014 MS.gene054809:CDS 55.0%
CTGACTTACCGTCACCGTCA+CGG - chr5.3:19566309-19566328 None:intergenic 55.0%
GACGACGAACGTCGTGTTAC+CGG + chr5.3:19566058-19566077 MS.gene054809:CDS 55.0%
GGAAAGAACCGTGACGGTGA+CGG + chr5.3:19566298-19566317 MS.gene054809:CDS 55.0%
GTCGGAATCTTGATCGGAGG+TGG - chr5.3:19565711-19565730 None:intergenic 55.0%
TCCGTTCACGGTTTCGGTGA+CGG + chr5.3:19566133-19566152 MS.gene054809:CDS 55.0%
TTGATCGGAGGTGGAAGCGA+TGG - chr5.3:19565702-19565721 None:intergenic 55.0%
TTGGTTGGGGCCTATGAGGT+AGG - chr5.3:19565828-19565847 None:intergenic 55.0%
! GAATTCGTGTTGACGGAGGC+CGG - chr5.3:19565771-19565790 None:intergenic 55.0%
!! AGCATTGGTTGGGGCCTATG+AGG - chr5.3:19565832-19565851 None:intergenic 55.0%
!! GAAGCCTGGAGCATTGGTTG+GGG - chr5.3:19565841-19565860 None:intergenic 55.0%
!! GAGAAGCCTGGAGCATTGGT+TGG - chr5.3:19565843-19565862 None:intergenic 55.0%
!! TTAGGTGGTGTTGTGTGCGG+TGG - chr5.3:19565739-19565758 None:intergenic 55.0%
!! TTCGGTGACGGTGATAACGG+TGG + chr5.3:19566145-19566164 MS.gene054809:CDS 55.0%
ACGACGGACCAGACGGTTTG+CGG - chr5.3:19565887-19565906 None:intergenic 60.0%
ATCGGAGGTGGAAGCGATGG+CGG - chr5.3:19565699-19565718 None:intergenic 60.0%
CACCACCTAACACTCCCCTC+CGG + chr5.3:19565749-19565768 MS.gene054809:CDS 60.0%
GAGCGAGAAGCCTGGAGCAT+TGG - chr5.3:19565847-19565866 None:intergenic 60.0%
GATGCGTTGAGCGAGAAGCC+TGG - chr5.3:19565855-19565874 None:intergenic 60.0%
TAGGCCCCAACCAATGCTCC+AGG + chr5.3:19565834-19565853 MS.gene054809:CDS 60.0%
TGGGGCCTATGAGGTAGGTG+TGG - chr5.3:19565823-19565842 None:intergenic 60.0%
ACGGACCAGACGGTTTGCGG+CGG - chr5.3:19565884-19565903 None:intergenic 65.0%
GCTACGGACGACGGACCAGA+CGG - chr5.3:19565894-19565913 None:intergenic 65.0%
TGTCATCTCCGGTCTCCCGG+CGG + chr5.3:19566006-19566025 MS.gene054809:CDS 65.0%
! TCGTGTTGACGGAGGCCGGA+GGG - chr5.3:19565767-19565786 None:intergenic 65.0%
! TTCGTGTTGACGGAGGCCGG+AGG - chr5.3:19565768-19565787 None:intergenic 65.0%
! CGTGTTGACGGAGGCCGGAG+GGG - chr5.3:19565766-19565785 None:intergenic 70.0%
! GGAGGTGGAAGCGATGGCGG+TGG - chr5.3:19565696-19565715 None:intergenic 70.0%
! GGTGGAAGCGATGGCGGTGG+AGG - chr5.3:19565693-19565712 None:intergenic 70.0%
!! CTCTCGGTGCTAGTCGCCGC+CGG - chr5.3:19566025-19566044 None:intergenic 70.0%
!! GGAGGCCGGAGGGGAGTGTT+AGG - chr5.3:19565757-19565776 None:intergenic 70.0%
!! GGCCGGAGGGGAGTGTTAGG+TGG - chr5.3:19565754-19565773 None:intergenic 70.0%
!! TCTCGGTGCTAGTCGCCGCC+GGG - chr5.3:19566024-19566043 None:intergenic 70.0%
ACGCGCCGCCGCAAACCGTC+TGG + chr5.3:19565876-19565895 MS.gene054809:CDS 75.0%
AGACGGTTTGCGGCGGCGCG+TGG - chr5.3:19565877-19565896 None:intergenic 75.0%
GCTAGTCGCCGCCGGGAGAC+CGG - chr5.3:19566017-19566036 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 19565674 19566333 19565674 ID=MS.gene054809
chr5.3 mRNA 19565674 19566333 19565674 ID=MS.gene054809.t1;Parent=MS.gene054809
chr5.3 exon 19565674 19566333 19565674 ID=MS.gene054809.t1.exon1;Parent=MS.gene054809.t1
chr5.3 CDS 19565674 19566333 19565674 ID=cds.MS.gene054809.t1;Parent=MS.gene054809.t1
Gene Sequence

>MS.gene054809

ATGGAGAAAGCAGAGTCCTCCACCGCCATCGCTTCCACCTCCGATCAAGATTCCGACGAAAACCACCGCACACAACACCACCTAACACTCCCCTCCGGCCTCCGTCAACACGAATTCGACTCCCTCATCCCTTTCATCAACTCACACCACACCTACCTCATAGGCCCCAACCAATGCTCCAGGCTTCTCGCTCAACGCATCCACGCGCCGCCGCAAACCGTCTGGTCCGTCGTCCGTAGCTTCGACAAACCGCAAATCTACAAACACTTCATCAAAAGCTGCTCTCTCAAAGAAGGTTTTCAAATGAAAGTCGGTTGCACTCGCGACGTCAATGTCATCTCCGGTCTCCCGGCGGCGACTAGCACCGAGAGACTCGACGTTCTTGACGACGAACGTCGTGTTACCGGTTTCAGTATCATCGGCGGTGAACACCGTTTGAAGAATTACCGTTCCGTTACTTCCGTTCACGGTTTCGGTGACGGTGATAACGGTGGTGAGATCTGGACCGTTGTTTTGGAATCTTACGTCGTTGATGTTCCTGAAGGTAATACTGAAGAGGATACGCGTCTTTTTGCTGATACCGTTGTGAAGCTCAATCTTCAGAAGCTTGCGTCTGTCACCGAAGGAAAGAACCGTGACGGTGACGGTAAGTCAGATTAG

Protein sequence

>MS.gene054809.t1

MEKAESSTAIASTSDQDSDENHRTQHHLTLPSGLRQHEFDSLIPFINSHHTYLIGPNQCSRLLAQRIHAPPQTVWSVVRSFDKPQIYKHFIKSCSLKEGFQMKVGCTRDVNVISGLPAATSTERLDVLDDERRVTGFSIIGGEHRLKNYRSVTSVHGFGDGDNGGEIWTVVLESYVVDVPEGNTEEDTRLFADTVVKLNLQKLASVTEGKNRDGDGKSD